Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018571.01.T01 | XP_003604558.1 | 99.174 | 121 | 1 | 0 | 1 | 121 | 50 | 170 | 1.87E-81 | 246 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018571.01.T01 | Q9M015 | 76.033 | 121 | 29 | 0 | 1 | 121 | 50 | 170 | 2.05E-64 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018571.01.T01 | G7JLS2 | 99.174 | 121 | 1 | 0 | 1 | 121 | 50 | 170 | 8.94e-82 | 246 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049059.01 | MsG0480018571.01 | 0.824067 | 9.868489e-54 | 8.649074e-51 |
| MsG0180004079.01 | MsG0480018571.01 | 0.807739 | 4.361990e-50 | 2.448742e-47 |
| MsG0180004752.01 | MsG0480018571.01 | 0.803104 | 4.086902e-49 | 2.036146e-46 |
| MsG0180004761.01 | MsG0480018571.01 | -0.818220 | 2.195906e-52 | 1.633800e-49 |
| MsG0180005125.01 | MsG0480018571.01 | 0.802423 | 5.650958e-49 | 2.767171e-46 |
| MsG0180006069.01 | MsG0480018571.01 | 0.806571 | 7.711788e-50 | 4.199955e-47 |
| MsG0180006097.01 | MsG0480018571.01 | 0.822822 | 1.928523e-53 | 1.631952e-50 |
| MsG0180006226.01 | MsG0480018571.01 | -0.832101 | 1.145383e-55 | 1.267476e-52 |
| MsG0380014006.01 | MsG0480018571.01 | -0.806912 | 6.531575e-50 | 3.588618e-47 |
| MsG0480018571.01 | MsG0480018998.01 | 0.816522 | 5.296701e-52 | 3.761733e-49 |
| MsG0480018571.01 | MsG0480019038.01 | 0.857355 | 1.726866e-62 | 4.250591e-59 |
| MsG0480018571.01 | MsG0480020052.01 | -0.811994 | 5.298566e-51 | 3.328637e-48 |
| MsG0480018571.01 | MsG0480020372.01 | 0.839198 | 1.829113e-57 | 2.507388e-54 |
| MsG0480018571.01 | MsG0480020444.01 | 0.827283 | 1.703822e-54 | 1.637560e-51 |
| MsG0480018571.01 | MsG0480020445.01 | 0.820314 | 7.322996e-53 | 5.774398e-50 |
| MsG0480018571.01 | MsG0480022274.01 | 0.841426 | 4.786870e-58 | 7.031418e-55 |
| MsG0480018571.01 | MsG0480022479.01 | 0.801906 | 7.217846e-49 | 3.488116e-46 |
| MsG0480018571.01 | MsG0480022480.01 | 0.827848 | 1.246860e-54 | 1.218202e-51 |
| MsG0480018571.01 | MsG0580024035.01 | 0.815853 | 7.473923e-52 | 5.211484e-49 |
| MsG0480018571.01 | MsG0580024476.01 | 0.823226 | 1.552274e-53 | 1.328553e-50 |
| MsG0480018571.01 | MsG0580024570.01 | -0.812394 | 4.335221e-51 | 2.753281e-48 |
| MsG0480018571.01 | MsG0580027626.01 | 0.816319 | 5.880465e-52 | 4.153478e-49 |
| MsG0480018571.01 | MsG0580028605.01 | 0.800060 | 1.720409e-48 | 7.934316e-46 |
| MsG0480018571.01 | MsG0580029583.01 | 0.806660 | 7.382983e-50 | 4.029928e-47 |
| MsG0480018571.01 | MsG0680034061.01 | 0.800015 | 1.757323e-48 | 8.095019e-46 |
| MsG0480018571.01 | MsG0680035741.01 | 0.832673 | 8.266179e-56 | 9.304318e-53 |
| MsG0480018571.01 | MsG0680035777.01 | 0.848806 | 4.847151e-60 | 9.002342e-57 |
| MsG0480018571.01 | MsG0780037599.01 | 0.815142 | 1.075771e-51 | 7.356471e-49 |
| MsG0480018571.01 | MsG0780038118.01 | 0.821787 | 3.352490e-53 | 2.754511e-50 |
| MsG0480018571.01 | MsG0780038444.01 | 0.829733 | 4.362776e-55 | 4.504378e-52 |
| MsG0480018571.01 | MsG0780038495.01 | 0.815026 | 1.141447e-51 | 7.781599e-49 |
| MsG0480018571.01 | MsG0780039452.01 | 0.827009 | 1.981685e-54 | 1.889222e-51 |
| MsG0480018571.01 | MsG0780039463.01 | 0.814437 | 1.541050e-51 | 1.034048e-48 |
| MsG0480018571.01 | MsG0780039841.01 | 0.802938 | 4.423659e-49 | 2.194658e-46 |
| MsG0480018571.01 | MsG0780040861.01 | 0.816225 | 6.169681e-52 | 4.346499e-49 |
| MsG0480018571.01 | MsG0880041883.01 | 0.817980 | 2.489076e-52 | 1.839703e-49 |
| MsG0480018571.01 | MsG0880041985.01 | 0.813754 | 2.180595e-51 | 1.436277e-48 |
| MsG0480018571.01 | MsG0880042811.01 | 0.831539 | 1.576269e-55 | 1.715833e-52 |
| MsG0480018571.01 | MsG0880042843.01 | 0.800165 | 1.638267e-48 | 7.575180e-46 |
| MsG0480018571.01 | MsG0880043262.01 | 0.825515 | 4.495447e-54 | 4.106331e-51 |
| MsG0480018571.01 | MsG0880046088.01 | 0.830661 | 2.590198e-55 | 2.746868e-52 |
| MsG0480018571.01 | MsG0880046669.01 | 0.808617 | 2.835942e-50 | 1.629043e-47 |
| MsG0380015245.01 | MsG0480018571.01 | 0.806625 | 7.511360e-50 | 4.096243e-47 |
| MsG0380015779.01 | MsG0480018571.01 | 0.801929 | 7.140819e-49 | 3.452799e-46 |
| MsG0380015793.01 | MsG0480018571.01 | -0.811282 | 7.567782e-51 | 4.665085e-48 |
| MsG0380017135.01 | MsG0480018571.01 | -0.802740 | 4.860031e-49 | 2.399159e-46 |
| MsG0380017998.01 | MsG0480018571.01 | 0.838762 | 2.372045e-57 | 3.208693e-54 |
| MsG0380018001.01 | MsG0480018571.01 | 0.800296 | 1.540641e-48 | 7.148065e-46 |
| MsG0480018333.01 | MsG0480018571.01 | 0.824798 | 6.640897e-54 | 5.944073e-51 |
| MsG0280007286.01 | MsG0480018571.01 | 0.816367 | 5.735549e-52 | 4.056373e-49 |
| MsG0280007609.01 | MsG0480018571.01 | 0.821460 | 3.991001e-53 | 3.248795e-50 |
| MsG0280008856.01 | MsG0480018571.01 | 0.811543 | 6.642429e-51 | 4.123194e-48 |
| MsG0280009971.01 | MsG0480018571.01 | 0.818434 | 1.964219e-52 | 1.470252e-49 |
| MsG0280010389.01 | MsG0480018571.01 | 0.821733 | 3.450586e-53 | 2.830611e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580026849.01 | MsG0480018571.01 | PPI |
| MsG0580026281.01 | MsG0480018571.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018571.01.T01 | MTR_4g014490 | 99.174 | 121 | 1 | 0 | 1 | 121 | 50 | 170 | 2.27e-85 | 246 |
| MsG0480018571.01.T01 | MTR_7g070000 | 83.471 | 121 | 20 | 0 | 1 | 121 | 50 | 170 | 2.28e-70 | 208 |
| MsG0480018571.01.T01 | MTR_5g043190 | 56.098 | 123 | 51 | 1 | 2 | 121 | 58 | 180 | 8.20e-46 | 146 |
| MsG0480018571.01.T01 | MTR_4g109140 | 48.387 | 124 | 60 | 1 | 2 | 121 | 51 | 174 | 3.58e-37 | 124 |
| MsG0480018571.01.T01 | MTR_7g108775 | 35.354 | 99 | 62 | 2 | 7 | 104 | 33 | 130 | 6.02e-16 | 69.7 |
| MsG0480018571.01.T01 | MTR_7g108775 | 36.842 | 95 | 58 | 2 | 7 | 100 | 33 | 126 | 7.04e-16 | 70.1 |
| MsG0480018571.01.T01 | MTR_1g088250 | 27.368 | 95 | 68 | 1 | 7 | 100 | 26 | 120 | 8.34e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018571.01.T01 | AT3G08890 | 76.860 | 121 | 28 | 0 | 1 | 121 | 50 | 170 | 9.56e-68 | 201 |
| MsG0480018571.01.T01 | AT3G08890 | 76.860 | 121 | 28 | 0 | 1 | 121 | 50 | 170 | 9.56e-68 | 201 |
| MsG0480018571.01.T01 | AT5G37070 | 79.339 | 121 | 25 | 0 | 1 | 121 | 50 | 170 | 1.20e-67 | 201 |
| MsG0480018571.01.T01 | AT5G01610 | 76.033 | 121 | 29 | 0 | 1 | 121 | 50 | 170 | 2.09e-65 | 196 |
| MsG0480018571.01.T01 | AT5G01610 | 76.033 | 121 | 29 | 0 | 1 | 121 | 50 | 170 | 2.09e-65 | 196 |
| MsG0480018571.01.T01 | AT2G03350 | 53.782 | 119 | 52 | 1 | 3 | 118 | 57 | 175 | 1.67e-38 | 128 |
| MsG0480018571.01.T01 | AT1G02816 | 25.217 | 115 | 83 | 2 | 8 | 119 | 35 | 149 | 5.65e-14 | 64.7 |
| MsG0480018571.01.T01 | AT4G02370 | 26.263 | 99 | 72 | 1 | 7 | 104 | 34 | 132 | 4.54e-13 | 62.4 |
| MsG0480018571.01.T01 | AT4G02360 | 29.358 | 109 | 74 | 3 | 2 | 108 | 25 | 132 | 6.45e-12 | 59.3 |
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGACAAAAGTTATTATC+TGG | 0.261455 | 4:+6783163 | MsG0480018571.01.T01:CDS |
| CTTCGATCCCTCGGAGAAAA+TGG | 0.294455 | 4:-6783199 | None:intergenic |
| AGGAAAGCTAGCAGAAATAG+AGG | 0.348692 | 4:+6783137 | MsG0480018571.01.T01:CDS |
| TCCTTTAGCAACTCTGGTAA+TGG | 0.417068 | 4:-6782952 | None:intergenic |
| CGTTTCTTCACCACTGTGAC+TGG | 0.418065 | 4:+6783102 | MsG0480018571.01.T01:CDS |
| GGTCTGCGAAGTAGGCTATA+AGG | 0.432562 | 4:+6783065 | MsG0480018571.01.T01:CDS |
| ATTCCTCAGGTCTGCGAAGT+AGG | 0.457321 | 4:+6783057 | MsG0480018571.01.T01:CDS |
| CACATAGAGCTTCGATCCCT+CGG | 0.465720 | 4:-6783208 | None:intergenic |
| TCACGAGGAAAGATTCCTAT+TGG | 0.473186 | 4:-6782985 | None:intergenic |
| GCTTGAAGTCTTCATTCCTC+AGG | 0.483186 | 4:+6783044 | MsG0480018571.01.T01:CDS |
| AAGGAGTATGATCTGCCAAT+AGG | 0.490338 | 4:+6782970 | MsG0480018571.01.T01:CDS |
| GAAGACAAAAGTTATTATCT+GGG | 0.508796 | 4:+6783164 | MsG0480018571.01.T01:CDS |
| TCATACTCCTTTAGCAACTC+TGG | 0.508816 | 4:-6782958 | None:intergenic |
| GCATATGATGTTACAAGAGA+TGG | 0.523208 | 4:+6783264 | MsG0480018571.01.T01:CDS |
| TCGAAGCTCTATGTGACTGC+TGG | 0.534882 | 4:+6783216 | MsG0480018571.01.T01:CDS |
| GTGACTGGTTATCTAGAGAA+AGG | 0.541935 | 4:+6783117 | MsG0480018571.01.T01:CDS |
| GCATGAAGAAAACAAGGAGT+AGG | 0.544902 | 4:+6783238 | MsG0480018571.01.T01:CDS |
| GCCATTACCAGAGTTGCTAA+AGG | 0.560391 | 4:+6782951 | MsG0480018571.01.T01:CDS |
| CTGCTGGCATGAAGAAAACA+AGG | 0.561931 | 4:+6783232 | MsG0480018571.01.T01:CDS |
| TACGAATTTAACGAAGAGAC+AGG | 0.568869 | 4:+6783018 | MsG0480018571.01.T01:CDS |
| TCGTAGTTTGTTGCATCACG+AGG | 0.584895 | 4:-6783000 | None:intergenic |
| CATGAAGAAAACAAGGAGTA+GGG | 0.600568 | 4:+6783239 | MsG0480018571.01.T01:CDS |
| GGAAAGCTAGCAGAAATAGA+GGG | 0.610143 | 4:+6783138 | MsG0480018571.01.T01:CDS |
| TAGCCTACTTCGCAGACCTG+AGG | 0.660590 | 4:-6783060 | None:intergenic |
| AGTGGTGAAGAAACGCAAGA+CGG | 0.703171 | 4:-6783094 | None:intergenic |
| CTCTAGATAACCAGTCACAG+TGG | 0.705458 | 4:-6783112 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGACAAAAGTTATTATCT+GGG | + | Chr4:6783164-6783183 | MsG0480018571.01.T01:CDS | 25.0% |
| ! | TGAAGACAAAAGTTATTATC+TGG | + | Chr4:6783163-6783182 | MsG0480018571.01.T01:CDS | 25.0% |
| CATGAAGAAAACAAGGAGTA+GGG | + | Chr4:6783239-6783258 | MsG0480018571.01.T01:CDS | 35.0% | |
| GCATATGATGTTACAAGAGA+TGG | + | Chr4:6783264-6783283 | MsG0480018571.01.T01:CDS | 35.0% | |
| TACGAATTTAACGAAGAGAC+AGG | + | Chr4:6783018-6783037 | MsG0480018571.01.T01:CDS | 35.0% | |
| AGGAAAGCTAGCAGAAATAG+AGG | + | Chr4:6783137-6783156 | MsG0480018571.01.T01:CDS | 40.0% | |
| GCATGAAGAAAACAAGGAGT+AGG | + | Chr4:6783238-6783257 | MsG0480018571.01.T01:CDS | 40.0% | |
| GGAAAGCTAGCAGAAATAGA+GGG | + | Chr4:6783138-6783157 | MsG0480018571.01.T01:CDS | 40.0% | |
| TCACGAGGAAAGATTCCTAT+TGG | - | Chr4:6782988-6783007 | None:intergenic | 40.0% | |
| TCATACTCCTTTAGCAACTC+TGG | - | Chr4:6782961-6782980 | None:intergenic | 40.0% | |
| TCCTTTAGCAACTCTGGTAA+TGG | - | Chr4:6782955-6782974 | None:intergenic | 40.0% | |
| ! | AAGGAGTATGATCTGCCAAT+AGG | + | Chr4:6782970-6782989 | MsG0480018571.01.T01:CDS | 40.0% |
| ! | GTGACTGGTTATCTAGAGAA+AGG | + | Chr4:6783117-6783136 | MsG0480018571.01.T01:CDS | 40.0% |
| AGTGGTGAAGAAACGCAAGA+CGG | - | Chr4:6783097-6783116 | None:intergenic | 45.0% | |
| CTCTAGATAACCAGTCACAG+TGG | - | Chr4:6783115-6783134 | None:intergenic | 45.0% | |
| CTGCTGGCATGAAGAAAACA+AGG | + | Chr4:6783232-6783251 | MsG0480018571.01.T01:CDS | 45.0% | |
| GCCATTACCAGAGTTGCTAA+AGG | + | Chr4:6782951-6782970 | MsG0480018571.01.T01:CDS | 45.0% | |
| GCTTGAAGTCTTCATTCCTC+AGG | + | Chr4:6783044-6783063 | MsG0480018571.01.T01:CDS | 45.0% | |
| ! | AGTGACAACCATTTTCTCCG+AGG | + | Chr4:6783191-6783210 | MsG0480018571.01.T01:CDS | 45.0% |
| ! | GTGACAACCATTTTCTCCGA+GGG | + | Chr4:6783192-6783211 | MsG0480018571.01.T01:CDS | 45.0% |
| !! | TCGTAGTTTGTTGCATCACG+AGG | - | Chr4:6783003-6783022 | None:intergenic | 45.0% |
| ATTCCTCAGGTCTGCGAAGT+AGG | + | Chr4:6783057-6783076 | MsG0480018571.01.T01:CDS | 50.0% | |
| CACATAGAGCTTCGATCCCT+CGG | - | Chr4:6783211-6783230 | None:intergenic | 50.0% | |
| CGTTTCTTCACCACTGTGAC+TGG | + | Chr4:6783102-6783121 | MsG0480018571.01.T01:CDS | 50.0% | |
| CTTCGATCCCTCGGAGAAAA+TGG | - | Chr4:6783202-6783221 | None:intergenic | 50.0% | |
| GGTCTGCGAAGTAGGCTATA+AGG | + | Chr4:6783065-6783084 | MsG0480018571.01.T01:CDS | 50.0% | |
| TCGAAGCTCTATGTGACTGC+TGG | + | Chr4:6783216-6783235 | MsG0480018571.01.T01:CDS | 50.0% | |
| TAGCCTACTTCGCAGACCTG+AGG | - | Chr4:6783063-6783082 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 6782943 | 6783308 | 6782943 | ID=MsG0480018571.01;Name=MsG0480018571.01 |
| Chr4 | mRNA | 6782943 | 6783308 | 6782943 | ID=MsG0480018571.01.T01;Parent=MsG0480018571.01;Name=MsG0480018571.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|121 |
| Chr4 | exon | 6782943 | 6783308 | 6782943 | ID=MsG0480018571.01.T01:exon:4924;Parent=MsG0480018571.01.T01 |
| Chr4 | CDS | 6782943 | 6783308 | 6782943 | ID=MsG0480018571.01.T01:cds;Parent=MsG0480018571.01.T01 |
| Gene Sequence |
| Protein sequence |
