Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026620.01.T01 | XP_003614321.2 | 94.382 | 267 | 15 | 0 | 1 | 267 | 167 | 433 | 0 | 514 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026620.01.T01 | A0A396HQQ0 | 94.382 | 267 | 15 | 0 | 1 | 267 | 167 | 433 | 0.0 | 514 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580026620.01 | MsG0580028999.01 | 0.803701 | 3.074177e-49 | 1.555298e-46 |
| MsG0580026620.01 | MsG0880047486.01 | 0.807809 | 4.216543e-50 | 2.371311e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026620.01.T01 | MTR_5g048160 | 94.382 | 267 | 15 | 0 | 1 | 267 | 126 | 392 | 0.0 | 513 |
| MsG0580026620.01.T01 | MTR_0115s0020 | 79.044 | 272 | 46 | 3 | 1 | 266 | 132 | 398 | 2.21e-157 | 444 |
| MsG0580026620.01.T01 | MTR_0064s0160 | 61.710 | 269 | 89 | 6 | 1 | 267 | 167 | 423 | 1.63e-106 | 316 |
| MsG0580026620.01.T01 | MTR_1g061330 | 45.756 | 271 | 111 | 9 | 1 | 266 | 120 | 359 | 1.58e-65 | 209 |
| MsG0580026620.01.T01 | MTR_5g097330 | 27.941 | 272 | 165 | 12 | 2 | 264 | 151 | 400 | 4.17e-18 | 83.6 |
| MsG0580026620.01.T01 | MTR_6g004450 | 28.938 | 273 | 143 | 13 | 14 | 270 | 34 | 271 | 1.01e-16 | 78.2 |
| MsG0580026620.01.T01 | MTR_3g018750 | 26.506 | 249 | 155 | 10 | 30 | 264 | 4 | 238 | 2.63e-14 | 70.9 |
| MsG0580026620.01.T01 | MTR_8g033140 | 25.667 | 300 | 152 | 12 | 2 | 263 | 148 | 414 | 8.06e-14 | 71.2 |
| MsG0580026620.01.T01 | MTR_5g064700 | 24.917 | 301 | 163 | 14 | 2 | 270 | 100 | 369 | 1.51e-13 | 70.1 |
| MsG0580026620.01.T01 | MTR_4g133170 | 27.236 | 246 | 134 | 15 | 2 | 235 | 109 | 321 | 5.16e-13 | 68.6 |
| MsG0580026620.01.T01 | MTR_0129s0070 | 27.660 | 282 | 163 | 14 | 2 | 268 | 140 | 395 | 1.29e-12 | 67.4 |
| MsG0580026620.01.T01 | MTR_7g079370 | 26.556 | 241 | 152 | 10 | 30 | 266 | 133 | 352 | 3.70e-12 | 65.9 |
| MsG0580026620.01.T01 | MTR_5g097680 | 24.490 | 294 | 159 | 15 | 2 | 263 | 161 | 423 | 4.53e-12 | 65.9 |
| MsG0580026620.01.T01 | MTR_5g092850 | 26.104 | 249 | 150 | 10 | 31 | 264 | 170 | 399 | 6.18e-12 | 65.5 |
| MsG0580026620.01.T01 | MTR_1g053510 | 24.662 | 296 | 161 | 11 | 2 | 267 | 143 | 406 | 1.93e-11 | 63.9 |
| MsG0580026620.01.T01 | MTR_4g028510 | 26.613 | 248 | 153 | 11 | 30 | 271 | 180 | 404 | 2.20e-11 | 63.9 |
| MsG0580026620.01.T01 | MTR_4g028660 | 25.979 | 281 | 166 | 12 | 2 | 264 | 140 | 396 | 2.60e-11 | 63.5 |
| MsG0580026620.01.T01 | MTR_4g027450 | 25.979 | 281 | 166 | 12 | 2 | 264 | 140 | 396 | 2.60e-11 | 63.5 |
| MsG0580026620.01.T01 | MTR_8g063440 | 22.628 | 274 | 171 | 10 | 2 | 263 | 135 | 379 | 3.42e-11 | 63.2 |
| MsG0580026620.01.T01 | MTR_8g032980 | 22.549 | 306 | 167 | 11 | 2 | 263 | 138 | 417 | 3.89e-11 | 63.2 |
| MsG0580026620.01.T01 | MTR_2g104280 | 25.685 | 292 | 158 | 14 | 2 | 263 | 139 | 401 | 4.75e-11 | 62.8 |
| MsG0580026620.01.T01 | MTR_5g022850 | 25.806 | 279 | 167 | 11 | 2 | 263 | 94 | 349 | 5.52e-11 | 62.4 |
| MsG0580026620.01.T01 | MTR_5g010560 | 23.954 | 263 | 176 | 7 | 2 | 263 | 196 | 435 | 7.35e-11 | 62.4 |
| MsG0580026620.01.T01 | MTR_2g426090 | 23.077 | 234 | 150 | 9 | 10 | 236 | 143 | 353 | 9.10e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTAAAGATCTTTGGTTAA+AGG | 0.197357 | 5:+40874824 | MsG0580026620.01.T01:CDS |
| CTCACTGATTATGCTAGTTT+TGG | 0.200167 | 5:+40874765 | MsG0580026620.01.T01:CDS |
| GATCTAAAAGTCAAAGATTA+TGG | 0.202937 | 5:+40875404 | MsG0580026620.01.T01:CDS |
| TGTTTCACATTTCCTCTTAT+AGG | 0.224421 | 5:-40875200 | None:intergenic |
| ATTAGAGATTGATAGGCTTA+TGG | 0.263537 | 5:+40875298 | MsG0580026620.01.T01:CDS |
| TGTGAAGACTCACATATTCT+TGG | 0.266244 | 5:-40875116 | None:intergenic |
| TTTGGGTGATGAGAGATTAT+TGG | 0.272724 | 5:+40875231 | MsG0580026620.01.T01:CDS |
| CCTCTTCCCATTGAAATTTG+TGG | 0.275977 | 5:+40874936 | MsG0580026620.01.T01:CDS |
| GAATCTGTCAAATGGGATTC+AGG | 0.285219 | 5:-40874726 | None:intergenic |
| GTTTCACATTTCCTCTTATA+GGG | 0.298777 | 5:-40875199 | None:intergenic |
| TTATTGTGCTTAAAGATCTT+TGG | 0.320771 | 5:+40874817 | MsG0580026620.01.T01:CDS |
| AAGCCTTGTTTCACTTCAAA+TGG | 0.334965 | 5:+40875466 | MsG0580026620.01.T01:CDS |
| TTAGAGATTGATAGGCTTAT+GGG | 0.343549 | 5:+40875299 | MsG0580026620.01.T01:CDS |
| ATTATTGGGATGAAGGTTCT+TGG | 0.345656 | 5:+40875246 | MsG0580026620.01.T01:CDS |
| GATAGGCTTATGGGGTTCAT+TGG | 0.348211 | 5:+40875308 | MsG0580026620.01.T01:CDS |
| ATGTGAAACACTTTGATGTT+TGG | 0.359140 | 5:+40875213 | MsG0580026620.01.T01:CDS |
| TGTGAAACACTTTGATGTTT+GGG | 0.360910 | 5:+40875214 | MsG0580026620.01.T01:CDS |
| GAAACAGATGAAAGACAAAA+AGG | 0.367505 | 5:+40874846 | MsG0580026620.01.T01:CDS |
| TTGGGTGATGAGAGATTATT+GGG | 0.375209 | 5:+40875232 | MsG0580026620.01.T01:CDS |
| CCATTGAATTAGAGATTGAT+AGG | 0.377550 | 5:+40875291 | MsG0580026620.01.T01:CDS |
| TTCATTGTTAACCCTATAAG+AGG | 0.379299 | 5:+40875188 | MsG0580026620.01.T01:CDS |
| TGCCATTTGAATCTGTCAAA+TGG | 0.391432 | 5:-40874734 | None:intergenic |
| ATGAGAGATTATTGGGATGA+AGG | 0.409587 | 5:+40875239 | MsG0580026620.01.T01:CDS |
| ATAGTCTTAATACAAACTCT+TGG | 0.421375 | 5:+40874889 | MsG0580026620.01.T01:CDS |
| CGATGAACCACAAATTTCAA+TGG | 0.425534 | 5:-40874943 | None:intergenic |
| ATAGGCTTATGGGGTTCATT+GGG | 0.433165 | 5:+40875309 | MsG0580026620.01.T01:CDS |
| ATCGTCGTCATCGTCGTCTC+GGG | 0.437997 | 5:+40874962 | MsG0580026620.01.T01:CDS |
| GCCATTTGAATCTGTCAAAT+GGG | 0.439492 | 5:-40874733 | None:intergenic |
| AGCCTTGTTTCACTTCAAAT+GGG | 0.439742 | 5:+40875467 | MsG0580026620.01.T01:CDS |
| CCTATCAATCTCTAATTCAA+TGG | 0.449645 | 5:-40875291 | None:intergenic |
| ATGGTAGAAGATTCTTCAAA+AGG | 0.452659 | 5:-40875161 | None:intergenic |
| TTTGAAGAATCTTCTACCAT+TGG | 0.466919 | 5:+40875164 | MsG0580026620.01.T01:CDS |
| ATCTAAAAGTCAAAGATTAT+GGG | 0.468169 | 5:+40875405 | MsG0580026620.01.T01:CDS |
| CATCGTCGTCATCGTCGTCT+CGG | 0.470421 | 5:+40874961 | MsG0580026620.01.T01:CDS |
| ATTCAGAGCTGTTGTATACA+AGG | 0.489417 | 5:+40875442 | MsG0580026620.01.T01:CDS |
| TCCCATTTGACAGATTCAAA+TGG | 0.517939 | 5:+40874732 | MsG0580026620.01.T01:CDS |
| GATGAACCACAAATTTCAAT+GGG | 0.525210 | 5:-40874942 | None:intergenic |
| CCACAAATTTCAATGGGAAG+AGG | 0.539587 | 5:-40874936 | None:intergenic |
| ATGTAAACAATTGTTGTCAC+TGG | 0.545970 | 5:+40874994 | MsG0580026620.01.T01:CDS |
| AAATTTCAATGGGAAGAGGA+AGG | 0.550406 | 5:-40874932 | None:intergenic |
| AATTTCAATGGGAAGAGGAA+GGG | 0.550665 | 5:-40874931 | None:intergenic |
| CCTTGTTTCACTTCAAATGG+GGG | 0.554854 | 5:+40875469 | MsG0580026620.01.T01:CDS |
| AATGATGATCATGAATCTCA+AGG | 0.574525 | 5:+40875029 | MsG0580026620.01.T01:CDS |
| GAAATGTAATGATGATGAGT+TGG | 0.576947 | 5:+40875349 | MsG0580026620.01.T01:CDS |
| GCCTTGTTTCACTTCAAATG+GGG | 0.582508 | 5:+40875468 | MsG0580026620.01.T01:CDS |
| TAGAGATTGATAGGCTTATG+GGG | 0.588723 | 5:+40875300 | MsG0580026620.01.T01:CDS |
| TAAACAATTGTTGTCACTGG+TGG | 0.606195 | 5:+40874997 | MsG0580026620.01.T01:CDS |
| AGGGTTAACAATGAAACCAA+TGG | 0.625796 | 5:-40875180 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTAAAAGTCAAAGATTAT+GGG | + | Chr5:40875405-40875424 | MsG0580026620.01.T01:CDS | 20.0% |
| ! | ATGAAAGACAAAAAGGATAT+TGG | + | Chr5:40874853-40874872 | MsG0580026620.01.T01:CDS | 25.0% |
| ! | GATCTAAAAGTCAAAGATTA+TGG | + | Chr5:40875404-40875423 | MsG0580026620.01.T01:CDS | 25.0% |
| ! | TTATTGTGCTTAAAGATCTT+TGG | + | Chr5:40874817-40874836 | MsG0580026620.01.T01:CDS | 25.0% |
| !! | ATAGTCTTAATACAAACTCT+TGG | + | Chr5:40874889-40874908 | MsG0580026620.01.T01:CDS | 25.0% |
| !!! | ACTTTGTAGTCATTAGTTTT+TGG | - | Chr5:40874798-40874817 | None:intergenic | 25.0% |
| !!! | CTTTGTAGTCATTAGTTTTT+GGG | - | Chr5:40874797-40874816 | None:intergenic | 25.0% |
| AATGATGATCATGAATCTCA+AGG | + | Chr5:40875029-40875048 | MsG0580026620.01.T01:CDS | 30.0% | |
| ACATGACTCACAAAAAACAA+AGG | + | Chr5:40875382-40875401 | MsG0580026620.01.T01:CDS | 30.0% | |
| ATGGTAGAAGATTCTTCAAA+AGG | - | Chr5:40875164-40875183 | None:intergenic | 30.0% | |
| ATGTAAACAATTGTTGTCAC+TGG | + | Chr5:40874994-40875013 | MsG0580026620.01.T01:CDS | 30.0% | |
| ATGTGAAACACTTTGATGTT+TGG | + | Chr5:40875213-40875232 | MsG0580026620.01.T01:CDS | 30.0% | |
| ATTAGAGATTGATAGGCTTA+TGG | + | Chr5:40875298-40875317 | MsG0580026620.01.T01:CDS | 30.0% | |
| CCATTGAATTAGAGATTGAT+AGG | + | Chr5:40875291-40875310 | MsG0580026620.01.T01:CDS | 30.0% | |
| CCTATCAATCTCTAATTCAA+TGG | - | Chr5:40875294-40875313 | None:intergenic | 30.0% | |
| GAAACAGATGAAAGACAAAA+AGG | + | Chr5:40874846-40874865 | MsG0580026620.01.T01:CDS | 30.0% | |
| GATGAACCACAAATTTCAAT+GGG | - | Chr5:40874945-40874964 | None:intergenic | 30.0% | |
| GCTTAAAGATCTTTGGTTAA+AGG | + | Chr5:40874824-40874843 | MsG0580026620.01.T01:CDS | 30.0% | |
| GTTTCACATTTCCTCTTATA+GGG | - | Chr5:40875202-40875221 | None:intergenic | 30.0% | |
| TGTGAAACACTTTGATGTTT+GGG | + | Chr5:40875214-40875233 | MsG0580026620.01.T01:CDS | 30.0% | |
| TGTTTCACATTTCCTCTTAT+AGG | - | Chr5:40875203-40875222 | None:intergenic | 30.0% | |
| TTAGAGATTGATAGGCTTAT+GGG | + | Chr5:40875299-40875318 | MsG0580026620.01.T01:CDS | 30.0% | |
| TTCATTGTTAACCCTATAAG+AGG | + | Chr5:40875188-40875207 | MsG0580026620.01.T01:CDS | 30.0% | |
| TTTGAAGAATCTTCTACCAT+TGG | + | Chr5:40875164-40875183 | MsG0580026620.01.T01:CDS | 30.0% | |
| ! | GAAATGTAATGATGATGAGT+TGG | + | Chr5:40875349-40875368 | MsG0580026620.01.T01:CDS | 30.0% |
| AAATTTCAATGGGAAGAGGA+AGG | - | Chr5:40874935-40874954 | None:intergenic | 35.0% | |
| AATTTCAATGGGAAGAGGAA+GGG | - | Chr5:40874934-40874953 | None:intergenic | 35.0% | |
| AGGGTTAACAATGAAACCAA+TGG | - | Chr5:40875183-40875202 | None:intergenic | 35.0% | |
| ATGAGAGATTATTGGGATGA+AGG | + | Chr5:40875239-40875258 | MsG0580026620.01.T01:CDS | 35.0% | |
| ATTATTGGGATGAAGGTTCT+TGG | + | Chr5:40875246-40875265 | MsG0580026620.01.T01:CDS | 35.0% | |
| ATTCAGAGCTGTTGTATACA+AGG | + | Chr5:40875442-40875461 | MsG0580026620.01.T01:CDS | 35.0% | |
| CGATGAACCACAAATTTCAA+TGG | - | Chr5:40874946-40874965 | None:intergenic | 35.0% | |
| GCCATTTGAATCTGTCAAAT+GGG | - | Chr5:40874736-40874755 | None:intergenic | 35.0% | |
| TAAACAATTGTTGTCACTGG+TGG | + | Chr5:40874997-40875016 | MsG0580026620.01.T01:CDS | 35.0% | |
| TAGAGATTGATAGGCTTATG+GGG | + | Chr5:40875300-40875319 | MsG0580026620.01.T01:CDS | 35.0% | |
| TCCCATTTGACAGATTCAAA+TGG | + | Chr5:40874732-40874751 | MsG0580026620.01.T01:CDS | 35.0% | |
| TGTGAAGACTCACATATTCT+TGG | - | Chr5:40875119-40875138 | None:intergenic | 35.0% | |
| TTGGGTGATGAGAGATTATT+GGG | + | Chr5:40875232-40875251 | MsG0580026620.01.T01:CDS | 35.0% | |
| TTTGGGTGATGAGAGATTAT+TGG | + | Chr5:40875231-40875250 | MsG0580026620.01.T01:CDS | 35.0% | |
| ! | TGCCATTTGAATCTGTCAAA+TGG | - | Chr5:40874737-40874756 | None:intergenic | 35.0% |
| ! | TTGGGAGTAATCGTTTTCTT+TGG | + | Chr5:40875327-40875346 | MsG0580026620.01.T01:CDS | 35.0% |
| !! | AAGCCTTGTTTCACTTCAAA+TGG | + | Chr5:40875466-40875485 | MsG0580026620.01.T01:CDS | 35.0% |
| !! | AGCCTTGTTTCACTTCAAAT+GGG | + | Chr5:40875467-40875486 | MsG0580026620.01.T01:CDS | 35.0% |
| !! | CTCACTGATTATGCTAGTTT+TGG | + | Chr5:40874765-40874784 | MsG0580026620.01.T01:CDS | 35.0% |
| ATAGGCTTATGGGGTTCATT+GGG | + | Chr5:40875309-40875328 | MsG0580026620.01.T01:CDS | 40.0% | |
| CCACAAATTTCAATGGGAAG+AGG | - | Chr5:40874939-40874958 | None:intergenic | 40.0% | |
| CCTCTTCCCATTGAAATTTG+TGG | + | Chr5:40874936-40874955 | MsG0580026620.01.T01:CDS | 40.0% | |
| GAATCTGTCAAATGGGATTC+AGG | - | Chr5:40874729-40874748 | None:intergenic | 40.0% | |
| ! | CCTTGTTTCACTTCAAATGG+GGG | + | Chr5:40875469-40875488 | MsG0580026620.01.T01:CDS | 40.0% |
| !! | GCCTTGTTTCACTTCAAATG+GGG | + | Chr5:40875468-40875487 | MsG0580026620.01.T01:CDS | 40.0% |
| GATAGGCTTATGGGGTTCAT+TGG | + | Chr5:40875308-40875327 | MsG0580026620.01.T01:CDS | 45.0% | |
| ! | CCCCCATTTGAAGTGAAACA+AGG | - | Chr5:40875472-40875491 | None:intergenic | 45.0% |
| ATCGTCGTCATCGTCGTCTC+GGG | + | Chr5:40874962-40874981 | MsG0580026620.01.T01:CDS | 55.0% | |
| CATCGTCGTCATCGTCGTCT+CGG | + | Chr5:40874961-40874980 | MsG0580026620.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 40874693 | 40875511 | 40874693 | ID=MsG0580026620.01;Name=MsG0580026620.01 |
| Chr5 | mRNA | 40874693 | 40875511 | 40874693 | ID=MsG0580026620.01.T01;Parent=MsG0580026620.01;Name=MsG0580026620.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|272 |
| Chr5 | exon | 40874693 | 40875511 | 40874693 | ID=MsG0580026620.01.T01:exon:6310;Parent=MsG0580026620.01.T01 |
| Chr5 | CDS | 40874693 | 40875511 | 40874693 | ID=MsG0580026620.01.T01:cds;Parent=MsG0580026620.01.T01 |
| Gene Sequence |
| Protein sequence |
