AlfalfaGEDB | MsG0880047486.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047486.01

KEGG: -
GO: -
PFAM: PF05553

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	XP_003610146.1	96.212	132	5	0	1	132	1	132	1.53E-87	261

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	G7JD05	96.212	132	5	0	1	132	1	132	7.31e-88	261

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0880047486.01	0.861232	1.185510e-63	3.330933e-60
MsG0080048347.01	MsG0880047486.01	0.828474	8.812590e-55	8.768575e-52
MsG0080048348.01	MsG0880047486.01	0.835017	2.142794e-56	2.587314e-53
MsG0080048349.01	MsG0880047486.01	0.821955	3.066228e-53	2.531186e-50
MsG0080048350.01	MsG0880047486.01	0.823704	1.200270e-53	1.041457e-50
MsG0080048352.01	MsG0880047486.01	0.839465	1.559059e-57	2.154842e-54
MsG0180000019.01	MsG0880047486.01	0.841709	4.030629e-58	5.972168e-55
MsG0180000337.01	MsG0880047486.01	0.852644	4.031404e-61	8.479816e-58
MsG0180000930.01	MsG0880047486.01	0.841113	5.785041e-58	8.413838e-55
MsG0180001050.01	MsG0880047486.01	0.826202	3.086652e-54	2.875444e-51
MsG0180001053.01	MsG0880047486.01	0.839999	1.131994e-57	1.590852e-54
MsG0180002439.01	MsG0880047486.01	-0.804906	1.725332e-49	9.004822e-47
MsG0880044570.01	MsG0880047486.01	0.825712	4.036268e-54	3.707881e-51
MsG0880044669.01	MsG0880047486.01	0.818158	2.267991e-52	1.684590e-49
MsG0880045272.01	MsG0880047486.01	0.840841	6.820030e-58	9.836917e-55
MsG0880045275.01	MsG0880047486.01	0.816691	4.853725e-52	3.462972e-49
MsG0880045277.01	MsG0880047486.01	0.828532	8.531786e-55	8.503635e-52
MsG0880045441.01	MsG0880047486.01	0.816464	5.454968e-52	3.867911e-49
MsG0880045677.01	MsG0880047486.01	0.821106	4.817169e-53	3.883184e-50
MsG0880046176.01	MsG0880047486.01	0.821296	4.355053e-53	3.529002e-50
MsG0880046429.01	MsG0880047486.01	0.801228	9.941339e-49	4.722544e-46
MsG0880046863.01	MsG0880047486.01	0.818289	2.118744e-52	1.579389e-49
MsG0880046996.01	MsG0880047486.01	0.866281	3.196706e-65	1.071528e-61
MsG0880047081.01	MsG0880047486.01	0.841718	4.009623e-58	5.942521e-55
MsG0880047256.01	MsG0880047486.01	0.806459	8.142845e-50	4.422209e-47
MsG0880047486.01	MsG0880047491.01	0.915391	6.223160e-85	1.622005e-80
MsG0180003240.01	MsG0880047486.01	0.818146	2.282177e-52	1.694570e-49
MsG0180003248.01	MsG0880047486.01	0.806576	7.694006e-50	4.190724e-47
MsG0180003687.01	MsG0880047486.01	0.804394	2.206443e-49	1.136326e-46
MsG0180003830.01	MsG0880047486.01	0.814941	1.192284e-51	8.109102e-49
MsG0180004110.01	MsG0880047486.01	0.807696	4.456764e-50	2.498938e-47
MsG0180004203.01	MsG0880047486.01	0.824064	9.880479e-54	8.659001e-51
MsG0180004562.01	MsG0880047486.01	0.887615	1.221573e-72	9.148844e-69
MsG0180004752.01	MsG0880047486.01	0.831433	1.674396e-55	1.816647e-52
MsG0180004941.01	MsG0880047486.01	0.804020	2.639849e-49	1.346589e-46
MsG0180004942.01	MsG0880047486.01	0.821349	4.232496e-53	3.434899e-50
MsG0180005328.01	MsG0880047486.01	0.812935	3.300776e-51	2.126249e-48
MsG0180005356.01	MsG0880047486.01	0.848827	4.780719e-60	8.885201e-57
MsG0180005357.01	MsG0880047486.01	0.859955	2.890936e-63	7.772557e-60
MsG0180005358.01	MsG0880047486.01	0.836158	1.102159e-56	1.377398e-53
MsG0780040480.01	MsG0880047486.01	0.823295	1.495839e-53	1.282764e-50
MsG0780041079.01	MsG0880047486.01	0.801903	7.226697e-49	3.492170e-46
MsG0780041198.01	MsG0880047486.01	0.812208	4.759609e-51	3.007624e-48
MsG0780041299.01	MsG0880047486.01	0.825066	5.740982e-54	5.177741e-51
MsG0780041316.01	MsG0880047486.01	0.844025	9.760089e-59	1.555527e-55
MsG0780041556.01	MsG0880047486.01	0.861051	1.345709e-63	3.756753e-60
MsG0780041574.01	MsG0880047486.01	0.830852	2.325378e-55	2.479992e-52
MsG0880042810.01	MsG0880047486.01	0.830267	3.233747e-55	3.390249e-52
MsG0880043109.01	MsG0880047486.01	0.806850	6.733497e-50	3.693708e-47
MsG0880043185.01	MsG0880047486.01	0.819289	1.255953e-52	9.624333e-50
MsG0880043685.01	MsG0880047486.01	0.823850	1.109047e-53	9.661781e-51
MsG0880043715.01	MsG0880047486.01	0.825703	4.056538e-54	3.725566e-51
MsG0880043744.01	MsG0880047486.01	0.809811	1.573208e-50	9.328787e-48
MsG0880043824.01	MsG0880047486.01	0.805521	1.282464e-49	6.799133e-47
MsG0880043828.01	MsG0880047486.01	0.837070	6.455667e-57	8.291436e-54
MsG0280011041.01	MsG0880047486.01	0.804143	2.488424e-49	1.273359e-46
MsG0280011042.01	MsG0880047486.01	0.819455	1.150853e-52	8.860767e-50
MsG0280011053.01	MsG0880047486.01	0.811585	6.504062e-51	4.041611e-48
MsG0280011165.01	MsG0880047486.01	0.854737	1.008367e-61	2.274386e-58
MsG0280011193.01	MsG0880047486.01	0.836776	7.673451e-57	9.769323e-54
MsG0280011203.01	MsG0880047486.01	0.825936	3.571759e-54	3.302326e-51
MsG0380011613.01	MsG0880047486.01	0.865113	7.471144e-65	2.402744e-61
MsG0380013272.01	MsG0880047486.01	0.831299	1.806134e-55	1.951797e-52
MsG0380013278.01	MsG0880047486.01	0.827995	1.149683e-54	1.128111e-51
MsG0380013300.01	MsG0880047486.01	0.845820	3.199736e-59	5.399095e-56
MsG0680030961.01	MsG0880047486.01	0.830811	2.380045e-55	2.535275e-52
MsG0680031429.01	MsG0880047486.01	0.802549	5.321601e-49	2.614145e-46
MsG0680035496.01	MsG0880047486.01	0.803321	3.685714e-49	1.846544e-46
MsG0480018500.01	MsG0880047486.01	0.802480	5.499980e-49	2.697006e-46
MsG0480018807.01	MsG0880047486.01	0.810652	1.036330e-50	6.282656e-48
MsG0480019105.01	MsG0880047486.01	0.803203	3.898502e-49	1.947289e-46
MsG0480019295.01	MsG0880047486.01	0.831703	1.436679e-55	1.571493e-52
MsG0480019726.01	MsG0880047486.01	0.845703	3.441889e-59	5.785965e-56
MsG0480020497.01	MsG0880047486.01	0.802498	5.453751e-49	2.675512e-46
MsG0480021131.01	MsG0880047486.01	0.818496	1.901593e-52	1.425713e-49
MsG0480021135.01	MsG0880047486.01	0.823462	1.367560e-53	1.178260e-50
MsG0480022125.01	MsG0880047486.01	0.861326	1.110247e-63	3.129892e-60
MsG0480022400.01	MsG0880047486.01	0.802326	5.915621e-49	2.889692e-46
MsG0480022736.01	MsG0880047486.01	0.812532	4.042707e-51	2.576941e-48
MsG0480022797.01	MsG0880047486.01	0.805881	1.077582e-49	5.766338e-47
MsG0480023107.01	MsG0880047486.01	0.845439	4.059326e-59	6.764856e-56
MsG0580024154.01	MsG0880047486.01	0.810342	1.209158e-50	7.269964e-48
MsG0580024408.01	MsG0880047486.01	0.814807	1.276431e-51	8.650537e-49
MsG0580024871.01	MsG0880047486.01	0.815843	7.510907e-52	5.235877e-49
MsG0580025876.01	MsG0880047486.01	0.823695	1.206042e-53	1.046204e-50
MsG0380015010.01	MsG0880047486.01	0.831911	1.276515e-55	1.404932e-52
MsG0380015572.01	MsG0880047486.01	0.819039	1.431813e-52	1.089770e-49
MsG0380016270.01	MsG0880047486.01	0.807630	4.602738e-50	2.576448e-47
MsG0380016494.01	MsG0880047486.01	0.813968	1.956388e-51	1.296098e-48
MsG0380016839.01	MsG0880047486.01	0.849645	2.830777e-60	5.402346e-57
MsG0380017550.01	MsG0880047486.01	0.813638	2.313158e-51	1.518787e-48
MsG0380017690.01	MsG0880047486.01	0.831836	1.331738e-55	1.462479e-52
MsG0380017766.01	MsG0880047486.01	0.802420	5.658762e-49	2.770800e-46
MsG0380018051.01	MsG0880047486.01	0.800213	1.601759e-48	7.415700e-46
MsG0580026620.01	MsG0880047486.01	0.807809	4.216543e-50	2.371311e-47
MsG0580027646.01	MsG0880047486.01	0.817830	2.689713e-52	1.980033e-49
MsG0580027733.01	MsG0880047486.01	0.813021	3.159988e-51	2.040353e-48
MsG0580028758.01	MsG0880047486.01	0.805796	1.122929e-49	5.995908e-47
MsG0580028999.01	MsG0880047486.01	0.865622	5.168145e-65	1.692697e-61
MsG0580029555.01	MsG0880047486.01	0.821540	3.825044e-53	3.120733e-50
MsG0580029583.01	MsG0880047486.01	0.855237	7.219742e-62	1.655538e-58
MsG0580029930.01	MsG0880047486.01	0.835125	2.013033e-56	2.438422e-53
MsG0680030315.01	MsG0880047486.01	0.803908	2.784329e-49	1.416284e-46
MsG0680030545.01	MsG0880047486.01	0.837649	4.589478e-57	6.001118e-54
MsG0280006883.01	MsG0880047486.01	0.853389	2.468885e-61	5.325736e-58
MsG0280007759.01	MsG0880047486.01	0.860709	1.709196e-63	4.716123e-60
MsG0280007760.01	MsG0880047486.01	0.841347	5.022674e-58	7.358694e-55
MsG0280008405.01	MsG0880047486.01	0.842090	3.196519e-58	4.794042e-55
MsG0280009394.01	MsG0880047486.01	0.878840	2.027509e-69	1.074440e-65
MsG0680035841.01	MsG0880047486.01	0.827300	1.688558e-54	1.623686e-51
MsG0780036661.01	MsG0880047486.01	0.805932	1.051341e-49	5.633284e-47
MsG0780037182.01	MsG0880047486.01	0.808844	2.536141e-50	1.465561e-47
MsG0780038123.01	MsG0880047486.01	0.836058	1.168901e-56	1.456331e-53
MsG0780038722.01	MsG0880047486.01	0.801587	8.392261e-49	4.022776e-46
MsG0780038922.01	MsG0880047486.01	0.802247	6.141878e-49	2.994065e-46
MsG0780038924.01	MsG0880047486.01	0.834699	2.577394e-56	3.082122e-53
MsG0780039190.01	MsG0880047486.01	0.811290	7.537874e-51	4.647609e-48
MsG0780039334.01	MsG0880047486.01	0.836093	1.145364e-56	1.428555e-53
MsG0780039877.01	MsG0880047486.01	0.824509	7.768231e-54	6.894889e-51
MsG0780039968.01	MsG0880047486.01	0.830821	2.366397e-55	2.521504e-52
MsG0780039978.01	MsG0880047486.01	0.846718	1.821387e-59	3.163768e-56
MsG0780040144.01	MsG0880047486.01	0.833850	4.208908e-56	4.907675e-53
MsG0780040181.01	MsG0880047486.01	0.802664	5.038965e-49	2.482515e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	MTR_4g128470	96.212	132	5	0	1	132	1	132	1.85e-91	261

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	AT5G57510	47.200	125	60	5	6	127	3	124	3.96e-25	93.2

Find 19 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAGGGAACAAAAGAGTTC+TGG	0.299960	8:-86999243	MsG0880047486.01.T01:CDS
CATTGCAGCTACGTTATTGC+AGG	0.354632	8:-86999260	MsG0880047486.01.T01:CDS
CTCCATCTCCTTGTCTTCAA+AGG	0.416512	8:-86999380	MsG0880047486.01.T01:CDS
GTTCCATGTGTGGTTGAGTA+TGG	0.434971	8:+86999515	None:intergenic
CCTACGAACGGCGGTGCAAA+AGG	0.470375	8:-86999559	MsG0880047486.01.T01:CDS
CTTATTGTCCTTTGAAGACA+AGG	0.489660	8:+86999372	None:intergenic
CTAAGTTTCAACGATCGTCC+TGG	0.511331	8:-86999450	MsG0880047486.01.T01:CDS
GTCCTTTGAAGACAAGGAGA+TGG	0.521798	8:+86999378	None:intergenic
TTCAGATGAAACTGATTCAG+AGG	0.544944	8:-86999412	MsG0880047486.01.T01:CDS
CCACCATACTCAACCACACA+TGG	0.550819	8:-86999518	MsG0880047486.01.T01:CDS
TTTGCACCGCCGTTCGTAGG+CGG	0.568746	8:+86999562	None:intergenic
TCAGATGAAACTGATTCAGA+GGG	0.570568	8:-86999411	MsG0880047486.01.T01:CDS
GAAGAACACATCATCAATCC+AGG	0.580359	8:+86999432	None:intergenic
CCATGTGTGGTTGAGTATGG+TGG	0.585997	8:+86999518	None:intergenic
GGATGACATTGATAAGAGAT+CGG	0.600101	8:-86999334	MsG0880047486.01.T01:CDS
TCAATGTCATCCTCATCTGA+AGG	0.601031	8:+86999345	None:intergenic
ATTGCAGCTACGTTATTGCA+GGG	0.621027	8:-86999259	MsG0880047486.01.T01:CDS
TCTACTAAGCCGCCTACGAA+CGG	0.672450	8:-86999571	MsG0880047486.01.T01:CDS
ACTAAGCCGCCTACGAACGG+CGG	0.714763	8:-86999568	MsG0880047486.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATAAGTTTTCCTTCAGATG+AGG	-	Chr8:86999475-86999494	MsG0880047486.01.T01:CDS	30.0%
	GGATGACATTGATAAGAGAT+CGG	-	Chr8:86999496-86999515	MsG0880047486.01.T01:CDS	35.0%
!	CTTATTGTCCTTTGAAGACA+AGG	+	Chr8:86999461-86999480	None:intergenic	35.0%
!	TCAGATGAAACTGATTCAGA+GGG	-	Chr8:86999419-86999438	MsG0880047486.01.T01:CDS	35.0%
!	TTCAGATGAAACTGATTCAG+AGG	-	Chr8:86999418-86999437	MsG0880047486.01.T01:CDS	35.0%
	ATTGCAGCTACGTTATTGCA+GGG	-	Chr8:86999571-86999590	MsG0880047486.01.T01:CDS	40.0%
	GAAGAACACATCATCAATCC+AGG	+	Chr8:86999401-86999420	None:intergenic	40.0%
	TCAATGTCATCCTCATCTGA+AGG	+	Chr8:86999488-86999507	None:intergenic	40.0%
	AACGCTGCAAAAATGCTTCG+CGG	-	Chr8:86999335-86999354	MsG0880047486.01.T01:CDS	45.0%
	CATTGCAGCTACGTTATTGC+AGG	-	Chr8:86999570-86999589	MsG0880047486.01.T01:CDS	45.0%
	CTAAGTTTCAACGATCGTCC+TGG	-	Chr8:86999380-86999399	MsG0880047486.01.T01:CDS	45.0%
	CTCCATCTCCTTGTCTTCAA+AGG	-	Chr8:86999450-86999469	MsG0880047486.01.T01:CDS	45.0%
	GTTCCATGTGTGGTTGAGTA+TGG	+	Chr8:86999318-86999337	None:intergenic	45.0%
	TGCAGGGAACAAAAGAGTTC+TGG	-	Chr8:86999587-86999606	MsG0880047486.01.T01:CDS	45.0%
!	GTCCTTTGAAGACAAGGAGA+TGG	+	Chr8:86999455-86999474	None:intergenic	45.0%
!	TTTTTGCAGCGTTCCATGTG+TGG	+	Chr8:86999328-86999347	None:intergenic	45.0%
	CCACCATACTCAACCACACA+TGG	-	Chr8:86999312-86999331	MsG0880047486.01.T01:CDS	50.0%
	CCATGTGTGGTTGAGTATGG+TGG	+	Chr8:86999315-86999334	None:intergenic	50.0%
	TCTACTAAGCCGCCTACGAA+CGG	-	Chr8:86999259-86999278	MsG0880047486.01.T01:CDS	50.0%
	ACTAAGCCGCCTACGAACGG+CGG	-	Chr8:86999262-86999281	MsG0880047486.01.T01:CDS	60.0%
	CCTACGAACGGCGGTGCAAA+AGG	-	Chr8:86999271-86999290	MsG0880047486.01.T01:CDS	60.0%
	TTTGCACCGCCGTTCGTAGG+CGG	+	Chr8:86999271-86999290	None:intergenic	60.0%
!	CCTTTTGCACCGCCGTTCGT+AGG	+	Chr8:86999274-86999293	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	86999227	86999625	86999227	ID=MsG0880047486.01;Name=MsG0880047486.01
Chr8	mRNA	86999227	86999625	86999227	ID=MsG0880047486.01.T01;Parent=MsG0880047486.01;Name=MsG0880047486.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|132
Chr8	exon	86999227	86999625	86999227	ID=MsG0880047486.01.T01:exon:18560;Parent=MsG0880047486.01.T01
Chr8	CDS	86999227	86999625	86999227	ID=MsG0880047486.01.T01:cds;Parent=MsG0880047486.01.T01

Gene Sequence

>MsG0880047486.01.T01

ATGAGCGCACACAATCAAAAGCAGAACAAGTCTCTACTAAGCCGCCTACGAACGGCGGTGCAAAAGGCGAAGCTTCTTATAAGCTCCACCATACTCAACCACACATGGAACGCTGCAAAAATGCTTCGCGGTGCTTCTTTGAGCAATCGACAACTAAGTTTCAACGATCGTCCTGGATTGATGATGTGTTCTTCAGATGAAACTGATTCAGAGGGTTCTGTTTCTCCATCTCCTTGTCTTCAAAGGACAATAAGTTTTCCTTCAGATGAGGATGACATTGATAAGAGATCGGAGATTTTCATAGCTAATTTCAGACGACAACTTTTGTTAGAGAGACAAATCTCATTGCAGCTACGTTATTGCAGGGAACAAAAGAGTTCTGGATTGATATCTCCTTGA

Protein sequence

>MsG0880047486.01.T01

MSAHNQKQNKSLLSRLRTAVQKAKLLISSTILNHTWNAAKMLRGASLSNRQLSFNDRPGLMMCSSDETDSEGSVSPSPCLQRTISFPSDEDDIDKRSEIFIANFRRQLLLERQISLQLRYCREQKSSGLISP*