AlfalfaGEDB | MsG0880047486.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047486.01

KEGG: -
GO: -
PFAM: PF05553

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	XP_003610146.1	96.212	132	5	0	1	132	1	132	1.53E-87	261

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	G7JD05	96.212	132	5	0	1	132	1	132	7.31e-88	261

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0880047486.01	0.861232	1.185510e-63	3.330933e-60
MsG0080048347.01	MsG0880047486.01	0.828474	8.812590e-55	8.768575e-52
MsG0080048348.01	MsG0880047486.01	0.835017	2.142794e-56	2.587314e-53
MsG0080048349.01	MsG0880047486.01	0.821955	3.066228e-53	2.531186e-50
MsG0080048350.01	MsG0880047486.01	0.823704	1.200270e-53	1.041457e-50
MsG0080048352.01	MsG0880047486.01	0.839465	1.559059e-57	2.154842e-54
MsG0180000019.01	MsG0880047486.01	0.841709	4.030629e-58	5.972168e-55
MsG0180000337.01	MsG0880047486.01	0.852644	4.031404e-61	8.479816e-58
MsG0180000930.01	MsG0880047486.01	0.841113	5.785041e-58	8.413838e-55
MsG0180001050.01	MsG0880047486.01	0.826202	3.086652e-54	2.875444e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	MTR_4g128470	96.212	132	5	0	1	132	1	132	1.85e-91	261

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047486.01.T01	AT5G57510	47.200	125	60	5	6	127	3	124	3.96e-25	93.2

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Find 19 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTAAGCCGCCTACGAACGG+CGG	0.714763	8:-86999568	MsG0880047486.01.T01:CDS
ATTGCAGCTACGTTATTGCA+GGG	0.621027	8:-86999259	MsG0880047486.01.T01:CDS
CATTGCAGCTACGTTATTGC+AGG	0.354632	8:-86999260	MsG0880047486.01.T01:CDS
CCACCATACTCAACCACACA+TGG	0.550819	8:-86999518	MsG0880047486.01.T01:CDS
CCATGTGTGGTTGAGTATGG+TGG	0.585997	8:+86999518	None:intergenic
CCTACGAACGGCGGTGCAAA+AGG	0.470375	8:-86999559	MsG0880047486.01.T01:CDS
CTAAGTTTCAACGATCGTCC+TGG	0.511331	8:-86999450	MsG0880047486.01.T01:CDS
CTCCATCTCCTTGTCTTCAA+AGG	0.416512	8:-86999380	MsG0880047486.01.T01:CDS
CTTATTGTCCTTTGAAGACA+AGG	0.489660	8:+86999372	None:intergenic
GAAGAACACATCATCAATCC+AGG	0.580359	8:+86999432	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGATGACATTGATAAGAGAT+CGG	-	Chr8:86999496-86999515	MsG0880047486.01.T01:CDS	35.0%
ATTGCAGCTACGTTATTGCA+GGG	-	Chr8:86999571-86999590	MsG0880047486.01.T01:CDS	40.0%
GAAGAACACATCATCAATCC+AGG	+	Chr8:86999401-86999420	None:intergenic	40.0%
TCAATGTCATCCTCATCTGA+AGG	+	Chr8:86999488-86999507	None:intergenic	40.0%
AACGCTGCAAAAATGCTTCG+CGG	-	Chr8:86999335-86999354	MsG0880047486.01.T01:CDS	45.0%
CATTGCAGCTACGTTATTGC+AGG	-	Chr8:86999570-86999589	MsG0880047486.01.T01:CDS	45.0%
CTAAGTTTCAACGATCGTCC+TGG	-	Chr8:86999380-86999399	MsG0880047486.01.T01:CDS	45.0%
CTCCATCTCCTTGTCTTCAA+AGG	-	Chr8:86999450-86999469	MsG0880047486.01.T01:CDS	45.0%
GTTCCATGTGTGGTTGAGTA+TGG	+	Chr8:86999318-86999337	None:intergenic	45.0%
TGCAGGGAACAAAAGAGTTC+TGG	-	Chr8:86999587-86999606	MsG0880047486.01.T01:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	86999227	86999625	86999227	ID=MsG0880047486.01;Name=MsG0880047486.01
Chr8	mRNA	86999227	86999625	86999227	ID=MsG0880047486.01.T01;Parent=MsG0880047486.01;Name=MsG0880047486.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|132
Chr8	exon	86999227	86999625	86999227	ID=MsG0880047486.01.T01:exon:18560;Parent=MsG0880047486.01.T01
Chr8	CDS	86999227	86999625	86999227	ID=MsG0880047486.01.T01:cds;Parent=MsG0880047486.01.T01

Gene Sequence

>MsG0880047486.01.T01

ATGAGCGCACACAATCAAAAGCAGAACAAGTCTCTACTAAGCCGCCTACGAACGGCGGTGCAAAAGGCGAAGCTTCTTATAAGCTCCACCATACTCAACCACACATGGAACGCTGCAAAAATGCTTCGCGGTGCTTCTTTGAGCAATCGACAACTAAGTTTCAACGATCGTCCTGGATTGATGATGTGTTCTTCAGATGAAACTGATTCAGAGGGTTCTGTTTCTCCATCTCCTTGTCTTCAAAGGACAATAAGTTTTCCTTCAGATGAGGATGACATTGATAAGAGATCGGAGATTTTCATAGCTAATTTCAGACGACAACTTTTGTTAGAGAGACAAATCTCATTGCAGCTACGTTATTGCAGGGAACAAAAGAGTTCTGGATTGATATCTCCTTGA

Protein sequence

>MsG0880047486.01.T01

MSAHNQKQNKSLLSRLRTAVQKAKLLISSTILNHTWNAAKMLRGASLSNRQLSFNDRPGLMMCSSDETDSEGSVSPSPCLQRTISFPSDEDDIDKRSEIFIANFRRQLLLERQISLQLRYCREQKSSGLISP*