Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026867.01.T01 | XP_039686533.1 | 96.522 | 115 | 4 | 0 | 1 | 115 | 15 | 129 | 2.78E-69 | 228 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026867.01.T01 | A0A396IPL5 | 98.261 | 115 | 2 | 0 | 1 | 115 | 15 | 129 | 3.72e-69 | 231 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014947.01 | MsG0580026867.01 | 0.801558 | 8.509313e-49 | 4.075984e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026867.01.T01 | MTR_8g054490 | 93.407 | 91 | 6 | 0 | 1 | 91 | 15 | 105 | 9.68e-47 | 150 |
| MsG0580026867.01.T01 | MTR_7g034745 | 93.750 | 80 | 5 | 0 | 1 | 80 | 15 | 94 | 1.40e-41 | 132 |
| MsG0580026867.01.T01 | MTR_4g102460 | 69.027 | 113 | 35 | 0 | 1 | 113 | 14 | 126 | 6.64e-41 | 142 |
| MsG0580026867.01.T01 | MTR_7g105270 | 68.750 | 112 | 35 | 0 | 4 | 115 | 1 | 112 | 4.31e-36 | 128 |
| MsG0580026867.01.T01 | MTR_1g105095 | 62.136 | 103 | 39 | 0 | 13 | 115 | 76 | 178 | 5.24e-36 | 121 |
| MsG0580026867.01.T01 | MTR_7g056297 | 65.455 | 110 | 38 | 0 | 4 | 113 | 1 | 110 | 1.38e-35 | 126 |
| MsG0580026867.01.T01 | MTR_0045s0120 | 66.292 | 89 | 30 | 0 | 4 | 92 | 1 | 89 | 2.07e-24 | 89.7 |
| MsG0580026867.01.T01 | MTR_7g060240 | 59.091 | 88 | 35 | 1 | 1 | 88 | 16 | 102 | 8.36e-20 | 77.8 |
| MsG0580026867.01.T01 | MTR_1361s0010 | 74.286 | 35 | 9 | 0 | 79 | 113 | 1 | 35 | 3.35e-11 | 58.5 |
| MsG0580026867.01.T01 | MTR_2g076860 | 33.333 | 81 | 54 | 0 | 32 | 112 | 76 | 156 | 4.69e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAAACATGCTTCAATT+TGG | 0.257143 | 5:+46795948 | MsG0580026867.01.T01:CDS |
| TCTCTGAATGATTGTCTTTG+TGG | 0.337173 | 5:-46795782 | None:intergenic |
| CAAAGACCCGTTCAACAGTT+TGG | 0.339906 | 5:-46795874 | None:intergenic |
| TGTAGCTTTCCTTCAAAATC+AGG | 0.348866 | 5:-46795827 | None:intergenic |
| AAATGCACAACAAGCACAAT+TGG | 0.413256 | 5:+46795691 | MsG0580026867.01.T01:CDS |
| TTACAGTTTCTTGTAACTGT+TGG | 0.421464 | 5:-46795670 | None:intergenic |
| AGCCAATCAATGAACTCATC+TGG | 0.442778 | 5:-46795851 | None:intergenic |
| TTGGCTCCAAACTGTTGAAC+GGG | 0.451354 | 5:+46795868 | MsG0580026867.01.T01:CDS |
| ATCCAGATGAGTTCATTGAT+TGG | 0.473409 | 5:+46795849 | MsG0580026867.01.T01:CDS |
| AGAAACATGCTTCAATTTGG+TGG | 0.496507 | 5:+46795951 | MsG0580026867.01.T01:CDS |
| GAAACATGCTTCAATTTGGT+GGG | 0.515877 | 5:+46795952 | MsG0580026867.01.T01:CDS |
| ATTGGCTCCAAACTGTTGAA+CGG | 0.582920 | 5:+46795867 | MsG0580026867.01.T01:CDS |
| ATTCAGAGACAACGACATCA+AGG | 0.588760 | 5:+46795796 | MsG0580026867.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAGAAACATGCTTCAATT+TGG | + | Chr5:46795948-46795967 | MsG0580026867.01.T01:CDS | 30.0% | |
| TTACAGTTTCTTGTAACTGT+TGG | - | Chr5:46795673-46795692 | None:intergenic | 30.0% | |
| ! | GTAGATATTCCTGATTTTGA+AGG | + | Chr5:46795818-46795837 | MsG0580026867.01.T01:CDS | 30.0% |
| AAATGCACAACAAGCACAAT+TGG | + | Chr5:46795691-46795710 | MsG0580026867.01.T01:CDS | 35.0% | |
| AGAAACATGCTTCAATTTGG+TGG | + | Chr5:46795951-46795970 | MsG0580026867.01.T01:CDS | 35.0% | |
| ATCCAGATGAGTTCATTGAT+TGG | + | Chr5:46795849-46795868 | MsG0580026867.01.T01:CDS | 35.0% | |
| GAAACATGCTTCAATTTGGT+GGG | + | Chr5:46795952-46795971 | MsG0580026867.01.T01:CDS | 35.0% | |
| TCTCTGAATGATTGTCTTTG+TGG | - | Chr5:46795785-46795804 | None:intergenic | 35.0% | |
| TGTAGCTTTCCTTCAAAATC+AGG | - | Chr5:46795830-46795849 | None:intergenic | 35.0% | |
| AGCCAATCAATGAACTCATC+TGG | - | Chr5:46795854-46795873 | None:intergenic | 40.0% | |
| ATTCAGAGACAACGACATCA+AGG | + | Chr5:46795796-46795815 | MsG0580026867.01.T01:CDS | 40.0% | |
| ! | ATTGGCTCCAAACTGTTGAA+CGG | + | Chr5:46795867-46795886 | MsG0580026867.01.T01:CDS | 40.0% |
| CAAAGACCCGTTCAACAGTT+TGG | - | Chr5:46795877-46795896 | None:intergenic | 45.0% | |
| TTGGCTCCAAACTGTTGAAC+GGG | + | Chr5:46795868-46795887 | MsG0580026867.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 46795647 | 46795994 | 46795647 | ID=MsG0580026867.01;Name=MsG0580026867.01 |
| Chr5 | mRNA | 46795647 | 46795994 | 46795647 | ID=MsG0580026867.01.T01;Parent=MsG0580026867.01;Name=MsG0580026867.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|115 |
| Chr5 | exon | 46795647 | 46795994 | 46795647 | ID=MsG0580026867.01.T01:exon:18646;Parent=MsG0580026867.01.T01 |
| Chr5 | CDS | 46795647 | 46795994 | 46795647 | ID=MsG0580026867.01.T01:cds;Parent=MsG0580026867.01.T01 |
| Gene Sequence |
| Protein sequence |
