Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014947.01.T01 | PNX99352.1 | 82.609 | 92 | 15 | 1 | 1 | 91 | 1 | 92 | 1.38E-45 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014947.01.T01 | A0A2K3N8J0 | 82.609 | 92 | 15 | 1 | 1 | 91 | 1 | 92 | 6.60e-46 | 151 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000479.01 | MsG0380014947.01 | 0.812259 | 4.638808e-51 | 2.935343e-48 |
| MsG0280011460.01 | MsG0380014947.01 | 0.821973 | 3.036281e-53 | 2.507751e-50 |
| MsG0380014947.01 | MsG0380015097.01 | 0.809318 | 2.007512e-50 | 1.174901e-47 |
| MsG0380014947.01 | MsG0380015455.01 | 0.809753 | 1.619375e-50 | 9.588087e-48 |
| MsG0380014947.01 | MsG0380016348.01 | 0.838415 | 2.914466e-57 | 3.900971e-54 |
| MsG0380014947.01 | MsG0380016573.01 | 0.827255 | 1.730315e-54 | 1.661657e-51 |
| MsG0380014947.01 | MsG0480018333.01 | 0.813438 | 2.559699e-51 | 1.671625e-48 |
| MsG0380014947.01 | MsG0480019890.01 | 0.831954 | 1.245052e-55 | 1.372039e-52 |
| MsG0380014947.01 | MsG0480020547.01 | 0.834669 | 2.622902e-56 | 3.133907e-53 |
| MsG0380014947.01 | MsG0480021237.01 | 0.806431 | 8.253365e-50 | 4.478874e-47 |
| MsG0380014947.01 | MsG0480022812.01 | 0.812061 | 5.123013e-51 | 3.224135e-48 |
| MsG0380014947.01 | MsG0580025746.01 | 0.804455 | 2.142140e-49 | 1.104966e-46 |
| MsG0380014947.01 | MsG0580026867.01 | 0.801558 | 8.509313e-49 | 4.075984e-46 |
| MsG0380014947.01 | MsG0680033623.01 | 0.837915 | 3.920121e-57 | 5.167454e-54 |
| MsG0280007071.01 | MsG0380014947.01 | 0.820813 | 5.624209e-53 | 4.496814e-50 |
| MsG0280008856.01 | MsG0380014947.01 | 0.800238 | 1.583217e-48 | 7.334414e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014947.01.T01 | MTR_3g466420 | 94.048 | 84 | 4 | 1 | 1 | 84 | 1 | 83 | 8.71e-44 | 137 |
| MsG0380014947.01.T01 | MTR_5g098280 | 75.862 | 87 | 11 | 2 | 1 | 87 | 1 | 77 | 8.94e-40 | 126 |
| MsG0380014947.01.T01 | MTR_0398s0030 | 81.609 | 87 | 14 | 2 | 1 | 87 | 1 | 85 | 6.24e-36 | 117 |
| MsG0380014947.01.T01 | MTR_5g075070 | 58.696 | 92 | 34 | 3 | 1 | 89 | 1 | 91 | 8.91e-32 | 107 |
| MsG0380014947.01.T01 | MTR_6g029390 | 46.988 | 83 | 37 | 1 | 1 | 83 | 1 | 76 | 7.19e-23 | 84.3 |
| MsG0380014947.01.T01 | MTR_5g098200 | 44.578 | 83 | 39 | 1 | 1 | 83 | 1 | 76 | 7.22e-21 | 79.3 |
| MsG0380014947.01.T01 | MTR_7g085610 | 41.379 | 87 | 41 | 2 | 1 | 86 | 1 | 78 | 1.25e-15 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014947.01.T01 | AT5G58375 | 50.000 | 94 | 35 | 3 | 1 | 91 | 1 | 85 | 3.78e-22 | 82.4 |
| MsG0380014947.01.T01 | AT5G18150 | 42.169 | 83 | 33 | 2 | 1 | 83 | 1 | 68 | 2.34e-17 | 70.1 |
| MsG0380014947.01.T01 | AT5G14602 | 39.759 | 83 | 39 | 1 | 1 | 83 | 1 | 72 | 4.38e-17 | 69.3 |
Find 22 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTAACTGTTTCTGTTA+TGG | 0.220559 | 3:-62908468 | MsG0380014947.01.T01:three_prime_UTR |
| TCTTCATCATCATCGATCTT+AGG | 0.252325 | 3:+62908976 | None:intergenic |
| ATATGATTGAATCTGGGTTT+TGG | 0.273834 | 3:-62908789 | MsG0380014947.01.T01:CDS |
| GTTCGGAATATGATTGAATC+TGG | 0.288666 | 3:-62908796 | MsG0380014947.01.T01:CDS |
| TTCATCAAATGAAAATTCTA+AGG | 0.310378 | 3:-62908905 | MsG0380014947.01.T01:intron |
| TGGCTAGTGGACGTTATCTT+TGG | 0.311955 | 3:-62908753 | MsG0380014947.01.T01:CDS |
| TAGGTTGATTTCTGAAATTT+CGG | 0.333725 | 3:+62908995 | None:intergenic |
| ACGTTATCTTTGGAGGAATA+TGG | 0.339509 | 3:-62908743 | MsG0380014947.01.T01:CDS |
| ATATGTCAAAGTCAAACTTA+TGG | 0.386842 | 3:-62908497 | MsG0380014947.01.T01:three_prime_UTR |
| ATTTCGGAGAACGAAGATTC+CGG | 0.399084 | 3:+62909011 | None:intergenic |
| AAATCAAAATGTGCCCTTTG+AGG | 0.417218 | 3:-62909065 | MsG0380014947.01.T01:exon |
| AAAGAACATTTATTAAGCTT+AGG | 0.460103 | 3:-62909151 | MsG0380014947.01.T01:five_prime_UTR |
| GTCAAACTTTGATATTCTGT+TGG | 0.467478 | 3:-62908383 | MsG0380014947.01.T01:three_prime_UTR |
| CTAGTGGACGTTATCTTTGG+AGG | 0.484216 | 3:-62908750 | MsG0380014947.01.T01:CDS |
| AATATGAGAATTAGCCTCAA+AGG | 0.502029 | 3:+62909051 | None:intergenic |
| ATATGAGAATTAGCCTCAAA+GGG | 0.515144 | 3:+62909052 | None:intergenic |
| TTCGGAATATGATTGAATCT+GGG | 0.519834 | 3:-62908795 | MsG0380014947.01.T01:CDS |
| TCTAGCTGATGATGTGTACT+TGG | 0.520132 | 3:-62908700 | MsG0380014947.01.T01:exon |
| TTCTTGTCCGCAACACTCGC+CGG | 0.522394 | 3:-62909030 | MsG0380014947.01.T01:CDS |
| TGTACTTGGTGAATGCATGT+TGG | 0.535879 | 3:-62908686 | MsG0380014947.01.T01:three_prime_UTR |
| GTACTTGGTGAATGCATGTT+GGG | 0.550068 | 3:-62908685 | MsG0380014947.01.T01:three_prime_UTR |
| GAAGATTCCGGCGAGTGTTG+CGG | 0.583534 | 3:+62909023 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTAAAATTAAAGCAAAA+GGG | + | Chr3:62908746-62908765 | None:intergenic | 10.0% |
| !! | TTTATTAAAATTAAAGCAAA+AGG | + | Chr3:62908747-62908766 | None:intergenic | 10.0% |
| !! | AAAGAACATTTATTAAGCTT+AGG | - | Chr3:62908461-62908480 | MsG0380014947.01.T01:three_prime_UTR | 20.0% |
| !! | AATCAACAAATCACAAATTT+AGG | + | Chr3:62908792-62908811 | None:intergenic | 20.0% |
| !! | ATCAACAAATCACAAATTTA+GGG | + | Chr3:62908791-62908810 | None:intergenic | 20.0% |
| !! | TTCATCAAATGAAAATTCTA+AGG | - | Chr3:62908707-62908726 | MsG0380014947.01.T01:exon | 20.0% |
| !!! | AGTATTGTTGTTTTTATTGT+TGG | - | Chr3:62909035-62909054 | MsG0380014947.01.T01:CDS | 20.0% |
| ! | ATATGTCAAAGTCAAACTTA+TGG | - | Chr3:62909115-62909134 | MsG0380014947.01.T01:five_prime_UTR | 25.0% |
| !!! | ATTTGTGATTTGTTGATTCT+AGG | - | Chr3:62908794-62908813 | MsG0380014947.01.T01:CDS | 25.0% |
| !!! | TAGGTTGATTTCTGAAATTT+CGG | + | Chr3:62908620-62908639 | None:intergenic | 25.0% |
| !!! | TTCTTTAACTGTTTCTGTTA+TGG | - | Chr3:62909144-62909163 | MsG0380014947.01.T01:five_prime_UTR | 25.0% |
| !!! | TTGAAGAATCTGAGATTTTA+GGG | + | Chr3:62908683-62908702 | None:intergenic | 25.0% |
| !!! | TTTGAAGAATCTGAGATTTT+AGG | + | Chr3:62908684-62908703 | None:intergenic | 25.0% |
| ATTAAAGCAAAAGGGTCAAA+TGG | + | Chr3:62908738-62908757 | None:intergenic | 30.0% | |
| GCGAAAAATCACAATATCAT+AGG | + | Chr3:62908973-62908992 | None:intergenic | 30.0% | |
| GTCAAACTTTGATATTCTGT+TGG | - | Chr3:62909229-62909248 | MsG0380014947.01.T01:five_prime_UTR | 30.0% | |
| TTAAAGCAAAAGGGTCAAAT+GGG | + | Chr3:62908737-62908756 | None:intergenic | 30.0% | |
| TTCGGAATATGATTGAATCT+GGG | - | Chr3:62908817-62908836 | MsG0380014947.01.T01:intron | 30.0% | |
| ! | AATATGAGAATTAGCCTCAA+AGG | + | Chr3:62908564-62908583 | None:intergenic | 30.0% |
| ! | ATATGAGAATTAGCCTCAAA+GGG | + | Chr3:62908563-62908582 | None:intergenic | 30.0% |
| ! | ATATGATTGAATCTGGGTTT+TGG | - | Chr3:62908823-62908842 | MsG0380014947.01.T01:intron | 30.0% |
| !! | TGATTTGTTGATTCTAGGTT+CGG | - | Chr3:62908799-62908818 | MsG0380014947.01.T01:CDS | 30.0% |
| AAATCAAAATGTGCCCTTTG+AGG | - | Chr3:62908547-62908566 | MsG0380014947.01.T01:three_prime_UTR | 35.0% | |
| GTTCGGAATATGATTGAATC+TGG | - | Chr3:62908816-62908835 | MsG0380014947.01.T01:intron | 35.0% | |
| TCTTCATCATCATCGATCTT+AGG | + | Chr3:62908639-62908658 | None:intergenic | 35.0% | |
| ! | ACGTTATCTTTGGAGGAATA+TGG | - | Chr3:62908869-62908888 | MsG0380014947.01.T01:intron | 35.0% |
| !! | GTTTTGGACTTTTGTGGATA+TGG | - | Chr3:62908839-62908858 | MsG0380014947.01.T01:intron | 35.0% |
| !!! | TCTGAGATTTTAGGGTTAGT+AGG | + | Chr3:62908675-62908694 | None:intergenic | 35.0% |
| ATTTCGGAGAACGAAGATTC+CGG | + | Chr3:62908604-62908623 | None:intergenic | 40.0% | |
| ! | ACTTTTGTGGATATGGCTAG+TGG | - | Chr3:62908846-62908865 | MsG0380014947.01.T01:intron | 40.0% |
| ! | GTACTTGGTGAATGCATGTT+GGG | - | Chr3:62908927-62908946 | MsG0380014947.01.T01:CDS | 40.0% |
| ! | TCTAGCTGATGATGTGTACT+TGG | - | Chr3:62908912-62908931 | MsG0380014947.01.T01:CDS | 40.0% |
| ! | TGTACTTGGTGAATGCATGT+TGG | - | Chr3:62908926-62908945 | MsG0380014947.01.T01:CDS | 40.0% |
| !!! | ATCTGGGTTTTGGACTTTTG+TGG | - | Chr3:62908833-62908852 | MsG0380014947.01.T01:intron | 40.0% |
| ! | CTAGTGGACGTTATCTTTGG+AGG | - | Chr3:62908862-62908881 | MsG0380014947.01.T01:intron | 45.0% |
| ! | TGGCTAGTGGACGTTATCTT+TGG | - | Chr3:62908859-62908878 | MsG0380014947.01.T01:intron | 45.0% |
| TTCTTGTCCGCAACACTCGC+CGG | - | Chr3:62908582-62908601 | MsG0380014947.01.T01:three_prime_UTR | 55.0% | |
| ! | GAAGATTCCGGCGAGTGTTG+CGG | + | Chr3:62908592-62908611 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 62908383 | 62909251 | 62908383 | ID=MsG0380014947.01;Name=MsG0380014947.01 |
| Chr3 | mRNA | 62908383 | 62909251 | 62908383 | ID=MsG0380014947.01.T01;Parent=MsG0380014947.01;Name=MsG0380014947.01.T01;_AED=0.49;_eAED=0.49;_QI=172|1|1|1|1|1|2|331|92 |
| Chr3 | exon | 62908383 | 62908818 | 62908383 | ID=MsG0380014947.01.T01:exon:8529;Parent=MsG0380014947.01.T01 |
| Chr3 | exon | 62908906 | 62909251 | 62908906 | ID=MsG0380014947.01.T01:exon:8528;Parent=MsG0380014947.01.T01 |
| Chr3 | five_prime_UTR | 62909080 | 62909251 | 62909080 | ID=MsG0380014947.01.T01:five_prime_utr;Parent=MsG0380014947.01.T01 |
| Chr3 | CDS | 62908906 | 62909079 | 62908906 | ID=MsG0380014947.01.T01:cds;Parent=MsG0380014947.01.T01 |
| Chr3 | CDS | 62908714 | 62908818 | 62908714 | ID=MsG0380014947.01.T01:cds;Parent=MsG0380014947.01.T01 |
| Chr3 | three_prime_UTR | 62908383 | 62908713 | 62908383 | ID=MsG0380014947.01.T01:three_prime_utr;Parent=MsG0380014947.01.T01 |
| Gene Sequence |
| Protein sequence |
