AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027083.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027083.01.T01 MTR_0194s0040 79.021 286 5 6 1 283 1 234 6.32e-148 414
MsG0580027083.01.T01 MTR_4g129850 56.502 223 63 3 56 276 26 216 2.66e-81 244
MsG0580027083.01.T01 MTR_4g129860 55.760 217 62 3 62 276 33 217 6.84e-77 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027083.01.T01 AT1G26160 62.132 272 62 6 15 278 13 251 2.38e-107 312
MsG0580027083.01.T01 AT2G23820 58.850 226 61 2 52 277 61 254 3.05e-81 246
MsG0580027083.01.T01 AT1G26160 55.752 226 59 6 15 232 13 205 5.82e-73 223
MsG0580027083.01.T01 AT2G23820 57.416 209 57 2 52 260 61 237 1.19e-69 216

Find 82 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAGAGGGTTCATGTTTC+TGG 0.050788 5:+52881214 None:intergenic
GGGAAGTTTCAAACTGAAAT+AGG 0.229767 5:-52878224 MsG0580027083.01.T01:CDS
AACAAAATGTGTCAAGTTCT+TGG 0.241345 5:-52879603 MsG0580027083.01.T01:CDS
CATACTCCAACCACAGTTCT+TGG 0.247596 5:+52879462 None:intergenic
AGGGTTCATGTTTCTGGGTC+TGG 0.250821 5:+52881220 None:intergenic
TGAAAACAATTCTTCTTTAG+AGG 0.265718 5:-52879441 MsG0580027083.01.T01:CDS
AACCCATCCTTTCCTCTTTG+TGG 0.273114 5:+52881021 None:intergenic
AAAATTCTACAACCAAGTTC+TGG 0.302414 5:+52878042 None:intergenic
TAACTACGCTCCTCTCGTTT+TGG 0.306435 5:-52881250 MsG0580027083.01.T01:CDS
AAGTATTTGACAATAATTTA+CGG 0.338795 5:+52877872 None:intergenic
TGTTGTTGTCCCTGTCTAAT+AGG 0.352661 5:-52877991 MsG0580027083.01.T01:three_prime_UTR
CAATTAGACCTGGTCTGGTT+TGG 0.355726 5:-52878067 MsG0580027083.01.T01:three_prime_UTR
TGGTGCCACATTGCTCATTC+TGG 0.356906 5:-52878122 MsG0580027083.01.T01:three_prime_UTR
ACAAGCACTGGAATATGAAA+TGG 0.360466 5:-52878591 MsG0580027083.01.T01:CDS
TGGGTTAATCATGGGATTAA+AGG 0.371640 5:-52881004 MsG0580027083.01.T01:CDS
GTGAGAGGGTTCATGTTTCT+GGG 0.373758 5:+52881215 None:intergenic
TCATTCTGGTCGGTGAGATT+TGG 0.382607 5:-52878108 MsG0580027083.01.T01:three_prime_UTR
GGGTTCATGTTTCTGGGTCT+GGG 0.384724 5:+52881221 None:intergenic
GCCAACAATTAGACCTGGTC+TGG 0.397169 5:-52878072 MsG0580027083.01.T01:three_prime_UTR
AAACTGAAATAGGTAAAAGT+TGG 0.400138 5:-52878214 MsG0580027083.01.T01:CDS
CTCATTGCTTCTGATGTCCC+TGG 0.410575 5:-52880935 MsG0580027083.01.T01:CDS
TTATACTCAATAGCTATTGT+AGG 0.435237 5:-52879690 MsG0580027083.01.T01:CDS
GTAAATTATTGTCAAATACT+TGG 0.435240 5:-52877870 MsG0580027083.01.T01:three_prime_UTR
GGGTTAATCATGGGATTAAA+GGG 0.440585 5:-52881003 MsG0580027083.01.T01:CDS
TTAAAGCTTCCTGCTCCATT+CGG 0.449281 5:+52879625 None:intergenic
AAACGAGAGGAGCGTAGTTA+CGG 0.451636 5:+52881253 None:intergenic
AGCAGAAGAGGAGGAGGAGG+AGG 0.452726 5:+52881167 None:intergenic
TCTGGTTTGGATCCAGAACT+TGG 0.458729 5:-52878054 MsG0580027083.01.T01:three_prime_UTR
GGAGGAGGAGGAGGTGTGAG+AGG 0.460553 5:+52881200 None:intergenic
AGGAAAGGATGGGTTAATCA+TGG 0.468444 5:-52881013 MsG0580027083.01.T01:CDS
TCAAAGCCATACGGTACATA+TGG 0.474924 5:+52880963 None:intergenic
AACTGAAATAGGTAAAAGTT+GGG 0.477183 5:-52878213 MsG0580027083.01.T01:CDS
TGAAATGATTCTACAAGCAC+TGG 0.492894 5:-52878603 MsG0580027083.01.T01:CDS
TGAAAAGAGCCGAATGGAGC+AGG 0.493928 5:-52879634 MsG0580027083.01.T01:CDS
CAAGCACTGGAATATGAAAT+GGG 0.501459 5:-52878590 MsG0580027083.01.T01:intron
ACCAGACCAGGTCTAATTGT+TGG 0.501689 5:+52878071 None:intergenic
AACCTTTCTCTACTCAATCC+AGG 0.503336 5:+52880917 None:intergenic
ATCGATAGCAGAAGAGGAGG+AGG 0.510416 5:+52881161 None:intergenic
ACATACCTTCTGCAATATCA+TGG 0.513774 5:+52880795 None:intergenic
TTCTGGATCCAAACCAGACC+AGG 0.516430 5:+52878059 None:intergenic
GAGGAGGAGGAGGTGTGAGA+GGG 0.516567 5:+52881201 None:intergenic
TAAATTATTGTCAAATACTT+GGG 0.518094 5:-52877869 MsG0580027083.01.T01:three_prime_UTR
ATGTGTCAAGTTCTTGGTGG+AGG 0.521274 5:-52879597 MsG0580027083.01.T01:CDS
TTAATAATTACGCAAGCAGT+CGG 0.524958 5:-52881347 MsG0580027083.01.T01:CDS
GAGAAAATCGATAGCAGAAG+AGG 0.527137 5:+52881155 None:intergenic
GATAGCAGAAGAGGAGGAGG+AGG 0.532295 5:+52881164 None:intergenic
ACCATCTGATGGTGTGCCTA+AGG 0.532689 5:-52879658 MsG0580027083.01.T01:CDS
AATTGCACAACTTTCAACTC+TGG 0.532940 5:+52877813 None:intergenic
GAGAGCATACCTTGAGAGTG+TGG 0.537102 5:+52881124 None:intergenic
ACGGTACATATGGTCAGCTA+TGG 0.543472 5:+52880973 None:intergenic
GTCAAATTTAAATGCGTCAA+CGG 0.548197 5:+52877929 None:intergenic
GTTGATAAAATGGCTAAAAG+TGG 0.550713 5:-52878142 MsG0580027083.01.T01:exon
GGAAAGGATGGGTTAATCAT+GGG 0.554187 5:-52881012 MsG0580027083.01.T01:CDS
TAGTTACGGAGAACAACAAA+GGG 0.564744 5:+52881267 None:intergenic
CACCACTGCATCATCATCAT+CGG 0.569833 5:-52881314 MsG0580027083.01.T01:CDS
AAAATGTGTCAAGTTCTTGG+TGG 0.570688 5:-52879600 MsG0580027083.01.T01:CDS
AGCTGACCATATGTACCGTA+TGG 0.583476 5:-52880969 MsG0580027083.01.T01:CDS
TCCCTGGATTGAGTAGAGAA+AGG 0.585181 5:-52880919 MsG0580027083.01.T01:CDS
CACTCTCTGCCACACTCTCA+AGG 0.588274 5:-52881133 MsG0580027083.01.T01:intron
AAAATCGATAGCAGAAGAGG+AGG 0.590653 5:+52881158 None:intergenic
CTCGTCCATGATATTGCAGA+AGG 0.591615 5:-52880800 MsG0580027083.01.T01:intron
GTTCTTGGTGGAGGGATGAG+AGG 0.593011 5:-52879588 MsG0580027083.01.T01:intron
AGACCACAAAGAGGAAAGGA+TGG 0.598959 5:-52881024 MsG0580027083.01.T01:CDS
CACAGCACAGAAGAAAGCAA+AGG 0.603852 5:+52880842 None:intergenic
GTGTAGACCACAAAGAGGAA+AGG 0.612615 5:-52881028 MsG0580027083.01.T01:intron
ACCTTTCTCTACTCAATCCA+GGG 0.616102 5:+52880918 None:intergenic
GCCTTAGGCACACCATCAGA+TGG 0.628508 5:+52879657 None:intergenic
TGAGATCCAAGAACTGTGGT+TGG 0.629105 5:-52879468 MsG0580027083.01.T01:CDS
GTAGTTACGGAGAACAACAA+AGG 0.638371 5:+52881266 None:intergenic
TAAGGCTGAAAAGAGCCGAA+TGG 0.640673 5:-52879640 MsG0580027083.01.T01:CDS
GCCACATTGCTCATTCTGGT+CGG 0.640875 5:-52878118 MsG0580027083.01.T01:three_prime_UTR
GGAGATATAACACCATCTGA+TGG 0.641949 5:-52879669 MsG0580027083.01.T01:CDS
CGCCGATGATGATGATGCAG+TGG 0.642588 5:+52881312 None:intergenic
TTATTGCCAACAATTAGACC+TGG 0.642757 5:-52878077 MsG0580027083.01.T01:three_prime_UTR
GACCACAAAGAGGAAAGGAT+GGG 0.643435 5:-52881023 MsG0580027083.01.T01:CDS
TGTGTCAAGTTCTTGGTGGA+GGG 0.662562 5:-52879596 MsG0580027083.01.T01:CDS
TGAGTGACATCAAAGCCATA+CGG 0.663318 5:+52880954 None:intergenic
GGTTAATCATGGGATTAAAG+GGG 0.672063 5:-52881002 MsG0580027083.01.T01:CDS
GTATTGTGTAGACCACAAAG+AGG 0.700403 5:-52881033 MsG0580027083.01.T01:intron
TGGGAACGAACCAAAACGAG+AGG 0.703050 5:+52881240 None:intergenic
CTGATGAGATCCAAGAACTG+TGG 0.731755 5:-52879472 MsG0580027083.01.T01:CDS
ACCGACCAGAATGAGCAATG+TGG 0.752818 5:+52878117 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATATAATTAAAAAGAAA+AGG - Chr5:52877821-52877840 MsG0580027083.01.T01:three_prime_UTR 10.0%
!! TCTTTCATAATATAAATTAA+AGG + Chr5:52880675-52880694 None:intergenic 10.0%
!!! TAGAATTTTAAACAATATTT+GGG - Chr5:52881177-52881196 MsG0580027083.01.T01:CDS 10.0%
!! AAATATATTGAAAAAAACGA+CGG + Chr5:52879461-52879480 None:intergenic 15.0%
!! AAGTATTTGACAATAATTTA+CGG + Chr5:52881336-52881355 None:intergenic 15.0%
!! AGATAAAACAACATTAAAAA+AGG + Chr5:52881250-52881269 None:intergenic 15.0%
!! AGTTATTTAGTATTAAAGAA+CGG + Chr5:52880807-52880826 None:intergenic 15.0%
!! ATAAAACAACATTAAAAAAG+GGG + Chr5:52881248-52881267 None:intergenic 15.0%
!! CTTTCATAATATAAATTCAA+AGG + Chr5:52880712-52880731 None:intergenic 15.0%
!! GAAAAAAAGAAAATATGAAA+CGG + Chr5:52881305-52881324 None:intergenic 15.0%
!! GATAAAACAACATTAAAAAA+GGG + Chr5:52881249-52881268 None:intergenic 15.0%
!! TAAATTATTGTCAAATACTT+GGG - Chr5:52881336-52881355 MsG0580027083.01.T01:CDS 15.0%
!!! AAAAAGTTAAGTTGATAAAA+TGG - Chr5:52881053-52881072 MsG0580027083.01.T01:intron 15.0%
!!! AATTTTTTGTTTTGTATTCA+GGG - Chr5:52880961-52880980 MsG0580027083.01.T01:CDS 15.0%
!!! CAAATCAGAATATATTTTTA+TGG - Chr5:52880390-52880409 MsG0580027083.01.T01:intron 15.0%
!!! GTAGAATTTTAAACAATATT+TGG - Chr5:52881176-52881195 MsG0580027083.01.T01:CDS 15.0%
!!! TAATTTTTTGTTTTGTATTC+AGG - Chr5:52880960-52880979 MsG0580027083.01.T01:CDS 15.0%
!! AAAAAGAAAAGGACAAAAAA+TGG - Chr5:52877832-52877851 MsG0580027083.01.T01:three_prime_UTR 20.0%
!! CAATAAAGATAAAAGGAATT+TGG - Chr5:52878846-52878865 MsG0580027083.01.T01:intron 20.0%
!! CAATTCAATTCAATTACATA+AGG + Chr5:52878137-52878156 None:intergenic 20.0%
!! GTAAATTATTGTCAAATACT+TGG - Chr5:52881335-52881354 MsG0580027083.01.T01:CDS 20.0%
!! TAAAACAACATTAAAAAAGG+GGG + Chr5:52881247-52881266 None:intergenic 20.0%
!!! AATTTGGAACATTAAAAACT+TGG - Chr5:52879998-52880017 MsG0580027083.01.T01:intron 20.0%
!!! ATTACATTACATTTTCTCTT+CGG - Chr5:52878550-52878569 MsG0580027083.01.T01:intron 20.0%
!!! CTGAAATTTTTGCTTATAAT+AGG + Chr5:52878598-52878617 None:intergenic 20.0%
!!! TGATTTTGAAGATAATATGA+AGG - Chr5:52880023-52880042 MsG0580027083.01.T01:intron 20.0%
!!! TTTGTCACTAAATTTTGATT+TGG + Chr5:52878730-52878749 None:intergenic 20.0%
!!! TTTTCTTCTTGAAATGATTT+CGG + Chr5:52881021-52881040 None:intergenic 20.0%
! AAAAAAATGATGATACGCAT+GGG + Chr5:52880351-52880370 None:intergenic 25.0%
! AAAAATTATGTGACTGACAA+AGG + Chr5:52880948-52880967 None:intergenic 25.0%
! AAACTGAAATAGGTAAAAGT+TGG - Chr5:52880991-52881010 MsG0580027083.01.T01:CDS 25.0%
! AAATGTAATGTAATGCAATC+AGG + Chr5:52878544-52878563 None:intergenic 25.0%
! AACCATTAGAAAAGATTTAG+AGG + Chr5:52879002-52879021 None:intergenic 25.0%
! AACTGAAATAGGTAAAAGTT+GGG - Chr5:52880992-52881011 MsG0580027083.01.T01:CDS 25.0%
! AGGTCTACAATAAAGATAAA+AGG - Chr5:52878839-52878858 MsG0580027083.01.T01:intron 25.0%
! ATGAATCTGAAAAAAGAGAT+TGG - Chr5:52878677-52878696 MsG0580027083.01.T01:intron 25.0%
! ATTTCAGATTCATTGAATCA+CGG - Chr5:52878611-52878630 MsG0580027083.01.T01:intron 25.0%
! TACAAGTTATAAACACTATC+CGG + Chr5:52878704-52878723 None:intergenic 25.0%
! TCAATTACATAAGGAATGAA+TGG + Chr5:52878128-52878147 None:intergenic 25.0%
! TGAAAACAATTCTTCTTTAG+AGG - Chr5:52879764-52879783 MsG0580027083.01.T01:intron 25.0%
! TTATACTCAATAGCTATTGT+AGG - Chr5:52879515-52879534 MsG0580027083.01.T01:intron 25.0%
!! GTTTCTCTAATAGTTTTCTT+AGG - Chr5:52879019-52879038 MsG0580027083.01.T01:intron 25.0%
!! TGACTAAATGATTGATTCTA+GGG + Chr5:52880169-52880188 None:intergenic 25.0%
!!! TAGAAAAGTTGGTAGAAAAT+AGG + Chr5:52879144-52879163 None:intergenic 25.0%
!!! TGTTGTTAAAGATTTTGACA+AGG - Chr5:52879791-52879810 MsG0580027083.01.T01:intron 25.0%
!!! TTTGAGATGACATTCTTTTT+TGG - Chr5:52879701-52879720 MsG0580027083.01.T01:intron 25.0%
AAAATTCTACAACCAAGTTC+TGG + Chr5:52881166-52881185 None:intergenic 30.0%
AACAAAATGTGTCAAGTTCT+TGG - Chr5:52879602-52879621 MsG0580027083.01.T01:CDS 30.0%
AACAGAGAAAGGTTTAATGA+AGG + Chr5:52879872-52879891 None:intergenic 30.0%
AATTTACTTTGAGTCCAAGA+TGG + Chr5:52879897-52879916 None:intergenic 30.0%
ACATAAAAGAAAGCAAGCAT+GGG + Chr5:52880199-52880218 None:intergenic 30.0%
AGATAAAAGGAATTTGGCTA+AGG - Chr5:52878852-52878871 MsG0580027083.01.T01:intron 30.0%
ATGATAACTAATGCTACAAG+AGG + Chr5:52879932-52879951 None:intergenic 30.0%
CAATAGGGATTCATGTTTAA+TGG - Chr5:52880224-52880243 MsG0580027083.01.T01:intron 30.0%
CATTAAACATGAATCCCTAT+TGG + Chr5:52880226-52880245 None:intergenic 30.0%
CTTGATTATATTGTACCTTG+TGG - Chr5:52880825-52880844 MsG0580027083.01.T01:CDS 30.0%
GAAAAAAATGATGATACGCA+TGG + Chr5:52880352-52880371 None:intergenic 30.0%
GAAATGCATGAATCCTTATT+TGG - Chr5:52880921-52880940 MsG0580027083.01.T01:CDS 30.0%
GAGGAAACAAAACGTAATAA+AGG + Chr5:52880471-52880490 None:intergenic 30.0%
GTAGTATAAACCAGATACAT+TGG - Chr5:52878647-52878666 MsG0580027083.01.T01:intron 30.0%
GTCAAATTTAAATGCGTCAA+CGG + Chr5:52881279-52881298 None:intergenic 30.0%
TAGAACTGTAATATGGAGAT+TGG - Chr5:52880505-52880524 MsG0580027083.01.T01:intron 30.0%
TCTTTGATAGAACATTATGG+CGG + Chr5:52878955-52878974 None:intergenic 30.0%
TGATAACTAATGCTACAAGA+GGG + Chr5:52879931-52879950 None:intergenic 30.0%
TGGTCTTTGATAGAACATTA+TGG + Chr5:52878958-52878977 None:intergenic 30.0%
TTAATAATTACGCAAGCAGT+CGG - Chr5:52877858-52877877 MsG0580027083.01.T01:three_prime_UTR 30.0%
TTACATAAGGAATGAATGGA+TGG + Chr5:52878124-52878143 None:intergenic 30.0%
TTTATTGTAGACCTATACAC+CGG + Chr5:52878833-52878852 None:intergenic 30.0%
! CAATGAGTTGGATCTAAATA+TGG + Chr5:52878978-52878997 None:intergenic 30.0%
! CTACGATAATAGATTTTCGT+TGG + Chr5:52879968-52879987 None:intergenic 30.0%
! GAATTTTTCATTTCAACTGC+AGG - Chr5:52880871-52880890 MsG0580027083.01.T01:CDS 30.0%
! GACCTCTAAATCTTTTCTAA+TGG - Chr5:52878997-52879016 MsG0580027083.01.T01:intron 30.0%
! GTGACTAAATGATTGATTCT+AGG + Chr5:52880170-52880189 None:intergenic 30.0%
! GTTGATAAAATGGCTAAAAG+TGG - Chr5:52881063-52881082 MsG0580027083.01.T01:intron 30.0%
! TCGTAGATGTATCTGAAATT+TGG - Chr5:52879982-52880001 MsG0580027083.01.T01:intron 30.0%
! TCTACCAACTTTTCTACTAT+AGG - Chr5:52879148-52879167 MsG0580027083.01.T01:intron 30.0%
! TCTTTTATGTGTAGCCAATA+GGG - Chr5:52880209-52880228 MsG0580027083.01.T01:intron 30.0%
! TTCTTTTATGTGTAGCCAAT+AGG - Chr5:52880208-52880227 MsG0580027083.01.T01:intron 30.0%
!! AATTGCATTGTTTTACCTTC+TGG - Chr5:52880548-52880567 MsG0580027083.01.T01:intron 30.0%
!! GCATCTTTAGAACTGTAATA+TGG - Chr5:52880498-52880517 MsG0580027083.01.T01:intron 30.0%
!!! CTAATTGCATTGTTTTGATC+AGG - Chr5:52880578-52880597 MsG0580027083.01.T01:intron 30.0%
AAAATGTGTCAAGTTCTTGG+TGG - Chr5:52879605-52879624 MsG0580027083.01.T01:CDS 35.0%
AACTTTCCTTTCAGCTTAAG+TGG - Chr5:52879346-52879365 MsG0580027083.01.T01:intron 35.0%
AAGAAGAGTAGATCAAATGG+CGG + Chr5:52879074-52879093 None:intergenic 35.0%
AATGAATGGATGGATCTTTG+TGG + Chr5:52878114-52878133 None:intergenic 35.0%
ACATACCTTCTGCAATATCA+TGG + Chr5:52878413-52878432 None:intergenic 35.0%
AGACTAGACATGATAGGATT+AGG + Chr5:52879207-52879226 None:intergenic 35.0%
AGATTTAGAGGTCAATGAGT+TGG + Chr5:52878990-52879009 None:intergenic 35.0%
AGCACCTATAGTAGAAAAGT+TGG + Chr5:52879155-52879174 None:intergenic 35.0%
AGCTGAAAGGAAAGTTCTAT+CGG + Chr5:52879342-52879361 None:intergenic 35.0%
CAAGGCAGTAATTAGGAAAA+TGG - Chr5:52880088-52880107 MsG0580027083.01.T01:intron 35.0%
CACATAAAAGAAAGCAAGCA+TGG + Chr5:52880200-52880219 None:intergenic 35.0%
CATCGAATAACCAATGTATC+TGG + Chr5:52878660-52878679 None:intergenic 35.0%
GAAAATAGGCTTAGATGGTT+TGG + Chr5:52879130-52879149 None:intergenic 35.0%
GGCAAAAATCCTATTAGACA+GGG + Chr5:52881226-52881245 None:intergenic 35.0%
GGGAAGTTTCAAACTGAAAT+AGG - Chr5:52880981-52881000 MsG0580027083.01.T01:CDS 35.0%
GGGTTAATCATGGGATTAAA+GGG - Chr5:52878202-52878221 MsG0580027083.01.T01:CDS 35.0%
GGTAAGAAGAGTAGATCAAA+TGG + Chr5:52879077-52879096 None:intergenic 35.0%
GGTTAATCATGGGATTAAAG+GGG - Chr5:52878203-52878222 MsG0580027083.01.T01:CDS 35.0%
TACATCATCGTATTCAAGCT+GGG + Chr5:52879420-52879439 None:intergenic 35.0%
TAGTTACGGAGAACAACAAA+GGG + Chr5:52877941-52877960 None:intergenic 35.0%
TCTGAAAAAAGAGATTGGAC+CGG - Chr5:52878682-52878701 MsG0580027083.01.T01:intron 35.0%
TGAAATGATTCTACAAGCAC+TGG - Chr5:52880602-52880621 MsG0580027083.01.T01:intron 35.0%
TGGGTTAATCATGGGATTAA+AGG - Chr5:52878201-52878220 MsG0580027083.01.T01:CDS 35.0%
TGGTAGAAAATAGGCTTAGA+TGG + Chr5:52879135-52879154 None:intergenic 35.0%
TTATTGCCAACAATTAGACC+TGG - Chr5:52881128-52881147 MsG0580027083.01.T01:intron 35.0%
TTGATCTACTCTTCTTACCA+CGG - Chr5:52879078-52879097 MsG0580027083.01.T01:intron 35.0%
! AAAGTACCACTTAAGCTGAA+AGG + Chr5:52879355-52879374 None:intergenic 35.0%
! AACGATTATCAAAGCACTTC+AGG + Chr5:52878514-52878533 None:intergenic 35.0%
! ATTATCAAAGCACTTCAGGT+AGG + Chr5:52878510-52878529 None:intergenic 35.0%
! CATTGACAAGCTTTTATTGC+TGG + Chr5:52880292-52880311 None:intergenic 35.0%
! GATTGATTCTAGGGAAGTTT+AGG + Chr5:52880160-52880179 None:intergenic 35.0%
! TTCTAAAGATGCATGTTACG+AGG + Chr5:52880490-52880509 None:intergenic 35.0%
! TTTTCCTAATTACTGCCTTG+AGG + Chr5:52880088-52880107 None:intergenic 35.0%
!! ACAAGCACTGGAATATGAAA+TGG - Chr5:52880614-52880633 MsG0580027083.01.T01:intron 35.0%
!! CAAGCACTGGAATATGAAAT+GGG - Chr5:52880615-52880634 MsG0580027083.01.T01:intron 35.0%
!! TTCCTCAGAGCTTTGAAATT+TGG - Chr5:52879667-52879686 MsG0580027083.01.T01:CDS 35.0%
!!! ACTCAACACATTGCTTTTTC+CGG - Chr5:52878811-52878830 MsG0580027083.01.T01:intron 35.0%
AAAATCGATAGCAGAAGAGG+AGG + Chr5:52878050-52878069 None:intergenic 40.0%
AACCTTTCTCTACTCAATCC+AGG + Chr5:52878291-52878310 None:intergenic 40.0%
ACATCATTAGAGAGAGAGTG+GGG + Chr5:52879180-52879199 None:intergenic 40.0%
ACCTTTCTCTACTCAATCCA+GGG + Chr5:52878290-52878309 None:intergenic 40.0%
AGACAACTAAGCAACAGTTC+AGG + Chr5:52878778-52878797 None:intergenic 40.0%
AGTCAGGTTAAAAGAGGTAG+AGG + Chr5:52879049-52879068 None:intergenic 40.0%
CCAAACAGTCTTCATGTTGA+GGG - Chr5:52880319-52880338 MsG0580027083.01.T01:intron 40.0%
CCAAGATGGTAAACAGAGAA+AGG + Chr5:52879883-52879902 None:intergenic 40.0%
CCTCAACATGAAGACTGTTT+GGG + Chr5:52880321-52880340 None:intergenic 40.0%
CCTTTCTCTGTTTACCATCT+TGG - Chr5:52879880-52879899 MsG0580027083.01.T01:intron 40.0%
GACATCATTAGAGAGAGAGT+GGG + Chr5:52879181-52879200 None:intergenic 40.0%
GACTGACAAAGGTCCAAATA+AGG + Chr5:52880937-52880956 None:intergenic 40.0%
GAGAAAATCGATAGCAGAAG+AGG + Chr5:52878053-52878072 None:intergenic 40.0%
GCAATTAGAAAGCAACCAGA+AGG + Chr5:52880534-52880553 None:intergenic 40.0%
GCTTATAATAGGTGACCAGA+GGG + Chr5:52878587-52878606 None:intergenic 40.0%
GGAGATATAACACCATCTGA+TGG - Chr5:52879536-52879555 MsG0580027083.01.T01:intron 40.0%
GGGCAAAAATCCTATTAGAC+AGG + Chr5:52881227-52881246 None:intergenic 40.0%
GTACATCATCGTATTCAAGC+TGG + Chr5:52879421-52879440 None:intergenic 40.0%
GTAGTTACGGAGAACAACAA+AGG + Chr5:52877942-52877961 None:intergenic 40.0%
GTATTGTGTAGACCACAAAG+AGG - Chr5:52878172-52878191 MsG0580027083.01.T01:CDS 40.0%
GTGGTAAGACTAGACATGAT+AGG + Chr5:52879213-52879232 None:intergenic 40.0%
TCAAAGCCATACGGTACATA+TGG + Chr5:52878245-52878264 None:intergenic 40.0%
TCAGTACTATCACCTCTGAA+AGG - Chr5:52880054-52880073 MsG0580027083.01.T01:intron 40.0%
TGACATCATTAGAGAGAGAG+TGG + Chr5:52879182-52879201 None:intergenic 40.0%
TGAGTGACATCAAAGCCATA+CGG + Chr5:52878254-52878273 None:intergenic 40.0%
TGCCAAATTTCAAAGCTCTG+AGG + Chr5:52879672-52879691 None:intergenic 40.0%
TGCTTATAATAGGTGACCAG+AGG + Chr5:52878588-52878607 None:intergenic 40.0%
TGTTGTTGTCCCTGTCTAAT+AGG - Chr5:52881214-52881233 MsG0580027083.01.T01:CDS 40.0%
TTAAAGCTTCCTGCTCCATT+CGG + Chr5:52879583-52879602 None:intergenic 40.0%
!! AGGAAAGGATGGGTTAATCA+TGG - Chr5:52878192-52878211 MsG0580027083.01.T01:CDS 40.0%
!! GGAAAGGATGGGTTAATCAT+GGG - Chr5:52878193-52878212 MsG0580027083.01.T01:CDS 40.0%
!!! CATTGCTTTTTCCGGTGTAT+AGG - Chr5:52878819-52878838 MsG0580027083.01.T01:intron 40.0%
AACCCATCCTTTCCTCTTTG+TGG + Chr5:52878187-52878206 None:intergenic 45.0%
AACGCCTTATCCCACTAAGT+GGG - Chr5:52878908-52878927 MsG0580027083.01.T01:intron 45.0%
ACCAGACCAGGTCTAATTGT+TGG + Chr5:52881137-52881156 None:intergenic 45.0%
ACCGGCAATGTTGTATGGTA+CGG + Chr5:52879308-52879327 None:intergenic 45.0%
ACGGTACATATGGTCAGCTA+TGG + Chr5:52878235-52878254 None:intergenic 45.0%
AGACCACAAAGAGGAAAGGA+TGG - Chr5:52878181-52878200 MsG0580027083.01.T01:CDS 45.0%
AGCTGACCATATGTACCGTA+TGG - Chr5:52878236-52878255 MsG0580027083.01.T01:intron 45.0%
ATCATCGTATTCAAGCTGGG+TGG + Chr5:52879417-52879436 None:intergenic 45.0%
ATCCATCTGCATTGCGTACT+AGG + Chr5:52879830-52879849 None:intergenic 45.0%
ATGTGTCAAGTTCTTGGTGG+AGG - Chr5:52879608-52879627 MsG0580027083.01.T01:CDS 45.0%
CAATTAGACCTGGTCTGGTT+TGG - Chr5:52881138-52881157 MsG0580027083.01.T01:CDS 45.0%
CACAGCACAGAAGAAAGCAA+AGG + Chr5:52878366-52878385 None:intergenic 45.0%
CACCACTGCATCATCATCAT+CGG - Chr5:52877891-52877910 MsG0580027083.01.T01:three_prime_UTR 45.0%
CATACTCCAACCACAGTTCT+TGG + Chr5:52879746-52879765 None:intergenic 45.0%
CATCATTAGAGAGAGAGTGG+GGG + Chr5:52879179-52879198 None:intergenic 45.0%
CCCAAACAGTCTTCATGTTG+AGG - Chr5:52880318-52880337 MsG0580027083.01.T01:intron 45.0%
CCCTCAACATGAAGACTGTT+TGG + Chr5:52880322-52880341 None:intergenic 45.0%
CGAGAGTAAAGTAAGCGTAG+CGG + Chr5:52879260-52879279 None:intergenic 45.0%
CGCTTACTTTACTCTCGTGT+TGG - Chr5:52879263-52879282 MsG0580027083.01.T01:intron 45.0%
CTAATTACTGCCTTGAGGAC+AGG + Chr5:52880083-52880102 None:intergenic 45.0%
CTCGTCCATGATATTGCAGA+AGG - Chr5:52878405-52878424 MsG0580027083.01.T01:intron 45.0%
CTCTTCCAAAGTGACGTTGT+TGG - Chr5:52880126-52880145 MsG0580027083.01.T01:intron 45.0%
CTGATGAGATCCAAGAACTG+TGG - Chr5:52879733-52879752 MsG0580027083.01.T01:intron 45.0%
CTGTCCTCAAGGCAGTAATT+AGG - Chr5:52880081-52880100 MsG0580027083.01.T01:intron 45.0%
CTTATCGCACAAAACACCTG+AGG - Chr5:52879375-52879394 MsG0580027083.01.T01:intron 45.0%
GACCACAAAGAGGAAAGGAT+GGG - Chr5:52878182-52878201 MsG0580027083.01.T01:CDS 45.0%
GGTTTGGACATGTAGAGAGA+AGG + Chr5:52879114-52879133 None:intergenic 45.0%
GTGAGAGGGTTCATGTTTCT+GGG + Chr5:52877993-52878012 None:intergenic 45.0%
GTGTAGACCACAAAGAGGAA+AGG - Chr5:52878177-52878196 MsG0580027083.01.T01:CDS 45.0%
TAAGGCTGAAAAGAGCCGAA+TGG - Chr5:52879565-52879584 MsG0580027083.01.T01:intron 45.0%
TACCTAGTACGCAATGCAGA+TGG - Chr5:52879825-52879844 MsG0580027083.01.T01:intron 45.0%
TCATTCTGGTCGGTGAGATT+TGG - Chr5:52881097-52881116 MsG0580027083.01.T01:intron 45.0%
TCCCTGGATTGAGTAGAGAA+AGG - Chr5:52878286-52878305 MsG0580027083.01.T01:intron 45.0%
TCCGTACCATACAACATTGC+CGG - Chr5:52879304-52879323 MsG0580027083.01.T01:intron 45.0%
TCGGTAAAAGATACTCCCTC+TGG - Chr5:52878569-52878588 MsG0580027083.01.T01:intron 45.0%
TGACGAGTTCAACAGCTAAC+AGG + Chr5:52880752-52880771 None:intergenic 45.0%
TGAGATCCAAGAACTGTGGT+TGG - Chr5:52879737-52879756 MsG0580027083.01.T01:intron 45.0%
TGGAAGAGGAAAGTAGCACT+GGG + Chr5:52880114-52880133 None:intergenic 45.0%
TGTGAGAGGGTTCATGTTTC+TGG + Chr5:52877994-52878013 None:intergenic 45.0%
TGTGTCAAGTTCTTGGTGGA+GGG - Chr5:52879609-52879628 MsG0580027083.01.T01:CDS 45.0%
TGTTGTATGGTACGGAGTGT+TGG + Chr5:52879300-52879319 None:intergenic 45.0%
TTCAAGCTGGGTGGTTGAAA+TGG + Chr5:52879408-52879427 None:intergenic 45.0%
TTGAAATGGAGAAGAGCCTC+AGG + Chr5:52879394-52879413 None:intergenic 45.0%
TTGGAAGAGGAAAGTAGCAC+TGG + Chr5:52880115-52880134 None:intergenic 45.0%
! AAACGAGAGGAGCGTAGTTA+CGG + Chr5:52877955-52877974 None:intergenic 45.0%
! ATTCGGCTCTTTTCAGCCTT+AGG + Chr5:52879566-52879585 None:intergenic 45.0%
! CAACAACGTCACTTTGGAAG+AGG + Chr5:52880128-52880147 None:intergenic 45.0%
! GTTGTATGGTACGGAGTGTT+GGG + Chr5:52879299-52879318 None:intergenic 45.0%
! TAACTACGCTCCTCTCGTTT+TGG - Chr5:52877955-52877974 MsG0580027083.01.T01:three_prime_UTR 45.0%
!! TCTGGTTTGGATCCAGAACT+TGG - Chr5:52881151-52881170 MsG0580027083.01.T01:CDS 45.0%
ACCGACCAGAATGAGCAATG+TGG + Chr5:52881091-52881110 None:intergenic 50.0%
ACGCCTTATCCCACTAAGTG+GGG - Chr5:52878909-52878928 MsG0580027083.01.T01:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG + Chr5:52879232-52879251 None:intergenic 50.0%
ATCGATAGCAGAAGAGGAGG+AGG + Chr5:52878047-52878066 None:intergenic 50.0%
CAACGCCTTATCCCACTAAG+TGG - Chr5:52878907-52878926 MsG0580027083.01.T01:intron 50.0%
CGATGTTTCGCGGTTGAAGA+AGG + Chr5:52880260-52880279 None:intergenic 50.0%
CTCATTGCTTCTGATGTCCC+TGG - Chr5:52878270-52878289 MsG0580027083.01.T01:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - Chr5:52878913-52878932 MsG0580027083.01.T01:intron 50.0%
GACAGAGCTACGATGTTTCG+CGG + Chr5:52880270-52880289 None:intergenic 50.0%
GCCAACAATTAGACCTGGTC+TGG - Chr5:52881133-52881152 MsG0580027083.01.T01:intron 50.0%
GCCACATTGCTCATTCTGGT+CGG - Chr5:52881087-52881106 MsG0580027083.01.T01:intron 50.0%
GCGCAATTGAGTGAGTGAGA+GGG + Chr5:52878444-52878463 None:intergenic 50.0%
GCGGAGAGTCAGGTTAAAAG+AGG + Chr5:52879055-52879074 None:intergenic 50.0%
GTCAGACCGGCAATGTTGTA+TGG + Chr5:52879313-52879332 None:intergenic 50.0%
TATGGAGATTGGCGACCTTC+TGG - Chr5:52880516-52880535 MsG0580027083.01.T01:intron 50.0%
TCCAAAGTGACGTTGTTGGC+TGG - Chr5:52880130-52880149 MsG0580027083.01.T01:intron 50.0%
TCTTCTTACCACGGCGTCTA+CGG - Chr5:52879087-52879106 MsG0580027083.01.T01:intron 50.0%
TGAAAAGAGCCGAATGGAGC+AGG - Chr5:52879571-52879590 MsG0580027083.01.T01:intron 50.0%
TGGGAACGAACCAAAACGAG+AGG + Chr5:52877968-52877987 None:intergenic 50.0%
TTCTGGATCCAAACCAGACC+AGG + Chr5:52881149-52881168 None:intergenic 50.0%
TTGTACCTTGTGGCAGAGCA+TGG - Chr5:52880835-52880854 MsG0580027083.01.T01:CDS 50.0%
! AAGTAAGCGTAGCGGAGATG+AGG + Chr5:52879252-52879271 None:intergenic 50.0%
! ACCATCTGATGGTGTGCCTA+AGG - Chr5:52879547-52879566 MsG0580027083.01.T01:intron 50.0%
! AGATCAAATGGCGGAGAGTC+AGG + Chr5:52879065-52879084 None:intergenic 50.0%
! CTTTTCCATGCTCTGCCACA+AGG + Chr5:52880843-52880862 None:intergenic 50.0%
! GAGAGCATACCTTGAGAGTG+TGG + Chr5:52878084-52878103 None:intergenic 50.0%
!! AGGGTTCATGTTTCTGGGTC+TGG + Chr5:52877988-52878007 None:intergenic 50.0%
!! GGGTTCATGTTTCTGGGTCT+GGG + Chr5:52877987-52878006 None:intergenic 50.0%
!! TGAAAGGTGTCCTGTCCTCA+AGG - Chr5:52880070-52880089 MsG0580027083.01.T01:intron 50.0%
!! TGGTGCCACATTGCTCATTC+TGG - Chr5:52881083-52881102 MsG0580027083.01.T01:intron 50.0%
ACTAAGTGGGGTCGGCTACA+TGG - Chr5:52878921-52878940 MsG0580027083.01.T01:intron 55.0%
ATGTAGCCGACCCCACTTAG+TGG + Chr5:52878922-52878941 None:intergenic 55.0%
CACTCTCTGCCACACTCTCA+AGG - Chr5:52878072-52878091 MsG0580027083.01.T01:three_prime_UTR 55.0%
CGACCCCACTTAGTGGGATA+AGG + Chr5:52878915-52878934 None:intergenic 55.0%
CGCGCAATTGAGTGAGTGAG+AGG + Chr5:52878445-52878464 None:intergenic 55.0%
CGGAGATGAGGATGTTGCGT+TGG + Chr5:52879240-52879259 None:intergenic 55.0%
CTATCGGACAGCAGTCAGAC+CGG + Chr5:52879326-52879345 None:intergenic 55.0%
CTTCTTACCACGGCGTCTAC+GGG - Chr5:52879088-52879107 MsG0580027083.01.T01:intron 55.0%
GAGGACAGGACACCTTTCAG+AGG + Chr5:52880069-52880088 None:intergenic 55.0%
GATAGCAGAAGAGGAGGAGG+AGG + Chr5:52878044-52878063 None:intergenic 55.0%
GCCAGCCAACAACGTCACTT+TGG + Chr5:52880134-52880153 None:intergenic 55.0%
GCCTTAGGCACACCATCAGA+TGG + Chr5:52879551-52879570 None:intergenic 55.0%
GTTCTTGGTGGAGGGATGAG+AGG - Chr5:52879617-52879636 MsG0580027083.01.T01:CDS 55.0%
TGTAGCCGACCCCACTTAGT+GGG + Chr5:52878921-52878940 None:intergenic 55.0%
! CGCCGATGATGATGATGCAG+TGG + Chr5:52877896-52877915 None:intergenic 55.0%
AGAAGAGGAGGAGGAGGAGG+AGG + Chr5:52878038-52878057 None:intergenic 60.0%
AGCAGAAGAGGAGGAGGAGG+AGG + Chr5:52878041-52878060 None:intergenic 60.0%
! GAGGAGGAGGAGGTGTGAGA+GGG + Chr5:52878007-52878026 None:intergenic 60.0%
AGAGGAGGAGGAGGAGGAGG+AGG + Chr5:52878035-52878054 None:intergenic 65.0%
! GGAGGAGGAGGAGGTGTGAG+AGG + Chr5:52878008-52878027 None:intergenic 65.0%
GAGAAGGCCCGTAGACGCCG+TGG + Chr5:52879098-52879117 None:intergenic 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG + Chr5:52878014-52878033 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 52877818 52881409 52877818 ID=MsG0580027083.01;Name=MsG0580027083.01
Chr5 mRNA 52877818 52881409 52877818 ID=MsG0580027083.01.T01;Parent=MsG0580027083.01;Name=MsG0580027083.01.T01;_AED=0.41;_eAED=0.42;_QI=33|0.83|0.71|1|1|1|7|342|285
Chr5 exon 52877818 52878245 52877818 ID=MsG0580027083.01.T01:exon:29274;Parent=MsG0580027083.01.T01
Chr5 exon 52878335 52878376 52878335 ID=MsG0580027083.01.T01:exon:29273;Parent=MsG0580027083.01.T01
Chr5 exon 52878591 52878627 52878591 ID=MsG0580027083.01.T01:exon:29272;Parent=MsG0580027083.01.T01
Chr5 exon 52879415 52879494 52879415 ID=MsG0580027083.01.T01:exon:29271;Parent=MsG0580027083.01.T01
Chr5 exon 52879589 52879714 52879589 ID=MsG0580027083.01.T01:exon:29270;Parent=MsG0580027083.01.T01
Chr5 exon 52880801 52881044 52880801 ID=MsG0580027083.01.T01:exon:29269;Parent=MsG0580027083.01.T01
Chr5 exon 52881134 52881409 52881134 ID=MsG0580027083.01.T01:exon:29268;Parent=MsG0580027083.01.T01
Chr5 five_prime_UTR 52881377 52881409 52881377 ID=MsG0580027083.01.T01:five_prime_utr;Parent=MsG0580027083.01.T01
Chr5 CDS 52881134 52881376 52881134 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 CDS 52880801 52881044 52880801 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 CDS 52879589 52879714 52879589 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 CDS 52879415 52879494 52879415 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 CDS 52878591 52878627 52878591 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 CDS 52878335 52878376 52878335 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 CDS 52878160 52878245 52878160 ID=MsG0580027083.01.T01:cds;Parent=MsG0580027083.01.T01
Chr5 three_prime_UTR 52877818 52878159 52877818 ID=MsG0580027083.01.T01:three_prime_utr;Parent=MsG0580027083.01.T01
Gene Sequence

>MsG0580027083.01.T01

ATGGCGATTAATAATTACGCAAGCAGTCGGCATTGTATGTCACCACTGCATCATCATCATCGGCGTTCGATTCATGTTGTAACAAAACCCTTTGTTGTTCTCCGTAACTACGCTCCTCTCGTTTTGGTTCGTTCCCAGACCCAGAAACATGAACCCTCTCACACCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTCTGCTATCGATTTTCTCACTCTCTGCCACACTCTCAAGACCACAAAGAGGAAAGGATGGGTTAATCATGGGATTAAAGGGGCTGAATCCATAGCTGACCATATGTACCGTATGGCTTTGATGTCACTCATTGCTTCTGATGTCCCTGGATTGAGTAGAGAAAGGTTCACTGCTTTCACTTTCACTTTTTCTTCTTTCACATTTTCGTCTTGTCTCACTCCTTTGCTTTCTTCTGTGCTGTGCAGATGTATTAAAATTGCTCTCGTCCATGATATTGCAGAAGTGTTATACTCAATAGCTATTGTAGGAGATATAACACCATCTGATGGTGTGCCTAAGGCTGAAAAGAGCCGAATGGAGCAGGAAGCTTTAAACAAAATGTGTCAAGTTCTTGGTGGAGGGATGAGAGCTGATGAGATCCAAGAACTGTGGTTGGAGTATGAAAACAATTCTTCTTTAGAGGCTAATGTTGTTAAAGATTTTGACAAGGTTGAAATGATTCTACAAGCACTGGAATATGAAATGGAGCATGGAAAAGTGCTAGATGAATTTTTCATTTCAACTGCAGGGAAGTTTCAAACTGAAATAGGTAAAAGTTGGGCAGCCGAAATCATTTCAAGAAGAAAATCTTTATCAGCAAAAAAGTTAAGTTGA
Protein sequence

>MsG0580027083.01.T01

MAINNYASSRHCMSPLHHHHRRSIHVVTKPFVVLRNYAPLVLVRSQTQKHEPSHTSSSSSSSSSSSSSSAIDFLTLCHTLKTTKRKGWVNHGIKGAESIADHMYRMALMSLIASDVPGLSRERFTAFTFTFSSFTFSSCLTPLLSSVLCRCIKIALVHDIAEVLYSIAIVGDITPSDGVPKAEKSRMEQEALNKMCQVLGGGMRADEIQELWLEYENNSSLEANVVKDFDKVEMILQALEYEMEHGKVLDEFFISTAGKFQTEIGKSWAAEIISRRKSLSAKKLS*