Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027138.01.T01 | XP_003601257.1 | 80.645 | 62 | 12 | 0 | 5 | 66 | 103 | 164 | 4.04E-25 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027138.01.T01 | Q8LR34 | 61.29 | 62 | 24 | 0 | 5 | 66 | 110 | 171 | 1.73E-18 | 77 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027138.01.T01 | B7FHC6 | 80.645 | 62 | 12 | 0 | 5 | 66 | 103 | 164 | 1.93e-25 | 101 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580027138.01 | MsG0580027139.01 | 0.839394 | 1.626730e-57 | 2.243561e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027138.01.T01 | MTR_3g077720 | 80.645 | 62 | 12 | 0 | 5 | 66 | 103 | 164 | 4.90e-29 | 101 |
| MsG0580027138.01.T01 | MTR_3g077730 | 80.357 | 56 | 11 | 0 | 7 | 62 | 80 | 135 | 8.57e-26 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027138.01.T01 | AT4G22220 | 64.815 | 54 | 19 | 0 | 5 | 58 | 105 | 158 | 3.23e-19 | 76.3 |
| MsG0580027138.01.T01 | AT3G01020 | 54.839 | 62 | 28 | 0 | 5 | 66 | 102 | 163 | 7.81e-19 | 75.1 |
| MsG0580027138.01.T01 | AT4G04080 | 60.000 | 55 | 22 | 0 | 5 | 59 | 103 | 157 | 3.84e-18 | 73.6 |
Find 17 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCTGCAGTGAAGCTTAAC+TGG | 0.354352 | 5:-54265058 | None:intergenic |
| TGCCTCGGCTACTGCGGCCA+CGG | 0.446467 | 5:+54265132 | MsG0580027138.01.T01:CDS |
| GACGTGTAGCTAAGTAGCTT+CGG | 0.483936 | 5:-54264719 | None:intergenic |
| ATGATCGGCAACCAAGGAAA+AGG | 0.491231 | 5:+54264693 | MsG0580027138.01.T01:CDS |
| TTATATTTATTCTTAATGAT+CGG | 0.503747 | 5:+54264678 | None:intergenic |
| GCCTCGGCTACTGCGGCCAC+GGG | 0.517239 | 5:+54265133 | MsG0580027138.01.T01:CDS |
| TGCAGTGAAGCTTAACTGGC+GGG | 0.542392 | 5:-54265054 | None:intergenic |
| TCCCGTGGCCGCAGTAGCCG+AGG | 0.550070 | 5:-54265134 | None:intergenic |
| TTCTTAATGATCGGCAACCA+AGG | 0.566302 | 5:+54264687 | None:intergenic |
| TTATGAAGCTAAGCGTGCCT+CGG | 0.575006 | 5:+54265117 | MsG0580027138.01.T01:CDS |
| GCTACTTAGCTACACGTCGA+AGG | 0.596737 | 5:+54264724 | MsG0580027138.01.T01:CDS |
| TAAGCGTGCCTCGGCTACTG+CGG | 0.598393 | 5:+54265126 | MsG0580027138.01.T01:CDS |
| TCACTGCAGCATGCTTGCTG+AGG | 0.608636 | 5:+54265069 | MsG0580027138.01.T01:CDS |
| AGTGGCAGATTTGTCTCCCG+TGG | 0.628353 | 5:-54265149 | None:intergenic |
| CTGCAGTGAAGCTTAACTGG+CGG | 0.631259 | 5:-54265055 | None:intergenic |
| CAATGGGAGAAATCATGCAG+TGG | 0.672265 | 5:-54265167 | None:intergenic |
| TGAGGATGCTATAAAAGCAG+CGG | 0.695297 | 5:+54265087 | MsG0580027138.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAGATACATAATTTTTAG+TGG | + | Chr5:54264786-54264805 | MsG0580027138.01.T01:intron | 15.0% |
| !!! | TCCCAAATTTTAATAAATTT+GGG | - | Chr5:54264767-54264786 | None:intergenic | 15.0% |
| !!! | TTCCCAAATTTTAATAAATT+TGG | - | Chr5:54264768-54264787 | None:intergenic | 15.0% |
| !! | GCCCAAATTTATTAAAATTT+GGG | + | Chr5:54264763-54264782 | MsG0580027138.01.T01:intron | 20.0% |
| !! | TGCCCAAATTTATTAAAATT+TGG | + | Chr5:54264762-54264781 | MsG0580027138.01.T01:intron | 20.0% |
| !!! | CTACTAAATAGTTTTATCTT+TGG | + | Chr5:54264934-54264953 | MsG0580027138.01.T01:intron | 20.0% |
| ! | AACAAGAAAAAGAAACCAAA+AGG | - | Chr5:54264857-54264876 | None:intergenic | 25.0% |
| !!! | TGGTTTCTTTTTCTTGTTTT+TGG | + | Chr5:54264859-54264878 | MsG0580027138.01.T01:intron | 25.0% |
| !!! | TTCTTTTTCTTGTTTTTGGA+AGG | + | Chr5:54264863-54264882 | MsG0580027138.01.T01:intron | 25.0% |
| TAAGATTAAATCCTGCAGAT+CGG | - | Chr5:54264914-54264933 | None:intergenic | 30.0% | |
| !!! | TTTTTCTTGTTTTTGGAAGG+AGG | + | Chr5:54264866-54264885 | MsG0580027138.01.T01:intron | 30.0% |
| AACATAGCAGCAAAAGATGA+TGG | - | Chr5:54264829-54264848 | None:intergenic | 35.0% | |
| ! | CATCTTTTGCTGCTATGTTT+TGG | + | Chr5:54264830-54264849 | MsG0580027138.01.T01:intron | 35.0% |
| !! | TTTGGTAATGCTTCCATGAT+TGG | + | Chr5:54264952-54264971 | MsG0580027138.01.T01:intron | 35.0% |
| CAGACCAAAACTACCAATCA+TGG | - | Chr5:54264968-54264987 | None:intergenic | 40.0% | |
| TGAGGATGCTATAAAAGCAG+CGG | + | Chr5:54265087-54265106 | MsG0580027138.01.T01:CDS | 40.0% | |
| !! | CTGCTATGTTTTGGACCTTT+TGG | + | Chr5:54264839-54264858 | MsG0580027138.01.T01:intron | 40.0% |
| !! | GCTTCCATGATTGGTAGTTT+TGG | + | Chr5:54264961-54264980 | MsG0580027138.01.T01:intron | 40.0% |
| ATGATCGGCAACCAAGGAAA+AGG | + | Chr5:54264693-54264712 | MsG0580027138.01.T01:CDS | 45.0% | |
| ATGCTGCAGTGAAGCTTAAC+TGG | - | Chr5:54265061-54265080 | None:intergenic | 45.0% | |
| GACGTGTAGCTAAGTAGCTT+CGG | - | Chr5:54264722-54264741 | None:intergenic | 45.0% | |
| GCGCGATTCAGAAACCAAAA+GGG | - | Chr5:54264892-54264911 | None:intergenic | 45.0% | |
| TTATGAAGCTAAGCGTGCCT+CGG | + | Chr5:54265117-54265136 | MsG0580027138.01.T01:CDS | 45.0% | |
| ! | AGCTTCGGATTCCTTTTCCT+TGG | - | Chr5:54264707-54264726 | None:intergenic | 45.0% |
| !! | TTTTTGGAAGGAGGCCCTTT+TGG | + | Chr5:54264875-54264894 | MsG0580027138.01.T01:intron | 45.0% |
| CTGCAGTGAAGCTTAACTGG+CGG | - | Chr5:54265058-54265077 | None:intergenic | 50.0% | |
| GCTACTTAGCTACACGTCGA+AGG | + | Chr5:54264724-54264743 | MsG0580027138.01.T01:CDS | 50.0% | |
| GGCGCGATTCAGAAACCAAA+AGG | - | Chr5:54264893-54264912 | None:intergenic | 50.0% | |
| TGCAGTGAAGCTTAACTGGC+GGG | - | Chr5:54265057-54265076 | None:intergenic | 50.0% | |
| AGTGGCAGATTTGTCTCCCG+TGG | - | Chr5:54265152-54265171 | None:intergenic | 55.0% | |
| ! | TCACTGCAGCATGCTTGCTG+AGG | + | Chr5:54265069-54265088 | MsG0580027138.01.T01:CDS | 55.0% |
| TAAGCGTGCCTCGGCTACTG+CGG | + | Chr5:54265126-54265145 | MsG0580027138.01.T01:CDS | 60.0% | |
| ! | TCTGAATCGCGCCGATCTGC+AGG | + | Chr5:54264900-54264919 | MsG0580027138.01.T01:intron | 60.0% |
| TGCCTCGGCTACTGCGGCCA+CGG | + | Chr5:54265132-54265151 | MsG0580027138.01.T01:CDS | 70.0% | |
| GCCTCGGCTACTGCGGCCAC+GGG | + | Chr5:54265133-54265152 | MsG0580027138.01.T01:CDS | 75.0% | |
| TCCCGTGGCCGCAGTAGCCG+AGG | - | Chr5:54265137-54265156 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 54264693 | 54265177 | 54264693 | ID=MsG0580027138.01;Name=MsG0580027138.01 |
| Chr5 | mRNA | 54264693 | 54265177 | 54264693 | ID=MsG0580027138.01.T01;Parent=MsG0580027138.01;Name=MsG0580027138.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0|0.5|1|1|1|2|0|66 |
| Chr5 | exon | 54265030 | 54265177 | 54265030 | ID=MsG0580027138.01.T01:exon:40541;Parent=MsG0580027138.01.T01 |
| Chr5 | exon | 54264693 | 54264745 | 54264693 | ID=MsG0580027138.01.T01:exon:40540;Parent=MsG0580027138.01.T01 |
| Chr5 | CDS | 54264693 | 54264745 | 54264693 | ID=MsG0580027138.01.T01:cds;Parent=MsG0580027138.01.T01 |
| Chr5 | CDS | 54265030 | 54265177 | 54265030 | ID=MsG0580027138.01.T01:cds;Parent=MsG0580027138.01.T01 |
| Gene Sequence |
| Protein sequence |
