AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027139.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027139.01.T01 MTR_3g077720 82.927 164 27 1 1 163 1 164 1.05e-95 274
MsG0580027139.01.T01 MTR_3g077730 78.626 131 25 2 32 159 5 135 1.10e-67 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027139.01.T01 AT4G22220 64.968 157 52 3 1 155 3 158 9.81e-66 198
MsG0580027139.01.T01 AT4G04080 67.407 135 43 1 23 156 23 157 5.55e-64 194
MsG0580027139.01.T01 AT3G01020 65.000 140 48 1 25 163 24 163 9.01e-60 183

Find 64 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTAAGGTTGATGATAA+TGG 0.228001 5:+54269782 MsG0580027139.01.T01:CDS
TGTCATGAAGTTTCAAATTA+AGG 0.237273 5:+54269769 MsG0580027139.01.T01:CDS
AGTTGCCAATTCAAGCAATT+AGG 0.247880 5:+54269624 MsG0580027139.01.T01:CDS
ACGAGTTTCTTTGCGATTCT+CGG 0.306574 5:-54269584 None:intergenic
CCGAAGATCTTTCTTATTGA+AGG 0.314287 5:-54269711 None:intergenic
AGAACCCTAATTGCTTGAAT+TGG 0.316456 5:-54269629 None:intergenic
GATTCTTGCTTTAAGACCTT+TGG 0.331092 5:+54269815 MsG0580027139.01.T01:CDS
TGTTTGAATGGTTTGTTTGT+AGG 0.334004 5:+54270580 MsG0580027139.01.T01:three_prime_UTR
TGCATGATTTCTCCCATTGC+TGG 0.335905 5:+54270328 MsG0580027139.01.T01:exon
GGTAAAGGGAAAGCAAATTA+AGG 0.338517 5:+54270026 MsG0580027139.01.T01:CDS
TACAAATACTTGTGTCGGTT+TGG 0.345859 5:+54270491 MsG0580027139.01.T01:three_prime_UTR
ATGCTGCAGTGAAGCTTAAC+TGG 0.354352 5:-54270215 None:intergenic
GTTGCCAATTCAAGCAATTA+GGG 0.356677 5:+54269625 MsG0580027139.01.T01:CDS
CGCAAAGAAACTCGTGCAAA+CGG 0.368310 5:+54269592 MsG0580027139.01.T01:CDS
TTCTCCCATTGCTGGAATGC+TGG 0.368918 5:+54270336 MsG0580027139.01.T01:three_prime_UTR
GATGAAGTTTCTTGTTTGAA+TGG 0.380469 5:+54270568 MsG0580027139.01.T01:three_prime_UTR
GTCTTTATGACAATACAATA+TGG 0.381045 5:-54270524 None:intergenic
CTTCAATAAGAAAGATCTTC+GGG 0.392132 5:+54269712 MsG0580027139.01.T01:CDS
GCAAAGAAACTCGTGCAAAC+GGG 0.401810 5:+54269593 MsG0580027139.01.T01:CDS
ATTGAAGGATCCAACGTTCC+GGG 0.412834 5:-54269696 None:intergenic
TATTGAAGGATCCAACGTTC+CGG 0.415466 5:-54269697 None:intergenic
GCAGCTACTGAATGGGTAAA+GGG 0.419402 5:+54270012 MsG0580027139.01.T01:intron
CTTATAAATACTTGTATCGT+TGG 0.431923 5:+54270546 MsG0580027139.01.T01:three_prime_UTR
TGCCTCGGCTACTGCGGCCA+CGG 0.446467 5:+54270289 MsG0580027139.01.T01:CDS
ACTTGTGTCGGTTTGGAGCT+AGG 0.449526 5:+54270498 MsG0580027139.01.T01:three_prime_UTR
CCTTCAATAAGAAAGATCTT+CGG 0.452805 5:+54269711 MsG0580027139.01.T01:CDS
TGCTTTAAGACCTTTGGATG+TGG 0.461939 5:+54269821 MsG0580027139.01.T01:CDS
TGCAGCTACTGAATGGGTAA+AGG 0.478916 5:+54270011 MsG0580027139.01.T01:intron
CTTCGGGAGGTGGGATCTGT+TGG 0.484742 5:+54269728 MsG0580027139.01.T01:CDS
TAAGAAAGATCTTCGGGAGG+TGG 0.491830 5:+54269718 MsG0580027139.01.T01:CDS
GATGTTATAACAATACAATA+TGG 0.496765 5:-54270467 None:intergenic
TTCAACGATTCTACAACAAG+AGG 0.500606 5:+54269651 MsG0580027139.01.T01:CDS
CATTCCAGCATTCCAGCAAT+GGG 0.505665 5:-54270340 None:intergenic
GCCTCGGCTACTGCGGCCAC+GGG 0.517239 5:+54270290 MsG0580027139.01.T01:CDS
TCATTCCAGCATTCCAGCAA+TGG 0.517945 5:-54270341 None:intergenic
CATCTTACAAATACTTGTGT+CGG 0.525946 5:+54270486 MsG0580027139.01.T01:three_prime_UTR
TGCAGTGAAGCTTAACTGGC+GGG 0.542392 5:-54270211 None:intergenic
TCCCGTGGCCGCAGTAGCCG+AGG 0.550070 5:-54270291 None:intergenic
ATGATCCTTACAGCTAGCAT+AGG 0.552184 5:-54270646 None:intergenic
TACAACAATCCCCGGAACGT+TGG 0.563672 5:+54269686 MsG0580027139.01.T01:CDS
AATCGACTCATTAGCACCAT+TGG 0.564226 5:-54270392 None:intergenic
AAGAAAGATCTTCGGGAGGT+GGG 0.571174 5:+54269719 MsG0580027139.01.T01:CDS
GCTATTGCTTCATCGTCCGT+TGG 0.572466 5:+54269848 MsG0580027139.01.T01:CDS
TTATGAAGCTAAGCGTGCCT+CGG 0.575006 5:+54270274 MsG0580027139.01.T01:CDS
CTTCATGACATCACCACAAG+CGG 0.586806 5:-54269756 None:intergenic
TGATCCTTACAGCTAGCATA+GGG 0.594333 5:-54270645 None:intergenic
TTGAAGGATCCAACGTTCCG+GGG 0.597622 5:-54269695 None:intergenic
TAAGCGTGCCTCGGCTACTG+CGG 0.598393 5:+54270283 MsG0580027139.01.T01:CDS
CAAAGAAACTCGTGCAAACG+GGG 0.599049 5:+54269594 MsG0580027139.01.T01:CDS
TTCATGACATCACCACAAGC+GGG 0.599308 5:-54269755 None:intergenic
TCATTAGTATGATTCTCCAA+TGG 0.600860 5:+54270376 MsG0580027139.01.T01:three_prime_UTR
TGGATCACTACAACAATCCC+CGG 0.601326 5:+54269678 MsG0580027139.01.T01:CDS
TCACTGCAGCATGCTTGCTG+AGG 0.608636 5:+54270226 MsG0580027139.01.T01:CDS
TCTGTTGGTGCACCCGCTTG+TGG 0.618008 5:+54269743 MsG0580027139.01.T01:CDS
ATTCTACAACAAGAGGGTGG+TGG 0.622053 5:+54269658 MsG0580027139.01.T01:CDS
AGTGGCAGATTTGTCTCCCG+TGG 0.628353 5:-54270306 None:intergenic
CTGCAGTGAAGCTTAACTGG+CGG 0.631259 5:-54270212 None:intergenic
AATAGCGGAACCACATCCAA+AGG 0.639771 5:-54269831 None:intergenic
TCAACGATTCTACAACAAGA+GGG 0.652759 5:+54269652 MsG0580027139.01.T01:CDS
CAATGGGAGAAATCATGCAG+TGG 0.672265 5:-54270324 None:intergenic
AACGGACGATGAAGCAATAG+CGG 0.675366 5:-54269846 None:intergenic
TGAGGATGCTATAAAAGCAG+CGG 0.695297 5:+54270244 MsG0580027139.01.T01:CDS
CAATAAGAAAGATCTTCGGG+AGG 0.711849 5:+54269715 MsG0580027139.01.T01:CDS
ACGATTCTACAACAAGAGGG+TGG 0.727989 5:+54269655 MsG0580027139.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAAGGATTTTTTTTTT+AGG + Chr5:54269905-54269924 MsG0580027139.01.T01:intron 10.0%
!!! AATTAAGGATTTTTTTTTTA+GGG + Chr5:54269906-54269925 MsG0580027139.01.T01:intron 10.0%
!! TAACAAAGTAAAAAATTCTT+AGG - Chr5:54270086-54270105 None:intergenic 15.0%
!!! TATTCAGAATTTTCTTAATT+GGG + Chr5:54270435-54270454 MsG0580027139.01.T01:three_prime_UTR 15.0%
!!! TTATTCAGAATTTTCTTAAT+TGG + Chr5:54270434-54270453 MsG0580027139.01.T01:three_prime_UTR 15.0%
!! GATGTTATAACAATACAATA+TGG - Chr5:54270470-54270489 None:intergenic 20.0%
!!! ATTCAGAATTTTCTTAATTG+GGG + Chr5:54270436-54270455 MsG0580027139.01.T01:three_prime_UTR 20.0%
! CTTATAAATACTTGTATCGT+TGG + Chr5:54270546-54270565 MsG0580027139.01.T01:three_prime_UTR 25.0%
! GAATTTGATTATTACGACTA+TGG + Chr5:54269960-54269979 MsG0580027139.01.T01:intron 25.0%
! GTCTTTATGACAATACAATA+TGG - Chr5:54270527-54270546 None:intergenic 25.0%
! TGTCATGAAGTTTCAAATTA+AGG + Chr5:54269769-54269788 MsG0580027139.01.T01:CDS 25.0%
!!! CAAATTAAGGTTGATGATAA+TGG + Chr5:54269782-54269801 MsG0580027139.01.T01:CDS 25.0%
!!! CTACTAAACAGTTTTATCTT+TGG + Chr5:54270112-54270131 MsG0580027139.01.T01:intron 25.0%
!!! TAGGTTGTTTTTTCATGTAT+TGG + Chr5:54269871-54269890 MsG0580027139.01.T01:intron 25.0%
!!! TGGTTTGTTATAGGAAATTA+AGG + Chr5:54269891-54269910 MsG0580027139.01.T01:intron 25.0%
!!! TTCATGTATTGGTTTGTTAT+AGG + Chr5:54269882-54269901 MsG0580027139.01.T01:intron 25.0%
ATGAAAAAACAACCTACCAA+CGG - Chr5:54269867-54269886 None:intergenic 30.0%
ATTAAGAACACGTAAGTAAC+TGG + Chr5:54270060-54270079 MsG0580027139.01.T01:intron 30.0%
CATCTTACAAATACTTGTGT+CGG + Chr5:54270486-54270505 MsG0580027139.01.T01:three_prime_UTR 30.0%
CCTTCAATAAGAAAGATCTT+CGG + Chr5:54269711-54269730 MsG0580027139.01.T01:CDS 30.0%
CTTCAATAAGAAAGATCTTC+GGG + Chr5:54269712-54269731 MsG0580027139.01.T01:CDS 30.0%
TCATTAGTATGATTCTCCAA+TGG + Chr5:54270376-54270395 MsG0580027139.01.T01:three_prime_UTR 30.0%
! GATGAAGTTTCTTGTTTGAA+TGG + Chr5:54270568-54270587 MsG0580027139.01.T01:three_prime_UTR 30.0%
!! TGTTTGAATGGTTTGTTTGT+AGG + Chr5:54270580-54270599 MsG0580027139.01.T01:three_prime_UTR 30.0%
ACTTCCAAAACTACCAATCA+TGG - Chr5:54270146-54270165 None:intergenic 35.0%
AGAACCCTAATTGCTTGAAT+TGG - Chr5:54269632-54269651 None:intergenic 35.0%
AGTTGCCAATTCAAGCAATT+AGG + Chr5:54269624-54269643 MsG0580027139.01.T01:CDS 35.0%
CCGAAGATCTTTCTTATTGA+AGG - Chr5:54269714-54269733 None:intergenic 35.0%
GGTAAAGGGAAAGCAAATTA+AGG + Chr5:54270026-54270045 MsG0580027139.01.T01:CDS 35.0%
GTTGCCAATTCAAGCAATTA+GGG + Chr5:54269625-54269644 MsG0580027139.01.T01:CDS 35.0%
TCAACGATTCTACAACAAGA+GGG + Chr5:54269652-54269671 MsG0580027139.01.T01:CDS 35.0%
TTCAACGATTCTACAACAAG+AGG + Chr5:54269651-54269670 MsG0580027139.01.T01:CDS 35.0%
! GAATTTTCTTAATTGGGGCT+AGG + Chr5:54270441-54270460 MsG0580027139.01.T01:three_prime_UTR 35.0%
!! GATTCTTGCTTTAAGACCTT+TGG + Chr5:54269815-54269834 MsG0580027139.01.T01:CDS 35.0%
!! TACAAATACTTGTGTCGGTT+TGG + Chr5:54270491-54270510 MsG0580027139.01.T01:three_prime_UTR 35.0%
!! TTTGGTAATGCTTCCATGAT+TGG + Chr5:54270130-54270149 MsG0580027139.01.T01:intron 35.0%
ACGAGTTTCTTTGCGATTCT+CGG - Chr5:54269587-54269606 None:intergenic 40.0%
ATGATCCTTACAGCTAGCAT+AGG - Chr5:54270649-54270668 None:intergenic 40.0%
CAATAAGAAAGATCTTCGGG+AGG + Chr5:54269715-54269734 MsG0580027139.01.T01:CDS 40.0%
TATTGAAGGATCCAACGTTC+CGG - Chr5:54269700-54269719 None:intergenic 40.0%
TGAGGATGCTATAAAAGCAG+CGG + Chr5:54270244-54270263 MsG0580027139.01.T01:CDS 40.0%
TGATCCTTACAGCTAGCATA+GGG - Chr5:54270648-54270667 None:intergenic 40.0%
! AATCGACTCATTAGCACCAT+TGG - Chr5:54270395-54270414 None:intergenic 40.0%
! ATCGTTTTGCAGCTACTGAA+TGG + Chr5:54270004-54270023 MsG0580027139.01.T01:intron 40.0%
! TCGTTTTGCAGCTACTGAAT+GGG + Chr5:54270005-54270024 MsG0580027139.01.T01:intron 40.0%
! TTTTCCCTATGCTAGCTGTA+AGG + Chr5:54270641-54270660 MsG0580027139.01.T01:three_prime_UTR 40.0%
!! GCTTCCATGATTGGTAGTTT+TGG + Chr5:54270139-54270158 MsG0580027139.01.T01:intron 40.0%
!! TGCTTTAAGACCTTTGGATG+TGG + Chr5:54269821-54269840 MsG0580027139.01.T01:CDS 40.0%
AACGGACGATGAAGCAATAG+CGG - Chr5:54269849-54269868 None:intergenic 45.0%
AAGAAAGATCTTCGGGAGGT+GGG + Chr5:54269719-54269738 MsG0580027139.01.T01:CDS 45.0%
ACGATTCTACAACAAGAGGG+TGG + Chr5:54269655-54269674 MsG0580027139.01.T01:CDS 45.0%
ATGCTGCAGTGAAGCTTAAC+TGG - Chr5:54270218-54270237 None:intergenic 45.0%
ATTCTACAACAAGAGGGTGG+TGG + Chr5:54269658-54269677 MsG0580027139.01.T01:CDS 45.0%
ATTGAAGGATCCAACGTTCC+GGG - Chr5:54269699-54269718 None:intergenic 45.0%
CAAAGAAACTCGTGCAAACG+GGG + Chr5:54269594-54269613 MsG0580027139.01.T01:CDS 45.0%
CAATGGGAGAAATCATGCAG+TGG - Chr5:54270327-54270346 None:intergenic 45.0%
CATTCCAGCATTCCAGCAAT+GGG - Chr5:54270343-54270362 None:intergenic 45.0%
CGCAAAGAAACTCGTGCAAA+CGG + Chr5:54269592-54269611 MsG0580027139.01.T01:CDS 45.0%
CTTCATGACATCACCACAAG+CGG - Chr5:54269759-54269778 None:intergenic 45.0%
GCAAAGAAACTCGTGCAAAC+GGG + Chr5:54269593-54269612 MsG0580027139.01.T01:CDS 45.0%
GCAGCTACTGAATGGGTAAA+GGG + Chr5:54270012-54270031 MsG0580027139.01.T01:intron 45.0%
TAAGAAAGATCTTCGGGAGG+TGG + Chr5:54269718-54269737 MsG0580027139.01.T01:CDS 45.0%
TCATTCCAGCATTCCAGCAA+TGG - Chr5:54270344-54270363 None:intergenic 45.0%
TGCAGCTACTGAATGGGTAA+AGG + Chr5:54270011-54270030 MsG0580027139.01.T01:intron 45.0%
TGCATGATTTCTCCCATTGC+TGG + Chr5:54270328-54270347 MsG0580027139.01.T01:exon 45.0%
TGGATCACTACAACAATCCC+CGG + Chr5:54269678-54269697 MsG0580027139.01.T01:CDS 45.0%
TTATGAAGCTAAGCGTGCCT+CGG + Chr5:54270274-54270293 MsG0580027139.01.T01:CDS 45.0%
TTCATGACATCACCACAAGC+GGG - Chr5:54269758-54269777 None:intergenic 45.0%
! AATAGCGGAACCACATCCAA+AGG - Chr5:54269834-54269853 None:intergenic 45.0%
CTGCAGTGAAGCTTAACTGG+CGG - Chr5:54270215-54270234 None:intergenic 50.0%
GCTATTGCTTCATCGTCCGT+TGG + Chr5:54269848-54269867 MsG0580027139.01.T01:CDS 50.0%
TACAACAATCCCCGGAACGT+TGG + Chr5:54269686-54269705 MsG0580027139.01.T01:CDS 50.0%
TGCAGTGAAGCTTAACTGGC+GGG - Chr5:54270214-54270233 None:intergenic 50.0%
TTCTCCCATTGCTGGAATGC+TGG + Chr5:54270336-54270355 MsG0580027139.01.T01:three_prime_UTR 50.0%
TTGAAGGATCCAACGTTCCG+GGG - Chr5:54269698-54269717 None:intergenic 50.0%
TTGCTTCATCGTCCGTTGGT+AGG + Chr5:54269852-54269871 MsG0580027139.01.T01:intron 50.0%
!! ACTTGTGTCGGTTTGGAGCT+AGG + Chr5:54270498-54270517 MsG0580027139.01.T01:three_prime_UTR 50.0%
AGTGGCAGATTTGTCTCCCG+TGG - Chr5:54270309-54270328 None:intergenic 55.0%
! TCACTGCAGCATGCTTGCTG+AGG + Chr5:54270226-54270245 MsG0580027139.01.T01:CDS 55.0%
CTTCGGGAGGTGGGATCTGT+TGG + Chr5:54269728-54269747 MsG0580027139.01.T01:CDS 60.0%
TAAGCGTGCCTCGGCTACTG+CGG + Chr5:54270283-54270302 MsG0580027139.01.T01:CDS 60.0%
TCTGTTGGTGCACCCGCTTG+TGG + Chr5:54269743-54269762 MsG0580027139.01.T01:CDS 60.0%
TGCCTCGGCTACTGCGGCCA+CGG + Chr5:54270289-54270308 MsG0580027139.01.T01:CDS 70.0%
GCCTCGGCTACTGCGGCCAC+GGG + Chr5:54270290-54270309 MsG0580027139.01.T01:CDS 75.0%
TCCCGTGGCCGCAGTAGCCG+AGG - Chr5:54270294-54270313 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 54269581 54270692 54269581 ID=MsG0580027139.01;Name=MsG0580027139.01
Chr5 mRNA 54269581 54270692 54269581 ID=MsG0580027139.01.T01;Parent=MsG0580027139.01;Name=MsG0580027139.01.T01;_AED=0.45;_eAED=0.45;_QI=0|1|1|1|1|1|3|358|163
Chr5 exon 54270187 54270692 54270187 ID=MsG0580027139.01.T01:exon:40539;Parent=MsG0580027139.01.T01
Chr5 exon 54270016 54270070 54270016 ID=MsG0580027139.01.T01:exon:40538;Parent=MsG0580027139.01.T01
Chr5 exon 54269581 54269869 54269581 ID=MsG0580027139.01.T01:exon:40537;Parent=MsG0580027139.01.T01
Chr5 CDS 54269581 54269869 54269581 ID=MsG0580027139.01.T01:cds;Parent=MsG0580027139.01.T01
Chr5 CDS 54270016 54270070 54270016 ID=MsG0580027139.01.T01:cds;Parent=MsG0580027139.01.T01
Chr5 CDS 54270187 54270334 54270187 ID=MsG0580027139.01.T01:cds;Parent=MsG0580027139.01.T01
Chr5 three_prime_UTR 54270335 54270692 54270335 ID=MsG0580027139.01.T01:three_prime_utr;Parent=MsG0580027139.01.T01
Gene Sequence

>MsG0580027139.01.T01

ATGCCGAGAATCGCAAAGAAACTCGTGCAAACGGGGTCGTTTGAGTTGCCAATTCAAGCAATTAGGGTTCTTCAACGATTCTACAACAAGAGGGTGGTGGATCACTACAACAATCCCCGGAACGTTGGATCCTTCAATAAGAAAGATCTTCGGGAGGTGGGATCTGTTGGTGCACCCGCTTGTGGTGATGTCATGAAGTTTCAAATTAAGGTTGATGATAATGGAACAATCGTTGATTCTTGCTTTAAGACCTTTGGATGTGGTTCCGCTATTGCTTCATCGTCCGTTGCTACTGAATGGGTAAAGGGAAAGCAAATTAAGGAAGTTTTAAGCATTAAGAACACTGACATTGCAAAGCATCTTAAACTCCCGCCAGTTAAGCTTCACTGCAGCATGCTTGCTGAGGATGCTATAAAAGCAGCGGTTAAAGATTATGAAGCTAAGCGTGCCTCGGCTACTGCGGCCACGGGAGACAAATCTGCCACTGCATGA

Protein sequence

>MsG0580027139.01.T01

MPRIAKKLVQTGSFELPIQAIRVLQRFYNKRVVDHYNNPRNVGSFNKKDLREVGSVGAPACGDVMKFQIKVDDNGTIVDSCFKTFGCGSAIASSSVATEWVKGKQIKEVLSIKNTDIAKHLKLPPVKLHCSMLAEDAIKAAVKDYEAKRASATAATGDKSATA*