AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027241.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027241.01.T01 MTR_0002s0900 91.120 259 13 1 1 259 1 249 5.81e-74 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 116 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACTCTAAGCATTTATT+TGG 0.110578 5:+56859835 MsG0580027241.01.T01:three_prime_UTR
AAAATTTGTTTCTTATTTAT+TGG 0.170736 5:-56858818 None:intergenic
GCAAAATGTAAAATCATTTC+TGG 0.203021 5:+56859861 MsG0580027241.01.T01:three_prime_UTR
TCTTTGCTCGGGAACATTAA+TGG 0.234396 5:+56859943 MsG0580027241.01.T01:three_prime_UTR
ACCAGCACCTAGTCTTAATT+TGG 0.252717 5:+56859537 MsG0580027241.01.T01:CDS
GTGCAGGGGCTGGTGGTGTT+TGG 0.254459 5:-56859252 MsG0580027240.01.T01:CDS
GGAGTTGGCGCTTGCTTTAC+GGG 0.258542 5:-56859307 MsG0580027240.01.T01:CDS
GGCACCGGTGTTGCCTTTAC+TGG 0.259559 5:-56859274 MsG0580027240.01.T01:CDS
AACTTTGTTGGTGCAGTTGC+TGG 0.294409 5:-56858944 MsG0580027240.01.T01:CDS
AGGAGTTGGCGCTTGCTTTA+CGG 0.297656 5:-56859308 MsG0580027240.01.T01:CDS
CAACAATGGCTACAATGTTC+TGG 0.312570 5:+56858870 MsG0580027241.01.T01:exon
GGCGCTTGCTTTACGGGTGC+AGG 0.313651 5:-56859301 MsG0580027240.01.T01:CDS
GCCTTTACTGGTGCAGGGGC+TGG 0.315701 5:-56859262 MsG0580027240.01.T01:CDS
GTAGTGTCAGTTGGTGGTGC+AGG 0.317908 5:-56859490 MsG0580027240.01.T01:intron
TGCTTTACGGGTGCAGGTGC+TGG 0.319612 5:-56859295 MsG0580027240.01.T01:CDS
GTTGTTGCCGTCGGGGTTGT+AGG 0.332649 5:-56858971 MsG0580027240.01.T01:CDS
GATTTAGGTGCCACAGTAAC+AGG 0.340587 5:-56859052 MsG0580027240.01.T01:CDS
CTTGAAGCTGGTGCAACCTT+TGG 0.345396 5:-56859097 MsG0580027240.01.T01:intron
GGTTGAGGTGGTGAGACTTT+TGG 0.361599 5:-56859121 MsG0580027240.01.T01:intron
GCAGGCGTTGGTGCGGGTAT+TGG 0.363471 5:-56859349 MsG0580027240.01.T01:CDS
GTTGTAGGTGGCAACTTTGT+TGG 0.367631 5:-56858956 MsG0580027240.01.T01:CDS
ACTGGTGTTGGCGATATCTT+TGG 0.367896 5:-56859229 MsG0580027240.01.T01:CDS
GGTGTTGCCTTTACTGGTGC+AGG 0.376572 5:-56859268 MsG0580027240.01.T01:CDS
GGTACAGGTGCTTCTATTGC+AGG 0.381012 5:-56859367 MsG0580027240.01.T01:CDS
TTTGGAGCTGGTGATGTAAC+TGG 0.384051 5:-56859079 MsG0580027240.01.T01:intron
TTCACTCTAAGCATTTATTT+GGG 0.388223 5:+56859836 MsG0580027241.01.T01:three_prime_UTR
GTGTCTTCTTCTCTTGTGCT+TGG 0.394153 5:-56859404 MsG0580027240.01.T01:intron
GGAAATATATGGACAACAAT+TGG 0.401495 5:+56859744 MsG0580027241.01.T01:CDS
TTGGTGCAGTTGCTGGTGCT+TGG 0.402362 5:-56858937 MsG0580027240.01.T01:CDS
GGGGCTGGTGGTGTTTGGAC+TGG 0.403895 5:-56859247 MsG0580027240.01.T01:CDS
TGACCGGTGTTGTTGCCGTC+GGG 0.404756 5:-56858979 MsG0580027240.01.T01:CDS
CTAAGCCTCAGTTGCCTGCT+AGG 0.409371 5:+56858905 MsG0580027241.01.T01:CDS
TAACAAGGAACGTTAACTAA+TGG 0.419485 5:-56858696 None:intergenic
GGTATTGGTGGCGGAGTAGC+AGG 0.423733 5:-56859334 MsG0580027240.01.T01:CDS
GGTGGTGTTTGGACTGGTGT+TGG 0.441567 5:-56859241 MsG0580027240.01.T01:CDS
GGCAACGGTGGTGGCAATGT+TGG 0.443251 5:-56859169 MsG0580027240.01.T01:CDS
TCTATTGCAGGCGTTGGTGC+GGG 0.447779 5:-56859355 MsG0580027240.01.T01:CDS
TAGTGTCAGTTGGTGGTGCA+GGG 0.449666 5:-56859489 MsG0580027240.01.T01:intron
GGTGGTGGTGGCAACGGTGG+TGG 0.450498 5:-56859178 MsG0580027240.01.T01:CDS
AACAATGGCTACAATGTTCT+GGG 0.454166 5:+56858871 MsG0580027241.01.T01:exon
TGGTGCAGTTGCTGGTGCTT+GGG 0.457269 5:-56858936 MsG0580027240.01.T01:CDS
GTGTTGCCTTTACTGGTGCA+GGG 0.457541 5:-56859267 MsG0580027240.01.T01:CDS
TATTTGTCATGATCTTTGCT+CGG 0.463205 5:+56859931 MsG0580027241.01.T01:three_prime_UTR
TTGTGAATAACGGTCGGTGC+TGG 0.468332 5:-56859460 MsG0580027240.01.T01:intron
GGAGTAGCAGGTGCAGGAGT+TGG 0.469009 5:-56859322 MsG0580027240.01.T01:CDS
GCACCATCAAATCATCTTCA+AGG 0.474299 5:+56859720 MsG0580027241.01.T01:CDS
TGCTTGGAGGACGCAGGTGC+TGG 0.482794 5:-56859388 MsG0580027240.01.T01:intron
GGCGTTGGTGCGGGTATTGG+TGG 0.482974 5:-56859346 MsG0580027240.01.T01:CDS
TCTTCTTCTCTTGTGCTTGG+AGG 0.488609 5:-56859401 MsG0580027240.01.T01:intron
ATTTGTCATGATCTTTGCTC+GGG 0.489414 5:+56859932 MsG0580027241.01.T01:three_prime_UTR
GAGGACGCAGGTGCTGGTAC+AGG 0.492106 5:-56859382 MsG0580027240.01.T01:intron
TTTACTGGTGCAGGGGCTGG+TGG 0.492899 5:-56859259 MsG0580027240.01.T01:CDS
GGTGATGCAACAGGTGGTGG+TGG 0.494831 5:-56859190 MsG0580027240.01.T01:CDS
ACCAGCCCCTGCACCAGTAA+AGG 0.495470 5:+56859261 MsG0580027241.01.T01:CDS
ACGGGTGCAGGTGCTGGCAC+CGG 0.501284 5:-56859289 MsG0580027240.01.T01:CDS
GATGTAACTGGAGCAGATTT+AGG 0.502592 5:-56859067 MsG0580027240.01.T01:intron
TTCTATTGCAGGCGTTGGTG+CGG 0.508108 5:-56859356 MsG0580027240.01.T01:CDS
GGTGCTTCTATTGCAGGCGT+TGG 0.508992 5:-56859361 MsG0580027240.01.T01:CDS
TGCTGGTGCCGGTGCTGTGT+CGG 0.511565 5:-56859521 MsG0580027240.01.T01:CDS
GGTGGTGATGCAACAGGTGG+TGG 0.512309 5:-56859193 MsG0580027240.01.T01:CDS
GCAACAGGTGGTGGTGGCAA+CGG 0.513205 5:-56859184 MsG0580027240.01.T01:CDS
ATAATGTCACAATCAAATGA+AGG 0.515745 5:+56858731 MsG0580027241.01.T01:five_prime_UTR
AACGTTGGTGGTGATGCAAC+AGG 0.518979 5:-56859199 MsG0580027240.01.T01:CDS
TGGCTATATGATCAAGCAGA+TGG 0.530374 5:-56858798 None:intergenic
GTTGGTGCGGGTATTGGTGG+CGG 0.533550 5:-56859343 MsG0580027240.01.T01:CDS
ATAACGGTCGGTGCTGGAGC+TGG 0.534534 5:-56859454 MsG0580027240.01.T01:intron
CTCTTGTGCTTGGAGGACGC+AGG 0.534666 5:-56859394 MsG0580027240.01.T01:intron
GCTGGTGCAACCTTTGGAGC+TGG 0.545571 5:-56859091 MsG0580027240.01.T01:intron
ACAGGTGGTGGTGGCAACGG+TGG 0.545889 5:-56859181 MsG0580027240.01.T01:CDS
GGCAATGTTGGAGTTGAGAC+AGG 0.546727 5:-56859157 MsG0580027240.01.T01:intron
CACCATCAAATCATCTTCAA+GGG 0.548248 5:+56859721 MsG0580027241.01.T01:CDS
CTTGGGCGTTGAATCCTAGC+AGG 0.551054 5:-56858919 MsG0580027240.01.T01:CDS
TTTGGTGGTGGTAACGTTGG+TGG 0.551419 5:-56859211 MsG0580027240.01.T01:CDS
ATGACCGGTGTTGTTGCCGT+CGG 0.551478 5:-56858980 MsG0580027240.01.T01:CDS
GTGTCAGTTGGTGGTGCAGG+GGG 0.563770 5:-56859487 MsG0580027240.01.T01:intron
GACATGTAACTCATAACTAT+CGG 0.564959 5:-56860013 MsG0580027240.01.T01:intron
AGTTACATCACCAGCTCCAA+AGG 0.565349 5:+56859081 MsG0580027241.01.T01:CDS
ATCTTTGGTGGTGGTAACGT+TGG 0.566199 5:-56859214 MsG0580027240.01.T01:CDS
GGTGGCGGAGTAGCAGGTGC+AGG 0.568762 5:-56859328 MsG0580027240.01.T01:CDS
GGAGGAGATGCTGCAACGGT+CGG 0.571300 5:-56859013 MsG0580027240.01.T01:CDS
GAAGACACAGACACAAGCAC+AGG 0.572543 5:+56859419 MsG0580027241.01.T01:CDS
GGTGTTGGCGATATCTTTGG+TGG 0.578501 5:-56859226 MsG0580027240.01.T01:CDS
AAGCCAACCACCTGTTACTG+TGG 0.578992 5:+56859042 MsG0580027241.01.T01:CDS
AGTGTCAGTTGGTGGTGCAG+GGG 0.580128 5:-56859488 MsG0580027240.01.T01:intron
GAATCTGCTGTAGTGTCAGT+TGG 0.580132 5:-56859499 MsG0580027240.01.T01:CDS
GGAGATGCTGCAACGGTCGG+TGG 0.580831 5:-56859010 MsG0580027240.01.T01:CDS
GGCTATATGATCAAGCAGAT+GGG 0.584327 5:-56858797 None:intergenic
TTAACTAATGGAAAATGTGT+AGG 0.585170 5:-56858684 None:intergenic
GTCGGTGGTTGTGTCATGAC+CGG 0.585789 5:-56858995 MsG0580027240.01.T01:CDS
ACCAAATTAAGACTAGGTGC+TGG 0.590933 5:-56859538 MsG0580027240.01.T01:intron
TAATGTCACAATCAAATGAA+GGG 0.591483 5:+56858732 MsG0580027241.01.T01:five_prime_UTR
TCTGCTGTAGTGTCAGTTGG+TGG 0.593136 5:-56859496 MsG0580027240.01.T01:intron
TTAAGACTAGGTGCTGGTGC+CGG 0.593527 5:-56859532 MsG0580027240.01.T01:intron
GTGCCACAGTAACAGGTGGT+TGG 0.594519 5:-56859045 MsG0580027240.01.T01:CDS
GATGGGAGGAGATGCTGCAA+CGG 0.596294 5:-56859017 MsG0580027240.01.T01:CDS
GTTGGCGATATCTTTGGTGG+TGG 0.598175 5:-56859223 MsG0580027240.01.T01:CDS
ATGGGTAGAAACGTATGTAG+TGG 0.600710 5:-56858779 None:intergenic
AAACAATTCACACACAACAA+TGG 0.601217 5:+56858856 MsG0580027241.01.T01:exon
AACCCCGACGGCAACAACAC+CGG 0.607226 5:+56858976 MsG0580027241.01.T01:CDS
AGCAGATTCCGACACAGCAC+CGG 0.609241 5:+56859513 MsG0580027241.01.T01:CDS
TGTTGCCTTTACTGGTGCAG+GGG 0.612199 5:-56859266 MsG0580027240.01.T01:CDS
GACCGGTGTTGTTGCCGTCG+GGG 0.612817 5:-56858978 MsG0580027240.01.T01:CDS
GTTGCCGTCGGGGTTGTAGG+TGG 0.615116 5:-56858968 MsG0580027240.01.T01:CDS
CGTATGTAGTGGATCATCTG+TGG 0.615371 5:-56858768 None:intergenic
TGCACCAGTAAAGGCAACAC+CGG 0.624106 5:+56859270 MsG0580027241.01.T01:CDS
TGAATCCTAGCAGGCAACTG+AGG 0.626656 5:-56858910 MsG0580027240.01.T01:CDS
TGTAGTGGATCATCTGTGGA+AGG 0.633308 5:-56858764 None:intergenic
TATATTACCAAATTAAGACT+AGG 0.639002 5:-56859544 MsG0580027240.01.T01:intron
ACCATCAAATCATCTTCAAG+GGG 0.646558 5:+56859722 MsG0580027241.01.T01:CDS
TATTCATTGATCATCTAACA+AGG 0.656053 5:-56858711 None:intergenic
GGACTCTTGTGAATAACGGT+CGG 0.658554 5:-56859466 MsG0580027240.01.T01:intron
AGTGGATCATCTGTGGAAGG+AGG 0.682629 5:-56858761 None:intergenic
TTAGGTGCCACAGTAACAGG+TGG 0.683832 5:-56859049 MsG0580027240.01.T01:CDS
GTTGCCACCTACAACCCCGA+CGG 0.703871 5:+56858964 MsG0580027241.01.T01:CDS
CCATCAAATCATCTTCAAGG+GGG 0.727590 5:+56859723 MsG0580027241.01.T01:CDS
GTTGGTGGTGATGCAACAGG+TGG 0.762695 5:-56859196 MsG0580027240.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATAAATATATATAT+TGG + Chr5:56859592-56859611 MsG0580027241.01.T01:intron 0.0%
!!! AAAATTTGTTTCTTATTTAT+TGG - Chr5:56858821-56858840 None:intergenic 10.0%
!! ATTGTAATTTGATATTTCAA+TGG - Chr5:56859575-56859594 MsG0580027240.01.T01:intron 15.0%
!! TATATTACCAAATTAAGACT+AGG - Chr5:56859547-56859566 MsG0580027240.01.T01:intron 20.0%
! ATAATGTCACAATCAAATGA+AGG + Chr5:56858731-56858750 MsG0580027241.01.T01:five_prime_UTR 25.0%
! ATTCACTCTAAGCATTTATT+TGG + Chr5:56859835-56859854 MsG0580027241.01.T01:three_prime_UTR 25.0%
! GCAAAATGTAAAATCATTTC+TGG + Chr5:56859861-56859880 MsG0580027241.01.T01:three_prime_UTR 25.0%
! TAATGTCACAATCAAATGAA+GGG + Chr5:56858732-56858751 MsG0580027241.01.T01:five_prime_UTR 25.0%
! TATTCATTGATCATCTAACA+AGG - Chr5:56858714-56858733 None:intergenic 25.0%
! TTAACTAATGGAAAATGTGT+AGG - Chr5:56858687-56858706 None:intergenic 25.0%
! TTCACTCTAAGCATTTATTT+GGG + Chr5:56859836-56859855 MsG0580027241.01.T01:three_prime_UTR 25.0%
!! AATTTTGAATCATGGAAATC+AGG + Chr5:56859972-56859991 MsG0580027241.01.T01:three_prime_UTR 25.0%
!! GGATATGAAATTTTGAATCA+TGG + Chr5:56859964-56859983 MsG0580027241.01.T01:three_prime_UTR 25.0%
!!! ATGTTTTTTACTTCACAGAA+TGG + Chr5:56859696-56859715 MsG0580027241.01.T01:intron 25.0%
AAACAATTCACACACAACAA+TGG + Chr5:56858856-56858875 MsG0580027241.01.T01:exon 30.0%
ACATGTGTTCGTATTAGATT+TGG - Chr5:56859646-56859665 MsG0580027240.01.T01:intron 30.0%
GGAAATATATGGACAACAAT+TGG + Chr5:56859744-56859763 MsG0580027241.01.T01:CDS 30.0%
TAAAAATGCCGATAGTTATG+AGG + Chr5:56860005-56860024 MsG0580027241.01.T01:three_prime_UTR 30.0%
TAACAAGGAACGTTAACTAA+TGG - Chr5:56858699-56858718 None:intergenic 30.0%
TATTTGTCATGATCTTTGCT+CGG + Chr5:56859931-56859950 MsG0580027241.01.T01:three_prime_UTR 30.0%
!! TACTGTTTTTCTAGCAGTAA+CGG + Chr5:56859776-56859795 MsG0580027241.01.T01:CDS 30.0%
AACAATGGCTACAATGTTCT+GGG + Chr5:56858871-56858890 MsG0580027241.01.T01:exon 35.0%
ACCATCAAATCATCTTCAAG+GGG + Chr5:56859722-56859741 MsG0580027241.01.T01:CDS 35.0%
ATCTTCAAGGGGGAAATATA+TGG + Chr5:56859733-56859752 MsG0580027241.01.T01:CDS 35.0%
ATTTGTCATGATCTTTGCTC+GGG + Chr5:56859932-56859951 MsG0580027241.01.T01:three_prime_UTR 35.0%
CACCATCAAATCATCTTCAA+GGG + Chr5:56859721-56859740 MsG0580027241.01.T01:CDS 35.0%
!! ACTGTTTTTCTAGCAGTAAC+GGG + Chr5:56859777-56859796 MsG0580027241.01.T01:CDS 35.0%
ACCAAATTAAGACTAGGTGC+TGG - Chr5:56859541-56859560 MsG0580027240.01.T01:intron 40.0%
ATGGGTAGAAACGTATGTAG+TGG - Chr5:56858782-56858801 None:intergenic 40.0%
CAACAATGGCTACAATGTTC+TGG + Chr5:56858870-56858889 MsG0580027241.01.T01:exon 40.0%
CCATCAAATCATCTTCAAGG+GGG + Chr5:56859723-56859742 MsG0580027241.01.T01:CDS 40.0%
GATGTAACTGGAGCAGATTT+AGG - Chr5:56859070-56859089 MsG0580027240.01.T01:intron 40.0%
GCACCATCAAATCATCTTCA+AGG + Chr5:56859720-56859739 MsG0580027241.01.T01:CDS 40.0%
GGCTATATGATCAAGCAGAT+GGG - Chr5:56858800-56858819 None:intergenic 40.0%
TCTTTGCTCGGGAACATTAA+TGG + Chr5:56859943-56859962 MsG0580027241.01.T01:three_prime_UTR 40.0%
TGGCTATATGATCAAGCAGA+TGG - Chr5:56858801-56858820 None:intergenic 40.0%
! GTTGAGACAGGTGAACTTTT+TGG - Chr5:56859148-56859167 MsG0580027240.01.T01:intron 40.0%
!! ACCAGCACCTAGTCTTAATT+TGG + Chr5:56859537-56859556 MsG0580027241.01.T01:CDS 40.0%
AACTTTGTTGGTGCAGTTGC+TGG - Chr5:56858947-56858966 MsG0580027240.01.T01:CDS 45.0%
AGGGGGACTCTTGTGAATAA+CGG - Chr5:56859473-56859492 MsG0580027240.01.T01:intron 45.0%
AGTTACATCACCAGCTCCAA+AGG + Chr5:56859081-56859100 MsG0580027241.01.T01:CDS 45.0%
CCCCCTTGAAGATGATTTGA+TGG - Chr5:56859726-56859745 MsG0580027240.01.T01:intron 45.0%
GAATCTGCTGTAGTGTCAGT+TGG - Chr5:56859502-56859521 MsG0580027240.01.T01:CDS 45.0%
GGACTCTTGTGAATAACGGT+CGG - Chr5:56859469-56859488 MsG0580027240.01.T01:intron 45.0%
GTGTCTTCTTCTCTTGTGCT+TGG - Chr5:56859407-56859426 MsG0580027240.01.T01:intron 45.0%
GTTGTAGGTGGCAACTTTGT+TGG - Chr5:56858959-56858978 MsG0580027240.01.T01:CDS 45.0%
TCTTCTTCTCTTGTGCTTGG+AGG - Chr5:56859404-56859423 MsG0580027240.01.T01:intron 45.0%
! GAGACAGGTGAACTTTTTGG+TGG - Chr5:56859145-56859164 MsG0580027240.01.T01:intron 45.0%
! GAGACTTTTGGACTTGAAGC+TGG - Chr5:56859112-56859131 MsG0580027240.01.T01:intron 45.0%
! GATTTAGGTGCCACAGTAAC+AGG - Chr5:56859055-56859074 MsG0580027240.01.T01:intron 45.0%
! TTTGGAGCTGGTGATGTAAC+TGG - Chr5:56859082-56859101 MsG0580027240.01.T01:intron 45.0%
!! ACTGGTGTTGGCGATATCTT+TGG - Chr5:56859232-56859251 MsG0580027240.01.T01:CDS 45.0%
!! ATCTTTGGTGGTGGTAACGT+TGG - Chr5:56859217-56859236 MsG0580027240.01.T01:CDS 45.0%
!! CGTATGTAGTGGATCATCTG+TGG - Chr5:56858771-56858790 None:intergenic 45.0%
!! GAACTTTTTGGTGGTTGAGG+TGG - Chr5:56859136-56859155 MsG0580027240.01.T01:intron 45.0%
!! GGTGAACTTTTTGGTGGTTG+AGG - Chr5:56859139-56859158 MsG0580027240.01.T01:intron 45.0%
!! TGTAGTGGATCATCTGTGGA+AGG - Chr5:56858767-56858786 None:intergenic 45.0%
!!! AACAGGTGGTTGGCTTTTGA+TGG - Chr5:56859038-56859057 MsG0580027240.01.T01:CDS 45.0%
!!! ACAGGTGGTTGGCTTTTGAT+GGG - Chr5:56859037-56859056 MsG0580027240.01.T01:CDS 45.0%
AAGCCAACCACCTGTTACTG+TGG + Chr5:56859042-56859061 MsG0580027241.01.T01:CDS 50.0%
AGGAGTTGGCGCTTGCTTTA+CGG - Chr5:56859311-56859330 MsG0580027240.01.T01:CDS 50.0%
AGTGGATCATCTGTGGAAGG+AGG - Chr5:56858764-56858783 None:intergenic 50.0%
CTTGAAGCTGGTGCAACCTT+TGG - Chr5:56859100-56859119 MsG0580027240.01.T01:intron 50.0%
GAAGACACAGACACAAGCAC+AGG + Chr5:56859419-56859438 MsG0580027241.01.T01:CDS 50.0%
GGCAATGTTGGAGTTGAGAC+AGG - Chr5:56859160-56859179 MsG0580027240.01.T01:CDS 50.0%
GGTACAGGTGCTTCTATTGC+AGG - Chr5:56859370-56859389 MsG0580027240.01.T01:CDS 50.0%
GTGTTGCCTTTACTGGTGCA+GGG - Chr5:56859270-56859289 MsG0580027240.01.T01:CDS 50.0%
GTTGGCGATATCTTTGGTGG+TGG - Chr5:56859226-56859245 MsG0580027240.01.T01:CDS 50.0%
TCTGCTGTAGTGTCAGTTGG+TGG - Chr5:56859499-56859518 MsG0580027240.01.T01:CDS 50.0%
TGAATCCTAGCAGGCAACTG+AGG - Chr5:56858913-56858932 MsG0580027240.01.T01:CDS 50.0%
TGCACCAGTAAAGGCAACAC+CGG + Chr5:56859270-56859289 MsG0580027241.01.T01:CDS 50.0%
TGTTGCCTTTACTGGTGCAG+GGG - Chr5:56859269-56859288 MsG0580027240.01.T01:CDS 50.0%
TTGTGAATAACGGTCGGTGC+TGG - Chr5:56859463-56859482 MsG0580027240.01.T01:intron 50.0%
! GGTTGAGGTGGTGAGACTTT+TGG - Chr5:56859124-56859143 MsG0580027240.01.T01:intron 50.0%
! TTAGGTGCCACAGTAACAGG+TGG - Chr5:56859052-56859071 MsG0580027240.01.T01:CDS 50.0%
!! AACGTTGGTGGTGATGCAAC+AGG - Chr5:56859202-56859221 MsG0580027240.01.T01:CDS 50.0%
!! GGTGTTGGCGATATCTTTGG+TGG - Chr5:56859229-56859248 MsG0580027240.01.T01:CDS 50.0%
!! TAGTGTCAGTTGGTGGTGCA+GGG - Chr5:56859492-56859511 MsG0580027240.01.T01:intron 50.0%
!! TTAAGACTAGGTGCTGGTGC+CGG - Chr5:56859535-56859554 MsG0580027240.01.T01:intron 50.0%
!! TTCTATTGCAGGCGTTGGTG+CGG - Chr5:56859359-56859378 MsG0580027240.01.T01:CDS 50.0%
!! TTTGGTGGTGGTAACGTTGG+TGG - Chr5:56859214-56859233 MsG0580027240.01.T01:CDS 50.0%
AGCAGATTCCGACACAGCAC+CGG + Chr5:56859513-56859532 MsG0580027241.01.T01:CDS 55.0%
CTAAGCCTCAGTTGCCTGCT+AGG + Chr5:56858905-56858924 MsG0580027241.01.T01:CDS 55.0%
GATGGGAGGAGATGCTGCAA+CGG - Chr5:56859020-56859039 MsG0580027240.01.T01:CDS 55.0%
GGAGTTGGCGCTTGCTTTAC+GGG - Chr5:56859310-56859329 MsG0580027240.01.T01:CDS 55.0%
GGTGCTTCTATTGCAGGCGT+TGG - Chr5:56859364-56859383 MsG0580027240.01.T01:CDS 55.0%
GTCGGTGGTTGTGTCATGAC+CGG - Chr5:56858998-56859017 MsG0580027240.01.T01:CDS 55.0%
GTGCCACAGTAACAGGTGGT+TGG - Chr5:56859048-56859067 MsG0580027240.01.T01:CDS 55.0%
TGGTGCAGTTGCTGGTGCTT+GGG - Chr5:56858939-56858958 MsG0580027240.01.T01:CDS 55.0%
TTGGTGCAGTTGCTGGTGCT+TGG - Chr5:56858940-56858959 MsG0580027240.01.T01:CDS 55.0%
! GGTGTTGCCTTTACTGGTGC+AGG - Chr5:56859271-56859290 MsG0580027240.01.T01:CDS 55.0%
!! AGTGTCAGTTGGTGGTGCAG+GGG - Chr5:56859491-56859510 MsG0580027240.01.T01:intron 55.0%
!! ATGACCGGTGTTGTTGCCGT+CGG - Chr5:56858983-56859002 MsG0580027240.01.T01:CDS 55.0%
!! CTTGGGCGTTGAATCCTAGC+AGG - Chr5:56858922-56858941 MsG0580027240.01.T01:CDS 55.0%
!! GGTGGTGTTTGGACTGGTGT+TGG - Chr5:56859244-56859263 MsG0580027240.01.T01:CDS 55.0%
!! GTAGTGTCAGTTGGTGGTGC+AGG - Chr5:56859493-56859512 MsG0580027240.01.T01:intron 55.0%
!! GTTGGTGGTGATGCAACAGG+TGG - Chr5:56859199-56859218 MsG0580027240.01.T01:CDS 55.0%
!! TCTATTGCAGGCGTTGGTGC+GGG - Chr5:56859358-56859377 MsG0580027240.01.T01:CDS 55.0%
!!! GGTGGTTGGCTTTTGATGGG+AGG - Chr5:56859034-56859053 MsG0580027240.01.T01:CDS 55.0%
AACCCCGACGGCAACAACAC+CGG + Chr5:56858976-56858995 MsG0580027241.01.T01:CDS 60.0%
ACCAGCCCCTGCACCAGTAA+AGG + Chr5:56859261-56859280 MsG0580027241.01.T01:CDS 60.0%
CTCTTGTGCTTGGAGGACGC+AGG - Chr5:56859397-56859416 MsG0580027240.01.T01:intron 60.0%
GGAGGAGATGCTGCAACGGT+CGG - Chr5:56859016-56859035 MsG0580027240.01.T01:CDS 60.0%
GGAGTAGCAGGTGCAGGAGT+TGG - Chr5:56859325-56859344 MsG0580027240.01.T01:CDS 60.0%
GTTGCCACCTACAACCCCGA+CGG + Chr5:56858964-56858983 MsG0580027241.01.T01:CDS 60.0%
GTTGTTGCCGTCGGGGTTGT+AGG - Chr5:56858974-56858993 MsG0580027240.01.T01:CDS 60.0%
TGCTTTACGGGTGCAGGTGC+TGG - Chr5:56859298-56859317 MsG0580027240.01.T01:CDS 60.0%
TTTACTGGTGCAGGGGCTGG+TGG - Chr5:56859262-56859281 MsG0580027240.01.T01:CDS 60.0%
! GGCAACGGTGGTGGCAATGT+TGG - Chr5:56859172-56859191 MsG0580027240.01.T01:CDS 60.0%
! GGTGATGCAACAGGTGGTGG+TGG - Chr5:56859193-56859212 MsG0580027240.01.T01:CDS 60.0%
! GGTGGTGATGCAACAGGTGG+TGG - Chr5:56859196-56859215 MsG0580027240.01.T01:CDS 60.0%
!! ATAACGGTCGGTGCTGGAGC+TGG - Chr5:56859457-56859476 MsG0580027240.01.T01:intron 60.0%
!! GCAACAGGTGGTGGTGGCAA+CGG - Chr5:56859187-56859206 MsG0580027240.01.T01:CDS 60.0%
!! GCTGGTGCAACCTTTGGAGC+TGG - Chr5:56859094-56859113 MsG0580027240.01.T01:intron 60.0%
!! GGCACCGGTGTTGCCTTTAC+TGG - Chr5:56859277-56859296 MsG0580027240.01.T01:CDS 60.0%
!! GGTATTGGTGGCGGAGTAGC+AGG - Chr5:56859337-56859356 MsG0580027240.01.T01:CDS 60.0%
!! GTGTCAGTTGGTGGTGCAGG+GGG - Chr5:56859490-56859509 MsG0580027240.01.T01:intron 60.0%
!! GTTGGTGCGGGTATTGGTGG+CGG - Chr5:56859346-56859365 MsG0580027240.01.T01:CDS 60.0%
!! TGACCGGTGTTGTTGCCGTC+GGG - Chr5:56858982-56859001 MsG0580027240.01.T01:CDS 60.0%
GCCTTTACTGGTGCAGGGGC+TGG - Chr5:56859265-56859284 MsG0580027240.01.T01:CDS 65.0%
GGAGATGCTGCAACGGTCGG+TGG - Chr5:56859013-56859032 MsG0580027240.01.T01:CDS 65.0%
GGCGCTTGCTTTACGGGTGC+AGG - Chr5:56859304-56859323 MsG0580027240.01.T01:CDS 65.0%
GTTGCCGTCGGGGTTGTAGG+TGG - Chr5:56858971-56858990 MsG0580027240.01.T01:CDS 65.0%
TGCTTGGAGGACGCAGGTGC+TGG - Chr5:56859391-56859410 MsG0580027240.01.T01:intron 65.0%
! GGGGCTGGTGGTGTTTGGAC+TGG - Chr5:56859250-56859269 MsG0580027240.01.T01:CDS 65.0%
! GTGCAGGGGCTGGTGGTGTT+TGG - Chr5:56859255-56859274 MsG0580027240.01.T01:CDS 65.0%
!! ACAGGTGGTGGTGGCAACGG+TGG - Chr5:56859184-56859203 MsG0580027240.01.T01:CDS 65.0%
!! GACCGGTGTTGTTGCCGTCG+GGG - Chr5:56858981-56859000 MsG0580027240.01.T01:CDS 65.0%
!! GAGGACGCAGGTGCTGGTAC+AGG - Chr5:56859385-56859404 MsG0580027240.01.T01:intron 65.0%
!! GCAGGCGTTGGTGCGGGTAT+TGG - Chr5:56859352-56859371 MsG0580027240.01.T01:CDS 65.0%
!! GGCGTTGGTGCGGGTATTGG+TGG - Chr5:56859349-56859368 MsG0580027240.01.T01:CDS 65.0%
!! TGCTGGTGCCGGTGCTGTGT+CGG - Chr5:56859524-56859543 MsG0580027240.01.T01:CDS 65.0%
!! ACGGGTGCAGGTGCTGGCAC+CGG - Chr5:56859292-56859311 MsG0580027240.01.T01:CDS 70.0%
!! GGTGGCGGAGTAGCAGGTGC+AGG - Chr5:56859331-56859350 MsG0580027240.01.T01:CDS 70.0%
!! GGTGGTGGTGGCAACGGTGG+TGG - Chr5:56859181-56859200 MsG0580027240.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 56858664 56860026 56858664 ID=MsG0580027241.01;Name=MsG0580027241.01
Chr5 mRNA 56858664 56860026 56858664 ID=MsG0580027241.01.T01;Parent=MsG0580027241.01;Name=MsG0580027241.01.T01;_AED=0.08;_eAED=0.08;_QI=211|1|1|1|0|0|2|214|260
Chr5 exon 56858664 56859558 56858664 ID=MsG0580027241.01.T01:exon:12769;Parent=MsG0580027241.01.T01
Chr5 exon 56859714 56860026 56859714 ID=MsG0580027241.01.T01:exon:12770;Parent=MsG0580027241.01.T01
Chr5 five_prime_UTR 56858664 56858874 56858664 ID=MsG0580027241.01.T01:five_prime_utr;Parent=MsG0580027241.01.T01
Chr5 CDS 56858875 56859558 56858875 ID=MsG0580027241.01.T01:cds;Parent=MsG0580027241.01.T01
Chr5 CDS 56859714 56859812 56859714 ID=MsG0580027241.01.T01:cds;Parent=MsG0580027241.01.T01
Chr5 three_prime_UTR 56859813 56860026 56859813 ID=MsG0580027241.01.T01:three_prime_utr;Parent=MsG0580027241.01.T01
Gene Sequence

>MsG0580027241.01.T01

ATGGCTACAATGTTCTGGGTGATGACTTTACTAAGCCTCAGTTGCCTGCTAGGATTCAACGCCCAAGCACCAGCAACTGCACCAACAAAGTTGCCACCTACAACCCCGACGGCAACAACACCGGTCATGACACAACCACCGACCGTTGCAGCATCTCCTCCCATCAAAAGCCAACCACCTGTTACTGTGGCACCTAAATCTGCTCCAGTTACATCACCAGCTCCAAAGGTTGCACCAGCTTCAAGTCCAAAAGTCTCACCACCTCAACCACCAAAAAGTTCACCTGTCTCAACTCCAACATTGCCACCACCGTTGCCACCACCACCTGTTGCATCACCACCAACGTTACCACCACCAAAGATATCGCCAACACCAGTCCAAACACCACCAGCCCCTGCACCAGTAAAGGCAACACCGGTGCCAGCACCTGCACCCGTAAAGCAAGCGCCAACTCCTGCACCTGCTACTCCGCCACCAATACCCGCACCAACGCCTGCAATAGAAGCACCTGTACCAGCACCTGCGTCCTCCAAGCACAAGAGAAGAAGACACAGACACAAGCACAGGAGACATCAAGCACCAGCTCCAGCACCGACCGTTATTCACAAGAGTCCCCCTGCACCACCAACTGACACTACAGCAGATTCCGACACAGCACCGGCACCAGCACCTAGTCTTAATTTGAATGGTGCACCATCAAATCATCTTCAAGGGGGAAATATATGGACAACAATTGGATTTGCTATTACTGTTTTTCTAGCAGTAACGGGCTACAGCTGCTAG
Protein sequence

>MsG0580027241.01.T01

MATMFWVMTLLSLSCLLGFNAQAPATAPTKLPPTTPTATTPVMTQPPTVAASPPIKSQPPVTVAPKSAPVTSPAPKVAPASSPKVSPPQPPKSSPVSTPTLPPPLPPPPVASPPTLPPPKISPTPVQTPPAPAPVKATPVPAPAPVKQAPTPAPATPPPIPAPTPAIEAPVPAPASSKHKRRRHRHKHRRHQAPAPAPTVIHKSPPAPPTDTTADSDTAPAPAPSLNLNGAPSNHLQGGNIWTTIGFAITVFLAVTGYSC*