AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028470.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028470.01.T03 MTR_5g072400 92.424 264 7 2 1 254 1 261 4.68e-161 447
MsG0580028470.01.T03 MTR_3g465550 54.618 249 85 5 1 242 1 228 1.48e-80 242
MsG0580028470.01.T03 MTR_5g083110 46.154 260 106 7 1 247 1 239 3.65e-67 208
MsG0580028470.01.T03 MTR_3g071510 39.111 225 109 4 25 247 37 235 7.09e-47 156
MsG0580028470.01.T03 MTR_3g017490 46.067 178 84 6 78 246 86 260 2.15e-45 154
MsG0580028470.01.T03 MTR_8g038440 37.200 250 137 4 13 246 9 254 3.16e-45 152
MsG0580028470.01.T03 MTR_7g090230 49.342 152 73 3 97 246 275 424 7.15e-45 156
MsG0580028470.01.T03 MTR_1g051180 48.447 161 69 3 97 246 206 363 7.54e-44 152
MsG0580028470.01.T03 MTR_1g090400 43.046 151 81 2 97 246 222 368 6.37e-35 129
MsG0580028470.01.T03 MTR_7g104720 43.046 151 81 3 97 246 258 404 4.16e-33 124
MsG0580028470.01.T03 MTR_6g011640 40.244 164 81 2 100 246 233 396 1.89e-32 122
MsG0580028470.01.T03 MTR_7g010090 44.186 172 70 4 97 246 264 431 2.20e-30 117
MsG0580028470.01.T03 MTR_8g099665 38.636 176 96 4 78 247 199 368 1.58e-28 111
MsG0580028470.01.T03 MTR_7g106500 37.500 160 86 3 97 246 123 278 3.01e-28 108
MsG0580028470.01.T03 MTR_8g106900 43.871 155 77 4 99 246 122 273 1.88e-25 101
MsG0580028470.01.T03 MTR_3g030520 37.975 158 81 2 106 246 141 298 5.46e-24 98.2
MsG0580028470.01.T03 MTR_5g048870 40.000 90 54 0 99 188 68 157 2.83e-13 66.2
MsG0580028470.01.T02 MTR_5g072400 85.227 264 4 2 1 232 1 261 2.18e-161 447
MsG0580028470.01.T02 MTR_3g465550 54.545 242 74 5 1 220 1 228 2.79e-77 233
MsG0580028470.01.T02 MTR_5g083110 46.964 247 101 6 1 225 1 239 1.16e-68 211
MsG0580028470.01.T02 MTR_3g071510 43.137 204 108 4 25 225 37 235 1.27e-50 165
MsG0580028470.01.T02 MTR_8g038440 48.026 152 77 2 75 224 103 254 4.92e-45 151
MsG0580028470.01.T02 MTR_7g090230 49.342 152 73 3 75 224 275 424 5.74e-45 155
MsG0580028470.01.T02 MTR_3g017490 48.344 151 73 3 76 224 113 260 6.57e-45 152
MsG0580028470.01.T02 MTR_1g051180 48.447 161 69 3 75 224 206 363 5.33e-44 151
MsG0580028470.01.T02 MTR_1g090400 43.226 155 83 2 71 224 218 368 5.47e-36 130
MsG0580028470.01.T02 MTR_6g011640 33.180 217 124 3 25 224 184 396 9.72e-34 125
MsG0580028470.01.T02 MTR_7g104720 43.226 155 83 3 71 224 254 404 9.75e-34 125
MsG0580028470.01.T02 MTR_7g010090 44.186 172 70 4 75 224 264 431 1.85e-30 117
MsG0580028470.01.T02 MTR_8g099665 40.491 163 91 3 69 225 206 368 2.09e-29 113
MsG0580028470.01.T02 MTR_7g106500 37.805 164 88 3 71 224 119 278 2.10e-29 111
MsG0580028470.01.T02 MTR_8g106900 43.396 159 80 4 73 224 118 273 3.69e-26 102
MsG0580028470.01.T02 MTR_3g030520 37.975 158 81 2 84 224 141 298 2.59e-24 98.6
MsG0580028470.01.T02 MTR_5g048870 39.362 94 57 0 73 166 64 157 3.95e-14 68.2
MsG0580028470.01.T01 MTR_5g072400 86.742 264 5 2 1 237 1 261 3.65e-165 456
MsG0580028470.01.T01 MTR_3g465550 53.306 242 82 5 1 225 1 228 3.94e-75 227
MsG0580028470.01.T01 MTR_5g083110 46.586 249 104 8 1 230 1 239 8.10e-66 204
MsG0580028470.01.T01 MTR_3g071510 42.718 206 111 3 25 230 37 235 8.57e-50 163
MsG0580028470.01.T01 MTR_8g038440 36.614 254 116 4 13 229 9 254 1.44e-46 155
MsG0580028470.01.T01 MTR_3g017490 45.455 176 88 5 59 229 88 260 7.92e-46 154
MsG0580028470.01.T01 MTR_7g090230 49.342 152 73 3 80 229 275 424 5.89e-45 155
MsG0580028470.01.T01 MTR_1g051180 48.447 161 69 3 80 229 206 363 6.47e-44 151
MsG0580028470.01.T01 MTR_1g090400 40.588 170 86 3 61 229 213 368 1.19e-35 130
MsG0580028470.01.T01 MTR_6g011640 32.432 222 124 3 25 229 184 396 5.79e-33 123
MsG0580028470.01.T01 MTR_7g104720 43.046 151 81 3 80 229 258 404 7.05e-33 123
MsG0580028470.01.T01 MTR_7g010090 44.186 172 70 4 80 229 264 431 2.64e-30 117
MsG0580028470.01.T01 MTR_8g099665 41.447 152 83 3 85 230 217 368 2.17e-28 110
MsG0580028470.01.T01 MTR_7g106500 37.500 160 86 3 80 229 123 278 2.40e-28 108
MsG0580028470.01.T01 MTR_8g106900 43.871 155 77 4 82 229 122 273 1.89e-25 101
MsG0580028470.01.T01 MTR_3g030520 37.975 158 81 2 89 229 141 298 4.27e-24 98.2
MsG0580028470.01.T01 MTR_5g048870 40.000 90 54 0 82 171 68 157 2.15e-13 66.2
MsG0580028470.01.T04 MTR_5g072400 95.652 115 2 1 1 115 150 261 2.60e-77 229
MsG0580028470.01.T04 MTR_3g465550 61.165 103 38 1 1 103 128 228 1.70e-40 134
MsG0580028470.01.T04 MTR_5g083110 50.926 108 50 1 1 108 135 239 6.74e-32 112
MsG0580028470.01.T04 MTR_3g071510 45.370 108 56 1 1 108 131 235 2.46e-29 106
MsG0580028470.01.T04 MTR_7g090230 47.706 109 53 3 1 107 318 424 8.81e-28 105
MsG0580028470.01.T04 MTR_3g017490 46.789 109 53 3 1 107 155 260 2.50e-27 102
MsG0580028470.01.T04 MTR_1g051180 45.763 118 50 3 1 107 249 363 2.60e-25 97.8
MsG0580028470.01.T04 MTR_8g038440 43.119 109 60 2 1 107 146 254 1.00e-23 92.0
MsG0580028470.01.T04 MTR_1g090400 42.593 108 57 2 1 107 265 368 8.81e-22 88.6
MsG0580028470.01.T04 MTR_7g104720 39.815 108 60 3 1 107 301 404 3.50e-17 75.9
MsG0580028470.01.T04 MTR_6g011640 37.903 124 60 2 1 107 273 396 6.20e-17 75.1
MsG0580028470.01.T04 MTR_8g099665 43.011 93 49 2 20 108 276 368 5.73e-15 69.7
MsG0580028470.01.T04 MTR_7g106500 35.897 117 61 3 1 107 166 278 1.73e-14 67.8
MsG0580028470.01.T04 MTR_7g010090 42.453 106 36 3 24 107 329 431 2.54e-14 67.8
MsG0580028470.01.T04 MTR_3g030520 35.484 124 63 2 1 107 175 298 3.79e-13 64.3
MsG0580028470.01.T04 MTR_8g106900 41.228 114 57 4 1 107 163 273 4.80e-12 60.8
MsG0580028470.01.T04 MTR_2g026830 36.471 85 53 1 1 84 1 85 1.41e-11 57.0
MsG0580028470.01.T04 MTR_7g079015 35.955 89 52 1 1 84 1 89 3.25e-11 55.8
MsG0580028470.01.T04 MTR_1g095960 36.471 85 49 1 5 84 32 116 7.59e-11 55.8
MsG0580028470.01.T04 MTR_1g098230 37.209 86 49 1 6 86 6 91 9.70e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028470.01.T03 AT5G58530 44.928 276 120 7 1 250 1 270 6.18e-74 226
MsG0580028470.01.T03 AT5G13810 46.154 182 91 4 69 246 94 272 5.44e-47 157
MsG0580028470.01.T03 AT2G41330 45.912 159 82 3 90 246 244 400 1.99e-40 144
MsG0580028470.01.T03 AT3G57070 45.625 160 83 4 89 246 258 415 1.49e-39 142
MsG0580028470.01.T03 AT5G39865 43.030 165 77 4 99 246 224 388 3.53e-37 135
MsG0580028470.01.T03 AT3G28850 40.113 177 77 3 99 246 250 426 7.83e-34 127
MsG0580028470.01.T03 AT5G06470 41.772 158 79 4 97 246 86 238 4.01e-33 120
MsG0580028470.01.T03 AT3G11773 40.136 147 83 2 101 246 7 149 3.08e-31 113
MsG0580028470.01.T03 AT5G01420 38.000 150 91 1 97 246 253 400 2.96e-30 117
MsG0580028470.01.T03 AT4G10630 38.889 198 101 6 69 246 136 333 4.17e-30 115
MsG0580028470.01.T03 AT1G64500 36.313 179 97 4 80 249 198 368 1.16e-29 114
MsG0580028470.01.T03 AT1G32760 44.231 156 81 2 97 246 158 313 5.88e-29 111
MsG0580028470.01.T03 AT5G03870 43.137 153 81 3 97 246 234 383 1.27e-27 109
MsG0580028470.01.T03 AT3G11773 41.071 112 62 1 135 246 92 199 5.56e-24 95.9
MsG0580028470.01.T02 AT5G58530 43.590 273 106 6 1 228 1 270 2.03e-70 217
MsG0580028470.01.T02 AT5G13810 44.118 204 103 6 27 224 74 272 1.76e-48 160
MsG0580028470.01.T02 AT2G41330 47.059 153 77 3 74 224 250 400 3.30e-40 142
MsG0580028470.01.T02 AT3G57070 46.154 156 80 4 71 224 262 415 1.45e-39 141
MsG0580028470.01.T02 AT5G39865 43.030 165 77 4 77 224 224 388 4.40e-37 134
MsG0580028470.01.T02 AT5G06470 41.875 160 80 4 73 224 84 238 4.36e-34 122
MsG0580028470.01.T02 AT3G28850 40.113 177 77 3 77 224 250 426 7.82e-34 126
MsG0580028470.01.T02 AT3G11773 40.136 147 83 2 79 224 7 149 1.45e-31 113
MsG0580028470.01.T02 AT5G01420 37.662 154 94 1 71 224 249 400 5.23e-31 118
MsG0580028470.01.T02 AT1G64500 38.095 168 93 3 69 227 203 368 8.16e-31 117
MsG0580028470.01.T02 AT4G10630 42.331 163 83 4 73 224 171 333 1.72e-30 115
MsG0580028470.01.T02 AT1G32760 43.750 160 84 2 71 224 154 313 1.40e-29 112
MsG0580028470.01.T02 AT5G03870 43.396 159 84 3 69 224 228 383 3.94e-29 112
MsG0580028470.01.T02 AT3G11773 41.071 112 62 1 113 224 92 199 3.62e-24 95.9
MsG0580028470.01.T01 AT5G58530 43.590 273 111 6 1 233 1 270 2.07e-68 212
MsG0580028470.01.T01 AT5G13810 50.658 152 70 3 80 229 124 272 8.58e-47 156
MsG0580028470.01.T01 AT2G41330 45.912 159 82 3 73 229 244 400 2.40e-40 143
MsG0580028470.01.T01 AT3G57070 45.625 160 83 4 72 229 258 415 1.36e-39 141
MsG0580028470.01.T01 AT5G39865 43.030 165 77 4 82 229 224 388 3.63e-37 134
MsG0580028470.01.T01 AT3G28850 40.113 177 77 3 82 229 250 426 8.80e-34 126
MsG0580028470.01.T01 AT5G06470 41.772 158 79 4 80 229 86 238 1.87e-33 121
MsG0580028470.01.T01 AT3G11773 40.136 147 83 2 84 229 7 149 1.63e-31 113
MsG0580028470.01.T01 AT5G01420 38.000 150 91 1 80 229 253 400 3.22e-30 116
MsG0580028470.01.T01 AT4G10630 42.236 161 82 4 80 229 173 333 9.05e-30 114
MsG0580028470.01.T01 AT1G64500 37.654 162 90 3 80 232 209 368 1.75e-29 113
MsG0580028470.01.T01 AT1G32760 44.231 156 81 2 80 229 158 313 3.94e-29 111
MsG0580028470.01.T01 AT5G03870 43.137 153 81 3 80 229 234 383 1.68e-27 108
MsG0580028470.01.T01 AT3G11773 41.071 112 62 1 118 229 92 199 2.83e-24 96.3
MsG0580028470.01.T04 AT5G58530 53.390 118 48 2 1 111 153 270 3.41e-40 135
MsG0580028470.01.T04 AT5G13810 47.706 109 52 3 1 107 167 272 2.89e-27 101
MsG0580028470.01.T04 AT2G41330 46.789 109 54 3 1 107 294 400 2.00e-22 90.5
MsG0580028470.01.T04 AT3G57070 44.037 109 58 3 1 107 308 415 7.47e-22 89.0
MsG0580028470.01.T04 AT5G06470 40.000 115 56 4 1 107 129 238 8.05e-22 86.7
MsG0580028470.01.T04 AT3G11773 38.318 107 62 1 1 107 47 149 8.65e-22 84.3
MsG0580028470.01.T04 AT3G11773 38.318 107 62 1 1 107 97 199 1.52e-21 85.1
MsG0580028470.01.T04 AT5G39865 40.323 124 57 4 1 107 265 388 1.82e-20 85.1
MsG0580028470.01.T04 AT3G28850 38.971 136 54 3 1 107 291 426 2.16e-19 82.4
MsG0580028470.01.T04 AT1G64500 36.134 119 65 3 1 110 252 368 1.67e-17 76.6
MsG0580028470.01.T04 AT5G01420 34.579 107 68 1 1 107 296 400 3.84e-17 75.9
MsG0580028470.01.T04 AT4G10630 38.983 118 61 4 1 107 216 333 2.20e-15 70.9
MsG0580028470.01.T04 AT1G32760 40.708 113 61 2 1 107 201 313 2.61e-15 70.5
MsG0580028470.01.T04 AT5G03870 40.000 110 60 3 1 107 277 383 1.88e-12 62.8

Find 60 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTTCCCCGAAAATAAA+TGG 0.139719 5:+81000592 None:intergenic
GACCGAACTGAACCGGATTA+TGG 0.249579 5:-81000432 MsG0580028470.01.T03:CDS
CCCCGAAAATAAATGGGTTT+TGG 0.260046 5:+81000599 None:intergenic
GTAATGAGAATGGTCTTCTT+AGG 0.325866 5:-81000163 MsG0580028470.01.T03:CDS
CTTTCTTGACCGAACTGAAC+CGG 0.329290 5:-81000439 MsG0580028470.01.T03:CDS
ACCGAACTGAACCGGATTAT+GGG 0.342370 5:-81000431 MsG0580028470.01.T03:CDS
TTTATAGCTGGAAAATATAT+CGG 0.359281 5:-81000371 MsG0580028470.01.T03:CDS
GACCGGTTCGACCCATAATC+CGG 0.406930 5:+81000420 None:intergenic
GAGGTGGTTCTTGAAGAATA+AGG 0.408273 5:+81000774 None:intergenic
TTCTATGTAACGTGTGTAAT+GGG 0.410909 5:-81000235 MsG0580028470.01.T03:CDS
CAATGATAAACCGGTTTGAC+CGG 0.419092 5:+81000403 None:intergenic
GCACACACGTGGCATTCAAT+TGG 0.427039 5:+81000272 None:intergenic
AGGTGGTTCTTGAAGAATAA+GGG 0.429947 5:+81000775 None:intergenic
ATTAGATCAATGCACGAGAT+TGG 0.437076 5:-81000335 MsG0580028470.01.T03:CDS
ACCCATAATCCGGTTCAGTT+CGG 0.438094 5:+81000430 None:intergenic
ATGGGTCGAACCGGTCAAAC+CGG 0.443878 5:-81000413 MsG0580028470.01.T03:CDS
GTTCTATGTAACGTGTGTAA+TGG 0.447690 5:-81000236 MsG0580028470.01.T03:CDS
GTCATTGTGTGGGAGTTTGA+GGG 0.448123 5:+81000661 None:intergenic
CCAACACACTCCTCCGTGCC+TGG 0.451659 5:-81000691 MsG0580028470.01.T03:CDS
TTTGTTGTGGGTTGTGAAGA+AGG 0.454941 5:+81000740 None:intergenic
AACACGCGGCAATGATAAAC+CGG 0.461274 5:+81000394 None:intergenic
TCTGTTCCCCGAAAATAAAT+GGG 0.463765 5:+81000593 None:intergenic
GCCACGTGTGTGCTGGTCAT+AGG 0.468314 5:-81000262 MsG0580028470.01.T03:CDS
TGTCATTGTGTGGGAGTTTG+AGG 0.474316 5:+81000660 None:intergenic
AAGGGTTTGAATGTCTTTGA+AGG 0.484611 5:+81000793 None:intergenic
AACCGGATTATGGGTCGAAC+CGG 0.486019 5:-81000422 MsG0580028470.01.T03:CDS
GAGTTTGAGGGGAGATGACC+AGG 0.496090 5:+81000673 None:intergenic
TGTAATGTTTGTAATGAGAA+TGG 0.500646 5:-81000173 MsG0580028470.01.T03:CDS
TTGTTATTAGGAAAACAAGA+AGG 0.507938 5:+81000137 None:intergenic
CATTCAATTGGATCCACTAC+AGG 0.509106 5:+81000284 None:intergenic
AAGGTGTATAATGATAAAGC+TGG 0.523023 5:-81000206 MsG0580028470.01.T03:CDS
GTGGGTTGTGAAGAAGGTAG+TGG 0.534914 5:+81000746 None:intergenic
ATTGAATGCCACGTGTGTGC+TGG 0.535810 5:-81000269 MsG0580028470.01.T03:CDS
GCTAGAAGATTTGCCTGTAG+TGG 0.543192 5:-81000297 MsG0580028470.01.T03:CDS
GAAGGAAGAACAAGAGAATG+AGG 0.546720 5:+81000811 None:intergenic
CGACGAACGCGACGTGTCGA+TGG 0.555257 5:-81000468 MsG0580028470.01.T03:CDS
GAAGGTAGTGGTTGTTGAGG+TGG 0.558854 5:+81000758 None:intergenic
GGAGGAGTGTGTTGGCGAGA+AGG 0.560304 5:+81000699 None:intergenic
TCATTGTGTGGGAGTTTGAG+GGG 0.569756 5:+81000662 None:intergenic
ACATTCAATGTGGAAACCGT+GGG 0.573190 5:-81000873 None:intergenic
GGCGCGCGTGTGTCATTGTG+TGG 0.574993 5:+81000650 None:intergenic
CACATTCAATGTGGAAACCG+TGG 0.579707 5:-81000874 None:intergenic
GCTGGAAAATATATCGGTGG+AGG 0.585661 5:-81000365 MsG0580028470.01.T03:CDS
ACACAATGACACACGCGCGC+CGG 0.586530 5:-81000648 MsG0580028470.01.T03:CDS
TGTTGGCGAGAAGGACGCGT+TGG 0.588316 5:+81000708 None:intergenic
TTCGTCGAGATGAACGTGGA+AGG 0.588864 5:+81000484 None:intergenic
TGTTATTAGGAAAACAAGAA+GGG 0.589469 5:+81000138 None:intergenic
CGTGTGTAATGGGAGTAGAA+AGG 0.596593 5:-81000225 MsG0580028470.01.T03:CDS
CCAGGCACGGAGGAGTGTGT+TGG 0.613390 5:+81000691 None:intergenic
GATGAACGTGGAAGGCGCGT+AGG 0.618798 5:+81000492 None:intergenic
TGAGGGGAGATGACCAGGCA+CGG 0.630849 5:+81000678 None:intergenic
GCGCGCGTGTGTCATTGTGT+GGG 0.641651 5:+81000651 None:intergenic
GAAGAAGGTAGTGGTTGTTG+AGG 0.643694 5:+81000755 None:intergenic
ATACTTCACAAGCCTGCGCG+TGG 0.645281 5:-81000555 MsG0580028470.01.T03:CDS
AGATTGGTCGAACCACGCGC+AGG 0.645342 5:+81000543 None:intergenic
CGCGTTCGTCGAGATGAACG+TGG 0.649515 5:+81000480 None:intergenic
ACCTATGACCAGCACACACG+TGG 0.666756 5:+81000261 None:intergenic
ATAGCTGGAAAATATATCGG+TGG 0.674225 5:-81000368 MsG0580028470.01.T03:CDS
CATTCAATGTGGAAACCGTG+GGG 0.704505 5:-81000872 None:intergenic
GGGGAGATGACCAGGCACGG+AGG 0.758230 5:+81000681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGATTTCATTGTTATT+AGG + Chr5:81000865-81000884 None:intergenic 15.0%
!! TTTATAGCTGGAAAATATAT+CGG - Chr5:81000616-81000635 MsG0580028470.01.T03:CDS 20.0%
! TGTAATGTTTGTAATGAGAA+TGG - Chr5:81000814-81000833 MsG0580028470.01.T03:CDS 25.0%
! TGTTATTAGGAAAACAAGAA+GGG + Chr5:81000852-81000871 None:intergenic 25.0%
!! TTGTTATTAGGAAAACAAGA+AGG + Chr5:81000853-81000872 None:intergenic 25.0%
AAGGTGTATAATGATAAAGC+TGG - Chr5:81000781-81000800 MsG0580028470.01.T03:CDS 30.0%
TTCTATGTAACGTGTGTAAT+GGG - Chr5:81000752-81000771 MsG0580028470.01.T03:CDS 30.0%
!! CTCCAAAACCCATTTATTTT+CGG - Chr5:81000386-81000405 MsG0580028470.01.T03:CDS 30.0%
!! TCCAAAACCCATTTATTTTC+GGG - Chr5:81000387-81000406 MsG0580028470.01.T03:CDS 30.0%
!!! AAAATAAATGGGTTTTGGAG+GGG + Chr5:81000386-81000405 None:intergenic 30.0%
!!! GAAAATAAATGGGTTTTGGA+GGG + Chr5:81000387-81000406 None:intergenic 30.0%
AAGGGTTTGAATGTCTTTGA+AGG + Chr5:81000197-81000216 None:intergenic 35.0%
AGGTGGTTCTTGAAGAATAA+GGG + Chr5:81000215-81000234 None:intergenic 35.0%
ATAGCTGGAAAATATATCGG+TGG - Chr5:81000619-81000638 MsG0580028470.01.T03:CDS 35.0%
ATTAGATCAATGCACGAGAT+TGG - Chr5:81000652-81000671 MsG0580028470.01.T03:CDS 35.0%
GTAATGAGAATGGTCTTCTT+AGG - Chr5:81000824-81000843 MsG0580028470.01.T03:CDS 35.0%
GTTCTATGTAACGTGTGTAA+TGG - Chr5:81000751-81000770 MsG0580028470.01.T03:CDS 35.0%
TCTGTTCCCCGAAAATAAAT+GGG + Chr5:81000397-81000416 None:intergenic 35.0%
TTCTGTTCCCCGAAAATAAA+TGG + Chr5:81000398-81000417 None:intergenic 35.0%
TTTCCAGCTATAAAAACACG+CGG + Chr5:81000610-81000629 None:intergenic 35.0%
!! CCAAAACCCATTTATTTTCG+GGG - Chr5:81000388-81000407 MsG0580028470.01.T03:CDS 35.0%
!! CGAAAATAAATGGGTTTTGG+AGG + Chr5:81000388-81000407 None:intergenic 35.0%
!!! TGGAAGACTTGTTTTGTTGT+GGG + Chr5:81000262-81000281 None:intergenic 35.0%
!!! TTAGATGTTGATTTTCCCCA+CGG + Chr5:81000133-81000152 None:intergenic 35.0%
!!! TTGGAAGACTTGTTTTGTTG+TGG + Chr5:81000263-81000282 None:intergenic 35.0%
CATTCAATTGGATCCACTAC+AGG + Chr5:81000706-81000725 None:intergenic 40.0%
GAAGGAAGAACAAGAGAATG+AGG + Chr5:81000179-81000198 None:intergenic 40.0%
GAGGTGGTTCTTGAAGAATA+AGG + Chr5:81000216-81000235 None:intergenic 40.0%
! CTTTTGCAGTCTTCGAAGAT+TGG + Chr5:81000463-81000482 None:intergenic 40.0%
! TTTGTTGTGGGTTGTGAAGA+AGG + Chr5:81000250-81000269 None:intergenic 40.0%
!! CAATGATAAACCGGTTTGAC+CGG + Chr5:81000587-81000606 None:intergenic 40.0%
!! CCCCGAAAATAAATGGGTTT+TGG + Chr5:81000391-81000410 None:intergenic 40.0%
AACACGCGGCAATGATAAAC+CGG + Chr5:81000596-81000615 None:intergenic 45.0%
ACCCATAATCCGGTTCAGTT+CGG + Chr5:81000560-81000579 None:intergenic 45.0%
ACCGAACTGAACCGGATTAT+GGG - Chr5:81000556-81000575 MsG0580028470.01.T03:CDS 45.0%
CTTTCTTGACCGAACTGAAC+CGG - Chr5:81000548-81000567 MsG0580028470.01.T03:CDS 45.0%
GCTAGAAGATTTGCCTGTAG+TGG - Chr5:81000690-81000709 MsG0580028470.01.T03:CDS 45.0%
GCTGGAAAATATATCGGTGG+AGG - Chr5:81000622-81000641 MsG0580028470.01.T03:CDS 45.0%
GTCATTGTGTGGGAGTTTGA+GGG + Chr5:81000329-81000348 None:intergenic 45.0%
TCATTGTGTGGGAGTTTGAG+GGG + Chr5:81000328-81000347 None:intergenic 45.0%
TGTCATTGTGTGGGAGTTTG+AGG + Chr5:81000330-81000349 None:intergenic 45.0%
! CGTGTGTAATGGGAGTAGAA+AGG - Chr5:81000762-81000781 MsG0580028470.01.T03:CDS 45.0%
! GAAGAAGGTAGTGGTTGTTG+AGG + Chr5:81000235-81000254 None:intergenic 45.0%
!! TTGCCGCGTGTTTTTATAGC+TGG - Chr5:81000604-81000623 MsG0580028470.01.T03:CDS 45.0%
!!! GGTGGTGGAGTTTGTTTTTC+CGG + Chr5:81000361-81000380 None:intergenic 45.0%
!!! TAAATGGGTTTTGGAGGGGA+AGG + Chr5:81000382-81000401 None:intergenic 45.0%
AACCGGATTATGGGTCGAAC+CGG - Chr5:81000565-81000584 MsG0580028470.01.T03:CDS 50.0%
ATTGAATGCCACGTGTGTGC+TGG - Chr5:81000718-81000737 MsG0580028470.01.T03:CDS 50.0%
GACCGAACTGAACCGGATTA+TGG - Chr5:81000555-81000574 MsG0580028470.01.T03:CDS 50.0%
GCACACACGTGGCATTCAAT+TGG + Chr5:81000718-81000737 None:intergenic 50.0%
TTCGTCGAGATGAACGTGGA+AGG + Chr5:81000506-81000525 None:intergenic 50.0%
! GAAGGTAGTGGTTGTTGAGG+TGG + Chr5:81000232-81000251 None:intergenic 50.0%
! GTGGGTTGTGAAGAAGGTAG+TGG + Chr5:81000244-81000263 None:intergenic 50.0%
ACCTATGACCAGCACACACG+TGG + Chr5:81000729-81000748 None:intergenic 55.0%
ATACTTCACAAGCCTGCGCG+TGG - Chr5:81000432-81000451 MsG0580028470.01.T03:CDS 55.0%
ATGGGTCGAACCGGTCAAAC+CGG - Chr5:81000574-81000593 MsG0580028470.01.T03:CDS 55.0%
GAGTTTGAGGGGAGATGACC+AGG + Chr5:81000317-81000336 None:intergenic 55.0%
!! GACCGGTTCGACCCATAATC+CGG + Chr5:81000570-81000589 None:intergenic 55.0%
!!! ATGGGTTTTGGAGGGGAAGG+TGG + Chr5:81000379-81000398 None:intergenic 55.0%
ACACAATGACACACGCGCGC+CGG - Chr5:81000339-81000358 MsG0580028470.01.T03:CDS 60.0%
CGCGTTCGTCGAGATGAACG+TGG + Chr5:81000510-81000529 None:intergenic 60.0%
TGAGGGGAGATGACCAGGCA+CGG + Chr5:81000312-81000331 None:intergenic 60.0%
TGTTGGCGAGAAGGACGCGT+TGG + Chr5:81000282-81000301 None:intergenic 60.0%
! AGATTGGTCGAACCACGCGC+AGG + Chr5:81000447-81000466 None:intergenic 60.0%
! GATGAACGTGGAAGGCGCGT+AGG + Chr5:81000498-81000517 None:intergenic 60.0%
! GCCACGTGTGTGCTGGTCAT+AGG - Chr5:81000725-81000744 MsG0580028470.01.T03:CDS 60.0%
! GCGCGCGTGTGTCATTGTGT+GGG + Chr5:81000339-81000358 None:intergenic 60.0%
! GGAGGAGTGTGTTGGCGAGA+AGG + Chr5:81000291-81000310 None:intergenic 60.0%
!!! GGTTTTGGAGGGGAAGGTGG+TGG + Chr5:81000376-81000395 None:intergenic 60.0%
CCAACACACTCCTCCGTGCC+TGG - Chr5:81000296-81000315 MsG0580028470.01.T03:CDS 65.0%
CGACGAACGCGACGTGTCGA+TGG - Chr5:81000519-81000538 MsG0580028470.01.T03:CDS 65.0%
! CCAGGCACGGAGGAGTGTGT+TGG + Chr5:81000299-81000318 None:intergenic 65.0%
! GGCGCGCGTGTGTCATTGTG+TGG + Chr5:81000340-81000359 None:intergenic 65.0%
GGGGAGATGACCAGGCACGG+AGG + Chr5:81000309-81000328 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 81000121 81000888 81000121 ID=MsG0580028470.01;Name=MsG0580028470.01
Chr5 mRNA 81000121 81000888 81000121 ID=MsG0580028470.01.T01;Parent=MsG0580028470.01;Name=MsG0580028470.01.T01;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|0|0|2|0|238
Chr5 exon 81000121 81000647 81000121 ID=MsG0580028470.01.T01:exon:23805;Parent=MsG0580028470.01.T01
Chr5 exon 81000699 81000888 81000699 ID=MsG0580028470.01.T01:exon:23804;Parent=MsG0580028470.01.T01
Chr5 CDS 81000699 81000888 81000699 ID=MsG0580028470.01.T01:cds;Parent=MsG0580028470.01.T01
Chr5 CDS 81000121 81000647 81000121 ID=MsG0580028470.01.T01:cds;Parent=MsG0580028470.01.T01
Chr5 mRNA 81000121 81000888 81000121 ID=MsG0580028470.01.T02;Parent=MsG0580028470.01;Name=MsG0580028470.01.T02;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|0|0|2|0|233
Chr5 exon 81000121 81000601 81000121 ID=MsG0580028470.01.T02:exon:23807;Parent=MsG0580028470.01.T02
Chr5 exon 81000668 81000888 81000668 ID=MsG0580028470.01.T02:exon:23806;Parent=MsG0580028470.01.T02
Chr5 CDS 81000668 81000888 81000668 ID=MsG0580028470.01.T02:cds;Parent=MsG0580028470.01.T02
Chr5 CDS 81000121 81000601 81000121 ID=MsG0580028470.01.T02:cds;Parent=MsG0580028470.01.T02
Chr5 mRNA 81000121 81000888 81000121 ID=MsG0580028470.01.T03;Parent=MsG0580028470.01;Name=MsG0580028470.01.T03;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|255
Chr5 exon 81000121 81000888 81000121 ID=MsG0580028470.01.T03:exon:23808;Parent=MsG0580028470.01.T03
Chr5 CDS 81000121 81000888 81000121 ID=MsG0580028470.01.T03:cds;Parent=MsG0580028470.01.T03
Chr5 mRNA 81000121 81000471 81000121 ID=MsG0580028470.01.T04;Parent=MsG0580028470.01;Name=MsG0580028470.01.T04;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|116
Chr5 exon 81000121 81000471 81000121 ID=MsG0580028470.01.T04:exon:23809;Parent=MsG0580028470.01.T04
Chr5 CDS 81000121 81000471 81000121 ID=MsG0580028470.01.T04:cds;Parent=MsG0580028470.01.T04
Gene Sequence

>MsG0580028470.01.T03

ATGTGGAAACCGTGGGGAAAATCAACATCTAAGATTCATAACAAATCTTCTTCATCCTCATTCTCTTGTTCTTCCTTCAAAGACATTCAAACCCTTATTCTTCAAGAACCACCTCAACAACCACTACCTTCTTCACAACCCACAACAAAACAAGTCTTCCAACGCGTCCTTCTCGCCAACACACTCCTCCGTGCCTGGTCATCTCCCCTCAAACTCCCACACAATGACACACGCGCGCCGGAAAAACAAACTCCACCACCTTCCCCTCCAAAACCCATTTATTTTCGGGGAACAGAACAGCGCGTAGTGATATACTTCACAAGCCTGCGCGTGGTTCGACCAATCTTCGAAGACTGCAAAAGCGCACTCACAATCCTACGCGCCTTCCACGTTCATCTCGACGAACGCGACGTGTCGATGGATTCGTCTTTCTTGACCGAACTGAACCGGATTATGGGTCGAACCGGTCAAACCGGTTTATCATTGCCGCGTGTTTTTATAGCTGGAAAATATATCGGTGGAGGTGAAGAGATTAGATCAATGCACGAGATTGGAGAGCTTAAGAAAATGCTAGAAGATTTGCCTGTAGTGGATCCAATTGAATGCCACGTGTGTGCTGGTCATAGGTTTGTTCTATGTAACGTGTGTAATGGGAGTAGAAAGGTGTATAATGATAAAGCTGGATTTAAAGTTTGTAATGTTTGTAATGAGAATGGTCTTCTTAGGTGCCCTTCTTGTTTTCCTAATAACAATGAAATCAAAAATTGA

>MsG0580028470.01.T02

ATGTGGAAACCGTGGGGAAAATCAACATCTAAGATTCATAACAAATCTTCTTCATCCTCATTCTCTTGTTCTTCCTTCAAAGACATTCAAACCCTTATTCTTCAAGAACCACCTCAACAACCACTACCTTCTTCACAACCCACAACAAAACAAGTCTTCCAACGCGTCCTTCTCGCCAACACACTCCTCCGTGCCTGGTCATCTCCCCTCAAACTCCCACAGGGAACAGAACAGCGCGTAGTGATATACTTCACAAGCCTGCGCGTGGTTCGACCAATCTTCGAAGACTGCAAAAGCGCACTCACAATCCTACGCGCCTTCCACGTTCATCTCGACGAACGCGACGTGTCGATGGATTCGTCTTTCTTGACCGAACTGAACCGGATTATGGGTCGAACCGGTCAAACCGGTTTATCATTGCCGCGTGTTTTTATAGCTGGAAAATATATCGGTGGAGGTGAAGAGATTAGATCAATGCACGAGATTGGAGAGCTTAAGAAAATGCTAGAAGATTTGCCTGTAGTGGATCCAATTGAATGCCACGTGTGTGCTGGTCATAGGTTTGTTCTATGTAACGTGTGTAATGGGAGTAGAAAGGTGTATAATGATAAAGCTGGATTTAAAGTTTGTAATGTTTGTAATGAGAATGGTCTTCTTAGGTGCCCTTCTTGTTTTCCTAATAACAATGAAATCAAAAATTGA

>MsG0580028470.01.T01

ATGTGGAAACCGTGGGGAAAATCAACATCTAAGATTCATAACAAATCTTCTTCATCCTCATTCTCTTGTTCTTCCTTCAAAGACATTCAAACCCTTATTCTTCAAGAACCACCTCAACAACCACTACCTTCTTCACAACCCACAACAAAACAAGTCTTCCAACGCGTCCTTCTCGCCAACACACTCCTCCAAAAACAAACTCCACCACCTTCCCCTCCAAAACCCATTTATTTTCGGGGAACAGAACAGCGCGTAGTGATATACTTCACAAGCCTGCGCGTGGTTCGACCAATCTTCGAAGACTGCAAAAGCGCACTCACAATCCTACGCGCCTTCCACGTTCATCTCGACGAACGCGACGTGTCGATGGATTCGTCTTTCTTGACCGAACTGAACCGGATTATGGGTCGAACCGGTCAAACCGGTTTATCATTGCCGCGTGTTTTTATAGCTGGAAAATATATCGGTGGAGGTGAAGAGATTAGATCAATGCACGAGATTGGAGAGCTTAAGAAAATGCTAGAAGATTTGCCTGTAGTGGATCCAATTGAATGCCACGTGTGTGCTGGTCATAGGTTTGTTCTATGTAACGTGTGTAATGGGAGTAGAAAGGTGTATAATGATAAAGCTGGATTTAAAGTTTGTAATGTTTGTAATGAGAATGGTCTTCTTAGGTGCCCTTCTTGTTTTCCTAATAACAATGAAATCAAAAATTGA

>MsG0580028470.01.T04

ATGGATTCGTCTTTCTTGACCGAACTGAACCGGATTATGGGTCGAACCGGTCAAACCGGTTTATCATTGCCGCGTGTTTTTATAGCTGGAAAATATATCGGTGGAGGTGAAGAGATTAGATCAATGCACGAGATTGGAGAGCTTAAGAAAATGCTAGAAGATTTGCCTGTAGTGGATCCAATTGAATGCCACGTGTGTGCTGGTCATAGGTTTGTTCTATGTAACGTGTGTAATGGGAGTAGAAAGGTGTATAATGATAAAGCTGGATTTAAAGTTTGTAATGTTTGTAATGAGAATGGTCTTCTTAGGTGCCCTTCTTGTTTTCCTAATAACAATGAAATCAAAAATTGA
Protein sequence

>MsG0580028470.01.T03

MWKPWGKSTSKIHNKSSSSSFSCSSFKDIQTLILQEPPQQPLPSSQPTTKQVFQRVLLANTLLRAWSSPLKLPHNDTRAPEKQTPPPSPPKPIYFRGTEQRVVIYFTSLRVVRPIFEDCKSALTILRAFHVHLDERDVSMDSSFLTELNRIMGRTGQTGLSLPRVFIAGKYIGGGEEIRSMHEIGELKKMLEDLPVVDPIECHVCAGHRFVLCNVCNGSRKVYNDKAGFKVCNVCNENGLLRCPSCFPNNNEIKN*

>MsG0580028470.01.T02

MWKPWGKSTSKIHNKSSSSSFSCSSFKDIQTLILQEPPQQPLPSSQPTTKQVFQRVLLANTLLRAWSSPLKLPQGTEQRVVIYFTSLRVVRPIFEDCKSALTILRAFHVHLDERDVSMDSSFLTELNRIMGRTGQTGLSLPRVFIAGKYIGGGEEIRSMHEIGELKKMLEDLPVVDPIECHVCAGHRFVLCNVCNGSRKVYNDKAGFKVCNVCNENGLLRCPSCFPNNNEIKN*

>MsG0580028470.01.T01

MWKPWGKSTSKIHNKSSSSSFSCSSFKDIQTLILQEPPQQPLPSSQPTTKQVFQRVLLANTLLQKQTPPPSPPKPIYFRGTEQRVVIYFTSLRVVRPIFEDCKSALTILRAFHVHLDERDVSMDSSFLTELNRIMGRTGQTGLSLPRVFIAGKYIGGGEEIRSMHEIGELKKMLEDLPVVDPIECHVCAGHRFVLCNVCNGSRKVYNDKAGFKVCNVCNENGLLRCPSCFPNNNEIKN*

>MsG0580028470.01.T04

MDSSFLTELNRIMGRTGQTGLSLPRVFIAGKYIGGGEEIRSMHEIGELKKMLEDLPVVDPIECHVCAGHRFVLCNVCNGSRKVYNDKAGFKVCNVCNENGLLRCPSCFPNNNEIKN*