Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039861.01.T01 | XP_003624221.2 | 98.919 | 185 | 2 | 0 | 1 | 185 | 1 | 185 | 1.53E-131 | 376 |
| MsG0780039861.01.T02 | XP_003624221.2 | 98.895 | 181 | 2 | 0 | 1 | 181 | 5 | 185 | 1.12E-128 | 369 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047815.01 | MsG0780039861.01 | 0.861317 | 1.116674e-63 | 3.146980e-60 |
| MsG0080048258.01 | MsG0780039861.01 | 0.875943 | 2.068010e-68 | 9.843374e-65 |
| MsG0080048311.01 | MsG0780039861.01 | 0.859750 | 3.332486e-63 | 8.896306e-60 |
| MsG0080048701.01 | MsG0780039861.01 | 0.818800 | 1.622718e-52 | 1.226821e-49 |
| MsG0080048775.01 | MsG0780039861.01 | 0.866200 | 3.392587e-65 | 1.133754e-61 |
| MsG0080048781.01 | MsG0780039861.01 | 0.914854 | 1.175546e-84 | 2.984757e-80 |
| MsG0080048996.01 | MsG0780039861.01 | 0.815033 | 1.137154e-51 | 7.753852e-49 |
| MsG0080049140.01 | MsG0780039861.01 | 0.878452 | 2.777321e-69 | 1.451134e-65 |
| MsG0180000076.01 | MsG0780039861.01 | 0.809585 | 1.759774e-50 | 1.037303e-47 |
| MsG0180000111.01 | MsG0780039861.01 | 0.886096 | 4.602367e-72 | 3.241524e-68 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039861.01.T01 | MTR_7g080550 | 98.919 | 185 | 2 | 0 | 1 | 185 | 1 | 185 | 1.86e-135 | 376 |
| MsG0780039861.01.T01 | MTR_8g081530 | 69.714 | 175 | 46 | 2 | 14 | 185 | 4 | 174 | 5.22e-74 | 220 |
| MsG0780039861.01.T02 | MTR_7g080550 | 98.895 | 181 | 2 | 0 | 1 | 181 | 5 | 185 | 1.36e-132 | 369 |
| MsG0780039861.01.T02 | MTR_8g081530 | 69.714 | 175 | 46 | 2 | 10 | 181 | 4 | 174 | 5.34e-74 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039861.01.T01 | AT5G16250 | 68.449 | 187 | 53 | 3 | 1 | 185 | 1 | 183 | 4.16e-81 | 239 |
| MsG0780039861.01.T01 | AT3G02640 | 67.380 | 187 | 57 | 3 | 1 | 185 | 1 | 185 | 5.90e-78 | 231 |
| MsG0780039861.01.T01 | AT5G36800 | 61.017 | 177 | 56 | 4 | 18 | 185 | 44 | 216 | 2.86e-67 | 205 |
| MsG0780039861.01.T01 | AT5G36710 | 61.017 | 177 | 56 | 4 | 18 | 185 | 44 | 216 | 2.86e-67 | 205 |
| MsG0780039861.01.T01 | AT5G36800 | 61.017 | 177 | 56 | 4 | 18 | 185 | 9 | 181 | 4.21e-67 | 203 |
| MsG0780039861.01.T01 | AT5G36710 | 61.017 | 177 | 56 | 4 | 18 | 185 | 9 | 181 | 4.21e-67 | 203 |
| MsG0780039861.01.T02 | AT5G16250 | 69.061 | 181 | 52 | 2 | 3 | 181 | 5 | 183 | 1.48e-80 | 237 |
| MsG0780039861.01.T02 | AT3G02640 | 67.956 | 181 | 55 | 2 | 3 | 181 | 6 | 185 | 1.40e-77 | 229 |
| MsG0780039861.01.T02 | AT5G36800 | 61.017 | 177 | 56 | 4 | 14 | 181 | 44 | 216 | 2.60e-67 | 205 |
| MsG0780039861.01.T02 | AT5G36710 | 61.017 | 177 | 56 | 4 | 14 | 181 | 44 | 216 | 2.60e-67 | 205 |
Find 43 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGTAGCAACCATATGAG+CGG | 0.648596 | 7:+69821044 | None:intergenic |
| AACGGCTATATTTCAAGGGT+CGG | 0.489003 | 7:-69821019 | MsG0780039861.01.T01:CDS |
| AAGATGAAGATTTGAAGGAT+TGG | 0.477591 | 7:-69820768 | MsG0780039861.01.T01:CDS |
| AAGATTTGAAGGATTGGCCA+TGG | 0.611621 | 7:-69820762 | MsG0780039861.01.T01:CDS |
| AAGTATTATGCTTGTGTTGA+AGG | 0.407930 | 7:-69820838 | MsG0780039861.01.T01:CDS |
| ACCATTGCTGGAGCTGTCTC+AGG | 0.396308 | 7:-69821099 | MsG0780039861.01.T01:CDS |
| AGAGGACATAGTTGCAGAAA+AGG | 0.386856 | 7:+69821214 | None:intergenic |
| AGCTTGAAGCTGCACCTAAG+AGG | 0.489271 | 7:+69821196 | None:intergenic |
| AGGAAGAAGCCGCAGACGGA+AGG | 0.533462 | 7:+69821141 | None:intergenic |
| ATTATGCTTGTGTTGAAGGT+GGG | 0.566434 | 7:-69820834 | MsG0780039861.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGATGAAGATTTGAAGGAT+TGG | - | Chr7:69821261-69821280 | MsG0780039861.01.T01:CDS | 30.0% | |
| AAGTATTATGCTTGTGTTGA+AGG | - | Chr7:69821191-69821210 | MsG0780039861.01.T01:CDS | 30.0% | |
| TCAACAAGATGAAGATTTGA+AGG | - | Chr7:69821256-69821275 | MsG0780039861.01.T01:CDS | 30.0% | |
| TTCTAACGGCTATATTTCAA+GGG | - | Chr7:69821006-69821025 | MsG0780039861.01.T01:CDS | 30.0% | |
| ATTATGCTTGTGTTGAAGGT+GGG | - | Chr7:69821195-69821214 | MsG0780039861.01.T01:CDS | 35.0% | |
| GTTCTAACGGCTATATTTCA+AGG | - | Chr7:69821005-69821024 | MsG0780039861.01.T01:CDS | 35.0% | |
| TATGGTTGCTACTGTTCTAA+CGG | - | Chr7:69820992-69821011 | MsG0780039861.01.T01:CDS | 35.0% | |
| TATTATGCTTGTGTTGAAGG+TGG | - | Chr7:69821194-69821213 | MsG0780039861.01.T01:CDS | 35.0% | |
| AACAGTAGCAACCATATGAG+CGG | + | Chr7:69820988-69821007 | None:intergenic | 40.0% | |
| AACGGCTATATTTCAAGGGT+CGG | - | Chr7:69821010-69821029 | MsG0780039861.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 69820747 | 69821304 | 69820747 | ID=MsG0780039861.01;Name=MsG0780039861.01 |
| Chr7 | mRNA | 69820747 | 69821304 | 69820747 | ID=MsG0780039861.01.T01;Parent=MsG0780039861.01;Name=MsG0780039861.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|185 |
| Chr7 | exon | 69820747 | 69821304 | 69820747 | ID=MsG0780039861.01.T01:exon:12852;Parent=MsG0780039861.01.T01 |
| Chr7 | CDS | 69820747 | 69821304 | 69820747 | ID=MsG0780039861.01.T01:cds;Parent=MsG0780039861.01.T01 |
| Chr7 | mRNA | 69820747 | 69821292 | 69820747 | ID=MsG0780039861.01.T02;Parent=MsG0780039861.01;Name=MsG0780039861.01.T02;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|0|1|0|181 |
| Chr7 | exon | 69820747 | 69821292 | 69820747 | ID=MsG0780039861.01.T02:exon:12853;Parent=MsG0780039861.01.T02 |
| Chr7 | CDS | 69820747 | 69821292 | 69820747 | ID=MsG0780039861.01.T02:cds;Parent=MsG0780039861.01.T02 |
| Gene Sequence |
>MsG0780039861.01.T01 ATGGGTCTCTCAATGAACAATCCTCCAGCTACAATCACACCTCGTCACTACAACATCCACAAACTCTTCCTTTTCTGCAACTATGTCCTCTTAGGTGCAGCTTCAAGCTGTATCTTCCTCACTCTTTCGCTCCGCTTAGTCCCTTCCGTCTGCGGCTTCTTCCTTATCCTTCTCCATGTCTTCACCATTGCTGGAGCTGTCTCAGGCTGTGCTGCTGTTGGTGCTAACCGTTGGTATTCCGCTCATATGGTTGCTACTGTTCTAACGGCTATATTTCAAGGGTCGGTTTCTGTTCTTGTTTTTACGAGGACGAGTGATTTTCTTGGTGAGTTGCAGTCTTATGTAAGGGAAGAAGATGCTTCTGTGATTTTGAAACTTTCTGGTGGACTTGCTATTTTGATCTTTTGCCTTGAGTGGGTTGTTTTGACTCTGGCGTTTTTCTTGAAGTATTATGCTTGTGTTGAAGGTGGGAATACTTGTAGGACTGTTGTTTTGGGAAGTGCTAAGGTTCAACAAGATGAAGATTTGAAGGATTGGCCATGGCCTTTCCAGGTTTGA >MsG0780039861.01.T02 ATGAACAATCCTCCAGCTACAATCACACCTCGTCACTACAACATCCACAAACTCTTCCTTTTCTGCAACTATGTCCTCTTAGGTGCAGCTTCAAGCTGTATCTTCCTCACTCTTTCGCTCCGCTTAGTCCCTTCCGTCTGCGGCTTCTTCCTTATCCTTCTCCATGTCTTCACCATTGCTGGAGCTGTCTCAGGCTGTGCTGCTGTTGGTGCTAACCGTTGGTATTCCGCTCATATGGTTGCTACTGTTCTAACGGCTATATTTCAAGGGTCGGTTTCTGTTCTTGTTTTTACGAGGACGAGTGATTTTCTTGGTGAGTTGCAGTCTTATGTAAGGGAAGAAGATGCTTCTGTGATTTTGAAACTTTCTGGTGGACTTGCTATTTTGATCTTTTGCCTTGAGTGGGTTGTTTTGACTCTGGCGTTTTTCTTGAAGTATTATGCTTGTGTTGAAGGTGGGAATACTTGTAGGACTGTTGTTTTGGGAAGTGCTAAGGTTCAACAAGATGAAGATTTGAAGGATTGGCCATGGCCTTTCCAGGTTTGA |
| Protein sequence |
>MsG0780039861.01.T01 MGLSMNNPPATITPRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHVFTIAGAVSGCAAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRTSDFLGELQSYVREEDASVILKLSGGLAILIFCLEWVVLTLAFFLKYYACVEGGNTCRTVVLGSAKVQQDEDLKDWPWPFQV* >MsG0780039861.01.T02 MNNPPATITPRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHVFTIAGAVSGCAAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRTSDFLGELQSYVREEDASVILKLSGGLAILIFCLEWVVLTLAFFLKYYACVEGGNTCRTVVLGSAKVQQDEDLKDWPWPFQV* |
