AlfalfaGEDB | MsG0580028553.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028553.01

KEGG: K13424
GO: GO:0003700 GO:0006355 GO:0043565
PFAM: PF03106

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028553.01.T02	AES98893.1	91.159	328	28	1	1	328	1	327	0	611
MsG0580028553.01.T01	RHN56682.1	88.398	181	20	1	54	234	32	211	4.25E-113	333

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028553.01.T01	Q6IEQ7	48.696	115	53	3	103	217	197	305	1.91E-26	110
MsG0580028553.01.T02	Q6B6R4	51.402	107	46	3	205	311	205	305	4.29E-26	111

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028553.01.T01	A0A396HTL3	88.398	181	20	1	54	234	32	211	2.03e-113	333
MsG0580028553.01.T02	G7IXC7	91.159	328	28	1	1	328	1	327	0.0	611

TFs/TRs:

Gene ID	Type	Classification
MsG0580028553.01.T01	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380014517.01	MsG0580028553.01	0.819635	1.047377e-52	8.104133e-50
MsG0280010253.01	MsG0580028553.01	0.807310	5.381152e-50	2.987340e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028553.01.T02	MTR_5g074200	91.159	328	28	1	1	328	1	327	0.0	611
MsG0580028553.01.T02	MTR_5g093540	65.244	328	35	6	1	328	1	249	4.69e-135	384
MsG0580028553.01.T02	MTR_5g093530	51.042	192	92	1	1	190	5	196	1.29e-51	179
MsG0580028553.01.T02	MTR_5g074130	51.053	190	93	0	1	190	5	194	5.52e-51	180
MsG0580028553.01.T02	MTR_5g073850	63.433	134	47	2	102	234	34	166	5.84e-50	164
MsG0580028553.01.T02	MTR_5g073460	40.385	260	144	4	9	261	12	267	1.21e-49	176
MsG0580028553.01.T02	MTR_5g074450	48.744	199	92	2	4	197	7	200	4.59e-49	172
MsG0580028553.01.T02	MTR_5g073840	47.938	194	96	3	1	190	4	196	7.17e-49	164
MsG0580028553.01.T02	MTR_3g464010	51.479	169	78	1	4	168	7	175	4.95e-47	169
MsG0580028553.01.T02	MTR_5g073920	44.196	224	112	3	4	220	7	224	5.56e-47	169
MsG0580028553.01.T02	MTR_5g074090	46.667	195	96	5	4	195	7	196	2.46e-46	157
MsG0580028553.01.T02	MTR_5g073480	38.346	266	142	4	9	261	12	268	5.30e-46	165
MsG0580028553.01.T02	MTR_5g073620	55.195	154	69	0	15	168	18	171	1.47e-44	163
MsG0580028553.01.T02	MTR_5g074190	50.000	168	81	1	1	168	1	165	3.19e-44	158
MsG0580028553.01.T02	MTR_5g074150	42.793	222	121	4	15	234	29	246	5.06e-44	152
MsG0580028553.01.T02	MTR_5g073960	41.071	224	121	3	4	216	7	230	3.98e-43	154
MsG0580028553.01.T02	MTR_5g073880	50.360	139	69	0	1	139	5	143	9.06e-41	144
MsG0580028553.01.T02	MTR_5g073810	42.381	210	111	4	9	213	12	216	5.22e-39	143
MsG0580028553.01.T02	MTR_6g078770	39.409	203	117	3	15	215	109	307	2.88e-37	137
MsG0580028553.01.T02	MTR_5g073610	42.703	185	101	3	15	194	18	202	1.48e-36	134
MsG0580028553.01.T02	MTR_6g078610	39.796	196	112	3	15	208	594	785	2.79e-33	130
MsG0580028553.01.T02	MTR_5g073750	42.857	161	91	1	4	163	7	167	4.73e-33	124
MsG0580028553.01.T02	MTR_6g075870	36.932	176	109	1	12	185	610	785	9.15e-32	125
MsG0580028553.01.T02	MTR_5g073860	37.668	223	125	5	1	216	18	233	1.22e-31	122
MsG0580028553.01.T02	MTR_5g074400	49.655	145	60	5	192	328	213	352	3.82e-31	123
MsG0580028553.01.T02	MTR_5g074400	58.065	62	25	1	220	280	420	481	1.13e-15	78.2
MsG0580028553.01.T02	MTR_5g073470	41.916	167	66	3	9	168	5	147	8.79e-27	107
MsG0580028553.01.T02	MTR_3g031220	46.923	130	62	3	200	328	178	301	3.74e-25	104
MsG0580028553.01.T02	MTR_3g031220	48.462	130	60	3	200	328	178	301	6.09e-25	105
MsG0580028553.01.T02	MTR_3g031220	48.718	78	36	2	220	296	365	439	4.37e-14	73.2
MsG0580028553.01.T02	MTR_5g074070	41.026	156	70	3	1	155	4	138	1.01e-24	100
MsG0580028553.01.T02	MTR_7g071120	54.651	86	35	2	202	286	150	232	3.50e-23	100
MsG0580028553.01.T02	MTR_7g071120	56.667	60	25	1	220	278	324	383	3.02e-14	73.6
MsG0580028553.01.T02	MTR_7g100510	64.706	68	23	1	221	288	190	256	7.00e-23	99.0
MsG0580028553.01.T02	MTR_7g100510	47.368	76	38	2	221	294	362	437	2.03e-12	68.2
MsG0580028553.01.T02	MTR_6g078620	35.593	177	83	3	3	178	80	226	3.03e-22	93.6
MsG0580028553.01.T02	MTR_4g013260	46.602	103	51	3	215	316	179	278	1.40e-21	95.5
MsG0580028553.01.T02	MTR_4g013260	55.000	60	26	1	220	278	336	395	3.63e-13	70.5
MsG0580028553.01.T02	MTR_3g056100	59.375	64	26	0	220	283	243	306	4.55e-21	94.4
MsG0580028553.01.T02	MTR_3g056100	52.941	68	29	2	220	286	402	467	1.26e-14	75.1
MsG0580028553.01.T02	MTR_5g094430	43.689	103	48	2	181	283	171	263	4.79e-21	94.0
MsG0580028553.01.T02	MTR_5g094430	55.000	60	26	1	220	278	364	423	3.49e-14	73.6
MsG0580028553.01.T02	MTR_3g056100	59.375	64	26	0	220	283	243	306	5.19e-21	94.0
MsG0580028553.01.T02	MTR_3g056100	52.941	68	29	2	220	286	415	480	1.41e-14	74.7
MsG0580028553.01.T02	MTR_3g056100	59.375	64	26	0	220	283	99	162	6.22e-21	93.2
MsG0580028553.01.T02	MTR_3g056100	52.941	68	29	2	220	286	258	323	2.12e-14	73.9
MsG0580028553.01.T02	MTR_5g094430	46.739	92	40	1	192	283	47	129	7.71e-21	92.8
MsG0580028553.01.T02	MTR_5g094430	55.000	60	26	1	220	278	230	289	5.06e-14	72.8
MsG0580028553.01.T02	MTR_3g056100	59.375	64	26	0	220	283	99	162	7.94e-21	93.2
MsG0580028553.01.T02	MTR_3g056100	52.941	68	29	2	220	286	271	336	2.64e-14	73.6
MsG0580028553.01.T02	MTR_5g094430	46.739	92	40	1	192	283	69	151	8.20e-21	92.8
MsG0580028553.01.T02	MTR_5g094430	55.000	60	26	1	220	278	252	311	5.26e-14	72.8
MsG0580028553.01.T02	MTR_5g074080	63.380	71	25	1	117	186	1	71	1.82e-20	86.7
MsG0580028553.01.T02	MTR_6g078770	31.034	203	103	4	15	215	109	276	5.48e-20	89.4
MsG0580028553.01.T02	MTR_7g011820	52.874	87	39	1	203	287	211	297	6.74e-20	90.5
MsG0580028553.01.T02	MTR_7g011820	50.000	76	34	2	220	294	410	482	7.59e-15	75.5
MsG0580028553.01.T02	MTR_7g080020	42.623	122	49	3	183	283	222	343	8.88e-20	90.5
MsG0580028553.01.T02	MTR_7g080020	53.226	62	28	1	220	280	493	554	1.00e-13	72.4
MsG0580028553.01.T02	MTR_5g042920	64.407	59	21	0	220	278	151	209	9.45e-20	89.7
MsG0580028553.01.T02	MTR_5g042920	56.667	60	25	1	220	278	320	379	5.90e-15	75.5
MsG0580028553.01.T02	MTR_3g009470	41.803	122	57	4	167	283	231	343	8.33e-19	87.8
MsG0580028553.01.T02	MTR_3g009470	54.839	62	27	1	220	280	495	556	1.41e-13	72.0
MsG0580028553.01.T02	MTR_3g009470	41.803	122	57	4	167	283	231	343	1.36e-18	86.7
MsG0580028553.01.T02	MTR_7g080000	50.588	85	40	1	221	303	235	319	1.64e-17	84.0
MsG0580028553.01.T02	MTR_7g080000	53.226	62	28	1	220	280	432	493	1.00e-13	72.4
MsG0580028553.01.T02	MTR_4g082580	52.857	70	32	1	220	288	145	214	2.25e-16	79.7
MsG0580028553.01.T02	MTR_4g082580	47.059	85	43	2	220	302	278	362	2.37e-14	73.6
MsG0580028553.01.T02	MTR_1g077780	50.704	71	34	1	220	289	62	132	2.56e-16	75.1
MsG0580028553.01.T02	MTR_5g073500	33.784	148	67	1	10	157	1	117	7.08e-15	70.9
MsG0580028553.01.T02	MTR_5g097490	28.977	176	92	3	150	304	1	164	2.79e-14	73.6
MsG0580028553.01.T02	MTR_5g097490	46.667	60	31	1	220	278	252	311	1.45e-11	65.1
MsG0580028553.01.T02	MTR_6g046600	62.264	53	19	1	236	288	18	69	3.01e-14	68.6
MsG0580028553.01.T02	MTR_5g073660	34.839	155	57	3	15	166	37	150	4.03e-14	72.0
MsG0580028553.01.T02	MTR_3g106060	38.889	90	49	3	221	304	139	228	1.19e-13	70.9
MsG0580028553.01.T02	MTR_5g091390	50.000	68	32	2	220	286	157	223	4.03e-13	69.3
MsG0580028553.01.T02	MTR_1g086790	50.000	68	31	2	220	286	158	223	8.26e-13	68.6
MsG0580028553.01.T02	MTR_3g095080	54.386	57	25	1	220	275	168	224	9.47e-13	67.4
MsG0580028553.01.T02	MTR_8g092140	54.386	57	25	1	220	275	160	216	1.28e-12	67.0
MsG0580028553.01.T02	MTR_8g096730	47.619	63	32	1	220	281	70	132	1.30e-12	65.1
MsG0580028553.01.T02	MTR_3g095080	54.386	57	25	1	220	275	144	200	1.32e-12	66.6
MsG0580028553.01.T02	MTR_4g023690	45.205	73	39	1	68	140	17	88	1.35e-12	63.2
MsG0580028553.01.T02	MTR_8g007270	57.895	57	23	1	220	275	154	210	1.46e-12	66.6
MsG0580028553.01.T02	MTR_5g073650	40.870	115	59	3	103	213	5	114	1.65e-12	67.8
MsG0580028553.01.T02	MTR_2g033820	50.794	63	30	1	220	281	203	265	1.98e-12	67.8
MsG0580028553.01.T02	MTR_4g094412	49.180	61	29	2	220	278	234	294	2.63e-12	67.8
MsG0580028553.01.T02	MTR_4g107970	50.820	61	28	2	220	278	178	238	2.93e-12	67.8
MsG0580028553.01.T02	MTR_4g094412	49.180	61	29	2	220	278	243	303	2.98e-12	67.8
MsG0580028553.01.T02	MTR_7g109800	50.000	78	35	3	220	296	145	219	3.10e-12	66.6
MsG0580028553.01.T02	MTR_4g094588	45.455	77	40	2	220	295	178	253	3.42e-12	67.0
MsG0580028553.01.T02	MTR_2g105060	38.095	84	50	2	221	302	95	178	3.52e-12	65.9
MsG0580028553.01.T02	MTR_7g028415	49.206	63	31	1	220	281	93	155	4.24e-12	63.9
MsG0580028553.01.T02	MTR_7g028710	49.206	63	31	1	220	281	93	155	4.24e-12	63.9
MsG0580028553.01.T02	MTR_4g107970	50.820	61	28	2	220	278	255	315	4.51e-12	67.4
MsG0580028553.01.T02	MTR_2g105060	38.095	84	50	2	221	302	170	253	4.85e-12	66.2
MsG0580028553.01.T02	MTR_7g079010	54.386	57	25	1	220	275	141	197	5.38e-12	64.7
MsG0580028553.01.T02	MTR_4g122530	53.333	60	27	1	220	278	208	267	6.49e-12	66.2
MsG0580028553.01.T02	MTR_5g097500	42.105	76	44	0	207	282	70	145	6.55e-12	65.9
MsG0580028553.01.T02	MTR_8g092010	53.333	60	27	1	220	278	108	167	8.03e-12	63.5
MsG0580028553.01.T02	MTR_5g073770	43.137	102	38	1	67	168	31	112	1.32e-11	65.5
MsG0580028553.01.T02	MTR_3g104750	52.632	57	26	1	220	275	104	160	1.36e-11	62.4
MsG0580028553.01.T02	MTR_4g132430	43.939	66	35	2	220	283	127	192	1.40e-11	65.1
MsG0580028553.01.T02	MTR_4g132430	44.615	65	34	2	220	282	261	325	5.14e-11	63.9
MsG0580028553.01.T02	MTR_7g062220	50.000	58	28	1	220	276	124	181	8.04e-11	62.4
MsG0580028553.01.T01	MTR_5g074200	88.398	181	20	1	54	234	148	327	4.76e-116	335
MsG0580028553.01.T01	MTR_5g074200	64.286	84	28	1	1	84	1	82	1.61e-25	102
MsG0580028553.01.T01	MTR_5g093540	55.839	274	56	6	1	234	1	249	2.92e-87	259
MsG0580028553.01.T01	MTR_5g073850	53.659	164	39	4	13	140	4	166	3.12e-46	151
MsG0580028553.01.T01	MTR_5g074400	49.655	145	60	5	98	234	213	352	1.38e-31	121
MsG0580028553.01.T01	MTR_5g074400	58.065	62	25	1	126	186	420	481	5.43e-16	77.0
MsG0580028553.01.T01	MTR_3g031220	46.923	130	62	3	106	234	178	301	1.33e-25	103
MsG0580028553.01.T01	MTR_3g031220	48.462	130	60	3	106	234	178	301	2.66e-25	103
MsG0580028553.01.T01	MTR_3g031220	48.718	78	36	2	126	202	365	439	2.84e-14	72.0
MsG0580028553.01.T01	MTR_7g071120	54.651	86	35	2	108	192	150	232	2.38e-23	98.2
MsG0580028553.01.T01	MTR_7g071120	56.667	60	25	1	126	184	324	383	1.19e-14	72.8
MsG0580028553.01.T01	MTR_7g100510	64.706	68	23	1	127	194	190	256	5.93e-23	96.7
MsG0580028553.01.T01	MTR_7g100510	47.368	76	38	2	127	200	362	437	7.72e-13	67.4
MsG0580028553.01.T01	MTR_4g013260	46.602	103	51	3	121	222	179	278	4.77e-22	94.4
MsG0580028553.01.T01	MTR_4g013260	55.000	60	26	1	126	184	336	395	2.13e-13	69.3
MsG0580028553.01.T01	MTR_3g056100	59.375	64	26	0	126	189	243	306	1.65e-21	93.2
MsG0580028553.01.T01	MTR_3g056100	52.941	68	29	2	126	192	402	467	1.19e-14	73.2
MsG0580028553.01.T01	MTR_3g056100	59.375	64	26	0	126	189	243	306	1.72e-21	92.8
MsG0580028553.01.T01	MTR_3g056100	52.941	68	29	2	126	192	415	480	1.24e-14	72.8
MsG0580028553.01.T01	MTR_3g056100	59.375	64	26	0	126	189	99	162	2.24e-21	92.0
MsG0580028553.01.T01	MTR_3g056100	52.941	68	29	2	126	192	258	323	1.83e-14	72.4
MsG0580028553.01.T01	MTR_3g056100	59.375	64	26	0	126	189	99	162	2.59e-21	92.0
MsG0580028553.01.T01	MTR_3g056100	52.941	68	29	2	126	192	271	336	2.11e-14	72.0
MsG0580028553.01.T01	MTR_5g094430	42.593	108	52	2	87	194	171	268	3.21e-21	92.0
MsG0580028553.01.T01	MTR_5g094430	53.226	62	28	1	126	186	364	425	2.38e-14	72.0
MsG0580028553.01.T01	MTR_5g094430	45.361	97	44	1	98	194	47	134	4.19e-21	91.3
MsG0580028553.01.T01	MTR_5g094430	53.226	62	28	1	126	186	230	291	3.19e-14	71.6
MsG0580028553.01.T01	MTR_5g094430	45.361	97	44	1	98	194	69	156	4.29e-21	91.3
MsG0580028553.01.T01	MTR_5g094430	53.226	62	28	1	126	186	252	313	3.26e-14	71.6
MsG0580028553.01.T01	MTR_5g042920	64.407	59	21	0	126	184	151	209	2.62e-20	89.0
MsG0580028553.01.T01	MTR_5g042920	54.839	62	27	1	126	186	320	381	4.15e-15	74.3
MsG0580028553.01.T01	MTR_7g011820	52.874	87	39	1	109	193	211	297	4.93e-20	88.6
MsG0580028553.01.T01	MTR_7g011820	44.898	98	44	4	126	217	410	503	4.52e-15	74.3
MsG0580028553.01.T01	MTR_7g080020	41.600	125	52	3	86	189	219	343	6.78e-20	88.6
MsG0580028553.01.T01	MTR_7g080020	53.226	62	28	1	126	186	493	554	5.06e-14	71.2
MsG0580028553.01.T01	MTR_3g009470	41.803	122	57	4	73	189	231	343	4.68e-19	85.9
MsG0580028553.01.T01	MTR_3g009470	54.839	62	27	1	126	186	495	556	6.18e-14	70.9
MsG0580028553.01.T01	MTR_3g009470	41.803	122	57	4	73	189	231	343	6.22e-19	85.5
MsG0580028553.01.T01	MTR_7g080000	57.143	63	27	0	127	189	235	297	2.01e-17	81.3
MsG0580028553.01.T01	MTR_7g080000	53.226	62	28	1	126	186	432	493	5.22e-14	71.2
MsG0580028553.01.T01	MTR_4g082580	52.857	70	32	1	126	194	145	214	8.50e-17	79.0
MsG0580028553.01.T01	MTR_4g082580	47.059	85	43	2	126	208	278	362	1.49e-14	72.4
MsG0580028553.01.T01	MTR_1g077780	50.704	71	34	1	126	195	62	132	1.06e-16	74.7
MsG0580028553.01.T01	MTR_5g097490	30.114	176	90	3	56	210	1	164	7.26e-15	73.2
MsG0580028553.01.T01	MTR_5g097490	46.667	60	31	1	126	184	252	311	1.08e-11	63.9
MsG0580028553.01.T01	MTR_6g046600	62.264	53	19	1	142	194	18	69	3.47e-14	67.4
MsG0580028553.01.T01	MTR_3g106060	38.889	90	49	3	127	210	139	228	8.00e-14	69.7
MsG0580028553.01.T01	MTR_5g091390	50.000	68	32	2	126	192	157	223	2.75e-13	68.2
MsG0580028553.01.T01	MTR_1g086790	50.000	68	31	2	126	192	158	223	4.85e-13	67.8
MsG0580028553.01.T01	MTR_3g095080	54.386	57	25	1	126	181	168	224	6.74e-13	66.2
MsG0580028553.01.T01	MTR_8g096730	47.619	63	32	1	126	187	70	132	7.24e-13	64.3
MsG0580028553.01.T01	MTR_3g095080	54.386	57	25	1	126	181	144	200	8.83e-13	65.9
MsG0580028553.01.T01	MTR_8g092140	54.386	57	25	1	126	181	160	216	9.24e-13	65.9
MsG0580028553.01.T01	MTR_8g007270	57.895	57	23	1	126	181	154	210	9.52e-13	65.9
MsG0580028553.01.T01	MTR_4g107970	50.820	61	28	2	126	184	178	238	9.57e-13	67.4
MsG0580028553.01.T01	MTR_2g033820	50.794	63	30	1	126	187	203	265	1.02e-12	67.0
MsG0580028553.01.T01	MTR_4g094412	49.180	61	29	2	126	184	234	294	1.09e-12	67.0
MsG0580028553.01.T01	MTR_4g107970	50.820	61	28	2	126	184	255	315	1.15e-12	67.0
MsG0580028553.01.T01	MTR_7g109800	50.000	78	35	3	126	202	145	219	1.15e-12	66.2
MsG0580028553.01.T01	MTR_4g094412	49.180	61	29	2	126	184	243	303	1.19e-12	67.0
MsG0580028553.01.T01	MTR_4g094588	45.455	77	40	2	126	201	178	253	1.58e-12	66.2
MsG0580028553.01.T01	MTR_4g122530	53.333	60	27	1	126	184	208	267	2.89e-12	65.9
MsG0580028553.01.T01	MTR_2g105060	44.776	67	35	2	127	191	95	161	3.50e-12	64.3
MsG0580028553.01.T01	MTR_7g028415	49.206	63	31	1	126	187	93	155	3.66e-12	63.2
MsG0580028553.01.T01	MTR_7g028710	49.206	63	31	1	126	187	93	155	3.66e-12	63.2
MsG0580028553.01.T01	MTR_5g073860	50.000	60	30	0	1	60	18	77	3.86e-12	65.5
MsG0580028553.01.T01	MTR_5g097500	42.105	76	44	0	113	188	70	145	4.35e-12	64.7
MsG0580028553.01.T01	MTR_5g097500	40.580	69	40	1	126	193	230	298	8.31e-11	61.2
MsG0580028553.01.T01	MTR_2g105060	44.776	67	35	2	127	191	170	236	4.47e-12	64.7
MsG0580028553.01.T01	MTR_7g079010	54.386	57	25	1	126	181	141	197	4.57e-12	63.5
MsG0580028553.01.T01	MTR_5g073460	50.000	54	27	0	9	62	12	65	4.69e-12	65.5
MsG0580028553.01.T01	MTR_4g132430	43.939	66	35	2	126	189	127	192	5.11e-12	64.7
MsG0580028553.01.T01	MTR_5g074130	47.541	61	32	0	1	61	5	65	5.15e-12	65.5
MsG0580028553.01.T01	MTR_5g093530	45.902	61	33	0	1	61	5	65	5.47e-12	65.1
MsG0580028553.01.T01	MTR_5g073920	45.902	61	33	0	4	64	7	67	5.96e-12	65.1
MsG0580028553.01.T01	MTR_8g092010	53.333	60	27	1	126	184	108	167	6.03e-12	62.8
MsG0580028553.01.T01	MTR_3g464010	48.276	58	30	0	4	61	7	64	7.58e-12	64.7
MsG0580028553.01.T01	MTR_5g073480	50.000	54	27	0	9	62	12	65	7.74e-12	64.7
MsG0580028553.01.T01	MTR_3g053330	37.209	86	52	2	127	210	252	337	8.65e-12	64.3
MsG0580028553.01.T01	MTR_3g104750	52.632	57	26	1	126	181	104	160	8.86e-12	62.0
MsG0580028553.01.T01	MTR_4g132430	44.615	65	34	2	126	188	261	325	1.55e-11	63.9
MsG0580028553.01.T01	MTR_5g074450	46.552	58	31	0	4	61	7	64	2.29e-11	63.2
MsG0580028553.01.T01	MTR_2g023930	48.333	60	29	2	127	184	306	365	2.51e-11	63.2
MsG0580028553.01.T01	MTR_7g062220	50.000	58	28	1	126	182	124	181	4.64e-11	62.0
MsG0580028553.01.T01	MTR_5g073960	45.283	53	29	0	9	61	12	64	7.41e-11	61.6
MsG0580028553.01.T01	MTR_1g015140	37.255	102	58	3	81	181	62	158	8.29e-11	59.3
MsG0580028553.01.T01	MTR_5g073880	41.176	68	37	1	1	68	5	69	8.68e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028553.01.T02	AT1G13960	60.606	66	26	0	220	285	229	294	4.44e-22	97.1
MsG0580028553.01.T02	AT1G13960	56.667	60	25	1	220	278	409	468	1.86e-15	77.4
MsG0580028553.01.T02	AT5G07100	61.429	70	25	2	220	288	24	92	5.97e-22	92.4
MsG0580028553.01.T02	AT5G07100	56.667	60	25	1	220	278	141	200	4.78e-15	73.6
MsG0580028553.01.T02	AT5G07100	61.429	70	25	2	220	288	24	92	5.97e-22	92.4
MsG0580028553.01.T02	AT5G07100	56.667	60	25	1	220	278	141	200	4.78e-15	73.6
MsG0580028553.01.T02	AT5G07100	61.429	70	25	2	220	288	24	92	5.97e-22	92.4
MsG0580028553.01.T02	AT5G07100	56.667	60	25	1	220	278	141	200	4.78e-15	73.6
MsG0580028553.01.T02	AT5G07100	61.429	70	25	2	220	288	24	92	5.97e-22	92.4
MsG0580028553.01.T02	AT5G07100	56.667	60	25	1	220	278	141	200	4.78e-15	73.6
MsG0580028553.01.T02	AT1G13960	60.606	66	26	0	220	285	76	141	6.79e-22	95.1
MsG0580028553.01.T02	AT1G13960	56.667	60	25	1	220	278	256	315	4.44e-15	75.5
MsG0580028553.01.T02	AT5G07100	61.429	70	25	2	220	288	117	185	7.98e-22	94.4
MsG0580028553.01.T02	AT5G07100	56.667	60	25	1	220	278	234	293	4.32e-15	75.1
MsG0580028553.01.T02	AT2G38470	63.333	60	22	0	220	279	184	243	1.50e-21	95.5
MsG0580028553.01.T02	AT2G38470	56.667	60	25	1	220	278	362	421	3.11e-13	70.9
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	142	236	1.98e-21	95.1
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	341	419	1.11e-14	75.1
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	182	276	2.28e-21	95.1
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	381	459	1.16e-14	75.1
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	110	204	2.91e-21	94.7
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	309	387	1.39e-14	74.7
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	6	100	3.28e-21	93.6
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	205	283	1.40e-14	74.3
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	6	100	3.28e-21	93.6
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	205	283	1.40e-14	74.3
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	6	100	3.28e-21	93.6
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	205	283	1.40e-14	74.3
MsG0580028553.01.T02	AT4G26640	50.000	96	37	2	201	286	6	100	3.28e-21	93.6
MsG0580028553.01.T02	AT4G26640	48.148	81	39	2	220	299	205	283	1.40e-14	74.3
MsG0580028553.01.T02	AT2G03340	57.353	68	29	0	220	287	250	317	6.66e-21	93.6
MsG0580028553.01.T02	AT2G03340	56.667	60	25	1	220	278	415	474	2.15e-15	77.4
MsG0580028553.01.T02	AT2G37260	57.353	68	29	0	221	288	166	233	2.59e-20	91.3
MsG0580028553.01.T02	AT2G37260	57.353	68	29	0	221	288	86	153	2.78e-20	90.5
MsG0580028553.01.T02	AT2G37260	57.353	68	29	0	221	288	84	151	3.06e-20	90.5
MsG0580028553.01.T02	AT5G56270	59.375	64	26	0	220	283	273	336	6.47e-20	90.9
MsG0580028553.01.T02	AT5G56270	56.452	62	26	1	220	280	487	548	4.07e-15	76.6
MsG0580028553.01.T02	AT2G30250	47.059	102	48	1	218	319	164	259	7.54e-20	89.7
MsG0580028553.01.T02	AT2G30250	55.932	59	25	1	221	278	329	387	1.49e-13	71.2
MsG0580028553.01.T02	AT3G01080	60.000	60	24	0	220	279	205	264	8.57e-19	85.9
MsG0580028553.01.T02	AT2G04880	50.000	74	37	0	205	278	96	169	1.00e-18	87.0
MsG0580028553.01.T02	AT2G04880	51.667	60	28	1	220	278	307	366	1.36e-12	68.6
MsG0580028553.01.T02	AT2G04880	50.000	74	37	0	205	278	96	169	1.29e-18	86.7
MsG0580028553.01.T02	AT2G04880	51.667	60	28	1	220	278	283	342	1.51e-12	68.6
MsG0580028553.01.T02	AT4G26440	56.250	64	28	0	220	283	178	241	3.16e-18	85.9
MsG0580028553.01.T02	AT4G26440	53.623	69	28	2	220	287	372	437	3.72e-13	70.5
MsG0580028553.01.T02	AT3G01080	59.016	61	25	0	220	280	205	265	4.53e-18	85.1
MsG0580028553.01.T02	AT3G01080	58.333	60	24	1	220	278	344	403	1.26e-16	80.9
MsG0580028553.01.T02	AT3G01080	59.016	61	25	0	220	280	167	227	6.03e-18	84.3
MsG0580028553.01.T02	AT3G01080	58.333	60	24	1	220	278	306	365	1.76e-16	80.1
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	3.26e-15	77.4
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	3.45e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	3.68e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	3.68e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	3.89e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	4.08e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	4.08e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	4.08e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	390	470	4.09e-15	77.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	4.18e-15	77.0
MsG0580028553.01.T02	AT5G13080	53.968	63	28	1	220	281	67	129	4.52e-15	72.0
MsG0580028553.01.T02	AT4G12020	44.706	85	43	2	207	291	457	537	5.58e-15	76.6
MsG0580028553.01.T02	AT1G55600	53.125	64	29	1	218	280	305	368	3.41e-14	73.6
MsG0580028553.01.T02	AT1G29860	49.315	73	35	2	220	291	136	207	1.37e-13	70.5
MsG0580028553.01.T02	AT2G44745	38.655	119	54	3	176	275	83	201	1.58e-13	69.3
MsG0580028553.01.T02	AT1G64000	49.180	61	30	1	220	279	114	174	1.59e-12	65.9
MsG0580028553.01.T02	AT5G49520	53.226	62	28	1	218	278	219	280	2.20e-12	67.8
MsG0580028553.01.T02	AT2G46130	54.386	57	25	1	220	275	30	86	2.83e-12	63.2
MsG0580028553.01.T02	AT5G41570	50.877	57	27	1	220	275	98	154	4.12e-12	64.3
MsG0580028553.01.T02	AT4G18170	51.667	60	28	1	220	278	172	231	4.15e-12	66.6
MsG0580028553.01.T02	AT1G68150	49.180	61	29	2	220	278	235	295	1.18e-11	65.5
MsG0580028553.01.T02	AT4G30935	41.429	70	41	0	212	281	160	229	1.29e-11	65.9
MsG0580028553.01.T02	AT4G30935	40.541	74	43	1	221	293	332	405	8.51e-11	63.2
MsG0580028553.01.T02	AT1G80840	46.667	60	30	2	221	278	147	206	1.56e-11	64.7
MsG0580028553.01.T02	AT5G46350	50.000	60	29	1	220	278	183	242	2.22e-11	64.3
MsG0580028553.01.T02	AT4G39410	44.872	78	40	3	220	295	223	299	2.34e-11	64.3
MsG0580028553.01.T02	AT2G21900	48.333	60	30	1	220	278	109	168	2.58e-11	62.8
MsG0580028553.01.T02	AT5G15130	43.836	73	37	3	220	288	185	257	2.83e-11	64.7
MsG0580028553.01.T02	AT5G15130	43.836	73	37	3	220	288	227	299	2.90e-11	64.7
MsG0580028553.01.T02	AT2G47260	50.000	60	29	1	220	278	174	233	3.98e-11	63.5
MsG0580028553.01.T02	AT5G26170	50.877	57	27	1	220	275	113	169	4.26e-11	61.2
MsG0580028553.01.T02	AT3G62340	36.842	114	59	3	220	320	118	231	8.65e-11	62.0
MsG0580028553.01.T01	AT5G07100	61.429	70	25	2	126	194	24	92	2.64e-22	91.3
MsG0580028553.01.T01	AT5G07100	56.667	60	25	1	126	184	141	200	1.78e-15	73.2
MsG0580028553.01.T01	AT5G07100	61.429	70	25	2	126	194	24	92	2.64e-22	91.3
MsG0580028553.01.T01	AT5G07100	56.667	60	25	1	126	184	141	200	1.78e-15	73.2
MsG0580028553.01.T01	AT5G07100	61.429	70	25	2	126	194	24	92	2.64e-22	91.3
MsG0580028553.01.T01	AT5G07100	56.667	60	25	1	126	184	141	200	1.78e-15	73.2
MsG0580028553.01.T01	AT5G07100	61.429	70	25	2	126	194	24	92	2.64e-22	91.3
MsG0580028553.01.T01	AT5G07100	56.667	60	25	1	126	184	141	200	1.78e-15	73.2
MsG0580028553.01.T01	AT1G13960	60.606	66	26	0	126	191	229	294	3.10e-22	95.1
MsG0580028553.01.T01	AT1G13960	56.667	60	25	1	126	184	409	468	1.04e-15	76.3
MsG0580028553.01.T01	AT5G07100	61.429	70	25	2	126	194	117	185	3.84e-22	92.8
MsG0580028553.01.T01	AT5G07100	56.667	60	25	1	126	184	234	293	1.92e-15	74.3
MsG0580028553.01.T01	AT1G13960	60.606	66	26	0	126	191	76	141	3.99e-22	93.6
MsG0580028553.01.T01	AT1G13960	56.667	60	25	1	126	184	256	315	2.20e-15	74.7
MsG0580028553.01.T01	AT2G38470	63.333	60	22	0	126	185	184	243	8.16e-22	94.0
MsG0580028553.01.T01	AT2G38470	54.839	62	27	1	126	186	362	423	1.42e-13	69.7
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	142	236	1.18e-21	93.2
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	341	419	7.02e-15	73.6
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	182	276	1.35e-21	93.2
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	381	459	7.46e-15	73.6
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	110	204	1.59e-21	92.8
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	309	387	8.20e-15	73.6
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	6	100	1.93e-21	92.0
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	205	283	9.05e-15	73.2
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	6	100	1.93e-21	92.0
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	205	283	9.05e-15	73.2
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	6	100	1.93e-21	92.0
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	205	283	9.05e-15	73.2
MsG0580028553.01.T01	AT4G26640	50.000	96	37	2	107	192	6	100	1.93e-21	92.0
MsG0580028553.01.T01	AT4G26640	48.148	81	39	2	126	205	205	283	9.05e-15	73.2
MsG0580028553.01.T01	AT2G03340	57.353	68	29	0	126	193	250	317	3.76e-21	92.0
MsG0580028553.01.T01	AT2G03340	54.839	62	27	1	126	186	415	476	1.37e-15	75.9
MsG0580028553.01.T01	AT2G37260	57.353	68	29	0	127	194	166	233	1.17e-20	90.1
MsG0580028553.01.T01	AT2G37260	38.824	85	45	2	100	183	329	407	6.86e-11	61.6
MsG0580028553.01.T01	AT2G37260	57.353	68	29	0	127	194	86	153	1.20e-20	89.4
MsG0580028553.01.T01	AT2G37260	57.353	68	29	0	127	194	84	151	1.25e-20	89.4
MsG0580028553.01.T01	AT2G30250	47.059	102	48	1	124	225	164	259	1.94e-20	89.4
MsG0580028553.01.T01	AT2G30250	55.932	59	25	1	127	184	329	387	6.45e-14	70.5
MsG0580028553.01.T01	AT5G56270	64.407	59	21	0	126	184	273	331	3.03e-20	89.4
MsG0580028553.01.T01	AT5G56270	56.452	62	26	1	126	186	487	548	1.64e-15	75.5
MsG0580028553.01.T01	AT2G04880	50.000	74	37	0	111	184	96	169	2.35e-19	86.7
MsG0580028553.01.T01	AT2G04880	51.667	60	28	1	126	184	307	366	6.29e-13	67.8
MsG0580028553.01.T01	AT2G04880	50.000	74	37	0	111	184	96	169	2.40e-19	86.7
MsG0580028553.01.T01	AT2G04880	51.667	60	28	1	126	184	283	342	6.02e-13	67.8
MsG0580028553.01.T01	AT3G01080	60.000	60	24	0	126	185	205	264	8.52e-19	84.0
MsG0580028553.01.T01	AT4G26440	61.017	59	23	0	126	184	178	236	1.87e-18	84.3
MsG0580028553.01.T01	AT4G26440	53.623	69	28	2	126	193	372	437	1.94e-13	69.3
MsG0580028553.01.T01	AT3G01080	60.000	60	24	0	126	185	205	264	3.50e-18	83.2
MsG0580028553.01.T01	AT3G01080	58.333	60	24	1	126	184	344	403	9.61e-17	79.0
MsG0580028553.01.T01	AT3G01080	60.000	60	24	0	126	185	167	226	4.77e-18	82.8
MsG0580028553.01.T01	AT3G01080	58.333	60	24	1	126	184	306	365	1.33e-16	78.6
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.40e-15	75.5
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.54e-15	75.5
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.60e-15	75.5
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.60e-15	75.5
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.83e-15	75.1
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.83e-15	75.1
MsG0580028553.01.T01	AT5G13080	53.968	63	28	1	126	187	67	129	2.86e-15	70.9
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.87e-15	75.1
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.90e-15	75.1
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	2.91e-15	75.1
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	390	470	3.00e-15	75.1
MsG0580028553.01.T01	AT4G12020	44.706	85	43	2	113	197	457	537	3.81e-15	74.7
MsG0580028553.01.T01	AT1G55600	46.250	80	37	2	113	186	289	368	1.81e-14	72.4
MsG0580028553.01.T01	AT1G29860	49.315	73	35	2	126	197	136	207	6.82e-14	69.7
MsG0580028553.01.T01	AT2G44745	38.655	119	54	3	82	181	83	201	8.75e-14	68.6
MsG0580028553.01.T01	AT2G25000	32.653	98	60	3	95	190	76	169	2.84e-13	67.4
MsG0580028553.01.T01	AT2G25000	32.653	98	60	3	95	190	76	169	2.84e-13	67.4
MsG0580028553.01.T01	AT2G25000	32.653	98	60	3	95	190	98	191	4.94e-13	67.0
MsG0580028553.01.T01	AT2G25000	32.653	98	60	3	95	190	119	212	7.55e-13	66.6
MsG0580028553.01.T01	AT5G49520	53.226	62	28	1	124	184	219	280	8.75e-13	67.4
MsG0580028553.01.T01	AT4G04450	41.558	77	43	2	127	201	199	275	1.12e-12	67.0
MsG0580028553.01.T01	AT4G04450	41.558	77	43	2	127	201	293	369	1.62e-12	66.6
MsG0580028553.01.T01	AT1G64000	49.180	61	30	1	126	185	114	174	1.67e-12	64.7
MsG0580028553.01.T01	AT4G18170	51.667	60	28	1	126	184	172	231	2.46e-12	65.5
MsG0580028553.01.T01	AT2G46130	54.386	57	25	1	126	181	30	86	2.73e-12	62.0
MsG0580028553.01.T01	AT5G41570	50.877	57	27	1	126	181	98	154	3.43e-12	63.5
MsG0580028553.01.T01	AT1G68150	49.180	61	29	2	126	184	235	295	4.83e-12	65.1
MsG0580028553.01.T01	AT4G30935	37.209	86	54	0	102	187	144	229	5.34e-12	65.1
MsG0580028553.01.T01	AT4G30935	40.541	74	43	1	127	199	332	405	2.97e-11	62.8
MsG0580028553.01.T01	AT5G15130	43.836	73	37	3	126	194	185	257	7.64e-12	64.7
MsG0580028553.01.T01	AT5G15130	43.836	73	37	3	126	194	227	299	7.82e-12	64.7
MsG0580028553.01.T01	AT1G80840	46.667	60	30	2	127	184	147	206	9.06e-12	63.9
MsG0580028553.01.T01	AT4G39410	44.872	78	40	3	126	201	223	299	1.56e-11	63.2
MsG0580028553.01.T01	AT5G46350	50.000	60	29	1	126	184	183	242	1.73e-11	63.2
MsG0580028553.01.T01	AT2G21900	44.118	68	37	1	118	184	101	168	2.30e-11	61.6
MsG0580028553.01.T01	AT2G47260	50.000	60	29	1	126	184	174	233	2.67e-11	62.8
MsG0580028553.01.T01	AT3G62340	36.842	114	59	3	126	226	118	231	3.29e-11	62.0
MsG0580028553.01.T01	AT5G26170	50.877	57	27	1	126	181	113	169	3.52e-11	60.5

Find 35 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTGTTCTTCGTTCGAAAT+TGG	0.227728	5:+82188524	MsG0580028553.01.T02:CDS
TAGTCCATTGGAAATTCTTC+TGG	0.229708	5:-82188040	None:intergenic
ATTCTTGCTGAGAGCTCATT+TGG	0.246775	5:+82188254	MsG0580028553.01.T02:CDS
TTGGATTTGAGGCATGAAAT+TGG	0.257475	5:+82188543	MsG0580028553.01.T02:CDS
ATCATGATGATGGGTATAAT+TGG	0.315840	5:+82188688	MsG0580028553.01.T02:CDS
GATCTCCTCAATTTCTTCCT+TGG	0.315896	5:+82188429	MsG0580028553.01.T02:CDS
ATCATTTGGAAGTGAACAAT+TGG	0.346812	5:+82188959	MsG0580028553.01.T02:CDS
ATTGATACTGAAGACTTTGC+AGG	0.359791	5:-82188996	None:intergenic
GACTTGCAAGATCAATCATT+TGG	0.392633	5:+82188945	MsG0580028553.01.T02:CDS
TTCTTGCTGAGAGCTCATTT+GGG	0.400134	5:+82188255	MsG0580028553.01.T02:CDS
TTATCAAGACAGATTGATCT+TGG	0.404285	5:+82188396	MsG0580028553.01.T02:CDS
ATAGTTGAGAGAACTGTAGA+TGG	0.406452	5:+82188798	MsG0580028553.01.T02:CDS
TATGAAGATAAAGTAGCAAA+AGG	0.433124	5:+82188717	MsG0580028553.01.T02:CDS
TGATTGATCTTGCAAGTCAA+TGG	0.446931	5:-82188940	None:intergenic
AAAATCAGTCTTTGATAAGA+AGG	0.457061	5:+82188661	MsG0580028553.01.T02:CDS
GATGTAGCTATTGAAATTAA+TGG	0.458940	5:+82188585	MsG0580028553.01.T02:CDS
TTTCAATCTGATTATAGTAA+TGG	0.460671	5:+82188630	MsG0580028553.01.T02:CDS
CACAAGAAATCATACTCAAT+AGG	0.466199	5:-82188347	None:intergenic
ACAAAGGTACACATAATCAT+TGG	0.470196	5:+82188841	MsG0580028553.01.T02:CDS
TTCCTCTTCATTGTAGACTT+AGG	0.480559	5:-82188867	None:intergenic
AGAAGGATGAATCATGATGA+TGG	0.481196	5:+82188678	MsG0580028553.01.T02:CDS
AGTCCATTGGAAATTCTTCT+GGG	0.484043	5:-82188039	None:intergenic
TTCGTTCGAAATTGGATTTG+AGG	0.491204	5:+82188532	MsG0580028553.01.T02:CDS
GATTGATCTTGCAAGTCAAT+GGG	0.496257	5:-82188939	None:intergenic
GAAATCATACTCAATAGGTC+TGG	0.504110	5:-82188342	None:intergenic
ATTGATCTTCATCAATTGAA+CGG	0.555673	5:-82188460	None:intergenic
AAATTCCAAGGAAGAAATTG+AGG	0.559723	5:-82188434	None:intergenic
GAAGGATGAATCATGATGAT+GGG	0.572110	5:+82188679	MsG0580028553.01.T02:CDS
ATGGGACCAATTGTCTCTGA+AGG	0.591030	5:-82188921	None:intergenic
TGCCTAAGTCTACAATGAAG+AGG	0.601126	5:+82188865	MsG0580028553.01.T02:CDS
ATTGAACGGTGAAAATTCCA+AGG	0.604070	5:-82188446	None:intergenic
ATGCCCAGAAGAATTTCCAA+TGG	0.634558	5:+82188036	MsG0580028553.01.T02:CDS
CAAAATCATTATTTACACAG+TGG	0.687753	5:+82188173	MsG0580028553.01.T02:CDS
ATCGTCGAGACATTATACAA+AGG	0.713305	5:+82188825	MsG0580028553.01.T02:CDS
GAAGCTATTATAAATGTACG+TGG	0.742371	5:+82188754	MsG0580028553.01.T02:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGATGTTATTTTTGAAAAAA+TGG	+	Chr5:82188217-82188236	MsG0580028553.01.T02:CDS	15.0%
!!	TTTCAATCTGATTATAGTAA+TGG	+	Chr5:82188630-82188649	MsG0580028553.01.T02:CDS	20.0%
!!!	AAAAATCAATGGAGAATTTT+AGG	+	Chr5:82188069-82188088	MsG0580028553.01.T02:CDS	20.0%
!!!	ATTTTCTTCACAAAACAATT+AGG	-	Chr5:82188780-82188799	None:intergenic	20.0%
!	AAAATCAGTCTTTGATAAGA+AGG	+	Chr5:82188661-82188680	MsG0580028553.01.T02:CDS	25.0%
!	ATTGATCTTCATCAATTGAA+CGG	-	Chr5:82188463-82188482	None:intergenic	25.0%
!	CAAAATCATTATTTACACAG+TGG	+	Chr5:82188173-82188192	MsG0580028553.01.T02:CDS	25.0%
!	GACTAATACAGAAAAATCAA+TGG	+	Chr5:82188058-82188077	MsG0580028553.01.T02:CDS	25.0%
!	GATGTAGCTATTGAAATTAA+TGG	+	Chr5:82188585-82188604	MsG0580028553.01.T02:CDS	25.0%
!	TATGAAGATAAAGTAGCAAA+AGG	+	Chr5:82188717-82188736	MsG0580028553.01.T02:CDS	25.0%
!	TGAAAAAATGGAAACTTTCA+AGG	+	Chr5:82188229-82188248	MsG0580028553.01.T02:CDS	25.0%
!!	ATTTTTCTGTATTAGTCCAT+TGG	-	Chr5:82188055-82188074	None:intergenic	25.0%
!!	TCTTCATTCAATCATCTTTT+TGG	+	Chr5:82188135-82188154	MsG0580028553.01.T02:CDS	25.0%
!!!	TTATCAAAGACTGATTTTGT+TGG	-	Chr5:82188659-82188678	None:intergenic	25.0%
	AAATTCCAAGGAAGAAATTG+AGG	-	Chr5:82188437-82188456	None:intergenic	30.0%
	ATCATTTGGAAGTGAACAAT+TGG	+	Chr5:82188959-82188978	MsG0580028553.01.T02:CDS	30.0%
	CACAAGAAATCATACTCAAT+AGG	-	Chr5:82188350-82188369	None:intergenic	30.0%
	GAAGCTATTATAAATGTACG+TGG	+	Chr5:82188754-82188773	MsG0580028553.01.T02:CDS	30.0%
	TATTGTTCTTCGTTCGAAAT+TGG	+	Chr5:82188524-82188543	MsG0580028553.01.T02:CDS	30.0%
	TTATCAAGACAGATTGATCT+TGG	+	Chr5:82188396-82188415	MsG0580028553.01.T02:CDS	30.0%
!	ACAAAGGTACACATAATCAT+TGG	+	Chr5:82188841-82188860	MsG0580028553.01.T02:CDS	30.0%
!!	ATCATGATGATGGGTATAAT+TGG	+	Chr5:82188688-82188707	MsG0580028553.01.T02:CDS	30.0%
!!	TCAATCATCTTTTTGGAAAG+TGG	+	Chr5:82188142-82188161	MsG0580028553.01.T02:CDS	30.0%
!!	TTTTTCGTTCGAAGCTTTAT+CGG	-	Chr5:82188322-82188341	None:intergenic	30.0%
!!!	GGTGTTTTTTCAAGTATCAT+TGG	+	Chr5:82188090-82188109	MsG0580028553.01.T02:CDS	30.0%
	AGAAGGATGAATCATGATGA+TGG	+	Chr5:82188678-82188697	MsG0580028553.01.T02:CDS	35.0%
	AGTCCATTGGAAATTCTTCT+GGG	-	Chr5:82188042-82188061	None:intergenic	35.0%
	ATAGTTGAGAGAACTGTAGA+TGG	+	Chr5:82188798-82188817	MsG0580028553.01.T02:CDS	35.0%
	ATCGTCGAGACATTATACAA+AGG	+	Chr5:82188825-82188844	MsG0580028553.01.T02:CDS	35.0%
	ATTGAACGGTGAAAATTCCA+AGG	-	Chr5:82188449-82188468	None:intergenic	35.0%
	ATTGATACTGAAGACTTTGC+AGG	-	Chr5:82188999-82189018	None:intergenic	35.0%
	GAAATCATACTCAATAGGTC+TGG	-	Chr5:82188345-82188364	None:intergenic	35.0%
	GAAGGATGAATCATGATGAT+GGG	+	Chr5:82188679-82188698	MsG0580028553.01.T02:CDS	35.0%
	GACTTGCAAGATCAATCATT+TGG	+	Chr5:82188945-82188964	MsG0580028553.01.T02:CDS	35.0%
	GATTGATCTTGCAAGTCAAT+GGG	-	Chr5:82188942-82188961	None:intergenic	35.0%
	TAGTCCATTGGAAATTCTTC+TGG	-	Chr5:82188043-82188062	None:intergenic	35.0%
	TGATTGATCTTGCAAGTCAA+TGG	-	Chr5:82188943-82188962	None:intergenic	35.0%
	TTCCTCTTCATTGTAGACTT+AGG	-	Chr5:82188870-82188889	None:intergenic	35.0%
	TTCGTTCGAAATTGGATTTG+AGG	+	Chr5:82188532-82188551	MsG0580028553.01.T02:CDS	35.0%
!	CTTTTACCTTCAGAGACAAT+TGG	+	Chr5:82188915-82188934	MsG0580028553.01.T02:CDS	35.0%
!	TTGGATTTGAGGCATGAAAT+TGG	+	Chr5:82188543-82188562	MsG0580028553.01.T02:CDS	35.0%
	ATGCCCAGAAGAATTTCCAA+TGG	+	Chr5:82188036-82188055	MsG0580028553.01.T02:CDS	40.0%
	ATTCTTGCTGAGAGCTCATT+TGG	+	Chr5:82188254-82188273	MsG0580028553.01.T02:CDS	40.0%
	GATCTCCTCAATTTCTTCCT+TGG	+	Chr5:82188429-82188448	MsG0580028553.01.T02:CDS	40.0%
	TGCCTAAGTCTACAATGAAG+AGG	+	Chr5:82188865-82188884	MsG0580028553.01.T02:CDS	40.0%
	TTCTTGCTGAGAGCTCATTT+GGG	+	Chr5:82188255-82188274	MsG0580028553.01.T02:CDS	40.0%
	ATGGGACCAATTGTCTCTGA+AGG	-	Chr5:82188924-82188943	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	82188036	82189022	82188036	ID=MsG0580028553.01;Name=MsG0580028553.01
Chr5	mRNA	82188036	82189022	82188036	ID=MsG0580028553.01.T01;Parent=MsG0580028553.01;Name=MsG0580028553.01.T01;_AED=0.15;_eAED=0.15;_QI=0\|0\|0\|1\|0\|0\|2\|0\|234
Chr5	exon	82188036	82188194	82188036	ID=MsG0580028553.01.T01:exon:33083;Parent=MsG0580028553.01.T01
Chr5	exon	82188477	82189022	82188477	ID=MsG0580028553.01.T01:exon:33084;Parent=MsG0580028553.01.T01
Chr5	CDS	82188036	82188194	82188036	ID=MsG0580028553.01.T01:cds;Parent=MsG0580028553.01.T01
Chr5	CDS	82188477	82189022	82188477	ID=MsG0580028553.01.T01:cds;Parent=MsG0580028553.01.T01
Chr5	mRNA	82188036	82189022	82188036	ID=MsG0580028553.01.T02;Parent=MsG0580028553.01;Name=MsG0580028553.01.T02;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|328
Chr5	exon	82188036	82189022	82188036	ID=MsG0580028553.01.T02:exon:33085;Parent=MsG0580028553.01.T02
Chr5	CDS	82188036	82189022	82188036	ID=MsG0580028553.01.T02:cds;Parent=MsG0580028553.01.T02
Chr5	mRNA	82188817	82188882	82188817	ID=MsG0580028553.01.T03;Parent=MsG0580028553.01;Name=MsG0580028553.01.T03;_AED=0.47;_eAED=1.00;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|21
Chr5	exon	82188817	82188882	82188817	ID=MsG0580028553.01.T03:exon:33086;Parent=MsG0580028553.01.T03
Chr5	CDS	82188817	82188882	82188817	ID=MsG0580028553.01.T03:cds;Parent=MsG0580028553.01.T03

Gene Sequence

>MsG0580028553.01.T02

ATGCCCAGAAGAATTTCCAATGGACTAATACAGAAAAATCAATGGAGAATTTTAGGTGTTTTTTCAAGTATCATTGGATTCACTTCTTATGCATTCAGTTCTTCATTCAATCATCTTTTTGGAAAGTGGAACTTCATCAAAATCATTATTTACACAGTGGTAAGTTTCAGTATTAGTAGCATGATGTTATTTTTGAAAAAATGGAAACTTTCAAGGAGATTCTTGCTGAGAGCTCATTTGGGTGTTCTTGTTTTGTTGCTCACATCTTTGTACTCTTTTGTCTCCGATAAAGCTTCGAACGAAAAACCAGACCTATTGAGTATGATTTCTTGTGTTGCTTTTGCTTTAATGTCTTTTTGCTTATCAAGACAGATTGATCTTGGTTTTGAATCTGATCTCCTCAATTTCTTCCTTGGAATTTTCACCGTTCAATTGATGAAGATCAATTTGATTCTATCTATTGTTGCTGCAATCTTATGTTATTCTCTTATTGTTCTTCGTTCGAAATTGGATTTGAGGCATGAAATTGGAACTTCAAGAGTGCAAGATGATGTAGCTATTGAAATTAATGGTGTAGATAACGATAGAAATGAATTTCAATCTGATTATAGTAATGGTAGCCAACAAAATCAGTCTTTGATAAGAAGGATGAATCATGATGATGGGTATAATTGGAAAAAGTATGAAGATAAAGTAGCAAAAGGAAGTGAAAATCAAAGAAGCTATTATAAATGTACGTGGCCTAATTGTTTTGTGAAGAAAATAGTTGAGAGAACTGTAGATGGAGAAATCGTCGAGACATTATACAAAGGTACACATAATCATTGGATGCCTAAGTCTACAATGAAGAGGAATTCATCATCTGAGTATCTTTATTCACTTTTACCTTCAGAGACAATTGGTCCCATTGACTTGCAAGATCAATCATTTGGAAGTGAACAATTGGATTCTGATGAAGAACCTGCAAAGTCTTCAGTATCAATATGA

>MsG0580028553.01.T01

ATGCCCAGAAGAATTTCCAATGGACTAATACAGAAAAATCAATGGAGAATTTTAGGTGTTTTTTCAAGTATCATTGGATTCACTTCTTATGCATTCAGTTCTTCATTCAATCATCTTTTTGGAAAGTGGAACTTCATCAAAATCATTATTTACACAGTGATCAATTTGATTCTATCTATTGTTGCTGCAATCTTATGTTATTCTCTTATTGTTCTTCGTTCGAAATTGGATTTGAGGCATGAAATTGGAACTTCAAGAGTGCAAGATGATGTAGCTATTGAAATTAATGGTGTAGATAACGATAGAAATGAATTTCAATCTGATTATAGTAATGGTAGCCAACAAAATCAGTCTTTGATAAGAAGGATGAATCATGATGATGGGTATAATTGGAAAAAGTATGAAGATAAAGTAGCAAAAGGAAGTGAAAATCAAAGAAGCTATTATAAATGTACGTGGCCTAATTGTTTTGTGAAGAAAATAGTTGAGAGAACTGTAGATGGAGAAATCGTCGAGACATTATACAAAGGTACACATAATCATTGGATGCCTAAGTCTACAATGAAGAGGAATTCATCATCTGAGTATCTTTATTCACTTTTACCTTCAGAGACAATTGGTCCCATTGACTTGCAAGATCAATCATTTGGAAGTGAACAATTGGATTCTGATGAAGAACCTGCAAAGTCTTCAGTATCAATATGA

>MsG0580028553.01.T03

ATGGAGAAATCGTCGAGACATTATACAAAGGTACACATAATCATTGGATGCCTAAGTCTACAATGA

Protein sequence

>MsG0580028553.01.T02

MPRRISNGLIQKNQWRILGVFSSIIGFTSYAFSSSFNHLFGKWNFIKIIIYTVVSFSISSMMLFLKKWKLSRRFLLRAHLGVLVLLLTSLYSFVSDKASNEKPDLLSMISCVAFALMSFCLSRQIDLGFESDLLNFFLGIFTVQLMKINLILSIVAAILCYSLIVLRSKLDLRHEIGTSRVQDDVAIEINGVDNDRNEFQSDYSNGSQQNQSLIRRMNHDDGYNWKKYEDKVAKGSENQRSYYKCTWPNCFVKKIVERTVDGEIVETLYKGTHNHWMPKSTMKRNSSSEYLYSLLPSETIGPIDLQDQSFGSEQLDSDEEPAKSSVSI*

>MsG0580028553.01.T01

MPRRISNGLIQKNQWRILGVFSSIIGFTSYAFSSSFNHLFGKWNFIKIIIYTVINLILSIVAAILCYSLIVLRSKLDLRHEIGTSRVQDDVAIEINGVDNDRNEFQSDYSNGSQQNQSLIRRMNHDDGYNWKKYEDKVAKGSENQRSYYKCTWPNCFVKKIVERTVDGEIVETLYKGTHNHWMPKSTMKRNSSSEYLYSLLPSETIGPIDLQDQSFGSEQLDSDEEPAKSSVSI*

>MsG0580028553.01.T03

MEKSSRHYTKVHIIIGCLSLQ*