AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028566.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028566.01.T01 MTR_5g074600 96.657 359 9 1 1 356 1 359 0.0 715
MsG0580028566.01.T01 MTR_5g074680 84.123 359 53 2 1 355 1 359 0.0 622
MsG0580028566.01.T01 MTR_5g074580 80.833 360 65 2 1 356 1 360 0.0 594
MsG0580028566.01.T01 MTR_5g074540 74.930 359 69 2 1 356 1 341 0.0 543
MsG0580028566.01.T01 MTR_5g074610 69.359 359 74 4 1 356 1 326 1.15e-179 501
MsG0580028566.01.T01 MTR_4g079160 61.111 360 131 5 1 356 1 355 6.38e-159 449
MsG0580028566.01.T01 MTR_4g033200 49.863 365 172 6 1 356 1 363 1.18e-115 340
MsG0580028566.01.T01 MTR_4g033015 49.589 365 173 6 1 356 1 363 2.54e-114 337
MsG0580028566.01.T01 MTR_7g014570 47.564 349 173 6 11 356 11 352 7.07e-113 332
MsG0580028566.01.T01 MTR_4g088200 47.592 353 176 6 11 356 11 361 1.59e-112 332
MsG0580028566.01.T01 MTR_4g033150 49.577 355 168 7 11 356 11 363 1.01e-109 325
MsG0580028566.01.T01 MTR_7g014510 44.602 352 184 6 11 356 11 357 5.95e-108 320
MsG0580028566.01.T01 MTR_4g088190 49.157 356 169 6 11 356 11 364 1.17e-107 320
MsG0580028566.01.T01 MTR_1g076940 44.780 364 190 7 1 356 1 361 3.36e-106 316
MsG0580028566.01.T01 MTR_4g088345 45.556 360 188 5 1 356 1 356 1.43e-104 311
MsG0580028566.01.T01 MTR_7g024500 43.978 357 188 6 1 354 1 348 2.94e-104 310
MsG0580028566.01.T01 MTR_7g024520 44.538 357 186 6 1 354 1 348 1.73e-103 308
MsG0580028566.01.T01 MTR_4g033170 48.011 352 172 6 11 356 11 357 1.31e-99 299
MsG0580028566.01.T01 MTR_4g033085 45.869 351 181 5 11 356 11 357 6.34e-95 287
MsG0580028566.01.T01 MTR_4g088340 44.214 337 180 5 24 356 1 333 3.67e-94 284
MsG0580028566.01.T01 MTR_4g038440 41.379 348 195 6 14 356 25 368 2.83e-84 260
MsG0580028566.01.T01 MTR_0079s0070 41.716 338 188 6 24 356 1 334 1.42e-81 252
MsG0580028566.01.T01 MTR_3g083620 39.104 335 198 4 20 352 27 357 1.47e-80 250
MsG0580028566.01.T01 MTR_0017s0100 40.805 348 197 6 14 356 25 368 6.67e-80 249
MsG0580028566.01.T01 MTR_0794s0020 42.678 239 130 5 12 244 12 249 1.25e-62 200
MsG0580028566.01.T01 MTR_1g076930 41.176 238 136 3 24 258 1 237 4.04e-57 186
MsG0580028566.01.T01 MTR_7g118320 34.472 322 197 10 30 341 30 347 6.87e-54 181
MsG0580028566.01.T01 MTR_7g118300 34.268 321 200 8 31 345 47 362 2.64e-51 175
MsG0580028566.01.T01 MTR_4g038440 38.306 248 148 3 14 258 25 270 6.08e-51 171
MsG0580028566.01.T01 MTR_8g068870 32.087 321 204 9 31 341 32 348 2.19e-49 169
MsG0580028566.01.T01 MTR_8g024160 37.308 260 158 5 98 355 71 327 8.74e-49 167
MsG0580028566.01.T01 MTR_8g068750 32.188 320 205 7 31 341 46 362 1.00e-48 168
MsG0580028566.01.T01 MTR_0591s0010 31.962 316 203 7 31 337 46 358 4.34e-48 167
MsG0580028566.01.T01 MTR_3g092900 31.776 321 205 9 31 342 34 349 3.68e-47 164
MsG0580028566.01.T01 MTR_1g096310 29.179 329 218 7 26 343 34 358 3.81e-47 164
MsG0580028566.01.T01 MTR_5g098170 31.250 320 211 5 31 343 33 350 1.11e-46 162
MsG0580028566.01.T01 MTR_7g012040 30.154 325 215 7 26 342 33 353 4.12e-45 159
MsG0580028566.01.T01 MTR_8g068690 31.646 316 204 7 31 337 45 357 6.32e-45 158
MsG0580028566.01.T01 MTR_7g012070 31.047 277 181 5 73 343 89 361 1.68e-44 157
MsG0580028566.01.T01 MTR_7g012090 31.522 276 179 5 73 342 89 360 5.89e-44 155
MsG0580028566.01.T01 MTR_6g078850 29.814 322 212 8 31 341 50 368 6.91e-44 158
MsG0580028566.01.T01 MTR_7g011990 29.110 292 197 6 71 355 78 366 1.57e-43 154
MsG0580028566.01.T01 MTR_2g055940 31.365 271 174 6 91 355 98 362 3.80e-43 153
MsG0580028566.01.T01 MTR_3g021440 31.173 324 210 8 28 343 38 356 2.62e-42 151
MsG0580028566.01.T01 MTR_7g011900 32.258 248 164 3 98 343 111 356 9.66e-42 150
MsG0580028566.01.T01 MTR_1g036460 29.730 370 235 11 1 355 10 369 1.20e-41 149
MsG0580028566.01.T01 MTR_1g036490 29.759 373 231 13 1 355 10 369 1.88e-41 149
MsG0580028566.01.T01 MTR_1g036510 29.930 284 192 5 64 343 89 369 1.04e-39 144
MsG0580028566.01.T01 MTR_2g072790 31.761 318 205 8 31 341 27 339 6.57e-39 142
MsG0580028566.01.T01 MTR_7g012020 26.923 338 237 7 26 355 33 368 1.56e-38 141
MsG0580028566.01.T01 MTR_3g021430 32.114 246 163 4 98 342 100 342 6.19e-37 137
MsG0580028566.01.T01 MTR_8g068570 40.964 166 91 4 161 323 156 317 1.42e-34 129
MsG0580028566.01.T01 MTR_8g068730 27.812 320 187 7 31 341 46 330 2.46e-34 129
MsG0580028566.01.T01 MTR_3g096050 27.129 317 220 7 32 341 31 343 1.01e-31 122
MsG0580028566.01.T01 MTR_3g096070 26.899 316 199 8 32 341 31 320 5.95e-28 112
MsG0580028566.01.T01 MTR_8g068600 27.826 230 154 7 39 259 28 254 1.17e-23 99.4
MsG0580028566.01.T01 MTR_7g012020 27.358 212 146 4 55 262 68 275 8.32e-23 97.4
MsG0580028566.01.T01 MTR_8g068620 38.053 113 69 1 149 261 2 113 5.02e-22 90.1
MsG0580028566.01.T01 MTR_8g068690 27.500 240 162 7 31 261 45 281 1.20e-21 94.0
MsG0580028566.01.T01 MTR_1g050220 31.373 153 94 2 194 346 152 293 5.92e-21 92.0
MsG0580028566.01.T01 MTR_1g036510 28.141 199 137 4 64 258 89 285 8.80e-20 88.6
MsG0580028566.01.T01 MTR_8g068740 25.498 251 143 7 31 272 46 261 2.73e-19 87.0
MsG0580028566.01.T01 MTR_3g096050 26.724 232 162 5 32 258 31 259 6.07e-18 82.8
MsG0580028566.01.T01 MTR_4g025570 44.211 95 49 3 260 352 122 214 2.60e-17 80.1
MsG0580028566.01.T01 MTR_7g012020 26.667 180 124 4 55 230 68 243 3.58e-15 74.7
MsG0580028566.01.T01 MTR_8g068760 43.529 85 46 2 258 341 135 218 2.50e-14 72.0
MsG0580028566.01.T01 MTR_1g036510 26.471 170 119 4 64 229 89 256 4.35e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028566.01.T01 AT4G35160 36.512 367 213 9 5 355 18 380 2.15e-67 217
MsG0580028566.01.T01 AT4G35150 33.516 364 187 8 6 355 1 323 2.69e-59 194
MsG0580028566.01.T01 AT1G51990 34.251 327 199 8 43 356 39 362 1.80e-52 178
MsG0580028566.01.T01 AT1G51990 34.251 327 199 8 43 356 39 362 2.14e-52 177
MsG0580028566.01.T01 AT5G54160 33.750 320 198 8 31 342 34 347 3.33e-52 177
MsG0580028566.01.T01 AT3G53140 33.025 324 198 9 31 341 27 344 9.75e-45 157
MsG0580028566.01.T01 AT1G33030 31.638 354 212 12 1 342 1 336 9.32e-44 154
MsG0580028566.01.T01 AT1G77520 30.084 359 225 9 14 355 31 380 2.39e-43 154
MsG0580028566.01.T01 AT5G53810 30.904 343 218 8 29 355 38 377 1.58e-37 139
MsG0580028566.01.T01 AT1G63140 31.988 322 203 6 37 355 72 380 3.64e-37 138
MsG0580028566.01.T01 AT1G63140 31.988 322 203 6 37 355 92 400 4.58e-37 138
MsG0580028566.01.T01 AT1G77530 30.994 342 214 8 31 355 44 380 1.38e-36 136
MsG0580028566.01.T01 AT1G62900 38.049 205 125 2 152 355 1 204 7.44e-33 122
MsG0580028566.01.T01 AT5G37170 33.173 208 134 4 150 355 29 233 6.62e-32 120
MsG0580028566.01.T01 AT5G37170 33.173 208 134 4 150 355 129 333 2.11e-31 121
MsG0580028566.01.T01 AT1G21130 27.660 329 212 8 31 342 40 359 5.61e-31 121
MsG0580028566.01.T01 AT1G21100 27.052 329 214 8 31 342 40 359 8.67e-31 120
MsG0580028566.01.T01 AT1G21110 27.301 326 209 10 31 338 40 355 5.16e-29 115
MsG0580028566.01.T01 AT1G76790 25.915 328 211 8 31 337 30 346 6.97e-29 115
MsG0580028566.01.T01 AT5G37170 33.871 186 120 3 172 355 1 185 8.05e-29 110
MsG0580028566.01.T01 AT1G21120 26.748 329 215 8 31 342 40 359 2.03e-28 114
MsG0580028566.01.T01 AT1G21120 26.748 329 215 8 31 342 88 407 4.20e-28 114
MsG0580028566.01.T01 AT1G33030 28.731 268 165 9 1 258 1 252 9.45e-20 88.2
MsG0580028566.01.T01 AT1G33030 28.731 268 165 9 1 258 1 252 1.42e-19 87.8
MsG0580028566.01.T01 AT1G63140 28.125 224 146 5 37 258 92 302 9.98e-15 74.3

Find 71 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAACCTGCTCTATATTT+TGG 0.103265 5:-82494723 None:intergenic
AAATAAATGTTGTTGATAAA+TGG 0.220916 5:-82497577 None:intergenic
ATAGACCTTAAGCCTAAAAT+TGG 0.263706 5:-82497521 None:intergenic
GTTAAATAAGTTGTTAATTT+CGG 0.270800 5:-82495199 None:intergenic
TGCCATGGATTCATCATAAC+TGG 0.291051 5:-82495087 None:intergenic
CCATAGTTGTGTATGATATC+TGG 0.318510 5:-82494838 None:intergenic
GATCTCCCACACGTTGTTGA+TGG 0.330428 5:+82495384 MsG0580028566.01.T01:CDS
CATGGCATCAATTCTCTACT+TGG 0.342461 5:+82495103 MsG0580028566.01.T01:CDS
CTCCATTTGTGGGAATGATT+TGG 0.349338 5:-82495349 None:intergenic
ATTCAAGTAGCAAGTAAGTA+TGG 0.350295 5:+82494696 MsG0580028566.01.T01:exon
GTTGATGTTGGAGGAGGAAC+AGG 0.362777 5:+82495306 MsG0580028566.01.T01:CDS
GCTTTGAGCTTTGAGCAAAT+TGG 0.386923 5:-82494746 None:intergenic
TTGATGATGGCCCTACTAAC+AGG 0.393845 5:+82497428 MsG0580028566.01.T01:CDS
CAACTTATTTAACGAGTCAA+TGG 0.405210 5:+82495209 MsG0580028566.01.T01:CDS
TCGATTAGTATCCGACTTGC+TGG 0.408545 5:+82495242 MsG0580028566.01.T01:CDS
CCAGATATCATACACAACTA+TGG 0.414017 5:+82494838 MsG0580028566.01.T01:CDS
CTTCTTTGATATGTTGATGA+TGG 0.415723 5:+82497415 MsG0580028566.01.T01:CDS
TGCCAAATCATTCCCACAAA+TGG 0.419064 5:+82495347 MsG0580028566.01.T01:CDS
GTTCAATGAAGTTGAGTCAT+TGG 0.427532 5:+82495284 MsG0580028566.01.T01:CDS
TGGAGGAGGAACAGGGACTA+TGG 0.434351 5:+82495314 MsG0580028566.01.T01:CDS
TGCATCTAAACTCTTACTTA+AGG 0.444246 5:+82495020 MsG0580028566.01.T01:CDS
ATGAAATTGATTTCTTTAGC+TGG 0.445713 5:+82497476 MsG0580028566.01.T01:CDS
CTTAAATGTGTAGTTGACTT+AGG 0.449531 5:+82494811 MsG0580028566.01.T01:CDS
AGTGAGAAGCTAAGATATGT+TGG 0.452323 5:+82495417 MsG0580028566.01.T01:CDS
AACACAATGCACTCCATTTG+TGG 0.453290 5:-82495360 None:intergenic
GTTGAGTCATTGGTTGATGT+TGG 0.468409 5:+82495294 MsG0580028566.01.T01:CDS
AAGAGAGAAACGAGAAGGAA+TGG 0.486925 5:+82497453 MsG0580028566.01.T01:CDS
CACGTTGTTGATGGCTTGCA+AGG 0.487896 5:+82495393 MsG0580028566.01.T01:CDS
CGACACATGGGATGTCAACA+TGG 0.498114 5:+82495157 MsG0580028566.01.T01:CDS
AAGGATGCAATATCAAAGAA+AGG 0.498497 5:+82497320 MsG0580028566.01.T01:CDS
TGCAGTGGATATTGCATGAC+TGG 0.504653 5:+82497261 MsG0580028566.01.T01:intron
ACGTATGGTGCCACACTCAT+TGG 0.505915 5:-82495048 None:intergenic
TCAAAGCTCAAAGCCATGTA+TGG 0.509812 5:+82494755 MsG0580028566.01.T01:CDS
TTGACCATTGCGTGCACGTA+TGG 0.510989 5:-82495063 None:intergenic
AAGTAAGAGTTTAGATGCAT+CGG 0.512463 5:-82495016 None:intergenic
ACACAATGCACTCCATTTGT+GGG 0.515239 5:-82495359 None:intergenic
TAAACATGTGATTCCATACA+TGG 0.516450 5:-82494768 None:intergenic
TTCAACAAAATTGCATCTGC+CGG 0.518520 5:-82495465 None:intergenic
ATGGTGTTGGATTGTGAGAA+GGG 0.533222 5:+82497362 MsG0580028566.01.T01:CDS
AGGATGCAATATCAAAGAAA+GGG 0.541489 5:+82497321 MsG0580028566.01.T01:CDS
GGTTATCATAGACATGGTGT+TGG 0.548752 5:+82497349 MsG0580028566.01.T01:CDS
TGTTGGATTGTGAGAAGGGA+GGG 0.561509 5:+82497366 MsG0580028566.01.T01:CDS
AACACATTTGAGACGACACA+TGG 0.563279 5:+82495144 MsG0580028566.01.T01:CDS
GGAATCCCAAAATATAGAGC+AGG 0.563858 5:+82494717 MsG0580028566.01.T01:CDS
TGCAAGCCATCAACAACGTG+TGG 0.566861 5:-82495390 None:intergenic
GAGTCATTGGTTGATGTTGG+AGG 0.570761 5:+82495297 MsG0580028566.01.T01:CDS
CAAGTAGAGAATTGATGCCA+TGG 0.571375 5:-82495102 None:intergenic
ATCCAGTTATGATGAATCCA+TGG 0.571870 5:+82495085 MsG0580028566.01.T01:CDS
ATGCGAATCATGACTCACTC+TGG 0.576549 5:+82494937 MsG0580028566.01.T01:CDS
AACAAAATTGCATCTGCCGG+CGG 0.579483 5:-82495462 None:intergenic
AACTACACATTTAAGTGACA+TGG 0.582842 5:-82494803 None:intergenic
GCATCAAGCGATGGATGCCA+TGG 0.585140 5:-82494918 None:intergenic
GAGAAGCTAAGATATGTTGG+TGG 0.598262 5:+82495420 MsG0580028566.01.T01:CDS
TCATGATTCGCATCAAGCGA+TGG 0.602700 5:-82494927 None:intergenic
CATGGTGTTGGATTGTGAGA+AGG 0.610775 5:+82497361 MsG0580028566.01.T01:CDS
AGGAAAAGAGAGAAACGAGA+AGG 0.619384 5:+82497448 MsG0580028566.01.T01:CDS
CATGTTTAAAGAAATTCCGC+CGG 0.629478 5:+82495446 MsG0580028566.01.T01:CDS
TCATTGGTTGATGTTGGAGG+AGG 0.629765 5:+82495300 MsG0580028566.01.T01:CDS
TGCAATATCAAAGAAAGGGA+AGG 0.634249 5:+82497325 MsG0580028566.01.T01:CDS
GGCACCATACGTGCACGCAA+TGG 0.634959 5:+82495059 MsG0580028566.01.T01:CDS
AATATCAAAGAAAGGGAAGG+TGG 0.637517 5:+82497328 MsG0580028566.01.T01:CDS
CTTGCTGGTTGAGAAGTGCA+TGG 0.643007 5:+82495257 MsG0580028566.01.T01:CDS
GTGTTGGATTGTGAGAAGGG+AGG 0.650456 5:+82497365 MsG0580028566.01.T01:CDS
GCAAGCCATCAACAACGTGT+GGG 0.655823 5:-82495389 None:intergenic
GACACATGGGATGTCAACAT+GGG 0.660267 5:+82495158 MsG0580028566.01.T01:CDS
TTGATGTTGGAGGAGGAACA+GGG 0.660754 5:+82495307 MsG0580028566.01.T01:CDS
TTGCTGGTTGAGAAGTGCAT+GGG 0.664757 5:+82495258 MsG0580028566.01.T01:CDS
GCACTTCTCAACCAGCAAGT+CGG 0.667814 5:-82495253 None:intergenic
GAAGGTGGTTATCATAGACA+TGG 0.675789 5:+82497343 MsG0580028566.01.T01:CDS
TAAGGACAATCCAATGAGTG+TGG 0.680404 5:+82495038 MsG0580028566.01.T01:CDS
ACACATTTGAGACGACACAT+GGG 0.693139 5:+82495145 MsG0580028566.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGATTAAAAAAATTGT+AGG - Chr5:82495672-82495691 None:intergenic 10.0%
!! AAATTAAAACAAATTAAGTA+TGG - Chr5:82495521-82495540 None:intergenic 10.0%
!! ACAAAAAAATCATTTAAAAA+TGG - Chr5:82496209-82496228 None:intergenic 10.0%
!!! AATCAAATAATTATTTCTTA+GGG + Chr5:82496173-82496192 MsG0580028566.01.T01:intron 10.0%
!! AGAGAATAATATATAGTAAA+CGG - Chr5:82495646-82495665 None:intergenic 15.0%
!! GTTAAATAAGTTGTTAATTT+CGG - Chr5:82495202-82495221 None:intergenic 15.0%
!! TATATGGAAAATTAAAATGT+AGG - Chr5:82497097-82497116 None:intergenic 15.0%
!!! AAAGTGCTATTTTAAAAATT+TGG - Chr5:82496469-82496488 None:intergenic 15.0%
!!! AAGTGTTATTTTGATAATTT+AGG + Chr5:82496731-82496750 MsG0580028566.01.T01:intron 15.0%
!!! ACATTTTAATTTTCCATATA+TGG + Chr5:82497097-82497116 MsG0580028566.01.T01:intron 15.0%
!!! ATTTTTTCTAAAATTACGAA+CGG - Chr5:82495559-82495578 None:intergenic 15.0%
!!! CAATCAAATAATTATTTCTT+AGG + Chr5:82496172-82496191 MsG0580028566.01.T01:intron 15.0%
!!! TGGATAGATATTATATTTTA+TGG - Chr5:82497560-82497579 None:intergenic 15.0%
!!! TTAATAATTTTACACAACTT+GGG - Chr5:82496957-82496976 None:intergenic 15.0%
!!! TTCTCTAAAAAAAAAGTTAT+AGG + Chr5:82494964-82494983 MsG0580028566.01.T01:CDS 15.0%
!!! TTGTTTTAATTTGCTATATT+TGG + Chr5:82495529-82495548 MsG0580028566.01.T01:intron 15.0%
!!! TTTAATAATTTTACACAACT+TGG - Chr5:82496958-82496977 None:intergenic 15.0%
!! AAGTTTAACCATATTTGAAA+AGG - Chr5:82496700-82496719 None:intergenic 20.0%
!! AGGTAAAAATGTTGAAATAT+TGG + Chr5:82495487-82495506 MsG0580028566.01.T01:intron 20.0%
!! CTAAAAGATAACTATTGTAT+TGG - Chr5:82496393-82496412 None:intergenic 20.0%
!! GGTATAATTGAAAAAAGATT+AGG - Chr5:82496811-82496830 None:intergenic 20.0%
!!! AAAGTATTCTTATCAAGTAT+TGG - Chr5:82496886-82496905 None:intergenic 20.0%
!!! AATTTCTGATCAACTTTTTT+CGG - Chr5:82496140-82496159 None:intergenic 20.0%
!!! ATGATTTTTTTGTTGAAATG+AGG + Chr5:82496216-82496235 MsG0580028566.01.T01:intron 20.0%
!!! GAAGATTTTAAAGAATTGTA+AGG + Chr5:82497301-82497320 MsG0580028566.01.T01:CDS 20.0%
!!! TGAAAATGATCATCTATTTT+AGG - Chr5:82496366-82496385 None:intergenic 20.0%
! AAATTAAAATGTAGGAGAAG+TGG - Chr5:82497089-82497108 None:intergenic 25.0%
! AAATTTGGTACTAAAATCGA+CGG - Chr5:82496454-82496473 None:intergenic 25.0%
! ACGATTATTAGTACCATATA+TGG - Chr5:82497113-82497132 None:intergenic 25.0%
! GACTTATAGTTAAATAGAGA+GGG + Chr5:82495794-82495813 MsG0580028566.01.T01:intron 25.0%
! TCAAAAGAAGATGAAGTATA+AGG - Chr5:82497149-82497168 None:intergenic 25.0%
! TCATTCATTATGAGACAAAA+CGG + Chr5:82497009-82497028 MsG0580028566.01.T01:intron 25.0%
! TTCAAATATGGTTAAACTTG+AGG + Chr5:82496701-82496720 MsG0580028566.01.T01:intron 25.0%
! TTTAACCATATTTGAAAAGG+CGG - Chr5:82496697-82496716 None:intergenic 25.0%
!! ATGAAATTGATTTCTTTAGC+TGG + Chr5:82497476-82497495 MsG0580028566.01.T01:CDS 25.0%
!! TTTTCATATGTCATCAATGA+GGG + Chr5:82495689-82495708 MsG0580028566.01.T01:intron 25.0%
!! TTTTTCATATGTCATCAATG+AGG + Chr5:82495688-82495707 MsG0580028566.01.T01:intron 25.0%
!!! AGTTCACTGTCATTTTTTAT+AGG + Chr5:82496249-82496268 MsG0580028566.01.T01:intron 25.0%
AAATATGGTTAAACTTGAGG+AGG + Chr5:82496704-82496723 MsG0580028566.01.T01:intron 30.0%
AACTACACATTTAAGTGACA+TGG - Chr5:82494806-82494825 None:intergenic 30.0%
AACTCTTAATGAAATTGCAG+TGG + Chr5:82497246-82497265 MsG0580028566.01.T01:intron 30.0%
AAGGATGCAATATCAAAGAA+AGG + Chr5:82497320-82497339 MsG0580028566.01.T01:CDS 30.0%
AAGTAAGAGTTTAGATGCAT+CGG - Chr5:82495019-82495038 None:intergenic 30.0%
AGGATGCAATATCAAAGAAA+GGG + Chr5:82497321-82497340 MsG0580028566.01.T01:CDS 30.0%
AGTTGATCAGAAATTCATAG+AGG + Chr5:82496145-82496164 MsG0580028566.01.T01:intron 30.0%
ATAGACCTTAAGCCTAAAAT+TGG - Chr5:82497524-82497543 None:intergenic 30.0%
ATCCTTCAACTTACATAAAC+TGG + Chr5:82496623-82496642 MsG0580028566.01.T01:intron 30.0%
ATTCAAGTAGCAAGTAAGTA+TGG + Chr5:82494696-82494715 MsG0580028566.01.T01:exon 30.0%
CAACTTATTTAACGAGTCAA+TGG + Chr5:82495209-82495228 MsG0580028566.01.T01:CDS 30.0%
CGACTTATAGTTAAATAGAG+AGG + Chr5:82495793-82495812 MsG0580028566.01.T01:intron 30.0%
GAAATCGTATAACTGAAAGA+GGG - Chr5:82496935-82496954 None:intergenic 30.0%
GTGATGCATTGAAAATTAGA+TGG - Chr5:82496832-82496851 None:intergenic 30.0%
GTGGTGAATTTCATATAGAA+GGG + Chr5:82496281-82496300 MsG0580028566.01.T01:intron 30.0%
TAAACATGTGATTCCATACA+TGG - Chr5:82494771-82494790 None:intergenic 30.0%
TAAATGAATCACTCGTTAGA+AGG - Chr5:82496322-82496341 None:intergenic 30.0%
TGAAATTTCATTTACACGTC+AGG - Chr5:82495895-82495914 None:intergenic 30.0%
TGATAACACATCCTTAAACA+GGG - Chr5:82496111-82496130 None:intergenic 30.0%
TGCATCTAAACTCTTACTTA+AGG + Chr5:82495020-82495039 MsG0580028566.01.T01:CDS 30.0%
TGGTGAATTTCATATAGAAG+GGG + Chr5:82496282-82496301 MsG0580028566.01.T01:intron 30.0%
TTGCAAAAAATGGCTAAGTT+GGG - Chr5:82496496-82496515 None:intergenic 30.0%
TTTGCAAAAAATGGCTAAGT+TGG - Chr5:82496497-82496516 None:intergenic 30.0%
! CTTAAATGTGTAGTTGACTT+AGG + Chr5:82494811-82494830 MsG0580028566.01.T01:CDS 30.0%
! CTTCTTTGATATGTTGATGA+TGG + Chr5:82497415-82497434 MsG0580028566.01.T01:CDS 30.0%
! TACAAGATCACTCCAATTTT+AGG + Chr5:82497509-82497528 MsG0580028566.01.T01:CDS 30.0%
!! TTGACTTGGTCAAAATCAAA+GGG - Chr5:82496528-82496547 None:intergenic 30.0%
!!! CATTTTTTATAGGTGAGTGT+TGG + Chr5:82496259-82496278 MsG0580028566.01.T01:intron 30.0%
!!! GAAGAAATAAGTTTTGAGAG+TGG - Chr5:82494874-82494893 None:intergenic 30.0%
!!! TTAGCCATTTTTTGCAAAAG+TGG + Chr5:82496499-82496518 MsG0580028566.01.T01:intron 30.0%
AATATCAAAGAAAGGGAAGG+TGG + Chr5:82497328-82497347 MsG0580028566.01.T01:CDS 35.0%
AGATATTGTGTGCGATGAAT+TGG - Chr5:82495925-82495944 None:intergenic 35.0%
AGTGAGAAGCTAAGATATGT+TGG + Chr5:82495417-82495436 MsG0580028566.01.T01:CDS 35.0%
ATATTGTGTGCGATGAATTG+GGG - Chr5:82495923-82495942 None:intergenic 35.0%
ATCCAGTTATGATGAATCCA+TGG + Chr5:82495085-82495104 MsG0580028566.01.T01:CDS 35.0%
CATGTTTAAAGAAATTCCGC+CGG + Chr5:82495446-82495465 MsG0580028566.01.T01:CDS 35.0%
CCAGATATCATACACAACTA+TGG + Chr5:82494838-82494857 MsG0580028566.01.T01:CDS 35.0%
CCATAGTTGTGTATGATATC+TGG - Chr5:82494841-82494860 None:intergenic 35.0%
GATAACACATCCTTAAACAG+GGG - Chr5:82496110-82496129 None:intergenic 35.0%
GATATTGTGTGCGATGAATT+GGG - Chr5:82495924-82495943 None:intergenic 35.0%
GGAAATCGTATAACTGAAAG+AGG - Chr5:82496936-82496955 None:intergenic 35.0%
GGTGGTGAATTTCATATAGA+AGG + Chr5:82496280-82496299 MsG0580028566.01.T01:intron 35.0%
GTGATAACACATCCTTAAAC+AGG - Chr5:82496112-82496131 None:intergenic 35.0%
GTTCAATGAAGTTGAGTCAT+TGG + Chr5:82495284-82495303 MsG0580028566.01.T01:CDS 35.0%
GTTGACCTAGTCAAAATCAA+GGG + Chr5:82496668-82496687 MsG0580028566.01.T01:intron 35.0%
TATAACTGAAAGAGGGACTT+GGG - Chr5:82496928-82496947 None:intergenic 35.0%
TGCAAAAAATGGCTAAGTTG+GGG - Chr5:82496495-82496514 None:intergenic 35.0%
TGCAATATCAAAGAAAGGGA+AGG + Chr5:82497325-82497344 MsG0580028566.01.T01:CDS 35.0%
TTCAACAAAATTGCATCTGC+CGG - Chr5:82495468-82495487 None:intergenic 35.0%
TTGACCTAGTCAAAATCAAG+GGG + Chr5:82496669-82496688 MsG0580028566.01.T01:intron 35.0%
! AAGCAACCTGCTCTATATTT+TGG - Chr5:82494726-82494745 None:intergenic 35.0%
! AGCAACCTGCTCTATATTTT+GGG - Chr5:82494725-82494744 None:intergenic 35.0%
! GAACGACGTTGATGAAAATT+GGG - Chr5:82496004-82496023 None:intergenic 35.0%
! GGTTGTTATTGAAAGTTGGA+AGG - Chr5:82496433-82496452 None:intergenic 35.0%
! TCACTCCAATTTTAGGCTTA+AGG + Chr5:82497516-82497535 MsG0580028566.01.T01:CDS 35.0%
! TCTCTCTTTTCCTGTTAGTA+GGG - Chr5:82497441-82497460 None:intergenic 35.0%
! TTCTCTCTTTTCCTGTTAGT+AGG - Chr5:82497442-82497461 None:intergenic 35.0%
!! GACACCACTTTTGCAAAAAA+TGG - Chr5:82496506-82496525 None:intergenic 35.0%
!! GTTGACTTGGTCAAAATCAA+AGG - Chr5:82496529-82496548 None:intergenic 35.0%
!!! AATAAGTTTTGAGAGTGGCA+TGG - Chr5:82494869-82494888 None:intergenic 35.0%
!!! ATAAGTTTTGAGAGTGGCAT+GGG - Chr5:82494868-82494887 None:intergenic 35.0%
!!! TTTTTATAGGTGAGTGTTGG+TGG + Chr5:82496262-82496281 MsG0580028566.01.T01:intron 35.0%
AACACAATGCACTCCATTTG+TGG - Chr5:82495363-82495382 None:intergenic 40.0%
AAGAGAGAAACGAGAAGGAA+TGG + Chr5:82497453-82497472 MsG0580028566.01.T01:CDS 40.0%
ACACAATGCACTCCATTTGT+GGG - Chr5:82495362-82495381 None:intergenic 40.0%
ACCTCCTAAGTTTCACACAA+CGG - Chr5:82496768-82496787 None:intergenic 40.0%
AGGAAAAGAGAGAAACGAGA+AGG + Chr5:82497448-82497467 MsG0580028566.01.T01:CDS 40.0%
ATCCACCCTTCAAAAAACCA+TGG + Chr5:82494901-82494920 MsG0580028566.01.T01:CDS 40.0%
CAAGTAGAGAATTGATGCCA+TGG - Chr5:82495105-82495124 None:intergenic 40.0%
CATGGCATCAATTCTCTACT+TGG + Chr5:82495103-82495122 MsG0580028566.01.T01:CDS 40.0%
CGTTGACCTAGTCAAAATCA+AGG + Chr5:82496667-82496686 MsG0580028566.01.T01:intron 40.0%
CTCCATTTGTGGGAATGATT+TGG - Chr5:82495352-82495371 None:intergenic 40.0%
GAAGGTGGTTATCATAGACA+TGG + Chr5:82497343-82497362 MsG0580028566.01.T01:CDS 40.0%
GAGAAGCTAAGATATGTTGG+TGG + Chr5:82495420-82495439 MsG0580028566.01.T01:CDS 40.0%
GGAATCCCAAAATATAGAGC+AGG + Chr5:82494717-82494736 MsG0580028566.01.T01:CDS 40.0%
GGTTATCATAGACATGGTGT+TGG + Chr5:82497349-82497368 MsG0580028566.01.T01:CDS 40.0%
GTATAACTGAAAGAGGGACT+TGG - Chr5:82496929-82496948 None:intergenic 40.0%
TAAGGACAATCCAATGAGTG+TGG + Chr5:82495038-82495057 MsG0580028566.01.T01:CDS 40.0%
TACGGTAGGAGCAAAAATGT+AGG - Chr5:82497064-82497083 None:intergenic 40.0%
TCAAAGCTCAAAGCCATGTA+TGG + Chr5:82494755-82494774 MsG0580028566.01.T01:CDS 40.0%
TGCCAAATCATTCCCACAAA+TGG + Chr5:82495347-82495366 MsG0580028566.01.T01:CDS 40.0%
TGCCATGGATTCATCATAAC+TGG - Chr5:82495090-82495109 None:intergenic 40.0%
! AACACATTTGAGACGACACA+TGG + Chr5:82495144-82495163 MsG0580028566.01.T01:CDS 40.0%
! ACACATTTGAGACGACACAT+GGG + Chr5:82495145-82495164 MsG0580028566.01.T01:CDS 40.0%
! ATGGTGTTGGATTGTGAGAA+GGG + Chr5:82497362-82497381 MsG0580028566.01.T01:CDS 40.0%
! CGACGGTTGTTATTGAAAGT+TGG - Chr5:82496437-82496456 None:intergenic 40.0%
! CGCCAGTTTATGTAAGTTGA+AGG - Chr5:82496628-82496647 None:intergenic 40.0%
! GCAAAAAATGGCTAAGTTGG+GGG - Chr5:82496494-82496513 None:intergenic 40.0%
! GGAACGACGTTGATGAAAAT+TGG - Chr5:82496005-82496024 None:intergenic 40.0%
! GGCAGATGCAATTTTGTTGA+AGG + Chr5:82495467-82495486 MsG0580028566.01.T01:CDS 40.0%
! GTTGAGTCATTGGTTGATGT+TGG + Chr5:82495294-82495313 MsG0580028566.01.T01:CDS 40.0%
!! AATGTAGGAGAAGTGGACTA+CGG - Chr5:82497082-82497101 None:intergenic 40.0%
!! GCTTTGAGCTTTGAGCAAAT+TGG - Chr5:82494749-82494768 None:intergenic 40.0%
!!! GATTTTGACTAGGTCAACGT+CGG - Chr5:82496666-82496685 None:intergenic 40.0%
!!! GGATGCCATGGTTTTTTGAA+GGG - Chr5:82494909-82494928 None:intergenic 40.0%
!!! TGGATGCCATGGTTTTTTGA+AGG - Chr5:82494910-82494929 None:intergenic 40.0%
AACAAAATTGCATCTGCCGG+CGG - Chr5:82495465-82495484 None:intergenic 45.0%
ACGACAAAGGAACGATGTGA+CGG - Chr5:82496078-82496097 None:intergenic 45.0%
ACGTCAGGTACACGTAATTC+GGG - Chr5:82495880-82495899 None:intergenic 45.0%
ATCGCCGTTGTGTGAAACTT+AGG + Chr5:82496761-82496780 MsG0580028566.01.T01:intron 45.0%
ATGCGAATCATGACTCACTC+TGG + Chr5:82494937-82494956 MsG0580028566.01.T01:CDS 45.0%
ATTGGGAGAAACACGATGAG+TGG - Chr5:82495987-82496006 None:intergenic 45.0%
CACGTCAGGTACACGTAATT+CGG - Chr5:82495881-82495900 None:intergenic 45.0%
CGATGACAAAGTCACAGCAA+AGG - Chr5:82496026-82496045 None:intergenic 45.0%
GACACATGGGATGTCAACAT+GGG + Chr5:82495158-82495177 MsG0580028566.01.T01:CDS 45.0%
TACTTCGTTGCCCCTGTTTA+AGG + Chr5:82496097-82496116 MsG0580028566.01.T01:intron 45.0%
TCATGATTCGCATCAAGCGA+TGG - Chr5:82494930-82494949 None:intergenic 45.0%
TGCAGTGGATATTGCATGAC+TGG + Chr5:82497261-82497280 MsG0580028566.01.T01:intron 45.0%
TGTTGGATTGTGAGAAGGGA+GGG + Chr5:82497366-82497385 MsG0580028566.01.T01:CDS 45.0%
TTGATGTTGGAGGAGGAACA+GGG + Chr5:82495307-82495326 MsG0580028566.01.T01:CDS 45.0%
! ACCTCTCGGGACACTTTTAT+CGG - Chr5:82495856-82495875 None:intergenic 45.0%
! CATGGTGTTGGATTGTGAGA+AGG + Chr5:82497361-82497380 MsG0580028566.01.T01:CDS 45.0%
! GAGTCATTGGTTGATGTTGG+AGG + Chr5:82495297-82495316 MsG0580028566.01.T01:CDS 45.0%
! TCATTGGTTGATGTTGGAGG+AGG + Chr5:82495300-82495319 MsG0580028566.01.T01:CDS 45.0%
! TCGATTAGTATCCGACTTGC+TGG + Chr5:82495242-82495261 MsG0580028566.01.T01:CDS 45.0%
! TTGCTGGTTGAGAAGTGCAT+GGG + Chr5:82495258-82495277 MsG0580028566.01.T01:CDS 45.0%
!! GGGTACCGCCTTTTCAAATA+TGG + Chr5:82496689-82496708 MsG0580028566.01.T01:intron 45.0%
!! TTGATGATGGCCCTACTAAC+AGG + Chr5:82497428-82497447 MsG0580028566.01.T01:CDS 45.0%
!!! GGTACCCCTTGATTTTGACT+AGG - Chr5:82496676-82496695 None:intergenic 45.0%
!!! TGCCATGGTTTTTTGAAGGG+TGG - Chr5:82494906-82494925 None:intergenic 45.0%
ACGTAATTCGGGAACCTCTC+GGG - Chr5:82495869-82495888 None:intergenic 50.0%
CACGTAATTCGGGAACCTCT+CGG - Chr5:82495870-82495889 None:intergenic 50.0%
CGACACATGGGATGTCAACA+TGG + Chr5:82495157-82495176 MsG0580028566.01.T01:CDS 50.0%
GATCTCCCACACGTTGTTGA+TGG + Chr5:82495384-82495403 MsG0580028566.01.T01:CDS 50.0%
GCAAGCCATCAACAACGTGT+GGG - Chr5:82495392-82495411 None:intergenic 50.0%
GCACTTCTCAACCAGCAAGT+CGG - Chr5:82495256-82495275 None:intergenic 50.0%
GCCGTTGTGTGAAACTTAGG+AGG + Chr5:82496764-82496783 MsG0580028566.01.T01:intron 50.0%
GGAGAAACACGATGAGTGGT+GGG - Chr5:82495983-82496002 None:intergenic 50.0%
GTGTTGGATTGTGAGAAGGG+AGG + Chr5:82497365-82497384 MsG0580028566.01.T01:CDS 50.0%
GTTGATGTTGGAGGAGGAAC+AGG + Chr5:82495306-82495325 MsG0580028566.01.T01:CDS 50.0%
TGACCAAGTCAACGCATACG+TGG + Chr5:82496536-82496555 MsG0580028566.01.T01:intron 50.0%
TGCAAGCCATCAACAACGTG+TGG - Chr5:82495393-82495412 None:intergenic 50.0%
TTGACCATTGCGTGCACGTA+TGG - Chr5:82495066-82495085 None:intergenic 50.0%
! ACTAGGTCAACGTCGGTGTT+GGG - Chr5:82496659-82496678 None:intergenic 50.0%
! CCTCTCGGGACACTTTTATC+GGG - Chr5:82495855-82495874 None:intergenic 50.0%
! CTTGCTGGTTGAGAAGTGCA+TGG + Chr5:82495257-82495276 MsG0580028566.01.T01:CDS 50.0%
!! ACGTATGGTGCCACACTCAT+TGG - Chr5:82495051-82495070 None:intergenic 50.0%
!! CACGTTGTTGATGGCTTGCA+AGG + Chr5:82495393-82495412 MsG0580028566.01.T01:CDS 50.0%
!! TAGGAGAAGTGGACTACGGT+AGG - Chr5:82497078-82497097 None:intergenic 50.0%
!! TGTCCACGTATGCGTTGACT+TGG - Chr5:82496542-82496561 None:intergenic 50.0%
AGGAACGATGTGACGGCGAA+AGG - Chr5:82496051-82496070 None:intergenic 55.0%
CGGCGAAAGGAACGATGTGA+CGG - Chr5:82496058-82496077 None:intergenic 55.0%
GACTAGGTCAACGTCGGTGT+TGG - Chr5:82496660-82496679 None:intergenic 55.0%
GCATCAAGCGATGGATGCCA+TGG - Chr5:82494921-82494940 None:intergenic 55.0%
GGGAGAAACACGATGAGTGG+TGG - Chr5:82495984-82496003 None:intergenic 55.0%
GGGGCAACGAAGTACGACAA+AGG - Chr5:82496091-82496110 None:intergenic 55.0%
TGGAGGAGGAACAGGGACTA+TGG + Chr5:82495314-82495333 MsG0580028566.01.T01:CDS 55.0%
TGGTGGTCGCTGGATTCGTA+GGG - Chr5:82495958-82495977 None:intergenic 55.0%
!! AACACGATGAGTGGTGGGTG+TGG - Chr5:82495978-82495997 None:intergenic 55.0%
!! CCCGATAAAAGTGTCCCGAG+AGG + Chr5:82495852-82495871 MsG0580028566.01.T01:intron 55.0%
!! CTAGGTCAACGTCGGTGTTG+GGG - Chr5:82496658-82496677 None:intergenic 55.0%
GGCACCATACGTGCACGCAA+TGG + Chr5:82495059-82495078 MsG0580028566.01.T01:CDS 60.0%
GTGGTGGTCGCTGGATTCGT+AGG - Chr5:82495959-82495978 None:intergenic 60.0%
!! ACGATGAGTGGTGGGTGTGG+TGG - Chr5:82495975-82495994 None:intergenic 60.0%
!! GTGGTGGGTGTGGTGGTCGC+TGG - Chr5:82495968-82495987 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 82494685 82497592 82494685 ID=MsG0580028566.01;Name=MsG0580028566.01
Chr5 mRNA 82494685 82497592 82494685 ID=MsG0580028566.01.T01;Parent=MsG0580028566.01;Name=MsG0580028566.01.T01;_AED=0.27;_eAED=0.27;_QI=30|1|1|1|1|1|2|30|356
Chr5 exon 82494685 82495488 82494685 ID=MsG0580028566.01.T01:exon:21202;Parent=MsG0580028566.01.T01
Chr5 exon 82497266 82497592 82497266 ID=MsG0580028566.01.T01:exon:21203;Parent=MsG0580028566.01.T01
Chr5 five_prime_UTR 82494685 82494714 82494685 ID=MsG0580028566.01.T01:five_prime_utr;Parent=MsG0580028566.01.T01
Chr5 CDS 82494715 82495488 82494715 ID=MsG0580028566.01.T01:cds;Parent=MsG0580028566.01.T01
Chr5 CDS 82497266 82497562 82497266 ID=MsG0580028566.01.T01:cds;Parent=MsG0580028566.01.T01
Chr5 three_prime_UTR 82497563 82497592 82497563 ID=MsG0580028566.01.T01:three_prime_utr;Parent=MsG0580028566.01.T01
Gene Sequence

>MsG0580028566.01.T01

ATGGAATCCCAAAATATAGAGCAGGTTGCTTCCAATTTGCTCAAAGCTCAAAGCCATGTATGGAATCACATGTTTAACTTTATAAATTCCATGTCACTTAAATGTGTAGTTGACTTAGGCATACCAGATATCATACACAACTATGGCAAACCCATGCCACTCTCAAAACTTATTTCTTCATTATCAATCCACCCTTCAAAAAACCATGGCATCCATCGCTTGATGCGAATCATGACTCACTCTGGCTTCTTCTCTAAAAAAAAAGTTATAGGAAATGAGCTAGAAATTGAGTATGTGTTAACCGATGCATCTAAACTCTTACTTAAGGACAATCCAATGAGTGTGGCACCATACGTGCACGCAATGGTCAATCCAGTTATGATGAATCCATGGCATCAATTCTCTACTTGGTTAAAAAATGACGATACTAACACATTTGAGACGACACATGGGATGTCAACATGGGAGTATGCTGCACGTGACTCCGAAATTAACAACTTATTTAACGAGTCAATGGCAAATGATACTCGATTAGTATCCGACTTGCTGGTTGAGAAGTGCATGGGAGTGTTCAATGAAGTTGAGTCATTGGTTGATGTTGGAGGAGGAACAGGGACTATGGCAAAGACTCTTGCCAAATCATTCCCACAAATGGAGTGCATTGTGTTTGATCTCCCACACGTTGTTGATGGCTTGCAAGGAAGTGAGAAGCTAAGATATGTTGGTGGAGACATGTTTAAAGAAATTCCGCCGGCAGATGCAATTTTGTTGAAGTGGATATTGCATGACTGGAACGACGAGCAATGTCTGAAGATTTTAAAGAATTGTAAGGATGCAATATCAAAGAAAGGGAAGGTGGTTATCATAGACATGGTGTTGGATTGTGAGAAGGGAGGGAATAATGAATCAGTTGAAACACAACTCTTCTTTGATATGTTGATGATGGCCCTACTAACAGGAAAAGAGAGAAACGAGAAGGAATGGATGAAATTGATTTCTTTAGCTGGTTTTAGTGACTACAAGATCACTCCAATTTTAGGCTTAAGGTCTATGATTGAGATCTATCCATAA
Protein sequence

>MsG0580028566.01.T01

MESQNIEQVASNLLKAQSHVWNHMFNFINSMSLKCVVDLGIPDIIHNYGKPMPLSKLISSLSIHPSKNHGIHRLMRIMTHSGFFSKKKVIGNELEIEYVLTDASKLLLKDNPMSVAPYVHAMVNPVMMNPWHQFSTWLKNDDTNTFETTHGMSTWEYAARDSEINNLFNESMANDTRLVSDLLVEKCMGVFNEVESLVDVGGGTGTMAKTLAKSFPQMECIVFDLPHVVDGLQGSEKLRYVGGDMFKEIPPADAILLKWILHDWNDEQCLKILKNCKDAISKKGKVVIIDMVLDCEKGGNNESVETQLFFDMLMMALLTGKERNEKEWMKLISLAGFSDYKITPILGLRSMIEIYP*