Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029186.01.T01 | XP_003616621.1 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 1.66E-100 | 294 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029186.01.T01 | Q9ST43 | 68.794 | 141 | 42 | 1 | 4 | 142 | 3 | 143 | 1.87E-69 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029186.01.T01 | G7KGZ7 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 7.92e-101 | 294 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003309.01 | MsG0580029186.01 | 0.806835 | 6.783537e-50 | 3.719615e-47 |
| MsG0480021776.01 | MsG0580029186.01 | 0.800051 | 1.727623e-48 | 7.965557e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580029186.01 | MsG0380016379.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029186.01.T01 | MTR_5g082470 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 2.01e-104 | 294 |
| MsG0580029186.01.T01 | MTR_3g463520 | 70.922 | 141 | 39 | 1 | 4 | 142 | 3 | 143 | 1.02e-73 | 217 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029186.01.T01 | AT5G05710 | 79.433 | 141 | 28 | 1 | 3 | 142 | 2 | 142 | 3.70e-80 | 233 |
| MsG0580029186.01.T01 | AT2G29700 | 68.794 | 141 | 42 | 1 | 4 | 142 | 3 | 143 | 1.91e-70 | 209 |
Find 29 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTACTTGTCTTACTGTTAA+AGG | 0.201576 | 5:-93395844 | MsG0580029186.01.T01:CDS |
| GTTAACCAGCCGGTTCGTTC+TGG | 0.208272 | 5:+93395991 | None:intergenic |
| ACTACTCCGGCGTCGAATTC+TGG | 0.239180 | 5:-93396020 | MsG0580029186.01.T01:CDS |
| TGAAACAAGGGAAGCTCTTC+TGG | 0.240314 | 5:-93395921 | MsG0580029186.01.T01:CDS |
| GAGGATTGGATTAACTCTAT+TGG | 0.299879 | 5:-93395730 | MsG0580029186.01.T01:CDS |
| CCGGAATGGCAGCGAGTCTT+TGG | 0.316895 | 5:-93396077 | None:intergenic |
| TTAACCAGCCGGTTCGTTCT+GGG | 0.377723 | 5:+93395992 | None:intergenic |
| GAAATTGTTGATTATGATAA+CGG | 0.378590 | 5:-93395667 | MsG0580029186.01.T01:CDS |
| TTCTAGATCTGTTACTGATT+CGG | 0.390424 | 5:-93395689 | MsG0580029186.01.T01:CDS |
| ACAGTAAGACAAGTAGCAAC+AGG | 0.406909 | 5:+93395850 | None:intergenic |
| AGTCTTTGGCGCTACACAAC+CGG | 0.467859 | 5:-93396063 | MsG0580029186.01.T01:CDS |
| GACCGGGTTTGTGGTTGAGC+CGG | 0.470641 | 5:+93396044 | None:intergenic |
| TGATTCTGATAAGGAGAAAG+AGG | 0.505978 | 5:-93395749 | MsG0580029186.01.T01:CDS |
| CTGATAAGGAGAAAGAGGAT+TGG | 0.515649 | 5:-93395744 | MsG0580029186.01.T01:CDS |
| TGGTCGAACCCAGAACGAAC+CGG | 0.518095 | 5:-93396000 | MsG0580029186.01.T01:CDS |
| GGAGTAGTCGACCGGGTTTG+TGG | 0.532363 | 5:+93396035 | None:intergenic |
| ACAAACCCGGTCGACTACTC+CGG | 0.537859 | 5:-93396033 | MsG0580029186.01.T01:CDS |
| TCGACGCCGGAGTAGTCGAC+CGG | 0.566459 | 5:+93396027 | None:intergenic |
| AACCGGCTCAACCACAAACC+CGG | 0.568891 | 5:-93396046 | MsG0580029186.01.T01:CDS |
| GTGGAAAGTTCAAACGAGTA+GGG | 0.571418 | 5:+93395799 | None:intergenic |
| AGTGGAAAGTTCAAACGAGT+AGG | 0.587441 | 5:+93395798 | None:intergenic |
| ATACATCGTATCAGCGCGAG+TGG | 0.592591 | 5:+93395780 | None:intergenic |
| CGAACCCAGAACGAACCGGC+TGG | 0.620445 | 5:-93395996 | MsG0580029186.01.T01:CDS |
| CGACGCCGGAGTAGTCGACC+GGG | 0.622012 | 5:+93396028 | None:intergenic |
| ACCGGCTGGTTAACAAAACA+AGG | 0.628016 | 5:-93395982 | MsG0580029186.01.T01:CDS |
| CAGTAAGACAAGTAGCAACA+GGG | 0.658308 | 5:+93395851 | None:intergenic |
| GTTCGACCAGAATTCGACGC+CGG | 0.725090 | 5:+93396014 | None:intergenic |
| ACACGCGCTTCTATACCGCG+TGG | 0.799874 | 5:-93395880 | MsG0580029186.01.T01:CDS |
| GCAACAGGGATAACACCACG+CGG | 0.840792 | 5:+93395865 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTGTTGATTATGATAA+CGG | - | Chr5:93396065-93396084 | MsG0580029186.01.T01:CDS | 20.0% |
| !!! | TTTTATTGCTGATTCTGATA+AGG | - | Chr5:93395974-93395993 | MsG0580029186.01.T01:CDS | 25.0% |
| AAGGCGAATACATAAAAACA+TGG | - | Chr5:93395769-93395788 | MsG0580029186.01.T01:CDS | 30.0% | |
| ! | TTCTAGATCTGTTACTGATT+CGG | - | Chr5:93396043-93396062 | MsG0580029186.01.T01:CDS | 30.0% |
| GAGGATTGGATTAACTCTAT+TGG | - | Chr5:93396002-93396021 | MsG0580029186.01.T01:CDS | 35.0% | |
| GCTACTTGTCTTACTGTTAA+AGG | - | Chr5:93395888-93395907 | MsG0580029186.01.T01:CDS | 35.0% | |
| TGATTCTGATAAGGAGAAAG+AGG | - | Chr5:93395983-93396002 | MsG0580029186.01.T01:CDS | 35.0% | |
| !!! | GTTGGTTCGTTTTGAAACAA+GGG | - | Chr5:93395799-93395818 | MsG0580029186.01.T01:CDS | 35.0% |
| ACAGTAAGACAAGTAGCAAC+AGG | + | Chr5:93395885-93395904 | None:intergenic | 40.0% | |
| AGTGGAAAGTTCAAACGAGT+AGG | + | Chr5:93395937-93395956 | None:intergenic | 40.0% | |
| CAGTAAGACAAGTAGCAACA+GGG | + | Chr5:93395884-93395903 | None:intergenic | 40.0% | |
| CTGATAAGGAGAAAGAGGAT+TGG | - | Chr5:93395988-93396007 | MsG0580029186.01.T01:CDS | 40.0% | |
| GTGGAAAGTTCAAACGAGTA+GGG | + | Chr5:93395936-93395955 | None:intergenic | 40.0% | |
| TAAAAACATGGCGAAGACGT+TGG | - | Chr5:93395781-93395800 | MsG0580029186.01.T01:CDS | 40.0% | |
| !!! | CGTTGGTTCGTTTTGAAACA+AGG | - | Chr5:93395798-93395817 | MsG0580029186.01.T01:CDS | 40.0% |
| TGAAACAAGGGAAGCTCTTC+TGG | - | Chr5:93395811-93395830 | MsG0580029186.01.T01:CDS | 45.0% | |
| ! | ACCGGCTGGTTAACAAAACA+AGG | - | Chr5:93395750-93395769 | MsG0580029186.01.T01:CDS | 45.0% |
| !!! | GCCTTGTTTTGTTAACCAGC+CGG | + | Chr5:93395754-93395773 | None:intergenic | 45.0% |
| AGTCTTTGGCGCTACACAAC+CGG | - | Chr5:93395669-93395688 | MsG0580029186.01.T01:CDS | 50.0% | |
| ATACATCGTATCAGCGCGAG+TGG | + | Chr5:93395955-93395974 | None:intergenic | 50.0% | |
| !! | TTAACCAGCCGGTTCGTTCT+GGG | + | Chr5:93395743-93395762 | None:intergenic | 50.0% |
| ACAAACCCGGTCGACTACTC+CGG | - | Chr5:93395699-93395718 | MsG0580029186.01.T01:CDS | 55.0% | |
| GCAACAGGGATAACACCACG+CGG | + | Chr5:93395870-93395889 | None:intergenic | 55.0% | |
| GTTCGACCAGAATTCGACGC+CGG | + | Chr5:93395721-93395740 | None:intergenic | 55.0% | |
| TGGTCGAACCCAGAACGAAC+CGG | - | Chr5:93395732-93395751 | MsG0580029186.01.T01:CDS | 55.0% | |
| ! | AACCGGCTCAACCACAAACC+CGG | - | Chr5:93395686-93395705 | MsG0580029186.01.T01:CDS | 55.0% |
| ! | ACTACTCCGGCGTCGAATTC+TGG | - | Chr5:93395712-93395731 | MsG0580029186.01.T01:CDS | 55.0% |
| !! | GTTAACCAGCCGGTTCGTTC+TGG | + | Chr5:93395744-93395763 | None:intergenic | 55.0% |
| ACACGCGCTTCTATACCGCG+TGG | - | Chr5:93395852-93395871 | MsG0580029186.01.T01:CDS | 60.0% | |
| GACCGGGTTTGTGGTTGAGC+CGG | + | Chr5:93395691-93395710 | None:intergenic | 60.0% | |
| GGAGTAGTCGACCGGGTTTG+TGG | + | Chr5:93395700-93395719 | None:intergenic | 60.0% | |
| CGAACCCAGAACGAACCGGC+TGG | - | Chr5:93395736-93395755 | MsG0580029186.01.T01:CDS | 65.0% | |
| TCGACGCCGGAGTAGTCGAC+CGG | + | Chr5:93395708-93395727 | None:intergenic | 65.0% | |
| CGACGCCGGAGTAGTCGACC+GGG | + | Chr5:93395707-93395726 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 93395660 | 93396094 | 93395660 | ID=MsG0580029186.01;Name=MsG0580029186.01 |
| Chr5 | mRNA | 93395660 | 93396094 | 93395660 | ID=MsG0580029186.01.T01;Parent=MsG0580029186.01;Name=MsG0580029186.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|144 |
| Chr5 | exon | 93395660 | 93396094 | 93395660 | ID=MsG0580029186.01.T01:exon:49667;Parent=MsG0580029186.01.T01 |
| Chr5 | CDS | 93395660 | 93396094 | 93395660 | ID=MsG0580029186.01.T01:cds;Parent=MsG0580029186.01.T01 |
| Gene Sequence |
| Protein sequence |
