AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029427.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029427.01.T01 MTR_5g085980 58.042 286 21 6 1 225 1 248 6.65e-96 280
MsG0580029427.01.T01 MTR_5g085980 57.692 286 20 7 1 225 1 246 4.50e-92 270
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029427.01.T01 AT1G05060 37.931 290 77 5 1 225 1 252 1.24e-53 173

Find 48 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGATCTATCTAAAATTT+TGG 0.076463 5:-96854456 MsG0580029427.01.T01:CDS
TAACGGCTTGTTTGGATTCT+TGG 0.230694 5:+96857492 None:intergenic
AACTGTATCATCTCATTTAC+AGG 0.308953 5:+96854150 None:intergenic
GGTGGTGCTGCAATGGTAAT+TGG 0.357696 5:-96854935 MsG0580029427.01.T01:CDS
GATGCTGCTTTCAACTTCTC+CGG 0.362635 5:+96856906 None:intergenic
TCAATTTGCATTGAAAATAG+TGG 0.369899 5:-96854385 MsG0580029427.01.T01:three_prime_UTR
ATTGCCACTGCAGTTGTATT+CGG 0.384023 5:-96854956 MsG0580029427.01.T01:CDS
GCTGAAGAATGAAAATAGTA+TGG 0.393199 5:-96854282 MsG0580029427.01.T01:three_prime_UTR
TCGGGCATGCTGCATGAAAC+TGG 0.401178 5:-96855643 MsG0580029427.01.T01:intron
CACAATTTGTTGCAATAAGA+TGG 0.407176 5:-96854175 MsG0580029427.01.T01:three_prime_UTR
TACATTATGCAGCTCTAACT+TGG 0.413171 5:+96854901 None:intergenic
TATGTCAAGAAAGTGGCATT+TGG 0.416481 5:-96854510 MsG0580029427.01.T01:CDS
TTTCTAGCGGGAAGGTTAGC+AGG 0.436098 5:-96857536 MsG0580029427.01.T01:CDS
AATGGTCATCATTATAATCA+TGG 0.476138 5:-96855682 MsG0580029427.01.T01:CDS
GCCTCTGGCTTATGTTGTTG+TGG 0.484597 5:-96854328 MsG0580029427.01.T01:three_prime_UTR
TACAGCTAATGAATTGCGTC+AGG 0.486536 5:-96856952 MsG0580029427.01.T01:CDS
ATATGAATGTAGTGTGCCTC+TGG 0.489771 5:-96854343 MsG0580029427.01.T01:three_prime_UTR
ATGCTGCTTTCAACTTCTCC+GGG 0.519765 5:+96856907 None:intergenic
AGTCAGGTTGTGTGTGGTAA+TGG 0.522939 5:-96855700 MsG0580029427.01.T01:intron
TGCTGCAATGGTAATTGGTA+TGG 0.525519 5:-96854930 MsG0580029427.01.T01:CDS
CAAGAAAGTGGCATTTGGAA+AGG 0.526917 5:-96854505 MsG0580029427.01.T01:CDS
ATGGTATATGTAGTAAATAA+CGG 0.531661 5:-96854263 MsG0580029427.01.T01:three_prime_UTR
ATGGATGACCTCAAAACTAA+AGG 0.534397 5:-96854688 MsG0580029427.01.T01:CDS
GTGTCGTTCATCAACGGCGT+TGG 0.544837 5:+96857433 None:intergenic
ATTATGCAGCTCTAACTTGG+AGG 0.547168 5:+96854904 None:intergenic
AAGAAAGTGGCATTTGGAAA+GGG 0.553714 5:-96854504 MsG0580029427.01.T01:CDS
CTAATGAATTGCGTCAGGAC+CGG 0.564086 5:-96856947 MsG0580029427.01.T01:CDS
CTGAGAATATGTCAAGAAAG+TGG 0.564264 5:-96854517 MsG0580029427.01.T01:CDS
AAGTTGAAAGCAGCATCTGA+AGG 0.570779 5:-96856900 MsG0580029427.01.T01:CDS
GAATTGCCTGAAGCAAAACA+TGG 0.573837 5:-96856987 MsG0580029427.01.T01:CDS
ACGCAATTCATTAGCTGTAG+AGG 0.575232 5:+96856956 None:intergenic
TGCAATGGTAATTGGTATGG+TGG 0.580334 5:-96854927 MsG0580029427.01.T01:CDS
CTGTGGCTATAGGCTGTCAA+AGG 0.595074 5:-96855965 MsG0580029427.01.T01:intron
TGTATTCGGTGGTGCTGCAA+TGG 0.600855 5:-96854942 MsG0580029427.01.T01:CDS
TGACCTCAAAACTAAAGGAA+AGG 0.603490 5:-96854683 MsG0580029427.01.T01:CDS
CCCTAACTGTAACATTGACA+AGG 0.605892 5:+96855928 None:intergenic
AACGACGTGTCGTTCATCAA+CGG 0.617417 5:+96857427 None:intergenic
GCCACTGCAGTTGTATTCGG+TGG 0.617882 5:-96854953 MsG0580029427.01.T01:CDS
GGTTAACATAGACGTCATAC+CGG 0.620666 5:+96856928 None:intergenic
AATTGCCTGAAGCAAAACAT+GGG 0.623883 5:-96856986 MsG0580029427.01.T01:CDS
CAGCATGTCTGAAAATACAA+AGG 0.628369 5:+96854212 None:intergenic
AGTTGAAAGCAGCATCTGAA+GGG 0.631833 5:-96856899 MsG0580029427.01.T01:CDS
ATTGCCTGAAGCAAAACATG+GGG 0.672964 5:-96856985 MsG0580029427.01.T01:CDS
GTATGACGTCTATGTTAACC+CGG 0.686842 5:-96856925 MsG0580029427.01.T01:CDS
GTTATAAGTCAGGTTGTGTG+TGG 0.689790 5:-96855706 MsG0580029427.01.T01:intron
ACCACCGAATACAACTGCAG+TGG 0.702322 5:+96854952 None:intergenic
GCCACAACAACATAAGCCAG+AGG 0.704988 5:+96854327 None:intergenic
TAGAACTTCGGGAAGCACGT+CGG 0.706030 5:+96857395 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTAAAATTAATTT+TGG + Chr5:96854584-96854603 None:intergenic 0.0%
!!! AAATTAATTTTGGTATTTTT+TGG + Chr5:96854574-96854593 None:intergenic 10.0%
!!! TATTTTTCTTCTTTATATAT+AGG + Chr5:96856163-96856182 None:intergenic 10.0%
!! AAAATAGGAATTAGAAAATA+TGG + Chr5:96856690-96856709 None:intergenic 15.0%
!! AAATAGGAATTAGAAAATAT+GGG + Chr5:96856689-96856708 None:intergenic 15.0%
!! ATATTCAAAGTATAAAGAAA+TGG + Chr5:96857114-96857133 None:intergenic 15.0%
!! CTATAAAATGAAAACAAAAT+AGG + Chr5:96856705-96856724 None:intergenic 15.0%
!! TTAGTCTACTTATATAATTA+AGG + Chr5:96856611-96856630 None:intergenic 15.0%
!!! AAAAGATCTATCTAAAATTT+TGG - Chr5:96857242-96857261 MsG0580029427.01.T01:intron 15.0%
!!! AAAGATCTATCTAAAATTTT+GGG - Chr5:96857243-96857262 MsG0580029427.01.T01:intron 15.0%
!!! ATATAATGTTTTAACAAAAC+AGG - Chr5:96855832-96855851 MsG0580029427.01.T01:intron 15.0%
!!! TATAATGTTTTAACAAAACA+GGG - Chr5:96855833-96855852 MsG0580029427.01.T01:intron 15.0%
!!! TTTTGTTTTCATTTTATAGT+TGG - Chr5:96856706-96856725 MsG0580029427.01.T01:intron 15.0%
!!! TTTTTTTCAATTCAATTTCT+AGG - Chr5:96854517-96854536 MsG0580029427.01.T01:CDS 15.0%
!! AAACTTATGTATATTAGCTA+TGG - Chr5:96855271-96855290 MsG0580029427.01.T01:intron 20.0%
!! ATGGTATATGTAGTAAATAA+CGG - Chr5:96857435-96857454 MsG0580029427.01.T01:CDS 20.0%
!! TTATTCAATAATCGCTTAAT+GGG - Chr5:96854965-96854984 MsG0580029427.01.T01:CDS 20.0%
!! TTGAATAAGTGTTTAACTAA+CGG + Chr5:96854953-96854972 None:intergenic 20.0%
!! TTGTTTGTATATCTAAACTA+TGG - Chr5:96854660-96854679 MsG0580029427.01.T01:CDS 20.0%
!!! ATAAATGTTGAGATTTTATG+AGG - Chr5:96855533-96855552 MsG0580029427.01.T01:intron 20.0%
!!! ATTTTTTGTTTGAGTGTTTT+AGG - Chr5:96854685-96854704 MsG0580029427.01.T01:CDS 20.0%
!!! TAAATGTTGAGATTTTATGA+GGG - Chr5:96855534-96855553 MsG0580029427.01.T01:intron 20.0%
!!! TTTTAAATTTATGCTTACTG+TGG - Chr5:96855716-96855735 MsG0580029427.01.T01:intron 20.0%
!!! TTTTCAATGCAAATTGAAAA+AGG + Chr5:96857310-96857329 None:intergenic 20.0%
!!! TTTTCATTTTATAGTTGGAA+AGG - Chr5:96856711-96856730 MsG0580029427.01.T01:intron 20.0%
! AAATAAAATAATCAAGTCCG+AGG - Chr5:96856183-96856202 MsG0580029427.01.T01:intron 25.0%
! AATAAAAAACAAGGTGTAAG+GGG - Chr5:96854915-96854934 MsG0580029427.01.T01:CDS 25.0%
! AATGGTCATCATTATAATCA+TGG - Chr5:96856016-96856035 MsG0580029427.01.T01:intron 25.0%
! AATTGCAAAATCCAGTTTAT+TGG - Chr5:96856953-96856972 MsG0580029427.01.T01:CDS 25.0%
! AGGATTATCATAGTTTATAG+TGG + Chr5:96855383-96855402 None:intergenic 25.0%
! ATAAATGTGAGGTTTAAACA+GGG - Chr5:96855928-96855947 MsG0580029427.01.T01:intron 25.0%
! ATGTACTAAATATCCCAAAA+TGG + Chr5:96856523-96856542 None:intergenic 25.0%
! CAATAAAAAACAAGGTGTAA+GGG - Chr5:96854914-96854933 MsG0580029427.01.T01:CDS 25.0%
! CTTATTCAATAATCGCTTAA+TGG - Chr5:96854964-96854983 MsG0580029427.01.T01:CDS 25.0%
! GAAATGCAATTATTTCACAT+AGG + Chr5:96856276-96856295 None:intergenic 25.0%
! GCAATTCTCAATAAAAAACA+AGG - Chr5:96854906-96854925 MsG0580029427.01.T01:CDS 25.0%
! GTGAAATAATTGCATTTCTA+AGG - Chr5:96856278-96856297 MsG0580029427.01.T01:intron 25.0%
! TAAGTACTTGTGTAAGTATT+TGG + Chr5:96855161-96855180 None:intergenic 25.0%
! TCAATAAAAAACAAGGTGTA+AGG - Chr5:96854913-96854932 MsG0580029427.01.T01:CDS 25.0%
! TCAATTTGCATTGAAAATAG+TGG - Chr5:96857313-96857332 MsG0580029427.01.T01:intron 25.0%
! TCTTTATATATAGGACAAGA+GGG + Chr5:96856154-96856173 None:intergenic 25.0%
! TTCTTTATATATAGGACAAG+AGG + Chr5:96856155-96856174 None:intergenic 25.0%
!! AAAACTTACCCATATTTTCA+TGG + Chr5:96857086-96857105 None:intergenic 25.0%
!! AAACTTACCCATATTTTCAT+GGG + Chr5:96857085-96857104 None:intergenic 25.0%
!! AAATGTTGAGATTTTATGAG+GGG - Chr5:96855535-96855554 MsG0580029427.01.T01:intron 25.0%
!! ACTGGATTTTGCAATTTATA+TGG + Chr5:96856949-96856968 None:intergenic 25.0%
!! ATGCCAAAACTTTGATTTAT+AGG - Chr5:96857158-96857177 MsG0580029427.01.T01:intron 25.0%
!! ATTGATAATTGGGTATTAAG+AGG - Chr5:96854628-96854647 MsG0580029427.01.T01:CDS 25.0%
!! ATTTTGTTCCCATGAAAATA+TGG - Chr5:96857074-96857093 MsG0580029427.01.T01:intron 25.0%
!! CTAGTTTGAAATTATTGCTA+AGG - Chr5:96855800-96855819 MsG0580029427.01.T01:intron 25.0%
!! CTTTTGAGCTAATTTTGTAA+CGG + Chr5:96854226-96854245 None:intergenic 25.0%
!! TCTCGTATATAAGAACATTT+TGG + Chr5:96856349-96856368 None:intergenic 25.0%
!! TGTTCCATTAATTTGAATGA+GGG - Chr5:96855583-96855602 MsG0580029427.01.T01:intron 25.0%
!! TTGATAATTGGGTATTAAGA+GGG - Chr5:96854629-96854648 MsG0580029427.01.T01:CDS 25.0%
!! TTTTGTTCCCATGAAAATAT+GGG - Chr5:96857075-96857094 MsG0580029427.01.T01:intron 25.0%
!!! ATTTTGGGATATTTAGTACA+TGG - Chr5:96856522-96856541 MsG0580029427.01.T01:intron 25.0%
!!! TTTTTGATATTCTCAAGTTG+TGG + Chr5:96857052-96857071 None:intergenic 25.0%
AACTTCACATTACATCTCTA+TGG - Chr5:96855302-96855321 MsG0580029427.01.T01:intron 30.0%
ACATTAAAGTGTAACGGTAT+AGG + Chr5:96855403-96855422 None:intergenic 30.0%
ACTTCCCTCATTCAAATTAA+TGG + Chr5:96855590-96855609 None:intergenic 30.0%
AGATGTAATGTGAAGTTTGA+TGG + Chr5:96855299-96855318 None:intergenic 30.0%
AGATTAAGAAGCCAATAAAC+TGG + Chr5:96856967-96856986 None:intergenic 30.0%
AGCAACACATTAAAGTGTAA+CGG + Chr5:96855409-96855428 None:intergenic 30.0%
AGTAAGAAGAAACAATACTG+TGG + Chr5:96856878-96856897 None:intergenic 30.0%
ATAAAAAACAAGGTGTAAGG+GGG - Chr5:96854916-96854935 MsG0580029427.01.T01:CDS 30.0%
ATAGCGTCAAGAAAAGAATA+AGG - Chr5:96856637-96856656 MsG0580029427.01.T01:intron 30.0%
CATTTGAACAGAAATTTGTG+AGG + Chr5:96856553-96856572 None:intergenic 30.0%
CTCACAAATTTCTGTTCAAA+TGG - Chr5:96856551-96856570 MsG0580029427.01.T01:intron 30.0%
GATAAATGTGAGGTTTAAAC+AGG - Chr5:96855927-96855946 MsG0580029427.01.T01:intron 30.0%
GCTGAAGAATGAAAATAGTA+TGG - Chr5:96857416-96857435 MsG0580029427.01.T01:CDS 30.0%
TAAATGTGAGGTTTAAACAG+GGG - Chr5:96855929-96855948 MsG0580029427.01.T01:intron 30.0%
TTACTTCTCTGTTATAAGTC+AGG - Chr5:96855982-96856001 MsG0580029427.01.T01:intron 30.0%
TTATACACACTATGCTCTTT+AGG - Chr5:96856471-96856490 MsG0580029427.01.T01:intron 30.0%
TTATGTTGTGCTTCAAATTC+AGG - Chr5:96856575-96856594 MsG0580029427.01.T01:intron 30.0%
! AAGCTGCAACTATTGATAAT+TGG - Chr5:96854617-96854636 MsG0580029427.01.T01:intron 30.0%
! AATGTTGAGATTTTATGAGG+GGG - Chr5:96855536-96855555 MsG0580029427.01.T01:intron 30.0%
! AGCTGCAACTATTGATAATT+GGG - Chr5:96854618-96854637 MsG0580029427.01.T01:intron 30.0%
! CAGCCTATAAATCAAAGTTT+TGG + Chr5:96857164-96857183 None:intergenic 30.0%
! CTGTTCCATTAATTTGAATG+AGG - Chr5:96855582-96855601 MsG0580029427.01.T01:intron 30.0%
! TCATTTTCTCATTGTAGGTA+AGG - Chr5:96854877-96854896 MsG0580029427.01.T01:intron 30.0%
!! AAATTGCAGTTGGTGTTATT+TGG - Chr5:96855952-96855971 MsG0580029427.01.T01:CDS 30.0%
!! AATTGCAGTTGGTGTTATTT+GGG - Chr5:96855953-96855972 MsG0580029427.01.T01:CDS 30.0%
!! CACAATTTGTTGCAATAAGA+TGG - Chr5:96857523-96857542 MsG0580029427.01.T01:CDS 30.0%
!! TAATTTTGTAACGGCTTGTT+TGG + Chr5:96854217-96854236 None:intergenic 30.0%
!! TTTGTTTGAGTGTTTTAGGT+GGG - Chr5:96854689-96854708 MsG0580029427.01.T01:CDS 30.0%
!! TTTTGTTTGAGTGTTTTAGG+TGG - Chr5:96854688-96854707 MsG0580029427.01.T01:CDS 30.0%
!!! CTTTTTTCCGTTTTAACAGA+TGG - Chr5:96856991-96857010 MsG0580029427.01.T01:CDS 30.0%
!!! TTATTGATTTTGCGTGTGTT+TGG - Chr5:96854540-96854559 MsG0580029427.01.T01:intron 30.0%
AAGAAAGTGGCATTTGGAAA+GGG - Chr5:96857194-96857213 MsG0580029427.01.T01:intron 35.0%
AATTGCCTGAAGCAAAACAT+GGG - Chr5:96854712-96854731 MsG0580029427.01.T01:intron 35.0%
ACTATGCTCTTTAGGTATCA+AGG - Chr5:96856479-96856498 MsG0580029427.01.T01:intron 35.0%
AGATACAGAAGATCGAAGTT+TGG + Chr5:96854417-96854436 None:intergenic 35.0%
AGGGAACTAAGATAAATGTG+AGG - Chr5:96855917-96855936 MsG0580029427.01.T01:intron 35.0%
AGGTCATCCATCTGTTAAAA+CGG + Chr5:96857001-96857020 None:intergenic 35.0%
ATCATTCTCTGCATCGAAAT+GGG - Chr5:96854376-96854395 MsG0580029427.01.T01:three_prime_UTR 35.0%
ATGAAACTGGTAAAACACCA+TGG - Chr5:96856068-96856087 MsG0580029427.01.T01:intron 35.0%
ATGAATAGTGCAACTTCATC+TGG + Chr5:96856424-96856443 None:intergenic 35.0%
ATGGATGACCTCAAAACTAA+AGG - Chr5:96857010-96857029 MsG0580029427.01.T01:intron 35.0%
CAAACTATTCAGAGACGAAA+CGG - Chr5:96854343-96854362 MsG0580029427.01.T01:three_prime_UTR 35.0%
CAGCATGTCTGAAAATACAA+AGG + Chr5:96857489-96857508 None:intergenic 35.0%
CCAAGACAACTTTCGAATAA+TGG - Chr5:96855887-96855906 MsG0580029427.01.T01:intron 35.0%
CCATTATTCGAAAGTTGTCT+TGG + Chr5:96855890-96855909 None:intergenic 35.0%
CCTTGTCAATGTTACAGTTA+GGG - Chr5:96855770-96855789 MsG0580029427.01.T01:intron 35.0%
CTGAGAATATGTCAAGAAAG+TGG - Chr5:96857181-96857200 MsG0580029427.01.T01:intron 35.0%
CTTGTCAATGTTACAGTTAG+GGG - Chr5:96855771-96855790 MsG0580029427.01.T01:intron 35.0%
GATACAGAAGATCGAAGTTT+GGG + Chr5:96854416-96854435 None:intergenic 35.0%
GGAAAACTAATTGTGTAGAC+AGG - Chr5:96855356-96855375 MsG0580029427.01.T01:intron 35.0%
GTCAAGTAAATACTTTGTCG+AGG + Chr5:96855250-96855269 None:intergenic 35.0%
TAAAAAACAAGGTGTAAGGG+GGG - Chr5:96854917-96854936 MsG0580029427.01.T01:CDS 35.0%
TAAAATAATCAAGTCCGAGG+AGG - Chr5:96856186-96856205 MsG0580029427.01.T01:intron 35.0%
TAAGTATTTGGAAGAGCCTA+TGG + Chr5:96855149-96855168 None:intergenic 35.0%
TATGAGGGGGATAACTTATT+GGG - Chr5:96855549-96855568 MsG0580029427.01.T01:intron 35.0%
TATGTCAAGAAAGTGGCATT+TGG - Chr5:96857188-96857207 MsG0580029427.01.T01:intron 35.0%
TCCTTGTCAATGTTACAGTT+AGG - Chr5:96855769-96855788 MsG0580029427.01.T01:intron 35.0%
TGAAACTGGTAAAACACCAT+GGG - Chr5:96856069-96856088 MsG0580029427.01.T01:intron 35.0%
TGACCTCAAAACTAAAGGAA+AGG - Chr5:96857015-96857034 MsG0580029427.01.T01:intron 35.0%
TGTGTTTCAACTAACCAACT+TGG - Chr5:96855486-96855505 MsG0580029427.01.T01:intron 35.0%
TTAAGACTTAATGACCTCCT+CGG + Chr5:96856203-96856222 None:intergenic 35.0%
TTATGAGGGGGATAACTTAT+TGG - Chr5:96855548-96855567 MsG0580029427.01.T01:intron 35.0%
TTGCATGCTATTACTACCTT+CGG + Chr5:96856372-96856391 None:intergenic 35.0%
TTTATGCTTACTGTGGCTAT+AGG - Chr5:96855723-96855742 MsG0580029427.01.T01:intron 35.0%
! AACTGTTTCGTCGATTGAAT+AGG - Chr5:96855011-96855030 MsG0580029427.01.T01:intron 35.0%
! ACAACTTTCGAATAATGGAG+AGG - Chr5:96855892-96855911 MsG0580029427.01.T01:intron 35.0%
! ACTGTTTCGTCGATTGAATA+GGG - Chr5:96855012-96855031 MsG0580029427.01.T01:intron 35.0%
! GTTTTCCATCAACTTTTAGC+TGG + Chr5:96855343-96855362 None:intergenic 35.0%
! TACATTATGCAGCTCTAACT+TGG + Chr5:96856800-96856819 None:intergenic 35.0%
! TGCTCTCATTTTCTCATTGT+AGG - Chr5:96854872-96854891 MsG0580029427.01.T01:intron 35.0%
! TGGCACATATGCTTTTCATT+CGG - Chr5:96856036-96856055 MsG0580029427.01.T01:intron 35.0%
! TTCGAATAATGGAGAGGAAA+GGG - Chr5:96855898-96855917 MsG0580029427.01.T01:intron 35.0%
! TTTCGAATAATGGAGAGGAA+AGG - Chr5:96855897-96855916 MsG0580029427.01.T01:intron 35.0%
!!! CATCCTTTCCTTTAGTTTTG+AGG + Chr5:96857021-96857040 None:intergenic 35.0%
AAGGATCCAGCAAATGCTTT+GGG - Chr5:96856656-96856675 MsG0580029427.01.T01:intron 40.0%
AAGTTGAAAGCAGCATCTGA+AGG - Chr5:96854798-96854817 MsG0580029427.01.T01:intron 40.0%
AATCAGATGCTCCCATAGTA+TGG - Chr5:96855218-96855237 MsG0580029427.01.T01:intron 40.0%
ACAAGTTTCTCTCCTGTTAC+CGG + Chr5:96855671-96855690 None:intergenic 40.0%
ACGCAATTCATTAGCTGTAG+AGG + Chr5:96854745-96854764 None:intergenic 40.0%
AGCTAAAGTTAAACCCTTGC+AGG - Chr5:96855642-96855661 MsG0580029427.01.T01:intron 40.0%
AGTTGAAAGCAGCATCTGAA+GGG - Chr5:96854799-96854818 MsG0580029427.01.T01:intron 40.0%
ATATGAATGTAGTGTGCCTC+TGG - Chr5:96857355-96857374 MsG0580029427.01.T01:intron 40.0%
ATTGCCACTGCAGTTGTATT+CGG - Chr5:96856742-96856761 MsG0580029427.01.T01:intron 40.0%
ATTGCCTGAAGCAAAACATG+GGG - Chr5:96854713-96854732 MsG0580029427.01.T01:intron 40.0%
CAAGAAAGTGGCATTTGGAA+AGG - Chr5:96857193-96857212 MsG0580029427.01.T01:intron 40.0%
CATCATTCTCTGCATCGAAA+TGG - Chr5:96854375-96854394 MsG0580029427.01.T01:three_prime_UTR 40.0%
CCCTAACTGTAACATTGACA+AGG + Chr5:96855773-96855792 None:intergenic 40.0%
CGAGGTAACATCCATACTAT+GGG + Chr5:96855232-96855251 None:intergenic 40.0%
CGATTGAATAGGGAATAAGG+AGG - Chr5:96855022-96855041 MsG0580029427.01.T01:intron 40.0%
CGTTTCGTCTCTGAATAGTT+TGG + Chr5:96854345-96854364 None:intergenic 40.0%
CTGTACCAGCTAAAAGTTGA+TGG - Chr5:96855335-96855354 MsG0580029427.01.T01:intron 40.0%
GAATTGCCTGAAGCAAAACA+TGG - Chr5:96854711-96854730 MsG0580029427.01.T01:intron 40.0%
GGTTAACATAGACGTCATAC+CGG + Chr5:96854773-96854792 None:intergenic 40.0%
GTATGACGTCTATGTTAACC+CGG - Chr5:96854773-96854792 MsG0580029427.01.T01:intron 40.0%
GTTCTTATATACGAGACCGA+AGG - Chr5:96856353-96856372 MsG0580029427.01.T01:intron 40.0%
GTTTCAACTAACCAACTTGG+CGG - Chr5:96855489-96855508 MsG0580029427.01.T01:intron 40.0%
TAAACAGGGGAAATTGCAGT+TGG - Chr5:96855942-96855961 MsG0580029427.01.T01:CDS 40.0%
TAAGGATCCAGCAAATGCTT+TGG - Chr5:96856655-96856674 MsG0580029427.01.T01:intron 40.0%
TACAGCTAATGAATTGCGTC+AGG - Chr5:96854746-96854765 MsG0580029427.01.T01:intron 40.0%
TCGAGGTAACATCCATACTA+TGG + Chr5:96855233-96855252 None:intergenic 40.0%
TCGTCTCTGAATAGTTTGGA+AGG + Chr5:96854341-96854360 None:intergenic 40.0%
TGCAATGGTAATTGGTATGG+TGG - Chr5:96856771-96856790 MsG0580029427.01.T01:intron 40.0%
TGCTGCAATGGTAATTGGTA+TGG - Chr5:96856768-96856787 MsG0580029427.01.T01:intron 40.0%
! ATAATCGCTTAATGGGCATG+TGG - Chr5:96854972-96854991 MsG0580029427.01.T01:CDS 40.0%
! ATTATGCAGCTCTAACTTGG+AGG + Chr5:96856797-96856816 None:intergenic 40.0%
! CGTCGATTGAATAGGGAATA+AGG - Chr5:96855019-96855038 MsG0580029427.01.T01:intron 40.0%
! GGCACATATGCTTTTCATTC+GGG - Chr5:96856037-96856056 MsG0580029427.01.T01:intron 40.0%
! GTTATAAGTCAGGTTGTGTG+TGG - Chr5:96855992-96856011 MsG0580029427.01.T01:intron 40.0%
! TAACGGCTTGTTTGGATTCT+TGG + Chr5:96854209-96854228 None:intergenic 40.0%
!! AAGAGAGTGTCGTAGAACTT+CGG + Chr5:96854318-96854337 None:intergenic 40.0%
!! TACTATGGGAGCATCTGATT+AGG + Chr5:96855218-96855237 None:intergenic 40.0%
!! TGAGTGATGTTCTGCCATTT+TGG - Chr5:96856506-96856525 MsG0580029427.01.T01:intron 40.0%
!!! CGTCAAGCTTTTAGAACTCA+TGG - Chr5:96856099-96856118 MsG0580029427.01.T01:intron 40.0%
!!! GAGTGATGTTCTGCCATTTT+GGG - Chr5:96856507-96856526 MsG0580029427.01.T01:intron 40.0%
AGGATCCAGCAAATGCTTTG+GGG - Chr5:96856657-96856676 MsG0580029427.01.T01:intron 45.0%
CAAGTTTCTCTCCTGTTACC+GGG + Chr5:96855670-96855689 None:intergenic 45.0%
CTAATGAATTGCGTCAGGAC+CGG - Chr5:96854751-96854770 MsG0580029427.01.T01:intron 45.0%
GGTAACAGGAGAGAAACTTG+TGG - Chr5:96855670-96855689 MsG0580029427.01.T01:CDS 45.0%
TCTCTTCCACAAGTCACCTT+TGG - Chr5:96854470-96854489 MsG0580029427.01.T01:CDS 45.0%
! AACGACGTGTCGTTCATCAA+CGG + Chr5:96854274-96854293 None:intergenic 45.0%
! AGTCAGGTTGTGTGTGGTAA+TGG - Chr5:96855998-96856017 MsG0580029427.01.T01:intron 45.0%
! ATCATCCCCAAAGCATTTGC+TGG + Chr5:96856665-96856684 None:intergenic 45.0%
! CGAAGAGGATTGATAGCATC+AGG + Chr5:96854440-96854459 None:intergenic 45.0%
! GAAACCCCATGTTTTGCTTC+AGG + Chr5:96854720-96854739 None:intergenic 45.0%
!! AGAGAGTGTCGTAGAACTTC+GGG + Chr5:96854317-96854336 None:intergenic 45.0%
!! ATGCTGCTTTCAACTTCTCC+GGG + Chr5:96854794-96854813 None:intergenic 45.0%
!! CGGAAACTGAGTTGATGTAG+TGG - Chr5:96855509-96855528 MsG0580029427.01.T01:intron 45.0%
!! GATGCTGCTTTCAACTTCTC+CGG + Chr5:96854795-96854814 None:intergenic 45.0%
!!! ACGGCGTTGGTTTTTGAGAT+TGG + Chr5:96854255-96854274 None:intergenic 45.0%
AACGAACCTTCTAGCGCCAA+AGG + Chr5:96854489-96854508 None:intergenic 50.0%
AAGTCACCTTTGGCGCTAGA+AGG - Chr5:96854480-96854499 MsG0580029427.01.T01:CDS 50.0%
ACCACCGAATACAACTGCAG+TGG + Chr5:96856749-96856768 None:intergenic 50.0%
ATACCATACTGTCGCGTCTG+GGG + Chr5:96855126-96855145 None:intergenic 50.0%
CAACTCAGTTTCCGCCAAGT+TGG + Chr5:96855503-96855522 None:intergenic 50.0%
CGCGACAGTATGGTATCCAT+AGG - Chr5:96855130-96855149 MsG0580029427.01.T01:intron 50.0%
CTGTGGCTATAGGCTGTCAA+AGG - Chr5:96855733-96855752 MsG0580029427.01.T01:intron 50.0%
GATACCATACTGTCGCGTCT+GGG + Chr5:96855127-96855146 None:intergenic 50.0%
GCCACAACAACATAAGCCAG+AGG + Chr5:96857374-96857393 None:intergenic 50.0%
GTTAAACCCTTGCAGGTTCC+CGG - Chr5:96855649-96855668 MsG0580029427.01.T01:CDS 50.0%
! AGAGAAACGAAAGCGCGAAG+AGG + Chr5:96854455-96854474 None:intergenic 50.0%
! GCCTCTGGCTTATGTTGTTG+TGG - Chr5:96857370-96857389 MsG0580029427.01.T01:intron 50.0%
! TAGAACTTCGGGAAGCACGT+CGG + Chr5:96854306-96854325 None:intergenic 50.0%
! TTTCTAGCGGGAAGGTTAGC+AGG - Chr5:96854162-96854181 MsG0580029427.01.T01:three_prime_UTR 50.0%
!! GGTGGTGCTGCAATGGTAAT+TGG - Chr5:96856763-96856782 MsG0580029427.01.T01:intron 50.0%
!! TGTATTCGGTGGTGCTGCAA+TGG - Chr5:96856756-96856775 MsG0580029427.01.T01:intron 50.0%
AGCTTGACGCACTCTTCCCA+TGG + Chr5:96856088-96856107 None:intergenic 55.0%
ATACCCCAGACGCGACAGTA+TGG - Chr5:96855120-96855139 MsG0580029427.01.T01:intron 55.0%
CTAGCGCCAAAGGTGACTTG+TGG + Chr5:96854479-96854498 None:intergenic 55.0%
CTGTTACCGGGAACCTGCAA+GGG + Chr5:96855658-96855677 None:intergenic 55.0%
GCCACTGCAGTTGTATTCGG+TGG - Chr5:96856745-96856764 MsG0580029427.01.T01:intron 55.0%
GGATACCATACTGTCGCGTC+TGG + Chr5:96855128-96855147 None:intergenic 55.0%
TCGGGCATGCTGCATGAAAC+TGG - Chr5:96856055-96856074 MsG0580029427.01.T01:intron 55.0%
! GTGTCGTTCATCAACGGCGT+TGG + Chr5:96854268-96854287 None:intergenic 55.0%
CCTGTTACCGGGAACCTGCA+AGG + Chr5:96855659-96855678 None:intergenic 60.0%
CCTTGCAGGTTCCCGGTAAC+AGG - Chr5:96855656-96855675 MsG0580029427.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 96854156 96857564 96854156 ID=MsG0580029427.01;Name=MsG0580029427.01
Chr5 mRNA 96854156 96857564 96854156 ID=MsG0580029427.01.T01;Parent=MsG0580029427.01;Name=MsG0580029427.01.T01;_AED=0.38;_eAED=0.45;_QI=0|0.33|0.57|1|1|1|7|289|225
Chr5 exon 96854156 96854540 96854156 ID=MsG0580029427.01.T01:exon:24712;Parent=MsG0580029427.01.T01
Chr5 exon 96854624 96854710 96854624 ID=MsG0580029427.01.T01:exon:24711;Parent=MsG0580029427.01.T01
Chr5 exon 96854904 96854995 96854904 ID=MsG0580029427.01.T01:exon:24710;Parent=MsG0580029427.01.T01
Chr5 exon 96855644 96855716 96855644 ID=MsG0580029427.01.T01:exon:24709;Parent=MsG0580029427.01.T01
Chr5 exon 96855935 96855975 96855935 ID=MsG0580029427.01.T01:exon:24708;Parent=MsG0580029427.01.T01
Chr5 exon 96856889 96857013 96856889 ID=MsG0580029427.01.T01:exon:24707;Parent=MsG0580029427.01.T01
Chr5 exon 96857401 96857564 96857401 ID=MsG0580029427.01.T01:exon:24706;Parent=MsG0580029427.01.T01
Chr5 CDS 96857401 96857564 96857401 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 CDS 96856889 96857013 96856889 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 CDS 96855935 96855975 96855935 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 CDS 96855644 96855716 96855644 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 CDS 96854904 96854995 96854904 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 CDS 96854624 96854710 96854624 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 CDS 96854445 96854540 96854445 ID=MsG0580029427.01.T01:cds;Parent=MsG0580029427.01.T01
Chr5 three_prime_UTR 96854156 96854444 96854156 ID=MsG0580029427.01.T01:three_prime_utr;Parent=MsG0580029427.01.T01
Gene Sequence

>MsG0580029427.01.T01

ATGAGTTTTCTAGCGGGAAGGTTAGCAGGAAAAGAAGCAGCTTATTTCTTCCAAGAATCCAAACAAGCCGTTACAAAATTAGCTCAAAAGAATAATCCAATCTCAAAAACCAACGCCGTTGATGAACGACACGTCGTTCAGATAACGCCGACGTGCTTCCCGAAGTGGGAATTGCCTGAAGCAAAACATGGGGTTTCAGCCTCTACAGCTAATGAATTGCGTCAGGACCGGTATGACGTCTATGTTAACCCGGAGAAGTTGAAAGCAGCATCTGAAGGGCTTGCAAATCGCTGTCAAAGGAATATTTTAGCGTTCCTTGTCAATGTTACAGTTGTGTGTGGTAATGGTCATCATTATAATCATGGCACATATGCTTTTCATTCGGGCATGCTGCATGAAACTGTTGGAAAGGCTTTTGCTATTGCCACTGCAGTTGTATTCGGTGGTGCTGCAATGGTAATTGGTATGGTGGCCTCCAAGTTAGAGCTGCATAATATGGATGACCTCAAAACTAAAGGAAAGGATGTTGTTGAGCCACAACTTGAGAATATCAAAAACTATTTTGTTCCCATGAAAATATGGGCTGAGAATATGTCAAGAAAGTGGCATTTGGAAAGGGAAGATGTCAAACAGAAAGCTATTGTAAAAGATCTATCTAAAATTTTGGGTTCTAAATAA
Protein sequence

>MsG0580029427.01.T01

MSFLAGRLAGKEAAYFFQESKQAVTKLAQKNNPISKTNAVDERHVVQITPTCFPKWELPEAKHGVSASTANELRQDRYDVYVNPEKLKAASEGLANRCQRNILAFLVNVTVVCGNGHHYNHGTYAFHSGMLHETVGKAFAIATAVVFGGAAMVIGMVASKLELHNMDDLKTKGKDVVEPQLENIKNYFVPMKIWAENMSRKWHLEREDVKQKAIVKDLSKILGSK*