AlfalfaGEDB | MsG0580030191.01

M. sativa cultivar ZhongmuNo.1 / MsG0580030191.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030191.01.T01	XP_024632220.1	83.333	96	16	0	8	103	199	294	9.91E-42	149
MsG0580030191.01.T02	XP_024632220.1	84.091	132	21	0	1	132	163	294	7.89E-67	215

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030191.01.T01	A0A2Z6NW35	63.158	95	35	0	9	103	300	394	1.82e-33	129
MsG0580030191.01.T02	A0A2K3L6E9	60.150	133	52	1	1	132	51	183	2.61e-54	178

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0580030191.01	MsG0380013578.01	PPI
MsG0580030191.01	MsG0280010449.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0380013578.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0780040274.01	PPI
MsG0580030191.01	MsG0280009322.01	PPI
MsG0580030191.01	MsG0280009322.01	PPI
MsG0580030191.01	MsG0780040274.01	PPI
MsG0580030191.01	MsG0280010449.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030191.01.T01	MTR_4g036855	45.833	96	24	1	8	103	23	90	1.15e-20	80.1
MsG0580030191.01.T01	MTR_7g063280	55.738	61	27	0	8	68	114	174	1.19e-18	77.0
MsG0580030191.01.T01	MTR_2g027020	37.736	106	62	1	8	113	70	171	8.77e-16	70.1
MsG0580030191.01.T01	MTR_3g010950	38.095	105	60	3	1	103	32	133	6.38e-15	66.6
MsG0580030191.01.T01	MTR_3g465840	58.491	53	22	0	8	60	17	69	1.65e-14	65.9
MsG0580030191.01.T01	MTR_4g098670	43.038	79	45	0	21	99	16	94	1.51e-13	62.0
MsG0580030191.01.T01	MTR_5g083510	41.333	75	44	0	29	103	17	91	2.22e-13	61.6
MsG0580030191.01.T01	MTR_5g031620	39.759	83	50	0	21	103	139	221	8.19e-13	62.8
MsG0580030191.01.T01	MTR_3g071490	56.863	51	22	0	53	103	9	59	3.64e-12	57.4
MsG0580030191.01.T01	MTR_1g057740	38.750	80	49	0	23	102	60	139	3.10e-11	57.0
MsG0580030191.01.T01	MTR_1g101650	40.000	80	42	2	1	80	102	175	3.57e-11	57.8
MsG0580030191.01.T01	MTR_6g034915	34.737	95	45	1	8	102	97	174	5.24e-11	57.4
MsG0580030191.01.T01	MTR_5g022720	39.437	71	43	0	14	84	2	72	5.72e-11	58.2
MsG0580030191.01.T01	MTR_1g052310	39.506	81	49	0	22	102	42	122	8.50e-11	55.8
MsG0580030191.01.T01	MTR_2g088140	34.940	83	54	0	10	92	32	114	9.81e-11	55.5
MsG0580030191.01.T02	MTR_7g063280	57.732	97	41	0	1	97	78	174	2.39e-40	134
MsG0580030191.01.T02	MTR_2g027020	42.553	141	77	1	2	142	35	171	5.15e-32	113
MsG0580030191.01.T02	MTR_0015s0120	79.412	68	14	0	1	68	299	366	1.04e-31	116
MsG0580030191.01.T02	MTR_5g049680	67.606	71	22	1	1	70	37	107	1.61e-29	104
MsG0580030191.01.T02	MTR_1g045560	44.737	114	44	2	1	114	1	95	2.15e-29	103
MsG0580030191.01.T02	MTR_6g016265	55.556	81	36	0	1	81	45	125	2.22e-29	104
MsG0580030191.01.T02	MTR_4g036855	47.863	117	33	1	16	132	2	90	4.47e-28	100
MsG0580030191.01.T02	MTR_5g091440	43.182	132	42	1	1	132	1	99	5.85e-28	100
MsG0580030191.01.T02	MTR_5g075610	45.455	99	52	1	1	99	1	97	8.62e-26	94.4
MsG0580030191.01.T02	MTR_6g034915	38.168	131	64	1	1	131	61	174	8.05e-25	94.4
MsG0580030191.01.T02	MTR_7g053400	43.519	108	27	1	1	108	181	254	4.40e-24	94.4
MsG0580030191.01.T02	MTR_8g098925	45.652	92	50	0	1	92	71	162	2.12e-23	90.1
MsG0580030191.01.T02	MTR_1g074090	63.934	61	22	0	1	61	87	147	2.37e-23	89.7
MsG0580030191.01.T02	MTR_8g088220	48.684	76	39	0	5	80	4	79	4.03e-22	84.3
MsG0580030191.01.T02	MTR_1g088650	45.918	98	53	0	1	98	92	189	5.43e-22	87.4
MsG0580030191.01.T02	MTR_7g072030	70.370	54	16	0	15	68	69	122	1.14e-21	84.7
MsG0580030191.01.T02	MTR_3g465840	57.971	69	29	0	21	89	1	69	2.41e-21	84.7
MsG0580030191.01.T02	MTR_6g453270	51.351	74	36	0	1	74	306	379	2.82e-21	88.6
MsG0580030191.01.T02	MTR_1g081930	48.148	81	42	0	1	81	44	124	9.81e-21	82.4
MsG0580030191.01.T02	MTR_4g008270	47.945	73	38	0	1	73	16	88	1.25e-20	81.3
MsG0580030191.01.T02	MTR_5g037920	39.024	123	73	2	1	122	104	225	1.29e-20	84.7
MsG0580030191.01.T02	MTR_6g016135	65.517	58	20	0	1	58	385	442	2.43e-20	86.3
MsG0580030191.01.T02	MTR_7g013520	38.636	132	56	1	1	132	76	182	2.55e-20	82.8
MsG0580030191.01.T02	MTR_1g069475	52.778	72	34	0	2	73	171	242	3.10e-20	84.0
MsG0580030191.01.T02	MTR_2g059470	46.154	78	42	0	1	78	6	83	4.90e-20	79.3
MsG0580030191.01.T02	MTR_3g026270	56.364	55	24	0	1	55	1	55	7.22e-20	78.2
MsG0580030191.01.T02	MTR_1g034430	44.444	81	45	0	1	81	101	181	1.51e-19	80.9
MsG0580030191.01.T02	MTR_0093s0070	46.753	77	41	0	1	77	129	205	1.52e-19	81.3
MsG0580030191.01.T02	MTR_3g116210	42.353	85	49	0	1	85	103	187	1.71e-19	80.9
MsG0580030191.01.T02	MTR_8g031080	38.462	130	54	1	1	130	295	398	2.79e-19	83.2
MsG0580030191.01.T02	MTR_8g031800	48.052	77	39	1	1	77	37	112	2.81e-19	78.2
MsG0580030191.01.T02	MTR_6g089560	34.848	132	64	1	1	132	122	231	2.49e-18	78.6
MsG0580030191.01.T02	MTR_5g083200	41.379	87	51	0	1	87	104	190	8.14e-18	76.6
MsG0580030191.01.T02	MTR_0002s1220	41.379	87	51	0	1	87	104	190	8.14e-18	76.6
MsG0580030191.01.T02	MTR_3g111390	41.379	87	51	0	1	87	104	190	1.14e-17	76.3
MsG0580030191.01.T02	MTR_1g070820	41.379	87	51	0	1	87	104	190	1.14e-17	76.3
MsG0580030191.01.T02	MTR_6g013970	36.449	107	65	2	3	108	107	211	1.75e-17	76.3
MsG0580030191.01.T02	MTR_1g045340	40.230	87	52	0	1	87	90	176	2.20e-17	75.1
MsG0580030191.01.T02	MTR_5g085210	36.697	109	53	1	1	109	125	217	8.23e-17	74.7
MsG0580030191.01.T02	MTR_5g069700	47.143	70	37	0	16	85	2	71	4.74e-16	68.9
MsG0580030191.01.T02	MTR_7g006380	36.607	112	44	1	1	112	72	156	1.04e-15	70.9
MsG0580030191.01.T02	MTR_5g034450	51.724	58	28	0	1	58	55	112	1.80e-15	68.6
MsG0580030191.01.T02	MTR_1g050488	54.237	59	27	0	2	60	105	163	1.91e-15	69.7
MsG0580030191.01.T02	MTR_3g010950	33.333	132	58	2	1	132	32	133	2.49e-15	68.6
MsG0580030191.01.T02	MTR_1g074240	45.902	61	33	0	1	61	6	66	1.16e-14	65.1
MsG0580030191.01.T02	MTR_8g059135	43.243	74	42	0	1	74	34	107	1.18e-14	66.6
MsG0580030191.01.T02	MTR_4g029360	39.785	93	56	0	3	95	135	227	1.80e-14	68.2
MsG0580030191.01.T02	MTR_1g062840	44.118	68	38	0	20	87	5	72	2.13e-14	65.1
MsG0580030191.01.T02	MTR_5g036320	52.542	59	28	0	1	59	85	143	2.90e-14	66.2
MsG0580030191.01.T02	MTR_8g038340	59.615	52	21	0	1	52	195	246	4.12e-14	67.8
MsG0580030191.01.T02	MTR_8g099580	46.575	73	39	0	1	73	212	284	4.69e-14	68.2
MsG0580030191.01.T02	MTR_1g017840	41.772	79	46	0	1	79	84	162	5.08e-14	65.9
MsG0580030191.01.T02	MTR_4g098670	43.038	79	45	0	50	128	16	94	2.22e-13	62.4
MsG0580030191.01.T02	MTR_5g083510	41.333	75	44	0	58	132	17	91	4.99e-13	61.6
MsG0580030191.01.T02	MTR_8g028785	44.304	79	44	0	24	102	287	365	5.38e-13	65.5
MsG0580030191.01.T02	MTR_7g082130	34.545	110	47	2	1	109	50	135	5.40e-13	62.4
MsG0580030191.01.T02	MTR_8g036780	45.312	64	35	0	1	64	40	103	8.00e-13	61.6
MsG0580030191.01.T02	MTR_3g090920	33.333	123	66	3	10	132	2	108	1.09e-12	61.2
MsG0580030191.01.T02	MTR_5g031620	39.759	83	50	0	50	132	139	221	1.68e-12	62.8
MsG0580030191.01.T02	MTR_1g017820	55.556	54	24	0	7	60	12	65	3.06e-12	58.9
MsG0580030191.01.T02	MTR_3g071490	56.863	51	22	0	82	132	9	59	6.15e-12	57.8
MsG0580030191.01.T02	MTR_6g045427	45.902	61	33	0	27	87	177	237	9.42e-12	61.2
MsG0580030191.01.T02	MTR_4g073820	55.556	54	22	1	1	54	1	52	3.48e-11	56.2
MsG0580030191.01.T02	MTR_1g057740	38.750	80	49	0	52	131	60	139	4.28e-11	57.8
MsG0580030191.01.T02	MTR_2g047100	34.043	94	47	1	1	94	24	102	7.95e-11	55.8
MsG0580030191.01.T02	MTR_5g022720	39.437	71	43	0	43	113	2	72	8.42e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAGCTAGTTTCGATATAT+TGG	0.232744	5:+108035469	MsG0580030191.01.T02:CDS
TGAAAAGAGCTTTACATCTA+TGG	0.241131	5:-108035361	None:intergenic
ATATCGAAACTAGCTGATAA+TGG	0.313143	5:-108035464	None:intergenic
ACTTTAGTTTAGAGTTTATT+CGG	0.321228	5:+108035399	MsG0580030191.01.T02:CDS
TATCGAAACTAGCTGATAAT+GGG	0.340504	5:-108035463	None:intergenic
CAACATGATGCTACAAAGTT+TGG	0.357816	5:+108035329	MsG0580030191.01.T02:CDS
GTTTACTATCTGCTTTAGAA+TGG	0.361143	5:+108035231	MsG0580030191.01.T02:CDS
TCCTCTTTGTGATGTTTATG+TGG	0.413217	5:+108035196	MsG0580030191.01.T02:CDS
TTTACTATCTGCTTTAGAAT+GGG	0.416565	5:+108035232	MsG0580030191.01.T02:CDS
TAGCATCATGTTGTGCAGAT+AGG	0.432726	5:-108035319	None:intergenic
GGGCCGCCTTAGCTAATTCA+TGG	0.434963	5:-108035442	None:intergenic
TTGTGTTGGCTAAAACTGAA+TGG	0.440514	5:+108035168	MsG0580030191.01.T02:CDS
ATGTGTGTCAGAGATGATTC+AGG	0.440804	5:+108035140	MsG0580030191.01.T02:CDS
GCGTCAAGCTCAAAATCAAT+AGG	0.462768	5:-108035275	None:intergenic
TGATTCAGGTTCCTTTGTGT+TGG	0.473472	5:+108035154	MsG0580030191.01.T02:CDS
AAAATGTGTTCAATACAATC+TGG	0.490257	5:-108035500	None:intergenic
TGGGTTCCTGATCGTCAACT+TGG	0.490670	5:+108035251	MsG0580030191.01.T02:CDS
ATAGGTCCAAGTTGACGATC+AGG	0.496773	5:-108035257	None:intergenic
GTTTATGTGGGAGAAGCTCT+TGG	0.502581	5:+108035209	MsG0580030191.01.T02:CDS
CCTCTTTGTGATGTTTATGT+GGG	0.526673	5:+108035197	MsG0580030191.01.T02:CDS
GGCCGCCTTAGCTAATTCAT+GGG	0.601473	5:-108035441	None:intergenic
CCCACATAAACATCACAAAG+AGG	0.617880	5:-108035197	None:intergenic
TGCCCATGAATTAGCTAAGG+CGG	0.623182	5:+108035439	MsG0580030191.01.T02:CDS
GGTTGCCCATGAATTAGCTA+AGG	0.624042	5:+108035436	MsG0580030191.01.T02:CDS
TATTCGGAGACAAGCACATG+AGG	0.635570	5:+108035415	MsG0580030191.01.T02:CDS
ATCGAAACTAGCTGATAATG+GGG	0.639074	5:-108035462	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTTTAGTTTAGAGTTTATT+CGG	+	Chr5:108035399-108035418	MsG0580030191.01.T02:CDS	20.0%
!	AAAATGTGTTCAATACAATC+TGG	-	Chr5:108035503-108035522	None:intergenic	25.0%
!!!	TTTACTATCTGCTTTAGAAT+GGG	+	Chr5:108035232-108035251	MsG0580030191.01.T02:CDS	25.0%
	ATATCGAAACTAGCTGATAA+TGG	-	Chr5:108035467-108035486	None:intergenic	30.0%
	TATCGAAACTAGCTGATAAT+GGG	-	Chr5:108035466-108035485	None:intergenic	30.0%
	TGAAAAGAGCTTTACATCTA+TGG	-	Chr5:108035364-108035383	None:intergenic	30.0%
!	ATCAGCTAGTTTCGATATAT+TGG	+	Chr5:108035469-108035488	MsG0580030191.01.T02:CDS	30.0%
!!	GTTTACTATCTGCTTTAGAA+TGG	+	Chr5:108035231-108035250	MsG0580030191.01.T02:CDS	30.0%
	CAACATGATGCTACAAAGTT+TGG	+	Chr5:108035329-108035348	MsG0580030191.01.T02:CDS	35.0%
	CCTCTTTGTGATGTTTATGT+GGG	+	Chr5:108035197-108035216	MsG0580030191.01.T02:CDS	35.0%
	TCCTCTTTGTGATGTTTATG+TGG	+	Chr5:108035196-108035215	MsG0580030191.01.T02:CDS	35.0%
!	ATCGAAACTAGCTGATAATG+GGG	-	Chr5:108035465-108035484	None:intergenic	35.0%
!!	TTCAGTTTTAGCCAACACAA+AGG	-	Chr5:108035168-108035187	None:intergenic	35.0%
!!	TTGTGTTGGCTAAAACTGAA+TGG	+	Chr5:108035168-108035187	MsG0580030191.01.T02:CDS	35.0%
	ATGTGTGTCAGAGATGATTC+AGG	+	Chr5:108035140-108035159	MsG0580030191.01.T02:CDS	40.0%
	CCCACATAAACATCACAAAG+AGG	-	Chr5:108035200-108035219	None:intergenic	40.0%
	GCGTCAAGCTCAAAATCAAT+AGG	-	Chr5:108035278-108035297	None:intergenic	40.0%
	TAGCATCATGTTGTGCAGAT+AGG	-	Chr5:108035322-108035341	None:intergenic	40.0%
!	TGATTCAGGTTCCTTTGTGT+TGG	+	Chr5:108035154-108035173	MsG0580030191.01.T02:CDS	40.0%
	GGTTGCCCATGAATTAGCTA+AGG	+	Chr5:108035436-108035455	MsG0580030191.01.T02:CDS	45.0%
	GTTTATGTGGGAGAAGCTCT+TGG	+	Chr5:108035209-108035228	MsG0580030191.01.T02:CDS	45.0%
	TATTCGGAGACAAGCACATG+AGG	+	Chr5:108035415-108035434	MsG0580030191.01.T02:CDS	45.0%
	TGCCCATGAATTAGCTAAGG+CGG	+	Chr5:108035439-108035458	MsG0580030191.01.T02:CDS	45.0%
!	ATAGGTCCAAGTTGACGATC+AGG	-	Chr5:108035260-108035279	None:intergenic	45.0%
	GGCCGCCTTAGCTAATTCAT+GGG	-	Chr5:108035444-108035463	None:intergenic	50.0%
	TGGGTTCCTGATCGTCAACT+TGG	+	Chr5:108035251-108035270	MsG0580030191.01.T02:CDS	50.0%
	GGGCCGCCTTAGCTAATTCA+TGG	-	Chr5:108035445-108035464	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	108035140	108035598	108035140	ID=MsG0580030191.01;Name=MsG0580030191.01
Chr5	mRNA	108035140	108035598	108035140	ID=MsG0580030191.01.T01;Parent=MsG0580030191.01;Name=MsG0580030191.01.T01;_AED=0.50;_eAED=0.53;_QI=0\|0\|0\|1\|0\|0\|2\|0\|123
Chr5	exon	108035140	108035161	108035140	ID=MsG0580030191.01.T01:exon:15196;Parent=MsG0580030191.01.T01
Chr5	exon	108035249	108035598	108035249	ID=MsG0580030191.01.T01:exon:15197;Parent=MsG0580030191.01.T01
Chr5	CDS	108035140	108035161	108035140	ID=MsG0580030191.01.T01:cds;Parent=MsG0580030191.01.T01
Chr5	CDS	108035249	108035598	108035249	ID=MsG0580030191.01.T01:cds;Parent=MsG0580030191.01.T01
Chr5	mRNA	108035140	108035598	108035140	ID=MsG0580030191.01.T02;Parent=MsG0580030191.01;Name=MsG0580030191.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|152
Chr5	exon	108035140	108035598	108035140	ID=MsG0580030191.01.T02:exon:15198;Parent=MsG0580030191.01.T02
Chr5	CDS	108035140	108035598	108035140	ID=MsG0580030191.01.T02:cds;Parent=MsG0580030191.01.T02

Gene Sequence

>MsG0580030191.01.T01

ATGTGTGTCAGAGATGATTCAGAATGGGTTCCTGATCGTCAACTTGGACCTATTGATTTTGAGCTTGACGCGAAAAGAGTTGTTGACAACTTCCTATCTGCACAACATGATGCTACAAAGTTTGGTAGTATTTTCCATAGATGTAAAGCTCTTTTCAATTTATTTTATGAAAACTTTAGTTTAGAGTTTATTCGGAGACAAGCACATGAGGTTGCCCATGAATTAGCTAAGGCGGCCCCATTATCAGCTAGTTTCGATATATTGGTTAACATACCAGATTGTATTGAACACATTTTAAGTAATAAAATGTATAAGTCTATTTCTTTAAAAAAAAAAAAACTCAACATTTGTCAATACTTTCGAAGTAAATAA

>MsG0580030191.01.T02

ATGTGTGTCAGAGATGATTCAGGTTCCTTTGTGTTGGCTAAAACTGAATGGATTATTCCTCTTTGTGATGTTTATGTGGGAGAAGCTCTTGGTTTACTATCTGCTTTAGAATGGGTTCCTGATCGTCAACTTGGACCTATTGATTTTGAGCTTGACGCGAAAAGAGTTGTTGACAACTTCCTATCTGCACAACATGATGCTACAAAGTTTGGTAGTATTTTCCATAGATGTAAAGCTCTTTTCAATTTATTTTATGAAAACTTTAGTTTAGAGTTTATTCGGAGACAAGCACATGAGGTTGCCCATGAATTAGCTAAGGCGGCCCCATTATCAGCTAGTTTCGATATATTGGTTAACATACCAGATTGTATTGAACACATTTTAAGTAATAAAATGTATAAGTCTATTTCTTTAAAAAAAAAAAAACTCAACATTTGTCAATACTTTCGAAGTAAATAA

Protein sequence

>MsG0580030191.01.T01

MCVRDDSEWVPDRQLGPIDFELDAKRVVDNFLSAQHDATKFGSIFHRCKALFNLFYENFSLEFIRRQAHEVAHELAKAAPLSASFDILVNIPDCIEHILSNKMYKSISLKKKKLNICQYFRSK*

>MsG0580030191.01.T02

MCVRDDSGSFVLAKTEWIIPLCDVYVGEALGLLSALEWVPDRQLGPIDFELDAKRVVDNFLSAQHDATKFGSIFHRCKALFNLFYENFSLEFIRRQAHEVAHELAKAAPLSASFDILVNIPDCIEHILSNKMYKSISLKKKKLNICQYFRSK*