AlfalfaGEDB | MsG0680030322.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030322.01

KEGG: K01792
GO: GO:0003824 GO:0005975 GO:0016853 GO:0030246
PFAM: PF01263

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030322.01.T01	XP_013450738.2	97.772	359	7	1	5	363	1	358	0	723

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030322.01.T01	Q40784	58.389	298	119	4	69	361	32	329	6.01E-118	347

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030322.01.T01	A0A072U4Z1	98.300	353	5	1	11	363	1	352	0.0	714

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0580024360.01	MsG0680030322.01	PPI
MsG0680030322.01	MsG0280008234.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030322.01.T01	MTR_6g004520	98.300	353	5	1	11	363	1	352	0.0	714
MsG0680030322.01.T01	MTR_6g004520	97.923	337	6	1	27	363	28	363	0.0	682
MsG0680030322.01.T01	MTR_6g004520	98.689	305	4	0	59	363	1	305	0.0	625
MsG0680030322.01.T01	MTR_2g036810	60.870	299	112	4	68	361	19	317	8.42e-129	372
MsG0680030322.01.T01	MTR_4g005740	59.060	298	117	4	68	360	15	312	1.25e-123	358
MsG0680030322.01.T01	MTR_7g116910	55.290	293	127	3	65	353	18	310	9.08e-117	341
MsG0680030322.01.T01	MTR_4g092780	54.949	293	128	3	65	353	18	310	1.64e-114	335
MsG0680030322.01.T01	MTR_5g009670	53.448	290	132	3	61	347	15	304	1.63e-112	330
MsG0680030322.01.T01	MTR_5g009670	52.564	234	108	3	61	291	15	248	8.34e-86	260
MsG0680030322.01.T01	MTR_5g020640	31.359	287	179	7	59	342	39	310	7.10e-35	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030322.01.T01	AT5G14500	69.737	304	91	1	59	361	1	304	9.39e-159	447
MsG0680030322.01.T01	AT3G01590	68.750	304	94	1	59	361	1	304	4.90e-156	441
MsG0680030322.01.T01	AT3G01590	68.750	304	94	1	59	361	1	304	4.90e-156	441
MsG0680030322.01.T01	AT5G14500	64.438	329	91	2	59	361	1	329	2.69e-152	432
MsG0680030322.01.T01	AT5G57330	60.403	298	111	5	68	359	15	311	5.53e-124	360
MsG0680030322.01.T01	AT3G61610	53.041	296	135	3	63	354	16	311	1.86e-111	328
MsG0680030322.01.T01	AT3G61610	53.041	296	135	3	63	354	10	305	2.09e-111	327
MsG0680030322.01.T01	AT4G23730	52.740	292	133	3	61	347	15	306	4.62e-107	316
MsG0680030322.01.T01	AT4G23730	52.013	298	132	4	61	347	15	312	3.42e-105	312
MsG0680030322.01.T01	AT4G23730	54.647	269	117	3	84	347	3	271	3.07e-101	300

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Find 86 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACATAAAGCAATCAG+AGG	0.644716	6:-600863	MsG0680030322.01.T01:CDS
AAACATAAAGTGAGGATAAA+AGG	0.265244	6:+599129	None:intergenic
AAACCATAAAGAACTCGACG+AGG	0.748460	6:+599570	None:intergenic
AACATCTCTTAATTATTGTA+AGG	0.410691	6:+599332	None:intergenic
AACTAATAGTAACCTGCATC+TGG	0.440068	6:+599864	None:intergenic
AAGAAACAGGATGTGGTCAT+TGG	0.451987	6:-600661	MsG0680030322.01.T01:CDS
AAGAATTCACTTCCAGATGC+AGG	0.502151	6:-599876	MsG0680030322.01.T01:intron
AAGACAAAGATGGATTGCCA+AGG	0.592129	6:-601780	MsG0680030322.01.T01:CDS
AAGCCATGTGAAGAATGGAA+AGG	0.627246	6:-599645	MsG0680030322.01.T01:CDS
AAGCTATTGCAGTGGACAAT+TGG	0.418644	6:-599595	MsG0680030322.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACCACTGACAAATTTG+TGG	+	Chr6:600133-600152	None:intergenic	30.0%
AAACAACATAAAGCAATCAG+AGG	-	Chr6:600252-600271	MsG0680030322.01.T01:intron	30.0%
AAATATTATTTGGACCGGTA+TGG	-	Chr6:599895-599914	MsG0680030322.01.T01:CDS	30.0%
AATAGACAATCATTCATCTG+TGG	-	Chr6:600502-600521	MsG0680030322.01.T01:intron	30.0%
ACAGATGAATGATTGTCTAT+TGG	+	Chr6:600503-600522	None:intergenic	30.0%
ATATGTATACACAGCATTAC+TGG	+	Chr6:601897-601916	None:intergenic	30.0%
ATGCAGGTTACTATTAGTTA+GGG	-	Chr6:601255-601274	MsG0680030322.01.T01:intron	30.0%
ATGGAATCCATGGAATAAAA+AGG	-	Chr6:601361-601380	MsG0680030322.01.T01:intron	30.0%
CAATGAAAATCTTCAGAGAA+TGG	-	Chr6:601868-601887	MsG0680030322.01.T01:CDS	30.0%
GGAAAAAAAGTGAAAATCAG+TGG	+	Chr6:600886-600905	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	599142	601995	599142	ID=MsG0680030322.01;Name=MsG0680030322.01
Chr6	mRNA	599142	601995	599142	ID=MsG0680030322.01.T01;Parent=MsG0680030322.01;Name=MsG0680030322.01.T01;_AED=0.28;_eAED=0.29;_QI=0\|1\|0.87\|1\|1\|1\|8\|421\|363
Chr6	exon	599142	599780	599142	ID=MsG0680030322.01.T01:exon:2190;Parent=MsG0680030322.01.T01
Chr6	exon	599877	599976	599877	ID=MsG0680030322.01.T01:exon:2189;Parent=MsG0680030322.01.T01
Chr6	exon	600050	600152	600050	ID=MsG0680030322.01.T01:exon:2188;Parent=MsG0680030322.01.T01
Chr6	exon	600269	600400	600269	ID=MsG0680030322.01.T01:exon:2187;Parent=MsG0680030322.01.T01
Chr6	exon	600567	600721	600567	ID=MsG0680030322.01.T01:exon:2186;Parent=MsG0680030322.01.T01
Chr6	exon	600838	600894	600838	ID=MsG0680030322.01.T01:exon:2185;Parent=MsG0680030322.01.T01
Chr6	exon	601070	601144	601070	ID=MsG0680030322.01.T01:exon:2184;Parent=MsG0680030322.01.T01
Chr6	exon	601744	601995	601744	ID=MsG0680030322.01.T01:exon:2183;Parent=MsG0680030322.01.T01
Chr6	CDS	601744	601995	601744	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	601070	601144	601070	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	600838	600894	600838	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	600567	600721	600567	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	600269	600400	600269	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	600050	600152	600050	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	599877	599976	599877	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	CDS	599563	599780	599563	ID=MsG0680030322.01.T01:cds;Parent=MsG0680030322.01.T01
Chr6	three_prime_UTR	599142	599562	599142	ID=MsG0680030322.01.T01:three_prime_utr;Parent=MsG0680030322.01.T01

Gene Sequence

>MsG0680030322.01.T01

ATGAATATTATGTTGATGTTGTTATGGTGGATGGTGAAGATGAAATTGAGAACATTGTTTGGTTTTTTTGATTGGTTGCAGAACCTGGATTGGAGAAGAGTGAAAGTGAAGCCATTTGTGAGTAATTCAAGAGCATTAAGGTTGCAGCAACAGCAGCATCAACCAATTATACAGATGCCTTTGAATGTATTTCAAGACAAAGATGGATTGCCAAGGATTGTATTGACTGATCCAAATGGTTCATCAGCTGAGGTGCTTCTATATGGAGGGCATATTGTTTCTTGGAAGAATCAAAGGAAGGAAGAACTGCTTTTTATGAGCAGTAAGGCTAAATGGAAACAACATAAAGCAATCAGAGGAGGTATATCAGCTTGCTTCGCACGGTTTGGTGATGTTGGTTCACTTGAGACACATGGAATGACAAGAAACAGGATGTGGTCATTGGATAGAGACCCTTCACCACTACCTCCAATAGACAATCATTCATCTGTGGATTTGATATTAAAGTCAACAGGAGTTGACTTAAAGACGCCACGAAGTTTTGAGTTGAGGCTTAGGATATCTCTAAGTGCTGGCAAGCTCATTCTGATTCCTAGAGTCAGAAACACTGATAACAAACCATTTTCTTTTGCATTTTCACTATGCAACTTTTTATCTGTATCAGATATCAGTGAAGTGCGTATCGAGGGATTAGAGACACTTGATTATGTTGATAATCTGATGAACAGATCAAGATTCACAGAACAGGCTGATGCAATTACATTTGATGGAGAGACGGACAGAGTATACTTGCACAGCCCAAATAAAATTGCCATCATAGATCATGAGAAGAAAAGAACTTTTGTACTCCAGAAGAATTCACTTCCAGATGCAGTTGTATGGAATCCATGGAATAAAAAGGCTAAAGCTCTACCTGATTTGGGAGTTGATGATTACAAAATGATGTTATGTGTGAATTCAGCAGCTATTGATACTCCTATTATCTTGAAGCCATGTGAAGAATGGAAAGGTTATCAAGAAATCTCTACTGTTTCGTCAAGCTATTGCAGTGGACAATTGGATCCTCGTCGAGTTCTTTATGGTTTTCAGTGA

Protein sequence

>MsG0680030322.01.T01

MNIMLMLLWWMVKMKLRTLFGFFDWLQNLDWRRVKVKPFVSNSRALRLQQQQHQPIIQMPLNVFQDKDGLPRIVLTDPNGSSAEVLLYGGHIVSWKNQRKEELLFMSSKAKWKQHKAIRGGISACFARFGDVGSLETHGMTRNRMWSLDRDPSPLPPIDNHSSVDLILKSTGVDLKTPRSFELRLRISLSAGKLILIPRVRNTDNKPFSFAFSLCNFLSVSDISEVRIEGLETLDYVDNLMNRSRFTEQADAITFDGETDRVYLHSPNKIAIIDHEKKRTFVLQKNSLPDAVVWNPWNKKAKALPDLGVDDYKMMLCVNSAAIDTPIILKPCEEWKGYQEISTVSSSYCSGQLDPRRVLYGFQ*