Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030598.01.T01 | PRQ53836.1 | 70.085 | 117 | 35 | 0 | 1 | 117 | 15 | 131 | 1.80E-47 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030598.01.T01 | Q9FL07 | 75.281 | 89 | 21 | 1 | 29 | 117 | 38 | 125 | 3.69E-38 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030598.01.T01 | A0A2P6S586 | 70.085 | 117 | 35 | 0 | 1 | 117 | 15 | 131 | 8.59e-48 | 166 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680030597.01 | MsG0680030598.01 | 0.805968 | 1.033346e-49 | 5.541989e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030598.01.T01 | MTR_6g007737 | 88.235 | 119 | 11 | 2 | 1 | 117 | 34 | 151 | 7.15e-47 | 155 |
| MsG0680030598.01.T01 | MTR_5g034790 | 68.041 | 97 | 27 | 1 | 25 | 117 | 38 | 134 | 7.92e-32 | 115 |
| MsG0680030598.01.T01 | MTR_4g106680 | 51.304 | 115 | 45 | 4 | 5 | 117 | 19 | 124 | 1.66e-25 | 97.8 |
| MsG0680030598.01.T01 | MTR_8g063660 | 59.756 | 82 | 33 | 0 | 36 | 117 | 5 | 86 | 2.53e-24 | 95.1 |
| MsG0680030598.01.T01 | MTR_4g129420 | 58.025 | 81 | 26 | 2 | 31 | 111 | 57 | 129 | 3.04e-18 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030598.01.T01 | AT5G40250 | 75.281 | 89 | 21 | 1 | 29 | 117 | 38 | 125 | 3.77e-39 | 134 |
| MsG0680030598.01.T01 | AT1G23980 | 60.920 | 87 | 32 | 1 | 27 | 111 | 44 | 130 | 5.23e-28 | 105 |
| MsG0680030598.01.T01 | AT5G57750 | 41.860 | 86 | 46 | 2 | 32 | 117 | 32 | 113 | 4.32e-14 | 65.9 |
| MsG0680030598.01.T01 | AT2G18650 | 49.438 | 89 | 38 | 3 | 29 | 117 | 36 | 117 | 1.02e-12 | 63.5 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAATTCAAGGAGATTGT+TGG | 0.419005 | 6:-5838608 | MsG0680030598.01.T01:CDS |
| TTAGAAGAACTTGATGAAGA+TGG | 0.478882 | 6:+5838748 | None:intergenic |
| CTAAACCAGAATCATGAAGA+TGG | 0.497625 | 6:+5838659 | None:intergenic |
| CTTGATGAAGATTGTGATGA+TGG | 0.502333 | 6:+5838922 | None:intergenic |
| ACTATGATGAAAAGAACAGC+TGG | 0.524123 | 6:+5838838 | None:intergenic |
| TACTAGAAGATGAAGATGGT+GGG | 0.528489 | 6:+5838872 | None:intergenic |
| ACTAGAAGATGAAGATGGTG+GGG | 0.564302 | 6:+5838873 | None:intergenic |
| TTAGTACTAGAAGATGAAGA+TGG | 0.602759 | 6:+5838868 | None:intergenic |
| GAAGATTGTGATGATGGTGG+TGG | 0.606961 | 6:+5838928 | None:intergenic |
| GTACTAGAAGATGAAGATGG+TGG | 0.612334 | 6:+5838871 | None:intergenic |
| GATGAAGATTGTGATGATGG+TGG | 0.694129 | 6:+5838925 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATCTCCTTGAATTGAAAAA+AGG | + | Chr6:5838930-5838949 | None:intergenic | 25.0% |
| TTAGAAGAACTTGATGAAGA+TGG | + | Chr6:5838795-5838814 | None:intergenic | 30.0% | |
| TTAGTACTAGAAGATGAAGA+TGG | + | Chr6:5838675-5838694 | None:intergenic | 30.0% | |
| TTTCAATTCAAGGAGATTGT+TGG | - | Chr6:5838932-5838951 | MsG0680030598.01.T01:CDS | 30.0% | |
| !!! | CACTACCTTTTTTCAATTCA+AGG | - | Chr6:5838922-5838941 | MsG0680030598.01.T01:CDS | 30.0% |
| !!! | GCTGTTTTGTTTTTCATATC+TGG | - | Chr6:5838732-5838751 | MsG0680030598.01.T01:CDS | 30.0% |
| ACTATGATGAAAAGAACAGC+TGG | + | Chr6:5838705-5838724 | None:intergenic | 35.0% | |
| CTAAACCAGAATCATGAAGA+TGG | + | Chr6:5838884-5838903 | None:intergenic | 35.0% | |
| CTTGATGAAGATTGTGATGA+TGG | + | Chr6:5838621-5838640 | None:intergenic | 35.0% | |
| TACTAGAAGATGAAGATGGT+GGG | + | Chr6:5838671-5838690 | None:intergenic | 35.0% | |
| ! | AGCTGTTCTTTTCATCATAG+TGG | - | Chr6:5838704-5838723 | MsG0680030598.01.T01:CDS | 35.0% |
| ! | CTTTTCCATCTTCATGATTC+TGG | - | Chr6:5838876-5838895 | MsG0680030598.01.T01:CDS | 35.0% |
| ACTAGAAGATGAAGATGGTG+GGG | + | Chr6:5838670-5838689 | None:intergenic | 40.0% | |
| GTACTAGAAGATGAAGATGG+TGG | + | Chr6:5838672-5838691 | None:intergenic | 40.0% | |
| !! | GATGAAGATTGTGATGATGG+TGG | + | Chr6:5838618-5838637 | None:intergenic | 40.0% |
| !! | GAAGATTGTGATGATGGTGG+TGG | + | Chr6:5838615-5838634 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 5838603 | 5838959 | 5838603 | ID=MsG0680030598.01;Name=MsG0680030598.01 |
| Chr6 | mRNA | 5838603 | 5838959 | 5838603 | ID=MsG0680030598.01.T01;Parent=MsG0680030598.01;Name=MsG0680030598.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|0|1|0|118 |
| Chr6 | exon | 5838603 | 5838959 | 5838603 | ID=MsG0680030598.01.T01:exon:5830;Parent=MsG0680030598.01.T01 |
| Chr6 | CDS | 5838603 | 5838959 | 5838603 | ID=MsG0680030598.01.T01:cds;Parent=MsG0680030598.01.T01 |
| Gene Sequence |
| Protein sequence |
