AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030597.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030597.01.T01 MTR_6g007737 80.488 123 5 2 7 114 240 358 7.11e-62 193
MsG0680030597.01.T01 MTR_5g034790 46.903 113 43 3 17 113 229 340 7.86e-25 96.7
MsG0680030597.01.T01 MTR_4g106680 46.721 122 43 5 3 114 191 300 5.48e-21 85.5
MsG0680030597.01.T01 MTR_8g063660 45.455 121 41 3 17 112 185 305 3.39e-20 84.0
MsG0680030597.01.T02 MTR_6g007737 96.875 64 2 0 33 96 240 303 2.69e-38 131
MsG0680030597.01.T02 MTR_5g034790 57.407 54 22 1 43 96 229 281 7.91e-14 65.9
MsG0680030597.01.T02 MTR_4g106680 49.351 77 26 4 24 96 184 251 1.09e-11 59.3
MsG0680030597.01.T03 MTR_6g007737 94.030 67 4 0 1 67 177 243 6.15e-42 139
MsG0680030597.01.T03 MTR_8g063660 71.429 49 14 0 1 49 112 160 5.55e-22 86.3
MsG0680030597.01.T03 MTR_5g034790 76.923 39 8 1 2 39 161 199 2.91e-14 65.5
MsG0680030597.01.T03 MTR_4g106680 62.162 37 14 0 1 37 150 186 2.60e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030597.01.T01 AT5G40250 54.918 122 35 4 10 114 223 341 4.08e-31 113
MsG0680030597.01.T01 AT1G23980 47.115 104 53 1 12 113 225 328 8.19e-21 85.9
MsG0680030597.01.T02 AT5G40250 68.182 66 18 2 36 98 223 288 5.79e-22 88.2
MsG0680030597.01.T03 AT5G40250 75.000 56 13 1 1 55 161 216 1.07e-23 91.7
MsG0680030597.01.T03 AT1G23980 75.000 40 10 0 2 41 163 202 4.40e-14 65.1
MsG0680030597.01.T03 AT3G48030 77.419 31 7 0 1 31 225 255 5.54e-13 62.0
MsG0680030597.01.T03 AT3G48030 44.118 68 30 2 1 62 147 212 2.20e-11 57.0
MsG0680030597.01.T03 AT1G35330 48.214 56 27 1 2 57 148 201 5.04e-11 56.2
MsG0680030597.01.T03 AT2G18650 75.758 33 7 1 2 34 145 176 8.80e-11 55.8

Find 39 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGCCATAACCAAATTT+TGG 0.157592 6:+5837992 None:intergenic
TATTCGATTTCGATGATCTT+AGG 0.158395 6:-5838397 MsG0680030597.01.T02:CDS
TGAAACAAGTAGTAGTAATT+TGG 0.295043 6:-5838218 MsG0680030597.01.T01:CDS
TTGAGGAAGTTGTTGATAAA+GGG 0.310918 6:-5838312 MsG0680030597.01.T01:CDS
GTTGAGGAAGTTGTTGATAA+AGG 0.336638 6:-5838313 MsG0680030597.01.T01:CDS
GTTATCAAAGGAATTGAAAA+TGG 0.356686 6:-5838092 MsG0680030597.01.T01:CDS
TTGAATTTCCCGAGTCGAAC+CGG 0.368984 6:+5838283 None:intergenic
AGAGGAACACTTCAAACTAA+TGG 0.369520 6:-5838440 MsG0680030597.01.T03:CDS
CTATTTCCAAAATTTGGTTA+TGG 0.370519 6:-5837998 MsG0680030597.01.T01:CDS
TGTAATGGAGAGAATGGATT+TGG 0.372483 6:-5838356 MsG0680030597.01.T02:CDS
ATTCGATTTCGATGATCTTA+GGG 0.431362 6:-5838396 MsG0680030597.01.T02:CDS
ATATAAGTTGTATAGATACT+TGG 0.434910 6:-5838493 MsG0680030597.01.T03:CDS
TGGTTGGAAATTCAGAGTTA+AGG 0.444801 6:-5838156 MsG0680030597.01.T01:intron
TGCAAGAAGATGTTATTCAA+TGG 0.469008 6:-5838194 MsG0680030597.01.T01:CDS
AGATCATCGAAATCGAATAT+CGG 0.475880 6:+5838401 None:intergenic
GCAAGAAGATGTTATTCAAT+GGG 0.479763 6:-5838193 MsG0680030597.01.T01:CDS
AATGGGATCTTATCAATATG+TGG 0.489833 6:-5838176 MsG0680030597.01.T01:CDS
TCTTTGTGTTGAGAGTGATT+TGG 0.490017 6:-5838063 MsG0680030597.01.T01:CDS
TGTCCTTGTAATGGAGAGAA+TGG 0.491168 6:-5838362 MsG0680030597.01.T02:CDS
AAGATTAGTAATGTTAGTAA+AGG 0.491990 6:-5838032 MsG0680030597.01.T01:CDS
GGTAGAGAGAATGAAGAAGT+AGG 0.494883 6:-5838241 MsG0680030597.01.T01:CDS
AATTTGGTTATGGCCAAGAA+AGG 0.496237 6:-5837988 MsG0680030597.01.T01:CDS
ATCAAAGGAATTGAAAATGG+TGG 0.506466 6:-5838089 MsG0680030597.01.T01:CDS
GGATCTTATCAATATGTGGT+TGG 0.530819 6:-5838172 MsG0680030597.01.T01:CDS
GATGATCTTAGGGAAGATGA+TGG 0.535942 6:-5838386 MsG0680030597.01.T02:CDS
TGAGGAAGTTGTTGATAAAG+GGG 0.540146 6:-5838311 MsG0680030597.01.T01:CDS
GATGATGGTTGTCCTTGTAA+TGG 0.542547 6:-5838371 MsG0680030597.01.T02:CDS
TCAAAGGAATTGAAAATGGT+GGG 0.548623 6:-5838088 MsG0680030597.01.T01:CDS
AATCCATTCTCTCCATTACA+AGG 0.564226 6:+5838359 None:intergenic
TGAAGTGTTCCTCTACAAAG+AGG 0.570614 6:+5838449 None:intergenic
AGACAAAAGAATTGTTGTTG+AGG 0.584696 6:-5838329 MsG0680030597.01.T01:CDS
GAAATTCAAGAAGACGAATG+TGG 0.607434 6:-5838269 MsG0680030597.01.T01:CDS
CATGATACAAGGGTTATCAA+AGG 0.608541 6:-5838104 MsG0680030597.01.T01:intron
TACAACTTATATGAAAAGCA+TGG 0.613483 6:+5838504 None:intergenic
TGAAAAGCATGGCTACACAT+AGG 0.613953 6:+5838515 None:intergenic
TTGGAAATTCAGAGTTAAGG+TGG 0.621031 6:-5838153 MsG0680030597.01.T01:intron
AAGAAGACGAATGTGGAAGT+CGG 0.638238 6:-5838262 MsG0680030597.01.T01:CDS
TCTACATGTCCTCTTTGTAG+AGG 0.648357 6:-5838458 MsG0680030597.01.T03:CDS
TGTGTTGAGAGTGATTTGGA+AGG 0.653262 6:-5838059 MsG0680030597.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTAGTAATGTTAGTAA+AGG - Chr6:5838445-5838464 MsG0680030597.01.T03:CDS 20.0%
!! ATATAAGTTGTATAGATACT+TGG - Chr6:5837984-5838003 MsG0680030597.01.T01:CDS 20.0%
!!! GTTTTTCTATTTCCAAAATT+TGG - Chr6:5838473-5838492 MsG0680030597.01.T03:CDS 20.0%
! CTATTTCCAAAATTTGGTTA+TGG - Chr6:5838479-5838498 MsG0680030597.01.T03:CDS 25.0%
! GTTATCAAAGGAATTGAAAA+TGG - Chr6:5838385-5838404 MsG0680030597.01.T02:CDS 25.0%
! TACAACTTATATGAAAAGCA+TGG + Chr6:5837976-5837995 None:intergenic 25.0%
! TAGAATTCATCATGATACAA+GGG - Chr6:5838363-5838382 MsG0680030597.01.T02:CDS 25.0%
! TGAAACAAGTAGTAGTAATT+TGG - Chr6:5838259-5838278 MsG0680030597.01.T01:CDS 25.0%
!! AGAAGAAAATTTTCCTTTCT+TGG + Chr6:5838505-5838524 None:intergenic 25.0%
AATGGGATCTTATCAATATG+TGG - Chr6:5838301-5838320 MsG0680030597.01.T01:CDS 30.0%
AATTCTACTCTCATGGTTTA+AGG + Chr6:5838350-5838369 None:intergenic 30.0%
AGATCATCGAAATCGAATAT+CGG + Chr6:5838079-5838098 None:intergenic 30.0%
ATCAAAGGAATTGAAAATGG+TGG - Chr6:5838388-5838407 MsG0680030597.01.T02:CDS 30.0%
ATTCGATTTCGATGATCTTA+GGG - Chr6:5838081-5838100 MsG0680030597.01.T01:CDS 30.0%
GCAAGAAGATGTTATTCAAT+GGG - Chr6:5838284-5838303 MsG0680030597.01.T01:CDS 30.0%
GTAGAATTCATCATGATACA+AGG - Chr6:5838362-5838381 MsG0680030597.01.T02:CDS 30.0%
TATTCGATTTCGATGATCTT+AGG - Chr6:5838080-5838099 MsG0680030597.01.T01:CDS 30.0%
TCAAAGGAATTGAAAATGGT+GGG - Chr6:5838389-5838408 MsG0680030597.01.T02:CDS 30.0%
TGCAAGAAGATGTTATTCAA+TGG - Chr6:5838283-5838302 MsG0680030597.01.T01:CDS 30.0%
! AGACAAAAGAATTGTTGTTG+AGG - Chr6:5838148-5838167 MsG0680030597.01.T01:intron 30.0%
! TTGAGGAAGTTGTTGATAAA+GGG - Chr6:5838165-5838184 MsG0680030597.01.T01:CDS 30.0%
AATCCATTCTCTCCATTACA+AGG + Chr6:5838121-5838140 None:intergenic 35.0%
AATTTGGTTATGGCCAAGAA+AGG - Chr6:5838489-5838508 MsG0680030597.01.T03:CDS 35.0%
AGAGGAACACTTCAAACTAA+TGG - Chr6:5838037-5838056 MsG0680030597.01.T01:CDS 35.0%
CATGATACAAGGGTTATCAA+AGG - Chr6:5838373-5838392 MsG0680030597.01.T02:CDS 35.0%
CATGATGAATTCTACTCTCA+TGG + Chr6:5838357-5838376 None:intergenic 35.0%
GAAATTCAAGAAGACGAATG+TGG - Chr6:5838208-5838227 MsG0680030597.01.T01:CDS 35.0%
GGATCTTATCAATATGTGGT+TGG - Chr6:5838305-5838324 MsG0680030597.01.T01:CDS 35.0%
TCTTTGTGTTGAGAGTGATT+TGG - Chr6:5838414-5838433 MsG0680030597.01.T02:CDS 35.0%
TGGTTGGAAATTCAGAGTTA+AGG - Chr6:5838321-5838340 MsG0680030597.01.T01:CDS 35.0%
TTGGAAATTCAGAGTTAAGG+TGG - Chr6:5838324-5838343 MsG0680030597.01.T01:CDS 35.0%
! GTTGAGGAAGTTGTTGATAA+AGG - Chr6:5838164-5838183 MsG0680030597.01.T01:CDS 35.0%
! TCTTGGCCATAACCAAATTT+TGG + Chr6:5838488-5838507 None:intergenic 35.0%
! TGAGGAAGTTGTTGATAAAG+GGG - Chr6:5838166-5838185 MsG0680030597.01.T01:CDS 35.0%
! TGTAATGGAGAGAATGGATT+TGG - Chr6:5838121-5838140 MsG0680030597.01.T01:intron 35.0%
AAGAAGACGAATGTGGAAGT+CGG - Chr6:5838215-5838234 MsG0680030597.01.T01:CDS 40.0%
GATGATCTTAGGGAAGATGA+TGG - Chr6:5838091-5838110 MsG0680030597.01.T01:CDS 40.0%
GGTAGAGAGAATGAAGAAGT+AGG - Chr6:5838236-5838255 MsG0680030597.01.T01:CDS 40.0%
TCTACATGTCCTCTTTGTAG+AGG - Chr6:5838019-5838038 MsG0680030597.01.T01:CDS 40.0%
! GATGATGGTTGTCCTTGTAA+TGG - Chr6:5838106-5838125 MsG0680030597.01.T01:intron 40.0%
! TGAAGTGTTCCTCTACAAAG+AGG + Chr6:5838031-5838050 None:intergenic 40.0%
! TGTCCTTGTAATGGAGAGAA+TGG - Chr6:5838115-5838134 MsG0680030597.01.T01:intron 40.0%
! TGTGTTGAGAGTGATTTGGA+AGG - Chr6:5838418-5838437 MsG0680030597.01.T02:CDS 40.0%
!! TGTTGATAAAGGGGTTTTGC+CGG - Chr6:5838175-5838194 MsG0680030597.01.T01:CDS 40.0%
TTGAATTTCCCGAGTCGAAC+CGG + Chr6:5838197-5838216 None:intergenic 45.0%
!!! GGGGTTTTGCCGGTTCGACT+CGG - Chr6:5838185-5838204 MsG0680030597.01.T01:CDS 60.0%
!!! GGGTTTTGCCGGTTCGACTC+GGG - Chr6:5838186-5838205 MsG0680030597.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 5837965 5838534 5837965 ID=MsG0680030597.01;Name=MsG0680030597.01
Chr6 mRNA 5837965 5838365 5837965 ID=MsG0680030597.01.T01;Parent=MsG0680030597.01;Name=MsG0680030597.01.T01;_AED=0.41;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|118
Chr6 exon 5837965 5838118 5837965 ID=MsG0680030597.01.T01:exon:5823;Parent=MsG0680030597.01.T01
Chr6 exon 5838163 5838365 5838163 ID=MsG0680030597.01.T01:exon:5822;Parent=MsG0680030597.01.T01
Chr6 CDS 5838163 5838365 5838163 ID=MsG0680030597.01.T01:cds;Parent=MsG0680030597.01.T01
Chr6 CDS 5837965 5838118 5837965 ID=MsG0680030597.01.T01:cds;Parent=MsG0680030597.01.T01
Chr6 mRNA 5838147 5838443 5838147 ID=MsG0680030597.01.T02;Parent=MsG0680030597.01;Name=MsG0680030597.01.T02;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|98
Chr6 exon 5838147 5838443 5838147 ID=MsG0680030597.01.T02:exon:5824;Parent=MsG0680030597.01.T02
Chr6 CDS 5838147 5838443 5838147 ID=MsG0680030597.01.T02:cds;Parent=MsG0680030597.01.T02
Chr6 mRNA 5838331 5838534 5838331 ID=MsG0680030597.01.T03;Parent=MsG0680030597.01;Name=MsG0680030597.01.T03;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|67
Chr6 exon 5838331 5838534 5838331 ID=MsG0680030597.01.T03:exon:5825;Parent=MsG0680030597.01.T03
Chr6 CDS 5838331 5838534 5838331 ID=MsG0680030597.01.T03:cds;Parent=MsG0680030597.01.T03
Gene Sequence

>MsG0680030597.01.T01

ATGGATTTGGTACTAGACAAAAGAATTGTTGTTGAGGAAGTTGTTGATAAAGGGGTTTTGCCGGTTCGACTCGGGAAATTCAAGAAGACGAATGTGGAAGTCGGTAGAGAGAATGAAGAAGTAGGTGAAACAAGTAGTAGTAATTTGGATGCAAGAAGATGTTATTCAATGGGATCTTATCAATATGTGGTTGGAAATTCAGAAAGGGTTATCAAAGGAATTGAAAATGGTGGGAATCTTTGTGTTGAGAGTGATTTGGAAGGAAAGAAGATTAGTAATGTTAGTAAAGGTGAAAGTTTTTCTATTTCCAAAATTTGGTTATGGCCAAGAAAGGAAAATTTTCTTCTAGTTCTTTAG

>MsG0680030597.01.T02

ATGGATTTTCTATTGAAAATCCGATATTCGATTTCGATGATCTTAGGGAAGATGATGGTTGTCCTTGTAATGGAGAGAATGGATTTGGTACTAGACAAAAGAATTGTTGTTGAGGAAGTTGTTGATAAAGGGGTTTTGCCGGTTCGACTCGGGAAATTCAAGAAGACGAATGTGGAAGTCGGTAGAGAGAATGAAGAAGTAGGTGAAACAAGTAGTAGTAATTTGGATGCAAGAAGATGTTATTCAATGGGATCTTATCAATATGTGGTTGGAAATTCAGAGTTAAGGTGGCCTTAA

>MsG0680030597.01.T03

ATGTGTAGCCATGCTTTTCATATAAGTTGTATAGATACTTGGCTACTTTCAAACTCTACATGTCCTCTTTGTAGAGGAACACTTCAAACTAATGGATTTTCTATTGAAAATCCGATATTCGATTTCGATGATCTTAGGGAAGATGATGGTTGTCCTTGTAATGGAGAGAATGGATTTGGTACTAGACAAAAGAATTGTTGTTGA

Protein sequence

>MsG0680030597.01.T01

MDLVLDKRIVVEEVVDKGVLPVRLGKFKKTNVEVGRENEEVGETSSSNLDARRCYSMGSYQYVVGNSERVIKGIENGGNLCVESDLEGKKISNVSKGESFSISKIWLWPRKENFLLVL*

>MsG0680030597.01.T02

MDFLLKIRYSISMILGKMMVVLVMERMDLVLDKRIVVEEVVDKGVLPVRLGKFKKTNVEVGRENEEVGETSSSNLDARRCYSMGSYQYVVGNSELRWP*

>MsG0680030597.01.T03

MCSHAFHISCIDTWLLSNSTCPLCRGTLQTNGFSIENPIFDFDDLREDDGCPCNGENGFGTRQKNCC*