Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030654.01.T01 | XP_003618370.2 | 81.982 | 111 | 0 | 1 | 1 | 111 | 1 | 91 | 7.61E-48 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030654.01.T01 | Q9LFR1 | 55.963 | 109 | 28 | 1 | 1 | 109 | 1 | 89 | 6.13E-30 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030654.01.T01 | G7KJG0 | 81.982 | 111 | 0 | 1 | 1 | 111 | 1 | 91 | 3.64e-48 | 171 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021690.01 | MsG0680030654.01 | 0.808637 | 2.807807e-50 | 1.613738e-47 |
| MsG0680030652.01 | MsG0680030654.01 | 0.830218 | 3.323413e-55 | 3.479436e-52 |
| MsG0680030653.01 | MsG0680030654.01 | 0.854640 | 1.075917e-61 | 2.418976e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030654.01.T01 | MTR_6g008690 | 81.982 | 111 | 0 | 1 | 1 | 111 | 1 | 91 | 9.22e-52 | 171 |
| MsG0680030654.01.T01 | MTR_8g038880 | 47.059 | 68 | 36 | 0 | 44 | 111 | 37 | 104 | 3.63e-15 | 70.9 |
| MsG0680030654.01.T01 | MTR_4g033375 | 42.353 | 85 | 48 | 1 | 44 | 127 | 49 | 133 | 1.11e-13 | 66.6 |
| MsG0680030654.01.T01 | MTR_7g065080 | 45.588 | 68 | 37 | 0 | 44 | 111 | 48 | 115 | 2.15e-13 | 65.9 |
| MsG0680030654.01.T01 | MTR_4g033395 | 41.176 | 85 | 47 | 2 | 44 | 125 | 49 | 133 | 4.00e-13 | 65.1 |
| MsG0680030654.01.T01 | MTR_3g005010 | 44.776 | 67 | 37 | 0 | 44 | 110 | 47 | 113 | 1.40e-12 | 63.5 |
| MsG0680030654.01.T01 | MTR_3g069500 | 42.857 | 77 | 44 | 0 | 35 | 111 | 43 | 119 | 1.70e-12 | 63.5 |
| MsG0680030654.01.T01 | MTR_3g069420 | 40.000 | 80 | 44 | 1 | 31 | 110 | 42 | 117 | 1.85e-12 | 63.2 |
| MsG0680030654.01.T01 | MTR_4g015080 | 48.529 | 68 | 35 | 0 | 44 | 111 | 51 | 118 | 2.02e-12 | 63.2 |
| MsG0680030654.01.T01 | MTR_3g014790 | 41.558 | 77 | 45 | 0 | 35 | 111 | 25 | 101 | 2.06e-12 | 63.2 |
| MsG0680030654.01.T01 | MTR_7g067010 | 45.588 | 68 | 37 | 0 | 44 | 111 | 41 | 108 | 1.31e-11 | 60.8 |
| MsG0680030654.01.T01 | MTR_4g019580 | 37.805 | 82 | 50 | 1 | 44 | 124 | 53 | 134 | 1.71e-11 | 60.5 |
| MsG0680030654.01.T01 | MTR_7g098150 | 44.118 | 68 | 38 | 0 | 44 | 111 | 50 | 117 | 2.15e-11 | 60.1 |
| MsG0680030654.01.T01 | MTR_3g072300 | 47.059 | 68 | 36 | 0 | 44 | 111 | 41 | 108 | 4.15e-11 | 59.3 |
| MsG0680030654.01.T01 | MTR_7g098230 | 40.698 | 86 | 51 | 0 | 24 | 109 | 23 | 108 | 5.95e-11 | 58.9 |
| MsG0680030654.01.T01 | MTR_3g069440 | 44.444 | 72 | 40 | 0 | 35 | 106 | 41 | 112 | 6.48e-11 | 58.9 |
| MsG0680030654.01.T01 | MTR_1g041835 | 36.170 | 94 | 55 | 2 | 32 | 124 | 36 | 125 | 7.33e-11 | 58.5 |
| MsG0680030654.01.T01 | MTR_4g033330 | 42.857 | 63 | 36 | 0 | 44 | 106 | 48 | 110 | 7.67e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030654.01.T01 | AT5G14940 | 55.963 | 109 | 28 | 1 | 1 | 109 | 1 | 89 | 6.25e-31 | 115 |
| MsG0680030654.01.T01 | AT3G01350 | 56.190 | 105 | 26 | 1 | 5 | 109 | 5 | 89 | 1.44e-30 | 114 |
| MsG0680030654.01.T01 | AT3G54450 | 41.558 | 77 | 45 | 0 | 35 | 111 | 20 | 96 | 7.04e-15 | 70.1 |
| MsG0680030654.01.T01 | AT1G22550 | 52.381 | 63 | 30 | 0 | 44 | 106 | 47 | 109 | 2.66e-13 | 65.9 |
| MsG0680030654.01.T01 | AT2G02020 | 48.485 | 66 | 34 | 0 | 44 | 109 | 59 | 124 | 4.53e-13 | 65.1 |
| MsG0680030654.01.T01 | AT2G02020 | 48.485 | 66 | 34 | 0 | 44 | 109 | 59 | 124 | 4.78e-13 | 65.1 |
| MsG0680030654.01.T01 | AT1G22570 | 50.794 | 63 | 31 | 0 | 44 | 106 | 10 | 72 | 8.50e-13 | 64.3 |
| MsG0680030654.01.T01 | AT1G22570 | 50.794 | 63 | 31 | 0 | 44 | 106 | 47 | 109 | 1.04e-12 | 63.9 |
| MsG0680030654.01.T01 | AT1G72140 | 38.554 | 83 | 50 | 1 | 44 | 125 | 51 | 133 | 1.13e-12 | 63.9 |
| MsG0680030654.01.T01 | AT2G37900 | 41.558 | 77 | 45 | 0 | 35 | 111 | 46 | 122 | 3.00e-12 | 62.8 |
| MsG0680030654.01.T01 | AT2G02040 | 46.970 | 66 | 35 | 0 | 44 | 109 | 58 | 123 | 4.18e-12 | 62.4 |
| MsG0680030654.01.T01 | AT1G62200 | 50.000 | 66 | 33 | 0 | 44 | 109 | 69 | 134 | 7.11e-12 | 61.6 |
| MsG0680030654.01.T01 | AT1G62200 | 50.000 | 66 | 33 | 0 | 44 | 109 | 69 | 134 | 7.27e-12 | 61.6 |
| MsG0680030654.01.T01 | AT1G62200 | 50.000 | 66 | 33 | 0 | 44 | 109 | 83 | 148 | 7.48e-12 | 61.6 |
| MsG0680030654.01.T01 | AT1G22540 | 42.169 | 83 | 47 | 1 | 44 | 125 | 47 | 129 | 2.54e-11 | 60.1 |
| MsG0680030654.01.T01 | AT3G53960 | 39.706 | 68 | 41 | 0 | 44 | 111 | 54 | 121 | 4.45e-11 | 59.3 |
| MsG0680030654.01.T01 | AT1G72125 | 48.529 | 68 | 35 | 0 | 44 | 111 | 46 | 113 | 6.49e-11 | 58.9 |
| MsG0680030654.01.T01 | AT3G54140 | 44.776 | 67 | 37 | 0 | 44 | 110 | 41 | 107 | 6.96e-11 | 58.9 |
| MsG0680030654.01.T01 | AT3G54140 | 44.776 | 67 | 37 | 0 | 44 | 110 | 41 | 107 | 6.96e-11 | 58.9 |
Find 29 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCTATTGCTGATGCTTAT+TGG | 0.205664 | 6:-6642640 | MsG0680030654.01.T01:CDS |
| CAAAGGGGTAGCATCAAATT+TGG | 0.248336 | 6:-6642762 | MsG0680030654.01.T01:CDS |
| ACCTATTGCTGATGCTTATT+GGG | 0.269588 | 6:-6642639 | MsG0680030654.01.T01:CDS |
| GAGAAAAGATGAGGACATTA+TGG | 0.364757 | 6:+6642604 | None:intergenic |
| TGCCACCAACAATGGCATTA+TGG | 0.367205 | 6:+6642661 | None:intergenic |
| CTCTTTGAAACAGTCATTGC+AGG | 0.374811 | 6:-6642803 | MsG0680030654.01.T01:CDS |
| CACTTCCATAATGCCATTGT+TGG | 0.382096 | 6:-6642666 | MsG0680030654.01.T01:CDS |
| CAGCAAAGATGGTTAATAGT+TGG | 0.392002 | 6:-6642697 | MsG0680030654.01.T01:CDS |
| TGGTATCTTCTTATAATGGC+TGG | 0.414363 | 6:-6643488 | None:intergenic |
| AGCAAAGATGGTTAATAGTT+GGG | 0.441043 | 6:-6642696 | MsG0680030654.01.T01:CDS |
| TGTAAATATGTAAATGCAAA+AGG | 0.443995 | 6:+6642538 | None:intergenic |
| CAACTCATCTGCAGCAAAGA+TGG | 0.448211 | 6:-6642708 | MsG0680030654.01.T01:CDS |
| AATTCATGCATTCTCCTCAT+AGG | 0.453128 | 6:-6643440 | MsG0680030654.01.T01:CDS |
| GCCCAATAAGCATCAGCAAT+AGG | 0.462037 | 6:+6642638 | None:intergenic |
| TAGAGAGGTTTGCATTCAAA+GGG | 0.464940 | 6:-6642778 | MsG0680030654.01.T01:CDS |
| GCATGAATTGCTGAGCCTCT+TGG | 0.476082 | 6:+6643454 | None:intergenic |
| AAGATGGTTAATAGTTGGGT+TGG | 0.477748 | 6:-6642692 | MsG0680030654.01.T01:CDS |
| TTCCATAATGCCATTGTTGG+TGG | 0.499004 | 6:-6642663 | MsG0680030654.01.T01:CDS |
| ATAGAGAGGTTTGCATTCAA+AGG | 0.511614 | 6:-6642779 | MsG0680030654.01.T01:CDS |
| AACTTATCTAACTGATGTTG+TGG | 0.535055 | 6:-6642738 | MsG0680030654.01.T01:CDS |
| AATATGTAAATGCAAAAGGA+AGG | 0.549925 | 6:+6642542 | None:intergenic |
| ACACATAGAATTTACCTATG+AGG | 0.562483 | 6:+6643426 | None:intergenic |
| AAAAGATGAGGACATTATGG+TGG | 0.581494 | 6:+6642607 | None:intergenic |
| TATCTTCTTATAATGGCTGG+TGG | 0.599636 | 6:-6643485 | None:intergenic |
| CAGTCATTGCAGGTATAGAG+AGG | 0.605527 | 6:-6642793 | MsG0680030654.01.T01:CDS |
| GCAATAGGTGCCACCAACAA+TGG | 0.628514 | 6:+6642653 | None:intergenic |
| CATAACATAGAGAAAAGATG+AGG | 0.677023 | 6:+6642595 | None:intergenic |
| AGAGAGGTTTGCATTCAAAG+GGG | 0.708268 | 6:-6642777 | MsG0680030654.01.T01:CDS |
| CTGGTGGACAGAAAACCAAG+AGG | 0.752478 | 6:-6643469 | MsG0680030654.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAATATCATAAATCAAA+AGG | + | Chr6:6643134-6643153 | None:intergenic | 15.0% |
| !! | ATTCTATTATATCATTCTTA+GGG | - | Chr6:6643147-6643166 | MsG0680030654.01.T01:intron | 15.0% |
| !! | TAGAATATCATAAATCAAAA+GGG | + | Chr6:6643133-6643152 | None:intergenic | 15.0% |
| !! | TATTCTATTATATCATTCTT+AGG | - | Chr6:6643146-6643165 | MsG0680030654.01.T01:intron | 15.0% |
| !! | AGCAAATTTCATATTATCAA+TGG | + | Chr6:6642788-6642807 | None:intergenic | 20.0% |
| !! | ATGCAAGAATAATAGAATAT+TGG | + | Chr6:6643109-6643128 | None:intergenic | 20.0% |
| !! | TGTAAATATGTAAATGCAAA+AGG | + | Chr6:6643470-6643489 | None:intergenic | 20.0% |
| !! | TTAGAGAAAAATATCACAAA+TGG | + | Chr6:6642733-6642752 | None:intergenic | 20.0% |
| !!! | TTCAAGTTTCAAACTTATTA+GGG | - | Chr6:6642642-6642661 | MsG0680030654.01.T01:CDS | 20.0% |
| !!! | TTTCAAGTTTCAAACTTATT+AGG | - | Chr6:6642641-6642660 | MsG0680030654.01.T01:CDS | 20.0% |
| ! | AATATGTAAATGCAAAAGGA+AGG | + | Chr6:6643466-6643485 | None:intergenic | 25.0% |
| ! | AATTCAAATCCATTCAATTG+CGG | + | Chr6:6642910-6642929 | None:intergenic | 25.0% |
| ! | TCATATTATCAATGGAATTG+AGG | + | Chr6:6642780-6642799 | None:intergenic | 25.0% |
| !! | GAAGTATATAATTTTGTGGA+AGG | + | Chr6:6643444-6643463 | None:intergenic | 25.0% |
| !!! | AGAGTTTTAAATCATGGTAA+TGG | + | Chr6:6643060-6643079 | None:intergenic | 25.0% |
| !!! | ATAGGAAACTAGTCTTTAAT+AGG | - | Chr6:6642818-6642837 | MsG0680030654.01.T01:CDS | 25.0% |
| !!! | GAAGGAAGTATATAATTTTG+TGG | + | Chr6:6643448-6643467 | None:intergenic | 25.0% |
| !!! | TAGGAAACTAGTCTTTAATA+GGG | - | Chr6:6642819-6642838 | MsG0680030654.01.T01:CDS | 25.0% |
| !!! | TCTTCTAGAGTTTTAAATCA+TGG | + | Chr6:6643066-6643085 | None:intergenic | 25.0% |
| AACTTATCTAACTGATGTTG+TGG | - | Chr6:6643267-6643286 | MsG0680030654.01.T01:intron | 30.0% | |
| ACACATAGAATTTACCTATG+AGG | + | Chr6:6642582-6642601 | None:intergenic | 30.0% | |
| AGATTGATGCAAGTTCAATA+GGG | + | Chr6:6642994-6643013 | None:intergenic | 30.0% | |
| AGCAAAGATGGTTAATAGTT+GGG | - | Chr6:6643309-6643328 | MsG0680030654.01.T01:intron | 30.0% | |
| CATAACATAGAGAAAAGATG+AGG | + | Chr6:6643413-6643432 | None:intergenic | 30.0% | |
| CTGTAGTTGAATTTAACTGT+AGG | - | Chr6:6642958-6642977 | MsG0680030654.01.T01:intron | 30.0% | |
| ! | ATTTTCGTCTTCCACAATAT+CGG | - | Chr6:6642845-6642864 | MsG0680030654.01.T01:intron | 30.0% |
| ! | CTCATCTTTTCTCTATGTTA+TGG | - | Chr6:6643411-6643430 | MsG0680030654.01.T01:intron | 30.0% |
| ! | TTTGAGTTAAAGCATATGCT+TGG | - | Chr6:6642697-6642716 | MsG0680030654.01.T01:CDS | 30.0% |
| !! | AATTTGCTGCTGAAAAACAT+AGG | - | Chr6:6642800-6642819 | MsG0680030654.01.T01:CDS | 30.0% |
| AAAAGATGAGGACATTATGG+TGG | + | Chr6:6643401-6643420 | None:intergenic | 35.0% | |
| AAGATGGTTAATAGTTGGGT+TGG | - | Chr6:6643313-6643332 | MsG0680030654.01.T01:intron | 35.0% | |
| AATGGAATTGAGGCTTAAGA+AGG | + | Chr6:6642770-6642789 | None:intergenic | 35.0% | |
| AATTCATGCATTCTCCTCAT+AGG | - | Chr6:6642565-6642584 | MsG0680030654.01.T01:CDS | 35.0% | |
| AATTTAACTGTAGGCAATCC+TGG | - | Chr6:6642967-6642986 | MsG0680030654.01.T01:intron | 35.0% | |
| ACCTATTGCTGATGCTTATT+GGG | - | Chr6:6643366-6643385 | MsG0680030654.01.T01:intron | 35.0% | |
| ATAGAGAGGTTTGCATTCAA+AGG | - | Chr6:6643226-6643245 | MsG0680030654.01.T01:intron | 35.0% | |
| ATCATCATACCGCAATTGAA+TGG | - | Chr6:6642898-6642917 | MsG0680030654.01.T01:intron | 35.0% | |
| CAGCAAAGATGGTTAATAGT+TGG | - | Chr6:6643308-6643327 | MsG0680030654.01.T01:intron | 35.0% | |
| CTAGATATTAAGCAGAATCG+CGG | + | Chr6:6643024-6643043 | None:intergenic | 35.0% | |
| GAGAAAAGATGAGGACATTA+TGG | + | Chr6:6643404-6643423 | None:intergenic | 35.0% | |
| GAGATTGATGCAAGTTCAAT+AGG | + | Chr6:6642995-6643014 | None:intergenic | 35.0% | |
| TAGAGAGGTTTGCATTCAAA+GGG | - | Chr6:6643227-6643246 | MsG0680030654.01.T01:intron | 35.0% | |
| TTGTAGTTGAATTCAACTGC+AGG | + | Chr6:6642934-6642953 | None:intergenic | 35.0% | |
| AGAGAGGTTTGCATTCAAAG+GGG | - | Chr6:6643228-6643247 | MsG0680030654.01.T01:intron | 40.0% | |
| CAAAGGGGTAGCATCAAATT+TGG | - | Chr6:6643243-6643262 | MsG0680030654.01.T01:intron | 40.0% | |
| CACCTATTGCTGATGCTTAT+TGG | - | Chr6:6643365-6643384 | MsG0680030654.01.T01:intron | 40.0% | |
| CACTTCCATAATGCCATTGT+TGG | - | Chr6:6643339-6643358 | MsG0680030654.01.T01:intron | 40.0% | |
| TAATGTCGAAGCCGATATTG+TGG | + | Chr6:6642859-6642878 | None:intergenic | 40.0% | |
| TTCCATAATGCCATTGTTGG+TGG | - | Chr6:6643342-6643361 | MsG0680030654.01.T01:intron | 40.0% | |
| TTGCTCATGATTGAGCACAA+TGG | - | Chr6:6642672-6642691 | MsG0680030654.01.T01:CDS | 40.0% | |
| !! | CAATATCGGCTTCGACATTA+TGG | - | Chr6:6642859-6642878 | MsG0680030654.01.T01:intron | 40.0% |
| !! | CTCTTTGAAACAGTCATTGC+AGG | - | Chr6:6643202-6643221 | MsG0680030654.01.T01:intron | 40.0% |
| ATGCAAGTTCAATAGGGACC+AGG | + | Chr6:6642988-6643007 | None:intergenic | 45.0% | |
| CAACTCATCTGCAGCAAAGA+TGG | - | Chr6:6643297-6643316 | MsG0680030654.01.T01:intron | 45.0% | |
| CAGTCATTGCAGGTATAGAG+AGG | - | Chr6:6643212-6643231 | MsG0680030654.01.T01:intron | 45.0% | |
| GCCCAATAAGCATCAGCAAT+AGG | + | Chr6:6643370-6643389 | None:intergenic | 45.0% | |
| TGCCACCAACAATGGCATTA+TGG | + | Chr6:6643347-6643366 | None:intergenic | 45.0% | |
| GCATGAATTGCTGAGCCTCT+TGG | + | Chr6:6642554-6642573 | None:intergenic | 50.0% | |
| ! | CTGGTGGACAGAAAACCAAG+AGG | - | Chr6:6642536-6642555 | MsG0680030654.01.T01:CDS | 50.0% |
| ! | GCAATAGGTGCCACCAACAA+TGG | + | Chr6:6643355-6643374 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 6642532 | 6643495 | 6642532 | ID=MsG0680030654.01;Name=MsG0680030654.01 |
| Chr6 | mRNA | 6642532 | 6643495 | 6642532 | ID=MsG0680030654.01.T01;Parent=MsG0680030654.01;Name=MsG0680030654.01.T01;_AED=0.46;_eAED=0.53;_QI=0|0|0|1|0|0|2|0|131 |
| Chr6 | exon | 6643428 | 6643495 | 6643428 | ID=MsG0680030654.01.T01:exon:6288;Parent=MsG0680030654.01.T01 |
| Chr6 | exon | 6642532 | 6642859 | 6642532 | ID=MsG0680030654.01.T01:exon:6287;Parent=MsG0680030654.01.T01 |
| Chr6 | CDS | 6643428 | 6643495 | 6643428 | ID=MsG0680030654.01.T01:cds;Parent=MsG0680030654.01.T01 |
| Chr6 | CDS | 6642532 | 6642859 | 6642532 | ID=MsG0680030654.01.T01:cds;Parent=MsG0680030654.01.T01 |
| Gene Sequence |
| Protein sequence |
