AlfalfaGEDB | MsG0680030789.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030789.01

KEGG: K22285
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030789.01.T01	GAU37610.1	86.17	94	13	0	5	98	124	217	1.34E-48	164

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030789.01.T01	Q9SA38	55.294	85	38	0	5	89	416	500	1.05E-26	105

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030789.01.T01	A0A2Z6NM41	86.170	94	13	0	5	98	124	217	6.41e-49	164

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280008362.01	MsG0680030789.01	0.818127	2.304850e-52	1.710539e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030789.01.T01	MTR_5g080050	88.298	94	11	0	5	98	417	510	1.31e-51	169
MsG0680030789.01.T01	MTR_8g039190	85.106	94	14	0	5	98	414	507	6.71e-49	162
MsG0680030789.01.T01	MTR_8g039070	85.106	94	14	0	5	98	378	471	1.01e-48	161
MsG0680030789.01.T01	MTR_5g082760	79.787	94	19	0	5	98	427	520	2.33e-45	153
MsG0680030789.01.T01	MTR_4g030210	72.917	96	26	0	6	101	416	511	2.05e-40	139
MsG0680030789.01.T01	MTR_4g030040	70.000	100	28	1	6	105	411	508	7.58e-40	138
MsG0680030789.01.T01	MTR_4g030040	70.000	100	28	1	6	105	412	509	8.96e-40	138
MsG0680030789.01.T01	MTR_8g447340	46.000	100	52	1	5	104	424	521	3.89e-24	95.5

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030789.01.T01	AT1G16390	55.294	85	38	5	89	416	500	1.07e-27	105
MsG0680030789.01.T01	AT1G79360	47.674	86	45	4	89	420	505	2.52e-24	95.9
MsG0680030789.01.T01	AT1G79410	40.000	95	57	6	100	413	507	1.25e-17	77.0
MsG0680030789.01.T01	AT1G73220	42.857	84	48	5	88	435	518	4.48e-16	72.4
MsG0680030789.01.T01	AT1G16370	36.170	94	60	7	100	420	513	5.97e-16	72.4

Find 14 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTAGGATCATTTACCTAAT+TGG	0.181285	6:-9397384	None:intergenic
TTCTTTGTATTCAACATTCT+TGG	0.209029	6:+9397191	MsG0680030789.01.T01:CDS
CAAACACAGCAATGTGAAAT+TGG	0.272144	6:-9397149	None:intergenic
CCTCCCAGCAGATATCAAAA+AGG	0.322011	6:-9397077	None:intergenic
AGAGAAAAGAAAGTCCAATT+AGG	0.360062	6:+9397370	MsG0680030789.01.T01:three_prime_UTR
AGAACAATCCCTTGAGTCTC+TGG	0.373429	6:-9397172	None:intergenic
GTGAGACAAGTGATTGTGTT+TGG	0.457373	6:+9397040	MsG0680030789.01.T01:CDS
CATAAAAGAAATGCAGATCA+TGG	0.459850	6:+9397250	MsG0680030789.01.T01:three_prime_UTR
GAGGAACACAACAACTTCAT+TGG	0.498734	6:+9397018	MsG0680030789.01.T01:CDS
CTGTGTTTGCCAGAGACTCA+AGG	0.534894	6:+9397163	MsG0680030789.01.T01:CDS
TGTGTTTGCCAGAGACTCAA+GGG	0.566286	6:+9397164	MsG0680030789.01.T01:CDS
AGTTGTTTCCGACATGTGTG+AGG	0.640373	6:+9396999	MsG0680030789.01.T01:CDS
GTTGTGTTCCTCACACATGT+CGG	0.642208	6:-9397007	None:intergenic
CATTCTTGGACAAACAAGAG+AGG	0.683134	6:+9397205	MsG0680030789.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAATAATATTTTCTCTTA+TGG	+	Chr6:9397100-9397119	MsG0680030789.01.T01:CDS	10.0%
!	TTCTTTGTATTCAACATTCT+TGG	+	Chr6:9397191-9397210	MsG0680030789.01.T01:CDS	25.0%
	AGAGAAAAGAAAGTCCAATT+AGG	+	Chr6:9397370-9397389	MsG0680030789.01.T01:three_prime_UTR	30.0%
	CATAAAAGAAATGCAGATCA+TGG	+	Chr6:9397250-9397269	MsG0680030789.01.T01:three_prime_UTR	30.0%
!	ATTTTCTCTTATGGTGTGTT+TGG	+	Chr6:9397109-9397128	MsG0680030789.01.T01:CDS	30.0%
	CAAACACAGCAATGTGAAAT+TGG	-	Chr6:9397152-9397171	None:intergenic	35.0%
	CATTCTTGGACAAACAAGAG+AGG	+	Chr6:9397205-9397224	MsG0680030789.01.T01:CDS	40.0%
	CTCCCAGCAGATATCAAAAA+GGG	-	Chr6:9397079-9397098	None:intergenic	40.0%
	GAGGAACACAACAACTTCAT+TGG	+	Chr6:9397018-9397037	MsG0680030789.01.T01:CDS	40.0%
	GTGAGACAAGTGATTGTGTT+TGG	+	Chr6:9397040-9397059	MsG0680030789.01.T01:CDS	40.0%
!	GTCCCTTTTTGATATCTGCT+GGG	+	Chr6:9397074-9397093	MsG0680030789.01.T01:CDS	40.0%
!	TGTCCCTTTTTGATATCTGC+TGG	+	Chr6:9397073-9397092	MsG0680030789.01.T01:CDS	40.0%
!!	GTTTGGTTCCTTGCGTTTTT+CGG	+	Chr6:9396935-9396954	MsG0680030789.01.T01:CDS	40.0%
	AGAACAATCCCTTGAGTCTC+TGG	-	Chr6:9397175-9397194	None:intergenic	45.0%
	CCTCCCAGCAGATATCAAAA+AGG	-	Chr6:9397080-9397099	None:intergenic	45.0%
	GTTGTGTTCCTCACACATGT+CGG	-	Chr6:9397010-9397029	None:intergenic	45.0%
	TGTGTTTGCCAGAGACTCAA+GGG	+	Chr6:9397164-9397183	MsG0680030789.01.T01:CDS	45.0%
!	AGTTGTTTCCGACATGTGTG+AGG	+	Chr6:9396999-9397018	MsG0680030789.01.T01:CDS	45.0%
!	CCTTTTTGATATCTGCTGGG+AGG	+	Chr6:9397077-9397096	MsG0680030789.01.T01:CDS	45.0%
	CTGTGTTTGCCAGAGACTCA+AGG	+	Chr6:9397163-9397182	MsG0680030789.01.T01:CDS	50.0%
!!	TACAAGCGCCGAAAAACGCA+AGG	-	Chr6:9396946-9396965	None:intergenic	50.0%
!!	TGCGTTTTTCGGCGCTTGTA+CGG	+	Chr6:9396946-9396965	MsG0680030789.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	9396932	9397397	9396932	ID=MsG0680030789.01;Name=MsG0680030789.01
Chr6	mRNA	9396932	9397397	9396932	ID=MsG0680030789.01.T01;Parent=MsG0680030789.01;Name=MsG0680030789.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|148\|105
Chr6	exon	9396932	9397397	9396932	ID=MsG0680030789.01.T01:exon:7501;Parent=MsG0680030789.01.T01
Chr6	CDS	9396932	9397249	9396932	ID=MsG0680030789.01.T01:cds;Parent=MsG0680030789.01.T01
Chr6	three_prime_UTR	9397250	9397397	9397250	ID=MsG0680030789.01.T01:three_prime_utr;Parent=MsG0680030789.01.T01
Chr6	mRNA	9397012	9397107	9397012	ID=MsG0680030789.01.T02;Parent=MsG0680030789.01;Name=MsG0680030789.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|31
Chr6	exon	9397012	9397107	9397012	ID=MsG0680030789.01.T02:exon:7502;Parent=MsG0680030789.01.T02
Chr6	CDS	9397012	9397107	9397012	ID=MsG0680030789.01.T02:cds;Parent=MsG0680030789.01.T02

Gene Sequence

>MsG0680030789.01.T02

ATGTGTGAGGAACACAACAACTTCATTGGTGAGACAAGTGATTGTGTTTGGTTGCATATTCTGTCCCTTTTTGATATCTGCTGGGAGGAAAAATAA

>MsG0680030789.01.T01

ATGGTTTGGTTCCTTGCGTTTTTCGGCGCTTGTACGGCTTATAATGTGTTTCTAATATACATTATAGAGTTGTTTCCGACATGTGTGAGGAACACAACAACTTCATTGGTGAGACAAGTGATTGTGTTTGGTTGCATATTCTGTCCCTTTTTGATATCTGCTGGGAGGAAAAATAATATTTTCTCTTATGGTGTGTTTGGAGTTGTTATCATGTTGTCCAATTTCACATTGCTGTGTTTGCCAGAGACTCAAGGGATTGTTCTTTGTATTCAACATTCTTGGACAAACAAGAGAGGTCATGACTCATTAAGTATATGA

Protein sequence

>MsG0680030789.01.T02

MCEEHNNFIGETSDCVWLHILSLFDICWEEK*

>MsG0680030789.01.T01

MVWFLAFFGACTAYNVFLIYIIELFPTCVRNTTTSLVRQVIVFGCIFCPFLISAGRKNNIFSYGVFGVVIMLSNFTLLCLPETQGIVLCIQHSWTNKRGHDSLSI*