AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031265.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031265.01.T01 MTR_3g115080 42.593 108 56 3 1 102 1 108 1.51e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031265.01.T01 AT1G20460 42.453 106 57 3 1 102 1 106 1.14e-14 64.3
MsG0680031265.01.T01 AT1G76185 42.453 106 57 3 1 102 1 106 4.57e-12 57.8

Find 44 sgRNAs with CRISPR-Local

Find 319 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTGTTAGTTAGTTAAAA+AGG 0.180464 6:+17013938 MsG0680031265.01.T01:five_prime_UTR
CGAGTGTTACAAGTAAAATA+AGG 0.239823 6:-17017940 None:intergenic
TATATCTTACATAATTAATT+TGG 0.249603 6:+17017790 MsG0680031265.01.T01:three_prime_UTR
ACTTGTAACACTCGTAAAAT+TGG 0.284676 6:+17017949 MsG0680031265.01.T01:three_prime_UTR
ATTCACTTAGCTTGTGCATA+TGG 0.310277 6:-17017908 None:intergenic
AGCAGAAGATCCTGGGAAAA+AGG 0.316556 6:-17018032 None:intergenic
TCATACGCCTAAGAGCTTCT+AGG 0.326975 6:+17017666 MsG0680031265.01.T01:CDS
CCAAGTAATGATATGAGGTT+TGG 0.331279 6:-17014063 None:intergenic
ATAAAGGGTCAAGCAGGATA+TGG 0.382041 6:-17017591 None:intergenic
CTGTTCAACAAGTTTAGAAC+GGG 0.430395 6:-17013982 None:intergenic
GGCAAACCTTGGCAAGGAAG+AGG 0.430808 6:+17017687 MsG0680031265.01.T01:CDS
AGGTTTGCCTAGAAGCTCTT+AGG 0.436059 6:-17017673 None:intergenic
GATCTGAGACAAACACGGTT+TGG 0.440546 6:+17013892 MsG0680031265.01.T01:five_prime_UTR
ACATAAACACAACCATTTCA+CGG 0.443810 6:-17013844 None:intergenic
CAAGTAATGATATGAGGTTT+GGG 0.444710 6:-17014062 None:intergenic
TTCACTTAGCTTGTGCATAT+GGG 0.450193 6:-17017907 None:intergenic
TCTTTCCCTCTTCCTTGCCA+AGG 0.451193 6:-17017693 None:intergenic
CACCACAAAGAATGCTCATA+TGG 0.453210 6:-17017729 None:intergenic
CCAAACCTCATATCATTACT+TGG 0.454335 6:+17014063 MsG0680031265.01.T01:CDS
TGACAGCACTCAAATATTCA+TGG 0.456413 6:-17017817 None:intergenic
AATGTCACAGCAGAAGATCC+TGG 0.464356 6:-17018040 None:intergenic
ATTTATGCTGAGATCGGATC+TGG 0.468524 6:-17014016 None:intergenic
ATCACATCTCTATACTACTT+TGG 0.480081 6:-17018082 None:intergenic
TGCTGTGACATTTCTAGCAT+AGG 0.492979 6:+17018051 MsG0680031265.01.T01:three_prime_UTR
GAGTGTTACAAGTAAAATAA+GGG 0.495682 6:-17017939 None:intergenic
AATTACCAAGTAATGATATG+AGG 0.499125 6:-17014068 None:intergenic
GTCCATATGAGCATTCTTTG+TGG 0.501670 6:+17017727 MsG0680031265.01.T01:exon
GAGAATATTTATGCTGAGAT+CGG 0.512331 6:-17014022 None:intergenic
GAAATTTCATGCTTCTCTGT+AGG 0.523780 6:-17017973 None:intergenic
CCTAAAATAAAGGGTCAAGC+AGG 0.550751 6:-17017597 None:intergenic
AGTGCTGTCATTACTAGTGT+AGG 0.555939 6:+17017830 MsG0680031265.01.T01:three_prime_UTR
ATGTCACAGCAGAAGATCCT+GGG 0.557119 6:-17018039 None:intergenic
GCAAACCTTGGCAAGGAAGA+GGG 0.560246 6:+17017688 MsG0680031265.01.T01:CDS
TTGGGAGAGAAGAAAGTGAG+AGG 0.560855 6:-17014044 None:intergenic
TTAATGAATGAATGAAGAAG+AGG 0.576063 6:-17013781 None:intergenic
TTGCAGGGTTGATGTTGCTG+TGG 0.577557 6:+17017532 MsG0680031265.01.T01:intron
TCTTTGTGGTGATATCTCAC+AGG 0.583891 6:+17017741 MsG0680031265.01.T01:three_prime_UTR
AAGAGCTTCTAGGCAAACCT+TGG 0.584867 6:+17017676 MsG0680031265.01.T01:CDS
GCTGTTCAACAAGTTTAGAA+CGG 0.592461 6:-17013983 None:intergenic
CTTCTAGGCAAACCTTGGCA+AGG 0.624301 6:+17017681 MsG0680031265.01.T01:CDS
AAGAATGCTCATATGGACAA+TGG 0.637377 6:-17017722 None:intergenic
GGATGTACACTGGCAGACAA+AGG 0.645230 6:+17017762 MsG0680031265.01.T01:three_prime_UTR
ATATCTCACAGGATGTACAC+TGG 0.678712 6:+17017752 MsG0680031265.01.T01:three_prime_UTR
TATATGATCTGAGACAAACA+CGG 0.765222 6:+17013887 MsG0680031265.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCATACAAATATATAAA+AGG + Chr6:17016684-17016703 MsG0680031265.01.T01:intron 10.0%
!! ATATATATAATATGTCAAAA+TGG - Chr6:17015513-17015532 None:intergenic 10.0%
!! TATATATAATATGTCAAAAT+GGG - Chr6:17015512-17015531 None:intergenic 10.0%
!! TATATCTTACATAATTAATT+TGG + Chr6:17017790-17017809 MsG0680031265.01.T01:three_prime_UTR 10.0%
!!! ATGTATTTTTTTAAAAAACT+TGG + Chr6:17014364-17014383 MsG0680031265.01.T01:intron 10.0%
!!! CATATTATATATATGTTTTT+AGG + Chr6:17015519-17015538 MsG0680031265.01.T01:intron 10.0%
!!! CCAAATATTTTTTAAAAAAA+TGG - Chr6:17015988-17016007 None:intergenic 10.0%
!!! CCATTTTTTTAAAAAATATT+TGG + Chr6:17015985-17016004 MsG0680031265.01.T01:intron 10.0%
!! AAATTGAATTCCATATATTT+AGG - Chr6:17015748-17015767 None:intergenic 15.0%
!! AGTTTCTTCTATAATTTATT+TGG - Chr6:17017310-17017329 None:intergenic 15.0%
!! ATATATAATATGTCAAAATG+GGG - Chr6:17015511-17015530 None:intergenic 15.0%
!! ATATATTTGTATGAATTCAT+TGG - Chr6:17016681-17016700 None:intergenic 15.0%
!! TAAAAAAAAAAAAAAGTGAG+AGG - Chr6:17014100-17014119 None:intergenic 15.0%
!!! AAAAGTTTTGTTTGTTTAAA+AGG + Chr6:17014753-17014772 MsG0680031265.01.T01:intron 15.0%
!!! AAAGTTTTGTTTGTTTAAAA+GGG + Chr6:17014754-17014773 MsG0680031265.01.T01:intron 15.0%
!!! CTATTTTTAAAAGAAAAACA+GGG - Chr6:17014619-17014638 None:intergenic 15.0%
!!! TCATTGTATTTAATTTAACA+GGG - Chr6:17016157-17016176 None:intergenic 15.0%
!!! TGTATTTTGATATGATAAAA+AGG + Chr6:17016966-17016985 MsG0680031265.01.T01:intron 15.0%
!!! TTTAACCATATGAAATTTTA+AGG + Chr6:17015821-17015840 MsG0680031265.01.T01:intron 15.0%
!! AAGTTGTTAGTTAGTTAAAA+AGG + Chr6:17013938-17013957 MsG0680031265.01.T01:five_prime_UTR 20.0%
!! AGTTGTTAGTTAGTTAAAAA+GGG + Chr6:17013939-17013958 MsG0680031265.01.T01:five_prime_UTR 20.0%
!! TATCTAATAGGTAATAAATG+AGG + Chr6:17015671-17015690 MsG0680031265.01.T01:intron 20.0%
!! TTCCTTACTATAGAATATAT+AGG - Chr6:17017242-17017261 None:intergenic 20.0%
!!! AATTCCATATATTTAGGATT+GGG - Chr6:17015742-17015761 None:intergenic 20.0%
!!! CCTATTTTTAAAAGAAAAAC+AGG - Chr6:17014620-17014639 None:intergenic 20.0%
!!! CCTGTTTTTCTTTTAAAAAT+AGG + Chr6:17014617-17014636 MsG0680031265.01.T01:intron 20.0%
!!! GTCATTGTATTTAATTTAAC+AGG - Chr6:17016158-17016177 None:intergenic 20.0%
!!! TAGTATTGAAAATTTGAAAG+CGG - Chr6:17015905-17015924 None:intergenic 20.0%
!!! TATTGTAGATTTTTACAATG+AGG + Chr6:17018004-17018023 MsG0680031265.01.T01:three_prime_UTR 20.0%
!!! TGATTTTTGATCAAATACTA+TGG + Chr6:17017363-17017382 MsG0680031265.01.T01:intron 20.0%
!!! TTTTGCCTTAAAATTTCATA+TGG - Chr6:17015829-17015848 None:intergenic 20.0%
!!! TTTTTAAAAAACTTGGTATC+TGG + Chr6:17014371-17014390 MsG0680031265.01.T01:intron 20.0%
! AAAATACATTGAAGATGGTT+AGG + Chr6:17017467-17017486 MsG0680031265.01.T01:intron 25.0%
! AAAATGAAATATTAGCCCAA+AGG + Chr6:17015854-17015873 MsG0680031265.01.T01:intron 25.0%
! AATTACCAAGTAATGATATG+AGG - Chr6:17014071-17014090 None:intergenic 25.0%
! AATTTCCTTAATTTCTTGCA+GGG + Chr6:17017517-17017536 MsG0680031265.01.T01:intron 25.0%
! ACTATAAGTTAGCATATTTG+AGG - Chr6:17016188-17016207 None:intergenic 25.0%
! AGAAATTAAGGAAATTGTCA+AGG - Chr6:17017513-17017532 None:intergenic 25.0%
! ATGTAAATTCTTGACATTGA+GGG + Chr6:17015306-17015325 MsG0680031265.01.T01:intron 25.0%
! CAGATAAATCTTGATTTAGA+TGG + Chr6:17017195-17017214 MsG0680031265.01.T01:intron 25.0%
! CGTATAAAATACATTGAAGA+TGG + Chr6:17017462-17017481 MsG0680031265.01.T01:intron 25.0%
! CTTAAATCAAAAGGGTAAAT+GGG - Chr6:17015792-17015811 None:intergenic 25.0%
! GACCTATATATTCTATAGTA+AGG + Chr6:17017237-17017256 MsG0680031265.01.T01:intron 25.0%
! GAGTGTTACAAGTAAAATAA+GGG - Chr6:17017942-17017961 None:intergenic 25.0%
! GATGTAAAAATTTATGACTC+GGG + Chr6:17016478-17016497 MsG0680031265.01.T01:intron 25.0%
! GGATGTAAAAATTTATGACT+CGG + Chr6:17016477-17016496 MsG0680031265.01.T01:intron 25.0%
! GTTTGATTGTTCAAGAAAAA+TGG - Chr6:17016099-17016118 None:intergenic 25.0%
! TAAAGTACAAAACCATTGAT+TGG + Chr6:17016297-17016316 MsG0680031265.01.T01:intron 25.0%
! TACAAAATGTCCTCAAATTA+CGG + Chr6:17015040-17015059 MsG0680031265.01.T01:intron 25.0%
! TCACATGGTTTAAATTTCTT+AGG + Chr6:17016850-17016869 MsG0680031265.01.T01:intron 25.0%
! TTAATGAATGAATGAAGAAG+AGG - Chr6:17013784-17013803 None:intergenic 25.0%
! TTTGCTCTCTAGATTTAAAT+GGG - Chr6:17014423-17014442 None:intergenic 25.0%
!! ATAGGTTTTCGTTATCTAAT+AGG + Chr6:17015659-17015678 MsG0680031265.01.T01:intron 25.0%
!! ATTCCATATATTTAGGATTG+GGG - Chr6:17015741-17015760 None:intergenic 25.0%
!! ATTTAATTTAACAGGGAAGA+AGG - Chr6:17016150-17016169 None:intergenic 25.0%
!! ATTTTATAGGACAGATTGTT+GGG + Chr6:17017040-17017059 MsG0680031265.01.T01:intron 25.0%
!! GAATTCCATATATTTAGGAT+TGG - Chr6:17015743-17015762 None:intergenic 25.0%
!! TATTTTATAGGACAGATTGT+TGG + Chr6:17017039-17017058 MsG0680031265.01.T01:intron 25.0%
!! TTATCTGTCTTTTCTACAAT+AGG - Chr6:17017182-17017201 None:intergenic 25.0%
!! TTTAATTTAACAGGGAAGAA+GGG - Chr6:17016149-17016168 None:intergenic 25.0%
!!! AAGATTGATAGTTTTTCGAT+TGG + Chr6:17015185-17015204 MsG0680031265.01.T01:intron 25.0%
!!! AGATTGATAGTTTTTCGATT+GGG + Chr6:17015186-17015205 MsG0680031265.01.T01:intron 25.0%
!!! ATTCAGGTTGCATATAAATA+TGG + Chr6:17015569-17015588 MsG0680031265.01.T01:intron 25.0%
!!! GGTTTGTCTTTGAAATAAAA+TGG + Chr6:17015956-17015975 MsG0680031265.01.T01:intron 25.0%
AAGGAAATTGTCAAGGATAA+TGG - Chr6:17017506-17017525 None:intergenic 30.0%
AAGTGAGAAACATCATAATC+TGG - Chr6:17016577-17016596 None:intergenic 30.0%
AATTTAAACCATGTGACTTG+TGG - Chr6:17016846-17016865 None:intergenic 30.0%
ACAACGCAAAAGATATCTAA+TGG - Chr6:17016404-17016423 None:intergenic 30.0%
ACATAAACACAACCATTTCA+CGG - Chr6:17013847-17013866 None:intergenic 30.0%
ACTTCGTATATTGAAAGTGA+TGG + Chr6:17014570-17014589 MsG0680031265.01.T01:intron 30.0%
ACTTGTAACACTCGTAAAAT+TGG + Chr6:17017949-17017968 MsG0680031265.01.T01:three_prime_UTR 30.0%
AGTTTACATGAACAGATTCA+CGG - Chr6:17016227-17016246 None:intergenic 30.0%
ATAGTCACAAATTATGCCTA+TGG - Chr6:17014785-17014804 None:intergenic 30.0%
ATCACATCTCTATACTACTT+TGG - Chr6:17018085-17018104 None:intergenic 30.0%
ATTAGGTATGATTGCATTAG+AGG + Chr6:17017148-17017167 MsG0680031265.01.T01:intron 30.0%
CAAGTAATGATATGAGGTTT+GGG - Chr6:17014065-17014084 None:intergenic 30.0%
CAATTTCCTTAATTTCTTGC+AGG + Chr6:17017516-17017535 MsG0680031265.01.T01:intron 30.0%
CATGTAAATTCTTGACATTG+AGG + Chr6:17015305-17015324 MsG0680031265.01.T01:intron 30.0%
CCAAAAACGCCTAAAATAAA+GGG - Chr6:17017609-17017628 None:intergenic 30.0%
CGAGTGTTACAAGTAAAATA+AGG - Chr6:17017943-17017962 None:intergenic 30.0%
CTCAATGTCAAGAATTTACA+TGG - Chr6:17015307-17015326 None:intergenic 30.0%
CTTTGCTCTCTAGATTTAAA+TGG - Chr6:17014424-17014443 None:intergenic 30.0%
GAGAATATTTATGCTGAGAT+CGG - Chr6:17014025-17014044 None:intergenic 30.0%
GCTTAAATCAAAAGGGTAAA+TGG - Chr6:17015793-17015812 None:intergenic 30.0%
TAATAGGTAATAAATGAGGC+TGG + Chr6:17015675-17015694 MsG0680031265.01.T01:intron 30.0%
TAGTCACAAATTATGCCTAT+GGG - Chr6:17014784-17014803 None:intergenic 30.0%
TATTCATTCACTTTCAAACC+CGG + Chr6:17015364-17015383 MsG0680031265.01.T01:intron 30.0%
TCCAAAAACGCCTAAAATAA+AGG - Chr6:17017610-17017629 None:intergenic 30.0%
TCTGTCCTATAAAATACCAT+CGG - Chr6:17017035-17017054 None:intergenic 30.0%
TGATTGAACTCTTCATATTC+AGG + Chr6:17014842-17014861 MsG0680031265.01.T01:intron 30.0%
TGCAAAGGCTTAAATCAAAA+GGG - Chr6:17015800-17015819 None:intergenic 30.0%
TGTAAACCATCATATTCAAG+CGG + Chr6:17016908-17016927 MsG0680031265.01.T01:intron 30.0%
TTAAGGTTATGGAGTTATTC+AGG + Chr6:17015553-17015572 MsG0680031265.01.T01:intron 30.0%
TTAGGTATGATTGCATTAGA+GGG + Chr6:17017149-17017168 MsG0680031265.01.T01:intron 30.0%
TTCATATGGTTAAACTGCAA+AGG - Chr6:17015815-17015834 None:intergenic 30.0%
! ATTAGATATCTTTTGCGTTG+TGG + Chr6:17016403-17016422 MsG0680031265.01.T01:intron 30.0%
! TAAAGCCGATGGTATTTTAT+AGG + Chr6:17017027-17017046 MsG0680031265.01.T01:intron 30.0%
! TAGCATTGAGATAAAATGAC+AGG + Chr6:17015332-17015351 MsG0680031265.01.T01:intron 30.0%
! TATATGATCTGAGACAAACA+CGG + Chr6:17013887-17013906 MsG0680031265.01.T01:five_prime_UTR 30.0%
! TCTAAAACAACTACTTGTGT+CGG - Chr6:17014253-17014272 None:intergenic 30.0%
! TTCCATATATTTAGGATTGG+GGG - Chr6:17015740-17015759 None:intergenic 30.0%
! TTGCTGTTAAAATGCAAAAC+TGG + Chr6:17016737-17016756 MsG0680031265.01.T01:intron 30.0%
! TTTTCTTGAACAATCAAACC+CGG + Chr6:17016100-17016119 MsG0680031265.01.T01:intron 30.0%
!! AAAAGGTACTCTCAAACTAA+AGG + Chr6:17016983-17017002 MsG0680031265.01.T01:intron 30.0%
!! AAACCATTTTCCTTAGCTTT+TGG - Chr6:17015941-17015960 None:intergenic 30.0%
!! AACCATTTTCCTTAGCTTTT+GGG - Chr6:17015940-17015959 None:intergenic 30.0%
!! ACTGTATTTTTACGATTTGG+CGG - Chr6:17013808-17013827 None:intergenic 30.0%
!! ATAGTTTTTCGATTGGGTTA+TGG + Chr6:17015192-17015211 MsG0680031265.01.T01:intron 30.0%
!! GAGACTGTATTTTTACGATT+TGG - Chr6:17013811-17013830 None:intergenic 30.0%
!! TAGTTTTTCGATTGGGTTAT+GGG + Chr6:17015193-17015212 MsG0680031265.01.T01:intron 30.0%
!! TGTAAAGTTGATATGCAATC+AGG + Chr6:17016432-17016451 MsG0680031265.01.T01:intron 30.0%
!!! CACTAGTTTTATTCATACGT+TGG - Chr6:17017075-17017094 None:intergenic 30.0%
AAAACTGGATTTCTGATTCC+AGG + Chr6:17016752-17016771 MsG0680031265.01.T01:intron 35.0%
AAATGATGGTAGAATGAGTG+AGG + Chr6:17016884-17016903 MsG0680031265.01.T01:intron 35.0%
AAATTAAGACTTGAGTTCCC+CGG - Chr6:17016122-17016141 None:intergenic 35.0%
AAGAATGCTCATATGGACAA+TGG - Chr6:17017725-17017744 None:intergenic 35.0%
AAGTAGTCACTTCAGATCAA+AGG - Chr6:17014339-17014358 None:intergenic 35.0%
AATGTGTAGAAATACTGGAC+GGG + Chr6:17017120-17017139 MsG0680031265.01.T01:intron 35.0%
AGAATTTACATGGACAAGGA+AGG - Chr6:17015297-17015316 None:intergenic 35.0%
AGATATAGTGATAGCATCAG+TGG - Chr6:17016262-17016281 None:intergenic 35.0%
AGTCACAAATTATGCCTATG+GGG - Chr6:17014783-17014802 None:intergenic 35.0%
ATCAACCCTGCAAGAAATTA+AGG - Chr6:17017525-17017544 None:intergenic 35.0%
ATTCATTCACTTTCAAACCC+GGG + Chr6:17015365-17015384 MsG0680031265.01.T01:intron 35.0%
ATTGAAACCGAGACTTTGTT+TGG + Chr6:17014542-17014561 MsG0680031265.01.T01:intron 35.0%
CAATGCACTTTGCCTTTATT+GGG + Chr6:17016062-17016081 MsG0680031265.01.T01:intron 35.0%
CACTGAATGTGTAGAAATAC+TGG + Chr6:17017115-17017134 MsG0680031265.01.T01:intron 35.0%
CACTGCAATTAACTAGTGTT+GGG + Chr6:17014896-17014915 MsG0680031265.01.T01:intron 35.0%
CCAAACCTCATATCATTACT+TGG + Chr6:17014063-17014082 MsG0680031265.01.T01:CDS 35.0%
CCAAGTAATGATATGAGGTT+TGG - Chr6:17014066-17014085 None:intergenic 35.0%
CTGCAAAGGCTTAAATCAAA+AGG - Chr6:17015801-17015820 None:intergenic 35.0%
GAAATTTCATGCTTCTCTGT+AGG - Chr6:17017976-17017995 None:intergenic 35.0%
GAATGTATTCACATGTGTGA+GGG + Chr6:17014287-17014306 MsG0680031265.01.T01:intron 35.0%
GAATGTGTAGAAATACTGGA+CGG + Chr6:17017119-17017138 MsG0680031265.01.T01:intron 35.0%
GATGTAGCACTATATTGTAG+CGG + Chr6:17014976-17014995 MsG0680031265.01.T01:intron 35.0%
GATTTGCAAAAACCGTGAAA+TGG + Chr6:17013832-17013851 MsG0680031265.01.T01:five_prime_UTR 35.0%
GCAGAAAACTCAACATAGTA+TGG - Chr6:17015401-17015420 None:intergenic 35.0%
GCTGTTCAACAAGTTTAGAA+CGG - Chr6:17013986-17014005 None:intergenic 35.0%
GGAGCTATAAAATTGGCTAA+GGG + Chr6:17016347-17016366 MsG0680031265.01.T01:intron 35.0%
GTAAACCATCATATTCAAGC+GGG + Chr6:17016909-17016928 MsG0680031265.01.T01:intron 35.0%
GTCAAGAATTTACATGGACA+AGG - Chr6:17015301-17015320 None:intergenic 35.0%
GTGGTTGAAAATTGAAATGG+AGG + Chr6:17016932-17016951 MsG0680031265.01.T01:intron 35.0%
GTTGAAAATTGAAATGGAGG+AGG + Chr6:17016935-17016954 MsG0680031265.01.T01:intron 35.0%
TAAACCATCATATTCAAGCG+GGG + Chr6:17016910-17016929 MsG0680031265.01.T01:intron 35.0%
TATGCCCATTTCAATGTTTC+AGG - Chr6:17014486-17014505 None:intergenic 35.0%
TCAATGCACTTTGCCTTTAT+TGG + Chr6:17016061-17016080 MsG0680031265.01.T01:intron 35.0%
TCTACCATCATTTCGTACAA+TGG - Chr6:17016877-17016896 None:intergenic 35.0%
TGAATGTATTCACATGTGTG+AGG + Chr6:17014286-17014305 MsG0680031265.01.T01:intron 35.0%
TGACAGCACTCAAATATTCA+TGG - Chr6:17017820-17017839 None:intergenic 35.0%
TGCAATAAGATCTTGCACAA+GGG - Chr6:17016654-17016673 None:intergenic 35.0%
TGGAGCTATAAAATTGGCTA+AGG + Chr6:17016346-17016365 MsG0680031265.01.T01:intron 35.0%
TTTCCAGTAGCAAAACCTTT+GGG - Chr6:17015872-17015891 None:intergenic 35.0%
TTTCTTGAACAATCAAACCC+GGG + Chr6:17016101-17016120 MsG0680031265.01.T01:intron 35.0%
! ACATCTGTTGTGAGCTTTAT+AGG + Chr6:17015641-17015660 MsG0680031265.01.T01:intron 35.0%
! AGGGTGTTCTGTATTCTATT+GGG + Chr6:17016456-17016475 MsG0680031265.01.T01:intron 35.0%
! AGTTGATATGCAATCAGGTA+GGG + Chr6:17016437-17016456 MsG0680031265.01.T01:intron 35.0%
! CAAACAAAACTTTTCCCACT+AGG - Chr6:17014747-17014766 None:intergenic 35.0%
! CAGTACTGGAGCTATAAAAT+TGG + Chr6:17016340-17016359 MsG0680031265.01.T01:intron 35.0%
! CTGTTCAACAAGTTTAGAAC+GGG - Chr6:17013985-17014004 None:intergenic 35.0%
! CTTAAAGTGTTTCACCTAGT+GGG + Chr6:17014730-17014749 MsG0680031265.01.T01:intron 35.0%
! TACTATGTTGAGTTTTCTGC+TGG + Chr6:17015401-17015420 MsG0680031265.01.T01:intron 35.0%
! TAGGGTGTTCTGTATTCTAT+TGG + Chr6:17016455-17016474 MsG0680031265.01.T01:intron 35.0%
! TCTTAAAGTGTTTCACCTAG+TGG + Chr6:17014729-17014748 MsG0680031265.01.T01:intron 35.0%
! TTGAAACCGAGACTTTGTTT+GGG + Chr6:17014543-17014562 MsG0680031265.01.T01:intron 35.0%
! TTTTCCTGAGATTTAACAGC+AGG - Chr6:17015598-17015617 None:intergenic 35.0%
!! AAGTTGATATGCAATCAGGT+AGG + Chr6:17016436-17016455 MsG0680031265.01.T01:intron 35.0%
!! ACCATTTTCCTTAGCTTTTG+GGG - Chr6:17015939-17015958 None:intergenic 35.0%
!! ATTCACTTAGCTTGTGCATA+TGG - Chr6:17017911-17017930 None:intergenic 35.0%
!! TTCACTTAGCTTGTGCATAT+GGG - Chr6:17017910-17017929 None:intergenic 35.0%
!!! CCCTTTATTTTAGGCGTTTT+TGG + Chr6:17017606-17017625 MsG0680031265.01.T01:CDS 35.0%
!!! GCTGCATTTTTAGGTTAGAA+AGG + Chr6:17015705-17015724 MsG0680031265.01.T01:intron 35.0%
AAAGGGTAAATGGGAAAGTC+TGG - Chr6:17015783-17015802 None:intergenic 40.0%
AAAGTAGATCAGACGAAGGA+TGG + Chr6:17017260-17017279 MsG0680031265.01.T01:intron 40.0%
AAGCTTCGATAGCTTAGACA+TGG + Chr6:17016800-17016819 MsG0680031265.01.T01:intron 40.0%
AAGGAAAGTAGATCAGACGA+AGG + Chr6:17017256-17017275 MsG0680031265.01.T01:intron 40.0%
AATTAAGACTTGAGTTCCCC+GGG - Chr6:17016121-17016140 None:intergenic 40.0%
ACATCTCTCATTCTTTCAGC+TGG - Chr6:17014710-17014729 None:intergenic 40.0%
ACCACTAAAGCCGTAATTTG+AGG - Chr6:17015053-17015072 None:intergenic 40.0%
AGGTCCATTGTACGAAATGA+TGG + Chr6:17016870-17016889 MsG0680031265.01.T01:intron 40.0%
AGTGCTGTCATTACTAGTGT+AGG + Chr6:17017830-17017849 MsG0680031265.01.T01:three_prime_UTR 40.0%
ATAAAGGGTCAAGCAGGATA+TGG - Chr6:17017594-17017613 None:intergenic 40.0%
ATCCTCGAAATAGTGTAGGT+AGG - Chr6:17015453-17015472 None:intergenic 40.0%
ATGTCATACCACAAGTCACA+TGG + Chr6:17016835-17016854 MsG0680031265.01.T01:intron 40.0%
ATTCCAGGCAGGATTTATGT+AGG + Chr6:17016767-17016786 MsG0680031265.01.T01:intron 40.0%
ATTGAAATGGGCATACTCCA+AGG + Chr6:17014491-17014510 MsG0680031265.01.T01:intron 40.0%
ATTTATGCTGAGATCGGATC+TGG - Chr6:17014019-17014038 None:intergenic 40.0%
CACCACAAAGAATGCTCATA+TGG - Chr6:17017732-17017751 None:intergenic 40.0%
CAGGATTTATGTAGGTCATG+TGG + Chr6:17016775-17016794 MsG0680031265.01.T01:intron 40.0%
CATGTAGACCTAGTGGAATA+AGG + Chr6:17017397-17017416 MsG0680031265.01.T01:intron 40.0%
CCAACACTAGTTAATTGCAG+TGG - Chr6:17014898-17014917 None:intergenic 40.0%
CCACTGCAATTAACTAGTGT+TGG + Chr6:17014895-17014914 MsG0680031265.01.T01:intron 40.0%
CCATAACCTTAAAGCACAAG+AGG - Chr6:17015545-17015564 None:intergenic 40.0%
CCATCAACACTCAAAAACGT+AGG - Chr6:17017872-17017891 None:intergenic 40.0%
CCTAAAATAAAGGGTCAAGC+AGG - Chr6:17017600-17017619 None:intergenic 40.0%
CGGACCTGAAACATTGAAAT+GGG + Chr6:17014479-17014498 MsG0680031265.01.T01:intron 40.0%
CTACATCCTCGAAATAGTGT+AGG - Chr6:17015457-17015476 None:intergenic 40.0%
CTTATTCCACTAGGTCTACA+TGG - Chr6:17017399-17017418 None:intergenic 40.0%
CTTTCCAGTAGCAAAACCTT+TGG - Chr6:17015873-17015892 None:intergenic 40.0%
CTTTGCCTTTATTGGGATCA+CGG + Chr6:17016069-17016088 MsG0680031265.01.T01:intron 40.0%
GATAGCTTAGACATGGATGT+TGG + Chr6:17016807-17016826 MsG0680031265.01.T01:intron 40.0%
GGATGTAGTCGAAATTATGC+TGG + Chr6:17015469-17015488 MsG0680031265.01.T01:intron 40.0%
GGGGTGGTTGAAAATTGAAA+TGG + Chr6:17016929-17016948 MsG0680031265.01.T01:intron 40.0%
GTAAGTCCCAAACAAAGTCT+CGG - Chr6:17014552-17014571 None:intergenic 40.0%
GTCCATATGAGCATTCTTTG+TGG + Chr6:17017727-17017746 MsG0680031265.01.T01:exon 40.0%
GTGCAATAAGATCTTGCACA+AGG - Chr6:17016655-17016674 None:intergenic 40.0%
TAAGATTCATCCTTAGCAGG+TGG - Chr6:17014687-17014706 None:intergenic 40.0%
TAATGAAATGCCACCTGCTA+AGG + Chr6:17014674-17014693 MsG0680031265.01.T01:intron 40.0%
TCAATGACTGACTAATCCGA+GGG + Chr6:17014395-17014414 MsG0680031265.01.T01:intron 40.0%
TCGGACCTGAAACATTGAAA+TGG + Chr6:17014478-17014497 MsG0680031265.01.T01:intron 40.0%
TCTTTGTGGTGATATCTCAC+AGG + Chr6:17017741-17017760 MsG0680031265.01.T01:three_prime_UTR 40.0%
TGAACCTTGGTCAACTAACA+TGG - Chr6:17016612-17016631 None:intergenic 40.0%
TGCCCCCAATCCTAAATATA+TGG + Chr6:17015735-17015754 MsG0680031265.01.T01:intron 40.0%
TGGAAGTAGATCGTGATCTT+AGG - Chr6:17014878-17014897 None:intergenic 40.0%
TGGTAAGATTCATCCTTAGC+AGG - Chr6:17014690-17014709 None:intergenic 40.0%
TGTTCCATGTTAGTTGACCA+AGG + Chr6:17016605-17016624 MsG0680031265.01.T01:intron 40.0%
TTAAGCTGTGGTCCAATCAA+TGG - Chr6:17016312-17016331 None:intergenic 40.0%
TTATAGGACAGATTGTTGGG+TGG + Chr6:17017043-17017062 MsG0680031265.01.T01:intron 40.0%
TTCAATGACTGACTAATCCG+AGG + Chr6:17014394-17014413 MsG0680031265.01.T01:intron 40.0%
TTCTTGAACAATCAAACCCG+GGG + Chr6:17016102-17016121 MsG0680031265.01.T01:intron 40.0%
TTTAGGCCTCTTGTGCTTTA+AGG + Chr6:17015536-17015555 MsG0680031265.01.T01:intron 40.0%
! AAATGGGAAAGTCTGGAACT+AGG - Chr6:17015776-17015795 None:intergenic 40.0%
! ATGGGTTCTGTTGTTGTTGT+GGG + Chr6:17017421-17017440 MsG0680031265.01.T01:intron 40.0%
! ATGTGACTTGTGGTATGACA+TGG - Chr6:17016836-17016855 None:intergenic 40.0%
! GTAGGTAGGTGTTATGCTTA+TGG - Chr6:17015439-17015458 None:intergenic 40.0%
! TCCTCAAATTACGGCTTTAG+TGG + Chr6:17015049-17015068 MsG0680031265.01.T01:intron 40.0%
! TGAGGAATTTCCTTTTTCCC+AGG + Chr6:17018022-17018041 MsG0680031265.01.T01:three_prime_UTR 40.0%
! TTAGATGGTTTGTGCGTATG+TGG + Chr6:17017210-17017229 MsG0680031265.01.T01:intron 40.0%
! TTTTATAGCTCCAGTACTGC+CGG - Chr6:17016339-17016358 None:intergenic 40.0%
!! CCATTTTCCTTAGCTTTTGG+GGG - Chr6:17015938-17015957 None:intergenic 40.0%
!! CCTCTTGTGCTTTAAGGTTA+TGG + Chr6:17015542-17015561 MsG0680031265.01.T01:intron 40.0%
!! GTTTAAAAGGGTATCCCCAT+AGG + Chr6:17014766-17014785 MsG0680031265.01.T01:intron 40.0%
!! TGCTGTGACATTTCTAGCAT+AGG + Chr6:17018051-17018070 MsG0680031265.01.T01:three_prime_UTR 40.0%
!!! CCTACGTTTTTGAGTGTTGA+TGG + Chr6:17017869-17017888 MsG0680031265.01.T01:three_prime_UTR 40.0%
!!! CCTGCTTGACCCTTTATTTT+AGG + Chr6:17017597-17017616 MsG0680031265.01.T01:CDS 40.0%
AAGAGCTTCTAGGCAAACCT+TGG + Chr6:17017676-17017695 MsG0680031265.01.T01:CDS 45.0%
AATGACTGACTAATCCGAGG+GGG + Chr6:17014397-17014416 MsG0680031265.01.T01:intron 45.0%
AATGTCACAGCAGAAGATCC+TGG - Chr6:17018043-17018062 None:intergenic 45.0%
AGACCTAGTGGAATAAGGCA+TGG + Chr6:17017402-17017421 MsG0680031265.01.T01:intron 45.0%
AGCAGAAGATCCTGGGAAAA+AGG - Chr6:17018035-17018054 None:intergenic 45.0%
AGGAGTCCACCATCAAATTC+TGG - Chr6:17015277-17015296 None:intergenic 45.0%
AGGTTTGCCTAGAAGCTCTT+AGG - Chr6:17017676-17017695 None:intergenic 45.0%
AGTCCACCATCAAATTCTGG+GGG - Chr6:17015274-17015293 None:intergenic 45.0%
ATACTGGACGGGATGAGATT+AGG + Chr6:17017131-17017150 MsG0680031265.01.T01:intron 45.0%
ATCGAAACCCCCAAAAGCTA+AGG + Chr6:17015928-17015947 MsG0680031265.01.T01:intron 45.0%
ATGTCACAGCAGAAGATCCT+GGG - Chr6:17018042-17018061 None:intergenic 45.0%
ATTGAAATGGAGGAGGCTCT+TGG + Chr6:17016942-17016961 MsG0680031265.01.T01:intron 45.0%
CAATGACTGACTAATCCGAG+GGG + Chr6:17014396-17014415 MsG0680031265.01.T01:intron 45.0%
CACCTACCTACACTATTTCG+AGG + Chr6:17015448-17015467 MsG0680031265.01.T01:intron 45.0%
CCCCCAAAAGCTAAGGAAAA+TGG + Chr6:17015935-17015954 MsG0680031265.01.T01:intron 45.0%
CCTTACAACACTAAAGCCGA+TGG + Chr6:17017016-17017035 MsG0680031265.01.T01:intron 45.0%
CTAGAGAGCAAAGCTCTGTA+AGG + Chr6:17014431-17014450 MsG0680031265.01.T01:intron 45.0%
GACACCTGCTGTTAAATCTC+AGG + Chr6:17015591-17015610 MsG0680031265.01.T01:intron 45.0%
GACCTAGTGGAATAAGGCAT+GGG + Chr6:17017403-17017422 MsG0680031265.01.T01:intron 45.0%
GAGATCCGTGATCCCAATAA+AGG - Chr6:17016077-17016096 None:intergenic 45.0%
GAGTCCACCATCAAATTCTG+GGG - Chr6:17015275-17015294 None:intergenic 45.0%
GCAAGACTAACCTTGAGACT+GGG + Chr6:17014805-17014824 MsG0680031265.01.T01:intron 45.0%
GGAGTCCACCATCAAATTCT+GGG - Chr6:17015276-17015295 None:intergenic 45.0%
GTTGATCCATGTAGACCTAG+TGG + Chr6:17017390-17017409 MsG0680031265.01.T01:intron 45.0%
GTTTCAATTCAACTCCGTCG+TGG - Chr6:17014530-17014549 None:intergenic 45.0%
TAGATTTAAATGGGCCCCCT+CGG - Chr6:17014414-17014433 None:intergenic 45.0%
TAGTATGGCATTCAGATGCC+CGG - Chr6:17015386-17015405 None:intergenic 45.0%
TCATACGCCTAAGAGCTTCT+AGG + Chr6:17017666-17017685 MsG0680031265.01.T01:CDS 45.0%
TGACCTACATAAATCCTGCC+TGG - Chr6:17016773-17016792 None:intergenic 45.0%
TGCAAGACTAACCTTGAGAC+TGG + Chr6:17014804-17014823 MsG0680031265.01.T01:intron 45.0%
TTGAAATGGAGGAGGCTCTT+GGG + Chr6:17016943-17016962 MsG0680031265.01.T01:intron 45.0%
TTGACCAAGGTTCATAACCC+TGG + Chr6:17016618-17016637 MsG0680031265.01.T01:intron 45.0%
TTGGGAGAGAAGAAAGTGAG+AGG - Chr6:17014047-17014066 None:intergenic 45.0%
TTTATAGCTCCAGTACTGCC+GGG - Chr6:17016338-17016357 None:intergenic 45.0%
! AACCCATGCCTTATTCCACT+AGG - Chr6:17017408-17017427 None:intergenic 45.0%
! ATTCACATGTGTGAGGGTGT+TGG + Chr6:17014293-17014312 MsG0680031265.01.T01:intron 45.0%
! CAAAGGCGTGTCAAACTATC+TGG - Chr6:17014322-17014341 None:intergenic 45.0%
! CATGGGTTCTGTTGTTGTTG+TGG + Chr6:17017420-17017439 MsG0680031265.01.T01:intron 45.0%
! GATCTGAGACAAACACGGTT+TGG + Chr6:17013892-17013911 MsG0680031265.01.T01:five_prime_UTR 45.0%
! GCTTTATCGCTAGAAGCACA+AGG + Chr6:17017282-17017301 MsG0680031265.01.T01:intron 45.0%
! GGTGTGACAGCTGCATTTTT+AGG + Chr6:17015696-17015715 MsG0680031265.01.T01:intron 45.0%
! TAGCCCAAAGGTTTTGCTAC+TGG + Chr6:17015866-17015885 MsG0680031265.01.T01:intron 45.0%
!! CCATCGGCTTTAGTGTTGTA+AGG - Chr6:17017019-17017038 None:intergenic 45.0%
!! CTCTGTAAGGACTAGCCTAT+TGG + Chr6:17014444-17014463 MsG0680031265.01.T01:intron 45.0%
!!! ATTTTTTTAAAAAATATTTG+GGG + Chr6:17015987-17016006 MsG0680031265.01.T01:intron 5.0%
!!! CATTTTTTTAAAAAATATTT+GGG + Chr6:17015986-17016005 MsG0680031265.01.T01:intron 5.0%
ACCACCCCGCTTGAATATGA+TGG - Chr6:17016917-17016936 None:intergenic 50.0%
ACCATCATATTCAAGCGGGG+TGG + Chr6:17016913-17016932 MsG0680031265.01.T01:intron 50.0%
AGTATGGCATTCAGATGCCC+GGG - Chr6:17015385-17015404 None:intergenic 50.0%
ATTCAACTCCGTCGTGGTGT+TGG - Chr6:17014524-17014543 None:intergenic 50.0%
CGATACCCCCCAGAATTTGA+TGG + Chr6:17015265-17015284 MsG0680031265.01.T01:intron 50.0%
CTAACCACTACCCAGTCTCA+AGG - Chr6:17014818-17014837 None:intergenic 50.0%
CTAACCTTGAGACTGGGTAG+TGG + Chr6:17014811-17014830 MsG0680031265.01.T01:intron 50.0%
CTGGATTTCTGATTCCAGGC+AGG + Chr6:17016756-17016775 MsG0680031265.01.T01:intron 50.0%
CTTCTAGGCAAACCTTGGCA+AGG + Chr6:17017681-17017700 MsG0680031265.01.T01:CDS 50.0%
GCAAACCTTGGCAAGGAAGA+GGG + Chr6:17017688-17017707 MsG0680031265.01.T01:CDS 50.0%
GTCCACCATCAAATTCTGGG+GGG - Chr6:17015273-17015292 None:intergenic 50.0%
TACCCCCCAGAATTTGATGG+TGG + Chr6:17015268-17015287 MsG0680031265.01.T01:intron 50.0%
TCTTTCCCTCTTCCTTGCCA+AGG - Chr6:17017696-17017715 None:intergenic 50.0%
! CCTATTGGCACTGGAGGAAT+CGG + Chr6:17014459-17014478 MsG0680031265.01.T01:intron 50.0%
! CTTGAGACTGGGTAGTGGTT+AGG + Chr6:17014816-17014835 MsG0680031265.01.T01:intron 50.0%
! GATGGTTTGTGCGTATGTGG+AGG + Chr6:17017213-17017232 MsG0680031265.01.T01:intron 50.0%
! GGGAAAGTCTGGAACTAGGA+CGG - Chr6:17015772-17015791 None:intergenic 50.0%
!! AAGGACTAGCCTATTGGCAC+TGG + Chr6:17014450-17014469 MsG0680031265.01.T01:intron 50.0%
!! ATGAAGCACTGACACAGACG+CGG + Chr6:17014224-17014243 MsG0680031265.01.T01:intron 50.0%
!! GGGGGTATCGAAAACTAGCT+GGG - Chr6:17015255-17015274 None:intergenic 50.0%
!! TTAGTGGTGCCATAGCACTG+CGG + Chr6:17015065-17015084 MsG0680031265.01.T01:intron 50.0%
!! TTGCAGGGTTGATGTTGCTG+TGG + Chr6:17017532-17017551 MsG0680031265.01.T01:intron 50.0%
CCAAGGTTCATAACCCTGGC+AGG + Chr6:17016622-17016641 MsG0680031265.01.T01:intron 55.0%
CCGATTCCTCCAGTGCCAAT+AGG - Chr6:17014462-17014481 None:intergenic 55.0%
CCTGCCAGGGTTATGAACCT+TGG - Chr6:17016625-17016644 None:intergenic 55.0%
GGCAAACCTTGGCAAGGAAG+AGG + Chr6:17017687-17017706 MsG0680031265.01.T01:CDS 55.0%
GTGCTACATCGCTGCTGTTG+TGG - Chr6:17014966-17014985 None:intergenic 55.0%
TCTGCTACACCGCAGTGCTA+TGG - Chr6:17015077-17015096 None:intergenic 55.0%
TGGACCACAGCTTAAGAGCC+CGG + Chr6:17016317-17016336 MsG0680031265.01.T01:intron 55.0%
!! GACTAGCCTATTGGCACTGG+AGG + Chr6:17014453-17014472 MsG0680031265.01.T01:intron 55.0%
!! GGGGGGTATCGAAAACTAGC+TGG - Chr6:17015256-17015275 None:intergenic 55.0%
ACTGCCGGGCTCTTAAGCTG+TGG - Chr6:17016324-17016343 None:intergenic 60.0%
GCTTAAGAGCCCGGCAGTAC+TGG + Chr6:17016326-17016345 MsG0680031265.01.T01:intron 60.0%
GGTTCATAACCCTGGCAGGC+TGG + Chr6:17016626-17016645 MsG0680031265.01.T01:intron 60.0%
GTCCCAAGCCAACACCACGA+CGG + Chr6:17014513-17014532 MsG0680031265.01.T01:intron 60.0%
!! ACTCCGTCGTGGTGTTGGCT+TGG - Chr6:17014519-17014538 None:intergenic 60.0%
!! CTCCGTCGTGGTGTTGGCTT+GGG - Chr6:17014518-17014537 None:intergenic 60.0%
!! GTGGTGTTGGCTTGGGACCT+TGG - Chr6:17014511-17014530 None:intergenic 60.0%
ACAAGGGAGCCAGCCTGCCA+GGG - Chr6:17016638-17016657 None:intergenic 65.0%
CACAAGGGAGCCAGCCTGCC+AGG - Chr6:17016639-17016658 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 17013769 17018155 17013769 ID=MsG0680031265.01;Name=MsG0680031265.01
Chr6 mRNA 17013769 17018155 17013769 ID=MsG0680031265.01.T01;Parent=MsG0680031265.01;Name=MsG0680031265.01.T01;_AED=0.40;_eAED=0.40;_QI=206|1|1|1|0|0|2|419|102
Chr6 exon 17013769 17014084 17013769 ID=MsG0680031265.01.T01:exon:27752;Parent=MsG0680031265.01.T01
Chr6 exon 17017538 17018155 17017538 ID=MsG0680031265.01.T01:exon:27753;Parent=MsG0680031265.01.T01
Chr6 five_prime_UTR 17013769 17013974 17013769 ID=MsG0680031265.01.T01:five_prime_utr;Parent=MsG0680031265.01.T01
Chr6 CDS 17013975 17014084 17013975 ID=MsG0680031265.01.T01:cds;Parent=MsG0680031265.01.T01
Chr6 CDS 17017538 17017736 17017538 ID=MsG0680031265.01.T01:cds;Parent=MsG0680031265.01.T01
Chr6 three_prime_UTR 17017737 17018155 17017737 ID=MsG0680031265.01.T01:three_prime_utr;Parent=MsG0680031265.01.T01
Gene Sequence

>MsG0680031265.01.T01

ATGATAACCCGTTCTAAACTTGTTGAACAGCTCAGAGATTACCAGATCCGATCTCAGCATAAATATTCTCCTCTCACTTTCTTCTCTCCCAAACCTCATATCATTACTTGGGTTGATGTTGCTGTGGCAATTGCTTTTGCTCTAGTGTTCTGCATGCTTGTCGCCATATCCTGCTTGACCCTTTATTTTAGGCGTTTTTGGATTTTTCTTCTTGTTGTAAGCTTCAGTATAATTCTTCTCATACGCCTAAGAGCTTCTAGGCAAACCTTGGCAAGGAAGAGGGAAAGAAGATTGCCATTGTCCATATGA
Protein sequence

>MsG0680031265.01.T01

MITRSKLVEQLRDYQIRSQHKYSPLTFFSPKPHIITWVDVAVAIAFALVFCMLVAISCLTLYFRRFWIFLLVVSFSIILLIRLRASRQTLARKRERRLPLSI*