AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031330.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031330.01.T01 MTR_6g029230 99.432 176 1 0 6 181 51 226 3.11e-133 372
MsG0680031330.01.T01 MTR_2g018260 35.616 146 94 0 6 151 53 198 7.76e-33 117
MsG0680031330.01.T01 MTR_1g045130 33.333 144 95 1 1 143 29 172 9.18e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031330.01.T01 AT5G18110 86.782 174 23 0 8 181 48 221 3.45e-116 328
MsG0680031330.01.T01 AT4G18040 37.063 143 90 0 9 151 66 208 2.58e-31 113
MsG0680031330.01.T01 AT1G29550 34.247 146 96 0 6 151 68 213 7.13e-27 102
MsG0680031330.01.T01 AT1G29590 32.877 146 98 0 6 151 68 213 1.83e-26 101
MsG0680031330.01.T01 AT5G35620 30.539 167 107 4 8 169 31 193 5.02e-23 91.3
MsG0680031330.01.T01 AT5G35620 30.539 167 107 4 8 169 31 193 5.02e-23 91.3
MsG0680031330.01.T01 AT5G35620 31.655 139 88 3 8 141 31 167 7.71e-22 87.4

Find 60 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAATAGGTTGAAGGATTT+TGG 0.117111 6:-18320966 MsG0680031330.01.T01:intron
AAGAAGGAATTCGTCCTTTA+TGG 0.158078 6:-18320879 MsG0680031330.01.T01:CDS
AACCTAATGTTGGGTGTTTC+AGG 0.228687 6:-18318425 MsG0680031330.01.T01:three_prime_UTR
ATCAGAGCAAATTTGTGTTT+TGG 0.293172 6:-18321141 MsG0680031330.01.T01:intron
CTAACTGCAATGGTGGAAAA+TGG 0.299336 6:-18320705 MsG0680031330.01.T01:CDS
TGCGAGACAATTCATCTTAC+AGG 0.303636 6:-18318555 MsG0680031330.01.T01:CDS
ATAAAGGACGAATTCCTTCT+TGG 0.312769 6:+18320881 None:intergenic
GTGGGTGACCAATTGGATTA+TGG 0.334671 6:-18318837 MsG0680031330.01.T01:CDS
TATGATTGGTTTCGAATTCC+AGG 0.339307 6:+18321103 None:intergenic
ATGGATTATACGATTCAAAA+AGG 0.339986 6:-18320686 MsG0680031330.01.T01:CDS
TGCCTTGGTGGGTGACCAAT+TGG 0.355029 6:-18318844 MsG0680031330.01.T01:CDS
TACCTTCCGAGACTGCTTCA+TGG 0.385734 6:+18321944 None:intergenic
GCTTCCTCACGGCTATGTTA+TGG 0.396161 6:-18318602 MsG0680031330.01.T01:CDS
TAATGTTATCCTCGTATGAT+TGG 0.409837 6:+18321089 None:intergenic
AGAAGGAATTCGTCCTTTAT+GGG 0.411121 6:-18320878 MsG0680031330.01.T01:CDS
AGATGCAAATCTGTTGGAAT+AGG 0.415770 6:+18320907 None:intergenic
AATGCAGGTGCTTGCCTTGG+TGG 0.418746 6:-18318856 MsG0680031330.01.T01:intron
CATAGCCGTGAGGAAGCTTT+AGG 0.427935 6:+18318608 None:intergenic
TCGCAATGCCTCTGATCATC+AGG 0.442213 6:-18318751 MsG0680031330.01.T01:intron
TGTTATCACCATAATCCAAT+TGG 0.452588 6:+18318829 None:intergenic
TGGAAAAGATGCAAATCTGT+TGG 0.462641 6:+18320901 None:intergenic
TAAGTATCTACCTCCCATAA+AGG 0.467917 6:+18320865 None:intergenic
ACGAGGACATAGTCAGTGTC+TGG 0.468461 6:-18318776 MsG0680031330.01.T01:CDS
GTCCTGAAACACCCAACATT+AGG 0.474595 6:+18318423 None:intergenic
TGGTACACTCGTCGGGTACC+TGG 0.476680 6:-18321121 MsG0680031330.01.T01:CDS
AATGTTGGGTGTTTCAGGAC+TGG 0.483387 6:-18318420 MsG0680031330.01.T01:three_prime_UTR
TAGCATCAACCGAGGGGACT+AGG 0.490917 6:+18318493 None:intergenic
GATTATGGTGATAACATTTG+TGG 0.503544 6:-18318822 MsG0680031330.01.T01:CDS
CCAAATGCAGGTGCTTGCCT+TGG 0.504330 6:-18318859 MsG0680031330.01.T01:intron
ATTGACCGCAACCTAATGTT+GGG 0.512542 6:-18318434 MsG0680031330.01.T01:three_prime_UTR
TGTGTTTGTCCTAGTCCCCT+CGG 0.526819 6:-18318502 MsG0680031330.01.T01:three_prime_UTR
GGTTGCGGTCAATGTTCAAA+GGG 0.538514 6:+18318444 None:intergenic
GACTCTGCTAACTGCAATGG+TGG 0.541849 6:-18320712 MsG0680031330.01.T01:CDS
TACAGGAACACTTGGTTGAG+AGG 0.543267 6:-18318538 MsG0680031330.01.T01:CDS
ATGCAGGTGCTTGCCTTGGT+GGG 0.546695 6:-18318855 MsG0680031330.01.T01:intron
ATTCATCTTACAGGAACACT+TGG 0.548432 6:-18318546 MsG0680031330.01.T01:CDS
AGGTTGCGGTCAATGTTCAA+AGG 0.549088 6:+18318443 None:intergenic
GAAGATTGTTGAATTCAGTA+CGG 0.555167 6:-18321065 MsG0680031330.01.T01:intron
AGGGCAGCGTCAAGCTGTGA+TGG 0.566478 6:+18318463 None:intergenic
CATTGACCGCAACCTAATGT+TGG 0.566551 6:-18318435 MsG0680031330.01.T01:three_prime_UTR
TGTATGGATGAAAAGAGTTA+TGG 0.567300 6:+18318243 None:intergenic
TATGTTAATTTCGTCACTCG+AGG 0.571286 6:-18318350 MsG0680031330.01.T01:three_prime_UTR
TAGATACCATGAAGCAGTCT+CGG 0.588214 6:-18321950 None:intergenic
GTTGGAATAGGCAAAGAAGA+AGG 0.590955 6:+18320919 None:intergenic
TATGGTCCTTTCCTTCAGCA+AGG 0.606905 6:+18318261 None:intergenic
TACCATGAAGCAGTCTCGGA+AGG 0.608228 6:-18321946 None:intergenic
AAGAAGAAGGACGAGCAAGA+TGG 0.610167 6:+18320932 None:intergenic
AAGGACTCTGCTAACTGCAA+TGG 0.616200 6:-18320715 MsG0680031330.01.T01:CDS
ATCCAATTGGTCACCCACCA+AGG 0.621634 6:+18318842 None:intergenic
AGGAATTCGTCCTTTATGGG+AGG 0.639812 6:-18320875 MsG0680031330.01.T01:intron
GATGCCAACCTGATGATCAG+AGG 0.659266 6:+18318743 None:intergenic
AAACACCCAACATTAGGTTG+CGG 0.666936 6:+18318429 None:intergenic
TATACAATAGCATCAACCGA+GGG 0.675343 6:+18318486 None:intergenic
CGTCACCTAAAGCTTCCTCA+CGG 0.685945 6:-18318613 MsG0680031330.01.T01:CDS
GTATACAATAGCATCAACCG+AGG 0.719711 6:+18318485 None:intergenic
TCTCGCAGAGAAGCATCATG+CGG 0.719756 6:+18318571 None:intergenic
AATTCGAAACCAATCATACG+AGG 0.732933 6:-18321098 MsG0680031330.01.T01:CDS
ATACAATAGCATCAACCGAG+GGG 0.752964 6:+18318487 None:intergenic
TACTCCATAACATAGCCGTG+AGG 0.757014 6:+18318598 None:intergenic
ACTAAGCATTCGATTCAACG+AGG 0.817685 6:-18318793 MsG0680031330.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTATTTATGTTAGTTA+TGG - Chr6:18321184-18321203 MsG0680031330.01.T01:intron 10.0%
!!! ATATTTTTATTACCTTTATT+TGG - Chr6:18321111-18321130 MsG0680031330.01.T01:CDS 10.0%
!! TTAAGATAAAAATGTAGTAT+AGG - Chr6:18320128-18320147 MsG0680031330.01.T01:intron 15.0%
!!! TAAAAATATGTTTTTGTCTT+CGG + Chr6:18319887-18319906 None:intergenic 15.0%
!!! TGAAGTTTTTATTTTAGTTT+TGG - Chr6:18318821-18318840 MsG0680031330.01.T01:CDS 15.0%
!!! TGACAATTATTTTTGTTAAT+AGG - Chr6:18319212-18319231 MsG0680031330.01.T01:intron 15.0%
!! AAATCACACATAATAACAAT+AGG - Chr6:18318378-18318397 MsG0680031330.01.T01:three_prime_UTR 20.0%
!! AAATTTCACTAAAAGATCAA+TGG + Chr6:18320535-18320554 None:intergenic 20.0%
!! ATATGTTTGATGTATTGTAT+TGG - Chr6:18319152-18319171 MsG0680031330.01.T01:intron 20.0%
!! ATGTATATTGCACAAAAAAT+AGG + Chr6:18320331-18320350 None:intergenic 20.0%
!! ATTAACATATATATGATGCA+TGG + Chr6:18320398-18320417 None:intergenic 20.0%
!! CTTAATGCTTGAATATTATT+GGG - Chr6:18318924-18318943 MsG0680031330.01.T01:intron 20.0%
!! TCTTAATGCTTGAATATTAT+TGG - Chr6:18318923-18318942 MsG0680031330.01.T01:intron 20.0%
!! TTTAAGTATAAATAGGAGAA+GGG + Chr6:18318518-18318537 None:intergenic 20.0%
!!! AAATCTATTTGTTATTGAAC+CGG + Chr6:18318300-18318319 None:intergenic 20.0%
!!! AAATGGTTTTACACAAAATT+TGG - Chr6:18319671-18319690 MsG0680031330.01.T01:intron 20.0%
!!! TATTTTAGTTTTGGATGAAT+TGG - Chr6:18318830-18318849 MsG0680031330.01.T01:CDS 20.0%
!!! TATTTTTGTTAATAGGTTGA+AGG - Chr6:18319219-18319238 MsG0680031330.01.T01:intron 20.0%
!!! TTAGTGAAAATATTTTCATG+AGG - Chr6:18319559-18319578 MsG0680031330.01.T01:intron 20.0%
!!! TTTTAAGTATAAATAGGAGA+AGG + Chr6:18318519-18318538 None:intergenic 20.0%
!!! TTTTTGTGCAATATACATTT+TGG - Chr6:18320333-18320352 MsG0680031330.01.T01:intron 20.0%
! AAAAACCAGGAATGTTATTA+AGG + Chr6:18321522-18321541 None:intergenic 25.0%
! AACTTAAAAACAGAGCAAAA+AGG + Chr6:18321165-18321184 None:intergenic 25.0%
! AATATATAATGCACACGTAT+AGG + Chr6:18321484-18321503 None:intergenic 25.0%
! AGTAAAAAAAAAAAGTTGCC+TGG - Chr6:18320565-18320584 MsG0680031330.01.T01:intron 25.0%
! ATAGTCTAATTTCTAAACTG+AGG + Chr6:18320869-18320888 None:intergenic 25.0%
! ATATATAATGCACACGTATA+GGG + Chr6:18321483-18321502 None:intergenic 25.0%
! CTAGAATATATAAACTGGAA+TGG + Chr6:18319914-18319933 None:intergenic 25.0%
! GCATTATGAATCTATAAATC+AGG + Chr6:18319969-18319988 None:intergenic 25.0%
! TAATAACTCCAACAATACAT+TGG + Chr6:18320447-18320466 None:intergenic 25.0%
! TCAACAATCAACACAAATAA+AGG + Chr6:18320200-18320219 None:intergenic 25.0%
! TGATAATTTATGAGTTGTTC+TGG - Chr6:18319383-18319402 MsG0680031330.01.T01:intron 25.0%
! TGATGTATTGTATTGGATTT+AGG - Chr6:18319159-18319178 MsG0680031330.01.T01:intron 25.0%
! TTTGGAAGTTAACATCAAAA+AGG - Chr6:18321061-18321080 MsG0680031330.01.T01:intron 25.0%
!! ACATTTCTGTTGTTTATTGT+TGG - Chr6:18321281-18321300 MsG0680031330.01.T01:intron 25.0%
!! ATGGATTATACGATTCAAAA+AGG - Chr6:18319506-18319525 MsG0680031330.01.T01:intron 25.0%
!! CATTAATTTTAAACGTCTTC+TGG - Chr6:18320592-18320611 MsG0680031330.01.T01:intron 25.0%
!! GTTTAAAATTAATGAAGACC+AGG + Chr6:18320586-18320605 None:intergenic 25.0%
!! TAGTTTTTATATTTCCTGTG+TGG - Chr6:18321025-18321044 MsG0680031330.01.T01:intron 25.0%
!! TTTTCAGTAACTTCATATAG+AGG + Chr6:18320074-18320093 None:intergenic 25.0%
!!! ACACTCTAGAATATATAAAC+TGG + Chr6:18319919-18319938 None:intergenic 25.0%
!!! CAGAAATGTAATTTTAATCC+AGG + Chr6:18321270-18321289 None:intergenic 25.0%
!!! TATTGTCAAGCAGTTATTTT+AGG + Chr6:18318607-18318626 None:intergenic 25.0%
!!! TTAATAGGTTGAAGGATTTT+GGG - Chr6:18319227-18319246 MsG0680031330.01.T01:intron 25.0%
!!! TTCAAATTTGTTGCTCTAAA+TGG - Chr6:18319654-18319673 MsG0680031330.01.T01:intron 25.0%
!!! TTTTGGTTGATTTATTGTGT+TGG - Chr6:18319017-18319036 MsG0680031330.01.T01:intron 25.0%
AACAACTTCTAACTGCTTAT+TGG - Chr6:18320988-18321007 MsG0680031330.01.T01:intron 30.0%
AACTTCAAAAGTCAAATTGC+AGG + Chr6:18318808-18318827 None:intergenic 30.0%
AATAGGCTAATTGTTCTTCA+AGG - Chr6:18318395-18318414 MsG0680031330.01.T01:three_prime_UTR 30.0%
AATGCTTGAATATTATTGGG+CGG - Chr6:18318927-18318946 MsG0680031330.01.T01:intron 30.0%
ATCAAAAAGGAAAACTGTCA+TGG - Chr6:18321074-18321093 MsG0680031330.01.T01:CDS 30.0%
ATGAAGAGGAAATATTTGCA+TGG + Chr6:18320646-18320665 None:intergenic 30.0%
CGAATATTCTATCATTGACA+TGG + Chr6:18319799-18319818 None:intergenic 30.0%
CTTCACTAATGACTAAACAA+CGG + Chr6:18320802-18320821 None:intergenic 30.0%
GAAGATTGTTGAATTCAGTA+CGG - Chr6:18319127-18319146 MsG0680031330.01.T01:intron 30.0%
GAAGTTATTGAGTGGTTATT+GGG + Chr6:18318473-18318492 None:intergenic 30.0%
GAGAGATTACAAATGGTAAT+TGG + Chr6:18321215-18321234 None:intergenic 30.0%
GATTATGGTGATAACATTTG+TGG - Chr6:18321370-18321389 MsG0680031330.01.T01:intron 30.0%
GGTGATGAGATTAAGATTTA+GGG - Chr6:18318729-18318748 MsG0680031330.01.T01:intron 30.0%
GTTTACATCATGTAATGAAG+AGG + Chr6:18320660-18320679 None:intergenic 30.0%
TAATGTTATCCTCGTATGAT+TGG + Chr6:18319106-18319125 None:intergenic 30.0%
TAATTTATGAGTTGTTCTGG+AGG - Chr6:18319386-18319405 MsG0680031330.01.T01:intron 30.0%
TGCTTAGGATTTCTATGTAT+AGG - Chr6:18320758-18320777 MsG0680031330.01.T01:intron 30.0%
TGGCTTATGTTTCAATTTCA+TGG - Chr6:18319357-18319376 MsG0680031330.01.T01:intron 30.0%
TGTTATCACCATAATCCAAT+TGG + Chr6:18321366-18321385 None:intergenic 30.0%
TTCACTGTATAATATGCACT+AGG - Chr6:18320824-18320843 MsG0680031330.01.T01:intron 30.0%
TTTAGATCAACAAAATCACG+TGG + Chr6:18321140-18321159 None:intergenic 30.0%
TTTGTCTTCGGTTTCAATTA+TGG + Chr6:18319875-18319894 None:intergenic 30.0%
! AAAGTCAAATTGCAGGATTT+TGG + Chr6:18318801-18318820 None:intergenic 30.0%
! AAGTCAAATTGCAGGATTTT+GGG + Chr6:18318800-18318819 None:intergenic 30.0%
! AATTTATGAGTTGTTCTGGA+GGG - Chr6:18319387-18319406 MsG0680031330.01.T01:intron 30.0%
! AGTGTTCTAAACAAATGAGA+GGG + Chr6:18320944-18320963 None:intergenic 30.0%
! ATCAGAGCAAATTTGTGTTT+TGG - Chr6:18319051-18319070 MsG0680031330.01.T01:intron 30.0%
! GTCTTCAACAAGTCTATTTT+TGG - Chr6:18320291-18320310 MsG0680031330.01.T01:intron 30.0%
! GTTGGGAAAATGTTTTTCTA+TGG + Chr6:18320268-18320287 None:intergenic 30.0%
! TGGTGATGAGATTAAGATTT+AGG - Chr6:18318728-18318747 MsG0680031330.01.T01:intron 30.0%
! TGTGCAATATACATTTTGGA+AGG - Chr6:18320337-18320356 MsG0680031330.01.T01:intron 30.0%
! TGTTGATTGTTGAATTTGTG+GGG - Chr6:18320207-18320226 MsG0680031330.01.T01:intron 30.0%
!! AATGAGATGTTTGCTGTATT+GGG - Chr6:18318858-18318877 MsG0680031330.01.T01:intron 30.0%
!! GTGCAATATACATTTTGGAA+GGG - Chr6:18320338-18320357 MsG0680031330.01.T01:intron 30.0%
!! GTGTTGATTGTTGAATTTGT+GGG - Chr6:18320206-18320225 MsG0680031330.01.T01:intron 30.0%
!! GTTAATAGGTTGAAGGATTT+TGG - Chr6:18319226-18319245 MsG0680031330.01.T01:intron 30.0%
!! TGTGTTGATTGTTGAATTTG+TGG - Chr6:18320205-18320224 MsG0680031330.01.T01:intron 30.0%
!!! TTACTGTGTGACTTTTTTGA+AGG - Chr6:18319458-18319477 MsG0680031330.01.T01:intron 30.0%
AAAAGCAGCCAATGTATTGT+TGG - Chr6:18320436-18320455 MsG0680031330.01.T01:intron 35.0%
AAGAAGGAATTCGTCCTTTA+TGG - Chr6:18319313-18319332 MsG0680031330.01.T01:intron 35.0%
AAGCAACGAGACAAACATTT+CGG + Chr6:18319848-18319867 None:intergenic 35.0%
AATTCGAAACCAATCATACG+AGG - Chr6:18319094-18319113 MsG0680031330.01.T01:intron 35.0%
ACTCGCAATTTGGACAAAAA+TGG + Chr6:18320682-18320701 None:intergenic 35.0%
AGAAGGAATTCGTCCTTTAT+GGG - Chr6:18319314-18319333 MsG0680031330.01.T01:intron 35.0%
AGATGCAAATCTGTTGGAAT+AGG + Chr6:18319288-18319307 None:intergenic 35.0%
AGGTAGATACTTAATGGCTT+TGG - Chr6:18319337-18319356 MsG0680031330.01.T01:intron 35.0%
ATAAACTCCTGCAGTGATTA+TGG - Chr6:18319598-18319617 MsG0680031330.01.T01:intron 35.0%
ATAAAGGACGAATTCCTTCT+TGG + Chr6:18319314-18319333 None:intergenic 35.0%
ATCACGTGGAAACCAAATAA+AGG + Chr6:18321126-18321145 None:intergenic 35.0%
ATCCTAAGCACTAATTCAAC+AGG + Chr6:18320748-18320767 None:intergenic 35.0%
ATGTATAGGACTTCTCTAAG+TGG - Chr6:18320897-18320916 MsG0680031330.01.T01:CDS 35.0%
ATTCATCTTACAGGAACACT+TGG - Chr6:18321646-18321665 MsG0680031330.01.T01:intron 35.0%
CAAGCAAGAGAGATTACAAA+TGG + Chr6:18321222-18321241 None:intergenic 35.0%
CAGTAACTTCATATAGAGGA+GGG + Chr6:18320070-18320089 None:intergenic 35.0%
CATATGTTAGACTTCAGATC+AGG + Chr6:18318655-18318674 None:intergenic 35.0%
CATTTGTAATCTCTCTTGCT+TGG - Chr6:18321220-18321239 MsG0680031330.01.T01:intron 35.0%
GAATTCGATCTCATCATCTA+TGG - Chr6:18321244-18321263 MsG0680031330.01.T01:intron 35.0%
GACTATTCTACTCGATGTAT+AGG - Chr6:18320883-18320902 MsG0680031330.01.T01:CDS 35.0%
GATGTTAACTTCCAAAGAAC+CGG + Chr6:18321057-18321076 None:intergenic 35.0%
GGAAGTTATTGAGTGGTTAT+TGG + Chr6:18318474-18318493 None:intergenic 35.0%
GTGGAATGAATTCTACTACT+TGG - Chr6:18320916-18320935 MsG0680031330.01.T01:CDS 35.0%
TAAGTATCTACCTCCCATAA+AGG + Chr6:18319330-18319349 None:intergenic 35.0%
TATACAATAGCATCAACCGA+GGG + Chr6:18321709-18321728 None:intergenic 35.0%
TATGGGAGGTAGATACTTAA+TGG - Chr6:18319331-18319350 MsG0680031330.01.T01:intron 35.0%
TATGTTAATTTCGTCACTCG+AGG - Chr6:18321842-18321861 MsG0680031330.01.T01:intron 35.0%
TATTAGTTCATGCCAAATGC+AGG - Chr6:18321321-18321340 MsG0680031330.01.T01:intron 35.0%
TATTGTCATCACGACAAACT+TGG - Chr6:18318692-18318711 MsG0680031330.01.T01:intron 35.0%
TCAGTAACTTCATATAGAGG+AGG + Chr6:18320071-18320090 None:intergenic 35.0%
TGGAAAAGATGCAAATCTGT+TGG + Chr6:18319294-18319313 None:intergenic 35.0%
! ACACAAATTTGCTCTGATCA+AGG + Chr6:18319049-18319068 None:intergenic 35.0%
! ACATTTTCCATAATCACTGC+AGG + Chr6:18319608-18319627 None:intergenic 35.0%
! AGTTGAACAGTTCAAGTTTG+GGG - Chr6:18320026-18320045 MsG0680031330.01.T01:intron 35.0%
! ATGCATGGATTAAAGATGCA+CGG + Chr6:18320383-18320402 None:intergenic 35.0%
! CAGTGTTCTAAACAAATGAG+AGG + Chr6:18320945-18320964 None:intergenic 35.0%
! CAGTTGAACAGTTCAAGTTT+GGG - Chr6:18320025-18320044 MsG0680031330.01.T01:intron 35.0%
! CTTGTCCATTTGAAACAGTT+GGG - Chr6:18320482-18320501 MsG0680031330.01.T01:intron 35.0%
! GATTTGCATCTTTTCCAAGA+AGG - Chr6:18319297-18319316 MsG0680031330.01.T01:intron 35.0%
! TATTTGAAATCCTCGTCCAT+AGG - Chr6:18319821-18319840 MsG0680031330.01.T01:intron 35.0%
! TCAGTTGAACAGTTCAAGTT+TGG - Chr6:18320024-18320043 MsG0680031330.01.T01:intron 35.0%
!! GAATGAGATGTTTGCTGTAT+TGG - Chr6:18318857-18318876 MsG0680031330.01.T01:intron 35.0%
!! TATGATTGGTTTCGAATTCC+AGG + Chr6:18319092-18319111 None:intergenic 35.0%
!! TGATACCAGTTGATAGATAC+CGG - Chr6:18318278-18318297 MsG0680031330.01.T01:three_prime_UTR 35.0%
!!! GGCTCTTGGTGAATTTTTTT+TGG - Chr6:18319000-18319019 MsG0680031330.01.T01:intron 35.0%
AACCTAATGTTGGGTGTTTC+AGG - Chr6:18321767-18321786 MsG0680031330.01.T01:intron 40.0%
AACTCTCGAACATTTGGGAA+AGG + Chr6:18318442-18318461 None:intergenic 40.0%
ACCATCCCAACTGTTTCAAA+TGG + Chr6:18320490-18320509 None:intergenic 40.0%
ACTAAGCATTCGATTCAACG+AGG - Chr6:18321399-18321418 MsG0680031330.01.T01:intron 40.0%
ACTAATTCAACAGGCAGAAG+GGG + Chr6:18320739-18320758 None:intergenic 40.0%
ACTCTCGAACATTTGGGAAA+GGG + Chr6:18318441-18318460 None:intergenic 40.0%
AGACAAACATTTCGGACCTA+TGG + Chr6:18319840-18319859 None:intergenic 40.0%
ATACAATAGCATCAACCGAG+GGG + Chr6:18321708-18321727 None:intergenic 40.0%
ATGGTCTGGAAGTTATTGAG+TGG + Chr6:18318481-18318500 None:intergenic 40.0%
ATTGACCGCAACCTAATGTT+GGG - Chr6:18321758-18321777 MsG0680031330.01.T01:intron 40.0%
ATTTCCTGTGTGGATAATGC+CGG - Chr6:18321035-18321054 MsG0680031330.01.T01:intron 40.0%
CAAGACTCATCCTACTAGAT+GGG + Chr6:18319745-18319764 None:intergenic 40.0%
CACTAATTCAACAGGCAGAA+GGG + Chr6:18320740-18320759 None:intergenic 40.0%
CCAACTGTTTCAAATGGACA+AGG + Chr6:18320484-18320503 None:intergenic 40.0%
CCAGGAATGTTATTAAGGAC+AGG + Chr6:18321517-18321536 None:intergenic 40.0%
CCTGTCCTTAATAACATTCC+TGG - Chr6:18321514-18321533 MsG0680031330.01.T01:intron 40.0%
CTAGCTGTTCCAATTGACAT+CGG + Chr6:18320176-18320195 None:intergenic 40.0%
GTATACAATAGCATCAACCG+AGG + Chr6:18321710-18321729 None:intergenic 40.0%
GTTGGAATAGGCAAAGAAGA+AGG + Chr6:18319276-18319295 None:intergenic 40.0%
TAATGCTGTTCATAGTGCAC+TGG - Chr6:18319983-18320002 MsG0680031330.01.T01:intron 40.0%
TAGCTGTTCCAATTGACATC+GGG + Chr6:18320175-18320194 None:intergenic 40.0%
TCAGGAACCAAAACTGAATG+TGG + Chr6:18318637-18318656 None:intergenic 40.0%
TGCCTGTTGAATTAGTGCTT+AGG - Chr6:18320743-18320762 MsG0680031330.01.T01:intron 40.0%
TGCGAGACAATTCATCTTAC+AGG - Chr6:18321637-18321656 MsG0680031330.01.T01:intron 40.0%
TGGGGTGGTGAAAAGTTTAA+AGG - Chr6:18320044-18320063 MsG0680031330.01.T01:intron 40.0%
TTCCCAAATGTTCGAGAGTT+GGG - Chr6:18318442-18318461 MsG0680031330.01.T01:three_prime_UTR 40.0%
TTGAACCGGTATCTATCAAC+TGG + Chr6:18318286-18318305 None:intergenic 40.0%
TTTCCCAAATGTTCGAGAGT+TGG - Chr6:18318441-18318460 MsG0680031330.01.T01:three_prime_UTR 40.0%
! AAACACCCAACATTAGGTTG+CGG + Chr6:18321766-18321785 None:intergenic 40.0%
! AAGGGACTTCAAGGTTCTTT+TGG - Chr6:18320356-18320375 MsG0680031330.01.T01:intron 40.0%
! AGGGACTTCAAGGTTCTTTT+GGG - Chr6:18320357-18320376 MsG0680031330.01.T01:intron 40.0%
! CCTTGTCCATTTGAAACAGT+TGG - Chr6:18320481-18320500 MsG0680031330.01.T01:intron 40.0%
! CTAACTGCAATGGTGGAAAA+TGG - Chr6:18319487-18319506 MsG0680031330.01.T01:intron 40.0%
! GAAACTGCCACATTCAGTTT+TGG - Chr6:18318627-18318646 MsG0680031330.01.T01:CDS 40.0%
! TCAAATTGCAGGATTTTGGG+AGG + Chr6:18318797-18318816 None:intergenic 40.0%
! TCCATTTGAAACAGTTGGGA+TGG - Chr6:18320486-18320505 MsG0680031330.01.T01:intron 40.0%
! TTTGAAACAGTTGGGATGGT+TGG - Chr6:18320490-18320509 MsG0680031330.01.T01:intron 40.0%
! TTTTCACCTTGCTGAAGGAA+AGG - Chr6:18321925-18321944 MsG0680031330.01.T01:intron 40.0%
!! ACATTTTGGAAGGGACTTCA+AGG - Chr6:18320347-18320366 MsG0680031330.01.T01:intron 40.0%
!! AGACTTGTTGAAGACAGTGT+TGG + Chr6:18320286-18320305 None:intergenic 40.0%
!! ATAGTGCACTGGTTTGATGT+TGG - Chr6:18319994-18320013 MsG0680031330.01.T01:intron 40.0%
!! GACTTGTTGAAGACAGTGTT+GGG + Chr6:18320285-18320304 None:intergenic 40.0%
!!! TTGTGTTTTGGTACACTCGT+CGG - Chr6:18319063-18319082 MsG0680031330.01.T01:intron 40.0%
AAGAAGAAGGACGAGCAAGA+TGG + Chr6:18319263-18319282 None:intergenic 45.0%
AAGGACTCTGCTAACTGCAA+TGG - Chr6:18319477-18319496 MsG0680031330.01.T01:intron 45.0%
AATGCCTCTGATCATCAGGT+TGG - Chr6:18321445-18321464 MsG0680031330.01.T01:intron 45.0%
AATGTTGGGTGTTTCAGGAC+TGG - Chr6:18321772-18321791 MsG0680031330.01.T01:intron 45.0%
ACTCATCCTACTAGATGGGA+AGG + Chr6:18319741-18319760 None:intergenic 45.0%
AGACCCAACTCTCGAACATT+TGG + Chr6:18318448-18318467 None:intergenic 45.0%
AGGAATTCGTCCTTTATGGG+AGG - Chr6:18319317-18319336 MsG0680031330.01.T01:intron 45.0%
AGGAGAAGGGTCAGACATAA+GGG + Chr6:18318505-18318524 None:intergenic 45.0%
AGGTTGCGGTCAATGTTCAA+AGG + Chr6:18321752-18321771 None:intergenic 45.0%
CAAATTGCGAGTAACCTCAC+TGG - Chr6:18320690-18320709 MsG0680031330.01.T01:CDS 45.0%
CAGTGAGGTTACTCGCAATT+TGG + Chr6:18320692-18320711 None:intergenic 45.0%
CATGTGAACCCGATGTCAAT+TGG - Chr6:18320164-18320183 MsG0680031330.01.T01:intron 45.0%
CATTGACCGCAACCTAATGT+TGG - Chr6:18321757-18321776 MsG0680031330.01.T01:intron 45.0%
CCAAGACTCATCCTACTAGA+TGG + Chr6:18319746-18319765 None:intergenic 45.0%
CCATCTAGTAGGATGAGTCT+TGG - Chr6:18319743-18319762 MsG0680031330.01.T01:intron 45.0%
CGATGTCAATTGGAACAGCT+AGG - Chr6:18320174-18320193 MsG0680031330.01.T01:intron 45.0%
CGATTCAAAAAGGCTGTCTC+AGG - Chr6:18319516-18319535 MsG0680031330.01.T01:intron 45.0%
CTCATCCTACTAGATGGGAA+GGG + Chr6:18319740-18319759 None:intergenic 45.0%
GACCCAACTCTCGAACATTT+GGG + Chr6:18318447-18318466 None:intergenic 45.0%
GCACTAATTCAACAGGCAGA+AGG + Chr6:18320741-18320760 None:intergenic 45.0%
GGTTGCGGTCAATGTTCAAA+GGG + Chr6:18321751-18321770 None:intergenic 45.0%
GTAGGATGAGTCTTGGTTGT+TGG - Chr6:18319750-18319769 MsG0680031330.01.T01:intron 45.0%
GTATGATTGCATGACCAGTG+AGG + Chr6:18320707-18320726 None:intergenic 45.0%
GTCCTGAAACACCCAACATT+AGG + Chr6:18321772-18321791 None:intergenic 45.0%
GTGGGTGACCAATTGGATTA+TGG - Chr6:18321355-18321374 MsG0680031330.01.T01:intron 45.0%
GTTTCAAATGGACAAGGAGC+AGG + Chr6:18320478-18320497 None:intergenic 45.0%
TACAGGAACACTTGGTTGAG+AGG - Chr6:18321654-18321673 MsG0680031330.01.T01:intron 45.0%
TACTCCATAACATAGCCGTG+AGG + Chr6:18321597-18321616 None:intergenic 45.0%
TAGGAGAAGGGTCAGACATA+AGG + Chr6:18318506-18318525 None:intergenic 45.0%
TATGGTCCTTTCCTTCAGCA+AGG + Chr6:18321934-18321953 None:intergenic 45.0%
TCATCCTACTAGATGGGAAG+GGG + Chr6:18319739-18319758 None:intergenic 45.0%
! AACTTGGCGCTCTTTGAAGA+TGG - Chr6:18318708-18318727 MsG0680031330.01.T01:intron 45.0%
! GTTTTTCACGCAGGCTGTAA+TGG - Chr6:18321536-18321555 MsG0680031330.01.T01:intron 45.0%
! TGAACAGTTCAAGTTTGGGG+TGG - Chr6:18320029-18320048 MsG0680031330.01.T01:intron 45.0%
!! ACATTCCTGGTTTTTCACGC+AGG - Chr6:18321527-18321546 MsG0680031330.01.T01:intron 45.0%
!! TATGAGTTGTTCTGGAGGGA+GGG - Chr6:18319391-18319410 MsG0680031330.01.T01:intron 45.0%
!! TGTGGATAATGCCGGTTCTT+TGG - Chr6:18321043-18321062 MsG0680031330.01.T01:intron 45.0%
!! TTATGAGTTGTTCTGGAGGG+AGG - Chr6:18319390-18319409 MsG0680031330.01.T01:intron 45.0%
!!! GTTGCTTTTCACCTTGCTGA+AGG - Chr6:18321920-18321939 MsG0680031330.01.T01:intron 45.0%
!!! TGTGTTTTGGTACACTCGTC+GGG - Chr6:18319064-18319083 MsG0680031330.01.T01:intron 45.0%
!!! TATATTTTTTAAGTATAAAT+AGG + Chr6:18318525-18318544 None:intergenic 5.0%
AAGGGTCAGACATAAGGGGA+TGG + Chr6:18318500-18318519 None:intergenic 50.0%
ACATTTCGGACCTATGGACG+AGG + Chr6:18319834-18319853 None:intergenic 50.0%
ACGAGGACATAGTCAGTGTC+TGG - Chr6:18321416-18321435 MsG0680031330.01.T01:intron 50.0%
AGAACCGGCATTATCCACAC+AGG + Chr6:18321042-18321061 None:intergenic 50.0%
ATCCAATTGGTCACCCACCA+AGG + Chr6:18321353-18321372 None:intergenic 50.0%
CATAGCCGTGAGGAAGCTTT+AGG + Chr6:18321587-18321606 None:intergenic 50.0%
CGATCTCATCATCTATGGCC+TGG - Chr6:18321249-18321268 MsG0680031330.01.T01:intron 50.0%
CGTCACCTAAAGCTTCCTCA+CGG - Chr6:18321579-18321598 MsG0680031330.01.T01:intron 50.0%
CTCTCTCTCCTTGTTGGGAA+GGG - Chr6:18318979-18318998 MsG0680031330.01.T01:intron 50.0%
CTTCAAGGTTTCGAGAGCCT+TGG - Chr6:18318410-18318429 MsG0680031330.01.T01:three_prime_UTR 50.0%
GACTCTGCTAACTGCAATGG+TGG - Chr6:18319480-18319499 MsG0680031330.01.T01:intron 50.0%
GATGCCAACCTGATGATCAG+AGG + Chr6:18321452-18321471 None:intergenic 50.0%
GCTTCCTCACGGCTATGTTA+TGG - Chr6:18321590-18321609 MsG0680031330.01.T01:intron 50.0%
GGAGAAGGGTCAGACATAAG+GGG + Chr6:18318504-18318523 None:intergenic 50.0%
GGATGAGTCTTGGTTGTTGG+TGG - Chr6:18319753-18319772 MsG0680031330.01.T01:intron 50.0%
TACAGCCTGCGTGAAAAACC+AGG + Chr6:18321535-18321554 None:intergenic 50.0%
TCAGACATAAGGGGATGGTC+TGG + Chr6:18318495-18318514 None:intergenic 50.0%
TCCCAACAAGGAGAGAGAGA+GGG + Chr6:18318978-18318997 None:intergenic 50.0%
TCGCAATGCCTCTGATCATC+AGG - Chr6:18321441-18321460 MsG0680031330.01.T01:intron 50.0%
TCTCTCTCTCCTTGTTGGGA+AGG - Chr6:18318978-18318997 MsG0680031330.01.T01:intron 50.0%
TGTGTTTGTCCTAGTCCCCT+CGG - Chr6:18321690-18321709 MsG0680031330.01.T01:intron 50.0%
TTCCCAACAAGGAGAGAGAG+AGG + Chr6:18318979-18318998 None:intergenic 50.0%
TTGACCCCTTCCCATCTAGT+AGG - Chr6:18319732-18319751 MsG0680031330.01.T01:intron 50.0%
TTTGGGAAAGGGAGAGACCA+AGG + Chr6:18318430-18318449 None:intergenic 50.0%
!! AGGCTGTCTCAGGTCGTTTT+TGG - Chr6:18319526-18319545 MsG0680031330.01.T01:intron 50.0%
!! TCTCGCAGAGAAGCATCATG+CGG + Chr6:18321624-18321643 None:intergenic 50.0%
!!! AGGTCGTTTTTGGGAGGATC+TGG - Chr6:18319536-18319555 MsG0680031330.01.T01:intron 50.0%
!!! GGCTGTCTCAGGTCGTTTTT+GGG - Chr6:18319527-18319546 MsG0680031330.01.T01:intron 50.0%
!!! TGTCTCAGGTCGTTTTTGGG+AGG - Chr6:18319530-18319549 MsG0680031330.01.T01:intron 50.0%
AATGCAGGTGCTTGCCTTGG+TGG - Chr6:18321336-18321355 MsG0680031330.01.T01:intron 55.0%
ACCAAGAGCCCTTCCCAACA+AGG + Chr6:18318990-18319009 None:intergenic 55.0%
ATGCAGGTGCTTGCCTTGGT+GGG - Chr6:18321337-18321356 MsG0680031330.01.T01:intron 55.0%
CAACAAGGAGAGAGAGAGGG+GGG + Chr6:18318975-18318994 None:intergenic 55.0%
CCAAATGCAGGTGCTTGCCT+TGG - Chr6:18321333-18321352 MsG0680031330.01.T01:intron 55.0%
CCAACAAGGAGAGAGAGAGG+GGG + Chr6:18318976-18318995 None:intergenic 55.0%
CCAAGGCAAGCACCTGCATT+TGG + Chr6:18321336-18321355 None:intergenic 55.0%
CCCAACAAGGAGAGAGAGAG+GGG + Chr6:18318977-18318996 None:intergenic 55.0%
CCCCTCTCTCTCTCCTTGTT+GGG - Chr6:18318974-18318993 MsG0680031330.01.T01:intron 55.0%
TAGCATCAACCGAGGGGACT+AGG + Chr6:18321702-18321721 None:intergenic 55.0%
TCCTTGTTGGGAAGGGCTCT+TGG - Chr6:18318986-18319005 MsG0680031330.01.T01:intron 55.0%
! TGCCTTGGTGGGTGACCAAT+TGG - Chr6:18321348-18321367 MsG0680031330.01.T01:intron 55.0%
!! TGTTCTGGAGGGAGGGAGAA+AGG - Chr6:18319398-18319417 MsG0680031330.01.T01:intron 55.0%
AGGGCAGCGTCAAGCTGTGA+TGG + Chr6:18321732-18321751 None:intergenic 60.0%
CCCCCTCTCTCTCTCCTTGT+TGG - Chr6:18318973-18318992 MsG0680031330.01.T01:intron 60.0%
TGGTACACTCGTCGGGTACC+TGG - Chr6:18319071-18319090 MsG0680031330.01.T01:intron 60.0%
CAAGGAGAGAGAGAGGGGGG+CGG + Chr6:18318972-18318991 None:intergenic 65.0%
AGAGAGAGAGGGGGGCGGTG+GGG + Chr6:18318967-18318986 None:intergenic 70.0%
GAGAGAGAGAGGGGGGCGGT+GGG + Chr6:18318968-18318987 None:intergenic 70.0%
GGAGAGAGAGAGGGGGGCGG+TGG + Chr6:18318969-18318988 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 18318250 18321964 18318250 ID=MsG0680031330.01;Name=MsG0680031330.01
Chr6 mRNA 18318250 18321964 18318250 ID=MsG0680031330.01.T01;Parent=MsG0680031330.01;Name=MsG0680031330.01.T01;_AED=0.46;_eAED=0.47;_QI=0|0.8|0.83|1|1|1|6|284|181
Chr6 exon 18318250 18318665 18318250 ID=MsG0680031330.01.T01:exon:1800;Parent=MsG0680031330.01.T01
Chr6 exon 18318752 18318871 18318752 ID=MsG0680031330.01.T01:exon:1799;Parent=MsG0680031330.01.T01
Chr6 exon 18320657 18320734 18320657 ID=MsG0680031330.01.T01:exon:1798;Parent=MsG0680031330.01.T01
Chr6 exon 18320876 18320980 18320876 ID=MsG0680031330.01.T01:exon:1797;Parent=MsG0680031330.01.T01
Chr6 exon 18321066 18321158 18321066 ID=MsG0680031330.01.T01:exon:1796;Parent=MsG0680031330.01.T01
Chr6 exon 18321947 18321964 18321947 ID=MsG0680031330.01.T01:exon:1795;Parent=MsG0680031330.01.T01
Chr6 CDS 18321947 18321964 18321947 ID=MsG0680031330.01.T01:cds;Parent=MsG0680031330.01.T01
Chr6 CDS 18321066 18321158 18321066 ID=MsG0680031330.01.T01:cds;Parent=MsG0680031330.01.T01
Chr6 CDS 18320876 18320980 18320876 ID=MsG0680031330.01.T01:cds;Parent=MsG0680031330.01.T01
Chr6 CDS 18320657 18320734 18320657 ID=MsG0680031330.01.T01:cds;Parent=MsG0680031330.01.T01
Chr6 CDS 18318752 18318871 18318752 ID=MsG0680031330.01.T01:cds;Parent=MsG0680031330.01.T01
Chr6 CDS 18318534 18318665 18318534 ID=MsG0680031330.01.T01:cds;Parent=MsG0680031330.01.T01
Chr6 three_prime_UTR 18318250 18318533 18318250 ID=MsG0680031330.01.T01:three_prime_utr;Parent=MsG0680031330.01.T01
Gene Sequence

>MsG0680031330.01.T01

ATGAAGCAGTCTCGGAAGAGCAAATTTGTGTTTTGGTACACTCGTCGGGTACCTGGAATTCGAAACCAATCATACGAGGATAACATTAAGAAGATTGTTGAATTCAGTACGGTTGAAGGATTTTGGGTCTGCTATTGCCATCTTGCTCGTCCTTCTTCTTTGCCTATTCCAACAGATTTGCATCTTTTCCAAGAAGGAATTCGTCCTTTATGGGAGGACTCTGCTAACTGCAATGGTGGAAAATGGATTATACGATTCAAAAAGGCTGTCTCAGGTCGTTTTTGGGAGGATCTGGTGCTTGCCTTGGTGGGTGACCAATTGGATTATGGTGATAACATTTGTGGTGCAGTACTAAGCATTCGATTCAACGAGGACATAGTCAGTGTCTGGAATCGCAATGCCTCTGATCATCAGGCTGTAATGGCTTTGAGAGATTCGATTAAGCGTCACCTAAAGCTTCCTCACGGCTATGTTATGGAGTACAAGCCGCATGATGCTTCTCTGCGAGACAATTCATCTTACAGGAACACTTGGTTGAGAGGCTAG
Protein sequence

>MsG0680031330.01.T01

MKQSRKSKFVFWYTRRVPGIRNQSYEDNIKKIVEFSTVEGFWVCYCHLARPSSLPIPTDLHLFQEGIRPLWEDSANCNGGKWIIRFKKAVSGRFWEDLVLALVGDQLDYGDNICGAVLSIRFNEDIVSVWNRNASDHQAVMALRDSIKRHLKLPHGYVMEYKPHDASLRDNSSYRNTWLRG*