Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031825.01.T01 | XP_013451651.1 | 96.296 | 54 | 2 | 0 | 1 | 54 | 1 | 54 | 7.25E-27 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031825.01.T01 | Q9ZNQ7 | 78.846 | 52 | 11 | 0 | 1 | 52 | 1 | 52 | 3.14E-23 | 85.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031825.01.T01 | A0A072U7E3 | 96.296 | 54 | 2 | 0 | 1 | 54 | 1 | 54 | 3.46e-27 | 101 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680031825.01 | MsG0780041454.01 | PPI |
| MsG0680031825.01 | MsG0780041454.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031825.01.T01 | MTR_6g033495 | 96.296 | 54 | 2 | 0 | 1 | 54 | 1 | 54 | 8.78e-31 | 101 |
| MsG0680031825.01.T01 | MTR_7g111380 | 63.462 | 52 | 19 | 0 | 1 | 52 | 1 | 52 | 1.13e-20 | 76.3 |
| MsG0680031825.01.T01 | MTR_7g111350 | 69.231 | 52 | 16 | 0 | 1 | 52 | 1 | 52 | 1.57e-20 | 75.9 |
| MsG0680031825.01.T01 | MTR_7g111450 | 61.538 | 52 | 20 | 0 | 1 | 52 | 1 | 52 | 1.80e-19 | 73.2 |
| MsG0680031825.01.T01 | MTR_4g130660 | 55.769 | 52 | 22 | 1 | 3 | 53 | 5 | 56 | 6.90e-14 | 59.7 |
| MsG0680031825.01.T01 | MTR_4g130675 | 53.846 | 52 | 23 | 1 | 3 | 53 | 5 | 56 | 2.17e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031825.01.T01 | AT2G38905 | 87.037 | 54 | 7 | 0 | 1 | 54 | 1 | 54 | 1.24e-27 | 94.0 |
| MsG0680031825.01.T01 | AT3G05880 | 78.846 | 52 | 11 | 0 | 1 | 52 | 1 | 52 | 3.20e-24 | 85.1 |
| MsG0680031825.01.T01 | AT3G05890 | 65.385 | 52 | 18 | 0 | 1 | 52 | 1 | 52 | 1.95e-21 | 78.2 |
| MsG0680031825.01.T01 | AT1G57550 | 67.308 | 52 | 15 | 1 | 1 | 52 | 1 | 50 | 1.39e-17 | 68.6 |
| MsG0680031825.01.T01 | AT4G30650 | 64.706 | 51 | 17 | 1 | 4 | 53 | 6 | 56 | 1.26e-15 | 63.9 |
| MsG0680031825.01.T01 | AT4G30660 | 62.264 | 53 | 19 | 1 | 2 | 53 | 4 | 56 | 6.82e-15 | 62.0 |
| MsG0680031825.01.T01 | AT4G30660 | 62.264 | 53 | 19 | 1 | 2 | 53 | 4 | 56 | 6.82e-15 | 62.0 |
| MsG0680031825.01.T01 | AT2G24040 | 58.491 | 53 | 21 | 1 | 2 | 53 | 4 | 56 | 1.11e-14 | 61.6 |
| MsG0680031825.01.T01 | AT4G28088 | 56.604 | 53 | 22 | 1 | 2 | 53 | 4 | 56 | 1.23e-14 | 61.6 |
Find 14 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGTGGTGCTTACATTAA+TGG | 0.259918 | 6:-27473956 | MsG0680031825.01.T01:CDS |
| TTATCCAACTAATACATAAA+TGG | 0.327288 | 6:+27473909 | None:intergenic |
| TATGCCATTTATGTATTAGT+TGG | 0.343894 | 6:-27473913 | MsG0680031825.01.T01:CDS |
| GATGTGGTGCTTACATTAAT+GGG | 0.359212 | 6:-27473955 | MsG0680031825.01.T01:CDS |
| ATATAATGCAGGTGGAGTTC+TGG | 0.367696 | 6:-27473981 | MsG0680031825.01.T01:intron |
| ACATTAATGGGATACATTCC+AGG | 0.415500 | 6:-27473943 | MsG0680031825.01.T01:CDS |
| AATGGGTTCTGAAACATTTG+TGG | 0.467139 | 6:-27474948 | MsG0680031825.01.T01:CDS |
| TAACGAAGGAAAACACCAAC+AGG | 0.472138 | 6:+27474899 | None:intergenic |
| CATTAATGGGATACATTCCA+GGG | 0.506357 | 6:-27473942 | MsG0680031825.01.T01:CDS |
| TAAATGGCATATATAATCCC+TGG | 0.522842 | 6:+27473925 | None:intergenic |
| GCAATATTGTTACCTCCTGT+TGG | 0.523968 | 6:-27474914 | MsG0680031825.01.T01:CDS |
| GGTGGAGTTCTGGATAGATG+TGG | 0.667800 | 6:-27473971 | MsG0680031825.01.T01:CDS |
| CGAAGGAAAACACCAACAGG+AGG | 0.676364 | 6:+27474902 | None:intergenic |
| TCACTCCACAGCCATAACGA+AGG | 0.710773 | 6:+27474885 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCAAAAATGAATAAAGTA+TGG | + | Chr6:27474037-27474056 | None:intergenic | 15.0% |
| !! | TTATCCAACTAATACATAAA+TGG | + | Chr6:27474950-27474969 | None:intergenic | 20.0% |
| ! | CATTAACACAACATATCATA+AGG | + | Chr6:27474506-27474525 | None:intergenic | 25.0% |
| ! | GGTTTGTAATTATATAATGC+AGG | - | Chr6:27474864-27474883 | MsG0680031825.01.T01:intron | 25.0% |
| ! | TATATCGACAAAATCATATG+TGG | + | Chr6:27474361-27474380 | None:intergenic | 25.0% |
| ! | TTGTAATTATATAATGCAGG+TGG | - | Chr6:27474867-27474886 | MsG0680031825.01.T01:intron | 25.0% |
| ! | TTTGTCGAGTAAAAAAGAAT+GGG | - | Chr6:27474133-27474152 | MsG0680031825.01.T01:intron | 25.0% |
| !! | GATCTTCTGATTCATTTTAT+TGG | - | Chr6:27474843-27474862 | MsG0680031825.01.T01:intron | 25.0% |
| !! | TATGCCATTTATGTATTAGT+TGG | - | Chr6:27474943-27474962 | MsG0680031825.01.T01:CDS | 25.0% |
| !! | TTTTGTCGAGTAAAAAAGAA+TGG | - | Chr6:27474132-27474151 | MsG0680031825.01.T01:intron | 25.0% |
| ATTACACAACACATCATAAG+TGG | + | Chr6:27474703-27474722 | None:intergenic | 30.0% | |
| TAAATGGCATATATAATCCC+TGG | + | Chr6:27474934-27474953 | None:intergenic | 30.0% | |
| TTACTCGACAAAATCATATG+TGG | + | Chr6:27474125-27474144 | None:intergenic | 30.0% | |
| ACATTAATGGGATACATTCC+AGG | - | Chr6:27474913-27474932 | MsG0680031825.01.T01:CDS | 35.0% | |
| AGATGTGGTGCTTACATTAA+TGG | - | Chr6:27474900-27474919 | MsG0680031825.01.T01:CDS | 35.0% | |
| CATTAATGGGATACATTCCA+GGG | - | Chr6:27474914-27474933 | MsG0680031825.01.T01:CDS | 35.0% | |
| GATGTGGTGCTTACATTAAT+GGG | - | Chr6:27474901-27474920 | MsG0680031825.01.T01:CDS | 35.0% | |
| TCAATAATGTTACCTCCTGT+TGG | - | Chr6:27474180-27474199 | MsG0680031825.01.T01:intron | 35.0% | |
| ATATAATGCAGGTGGAGTTC+TGG | - | Chr6:27474875-27474894 | MsG0680031825.01.T01:intron | 40.0% | |
| ATCAAATGTCCGACACATGT+TGG | + | Chr6:27474542-27474561 | None:intergenic | 40.0% | |
| ATCTAATGTCCAACACGTGT+TGG | + | Chr6:27474747-27474766 | None:intergenic | 40.0% | |
| CATAACGAAGGAAAACCAAC+AGG | + | Chr6:27474198-27474217 | None:intergenic | 40.0% | |
| GCAATATTGTTACCTCCTGT+TGG | - | Chr6:27473942-27473961 | MsG0680031825.01.T01:CDS | 40.0% | |
| TAACGAAGGAAAACACCAAC+AGG | + | Chr6:27473960-27473979 | None:intergenic | 40.0% | |
| !! | GTTGGTGTTTTCCTTCGTTA+TGG | - | Chr6:27473960-27473979 | MsG0680031825.01.T01:CDS | 40.0% |
| !!! | CTGTTGGTTTTCCTTCGTTA+TGG | - | Chr6:27474196-27474215 | MsG0680031825.01.T01:intron | 40.0% |
| AACGAAGGAAAACCAACAGG+AGG | + | Chr6:27474195-27474214 | None:intergenic | 45.0% | |
| ACGTGAACACCAACATGTGT+CGG | - | Chr6:27474530-27474549 | MsG0680031825.01.T01:intron | 45.0% | |
| TCATGAACACCAACACGTGT+TGG | - | Chr6:27474735-27474754 | MsG0680031825.01.T01:intron | 45.0% | |
| ! | GTTTTCCTTCGTTATGGCTG+TGG | - | Chr6:27473966-27473985 | MsG0680031825.01.T01:CDS | 45.0% |
| CGAAGGAAAACACCAACAGG+AGG | + | Chr6:27473957-27473976 | None:intergenic | 50.0% | |
| TCACTCCACAGCCATAACGA+AGG | + | Chr6:27473974-27473993 | None:intergenic | 50.0% | |
| !! | GGTGGAGTTCTGGATAGATG+TGG | - | Chr6:27474885-27474904 | MsG0680031825.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 27473909 | 27474969 | 27473909 | ID=MsG0680031825.01;Name=MsG0680031825.01 |
| Chr6 | mRNA | 27473909 | 27474969 | 27473909 | ID=MsG0680031825.01.T01;Parent=MsG0680031825.01;Name=MsG0680031825.01.T01;_AED=0.43;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|54 |
| Chr6 | exon | 27473909 | 27473992 | 27473909 | ID=MsG0680031825.01.T01:exon:8643;Parent=MsG0680031825.01.T01 |
| Chr6 | exon | 27474889 | 27474969 | 27474889 | ID=MsG0680031825.01.T01:exon:8642;Parent=MsG0680031825.01.T01 |
| Chr6 | CDS | 27474889 | 27474969 | 27474889 | ID=MsG0680031825.01.T01:cds;Parent=MsG0680031825.01.T01 |
| Chr6 | CDS | 27473909 | 27473992 | 27473909 | ID=MsG0680031825.01.T01:cds;Parent=MsG0680031825.01.T01 |
| Gene Sequence |
| Protein sequence |
