Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031974.01.T01 | XP_024641717.1 | 81.716 | 443 | 58 | 3 | 1 | 420 | 56 | 498 | 0 | 697 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031974.01.T01 | Q9LYS2 | 45.169 | 445 | 208 | 8 | 5 | 420 | 42 | 479 | 2.65E-97 | 321 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031974.01.T01 | A0A072U9L8 | 81.716 | 443 | 58 | 3 | 1 | 420 | 56 | 498 | 0.0 | 697 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580029441.01 | MsG0680031974.01 | 0.800292 | 1.543208e-48 | 7.159329e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031974.01.T01 | MTR_6g034220 | 81.716 | 443 | 58 | 3 | 1 | 420 | 56 | 498 | 0.0 | 697 |
| MsG0680031974.01.T01 | MTR_6g034230 | 68.293 | 451 | 117 | 5 | 1 | 426 | 56 | 505 | 0.0 | 574 |
| MsG0680031974.01.T01 | MTR_0196s0020 | 65.333 | 450 | 132 | 4 | 1 | 426 | 56 | 505 | 0.0 | 560 |
| MsG0680031974.01.T01 | MTR_6g034265 | 65.991 | 444 | 126 | 5 | 1 | 420 | 55 | 497 | 0.0 | 555 |
| MsG0680031974.01.T01 | MTR_6g034310 | 62.640 | 447 | 130 | 6 | 6 | 426 | 56 | 491 | 7.92e-172 | 517 |
| MsG0680031974.01.T01 | MTR_6g034310 | 62.640 | 447 | 130 | 6 | 6 | 426 | 56 | 491 | 6.95e-171 | 517 |
| MsG0680031974.01.T01 | MTR_0019s0020 | 64.318 | 440 | 112 | 7 | 6 | 420 | 30 | 449 | 8.54e-169 | 501 |
| MsG0680031974.01.T01 | MTR_0019s0020 | 64.318 | 440 | 112 | 7 | 6 | 420 | 30 | 449 | 1.20e-167 | 500 |
| MsG0680031974.01.T01 | MTR_0019s0020 | 64.318 | 440 | 112 | 7 | 6 | 420 | 30 | 449 | 1.02e-165 | 499 |
| MsG0680031974.01.T01 | MTR_0019s0020 | 64.318 | 440 | 112 | 7 | 6 | 420 | 30 | 449 | 1.20e-164 | 499 |
| MsG0680031974.01.T01 | MTR_0019s0020 | 64.318 | 440 | 112 | 7 | 6 | 420 | 30 | 449 | 1.33e-164 | 499 |
| MsG0680031974.01.T01 | MTR_6g034755 | 62.150 | 428 | 136 | 5 | 19 | 420 | 79 | 506 | 4.21e-160 | 489 |
| MsG0680031974.01.T01 | MTR_1g099280 | 60.179 | 447 | 151 | 5 | 1 | 420 | 53 | 499 | 3.48e-158 | 480 |
| MsG0680031974.01.T01 | MTR_1g099280 | 60.179 | 447 | 151 | 5 | 1 | 420 | 53 | 499 | 1.01e-156 | 480 |
| MsG0680031974.01.T01 | MTR_1g099280 | 60.179 | 447 | 151 | 5 | 1 | 420 | 78 | 524 | 1.24e-156 | 480 |
| MsG0680031974.01.T01 | MTR_6g034350 | 60.880 | 432 | 143 | 5 | 15 | 420 | 75 | 506 | 3.55e-149 | 460 |
| MsG0680031974.01.T01 | MTR_6g034335 | 57.727 | 440 | 156 | 8 | 11 | 420 | 64 | 503 | 3.50e-144 | 446 |
| MsG0680031974.01.T01 | MTR_6g034270 | 67.774 | 301 | 74 | 3 | 143 | 420 | 16 | 316 | 2.00e-122 | 385 |
| MsG0680031974.01.T01 | MTR_8g040620 | 29.698 | 431 | 257 | 12 | 15 | 417 | 62 | 474 | 3.08e-39 | 151 |
| MsG0680031974.01.T01 | MTR_8g015980 | 30.024 | 423 | 252 | 12 | 21 | 417 | 67 | 471 | 1.08e-38 | 149 |
| MsG0680031974.01.T01 | MTR_6g084320 | 29.954 | 434 | 236 | 12 | 24 | 417 | 55 | 460 | 3.38e-38 | 147 |
| MsG0680031974.01.T01 | MTR_3g056705 | 29.333 | 300 | 185 | 6 | 145 | 417 | 254 | 553 | 5.32e-38 | 147 |
| MsG0680031974.01.T01 | MTR_6g084320 | 29.954 | 434 | 236 | 12 | 24 | 417 | 55 | 460 | 6.44e-38 | 147 |
| MsG0680031974.01.T01 | MTR_1g069450 | 31.183 | 279 | 165 | 4 | 168 | 420 | 245 | 522 | 3.82e-36 | 142 |
| MsG0680031974.01.T01 | MTR_7g098690 | 30.797 | 276 | 168 | 2 | 168 | 420 | 243 | 518 | 7.13e-36 | 141 |
| MsG0680031974.01.T01 | MTR_5g033030 | 28.655 | 342 | 202 | 9 | 114 | 417 | 160 | 497 | 3.52e-33 | 133 |
| MsG0680031974.01.T01 | MTR_5g094830 | 31.034 | 290 | 171 | 7 | 157 | 417 | 217 | 506 | 9.35e-33 | 132 |
| MsG0680031974.01.T01 | MTR_8g015970 | 31.667 | 300 | 174 | 5 | 144 | 417 | 1 | 295 | 1.31e-31 | 128 |
| MsG0680031974.01.T01 | MTR_8g015970 | 31.667 | 300 | 174 | 5 | 144 | 417 | 1 | 295 | 1.41e-31 | 128 |
| MsG0680031974.01.T01 | MTR_8g015970 | 32.847 | 274 | 157 | 4 | 170 | 417 | 27 | 299 | 1.44e-31 | 128 |
| MsG0680031974.01.T01 | MTR_8g015970 | 32.847 | 274 | 157 | 4 | 170 | 417 | 27 | 299 | 1.70e-31 | 128 |
| MsG0680031974.01.T01 | MTR_8g016000 | 32.482 | 274 | 158 | 8 | 170 | 417 | 37 | 309 | 1.79e-31 | 125 |
| MsG0680031974.01.T01 | MTR_3g056675 | 29.126 | 309 | 193 | 6 | 138 | 420 | 3 | 311 | 1.35e-29 | 122 |
| MsG0680031974.01.T01 | MTR_5g033320 | 27.027 | 296 | 191 | 6 | 147 | 417 | 196 | 491 | 3.10e-29 | 121 |
| MsG0680031974.01.T01 | MTR_3g056700 | 28.188 | 298 | 190 | 4 | 147 | 420 | 12 | 309 | 1.50e-28 | 119 |
| MsG0680031974.01.T01 | MTR_8g080050 | 28.364 | 275 | 164 | 6 | 172 | 420 | 196 | 463 | 1.74e-27 | 116 |
| MsG0680031974.01.T01 | MTR_8g061970 | 25.490 | 357 | 225 | 7 | 95 | 420 | 169 | 515 | 9.71e-25 | 107 |
| MsG0680031974.01.T01 | MTR_8g016070 | 26.055 | 403 | 234 | 14 | 58 | 417 | 100 | 481 | 1.04e-24 | 107 |
| MsG0680031974.01.T01 | MTR_8g040170 | 29.670 | 273 | 154 | 6 | 170 | 417 | 25 | 284 | 3.42e-24 | 106 |
| MsG0680031974.01.T01 | MTR_5g094810 | 28.667 | 300 | 166 | 8 | 147 | 417 | 211 | 491 | 4.93e-24 | 105 |
| MsG0680031974.01.T01 | MTR_6g034310 | 51.969 | 127 | 38 | 3 | 317 | 420 | 1 | 127 | 6.47e-23 | 102 |
| MsG0680031974.01.T01 | MTR_1g088680 | 28.212 | 358 | 213 | 12 | 94 | 417 | 179 | 526 | 6.49e-23 | 102 |
| MsG0680031974.01.T01 | MTR_2g436710 | 29.137 | 278 | 169 | 7 | 163 | 417 | 245 | 517 | 1.78e-22 | 100 |
| MsG0680031974.01.T01 | MTR_8g016020 | 30.175 | 285 | 143 | 8 | 170 | 428 | 10 | 264 | 3.63e-22 | 100 |
| MsG0680031974.01.T01 | MTR_2g436730 | 28.114 | 281 | 176 | 7 | 160 | 417 | 238 | 515 | 1.72e-21 | 98.2 |
| MsG0680031974.01.T01 | MTR_2g436680 | 25.765 | 392 | 253 | 9 | 57 | 417 | 133 | 517 | 3.13e-21 | 97.4 |
| MsG0680031974.01.T01 | MTR_2g019020 | 28.774 | 212 | 137 | 5 | 144 | 348 | 198 | 402 | 1.35e-17 | 86.3 |
| MsG0680031974.01.T01 | MTR_2g019020 | 28.774 | 212 | 137 | 5 | 144 | 348 | 198 | 402 | 1.68e-17 | 85.9 |
| MsG0680031974.01.T01 | MTR_2g019020 | 28.774 | 212 | 137 | 5 | 144 | 348 | 15 | 219 | 3.03e-17 | 85.1 |
| MsG0680031974.01.T01 | MTR_3g011820 | 27.922 | 308 | 194 | 11 | 45 | 339 | 138 | 430 | 4.46e-16 | 81.3 |
| MsG0680031974.01.T01 | MTR_8g105170 | 24.664 | 223 | 156 | 3 | 87 | 304 | 873 | 1088 | 1.10e-13 | 73.9 |
| MsG0680031974.01.T01 | MTR_3g011840 | 25.436 | 287 | 195 | 7 | 62 | 339 | 38 | 314 | 1.93e-13 | 73.2 |
| MsG0680031974.01.T01 | MTR_2g019020 | 29.341 | 167 | 113 | 3 | 183 | 348 | 3 | 165 | 3.96e-12 | 68.9 |
| MsG0680031974.01.T01 | MTR_8g009630 | 33.103 | 145 | 90 | 4 | 82 | 223 | 122 | 262 | 1.26e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031974.01.T01 | AT3G59140 | 45.169 | 445 | 208 | 8 | 5 | 420 | 42 | 479 | 2.70e-98 | 321 |
| MsG0680031974.01.T01 | AT3G13080 | 30.838 | 334 | 191 | 9 | 121 | 417 | 186 | 516 | 4.53e-38 | 147 |
| MsG0680031974.01.T01 | AT3G13080 | 30.838 | 334 | 191 | 9 | 121 | 417 | 186 | 516 | 4.53e-38 | 147 |
| MsG0680031974.01.T01 | AT3G13080 | 32.042 | 284 | 168 | 6 | 159 | 417 | 233 | 516 | 5.56e-38 | 147 |
| MsG0680031974.01.T01 | AT3G13080 | 32.042 | 284 | 168 | 6 | 159 | 417 | 233 | 516 | 5.85e-38 | 147 |
| MsG0680031974.01.T01 | AT3G13080 | 32.042 | 284 | 168 | 6 | 159 | 417 | 233 | 516 | 6.54e-38 | 147 |
| MsG0680031974.01.T01 | AT3G60160 | 29.966 | 297 | 184 | 4 | 148 | 420 | 213 | 509 | 6.86e-38 | 147 |
| MsG0680031974.01.T01 | AT3G60160 | 29.966 | 297 | 184 | 4 | 148 | 420 | 213 | 509 | 8.04e-38 | 147 |
| MsG0680031974.01.T01 | AT3G60160 | 29.966 | 297 | 184 | 4 | 148 | 420 | 177 | 473 | 9.54e-38 | 146 |
| MsG0680031974.01.T01 | AT1G04120 | 33.696 | 276 | 160 | 2 | 168 | 420 | 226 | 501 | 1.13e-37 | 146 |
| MsG0680031974.01.T01 | AT1G04120 | 33.696 | 276 | 160 | 2 | 168 | 420 | 226 | 501 | 1.24e-37 | 146 |
| MsG0680031974.01.T01 | AT1G04120 | 33.696 | 276 | 160 | 2 | 168 | 420 | 226 | 501 | 1.29e-37 | 146 |
| MsG0680031974.01.T01 | AT3G13090 | 28.030 | 396 | 230 | 13 | 62 | 417 | 97 | 477 | 4.07e-36 | 142 |
| MsG0680031974.01.T01 | AT3G13100 | 28.767 | 292 | 185 | 4 | 149 | 417 | 209 | 500 | 7.70e-34 | 135 |
| MsG0680031974.01.T01 | AT3G62700 | 28.689 | 366 | 220 | 9 | 82 | 417 | 161 | 515 | 3.24e-30 | 124 |
| MsG0680031974.01.T01 | AT3G21250 | 31.481 | 270 | 160 | 6 | 173 | 417 | 204 | 473 | 1.79e-27 | 116 |
| MsG0680031974.01.T01 | AT3G21250 | 31.481 | 270 | 160 | 6 | 173 | 417 | 204 | 473 | 1.82e-27 | 116 |
| MsG0680031974.01.T01 | AT3G21250 | 31.481 | 270 | 160 | 6 | 173 | 417 | 210 | 479 | 1.88e-27 | 116 |
| MsG0680031974.01.T01 | AT3G21250 | 31.481 | 270 | 160 | 6 | 173 | 417 | 210 | 479 | 1.93e-27 | 116 |
| MsG0680031974.01.T01 | AT3G13100 | 27.027 | 259 | 158 | 5 | 186 | 417 | 1 | 255 | 4.22e-25 | 108 |
| MsG0680031974.01.T01 | AT2G47800 | 26.975 | 367 | 233 | 8 | 88 | 426 | 172 | 531 | 2.32e-23 | 103 |
| MsG0680031974.01.T01 | AT1G30400 | 25.817 | 306 | 188 | 9 | 53 | 339 | 106 | 391 | 9.65e-16 | 80.5 |
| MsG0680031974.01.T01 | AT1G30400 | 25.817 | 306 | 188 | 9 | 53 | 339 | 106 | 391 | 9.65e-16 | 80.5 |
| MsG0680031974.01.T01 | AT2G34660 | 24.042 | 287 | 193 | 7 | 65 | 339 | 118 | 391 | 2.68e-14 | 75.9 |
| MsG0680031974.01.T01 | AT2G34660 | 24.042 | 287 | 193 | 7 | 65 | 339 | 118 | 391 | 2.68e-14 | 75.9 |
| MsG0680031974.01.T01 | AT1G30410 | 23.881 | 335 | 216 | 12 | 32 | 348 | 89 | 402 | 6.32e-13 | 71.6 |
| MsG0680031974.01.T01 | AT1G30410 | 23.881 | 335 | 216 | 12 | 32 | 348 | 89 | 402 | 6.32e-13 | 71.6 |
| MsG0680031974.01.T01 | AT1G30410 | 23.881 | 335 | 216 | 12 | 32 | 348 | 89 | 402 | 6.32e-13 | 71.6 |
| MsG0680031974.01.T01 | AT1G30410 | 23.881 | 335 | 216 | 12 | 32 | 348 | 89 | 402 | 7.07e-13 | 71.2 |
| MsG0680031974.01.T01 | AT1G30420 | 23.460 | 341 | 210 | 12 | 32 | 348 | 89 | 402 | 8.52e-13 | 70.9 |
| MsG0680031974.01.T01 | AT1G30420 | 23.754 | 341 | 209 | 13 | 32 | 348 | 89 | 402 | 1.27e-12 | 70.5 |
| MsG0680031974.01.T01 | AT1G30410 | 23.669 | 338 | 216 | 13 | 32 | 348 | 89 | 405 | 2.39e-12 | 69.7 |
| MsG0680031974.01.T01 | AT1G30410 | 24.590 | 305 | 194 | 11 | 62 | 348 | 1 | 287 | 3.54e-12 | 69.3 |
Find 73 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCTCATGTTCATTGTTTC+TGG | 0.064987 | 6:-31012751 | MsG0680031974.01.T01:CDS |
| GCGATAACCAATGGCTTTCT+AGG | 0.182514 | 6:-31012955 | MsG0680031974.01.T01:CDS |
| TTAGATATTCTATCGTTTCT+AGG | 0.200439 | 6:-31012664 | MsG0680031974.01.T01:CDS |
| AGCCTTTATGCTCCTTTAAA+TGG | 0.205493 | 6:-31012574 | MsG0680031974.01.T01:CDS |
| AAGAGTCCAGCTTTGGAAAA+AGG | 0.242038 | 6:+31012514 | None:intergenic |
| TCATTGAACTGACCATTTAA+AGG | 0.245071 | 6:+31012562 | None:intergenic |
| CAAACATGAAGGTTGTTTAT+TGG | 0.254849 | 6:-31012176 | MsG0680031974.01.T01:CDS |
| TGCATTTATCCTTAGGAGTT+TGG | 0.270345 | 6:-31012927 | MsG0680031974.01.T01:CDS |
| TTTAACAACCATTCGTTTAG+AGG | 0.272222 | 6:+31012865 | None:intergenic |
| ATCTTGCGCGACCATGAGTT+GGG | 0.306343 | 6:+31011779 | None:intergenic |
| GATTCTGAATGAATTTATAT+TGG | 0.311476 | 6:-31012221 | MsG0680031974.01.T01:CDS |
| TAAAGGCTTTCACCATTTAC+AGG | 0.355943 | 6:+31012589 | None:intergenic |
| CGGTCAAACACTCTTAACTT+TGG | 0.360778 | 6:+31012415 | None:intergenic |
| GGGTTGTTGCATTTATCCTT+AGG | 0.362749 | 6:-31012934 | MsG0680031974.01.T01:CDS |
| CTTTATTAGAAAGATTGATA+TGG | 0.363515 | 6:+31012153 | None:intergenic |
| GTTCTGAGGCTCTTGGACTA+TGG | 0.368491 | 6:-31011740 | MsG0680031974.01.T01:CDS |
| GGGTCAAGAGAAAACACTTT+TGG | 0.375068 | 6:-31012449 | MsG0680031974.01.T01:CDS |
| TGGTTGTTAAAACTCTTTCA+TGG | 0.375261 | 6:-31012853 | MsG0680031974.01.T01:CDS |
| AATGTTAAGTCTATCAAATT+CGG | 0.385611 | 6:-31012035 | MsG0680031974.01.T01:CDS |
| CTTGCTAAAGAGTCCAGCTT+TGG | 0.398363 | 6:+31012507 | None:intergenic |
| CGCGCAAGATGAGAGATTAA+AGG | 0.401577 | 6:-31011766 | MsG0680031974.01.T01:CDS |
| GACCATTTAAAGGAGCATAA+AGG | 0.402637 | 6:+31012572 | None:intergenic |
| GGTCAAACACTCTTAACTTT+GGG | 0.410044 | 6:+31012416 | None:intergenic |
| TGTGAAGTCGATCCTGTAAA+TGG | 0.414640 | 6:-31012601 | MsG0680031974.01.T01:CDS |
| GACTAGCAAACGCGCATCAT+TGG | 0.432051 | 6:-31011865 | MsG0680031974.01.T01:CDS |
| CATCTTGCGCGACCATGAGT+TGG | 0.434379 | 6:+31011778 | None:intergenic |
| TCTTTAGCAAGATCTCGTTT+TGG | 0.436139 | 6:-31012495 | MsG0680031974.01.T01:CDS |
| GTTGAATCCATTGATGAAAA+CGG | 0.440820 | 6:-31012470 | MsG0680031974.01.T01:CDS |
| TATCCGTGTCTTATGCGATT+GGG | 0.451343 | 6:-31012714 | MsG0680031974.01.T01:CDS |
| TATTTCCGCAGTAGACTTGT+TGG | 0.454292 | 6:-31012100 | MsG0680031974.01.T01:CDS |
| TAAGTTTATCTGTCAGCCTT+CGG | 0.473615 | 6:-31012813 | MsG0680031974.01.T01:CDS |
| AAGGCTAGTTCTGAGGCTCT+TGG | 0.474719 | 6:-31011747 | MsG0680031974.01.T01:CDS |
| TTGAATCCATTGATGAAAAC+GGG | 0.476454 | 6:-31012469 | MsG0680031974.01.T01:CDS |
| TTATCAATGCAATACACAAT+TGG | 0.478751 | 6:+31011906 | None:intergenic |
| CGTTTAGAGGAAAGACATCA+TGG | 0.487454 | 6:+31012878 | None:intergenic |
| CATGAAAGTTTCAAACATGA+AGG | 0.489149 | 6:-31012187 | MsG0680031974.01.T01:CDS |
| TTATCCGTGTCTTATGCGAT+TGG | 0.492322 | 6:-31012715 | MsG0680031974.01.T01:CDS |
| TCATGGACTAACGTGGTTGT+CGG | 0.494018 | 6:-31012836 | MsG0680031974.01.T01:CDS |
| AAAGTGTTGAAGCGATATGC+AGG | 0.497615 | 6:-31011717 | MsG0680031974.01.T01:CDS |
| CTCTAAAATCCAAACTCCTA+AGG | 0.499628 | 6:+31012918 | None:intergenic |
| AACTTACAAGAGCATGGTTG+TGG | 0.503807 | 6:-31012783 | MsG0680031974.01.T01:CDS |
| CGATAACCAATGGCTTTCTA+GGG | 0.517505 | 6:-31012954 | MsG0680031974.01.T01:CDS |
| GAATTTATATTGGTTGCTGA+AGG | 0.523275 | 6:-31012211 | MsG0680031974.01.T01:CDS |
| TCAATGCAATACACAATTGG+AGG | 0.532210 | 6:+31011909 | None:intergenic |
| AAAACCGACGAATGTGATGA+TGG | 0.532483 | 6:+31012334 | None:intergenic |
| TTAGTTTCTGCGATAACCAA+TGG | 0.533926 | 6:-31012964 | MsG0680031974.01.T01:CDS |
| AAGGTACTCACACTATCTTC+TGG | 0.545705 | 6:-31012250 | MsG0680031974.01.T01:CDS |
| TGTCATTCATCATGATCTTG+AGG | 0.546542 | 6:-31013035 | None:intergenic |
| TAGCAAACGCGCATCATTGG+CGG | 0.549928 | 6:-31011862 | MsG0680031974.01.T01:CDS |
| ATGAATAACAACTTTCTGCT+CGG | 0.553869 | 6:+31012395 | None:intergenic |
| TTGTGTTGTAAGTTTGCTAG+TGG | 0.563723 | 6:+31011810 | None:intergenic |
| ATGTCTTTCCTCTAAACGAA+TGG | 0.565641 | 6:-31012873 | MsG0680031974.01.T01:CDS |
| TTATCAAAATCTCTCTCTTG+TGG | 0.566243 | 6:+31012293 | None:intergenic |
| CAACAACCCTAGAAAGCCAT+TGG | 0.566389 | 6:+31012948 | None:intergenic |
| AATATCTAAAGCTGCCTCGA+GGG | 0.571494 | 6:+31012678 | None:intergenic |
| ATAAATTCATTCAGAATCAG+AGG | 0.586067 | 6:+31012226 | None:intergenic |
| CATCGTACTAGACTTCGAAA+AGG | 0.587274 | 6:+31013000 | None:intergenic |
| TAAAACAACTTACAAGAGCA+TGG | 0.596670 | 6:-31012789 | MsG0680031974.01.T01:CDS |
| CAAGTTTCAACCCCAACTCA+TGG | 0.599410 | 6:-31011790 | MsG0680031974.01.T01:CDS |
| TCGGCTAGATTGAATCACTC+TGG | 0.602605 | 6:-31012016 | MsG0680031974.01.T01:CDS |
| TGAACCATCATCACATTCGT+CGG | 0.607235 | 6:-31012338 | MsG0680031974.01.T01:CDS |
| AACTCTTTCATGGACTAACG+TGG | 0.617828 | 6:-31012843 | MsG0680031974.01.T01:CDS |
| AATCTATATCTCAAAGACAA+TGG | 0.620540 | 6:-31012123 | MsG0680031974.01.T01:CDS |
| TCTTGCGCGACCATGAGTTG+GGG | 0.637044 | 6:+31011780 | None:intergenic |
| GAGATTAAAGGCTAGTTCTG+AGG | 0.641445 | 6:-31011754 | MsG0680031974.01.T01:CDS |
| TAGACTTCGAAAAGGTCTCG+AGG | 0.650682 | 6:+31013008 | None:intergenic |
| GAATATCTAAAGCTGCCTCG+AGG | 0.657158 | 6:+31012677 | None:intergenic |
| GCTCCCAATCGCATAAGACA+CGG | 0.664638 | 6:+31012711 | None:intergenic |
| AAGTGTTGAAGCGATATGCA+GGG | 0.667791 | 6:-31011716 | MsG0680031974.01.T01:CDS |
| GCTAGATTGAATCACTCTGG+TGG | 0.679181 | 6:-31012013 | MsG0680031974.01.T01:intron |
| CGTTTGCTAGTCCAATTGTG+CGG | 0.695014 | 6:+31011876 | None:intergenic |
| TCACTCCAACAAGTCTACTG+CGG | 0.695077 | 6:+31012095 | None:intergenic |
| GAGCAGTGAACTGTCCCTCG+AGG | 0.708706 | 6:-31012692 | MsG0680031974.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTAACATTTTTTTATAAG+TGG | + | Chr6:31012671-31012690 | None:intergenic | 15.0% |
| !! | AATAATGAATTATGTTATCG+TGG | - | Chr6:31012729-31012748 | MsG0680031974.01.T01:CDS | 20.0% |
| !! | AATGTTAAGTCTATCAAATT+CGG | - | Chr6:31012681-31012700 | MsG0680031974.01.T01:CDS | 20.0% |
| !! | CTTTATTAGAAAGATTGATA+TGG | + | Chr6:31012566-31012585 | None:intergenic | 20.0% |
| !! | GATTCTGAATGAATTTATAT+TGG | - | Chr6:31012495-31012514 | MsG0680031974.01.T01:CDS | 20.0% |
| ! | AATCTATATCTCAAAGACAA+TGG | - | Chr6:31012593-31012612 | MsG0680031974.01.T01:CDS | 25.0% |
| ! | ATAAATTCATTCAGAATCAG+AGG | + | Chr6:31012493-31012512 | None:intergenic | 25.0% |
| ! | TTAGATATTCTATCGTTTCT+AGG | - | Chr6:31012052-31012071 | MsG0680031974.01.T01:CDS | 25.0% |
| ! | TTATCAATGCAATACACAAT+TGG | + | Chr6:31012813-31012832 | None:intergenic | 25.0% |
| ATGAATAACAACTTTCTGCT+CGG | + | Chr6:31012324-31012343 | None:intergenic | 30.0% | |
| CAAACATGAAGGTTGTTTAT+TGG | - | Chr6:31012540-31012559 | MsG0680031974.01.T01:CDS | 30.0% | |
| CATGAAAGTTTCAAACATGA+AGG | - | Chr6:31012529-31012548 | MsG0680031974.01.T01:CDS | 30.0% | |
| GTTGAATCCATTGATGAAAA+CGG | - | Chr6:31012246-31012265 | MsG0680031974.01.T01:CDS | 30.0% | |
| TAAAACAACTTACAAGAGCA+TGG | - | Chr6:31011927-31011946 | MsG0680031974.01.T01:intron | 30.0% | |
| TCATTGAACTGACCATTTAA+AGG | + | Chr6:31012157-31012176 | None:intergenic | 30.0% | |
| TGGTTGTTAAAACTCTTTCA+TGG | - | Chr6:31011863-31011882 | MsG0680031974.01.T01:CDS | 30.0% | |
| TTATCAAAATCTCTCTCTTG+TGG | + | Chr6:31012426-31012445 | None:intergenic | 30.0% | |
| TTGAATCCATTGATGAAAAC+GGG | - | Chr6:31012247-31012266 | MsG0680031974.01.T01:CDS | 30.0% | |
| TTTAACAACCATTCGTTTAG+AGG | + | Chr6:31011854-31011873 | None:intergenic | 30.0% | |
| ! | ATAATTCTTTTCCGCACAAT+TGG | - | Chr6:31012829-31012848 | MsG0680031974.01.T01:CDS | 30.0% |
| ! | GAATTTATATTGGTTGCTGA+AGG | - | Chr6:31012505-31012524 | MsG0680031974.01.T01:CDS | 30.0% |
| !! | AAGAGAGAGATTTTGATAAC+AGG | - | Chr6:31012427-31012446 | MsG0680031974.01.T01:CDS | 30.0% |
| !! | TTGTAAGTTGTTTTATCCGA+AGG | + | Chr6:31011922-31011941 | None:intergenic | 30.0% |
| AGCCTTTATGCTCCTTTAAA+TGG | - | Chr6:31012142-31012161 | MsG0680031974.01.T01:CDS | 35.0% | |
| ATCGTGGATGCTTATAGAAT+CGG | - | Chr6:31012745-31012764 | MsG0680031974.01.T01:CDS | 35.0% | |
| ATGTCTTTCCTCTAAACGAA+TGG | - | Chr6:31011843-31011862 | MsG0680031974.01.T01:CDS | 35.0% | |
| GGTCAAACACTCTTAACTTT+GGG | + | Chr6:31012303-31012322 | None:intergenic | 35.0% | |
| TAAAGGCTTTCACCATTTAC+AGG | + | Chr6:31012130-31012149 | None:intergenic | 35.0% | |
| TAAGTTTATCTGTCAGCCTT+CGG | - | Chr6:31011903-31011922 | MsG0680031974.01.T01:CDS | 35.0% | |
| TCAATGCAATACACAATTGG+AGG | + | Chr6:31012810-31012829 | None:intergenic | 35.0% | |
| TGCATTTATCCTTAGGAGTT+TGG | - | Chr6:31011789-31011808 | MsG0680031974.01.T01:CDS | 35.0% | |
| TTAGTTTCTGCGATAACCAA+TGG | - | Chr6:31011752-31011771 | MsG0680031974.01.T01:CDS | 35.0% | |
| ! | CTTAGGAGTTTGGATTTTAG+AGG | - | Chr6:31011799-31011818 | MsG0680031974.01.T01:CDS | 35.0% |
| ! | GACCATTTAAAGGAGCATAA+AGG | + | Chr6:31012147-31012166 | None:intergenic | 35.0% |
| ! | TCTTTAGCAAGATCTCGTTT+TGG | - | Chr6:31012221-31012240 | MsG0680031974.01.T01:CDS | 35.0% |
| !! | AGGATTCTTTGCTTTTCTCA+AGG | - | Chr6:31012447-31012466 | MsG0680031974.01.T01:CDS | 35.0% |
| !! | CTCTAAAATCCAAACTCCTA+AGG | + | Chr6:31011801-31011820 | None:intergenic | 35.0% |
| !! | GGATTTTAGAGGAGAAGTTA+AGG | - | Chr6:31011810-31011829 | MsG0680031974.01.T01:CDS | 35.0% |
| !! | TTGTGTTGTAAGTTTGCTAG+TGG | + | Chr6:31012909-31012928 | None:intergenic | 35.0% |
| AAAACCGACGAATGTGATGA+TGG | + | Chr6:31012385-31012404 | None:intergenic | 40.0% | |
| CATCGTACTAGACTTCGAAA+AGG | + | Chr6:31011719-31011738 | None:intergenic | 40.0% | |
| CGATAACCAATGGCTTTCTA+GGG | - | Chr6:31011762-31011781 | MsG0680031974.01.T01:CDS | 40.0% | |
| CGGTCAAACACTCTTAACTT+TGG | + | Chr6:31012304-31012323 | None:intergenic | 40.0% | |
| CGTTTAGAGGAAAGACATCA+TGG | + | Chr6:31011841-31011860 | None:intergenic | 40.0% | |
| GAATCGGAGAATTTCCATTC+TGG | - | Chr6:31012761-31012780 | MsG0680031974.01.T01:CDS | 40.0% | |
| GCTCTCATGTTCATTGTTTC+TGG | - | Chr6:31011965-31011984 | MsG0680031974.01.T01:intron | 40.0% | |
| GGGTTGTTGCATTTATCCTT+AGG | - | Chr6:31011782-31011801 | MsG0680031974.01.T01:CDS | 40.0% | |
| TATCCGTGTCTTATGCGATT+GGG | - | Chr6:31012002-31012021 | MsG0680031974.01.T01:intron | 40.0% | |
| TATTTCCGCAGTAGACTTGT+TGG | - | Chr6:31012616-31012635 | MsG0680031974.01.T01:CDS | 40.0% | |
| TGAACCATCATCACATTCGT+CGG | - | Chr6:31012378-31012397 | MsG0680031974.01.T01:CDS | 40.0% | |
| TGTGAAGTCGATCCTGTAAA+TGG | - | Chr6:31012115-31012134 | MsG0680031974.01.T01:CDS | 40.0% | |
| TTATCCGTGTCTTATGCGAT+TGG | - | Chr6:31012001-31012020 | MsG0680031974.01.T01:intron | 40.0% | |
| ! | AACTCTTTCATGGACTAACG+TGG | - | Chr6:31011873-31011892 | MsG0680031974.01.T01:CDS | 40.0% |
| ! | AACTTACAAGAGCATGGTTG+TGG | - | Chr6:31011933-31011952 | MsG0680031974.01.T01:intron | 40.0% |
| ! | AAGGTACTCACACTATCTTC+TGG | - | Chr6:31012466-31012485 | MsG0680031974.01.T01:CDS | 40.0% |
| ! | AATATCTAAAGCTGCCTCGA+GGG | + | Chr6:31012041-31012060 | None:intergenic | 40.0% |
| ! | GGGTCAAGAGAAAACACTTT+TGG | - | Chr6:31012267-31012286 | MsG0680031974.01.T01:CDS | 40.0% |
| ! | TCTTGACCCGTTTTCATCAA+TGG | + | Chr6:31012256-31012275 | None:intergenic | 40.0% |
| ! | TTAGCAAGATCTCGTTTTGG+TGG | - | Chr6:31012224-31012243 | MsG0680031974.01.T01:CDS | 40.0% |
| !! | AAAGTGTTGAAGCGATATGC+AGG | - | Chr6:31012999-31013018 | MsG0680031974.01.T01:CDS | 40.0% |
| !! | AAGAGTCCAGCTTTGGAAAA+AGG | + | Chr6:31012205-31012224 | None:intergenic | 40.0% |
| !! | AAGTGTTGAAGCGATATGCA+GGG | - | Chr6:31013000-31013019 | MsG0680031974.01.T01:CDS | 40.0% |
| !! | AGAGTCCAGCTTTGGAAAAA+GGG | + | Chr6:31012204-31012223 | None:intergenic | 40.0% |
| !! | GAGATTAAAGGCTAGTTCTG+AGG | - | Chr6:31012962-31012981 | MsG0680031974.01.T01:CDS | 40.0% |
| !!! | CATCACATTCGTCGGTTTTA+TGG | - | Chr6:31012386-31012405 | MsG0680031974.01.T01:CDS | 40.0% |
| CAACAACCCTAGAAAGCCAT+TGG | + | Chr6:31011771-31011790 | None:intergenic | 45.0% | |
| CAAGTCTGGTGAAACCAGAA+TGG | + | Chr6:31012778-31012797 | None:intergenic | 45.0% | |
| CAAGTTTCAACCCCAACTCA+TGG | - | Chr6:31012926-31012945 | MsG0680031974.01.T01:CDS | 45.0% | |
| CGCGCAAGATGAGAGATTAA+AGG | - | Chr6:31012950-31012969 | MsG0680031974.01.T01:CDS | 45.0% | |
| CTTGCTAAAGAGTCCAGCTT+TGG | + | Chr6:31012212-31012231 | None:intergenic | 45.0% | |
| GCGATAACCAATGGCTTTCT+AGG | - | Chr6:31011761-31011780 | MsG0680031974.01.T01:CDS | 45.0% | |
| GCTAGATTGAATCACTCTGG+TGG | - | Chr6:31012703-31012722 | MsG0680031974.01.T01:CDS | 45.0% | |
| TAGACTTCGAAAAGGTCTCG+AGG | + | Chr6:31011711-31011730 | None:intergenic | 45.0% | |
| TCACTCCAACAAGTCTACTG+CGG | + | Chr6:31012624-31012643 | None:intergenic | 45.0% | |
| TCGGCTAGATTGAATCACTC+TGG | - | Chr6:31012700-31012719 | MsG0680031974.01.T01:CDS | 45.0% | |
| ! | CGTTTGCTAGTCCAATTGTG+CGG | + | Chr6:31012843-31012862 | None:intergenic | 45.0% |
| ! | GAATATCTAAAGCTGCCTCG+AGG | + | Chr6:31012042-31012061 | None:intergenic | 45.0% |
| ! | GTAACCCCTTTTTCCAAAGC+TGG | - | Chr6:31012196-31012215 | MsG0680031974.01.T01:CDS | 45.0% |
| ! | TCATGGACTAACGTGGTTGT+CGG | - | Chr6:31011880-31011899 | MsG0680031974.01.T01:CDS | 45.0% |
| !! | CATTCTGGTTTCACCAGACT+TGG | - | Chr6:31012776-31012795 | MsG0680031974.01.T01:CDS | 45.0% |
| !! | GAGTCCAGCTTTGGAAAAAG+GGG | + | Chr6:31012203-31012222 | None:intergenic | 45.0% |
| ATCTTGCGCGACCATGAGTT+GGG | + | Chr6:31012940-31012959 | None:intergenic | 50.0% | |
| GACTAGCAAACGCGCATCAT+TGG | - | Chr6:31012851-31012870 | MsG0680031974.01.T01:CDS | 50.0% | |
| GCTCCCAATCGCATAAGACA+CGG | + | Chr6:31012008-31012027 | None:intergenic | 50.0% | |
| TAGCAAACGCGCATCATTGG+CGG | - | Chr6:31012854-31012873 | MsG0680031974.01.T01:CDS | 50.0% | |
| !! | AAGGCTAGTTCTGAGGCTCT+TGG | - | Chr6:31012969-31012988 | MsG0680031974.01.T01:CDS | 50.0% |
| !! | GTTCTGAGGCTCTTGGACTA+TGG | - | Chr6:31012976-31012995 | MsG0680031974.01.T01:CDS | 50.0% |
| CATCTTGCGCGACCATGAGT+TGG | + | Chr6:31012941-31012960 | None:intergenic | 55.0% | |
| TCTTGCGCGACCATGAGTTG+GGG | + | Chr6:31012939-31012958 | None:intergenic | 55.0% | |
| !! | GGAGGCTTGTTGTCCAAGTC+TGG | + | Chr6:31012792-31012811 | None:intergenic | 55.0% |
| GAGCAGTGAACTGTCCCTCG+AGG | - | Chr6:31012024-31012043 | MsG0680031974.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 31011692 | 31013046 | 31011692 | ID=MsG0680031974.01;Name=MsG0680031974.01 |
| Chr6 | mRNA | 31011692 | 31013046 | 31011692 | ID=MsG0680031974.01.T01;Parent=MsG0680031974.01;Name=MsG0680031974.01.T01;_AED=0.42;_eAED=0.45;_QI=0|0|0|1|1|1|2|0|428 |
| Chr6 | exon | 31012014 | 31013046 | 31012014 | ID=MsG0680031974.01.T01:exon:15032;Parent=MsG0680031974.01.T01 |
| Chr6 | exon | 31011692 | 31011945 | 31011692 | ID=MsG0680031974.01.T01:exon:15031;Parent=MsG0680031974.01.T01 |
| Chr6 | CDS | 31012014 | 31013046 | 31012014 | ID=MsG0680031974.01.T01:cds;Parent=MsG0680031974.01.T01 |
| Chr6 | CDS | 31011692 | 31011945 | 31011692 | ID=MsG0680031974.01.T01:cds;Parent=MsG0680031974.01.T01 |
| Gene Sequence |
| Protein sequence |
