AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029441.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029441.01.T01 MTR_5g086260 78.531 177 38 0 1 177 139 315 5.37e-98 286
MsG0580029441.01.T01 MTR_5g086340 65.714 175 59 1 1 175 264 437 2.10e-74 230
MsG0580029441.01.T01 MTR_5g087580 61.143 175 67 1 3 177 48 221 9.75e-73 218
MsG0580029441.01.T01 MTR_8g042830 55.676 185 72 2 1 176 236 419 4.00e-64 203
MsG0580029441.01.T01 MTR_5g016570 46.857 175 91 2 2 175 358 531 2.44e-39 140
MsG0580029441.01.T01 MTR_7g011980 35.795 176 110 3 2 175 172 346 2.67e-33 121
MsG0580029441.01.T01 MTR_6g010850 37.572 173 105 3 2 172 179 350 4.66e-33 121
MsG0580029441.01.T01 MTR_6g011050 39.286 168 99 3 2 167 239 405 3.92e-32 119
MsG0580029441.01.T01 MTR_6g010920 37.278 169 102 4 2 167 238 405 1.57e-31 117
MsG0580029441.01.T01 MTR_6g011060 37.079 178 105 4 2 175 249 423 2.13e-31 117
MsG0580029441.01.T01 MTR_7g060310 38.857 175 102 2 1 171 315 488 3.36e-31 117
MsG0580029441.01.T01 MTR_6g009590 36.158 177 110 3 2 176 241 416 3.78e-30 114
MsG0580029441.01.T01 MTR_7g059480 40.571 175 95 4 2 170 602 773 9.11e-30 114
MsG0580029441.01.T01 MTR_0024s0080 36.932 176 108 3 2 175 236 410 1.98e-29 112
MsG0580029441.01.T01 MTR_7g059525 39.884 173 99 2 2 170 112 283 2.85e-29 109
MsG0580029441.01.T01 MTR_7g060840 38.953 172 100 2 3 170 262 432 4.07e-29 111
MsG0580029441.01.T01 MTR_6g009580 35.838 173 108 3 2 172 65 236 6.15e-29 107
MsG0580029441.01.T01 MTR_2g035640 33.333 165 108 2 2 165 180 343 1.08e-28 109
MsG0580029441.01.T01 MTR_6g010810 36.158 177 109 4 2 175 237 412 3.03e-28 108
MsG0580029441.01.T01 MTR_6g009610 35.795 176 110 3 2 175 241 415 3.07e-28 108
MsG0580029441.01.T01 MTR_6g010890 34.483 174 105 4 2 167 250 422 5.22e-28 108
MsG0580029441.01.T01 MTR_7g060080 35.260 173 107 2 2 170 20 191 9.27e-28 103
MsG0580029441.01.T01 MTR_7g059540 38.150 173 102 2 2 170 295 466 1.62e-27 107
MsG0580029441.01.T01 MTR_7g059540 38.150 173 102 2 2 170 304 475 1.62e-27 107
MsG0580029441.01.T01 MTR_7g060260 38.506 174 102 2 2 171 255 427 1.80e-27 107
MsG0580029441.01.T01 MTR_7g060330 38.857 175 101 2 1 171 316 488 8.27e-27 105
MsG0580029441.01.T01 MTR_3g107590 34.463 177 113 3 2 176 251 426 1.01e-26 105
MsG0580029441.01.T01 MTR_6g009170 37.634 186 105 6 2 178 245 428 1.52e-26 104
MsG0580029441.01.T01 MTR_8g042170 33.511 188 107 4 2 171 271 458 5.60e-26 103
MsG0580029441.01.T01 MTR_8g042400 33.511 188 107 4 2 171 158 345 7.07e-25 99.0
MsG0580029441.01.T01 MTR_8g037923 34.759 187 96 5 2 166 284 466 2.19e-24 99.0
MsG0580029441.01.T01 MTR_8g037920 34.659 176 91 4 11 166 234 405 3.34e-24 97.8
MsG0580029441.01.T01 MTR_7g060020 34.783 184 100 4 2 178 265 435 1.13e-23 96.7
MsG0580029441.01.T01 MTR_7g059520 35.632 174 97 4 2 170 9 172 1.18e-23 92.0
MsG0580029441.01.T01 MTR_7g060320 39.655 174 100 2 1 170 292 464 1.80e-22 93.6
MsG0580029441.01.T01 MTR_0182s0060 35.838 173 105 3 2 170 346 516 8.65e-22 91.7
MsG0580029441.01.T01 MTR_3g079390 30.409 171 116 3 2 170 280 449 1.82e-21 90.5
MsG0580029441.01.T01 MTR_2g036320 35.057 174 103 6 2 167 290 461 2.03e-21 90.9
MsG0580029441.01.T01 MTR_7g060115 34.254 181 105 3 2 178 285 455 2.12e-21 90.5
MsG0580029441.01.T01 MTR_8g042070 32.292 192 104 5 2 171 77 264 3.74e-20 85.1
MsG0580029441.01.T01 MTR_8g005225 26.804 194 120 3 3 176 271 462 3.92e-20 87.0
MsG0580029441.01.T01 MTR_6g009600 39.623 106 62 2 2 106 27 131 5.76e-20 82.0
MsG0580029441.01.T01 MTR_7g060160 32.044 181 109 4 2 178 237 407 1.64e-19 84.7
MsG0580029441.01.T01 MTR_7g446270 34.706 170 106 2 1 166 312 480 3.84e-19 84.0
MsG0580029441.01.T01 MTR_7g059510 33.143 175 102 4 1 170 253 417 1.16e-18 82.4
MsG0580029441.01.T01 MTR_7g060370 36.207 174 103 4 2 170 300 470 3.44e-18 81.3
MsG0580029441.01.T01 MTR_8g005265 28.125 192 116 3 2 173 266 455 5.73e-18 80.9
MsG0580029441.01.T01 MTR_2g041180 32.584 178 102 8 2 167 274 445 3.67e-17 78.6
MsG0580029441.01.T01 MTR_2g036310 32.704 159 97 6 20 170 2 158 1.06e-16 73.6
MsG0580029441.01.T01 MTR_8g005325 29.348 184 104 4 2 163 284 463 1.63e-16 76.6
MsG0580029441.01.T01 MTR_7g060220 34.104 173 108 3 2 170 266 436 1.09e-15 74.3
MsG0580029441.01.T01 MTR_8g005235 27.604 192 113 6 2 171 254 441 6.34e-15 72.0
MsG0580029441.01.T01 MTR_7g059810 34.104 173 108 3 2 170 274 444 2.78e-14 70.1
MsG0580029441.01.T01 MTR_0209s0060 29.609 179 98 6 2 161 257 426 6.02e-14 69.3
MsG0580029441.01.T01 MTR_0209s0030 29.609 179 98 6 2 161 257 426 6.02e-14 69.3
MsG0580029441.01.T01 MTR_8g005295 27.072 181 106 4 2 158 319 497 2.19e-13 67.8
MsG0580029441.01.T01 MTR_4g009590 28.729 181 112 7 6 176 386 559 6.58e-13 66.2
MsG0580029441.01.T01 MTR_3g107600 26.923 156 86 4 2 156 443 571 1.02e-11 62.8
MsG0580029441.01.T01 MTR_4g009620 27.419 186 116 8 2 176 380 557 2.26e-11 62.0
MsG0580029441.01.T01 MTR_8g005285 25.414 181 109 4 2 158 305 483 8.78e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029441.01.T01 AT3G50120 42.857 175 98 2 2 175 355 528 5.76e-41 144
MsG0580029441.01.T01 AT3G50160 39.429 175 104 2 2 175 325 498 2.92e-38 137
MsG0580029441.01.T01 AT3G50140 39.429 175 104 2 2 175 363 536 6.22e-38 136
MsG0580029441.01.T01 AT3G50170 39.429 175 104 2 2 175 363 536 1.48e-37 135
MsG0580029441.01.T01 AT3G50150 38.857 175 105 2 2 175 333 506 1.51e-37 135
MsG0580029441.01.T01 AT4G31980 37.647 170 103 2 2 170 510 677 4.50e-32 121
MsG0580029441.01.T01 AT3G50130 38.506 174 105 2 2 174 388 560 9.68e-31 117
MsG0580029441.01.T01 AT3G50180 34.270 178 111 2 2 174 408 584 6.52e-28 109
MsG0580029441.01.T01 AT3G50190 31.461 178 117 3 2 175 314 490 1.95e-26 104
MsG0580029441.01.T01 AT5G11290 32.353 170 111 3 2 170 184 350 2.77e-26 102
MsG0580029441.01.T01 AT5G11290 32.353 170 111 3 2 170 253 419 5.86e-26 102
MsG0580029441.01.T01 AT3G60470 35.795 176 110 3 2 176 367 540 2.13e-23 96.3
MsG0580029441.01.T01 AT5G22540 34.028 144 92 3 7 148 266 408 7.67e-22 91.7
MsG0580029441.01.T01 AT2G44930 31.148 183 114 4 2 178 327 503 3.64e-21 90.1
MsG0580029441.01.T01 AT3G47250 30.114 176 119 4 3 175 303 477 8.31e-20 86.3
MsG0580029441.01.T01 AT3G47250 30.114 176 119 4 3 175 303 477 8.31e-20 86.3
MsG0580029441.01.T01 AT3G47250 30.114 176 119 4 3 175 303 477 8.31e-20 86.3
MsG0580029441.01.T01 AT2G36430 33.898 177 114 3 2 176 273 448 1.37e-19 85.5
MsG0580029441.01.T01 AT3G44710 29.545 176 122 2 2 175 326 501 1.57e-19 85.5
MsG0580029441.01.T01 AT5G22560 30.178 169 115 3 2 168 340 507 4.13e-19 84.3
MsG0580029441.01.T01 AT2G28580 31.638 177 109 5 2 172 330 500 5.75e-19 84.0
MsG0580029441.01.T01 AT2G28580 31.410 156 97 3 21 172 368 517 5.96e-19 83.6
MsG0580029441.01.T01 AT2G28580 31.410 156 97 3 21 172 365 514 6.12e-19 83.6
MsG0580029441.01.T01 AT2G28580 31.638 177 109 5 2 172 279 449 6.25e-19 83.6
MsG0580029441.01.T01 AT2G28580 31.410 156 97 3 21 172 382 531 6.77e-19 83.6
MsG0580029441.01.T01 AT5G22550 28.409 176 123 3 2 175 313 487 5.55e-18 80.9
MsG0580029441.01.T01 AT5G22550 28.409 176 123 3 2 175 264 438 6.09e-18 80.5
MsG0580029441.01.T01 AT3G47200 26.163 172 122 4 3 170 285 455 6.40e-16 75.1
MsG0580029441.01.T01 AT3G47200 26.163 172 122 4 3 170 285 455 6.40e-16 75.1
MsG0580029441.01.T01 AT1G67150 27.647 170 122 1 3 172 193 361 4.50e-13 66.6
MsG0580029441.01.T01 AT1G67150 27.647 170 122 1 3 172 236 404 4.66e-13 66.6
MsG0580029441.01.T01 AT1G67150 27.647 170 122 1 3 172 236 404 4.66e-13 66.6
MsG0580029441.01.T01 AT1G67150 27.647 170 122 1 3 172 240 408 4.74e-13 66.6
MsG0580029441.01.T01 AT3G02645 28.000 150 105 2 2 149 350 498 3.12e-11 61.6

Find 38 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAACGAGTTGAACAATT+TGG 0.245536 5:-97017715 None:intergenic
CGTGTTGTCTACTACTATTA+AGG 0.283367 5:-97017517 None:intergenic
AAAAGATGTTACCTTGTTAT+CGG 0.302978 5:-97017592 None:intergenic
CCTTCAATCGAGGTGTTCTT+AGG 0.321521 5:+97017487 MsG0580029441.01.T01:CDS
CGTTCTCAAGATAGTTGATT+AGG 0.331878 5:-97017680 None:intergenic
GAATAATAGTAAGAGCAAAA+AGG 0.409383 5:-97017893 None:intergenic
GCTCCAAGGCCATGAGGTTA+AGG 0.418526 5:-97017551 None:intergenic
ATTCCTTAACCTCATGGCCT+TGG 0.421735 5:+97017548 MsG0580029441.01.T01:CDS
ATGGATTGCAATGGAGAACT+TGG 0.448261 5:+97017750 MsG0580029441.01.T01:CDS
ACACTTACTTTAAGAATCCA+TGG 0.451026 5:+97017841 MsG0580029441.01.T01:CDS
GTGTTGTCTACTACTATTAA+GGG 0.456703 5:-97017516 None:intergenic
CCTAAGAACACCTCGATTGA+AGG 0.471335 5:-97017487 None:intergenic
AAGTTCTCCATTGCAATCCA+TGG 0.472099 5:-97017748 None:intergenic
GCAATGGAGCTTAAAGAGGC+TGG 0.505520 5:+97017420 None:intergenic
AGCCAAATTGTTCAACTCGT+TGG 0.520680 5:+97017713 MsG0580029441.01.T01:CDS
AGTTCTCCATTGCAATCCAT+GGG 0.524419 5:-97017747 None:intergenic
CACTTACTTTAAGAATCCAT+GGG 0.526297 5:+97017842 MsG0580029441.01.T01:CDS
GTAGGAAACCGTGGAAAAGA+TGG 0.537234 5:+97017802 MsG0580029441.01.T01:CDS
ATACATATTCCTTAACCTCA+TGG 0.539977 5:+97017542 MsG0580029441.01.T01:CDS
ATCTGCAATGGAGCTTAAAG+AGG 0.545147 5:+97017416 None:intergenic
ATTCAAACTATATACACAGT+TGG 0.561149 5:+97017912 MsG0580029441.01.T01:CDS
CAATGGAGAACTTGGAGAAG+TGG 0.569190 5:+97017758 MsG0580029441.01.T01:CDS
CGTAGTTGAAGCCGATAACA+AGG 0.577409 5:+97017581 MsG0580029441.01.T01:CDS
AGAGTGTGATTTCTTACTGT+AGG 0.595533 5:+97017784 MsG0580029441.01.T01:CDS
ACAAGAGATACAATAGCCCA+TGG 0.623258 5:-97017858 None:intergenic
TTTCTTACTGTAGGAAACCG+TGG 0.625114 5:+97017793 MsG0580029441.01.T01:CDS
AGTTGATTAGGATATGACTT+CGG 0.631023 5:-97017668 None:intergenic
TGTCTACTACTATTAAGGGC+GGG 0.632807 5:-97017512 None:intergenic
AACTACGTGGAGACGCTCCA+AGG 0.645173 5:-97017565 None:intergenic
AGAGATGTTTCCTTCAATCG+AGG 0.652766 5:+97017477 MsG0580029441.01.T01:CDS
TGTTATCGGCTTCAACTACG+TGG 0.657789 5:-97017578 None:intergenic
TTCTCCATTGCAATCCATGG+GGG 0.668558 5:-97017745 None:intergenic
GCTTCAAGAAAAGCGAAACG+AGG 0.673060 5:+97017448 MsG0580029441.01.T01:CDS
TTGTCTACTACTATTAAGGG+CGG 0.675393 5:-97017513 None:intergenic
CTTAATAGTAGTAGACAACA+CGG 0.685653 5:+97017518 MsG0580029441.01.T01:CDS
GTTCTCCATTGCAATCCATG+GGG 0.702774 5:-97017746 None:intergenic
GGAGACGCTCCAAGGCCATG+AGG 0.715446 5:-97017557 None:intergenic
GATTGAAGGAAACATCTCTG+AGG 0.729285 5:-97017473 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTCAAACTATATACACAGT+TGG + Chr5:97017912-97017931 MsG0580029441.01.T01:CDS 25.0%
! GAATAATAGTAAGAGCAAAA+AGG - Chr5:97017896-97017915 None:intergenic 25.0%
!! AAAAGATGTTACCTTGTTAT+CGG - Chr5:97017595-97017614 None:intergenic 25.0%
!! AACATCTTTTGTATGCTTTA+TGG + Chr5:97017605-97017624 MsG0580029441.01.T01:CDS 25.0%
!! AGTTGGTCAATTTTATCAAA+AGG + Chr5:97017929-97017948 MsG0580029441.01.T01:CDS 25.0%
AGTTGATTAGGATATGACTT+CGG - Chr5:97017671-97017690 None:intergenic 30.0%
ATACATATTCCTTAACCTCA+TGG + Chr5:97017542-97017561 MsG0580029441.01.T01:CDS 30.0%
CTTAATAGTAGTAGACAACA+CGG + Chr5:97017518-97017537 MsG0580029441.01.T01:CDS 30.0%
GTGTTGTCTACTACTATTAA+GGG - Chr5:97017519-97017538 None:intergenic 30.0%
! ACACTTACTTTAAGAATCCA+TGG + Chr5:97017841-97017860 MsG0580029441.01.T01:CDS 30.0%
! CACTTACTTTAAGAATCCAT+GGG + Chr5:97017842-97017861 MsG0580029441.01.T01:CDS 30.0%
! GGTCAATTTTATCAAAAGGA+GGG + Chr5:97017933-97017952 MsG0580029441.01.T01:CDS 30.0%
! TGGTCAATTTTATCAAAAGG+AGG + Chr5:97017932-97017951 MsG0580029441.01.T01:CDS 30.0%
AGAGTGTGATTTCTTACTGT+AGG + Chr5:97017784-97017803 MsG0580029441.01.T01:CDS 35.0%
CGTGTTGTCTACTACTATTA+AGG - Chr5:97017520-97017539 None:intergenic 35.0%
CGTTCTCAAGATAGTTGATT+AGG - Chr5:97017683-97017702 None:intergenic 35.0%
TTGTCTACTACTATTAAGGG+CGG - Chr5:97017516-97017535 None:intergenic 35.0%
AAGAGCAAAAAGGAAGACAG+CGG - Chr5:97017886-97017905 None:intergenic 40.0%
AAGTTCTCCATTGCAATCCA+TGG - Chr5:97017751-97017770 None:intergenic 40.0%
ACAAGAGATACAATAGCCCA+TGG - Chr5:97017861-97017880 None:intergenic 40.0%
AGAGATGTTTCCTTCAATCG+AGG + Chr5:97017477-97017496 MsG0580029441.01.T01:CDS 40.0%
AGCCAAATTGTTCAACTCGT+TGG + Chr5:97017713-97017732 MsG0580029441.01.T01:CDS 40.0%
AGTTCTCCATTGCAATCCAT+GGG - Chr5:97017750-97017769 None:intergenic 40.0%
GATTGAAGGAAACATCTCTG+AGG - Chr5:97017476-97017495 None:intergenic 40.0%
TGTCTACTACTATTAAGGGC+GGG - Chr5:97017515-97017534 None:intergenic 40.0%
TTTCTTACTGTAGGAAACCG+TGG + Chr5:97017793-97017812 MsG0580029441.01.T01:CDS 40.0%
! ATGGATTGCAATGGAGAACT+TGG + Chr5:97017750-97017769 MsG0580029441.01.T01:CDS 40.0%
! TGCCAACGAGTTGAACAATT+TGG - Chr5:97017718-97017737 None:intergenic 40.0%
ATTCCTTAACCTCATGGCCT+TGG + Chr5:97017548-97017567 MsG0580029441.01.T01:CDS 45.0%
CAATGGAGAACTTGGAGAAG+TGG + Chr5:97017758-97017777 MsG0580029441.01.T01:CDS 45.0%
CCTAAGAACACCTCGATTGA+AGG - Chr5:97017490-97017509 None:intergenic 45.0%
CGTAGTTGAAGCCGATAACA+AGG + Chr5:97017581-97017600 MsG0580029441.01.T01:CDS 45.0%
GCTTCAAGAAAAGCGAAACG+AGG + Chr5:97017448-97017467 MsG0580029441.01.T01:CDS 45.0%
GTAGGAAACCGTGGAAAAGA+TGG + Chr5:97017802-97017821 MsG0580029441.01.T01:CDS 45.0%
GTTCTCCATTGCAATCCATG+GGG - Chr5:97017749-97017768 None:intergenic 45.0%
TTCTCCATTGCAATCCATGG+GGG - Chr5:97017748-97017767 None:intergenic 45.0%
! CCTTCAATCGAGGTGTTCTT+AGG + Chr5:97017487-97017506 MsG0580029441.01.T01:CDS 45.0%
!! TGTTATCGGCTTCAACTACG+TGG - Chr5:97017581-97017600 None:intergenic 45.0%
! CTTGCACGCCATCTTTTCCA+CGG - Chr5:97017813-97017832 None:intergenic 50.0%
AACTACGTGGAGACGCTCCA+AGG - Chr5:97017568-97017587 None:intergenic 55.0%
GCTCCAAGGCCATGAGGTTA+AGG - Chr5:97017554-97017573 None:intergenic 55.0%
GTTGGCAAAAGAAGCCCCCA+TGG + Chr5:97017731-97017750 MsG0580029441.01.T01:CDS 55.0%
! GAAGCCCCCATGGATTGCAA+TGG + Chr5:97017741-97017760 MsG0580029441.01.T01:CDS 55.0%
GGAGACGCTCCAAGGCCATG+AGG - Chr5:97017560-97017579 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 97017423 97017959 97017423 ID=MsG0580029441.01;Name=MsG0580029441.01
Chr5 mRNA 97017423 97017959 97017423 ID=MsG0580029441.01.T01;Parent=MsG0580029441.01;Name=MsG0580029441.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|178
Chr5 exon 97017423 97017959 97017423 ID=MsG0580029441.01.T01:exon:24802;Parent=MsG0580029441.01.T01
Chr5 CDS 97017423 97017959 97017423 ID=MsG0580029441.01.T01:cds;Parent=MsG0580029441.01.T01
Gene Sequence

>MsG0580029441.01.T01

ATGGAGCTTAAAGAGGCTGGAATCAGCTTCAAGAAAAGCGAAACGAGGAGCCTCAGAGATGTTTCCTTCAATCGAGGTGTTCTTAGGCTCCCGCCCTTAATAGTAGTAGACAACACGGAATACATATTCCTTAACCTCATGGCCTTGGAGCGTCTCCACGTAGTTGAAGCCGATAACAAGGTAACATCTTTTGTATGCTTTATGGACACTATCATCGACAGTGCTAAAGATGTTGAAATTCTCATCCGAAGTCATATCCTAATCAACTATCTTGAGAACGATGAAGCTGTAGCCAAATTGTTCAACTCGTTGGCAAAAGAAGCCCCCATGGATTGCAATGGAGAACTTGGAGAAGTGGCGAAGAGTGTGATTTCTTACTGTAGGAAACCGTGGAAAAGATGGCGTGCAAGTCTCATTCACACTTACTTTAAGAATCCATGGGCTATTGTATCTCTTGTCGCCGCTGTCTTCCTTTTTGCTCTTACTATTATTCAAACTATATACACAGTTGGTCAATTTTATCAAAAGGAGGGCTAG

Protein sequence

>MsG0580029441.01.T01

MELKEAGISFKKSETRSLRDVSFNRGVLRLPPLIVVDNTEYIFLNLMALERLHVVEADNKVTSFVCFMDTIIDSAKDVEILIRSHILINYLENDEAVAKLFNSLAKEAPMDCNGELGEVAKSVISYCRKPWKRWRASLIHTYFKNPWAIVSLVAAVFLFALTIIQTIYTVGQFYQKEG*