AlfalfaGEDB | MsG0680032024.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032024.01

KEGG: K05626
GO: GO:0005576
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032024.01.T01	EFJ35139.1	50.829	181	74	6	1	167	117	296	2.52E-23	106
MsG0680032024.01.T01	EFJ35139.1	50	182	60	5	2	167	93	259	2.64E-21	100
MsG0680032024.01.T01	EFJ35139.1	66.372	113	31	3	2	108	192	303	1.10E-19	96.7
MsG0680032024.01.T01	EFJ35139.1	48.649	148	45	4	2	146	229	348	1.77E-14	82
MsG0680032024.01.T01	EFJ35139.1	43.085	188	77	6	10	167	35	222	1.50E-12	77
MsG0680032024.01.T02	EFJ35139.1	50	174	72	6	3	162	124	296	6.01E-21	99.8
MsG0680032024.01.T02	EFJ35139.1	52.601	173	64	6	2	162	140	306	7.99E-19	94
MsG0680032024.01.T02	EFJ35139.1	48.966	145	59	4	2	141	214	348	3.74E-13	78.6
MsG0680032024.01.T02	EFJ35139.1	43.085	188	77	6	5	162	35	222	5.44E-13	77.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032024.01.T01	D8QY50	50.829	181	74	6	1	167	117	296	1.21e-23	106
MsG0680032024.01.T02	D8QY50	50.000	174	72	6	3	162	124	296	2.87e-21	99.8

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004202.01	MsG0680032024.01	0.800035	1.740908e-48	8.023493e-46
MsG0480022833.01	MsG0680032024.01	0.817775	2.768031e-52	2.034589e-49
MsG0680032024.01	MsG0680032026.01	0.816745	4.719015e-52	3.372034e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032024.01.T01	MTR_6g034870	47.205	161	5	4	3	108	117	252	5.24e-13	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 124 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCACCATTTAGTAGTGGTA+AGG	0.552807	6:-32133351	None:intergenic
ACCATTTAGTAGTGGTAAGG+AGG	0.618262	6:-32133348	None:intergenic
ACGTATTGTTTGCTGAAATT+TGG	0.144133	6:-32132689	None:intergenic
ACTTACTTGTAAACTGGTGG+AGG	0.605274	6:-32133268	None:intergenic
ACTTGTAAACTGGTGGAGGG+TGG	0.727436	6:-32132963	None:intergenic
ACTTGTAAACTGGTTGGTGG+GGG	0.563135	6:-32132756	None:intergenic
AGATATAATGTGGTGGAGGT+GGG	0.524530	6:-32133312	None:intergenic
AGGGACTTGTACTTGTACAC+CGG	0.620513	6:-32133125	None:intergenic
AGGGTGGTGAGTACACGGGT+TGG	0.617708	6:-32132947	None:intergenic
AGGTATGAGTAGACGGGTGG+AGG	0.483998	6:-32133048	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTGAAGAGTAGATATAATG+TGG	-	Chr6:32133325-32133344	None:intergenic	30.0%
CAATGCAATAGTAAGAGTAA+TGG	-	Chr6:32132643-32132662	None:intergenic	30.0%
GGTGATTTATACTTGTAAAC+TGG	-	Chr6:32132769-32132788	None:intergenic	30.0%
ATACTTGTAAACTGGTTGGT+GGG	-	Chr6:32132761-32132780	None:intergenic	35.0%
GAAGAGTAGATATAATGTGG+TGG	-	Chr6:32133322-32133341	None:intergenic	35.0%
TATACTTGTAAACTGGTTGG+TGG	-	Chr6:32132762-32132781	None:intergenic	35.0%
TATTTATAAACAGGTGGAGG+TGG	-	Chr6:32132811-32132830	None:intergenic	35.0%
TATTTGTATGGCTTAGATGG+TGG	-	Chr6:32132731-32132750	None:intergenic	35.0%
TGCAATAGTAAGAGTAATGG+AGG	-	Chr6:32132640-32132659	None:intergenic	35.0%
ACTTACTTGTAAACTGGTGG+AGG	-	Chr6:32133271-32133290	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	32132621	32133365	32132621	ID=MsG0680032024.01;Name=MsG0680032024.01
Chr6	mRNA	32132710	32133365	32132710	ID=MsG0680032024.01.T01;Parent=MsG0680032024.01;Name=MsG0680032024.01.T01;_AED=0.50;_eAED=0.61;_QI=0\|0\|0\|1\|0\|0\|2\|0\|206
Chr6	exon	32132710	32132734	32132710	ID=MsG0680032024.01.T01:exon:15828;Parent=MsG0680032024.01.T01
Chr6	exon	32132770	32133365	32132770	ID=MsG0680032024.01.T01:exon:15829;Parent=MsG0680032024.01.T01
Chr6	CDS	32132710	32132734	32132710	ID=MsG0680032024.01.T01:cds;Parent=MsG0680032024.01.T01
Chr6	CDS	32132770	32133365	32132770	ID=MsG0680032024.01.T01:cds;Parent=MsG0680032024.01.T01
Chr6	mRNA	32132760	32133365	32132760	ID=MsG0680032024.01.T02;Parent=MsG0680032024.01;Name=MsG0680032024.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|201
Chr6	exon	32132760	32133365	32132760	ID=MsG0680032024.01.T02:exon:15830;Parent=MsG0680032024.01.T02
Chr6	CDS	32132760	32133365	32132760	ID=MsG0680032024.01.T02:cds;Parent=MsG0680032024.01.T02
Chr6	mRNA	32132621	32132782	32132621	ID=MsG0680032024.01.T03;Parent=MsG0680032024.01;Name=MsG0680032024.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|53
Chr6	exon	32132621	32132782	32132621	ID=MsG0680032024.01.T03:exon:15831;Parent=MsG0680032024.01.T03
Chr6	CDS	32132621	32132782	32132621	ID=MsG0680032024.01.T03:cds;Parent=MsG0680032024.01.T03

Gene Sequence

>MsG0680032024.01.T02

CACCAACCAGTTTACAAGTATAAATCACCACCCCCATCTGTCTACTCACCACCTCCACCTGTTTATAAATATAAGTCACCACCTCCACCAGTCTACTCACCACCTCCACCTGTTTACAAGTATAGGTCACCACCTCACACCTGTCTACTCACCACCTCCACCAGTTTACAAGTACAAGTCCCCACCTCCAACCCGTGTACTCACCACCCTCCACCAGTTTACAAGTACAAGTCCCCACCTCCACCCGTCTATTCACCACCACCTCCAGTTTACAAGTACAAGTCCCCACCTCCACCCGTCTACTCATACCTCCACCAGTTTACAAGTACAAGTCTCCTCCTCCACCTGTCTACTCACCACCTCCACCGGTGTACAAGTACAAGTCCCTCCTCCACCCGTCTACTCACCACCTCCACCAGTTTACAATACAAGTCCCCACCCCCCCGTCTACTCACCACCTCCCCCATTTACAAGTACAAGTCACCAGTCTCCTCCCGTCTATTCACCACCTCCACCAGTTTACAAGTAAGTCTCCTCCTCCTCCTACTTACTCCCACCTCCACCACATTATATCTACTCTTCACTCCTCCTCCTTACCACTACTAA

>MsG0680032024.01.T01

GTCTATTCCTCTCCACCACCACCATTTTACAAGTATAAATCACCACCCCCATCTGTCTACTCACCACCTCCACCTGTTTATAAATATAAGTCACCACCTCCACCAGTCTACTCACCACCTCCACCTGTTTACAAGTATAGGTCACCACCTCACACCTGTCTACTCACCACCTCCACCAGTTTACAAGTACAAGTCCCCACCTCCAACCCGTGTACTCACCACCCTCCACCAGTTTACAAGTACAAGTCCCCACCTCCACCCGTCTATTCACCACCACCTCCAGTTTACAAGTACAAGTCCCCACCTCCACCCGTCTACTCATACCTCCACCAGTTTACAAGTACAAGTCTCCTCCTCCACCTGTCTACTCACCACCTCCACCGGTGTACAAGTACAAGTCCCTCCTCCACCCGTCTACTCACCACCTCCACCAGTTTACAATACAAGTCCCCACCCCCCCGTCTACTCACCACCTCCCCCATTTACAAGTACAAGTCACCAGTCTCCTCCCGTCTATTCACCACCTCCACCAGTTTACAAGTAAGTCTCCTCCTCCTCCTACTTACTCCCACCTCCACCACATTATATCTACTCTTCACTCCTCCTCCTTACCACTACTAA

>MsG0680032024.01.T03

ATGAGGTTCCAAATGGCCTCCATTACTCTTACTATTGCATTGACAATAATCTCTCTTAGCTGCCATCCCCAAATTTCAGCAAACAATACGTCTATTCCTCTCCACCACCACCATCTAAGCCATACAAATACGCTTCCCCCACCAACCAGTTTACAAGTATAA

Protein sequence

>MsG0680032024.01.T02

HQPVYKYKSPPPSVYSPPPPVYKYKSPPPPVYSPPPPVYKYRSPPHTCLLTTSTSLQVQVPTSNPCTHHPPPVYKYKSPPPPVYSPPPPVYKYKSPPPPVYSYLHQFTSTSLLLHLSTHHLHRCTSTSPSSTRLLTTSTSLQYKSPPPRLLTTSPIYKYKSPVSSRLFTTSTSLQVSLLLLLLTPTSTTLYLLFTPPPYHY*

>MsG0680032024.01.T01

VYSSPPPPFYKYKSPPPSVYSPPPPVYKYKSPPPPVYSPPPPVYKYRSPPHTCLLTTSTSLQVQVPTSNPCTHHPPPVYKYKSPPPPVYSPPPPVYKYKSPPPPVYSYLHQFTSTSLLLHLSTHHLHRCTSTSPSSTRLLTTSTSLQYKSPPPRLLTTSPIYKYKSPVSSRLFTTSTSLQVSLLLLLLTPTSTTLYLLFTPPPYHY*

>MsG0680032024.01.T03

MRFQMASITLTIALTIISLSCHPQISANNTSIPLHHHHLSHTNTLPPPTSLQV*