Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032349.01.T01 | XP_024630813.1 | 53.247 | 154 | 70 | 2 | 20 | 173 | 90 | 241 | 1.78E-49 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032349.01.T01 | A0A396GZG8 | 47.134 | 157 | 82 | 1 | 17 | 173 | 104 | 259 | 3.95e-40 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680032349.01 | MsG0380013578.01 | PPI |
| MsG0680032349.01 | MsG0780041116.01 | PPI |
| MsG0680032349.01 | MsG0280010449.01 | PPI |
| MsG0680032349.01 | MsG0280009322.01 | PPI |
| MsG0680032349.01 | MsG0780040274.01 | PPI |
| MsG0680032349.01 | MsG0780041116.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032349.01.T01 | MTR_1g088650 | 40.441 | 136 | 80 | 1 | 9 | 144 | 55 | 189 | 2.22e-22 | 89.4 |
| MsG0680032349.01.T01 | MTR_3g010950 | 33.121 | 157 | 74 | 2 | 17 | 173 | 3 | 128 | 3.17e-21 | 85.1 |
| MsG0680032349.01.T01 | MTR_6g089560 | 29.710 | 138 | 96 | 1 | 2 | 139 | 78 | 214 | 5.98e-20 | 84.0 |
| MsG0680032349.01.T01 | MTR_5g083200 | 34.711 | 121 | 78 | 1 | 5 | 125 | 63 | 182 | 1.07e-19 | 82.8 |
| MsG0680032349.01.T01 | MTR_0002s1220 | 34.711 | 121 | 78 | 1 | 5 | 125 | 63 | 182 | 1.07e-19 | 82.8 |
| MsG0680032349.01.T01 | MTR_1g081930 | 38.793 | 116 | 70 | 1 | 17 | 132 | 15 | 129 | 3.02e-19 | 79.7 |
| MsG0680032349.01.T01 | MTR_3g111390 | 33.884 | 121 | 79 | 1 | 5 | 125 | 63 | 182 | 9.65e-19 | 80.1 |
| MsG0680032349.01.T01 | MTR_1g070820 | 33.884 | 121 | 79 | 1 | 5 | 125 | 63 | 182 | 9.65e-19 | 80.1 |
| MsG0680032349.01.T01 | MTR_7g013520 | 30.120 | 166 | 90 | 2 | 8 | 173 | 38 | 177 | 3.19e-18 | 78.6 |
| MsG0680032349.01.T01 | MTR_8g036780 | 39.216 | 102 | 51 | 2 | 15 | 116 | 9 | 99 | 3.82e-18 | 76.3 |
| MsG0680032349.01.T01 | MTR_3g116210 | 37.383 | 107 | 66 | 1 | 18 | 124 | 75 | 180 | 5.00e-18 | 78.2 |
| MsG0680032349.01.T01 | MTR_1g045340 | 37.143 | 105 | 65 | 1 | 21 | 125 | 65 | 168 | 4.52e-17 | 75.5 |
| MsG0680032349.01.T01 | MTR_5g085210 | 32.164 | 171 | 95 | 4 | 2 | 168 | 80 | 233 | 3.35e-16 | 73.9 |
| MsG0680032349.01.T01 | MTR_8g031080 | 30.719 | 153 | 79 | 2 | 18 | 170 | 267 | 392 | 8.22e-16 | 74.7 |
| MsG0680032349.01.T01 | MTR_6g016265 | 36.449 | 107 | 67 | 1 | 21 | 127 | 20 | 125 | 1.24e-15 | 70.1 |
| MsG0680032349.01.T01 | MTR_8g099580 | 36.449 | 107 | 67 | 1 | 18 | 124 | 184 | 289 | 1.96e-15 | 73.2 |
| MsG0680032349.01.T01 | MTR_2g027020 | 32.192 | 146 | 94 | 2 | 28 | 173 | 16 | 156 | 8.94e-15 | 69.3 |
| MsG0680032349.01.T01 | MTR_5g037920 | 34.579 | 107 | 68 | 1 | 17 | 123 | 75 | 179 | 9.42e-15 | 70.1 |
| MsG0680032349.01.T01 | MTR_5g049680 | 39.080 | 87 | 52 | 1 | 21 | 107 | 12 | 97 | 1.07e-14 | 67.0 |
| MsG0680032349.01.T01 | MTR_1g069475 | 32.143 | 112 | 75 | 1 | 9 | 120 | 133 | 243 | 1.09e-14 | 70.1 |
| MsG0680032349.01.T01 | MTR_6g453270 | 34.167 | 120 | 72 | 3 | 8 | 125 | 272 | 386 | 2.06e-14 | 70.5 |
| MsG0680032349.01.T01 | MTR_7g078610 | 57.447 | 47 | 20 | 0 | 13 | 59 | 24 | 70 | 2.10e-14 | 65.9 |
| MsG0680032349.01.T01 | MTR_2g047100 | 33.333 | 114 | 60 | 2 | 27 | 140 | 5 | 102 | 2.53e-14 | 66.2 |
| MsG0680032349.01.T01 | MTR_6g034915 | 28.495 | 186 | 83 | 4 | 18 | 173 | 5 | 170 | 2.63e-14 | 67.8 |
| MsG0680032349.01.T01 | MTR_1g034430 | 35.185 | 108 | 69 | 1 | 21 | 128 | 76 | 182 | 4.13e-14 | 67.4 |
| MsG0680032349.01.T01 | MTR_7g082560 | 42.424 | 99 | 52 | 2 | 79 | 172 | 8 | 106 | 7.54e-14 | 65.1 |
| MsG0680032349.01.T01 | MTR_7g006380 | 35.156 | 128 | 55 | 2 | 27 | 154 | 53 | 152 | 1.11e-13 | 66.2 |
| MsG0680032349.01.T01 | MTR_8g038340 | 43.158 | 95 | 48 | 3 | 6 | 98 | 156 | 246 | 1.22e-13 | 67.4 |
| MsG0680032349.01.T01 | MTR_3g064060 | 54.902 | 51 | 23 | 0 | 9 | 59 | 6 | 56 | 5.31e-13 | 61.2 |
| MsG0680032349.01.T01 | MTR_1g109000 | 40.000 | 80 | 47 | 1 | 21 | 100 | 7 | 85 | 5.89e-13 | 62.0 |
| MsG0680032349.01.T01 | MTR_8g028785 | 33.582 | 134 | 64 | 3 | 15 | 148 | 257 | 365 | 1.52e-12 | 65.1 |
| MsG0680032349.01.T01 | MTR_1g017840 | 32.407 | 108 | 72 | 1 | 17 | 124 | 55 | 161 | 1.57e-12 | 62.8 |
| MsG0680032349.01.T01 | MTR_5g036320 | 40.000 | 75 | 44 | 1 | 21 | 95 | 60 | 133 | 1.97e-12 | 62.4 |
| MsG0680032349.01.T01 | MTR_1g022215 | 39.024 | 82 | 49 | 1 | 28 | 109 | 2 | 82 | 2.42e-12 | 60.5 |
| MsG0680032349.01.T01 | MTR_6g016355 | 39.286 | 84 | 50 | 1 | 19 | 102 | 95 | 177 | 1.43e-11 | 60.5 |
| MsG0680032349.01.T01 | MTR_5g034450 | 38.462 | 78 | 47 | 1 | 27 | 104 | 36 | 112 | 1.72e-11 | 58.9 |
| MsG0680032349.01.T01 | MTR_4g094370 | 61.538 | 39 | 15 | 0 | 23 | 61 | 18 | 56 | 1.86e-11 | 57.8 |
| MsG0680032349.01.T01 | MTR_1g018300 | 31.538 | 130 | 76 | 3 | 1 | 127 | 24 | 143 | 2.08e-11 | 61.2 |
| MsG0680032349.01.T01 | MTR_8g079540 | 40.000 | 75 | 44 | 1 | 13 | 87 | 21 | 94 | 2.28e-11 | 58.9 |
| MsG0680032349.01.T01 | MTR_8g022750 | 34.146 | 123 | 63 | 2 | 16 | 138 | 181 | 285 | 2.84e-11 | 61.2 |
| MsG0680032349.01.T01 | MTR_5g068115 | 54.348 | 46 | 21 | 0 | 15 | 60 | 9 | 54 | 4.54e-11 | 56.6 |
| MsG0680032349.01.T01 | MTR_1g074090 | 35.165 | 91 | 58 | 1 | 17 | 107 | 58 | 147 | 5.92e-11 | 58.5 |
| MsG0680032349.01.T01 | MTR_0015s0120 | 35.227 | 88 | 56 | 1 | 27 | 114 | 280 | 366 | 6.56e-11 | 60.5 |
| MsG0680032349.01.T01 | MTR_4g046027 | 49.123 | 57 | 29 | 0 | 3 | 59 | 90 | 146 | 9.38e-11 | 58.2 |
| MsG0680032349.01.T01 | MTR_4g029360 | 29.600 | 125 | 87 | 1 | 17 | 141 | 104 | 227 | 9.38e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTATTGGATGTGGATA+TGG | 0.221678 | 6:-38963214 | MsG0680032349.01.T01:CDS |
| ATAATGGATCAAAACCTTAT+TGG | 0.281171 | 6:-38963226 | MsG0680032349.01.T01:CDS |
| TCCCATATCCACATCCAATA+AGG | 0.309888 | 6:+38963212 | None:intergenic |
| AGATCATAATATGATGAATA+TGG | 0.312897 | 6:-38963149 | MsG0680032349.01.T01:CDS |
| GTAGGTATTGGAGTTTGTAT+AGG | 0.314694 | 6:-38963283 | MsG0680032349.01.T01:CDS |
| TAAAGATTGTAGACACCTTA+TGG | 0.320244 | 6:-38963047 | MsG0680032349.01.T01:CDS |
| TAGGTATTGGAGTTTGTATA+GGG | 0.340867 | 6:-38963282 | MsG0680032349.01.T01:CDS |
| CATCTACATTGCATTTATAT+AGG | 0.398019 | 6:+38963332 | None:intergenic |
| TGATAGAGGATAGTATTTAC+AGG | 0.403545 | 6:-38963105 | MsG0680032349.01.T01:CDS |
| TAAACTATAAGCAACATTGT+TGG | 0.433080 | 6:+38962979 | None:intergenic |
| TCCCTCAACAAGGTAGGTAT+TGG | 0.435574 | 6:-38963295 | MsG0680032349.01.T01:CDS |
| ACCTTATTGGATGTGGATAT+GGG | 0.459937 | 6:-38963213 | MsG0680032349.01.T01:CDS |
| AACAGATTCCAATGTGATAG+AGG | 0.469577 | 6:-38963119 | MsG0680032349.01.T01:CDS |
| CATTTATATAGGTCGGCACT+TGG | 0.488393 | 6:+38963343 | None:intergenic |
| ACTTCATTGTGAAGTACATT+CGG | 0.491675 | 6:-38963009 | MsG0680032349.01.T01:CDS |
| TACATTGCATTTATATAGGT+CGG | 0.494113 | 6:+38963336 | None:intergenic |
| TCGTTAAATCTGTGACCATA+AGG | 0.495673 | 6:+38963032 | None:intergenic |
| GATCAAAACCTTATTGGATG+TGG | 0.506320 | 6:-38963220 | MsG0680032349.01.T01:CDS |
| TCCAATACCTACCTTGTTGA+GGG | 0.509656 | 6:+38963294 | None:intergenic |
| GTCTCTTATATGTCATGCAA+TGG | 0.516389 | 6:-38963177 | MsG0680032349.01.T01:CDS |
| TCTCTTATATGTCATGCAAT+GGG | 0.517951 | 6:-38963176 | MsG0680032349.01.T01:CDS |
| AGTATTTACAGGGGTAGTGA+TGG | 0.523954 | 6:-38963094 | MsG0680032349.01.T01:CDS |
| CTCCAATACCTACCTTGTTG+AGG | 0.550011 | 6:+38963293 | None:intergenic |
| GTTTGTATAGGGGATAAAGA+AGG | 0.574871 | 6:-38963271 | MsG0680032349.01.T01:CDS |
| TCTAATTCCCTCAACAAGGT+AGG | 0.577257 | 6:-38963301 | MsG0680032349.01.T01:CDS |
| AATACTATCCTCTATCACAT+TGG | 0.580540 | 6:+38963111 | None:intergenic |
| AGGTATTGGAGTTTGTATAG+GGG | 0.585290 | 6:-38963281 | MsG0680032349.01.T01:CDS |
| GATAGAGGATAGTATTTACA+GGG | 0.591196 | 6:-38963104 | MsG0680032349.01.T01:CDS |
| AGAATGGCGGTATCGAGTAG+AGG | 0.641497 | 6:-38963409 | MsG0680032349.01.T01:CDS |
| AGTTGAAAACTTGCATGACA+CGG | 0.649761 | 6:+38962944 | None:intergenic |
| ATGTTGCTTATAGTTTAACG+AGG | 0.684943 | 6:-38962973 | MsG0680032349.01.T01:CDS |
| ATAGAGGATAGTATTTACAG+GGG | 0.688554 | 6:-38963103 | MsG0680032349.01.T01:CDS |
| TGTTGCTTATAGTTTAACGA+GGG | 0.702670 | 6:-38962972 | MsG0680032349.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATCATAATATGATGAATA+TGG | - | Chr6:38963122-38963141 | MsG0680032349.01.T01:CDS | 20.0% |
| !! | TGTCTTAACAAAAAACATAA+TGG | - | Chr6:38963029-38963048 | MsG0680032349.01.T01:CDS | 20.0% |
| ! | CATCTACATTGCATTTATAT+AGG | + | Chr6:38962942-38962961 | None:intergenic | 25.0% |
| ! | TAAACTATAAGCAACATTGT+TGG | + | Chr6:38963295-38963314 | None:intergenic | 25.0% |
| ! | TACATTGCATTTATATAGGT+CGG | + | Chr6:38962938-38962957 | None:intergenic | 25.0% |
| !!! | ATAATGGATCAAAACCTTAT+TGG | - | Chr6:38963045-38963064 | MsG0680032349.01.T01:CDS | 25.0% |
| AATACTATCCTCTATCACAT+TGG | + | Chr6:38963163-38963182 | None:intergenic | 30.0% | |
| ACTTCATTGTGAAGTACATT+CGG | - | Chr6:38963262-38963281 | MsG0680032349.01.T01:CDS | 30.0% | |
| ATAGAGGATAGTATTTACAG+GGG | - | Chr6:38963168-38963187 | MsG0680032349.01.T01:CDS | 30.0% | |
| GATAGAGGATAGTATTTACA+GGG | - | Chr6:38963167-38963186 | MsG0680032349.01.T01:CDS | 30.0% | |
| TAAAGATTGTAGACACCTTA+TGG | - | Chr6:38963224-38963243 | MsG0680032349.01.T01:CDS | 30.0% | |
| TCTCTTATATGTCATGCAAT+GGG | - | Chr6:38963095-38963114 | MsG0680032349.01.T01:CDS | 30.0% | |
| TGATAGAGGATAGTATTTAC+AGG | - | Chr6:38963166-38963185 | MsG0680032349.01.T01:CDS | 30.0% | |
| ! | ATGTTGCTTATAGTTTAACG+AGG | - | Chr6:38963298-38963317 | MsG0680032349.01.T01:CDS | 30.0% |
| ! | TGTTGCTTATAGTTTAACGA+GGG | - | Chr6:38963299-38963318 | MsG0680032349.01.T01:CDS | 30.0% |
| ! | TTTTTCTAATTCCCTCAACA+AGG | - | Chr6:38962966-38962985 | MsG0680032349.01.T01:CDS | 30.0% |
| !! | TAGGTATTGGAGTTTGTATA+GGG | - | Chr6:38962989-38963008 | MsG0680032349.01.T01:CDS | 30.0% |
| AACAGATTCCAATGTGATAG+AGG | - | Chr6:38963152-38963171 | MsG0680032349.01.T01:CDS | 35.0% | |
| AACCTTATTGGATGTGGATA+TGG | - | Chr6:38963057-38963076 | MsG0680032349.01.T01:CDS | 35.0% | |
| ACCTTATTGGATGTGGATAT+GGG | - | Chr6:38963058-38963077 | MsG0680032349.01.T01:CDS | 35.0% | |
| AGTTGAAAACTTGCATGACA+CGG | + | Chr6:38963330-38963349 | None:intergenic | 35.0% | |
| GATCAAAACCTTATTGGATG+TGG | - | Chr6:38963051-38963070 | MsG0680032349.01.T01:CDS | 35.0% | |
| GTCTCTTATATGTCATGCAA+TGG | - | Chr6:38963094-38963113 | MsG0680032349.01.T01:CDS | 35.0% | |
| GTTGAGGGAATTAGAAAAAG+AGG | + | Chr6:38962965-38962984 | None:intergenic | 35.0% | |
| GTTTGTATAGGGGATAAAGA+AGG | - | Chr6:38963000-38963019 | MsG0680032349.01.T01:CDS | 35.0% | |
| TCGTTAAATCTGTGACCATA+AGG | + | Chr6:38963242-38963261 | None:intergenic | 35.0% | |
| !! | AGGTATTGGAGTTTGTATAG+GGG | - | Chr6:38962990-38963009 | MsG0680032349.01.T01:CDS | 35.0% |
| !! | GTAGGTATTGGAGTTTGTAT+AGG | - | Chr6:38962988-38963007 | MsG0680032349.01.T01:CDS | 35.0% |
| AGTATTTACAGGGGTAGTGA+TGG | - | Chr6:38963177-38963196 | MsG0680032349.01.T01:CDS | 40.0% | |
| CATTTATATAGGTCGGCACT+TGG | + | Chr6:38962931-38962950 | None:intergenic | 40.0% | |
| TCCAATACCTACCTTGTTGA+GGG | + | Chr6:38962980-38962999 | None:intergenic | 40.0% | |
| TCCCATATCCACATCCAATA+AGG | + | Chr6:38963062-38963081 | None:intergenic | 40.0% | |
| TCTAATTCCCTCAACAAGGT+AGG | - | Chr6:38962970-38962989 | MsG0680032349.01.T01:CDS | 40.0% | |
| CTCCAATACCTACCTTGTTG+AGG | + | Chr6:38962981-38963000 | None:intergenic | 45.0% | |
| TCCCTCAACAAGGTAGGTAT+TGG | - | Chr6:38962976-38962995 | MsG0680032349.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 38962865 | 38963428 | 38962865 | ID=MsG0680032349.01;Name=MsG0680032349.01 |
| Chr6 | mRNA | 38962865 | 38963428 | 38962865 | ID=MsG0680032349.01.T01;Parent=MsG0680032349.01;Name=MsG0680032349.01.T01;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|0|1|0|187 |
| Chr6 | exon | 38962865 | 38963428 | 38962865 | ID=MsG0680032349.01.T01:exon:21217;Parent=MsG0680032349.01.T01 |
| Chr6 | CDS | 38962865 | 38963428 | 38962865 | ID=MsG0680032349.01.T01:cds;Parent=MsG0680032349.01.T01 |
| Gene Sequence |
| Protein sequence |
