Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032493.01.T01 | KAH0998132.1 | 90.909 | 66 | 6 | 0 | 1 | 66 | 18 | 83 | 1.98E-35 | 124 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032493.01.T01 | P46818 | 92.424 | 66 | 5 | 0 | 1 | 66 | 1013 | 1078 | 2.12E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032493.01.T01 | A0A1R3KZG8 | 90.909 | 66 | 6 | 0 | 1 | 66 | 18 | 83 | 9.47e-36 | 124 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380016059.01 | MsG0680032493.01 | 0.823085 | 1.674670e-53 | 1.427738e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032493.01.T01 | MTR_4g051130 | 98.462 | 65 | 1 | 0 | 1 | 65 | 992 | 1056 | 2.31e-37 | 131 |
| MsG0680032493.01.T01 | MTR_0002s0510 | 73.333 | 60 | 16 | 0 | 1 | 60 | 1063 | 1122 | 5.28e-23 | 90.5 |
| MsG0680032493.01.T01 | MTR_7g052990 | 87.500 | 32 | 4 | 0 | 1 | 32 | 34 | 65 | 7.73e-13 | 57.4 |
| MsG0680032493.01.T01 | MTR_7g052900 | 87.500 | 32 | 4 | 0 | 1 | 32 | 34 | 65 | 8.25e-13 | 57.0 |
| MsG0680032493.01.T01 | MTR_5g088810 | 81.250 | 32 | 6 | 0 | 1 | 32 | 34 | 65 | 5.70e-11 | 52.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032493.01.T01 | ATCG00190 | 92.424 | 66 | 5 | 0 | 1 | 66 | 1007 | 1072 | 2.46e-35 | 125 |
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTTCCTTCCTTTGAATC+TGG | 0.340714 | 6:-42757936 | None:intergenic |
| TCTGGAGCATCCACAGGTTT+AGG | 0.340865 | 6:-42757835 | None:intergenic |
| ACGAGCAATCGAAAAGATTC+TGG | 0.342745 | 6:-42757853 | None:intergenic |
| GTACTCGGAACTACGATTAT+TGG | 0.356639 | 6:+42757802 | MsG0680032493.01.T01:CDS |
| CGTTCGAGAACTACGATCTT+TGG | 0.364953 | 6:+42757873 | MsG0680032493.01.T01:CDS |
| AGAACTACGATCTTTGGCTC+TGG | 0.513829 | 6:+42757879 | MsG0680032493.01.T01:CDS |
| AAAGATTCTGGAGCATCCAC+AGG | 0.531781 | 6:-42757841 | None:intergenic |
| AGAAAGACTTCCAGATTCAA+AGG | 0.540462 | 6:+42757926 | MsG0680032493.01.T01:CDS |
| CTCGGAACTACGATTATTGG+AGG | 0.559077 | 6:+42757805 | MsG0680032493.01.T01:CDS |
| AGACTTCCAGATTCAAAGGA+AGG | 0.563377 | 6:+42757930 | MsG0680032493.01.T01:CDS |
| GGAAGTCTTTCTCATATACA+AGG | 0.586003 | 6:-42757915 | None:intergenic |
| AGGAACAATACCTAAACCTG+TGG | 0.656849 | 6:+42757825 | MsG0680032493.01.T01:CDS |
| AAAGCTCGTCAAGAAGTACT+CGG | 0.659322 | 6:+42757787 | MsG0680032493.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTTCCTTCCTTTGAATC+TGG | - | Chr6:42757939-42757958 | None:intergenic | 35.0% | |
| AGAAAGACTTCCAGATTCAA+AGG | + | Chr6:42757926-42757945 | MsG0680032493.01.T01:CDS | 35.0% | |
| GGAAGTCTTTCTCATATACA+AGG | - | Chr6:42757918-42757937 | None:intergenic | 35.0% | |
| ACGAGCAATCGAAAAGATTC+TGG | - | Chr6:42757856-42757875 | None:intergenic | 40.0% | |
| AGACTTCCAGATTCAAAGGA+AGG | + | Chr6:42757930-42757949 | MsG0680032493.01.T01:CDS | 40.0% | |
| AGGAACAATACCTAAACCTG+TGG | + | Chr6:42757825-42757844 | MsG0680032493.01.T01:CDS | 40.0% | |
| GTACTCGGAACTACGATTAT+TGG | + | Chr6:42757802-42757821 | MsG0680032493.01.T01:CDS | 40.0% | |
| !! | AAAGCTCGTCAAGAAGTACT+CGG | + | Chr6:42757787-42757806 | MsG0680032493.01.T01:CDS | 40.0% |
| CGTTCGAGAACTACGATCTT+TGG | + | Chr6:42757873-42757892 | MsG0680032493.01.T01:CDS | 45.0% | |
| CTCGGAACTACGATTATTGG+AGG | + | Chr6:42757805-42757824 | MsG0680032493.01.T01:CDS | 45.0% | |
| ! | AGAACTACGATCTTTGGCTC+TGG | + | Chr6:42757879-42757898 | MsG0680032493.01.T01:CDS | 45.0% |
| !! | AAAGATTCTGGAGCATCCAC+AGG | - | Chr6:42757844-42757863 | None:intergenic | 45.0% |
| ! | TCTGGAGCATCCACAGGTTT+AGG | - | Chr6:42757838-42757857 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 42757760 | 42757960 | 42757760 | ID=MsG0680032493.01;Name=MsG0680032493.01 |
| Chr6 | mRNA | 42757760 | 42757960 | 42757760 | ID=MsG0680032493.01.T01;Parent=MsG0680032493.01;Name=MsG0680032493.01.T01;_AED=0.47;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|66 |
| Chr6 | exon | 42757760 | 42757960 | 42757760 | ID=MsG0680032493.01.T01:exon:16213;Parent=MsG0680032493.01.T01 |
| Chr6 | CDS | 42757760 | 42757960 | 42757760 | ID=MsG0680032493.01.T01:cds;Parent=MsG0680032493.01.T01 |
| Gene Sequence |
| Protein sequence |
