Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016059.01.T01 | WP_156524861.1 | 91.304 | 46 | 4 | 0 | 1 | 46 | 3 | 48 | 7.33E-22 | 89 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016059.01.T01 | P05311 | 93.478 | 46 | 3 | 0 | 1 | 46 | 683 | 728 | 4.56E-22 | 90.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016059.01.T01 | B4ZXP2 | 91.304 | 46 | 4 | 0 | 1 | 46 | 12 | 57 | 3.71e-22 | 89.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015522.01 | MsG0380016059.01 | 0.824857 | 6.432286e-54 | 5.766947e-51 |
| MsG0380016059.01 | MsG0580024908.01 | 0.911465 | 5.895042e-83 | 1.264989e-78 |
| MsG0380016059.01 | MsG0680031180.01 | 0.829295 | 5.577161e-55 | 5.685280e-52 |
| MsG0380016059.01 | MsG0680032493.01 | 0.823085 | 1.674670e-53 | 1.427738e-50 |
| MsG0380016059.01 | MsG0680034123.01 | 0.839209 | 1.817184e-57 | 2.491905e-54 |
| MsG0380016059.01 | MsG0780035997.01 | 0.818356 | 2.045401e-52 | 1.527715e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016059.01.T01 | MTR_3g035600 | 93.478 | 46 | 3 | 0 | 1 | 46 | 1452 | 1497 | 4.58e-23 | 90.1 |
| MsG0380016059.01.T01 | MTR_0002s0580 | 91.304 | 46 | 4 | 0 | 1 | 46 | 1387 | 1432 | 1.40e-22 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016059.01.T01 | ATCG00340 | 89.130 | 46 | 5 | 0 | 1 | 46 | 683 | 728 | 4.58e-22 | 87.0 |
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGCTTGTTAGAAGCTT+TGG | 0.327277 | 3:+78498427 | MsG0380016058.01.T01:intron |
| TTCCATTGTGCAAGCAAGAT+TGG | 0.365278 | 3:-78498529 | MsG0380016059.01.T01:CDS |
| GAAGTTTACTTGTTGCTTGT+TGG | 0.406272 | 3:+78498449 | MsG0380016058.01.T01:intron |
| CATTGGCGAATTTGATTCGA+TGG | 0.443845 | 3:-78498572 | MsG0380016059.01.T01:CDS |
| ATATATAACATACCGAAAAG+TGG | 0.500782 | 3:+78498496 | MsG0380016058.01.T01:intron |
| ATTGTGCAAGCAAGATTGGT+TGG | 0.502433 | 3:-78498525 | MsG0380016059.01.T01:CDS |
| TGCACAATGGAAAGAGCCAC+TGG | 0.506849 | 3:+78498540 | MsG0380016058.01.T01:intron |
| ATGTTATATATTTACTTATG+CGG | 0.539295 | 3:-78498484 | MsG0380016059.01.T01:CDS |
| TCCAGATCAACGCACACCAT+TGG | 0.548583 | 3:-78498589 | None:intergenic |
| CATCGAATCAAATTCGCCAA+TGG | 0.605695 | 3:+78498573 | MsG0380016058.01.T01:CDS |
| AACCAATCTTGCTTGCACAA+TGG | 0.610391 | 3:+78498527 | MsG0380016058.01.T01:intron |
| TCGATGGAGAGATAAACCAG+TGG | 0.770539 | 3:-78498556 | MsG0380016059.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTATATATTTACTTATG+CGG | - | Chr3:78498535-78498554 | MsG0380016059.01.T01:CDS | 15.0% |
| ! | ATATATAACATACCGAAAAG+TGG | + | Chr3:78498526-78498545 | MsG0380016058.01.T01:intron | 25.0% |
| ! | GAAGTTTACTTGTTGCTTGT+TGG | + | Chr3:78498573-78498592 | MsG0380016058.01.T01:CDS | 35.0% |
| AACCAATCTTGCTTGCACAA+TGG | + | Chr3:78498495-78498514 | MsG0380016058.01.T01:intron | 40.0% | |
| ATTGTGCAAGCAAGATTGGT+TGG | - | Chr3:78498494-78498513 | MsG0380016059.01.T01:CDS | 40.0% | |
| CATCGAATCAAATTCGCCAA+TGG | + | Chr3:78498449-78498468 | MsG0380016058.01.T01:intron | 40.0% | |
| TTCCATTGTGCAAGCAAGAT+TGG | - | Chr3:78498490-78498509 | MsG0380016059.01.T01:CDS | 40.0% | |
| ! | CATTGGCGAATTTGATTCGA+TGG | - | Chr3:78498447-78498466 | MsG0380016059.01.T01:CDS | 40.0% |
| !!! | GGTTGGATTAGTCCACTTTT+CGG | - | Chr3:78498511-78498530 | MsG0380016059.01.T01:CDS | 40.0% |
| TCGATGGAGAGATAAACCAG+TGG | - | Chr3:78498463-78498482 | MsG0380016059.01.T01:CDS | 45.0% | |
| TGCACAATGGAAAGAGCCAC+TGG | + | Chr3:78498482-78498501 | MsG0380016058.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 78498437 | 78498604 | 78498437 | ID=MsG0380016059.01;Name=MsG0380016059.01 |
| Chr3 | mRNA | 78498437 | 78498604 | 78498437 | ID=MsG0380016059.01.T01;Parent=MsG0380016059.01;Name=MsG0380016059.01.T01;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|0|1|0|55 |
| Chr3 | exon | 78498437 | 78498604 | 78498437 | ID=MsG0380016059.01.T01:exon:17421;Parent=MsG0380016059.01.T01 |
| Chr3 | CDS | 78498437 | 78498604 | 78498437 | ID=MsG0380016059.01.T01:cds;Parent=MsG0380016059.01.T01 |
| Gene Sequence |
| Protein sequence |
