AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032519.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032519.01.T01 MTR_6g086510 93.137 102 7 0 37 138 115 216 2.26e-64 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032519.01.T01 AT5G19460 56.604 106 42 2 37 138 99 204 1.24e-31 116
MsG0680032519.01.T01 AT5G19470 55.340 103 41 2 37 138 97 195 4.48e-30 112
MsG0680032519.01.T01 AT5G19470 55.340 103 41 2 37 138 97 195 5.18e-30 111

Find 54 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGCTGCAGCTCCTTATTT+TGG 0.177594 6:-43589165 MsG0680032519.01.T01:CDS
TTGTACCTTATGCCAAAATA+AGG 0.269516 6:+43589153 None:intergenic
CGGTTGCAAAGTTGACTTTA+TGG 0.282555 6:+43593256 None:intergenic
TGGCTATGTAATCGAGCATT+TGG 0.324074 6:-43589377 MsG0680032519.01.T01:CDS
CCCAATGATGGAAGCCCCTT+TGG 0.334011 6:-43589457 MsG0680032519.01.T01:CDS
TTGATTTCGAAATTGCTTGT+AGG 0.349619 6:-43589690 MsG0680032519.01.T01:CDS
TGGTGCATGTTGCTATCTTT+TGG 0.370504 6:-43590085 MsG0680032519.01.T01:exon
TTGGGAGAGGAGCATATTCC+AGG 0.372454 6:-43589358 MsG0680032519.01.T01:CDS
GAAAGTGTGAGAGGGTTTGA+AGG 0.373970 6:+43593440 None:intergenic
AGAAGAAGAGAAGGGTTTGT+AGG 0.384314 6:+43593484 None:intergenic
TCGCAAGTCTTGTAACTTTG+CGG 0.386472 6:-43590041 MsG0680032519.01.T01:intron
ACCAAAGGGGCTTCCATCAT+TGG 0.391806 6:+43589456 None:intergenic
CATGTTTCTCAATCTGAAGT+TGG 0.408924 6:-43593321 MsG0680032519.01.T01:five_prime_UTR
GTCATTGAGGGCGACGTTGT+TGG 0.409677 6:-43589642 MsG0680032519.01.T01:CDS
GGGAAGAGTGTGGTTGTTGA+TGG 0.411141 6:+43593404 None:intergenic
TCCTCAGCTGTCTTCAGCAA+TGG 0.422425 6:+43589415 None:intergenic
GAAGGGTTTGTAGGAGAAGA+AGG 0.433689 6:+43593493 None:intergenic
GGCTATGTAATCGAGCATTT+GGG 0.440338 6:-43589376 MsG0680032519.01.T01:CDS
CGTTCTAATGAAAAGAAAAC+GGG 0.450446 6:+43589186 None:intergenic
AGGAGCATATTCCAGGTATA+CGG 0.452411 6:-43589351 MsG0680032519.01.T01:CDS
ATTCTTCCATTTGTCATTGA+GGG 0.458981 6:-43589654 MsG0680032519.01.T01:CDS
AATGGAAGAATTCCGATTGT+TGG 0.465129 6:+43589666 None:intergenic
AGGTTTGTTGAGCATTTAAG+AGG 0.470542 6:-43589502 MsG0680032519.01.T01:CDS
CCAAAGGGGCTTCCATCATT+GGG 0.472746 6:+43589457 None:intergenic
ACGTTCTAATGAAAAGAAAA+CGG 0.490303 6:+43589185 None:intergenic
ACGTCGCCCTCAATGACAAA+TGG 0.490482 6:+43589648 None:intergenic
TTGTCAAATGAGGACTTCAC+AGG 0.500036 6:+43589210 None:intergenic
AATGAAACACAGTGACCAAA+GGG 0.500047 6:+43589442 None:intergenic
AATTCTTCCATTTGTCATTG+AGG 0.503126 6:-43589655 MsG0680032519.01.T01:CDS
TTGTTGGATTCATTCACAAT+CGG 0.508086 6:-43589626 MsG0680032519.01.T01:intron
AATACCTCGTTCCGTATACC+TGG 0.509788 6:+43589340 None:intergenic
GATCCTTGTTCTTATCTACA+AGG 0.513032 6:-43593285 MsG0680032519.01.T01:five_prime_UTR
TGATGGAGAGAAAGTGTGAG+AGG 0.513608 6:+43593431 None:intergenic
ACTGGTGGCTGAAGAAGAAA+AGG 0.525903 6:+43593380 None:intergenic
GAGTGTGCACGTGCTGCCAC+TGG 0.531569 6:+43593362 None:intergenic
AAGAAAAGGAGGGAAGAGTG+TGG 0.538836 6:+43593394 None:intergenic
GCAGCACGTGCACACTCACA+TGG 0.546676 6:-43593356 MsG0680032519.01.T01:five_prime_UTR
GATGGAGAGAAAGTGTGAGA+GGG 0.556532 6:+43593432 None:intergenic
GATTACATAGCCAAGCGCGC+TGG 0.567603 6:+43589387 None:intergenic
TTGCTTGTAGGACCAACAAT+CGG 0.568803 6:-43589678 MsG0680032519.01.T01:CDS
TGTGCACGTGCTGCCACTGG+TGG 0.568995 6:+43593365 None:intergenic
GAAAACGGGTTTGTCAAATG+AGG 0.582709 6:+43589200 None:intergenic
CAATGAAACACAGTGACCAA+AGG 0.586554 6:+43589441 None:intergenic
TGTAATCGAGCATTTGGGAG+AGG 0.600564 6:-43589371 MsG0680032519.01.T01:CDS
GGTAAAGAACTCACAGAACA+CGG 0.615286 6:+43593236 None:intergenic
TGGTTGTTGATGGAAGATGA+TGG 0.632740 6:+43593414 None:intergenic
GGTGGCTGAAGAAGAAAAGG+AGG 0.638422 6:+43593383 None:intergenic
TCCATTGCTGAAGACAGCTG+AGG 0.673124 6:-43589416 MsG0680032519.01.T01:CDS
CAGCACGTGCACACTCACAT+GGG 0.680877 6:-43593355 MsG0680032519.01.T01:five_prime_UTR
TAACCTTGTAGATAAGAACA+AGG 0.682039 6:+43593282 None:intergenic
TGTCAAATGAGGACTTCACA+GGG 0.682552 6:+43589211 None:intergenic
GTGGCTGAAGAAGAAAAGGA+GGG 0.700450 6:+43593384 None:intergenic
TATTCCAGGTATACGGAACG+AGG 0.728323 6:-43589344 MsG0680032519.01.T01:intron
ATGAAACACAGTGACCAAAG+GGG 0.740460 6:+43589443 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCAACTCAATTTATAT+AGG - Chr6:43591030-43591049 MsG0680032519.01.T01:intron 15.0%
!!! ACTTTTCATTAAGATTTTTT+TGG - Chr6:43592874-43592893 MsG0680032519.01.T01:intron 15.0%
!!! ATTTTAAGTTATTAACTGTA+AGG - Chr6:43590664-43590683 MsG0680032519.01.T01:intron 15.0%
!!! GTTTTTTTTATCATTTATGT+TGG - Chr6:43590026-43590045 MsG0680032519.01.T01:intron 15.0%
!!! TATCATTAGTTTTGAAATTT+GGG - Chr6:43590386-43590405 MsG0680032519.01.T01:intron 15.0%
!!! TATTTTTATCAATTTCTGTT+TGG - Chr6:43589710-43589729 MsG0680032519.01.T01:intron 15.0%
!!! TGATTTTATAAAGTTGATTT+TGG - Chr6:43589930-43589949 MsG0680032519.01.T01:intron 15.0%
!!! TGATTTTTATCAATTTTGTT+TGG - Chr6:43589561-43589580 MsG0680032519.01.T01:intron 15.0%
!! AACAAATTACAATAACCAAA+AGG + Chr6:43590545-43590564 None:intergenic 20.0%
!! AACAAGACAATCTATTTAAA+AGG + Chr6:43592954-43592973 None:intergenic 20.0%
!! AATGTAAAGTTGTTTATGTT+TGG - Chr6:43589520-43589539 MsG0680032519.01.T01:intron 20.0%
!! AATTCATATTATCCAAAGTT+TGG + Chr6:43590212-43590231 None:intergenic 20.0%
!! ACTTTACATTCAAATCAATT+CGG + Chr6:43589510-43589529 None:intergenic 20.0%
!! ACTTTATCTTCAAATCAATT+CGG + Chr6:43590135-43590154 None:intergenic 20.0%
!! ACTTTATTGAAAAAAAGAAG+TGG + Chr6:43590345-43590364 None:intergenic 20.0%
!! ATTCTAAACTTGATTCAAAA+TGG + Chr6:43592762-43592781 None:intergenic 20.0%
!! GTTGTGAGTTTAATAATATA+GGG + Chr6:43590480-43590499 None:intergenic 20.0%
!! TAAGATTGGATTTAATTGAT+TGG - Chr6:43592309-43592328 MsG0680032519.01.T01:intron 20.0%
!! TATAAGGAAAAATGAAGAAA+GGG + Chr6:43593073-43593092 None:intergenic 20.0%
!! TGGATATATTGAACTAAAAT+TGG - Chr6:43589730-43589749 MsG0680032519.01.T01:intron 20.0%
!! TGTTGTGAGTTTAATAATAT+AGG + Chr6:43590481-43590500 None:intergenic 20.0%
!! TTATAAGGAAAAATGAAGAA+AGG + Chr6:43593074-43593093 None:intergenic 20.0%
!! TTGTGAGTTTAATAATATAG+GGG + Chr6:43590479-43590498 None:intergenic 20.0%
!!! AGTGTTAATATTTTGATGTT+GGG - Chr6:43592534-43592553 MsG0680032519.01.T01:intron 20.0%
!!! ATTAACCTCTAATAGATTTT+TGG - Chr6:43592780-43592799 MsG0680032519.01.T01:intron 20.0%
!!! GTATCATTAGTTTTGAAATT+TGG - Chr6:43590385-43590404 MsG0680032519.01.T01:intron 20.0%
!!! TACGAAAAAGTGATTTTTAT+GGG - Chr6:43590173-43590192 MsG0680032519.01.T01:intron 20.0%
!!! TACTCGTATTTAATTTCATA+AGG - Chr6:43591278-43591297 MsG0680032519.01.T01:intron 20.0%
!!! TAGTGTTAATATTTTGATGT+TGG - Chr6:43592533-43592552 MsG0680032519.01.T01:intron 20.0%
!!! TGATTATGACATTGTATTTT+TGG - Chr6:43589975-43589994 MsG0680032519.01.T01:intron 20.0%
!!! TGATTTTTATGGGTTTTATT+TGG - Chr6:43590183-43590202 MsG0680032519.01.T01:intron 20.0%
!!! TTACGAAAAAGTGATTTTTA+TGG - Chr6:43590172-43590191 MsG0680032519.01.T01:intron 20.0%
!!! TTTAATTTCATAAGGAAGTT+AGG - Chr6:43591286-43591305 MsG0680032519.01.T01:intron 20.0%
!!! TTTGGTTATTGTAATTTGTT+GGG - Chr6:43590545-43590564 MsG0680032519.01.T01:intron 20.0%
!!! TTTTCAAATACTTAAAGTCA+TGG + Chr6:43592816-43592835 None:intergenic 20.0%
!!! TTTTGGTTATTGTAATTTGT+TGG - Chr6:43590544-43590563 MsG0680032519.01.T01:intron 20.0%
! AAAGTTCAACTTTGTAATTG+TGG - Chr6:43589828-43589847 MsG0680032519.01.T01:intron 25.0%
! AAGATATTATAAGACACTCT+CGG + Chr6:43591773-43591792 None:intergenic 25.0%
! AAGTTATTAACTGTAAGGTT+AGG - Chr6:43590669-43590688 MsG0680032519.01.T01:intron 25.0%
! AATTGATATTTCAGGCATAT+TGG + Chr6:43591123-43591142 None:intergenic 25.0%
! ACAATGATCACTGATAAAAT+AGG - Chr6:43590498-43590517 MsG0680032519.01.T01:intron 25.0%
! ACGTTCTAATGAAAAGAAAA+CGG + Chr6:43593480-43593499 None:intergenic 25.0%
! AGGAGTTATTTATCATTTGA+TGG - Chr6:43592508-43592527 MsG0680032519.01.T01:intron 25.0%
! ATTCTCAGTCAAAAAAGAAA+AGG + Chr6:43590274-43590293 None:intergenic 25.0%
! ATTGGATTTAATTGATTGGA+CGG - Chr6:43592313-43592332 MsG0680032519.01.T01:intron 25.0%
! ATTGTCCAAAAATCTATTAG+AGG + Chr6:43592788-43592807 None:intergenic 25.0%
! CAGTTTAAATATCTTGTGAA+TGG - Chr6:43591886-43591905 MsG0680032519.01.T01:intron 25.0%
! GCTACTTATATGCTAAATTA+AGG + Chr6:43590916-43590935 None:intergenic 25.0%
! GTAGAAATTTAGAGAGAAAA+TGG - Chr6:43591911-43591930 MsG0680032519.01.T01:intron 25.0%
! GTTAATGTGAATGATGTTAA+GGG + Chr6:43592740-43592759 None:intergenic 25.0%
! TGTGTGTGTGTAATAAATTT+GGG - Chr6:43589758-43589777 MsG0680032519.01.T01:intron 25.0%
! TTATGCGAAAATTCACAATT+GGG + Chr6:43590322-43590341 None:intergenic 25.0%
! TTCTCAGTCAAAAAAGAAAA+GGG + Chr6:43590273-43590292 None:intergenic 25.0%
!! TTTATTTGGAATCCAAACTT+TGG - Chr6:43590197-43590216 MsG0680032519.01.T01:intron 25.0%
!!! ACTATTTTGACTGAGTAATT+CGG + Chr6:43591588-43591607 None:intergenic 25.0%
!!! ATATTAGCTTTTGTATGCTT+TGG - Chr6:43590083-43590102 MsG0680032519.01.T01:exon 25.0%
!!! ATCTAATGTCCTACTTTTTT+GGG + Chr6:43590782-43590801 None:intergenic 25.0%
!!! GAATTGATTTGAAGATAAAG+TGG - Chr6:43590134-43590153 MsG0680032519.01.T01:intron 25.0%
!!! GTGTTAATATTTTGATGTTG+GGG - Chr6:43592535-43592554 MsG0680032519.01.T01:intron 25.0%
!!! GTTAAACTTTGAAATTGTTC+AGG - Chr6:43593140-43593159 MsG0680032519.01.T01:intron 25.0%
!!! TAGAAATATTAGCTTTTAGC+TGG - Chr6:43589625-43589644 MsG0680032519.01.T01:intron 25.0%
!!! TATGGATTCTAGTTTTTGAT+GGG - Chr6:43591315-43591334 MsG0680032519.01.T01:intron 25.0%
!!! TTACTCTTTTTTTTGCTGAT+GGG - Chr6:43592362-43592381 MsG0680032519.01.T01:intron 25.0%
!!! TTTTCTTTTTTGACTGAGAA+TGG - Chr6:43590273-43590292 MsG0680032519.01.T01:intron 25.0%
AAAACACAGACAAGAAATGT+AGG + Chr6:43593421-43593440 None:intergenic 30.0%
AAAATTAACTTTACACCTGC+AGG + Chr6:43592931-43592950 None:intergenic 30.0%
AATTCTTCCATTTGTCATTG+AGG - Chr6:43593007-43593026 MsG0680032519.01.T01:intron 30.0%
ACACAAAAGAGAGGTAATTA+AGG + Chr6:43589450-43589469 None:intergenic 30.0%
ACATTGAGCTTTCATTCTAT+CGG - Chr6:43590826-43590845 MsG0680032519.01.T01:intron 30.0%
AGAAGGAGATTGTTTAAATG+AGG - Chr6:43591468-43591487 MsG0680032519.01.T01:intron 30.0%
AGATATTATAAGACACTCTC+GGG + Chr6:43591772-43591791 None:intergenic 30.0%
AGTCGTGTAAAACATAACTA+TGG - Chr6:43591083-43591102 MsG0680032519.01.T01:intron 30.0%
ATTCTTCCATTTGTCATTGA+GGG - Chr6:43593008-43593027 MsG0680032519.01.T01:intron 30.0%
CAAAAAAGTAGGACATTAGA+TGG - Chr6:43590781-43590800 MsG0680032519.01.T01:intron 30.0%
CATTCAATGAAAGAGAATGA+TGG + Chr6:43593378-43593397 None:intergenic 30.0%
CGTTCTAATGAAAAGAAAAC+GGG + Chr6:43593479-43593498 None:intergenic 30.0%
CTGCTAAATTGTTTCATGAA+AGG + Chr6:43591361-43591380 None:intergenic 30.0%
GGGAAAGTAATTGATATTTC+AGG + Chr6:43591131-43591150 None:intergenic 30.0%
GGTTAATGTGAATGATGTTA+AGG + Chr6:43592741-43592760 None:intergenic 30.0%
GTCGCTAAATAAAGACATTT+CGG - Chr6:43591393-43591412 MsG0680032519.01.T01:intron 30.0%
GTGTGTGTGTGTAATAAATT+TGG - Chr6:43589757-43589776 MsG0680032519.01.T01:intron 30.0%
GTTATGCGAAAATTCACAAT+TGG + Chr6:43590323-43590342 None:intergenic 30.0%
TAAAATCTAAACCCAAATGC+TGG + Chr6:43590973-43590992 None:intergenic 30.0%
TAACCTTGTAGATAAGAACA+AGG + Chr6:43589383-43589402 None:intergenic 30.0%
TATGACACAAGCATCTTATA+AGG + Chr6:43593089-43593108 None:intergenic 30.0%
TATGGTTACATTGAAAACGA+AGG - Chr6:43591671-43591690 MsG0680032519.01.T01:intron 30.0%
TCACAAAAGAGAGGTAATTA+CGG + Chr6:43589872-43589891 None:intergenic 30.0%
TGAAGATAAAGTGGTATCTT+TGG - Chr6:43590143-43590162 MsG0680032519.01.T01:intron 30.0%
TGTGGATTTGATCGTTAAAA+TGG - Chr6:43591818-43591837 MsG0680032519.01.T01:intron 30.0%
TTGATTTCGAAATTGCTTGT+AGG - Chr6:43592972-43592991 MsG0680032519.01.T01:intron 30.0%
TTGGATTTAATTGATTGGAC+GGG - Chr6:43592314-43592333 MsG0680032519.01.T01:intron 30.0%
TTGTACCTTATGCCAAAATA+AGG + Chr6:43593512-43593531 None:intergenic 30.0%
TTGTTGGATTCATTCACAAT+CGG - Chr6:43593036-43593055 MsG0680032519.01.T01:intron 30.0%
TTTCCAAACAAACAACACAT+TGG + Chr6:43591952-43591971 None:intergenic 30.0%
! ACATCGTATAGTTTTCTTGT+CGG - Chr6:43592211-43592230 MsG0680032519.01.T01:intron 30.0%
! AGATAGTCATCTTTTTGTGT+AGG - Chr6:43591733-43591752 MsG0680032519.01.T01:intron 30.0%
! ATAGATTGTGTTATTGAGTC+TGG - Chr6:43590604-43590623 MsG0680032519.01.T01:intron 30.0%
! GCATGTGATTTTCATCATTT+TGG + Chr6:43589136-43589155 None:intergenic 30.0%
! TAAAGTGCAATTAAGCCTTT+TGG - Chr6:43590527-43590546 MsG0680032519.01.T01:intron 30.0%
!! GCTAGTTATTTCCCTTTTAA+CGG - Chr6:43590861-43590880 MsG0680032519.01.T01:intron 30.0%
!!! AGTTTATTGCCCCATTTTTT+CGG - Chr6:43591793-43591812 MsG0680032519.01.T01:intron 30.0%
!!! CATCTAATGTCCTACTTTTT+TGG + Chr6:43590783-43590802 None:intergenic 30.0%
!!! CTATTTTGACTGAGTAATTC+GGG + Chr6:43591587-43591606 None:intergenic 30.0%
!!! GAAATTGGTTTTGTTTAGGA+TGG - Chr6:43592557-43592576 MsG0680032519.01.T01:intron 30.0%
!!! GTATGGATTCTAGTTTTTGA+TGG - Chr6:43591314-43591333 MsG0680032519.01.T01:intron 30.0%
!!! GTCTCTTAATTTTGAAGTGA+AGG - Chr6:43590408-43590427 MsG0680032519.01.T01:intron 30.0%
!!! GTTACTCTTTTTTTTGCTGA+TGG - Chr6:43592361-43592380 MsG0680032519.01.T01:intron 30.0%
!!! TATTTTGATGTTGGGGAAAT+TGG - Chr6:43592542-43592561 MsG0680032519.01.T01:intron 30.0%
!!! TTACTGTTTTTGTCTGTCTT+GGG - Chr6:43592035-43592054 MsG0680032519.01.T01:intron 30.0%
!!! TTTACTGTTTTTGTCTGTCT+TGG - Chr6:43592034-43592053 MsG0680032519.01.T01:intron 30.0%
AAAGGGAAATAACTAGCATC+AGG + Chr6:43590858-43590877 None:intergenic 35.0%
AAATCCACACACCGAAAAAA+TGG + Chr6:43591807-43591826 None:intergenic 35.0%
AATCCACACACCGAAAAAAT+GGG + Chr6:43591806-43591825 None:intergenic 35.0%
AATGAAACACAGTGACCAAA+GGG + Chr6:43593223-43593242 None:intergenic 35.0%
AATGGAAGAATTCCGATTGT+TGG + Chr6:43592999-43593018 None:intergenic 35.0%
ACAGACAAGAAATGTAGGTT+AGG + Chr6:43593416-43593435 None:intergenic 35.0%
AGAAGAAGAAGAAGAAGAGA+AGG + Chr6:43589190-43589209 None:intergenic 35.0%
ATAGTGTATTCTGTGTTGAG+TGG - Chr6:43591603-43591622 MsG0680032519.01.T01:intron 35.0%
ATGCCATATTTCACGTTTCT+TGG + Chr6:43590717-43590736 None:intergenic 35.0%
CAGCGTAAGTTTATTGAGAA+GGG - Chr6:43592385-43592404 MsG0680032519.01.T01:intron 35.0%
GAAAACGATAGTTGAGTTTG+AGG - Chr6:43591549-43591568 MsG0680032519.01.T01:intron 35.0%
GAAGAAGAAGAAGAAGAGAA+GGG + Chr6:43589189-43589208 None:intergenic 35.0%
GAGAGAAAATGGCTGTTATT+TGG - Chr6:43591922-43591941 MsG0680032519.01.T01:intron 35.0%
GATCCTTGTTCTTATCTACA+AGG - Chr6:43589377-43589396 MsG0680032519.01.T01:CDS 35.0%
GTAAGTTTATTGAGAAGGGT+TGG - Chr6:43592389-43592408 MsG0680032519.01.T01:intron 35.0%
GTTTAAATGAGGAGAAGACT+TGG - Chr6:43591479-43591498 MsG0680032519.01.T01:intron 35.0%
TAGTGTATTCTGTGTTGAGT+GGG - Chr6:43591604-43591623 MsG0680032519.01.T01:intron 35.0%
TCTCCAAGAAACGTGAAATA+TGG - Chr6:43590711-43590730 MsG0680032519.01.T01:intron 35.0%
TTCATAAGGAAGTTAGGCAA+TGG - Chr6:43591292-43591311 MsG0680032519.01.T01:intron 35.0%
TTTCTGATGCAGATTTGTCT+CGG - Chr6:43592245-43592264 MsG0680032519.01.T01:intron 35.0%
! AGTCATCTTTTTGTGTAGGT+GGG - Chr6:43591737-43591756 MsG0680032519.01.T01:intron 35.0%
! CATGTTTCTCAATCTGAAGT+TGG - Chr6:43589341-43589360 MsG0680032519.01.T01:intron 35.0%
! TACCTCTCTTTTGTGAGTTT+GGG - Chr6:43589876-43589895 MsG0680032519.01.T01:intron 35.0%
! TAGTCATCTTTTTGTGTAGG+TGG - Chr6:43591736-43591755 MsG0680032519.01.T01:intron 35.0%
! TTACCTCTCTTTTGTGAGTT+TGG - Chr6:43589875-43589894 MsG0680032519.01.T01:intron 35.0%
! TTCGGAAATTTGAAGCTCTA+TGG - Chr6:43591653-43591672 MsG0680032519.01.T01:intron 35.0%
!! ACTCCAATGTGTTGTTTGTT+TGG - Chr6:43591946-43591965 MsG0680032519.01.T01:intron 35.0%
!! AGGTTTGTTGAGCATTTAAG+AGG - Chr6:43593160-43593179 MsG0680032519.01.T01:intron 35.0%
!! GCAGTTTTGATCCTTTGTTT+AGG - Chr6:43592283-43592302 MsG0680032519.01.T01:intron 35.0%
!! TCTTTTGTGAGTTTGGGTTT+CGG - Chr6:43589882-43589901 MsG0680032519.01.T01:intron 35.0%
!! TGAAAACGAAGGTTCTAGAA+TGG - Chr6:43591682-43591701 MsG0680032519.01.T01:intron 35.0%
!! TTGAGTCTGGAATTGCTTTT+AGG - Chr6:43590617-43590636 MsG0680032519.01.T01:intron 35.0%
!!! AATGTCCTACTTTTTTGGGT+TGG + Chr6:43590778-43590797 None:intergenic 35.0%
!!! ATGTCCTACTTTTTTGGGTT+GGG + Chr6:43590777-43590796 None:intergenic 35.0%
!!! CGAGCTAAGAGCATTTTTTT+TGG - Chr6:43592443-43592462 MsG0680032519.01.T01:intron 35.0%
!!! GAGTGTATGCTTGGTTTTAA+GGG - Chr6:43589655-43589674 MsG0680032519.01.T01:CDS 35.0%
!!! GTTTTTTTCGCTTAGCTCAT+AGG - Chr6:43592488-43592507 MsG0680032519.01.T01:intron 35.0%
!!! TGAGTGTATGCTTGGTTTTA+AGG - Chr6:43589654-43589673 MsG0680032519.01.T01:CDS 35.0%
!!! TGGGGAAATTGGTTTTGTTT+AGG - Chr6:43592553-43592572 MsG0680032519.01.T01:intron 35.0%
AAAAAGAGTAACCATGCGCA+AGG + Chr6:43592353-43592372 None:intergenic 40.0%
AACCCAAACTCACAAAAGAG+AGG + Chr6:43589881-43589900 None:intergenic 40.0%
AAGATCAACAACCAAGCACA+AGG + Chr6:43592686-43592705 None:intergenic 40.0%
AAGGAAGTTAGGCAATGGTA+TGG - Chr6:43591297-43591316 MsG0680032519.01.T01:intron 40.0%
AAGTAGGACATTAGATGGAG+AGG - Chr6:43590786-43590805 MsG0680032519.01.T01:intron 40.0%
AAGTCCCAACCCAAAAAAGT+AGG - Chr6:43590770-43590789 MsG0680032519.01.T01:intron 40.0%
AATTAGACGTGCAATCCCTT+CGG + Chr6:43591159-43591178 None:intergenic 40.0%
ACACCAAGGAAACAGAATGA+GGG + Chr6:43592188-43592207 None:intergenic 40.0%
AGAAGAAGAGAAGGGTTTGT+AGG + Chr6:43589181-43589200 None:intergenic 40.0%
AGCCAACATGATTGGTGAAA+CGG - Chr6:43591417-43591436 MsG0680032519.01.T01:intron 40.0%
AGTGTATTCTGTGTTGAGTG+GGG - Chr6:43591605-43591624 MsG0680032519.01.T01:intron 40.0%
ATCCACACACCGAAAAAATG+GGG + Chr6:43591805-43591824 None:intergenic 40.0%
ATGAAACACAGTGACCAAAG+GGG + Chr6:43593222-43593241 None:intergenic 40.0%
ATTTCGGTAGCCAACATGAT+TGG - Chr6:43591409-43591428 MsG0680032519.01.T01:intron 40.0%
CAAGTTCATGTCCAGCATTT+GGG - Chr6:43590959-43590978 MsG0680032519.01.T01:intron 40.0%
CAATCTTAGCGCCTAAACAA+AGG + Chr6:43592297-43592316 None:intergenic 40.0%
CAATGAAACACAGTGACCAA+AGG + Chr6:43593224-43593243 None:intergenic 40.0%
CGCTCATCTATCAGTATTCA+TGG - Chr6:43590886-43590905 MsG0680032519.01.T01:intron 40.0%
CTATACGATGTAACACACCA+AGG + Chr6:43592202-43592221 None:intergenic 40.0%
GCAGCGTAAGTTTATTGAGA+AGG - Chr6:43592384-43592403 MsG0680032519.01.T01:intron 40.0%
GCTAAGCGAAAAAAACAGCT+AGG + Chr6:43592484-43592503 None:intergenic 40.0%
GCTTCTTCTTCATGTAGTTC+CGG - Chr6:43592103-43592122 MsG0680032519.01.T01:intron 40.0%
GGAAACAATGAGTGTATGCT+TGG - Chr6:43589646-43589665 MsG0680032519.01.T01:CDS 40.0%
GGCTATGTAATCGAGCATTT+GGG - Chr6:43593286-43593305 MsG0680032519.01.T01:five_prime_UTR 40.0%
GGTAAAGAACTCACAGAACA+CGG + Chr6:43589429-43589448 None:intergenic 40.0%
GTGAAGGATTCCAGATTGTA+AGG - Chr6:43592417-43592436 MsG0680032519.01.T01:intron 40.0%
TAACAGCACCAAAACTACAC+AGG + Chr6:43592078-43592097 None:intergenic 40.0%
TAGCGACTGTCCTTCAAATT+GGG - Chr6:43591847-43591866 MsG0680032519.01.T01:intron 40.0%
TCAAGTTCATGTCCAGCATT+TGG - Chr6:43590958-43590977 MsG0680032519.01.T01:intron 40.0%
TCACGTTTCTTGGAGATAAG+TGG + Chr6:43590707-43590726 None:intergenic 40.0%
TGGCTATGTAATCGAGCATT+TGG - Chr6:43593285-43593304 MsG0680032519.01.T01:five_prime_UTR 40.0%
TGTCAAATGAGGACTTCACA+GGG + Chr6:43593454-43593473 None:intergenic 40.0%
TTGCTTGTAGGACCAACAAT+CGG - Chr6:43592984-43593003 MsG0680032519.01.T01:intron 40.0%
TTGTCAAATGAGGACTTCAC+AGG + Chr6:43593455-43593474 None:intergenic 40.0%
! AGGAGCATATTCCAGGTATA+CGG - Chr6:43593311-43593330 MsG0680032519.01.T01:five_prime_UTR 40.0%
! CGGTTGCAAAGTTGACTTTA+TGG + Chr6:43589409-43589428 None:intergenic 40.0%
! CTTTGTTTAGGCGCTAAGAT+TGG - Chr6:43592295-43592314 MsG0680032519.01.T01:intron 40.0%
! GAAAACGGGTTTGTCAAATG+AGG + Chr6:43593465-43593484 None:intergenic 40.0%
! GTTCTGTTCCTGTGTAGTTT+TGG - Chr6:43592067-43592086 MsG0680032519.01.T01:intron 40.0%
! TCGCAAGTCTTGTAACTTTG+CGG - Chr6:43592621-43592640 MsG0680032519.01.T01:intron 40.0%
! TGAGTGAACTCAAGTTTGCT+TGG + Chr6:43590585-43590604 None:intergenic 40.0%
! TGGTGCATGTTGCTATCTTT+TGG - Chr6:43592577-43592596 MsG0680032519.01.T01:intron 40.0%
! TGGTTGTTGATGGAAGATGA+TGG + Chr6:43589251-43589270 None:intergenic 40.0%
!! GTGTTCGTTTTTCCCAATGA+TGG - Chr6:43593193-43593212 MsG0680032519.01.T01:intron 40.0%
!! TTCGTCATGGCGTAGTTTTA+GGG - Chr6:43591508-43591527 MsG0680032519.01.T01:intron 40.0%
!!! CAGCTCCTTATTTTGGCATA+AGG - Chr6:43593504-43593523 MsG0680032519.01.T01:five_prime_UTR 40.0%
!!! TTTGCTTTTTTCCTTGCGCA+TGG - Chr6:43592339-43592358 MsG0680032519.01.T01:intron 40.0%
AAGAAAAGGAGGGAAGAGTG+TGG + Chr6:43589271-43589290 None:intergenic 45.0%
AATACCTCGTTCCGTATACC+TGG + Chr6:43593325-43593344 None:intergenic 45.0%
ACATGATTGGTGAAACGGAC+AGG - Chr6:43591422-43591441 MsG0680032519.01.T01:intron 45.0%
ACTGGTGGCTGAAGAAGAAA+AGG + Chr6:43589285-43589304 None:intergenic 45.0%
AGATGAGCGAGCCGTTAAAA+GGG + Chr6:43590875-43590894 None:intergenic 45.0%
AGCGACTGTCCTTCAAATTG+GGG - Chr6:43591848-43591867 MsG0680032519.01.T01:intron 45.0%
AGCTTTCATTCTATCGGCAC+TGG - Chr6:43590832-43590851 MsG0680032519.01.T01:intron 45.0%
ATTACTTTCCCTGCTTCCGA+AGG - Chr6:43591140-43591159 MsG0680032519.01.T01:intron 45.0%
CACACCAAGGAAACAGAATG+AGG + Chr6:43592189-43592208 None:intergenic 45.0%
CCTCGTGAGCTTAACTCATA+AGG + Chr6:43590749-43590768 None:intergenic 45.0%
CCTTATGAGTTAAGCTCACG+AGG - Chr6:43590746-43590765 MsG0680032519.01.T01:intron 45.0%
CGAACACAGACACAAAAGAG+AGG + Chr6:43589459-43589478 None:intergenic 45.0%
GAAGGGTTTGTAGGAGAAGA+AGG + Chr6:43589172-43589191 None:intergenic 45.0%
GTAGCGACTGTCCTTCAAAT+TGG - Chr6:43591846-43591865 MsG0680032519.01.T01:intron 45.0%
GTCCGTTTCACCAATCATGT+TGG + Chr6:43591422-43591441 None:intergenic 45.0%
GTGGCTGAAGAAGAAAAGGA+GGG + Chr6:43589281-43589300 None:intergenic 45.0%
TAGATGAGCGAGCCGTTAAA+AGG + Chr6:43590876-43590895 None:intergenic 45.0%
TAGCTCGCATCCTTACAATC+TGG + Chr6:43592430-43592449 None:intergenic 45.0%
TATTCCAGGTATACGGAACG+AGG - Chr6:43593318-43593337 MsG0680032519.01.T01:five_prime_UTR 45.0%
TATTCTGTGTTGAGTGGGGA+TGG - Chr6:43591609-43591628 MsG0680032519.01.T01:intron 45.0%
TGTAATCGAGCATTTGGGAG+AGG - Chr6:43593291-43593310 MsG0680032519.01.T01:five_prime_UTR 45.0%
TTACTTTCCCTGCTTCCGAA+GGG - Chr6:43591141-43591160 MsG0680032519.01.T01:intron 45.0%
TTGAGTCGGACTGCTTCTAA+TGG - Chr6:43592127-43592146 MsG0680032519.01.T01:intron 45.0%
! AGTTTTCTTGTCGGCAGTGT+TGG - Chr6:43592220-43592239 MsG0680032519.01.T01:intron 45.0%
! CATGTAGTTCCGGTTTGAGT+CGG - Chr6:43592113-43592132 MsG0680032519.01.T01:intron 45.0%
! GAAAGTGTGAGAGGGTTTGA+AGG + Chr6:43589225-43589244 None:intergenic 45.0%
! GACTTGGTAGAAGTTCGTCA+TGG - Chr6:43591495-43591514 MsG0680032519.01.T01:intron 45.0%
! GATGGAGAGAAAGTGTGAGA+GGG + Chr6:43589233-43589252 None:intergenic 45.0%
! GCTTCTAATGGTGAGAGTGT+CGG - Chr6:43592139-43592158 MsG0680032519.01.T01:intron 45.0%
! GTTCGTCATGGCGTAGTTTT+AGG - Chr6:43591507-43591526 MsG0680032519.01.T01:intron 45.0%
! TAGTGTAGTGGAGTTGGAGA+AGG - Chr6:43591451-43591470 MsG0680032519.01.T01:intron 45.0%
! TCTTTTTGTGTAGGTGGGTG+TGG - Chr6:43591742-43591761 MsG0680032519.01.T01:intron 45.0%
! TGATGGAGAGAAAGTGTGAG+AGG + Chr6:43589234-43589253 None:intergenic 45.0%
! TTTTCTTCTTCAGCCACCAG+TGG - Chr6:43589284-43589303 MsG0680032519.01.T01:intron 45.0%
!! CTTCTAATGGTGAGAGTGTC+GGG - Chr6:43592140-43592159 MsG0680032519.01.T01:intron 45.0%
AAAGGTTCCCCCTCTGACAA+CGG + Chr6:43591343-43591362 None:intergenic 50.0%
AACCAAGCACAAGGCATGCA+CGG + Chr6:43592677-43592696 None:intergenic 50.0%
ACCAAAGGGGCTTCCATCAT+TGG + Chr6:43593209-43593228 None:intergenic 50.0%
ACGTCGCCCTCAATGACAAA+TGG + Chr6:43593017-43593036 None:intergenic 50.0%
AGAAGCAGTCCGACTCAAAC+CGG + Chr6:43592125-43592144 None:intergenic 50.0%
AGGACTTCACAGGGTATAGC+TGG + Chr6:43593445-43593464 None:intergenic 50.0%
ATAGGCTGTTGCAACGACCA+GGG - Chr6:43591048-43591067 MsG0680032519.01.T01:intron 50.0%
CCAAAGGGGCTTCCATCATT+GGG + Chr6:43593208-43593227 None:intergenic 50.0%
GCTCCCTCATTCTGTTTCCT+TGG - Chr6:43592182-43592201 MsG0680032519.01.T01:intron 50.0%
GGTGGCTGAAGAAGAAAAGG+AGG + Chr6:43589282-43589301 None:intergenic 50.0%
TATAGGCTGTTGCAACGACC+AGG - Chr6:43591047-43591066 MsG0680032519.01.T01:intron 50.0%
TCCAACTCCACTACACTACC+TGG + Chr6:43591449-43591468 None:intergenic 50.0%
TCCATTGCTGAAGACAGCTG+AGG - Chr6:43593246-43593265 MsG0680032519.01.T01:intron 50.0%
TCCTCAGCTGTCTTCAGCAA+TGG + Chr6:43593250-43593269 None:intergenic 50.0%
TGATGGGATCCGTTGTCAGA+GGG - Chr6:43591331-43591350 MsG0680032519.01.T01:intron 50.0%
TGCTCAGAGCCCCAATTTGA+AGG + Chr6:43591860-43591879 None:intergenic 50.0%
TTGATGGGATCCGTTGTCAG+AGG - Chr6:43591330-43591349 MsG0680032519.01.T01:intron 50.0%
! GGGAAGAGTGTGGTTGTTGA+TGG + Chr6:43589261-43589280 None:intergenic 50.0%
! TCCAGGTAGTGTAGTGGAGT+TGG - Chr6:43591445-43591464 MsG0680032519.01.T01:intron 50.0%
! TTGGGAGAGGAGCATATTCC+AGG - Chr6:43593304-43593323 MsG0680032519.01.T01:five_prime_UTR 50.0%
!! CGTGCTGCAGCTCCTTATTT+TGG - Chr6:43593497-43593516 MsG0680032519.01.T01:five_prime_UTR 50.0%
!! TAATGGTGAGAGTGTCGGGT+GGG - Chr6:43592144-43592163 MsG0680032519.01.T01:intron 50.0%
!!! TGCCCCATTTTTTCGGTGTG+TGG - Chr6:43591800-43591819 MsG0680032519.01.T01:intron 50.0%
ACCAAGCACAAGGCATGCAC+GGG + Chr6:43592676-43592695 None:intergenic 55.0%
ATGGGATCCGTTGTCAGAGG+GGG - Chr6:43591333-43591352 MsG0680032519.01.T01:intron 55.0%
CAGCACGTGCACACTCACAT+GGG - Chr6:43589307-43589326 MsG0680032519.01.T01:intron 55.0%
GAACTCTCAGAGAAGCCTGC+AGG - Chr6:43592913-43592932 MsG0680032519.01.T01:intron 55.0%
GACAGGTCCAGGTAGTGTAG+TGG - Chr6:43591439-43591458 MsG0680032519.01.T01:intron 55.0%
GAGGAAAAAACCAGCGCGCT+TGG - Chr6:43593265-43593284 MsG0680032519.01.T01:five_prime_UTR 55.0%
GATGGGATCCGTTGTCAGAG+GGG - Chr6:43591332-43591351 MsG0680032519.01.T01:intron 55.0%
GATTACATAGCCAAGCGCGC+TGG + Chr6:43593278-43593297 None:intergenic 55.0%
GTCATTGAGGGCGACGTTGT+TGG - Chr6:43593020-43593039 MsG0680032519.01.T01:intron 55.0%
GTGCAATCCCTTCGGAAGCA+GGG + Chr6:43591151-43591170 None:intergenic 55.0%
TTGGTGAAACGGACAGGTCC+AGG - Chr6:43591428-43591447 MsG0680032519.01.T01:intron 55.0%
! AGAAGGGTTGGCAGCAGTGA+AGG - Chr6:43592401-43592420 MsG0680032519.01.T01:intron 55.0%
! CCCAATGATGGAAGCCCCTT+TGG - Chr6:43593205-43593224 MsG0680032519.01.T01:intron 55.0%
!! ATGGCAGTGAGGTGGTGGAT+TGG - Chr6:43591628-43591647 MsG0680032519.01.T01:intron 55.0%
!! CTAATGGTGAGAGTGTCGGG+TGG - Chr6:43592143-43592162 MsG0680032519.01.T01:intron 55.0%
!! TGAGGTGGTGGATTGGGCTT+CGG - Chr6:43591635-43591654 MsG0680032519.01.T01:intron 55.0%
!! TGGCAGTGAGGTGGTGGATT+GGG - Chr6:43591629-43591648 MsG0680032519.01.T01:intron 55.0%
CGACTCGACAACGTTTGCCC+TGG + Chr6:43591068-43591087 None:intergenic 60.0%
CGTGCAATCCCTTCGGAAGC+AGG + Chr6:43591152-43591171 None:intergenic 60.0%
GCAGCACGTGCACACTCACA+TGG - Chr6:43589306-43589325 MsG0680032519.01.T01:intron 60.0%
GTTGAGTGGGGATGGCAGTG+AGG - Chr6:43591617-43591636 MsG0680032519.01.T01:intron 60.0%
TCCCGTGCATGCCTTGTGCT+TGG - Chr6:43592672-43592691 MsG0680032519.01.T01:intron 60.0%
GAGTGGGGATGGCAGTGAGG+TGG - Chr6:43591620-43591639 MsG0680032519.01.T01:intron 65.0%
GAGTGTGCACGTGCTGCCAC+TGG + Chr6:43589303-43589322 None:intergenic 65.0%
TGGGGATGGCAGTGAGGTGG+TGG - Chr6:43591623-43591642 MsG0680032519.01.T01:intron 65.0%
TGTGCACGTGCTGCCACTGG+TGG + Chr6:43589300-43589319 None:intergenic 65.0%
!! GGTGAGAGTGTCGGGTGGGT+TGG - Chr6:43592148-43592167 MsG0680032519.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 43589131 43593553 43589131 ID=MsG0680032519.01;Name=MsG0680032519.01
Chr6 mRNA 43589131 43593553 43589131 ID=MsG0680032519.01.T01;Parent=MsG0680032519.01;Name=MsG0680032519.01.T01;_AED=0.50;_eAED=0.58;_QI=312|0.5|0.4|1|0.5|0.6|5|0|146
Chr6 exon 43593250 43593553 43593250 ID=MsG0680032519.01.T01:exon:11676;Parent=MsG0680032519.01.T01
Chr6 exon 43590042 43590109 43590042 ID=MsG0680032519.01.T01:exon:11677;Parent=MsG0680032519.01.T01
Chr6 exon 43589627 43589721 43589627 ID=MsG0680032519.01.T01:exon:11678;Parent=MsG0680032519.01.T01
Chr6 exon 43589345 43589522 43589345 ID=MsG0680032519.01.T01:exon:11679;Parent=MsG0680032519.01.T01
Chr6 exon 43589131 43589238 43589131 ID=MsG0680032519.01.T01:exon:11680;Parent=MsG0680032519.01.T01
Chr6 five_prime_UTR 43593250 43593553 43593250 ID=MsG0680032519.01.T01:five_prime_utr;Parent=MsG0680032519.01.T01
Chr6 five_prime_UTR 43590102 43590109 43590102 ID=MsG0680032519.01.T01:five_prime_utr;Parent=MsG0680032519.01.T01
Chr6 CDS 43590042 43590101 43590042 ID=MsG0680032519.01.T01:cds;Parent=MsG0680032519.01.T01
Chr6 CDS 43589627 43589721 43589627 ID=MsG0680032519.01.T01:cds;Parent=MsG0680032519.01.T01
Chr6 CDS 43589345 43589522 43589345 ID=MsG0680032519.01.T01:cds;Parent=MsG0680032519.01.T01
Chr6 CDS 43589131 43589238 43589131 ID=MsG0680032519.01.T01:cds;Parent=MsG0680032519.01.T01
Gene Sequence

>MsG0680032519.01.T01

ATGTTGCTATCTTTTGGCACGTTTGTGAACTCTTTTTTTCGCAAGTCTTGTAACTTTGCGATTGTCTTGTTGATTTCGAAATTGCTTGTAGGACCAACAATCGGAATTCTTCCATTTGTCATTGAGGGCGACGTTGTTGGATTCATTCACAATCGGTTTGTTGAGCATTTAAGAGGCTTTAATGATGTGTTCGTTTTTCCCAATGATGGAAGCCCCTTTGGTCACTGTGTTTCATTGCATCCATTGCTGAAGACAGCTGAGGAAAAAACCAGCGCGCTTGGCTATGTAATCGAGCATTTGGGAGAGGAGCATATTCCAGGTATACGGAACGAGCTATACCCTGTGAAGTCCTCATTTGACAAACCCGTTTTCTTTTCATTAGAACGTGCTGCAGCTCCTTATTTTGGCATAAGGTACAAGAAAGAATACGCTGATACTTAA
Protein sequence

>MsG0680032519.01.T01

MLLSFGTFVNSFFRKSCNFAIVLLISKLLVGPTIGILPFVIEGDVVGFIHNRFVEHLRGFNDVFVFPNDGSPFGHCVSLHPLLKTAEEKTSALGYVIEHLGEEHIPGIRNELYPVKSSFDKPVFFSLERAAAPYFGIRYKKEYADT*