AlfalfaGEDB | MsG0680032854.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032854.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032854.01.T01	XP_024626737.2	71.429	203	29	1	3	176	45	247	3.45E-97	291

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032854.01.T01	A0A396GZG8	71.429	203	29	1	3	176	63	265	5.58e-97	290

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0680032854.01	MsG0780041116.01	PPI
MsG0780040274.01	MsG0680032854.01	PPI
MsG0680032854.01	MsG0280010449.01	PPI
MsG0280009322.01	MsG0680032854.01	PPI
MsG0680032854.01	MsG0780041116.01	PPI
MsG0680032854.01	MsG0380013578.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032854.01.T01	MTR_5g091440	56.489	131	23	2	46	175	2	99	4.23e-39	130
MsG0680032854.01.T01	MTR_8g088220	83.333	66	11	0	56	121	12	77	4.20e-34	115
MsG0680032854.01.T01	MTR_4g010930	89.655	58	6	0	79	136	1	58	1.58e-30	109
MsG0680032854.01.T01	MTR_1g081930	45.600	125	39	1	35	130	6	130	1.76e-26	97.8
MsG0680032854.01.T01	MTR_7g063280	48.193	83	43	0	56	138	90	172	1.87e-23	91.7
MsG0680032854.01.T01	MTR_4g023580	42.574	101	37	3	2	86	72	167	1.18e-18	79.0
MsG0680032854.01.T01	MTR_5g031620	46.988	83	44	0	93	175	139	221	2.22e-17	76.6
MsG0680032854.01.T01	MTR_7g053400	31.056	161	60	4	2	149	130	252	4.35e-17	76.6
MsG0680032854.01.T01	MTR_6g016265	44.928	69	38	0	56	124	57	125	9.84e-17	72.8
MsG0680032854.01.T01	MTR_1g045560	39.000	100	37	2	56	151	13	92	1.05e-16	71.6
MsG0680032854.01.T01	MTR_6g034915	38.462	130	60	3	47	174	63	174	1.08e-16	73.9
MsG0680032854.01.T01	MTR_7g072030	46.575	73	39	0	39	111	50	122	2.92e-16	71.2
MsG0680032854.01.T01	MTR_7g077900	54.688	64	29	0	112	175	64	127	5.97e-16	71.2
MsG0680032854.01.T01	MTR_5g069700	49.254	67	34	0	59	125	2	68	1.60e-15	68.2
MsG0680032854.01.T01	MTR_3g116210	30.822	146	70	2	6	122	38	181	2.48e-15	70.5
MsG0680032854.01.T01	MTR_4g036855	35.897	117	47	1	59	175	2	90	7.95e-15	66.6
MsG0680032854.01.T01	MTR_8g038340	42.045	88	22	1	37	95	159	246	8.73e-15	70.1
MsG0680032854.01.T01	MTR_7g082560	35.714	112	41	1	58	169	26	106	1.48e-14	66.6
MsG0680032854.01.T01	MTR_1g057740	31.677	161	85	3	16	176	6	141	6.05e-14	65.9
MsG0680032854.01.T01	MTR_5g075610	36.634	101	61	2	47	146	3	101	6.57e-14	64.7
MsG0680032854.01.T01	MTR_8g028785	31.138	167	90	3	2	145	201	365	9.74e-14	68.2
MsG0680032854.01.T01	MTR_5g049680	55.102	49	22	0	56	104	49	97	1.01e-13	64.3
MsG0680032854.01.T01	MTR_1g074090	32.110	109	45	1	25	104	39	147	1.39e-13	65.1
MsG0680032854.01.T01	MTR_2g027020	35.000	120	74	1	56	175	46	161	1.40e-13	65.9
MsG0680032854.01.T01	MTR_4g064590	32.479	117	73	2	61	175	49	161	1.88e-13	65.1
MsG0680032854.01.T01	MTR_5g083200	30.822	146	62	2	6	122	47	182	4.07e-13	64.7
MsG0680032854.01.T01	MTR_0002s1220	30.822	146	62	2	6	122	47	182	4.07e-13	64.7
MsG0680032854.01.T01	MTR_3g090920	31.707	123	68	3	54	176	3	109	8.10e-13	62.0
MsG0680032854.01.T01	MTR_3g111390	30.822	146	62	2	6	122	47	182	8.29e-13	63.9
MsG0680032854.01.T01	MTR_1g070820	30.822	146	62	2	6	122	47	182	8.29e-13	63.9
MsG0680032854.01.T01	MTR_4g008270	41.667	72	41	1	47	117	18	89	1.96e-12	60.5
MsG0680032854.01.T01	MTR_0015s0120	51.786	56	27	0	56	111	311	366	2.28e-12	64.3
MsG0680032854.01.T01	MTR_1g045340	41.026	78	45	1	46	122	91	168	5.84e-12	61.6
MsG0680032854.01.T01	MTR_8g022750	32.143	112	64	1	36	135	174	285	1.09e-11	62.0
MsG0680032854.01.T01	MTR_1g088650	39.535	86	52	0	56	141	104	189	1.32e-11	60.5
MsG0680032854.01.T01	MTR_8g059135	34.375	96	56	2	43	131	26	121	1.46e-11	59.3
MsG0680032854.01.T01	MTR_7g013520	33.945	109	47	1	67	175	99	182	2.82e-11	59.7
MsG0680032854.01.T01	MTR_1g017820	39.683	63	38	0	46	108	8	70	2.97e-11	57.0
MsG0680032854.01.T01	MTR_6g016135	58.696	46	19	0	56	101	397	442	6.29e-11	60.5
MsG0680032854.01.T01	MTR_6g045427	33.721	86	56	1	46	130	152	237	7.07e-11	59.3
MsG0680032854.01.T01	MTR_1g103580	30.657	137	72	3	39	175	132	245	7.94e-11	59.7
MsG0680032854.01.T01	MTR_5g083510	36.842	76	48	0	100	175	16	91	9.38e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGTTTCCATATTCATATT+AGG	0.250520	6:-51189692	MsG0680032854.01.T01:CDS
AACATTACATTTCAATTTGC+CGG	0.276899	6:+51190091	None:intergenic
TTGGGTTCGCGATCTGCATC+TGG	0.289846	6:-51189916	MsG0680032854.01.T01:CDS
ATGGAATCCTAATATGAATA+TGG	0.299221	6:+51189685	None:intergenic
CCTCTGATGTTAGGTTTATT+CGG	0.300562	6:-51189767	MsG0680032854.01.T01:CDS
AAGACTATCTACCACAGTTT+TGG	0.314118	6:+51189869	None:intergenic
ATTTAGTAACCTCTGATGTT+AGG	0.328034	6:-51189776	MsG0680032854.01.T01:CDS
TTTATTATCAGCTATGTATT+GGG	0.337276	6:-51189934	MsG0680032854.01.T01:CDS
TAATCCACTTCAAGTCATTC+GGG	0.364086	6:+51190123	None:intergenic
TTAATCCACTTCAAGTCATT+CGG	0.365756	6:+51190122	None:intergenic
GTTTATTATCAGCTATGTAT+TGG	0.370009	6:-51189935	MsG0680032854.01.T01:CDS
TTACTAAATTAGAAGCTAAT+AGG	0.370318	6:+51189790	None:intergenic
CATTTCAATTTGCCGGGACT+TGG	0.372745	6:+51190098	None:intergenic
GTGTATTTGAGTGAGGCTTT+AGG	0.402801	6:-51189957	MsG0680032854.01.T01:CDS
CAGGATCTCTTCTTACTAGT+TGG	0.412858	6:-51190193	MsG0680032854.01.T01:CDS
ATAATAGTAGAGATACTTGA+TGG	0.428257	6:+51189666	None:intergenic
CGCTCATAACTTTGCTAGAG+TGG	0.455273	6:-51189727	MsG0680032854.01.T01:CDS
TTCAAGTCATTCGGGGTAGC+GGG	0.464805	6:+51190131	None:intergenic
CAAGTTATTTGTGCTCGTGC+AGG	0.477021	6:-51190212	MsG0680032854.01.T01:CDS
TTCGGGGTAGCGGGATGCTG+AGG	0.486358	6:+51190140	None:intergenic
AGCATTGAAACAATATGGTA+TGG	0.501977	6:-51190256	None:intergenic
CTTCAAGTCATTCGGGGTAG+CGG	0.506155	6:+51190130	None:intergenic
CGATCTGCATCTGGGTATTG+TGG	0.516629	6:-51189907	MsG0680032854.01.T01:CDS
ACATTACATTTCAATTTGCC+GGG	0.518563	6:+51190092	None:intergenic
CTACCCCGAATGACTTGAAG+TGG	0.520477	6:-51190127	MsG0680032854.01.T01:CDS
CCTTGATGTGTATTTGAGTG+AGG	0.520805	6:-51189964	MsG0680032854.01.T01:CDS
ATGTGTTAGCCAAGACTGAA+TGG	0.525614	6:-51189998	MsG0680032854.01.T01:intron
AGGAGTCATCCATTCAGTCT+TGG	0.552597	6:+51189989	None:intergenic
TCGGGGTAGCGGGATGCTGA+GGG	0.558111	6:+51190141	None:intergenic
TGGGTTCGCGATCTGCATCT+GGG	0.563552	6:-51189915	MsG0680032854.01.T01:CDS
CCTCACTCAAATACACATCA+AGG	0.571445	6:+51189964	None:intergenic
AAGTGGATTAAACCAAGTCC+CGG	0.576242	6:-51190110	MsG0680032854.01.T01:CDS
AGTCTTTATGATAGTAAGAG+TGG	0.591171	6:-51189852	MsG0680032854.01.T01:CDS
AAAGTTATGAGCGACTTCGT+TGG	0.600023	6:+51189737	None:intergenic
CCGAATAAACCTAACATCAG+AGG	0.603230	6:+51189767	None:intergenic
CTCAAATACACATCAAGGAG+AGG	0.629689	6:+51189969	None:intergenic
TGAGCTTGATTCCAAAACTG+TGG	0.664726	6:-51189880	MsG0680032854.01.T01:CDS
AATCCACTTCAAGTCATTCG+GGG	0.690299	6:+51190124	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTACTAAATTAGAAGCTAAT+AGG	+	Chr6:51190102-51190121	None:intergenic	20.0%
!!	TTTATTATCAGCTATGTATT+GGG	-	Chr6:51189955-51189974	MsG0680032854.01.T01:CDS	20.0%
!	AACATTACATTTCAATTTGC+CGG	+	Chr6:51189801-51189820	None:intergenic	25.0%
!	ATAATAGTAGAGATACTTGA+TGG	+	Chr6:51190226-51190245	None:intergenic	25.0%
!	ATGGAATCCTAATATGAATA+TGG	+	Chr6:51190207-51190226	None:intergenic	25.0%
!	ATTGAAATGTAATGTTGATG+CGG	-	Chr6:51189805-51189824	MsG0680032854.01.T01:CDS	25.0%
!	CTAGTTTCCATATTCATATT+AGG	-	Chr6:51190197-51190216	MsG0680032854.01.T01:CDS	25.0%
!	GTTTATTATCAGCTATGTAT+TGG	-	Chr6:51189954-51189973	MsG0680032854.01.T01:CDS	25.0%
	ACATTACATTTCAATTTGCC+GGG	+	Chr6:51189800-51189819	None:intergenic	30.0%
	AGTCTTTATGATAGTAAGAG+TGG	-	Chr6:51190037-51190056	MsG0680032854.01.T01:intron	30.0%
	ATTTAGTAACCTCTGATGTT+AGG	-	Chr6:51190113-51190132	MsG0680032854.01.T01:CDS	30.0%
	TTAATCCACTTCAAGTCATT+CGG	+	Chr6:51189770-51189789	None:intergenic	30.0%
	CCTCTGATGTTAGGTTTATT+CGG	-	Chr6:51190122-51190141	MsG0680032854.01.T01:CDS	35.0%
	TAATCCACTTCAAGTCATTC+GGG	+	Chr6:51189769-51189788	None:intergenic	35.0%
!	AAGACTATCTACCACAGTTT+TGG	+	Chr6:51190023-51190042	None:intergenic	35.0%
!!!	GTGTGATTTTGAGATGATGA+AGG	-	Chr6:51189863-51189882	MsG0680032854.01.T01:CDS	35.0%
	AAAGTTATGAGCGACTTCGT+TGG	+	Chr6:51190155-51190174	None:intergenic	40.0%
	AATCCACTTCAAGTCATTCG+GGG	+	Chr6:51189768-51189787	None:intergenic	40.0%
	ATGTGTTAGCCAAGACTGAA+TGG	-	Chr6:51189891-51189910	MsG0680032854.01.T01:CDS	40.0%
	CAGGATCTCTTCTTACTAGT+TGG	-	Chr6:51189696-51189715	MsG0680032854.01.T01:CDS	40.0%
	CCGAATAAACCTAACATCAG+AGG	+	Chr6:51190125-51190144	None:intergenic	40.0%
	CCTCACTCAAATACACATCA+AGG	+	Chr6:51189928-51189947	None:intergenic	40.0%
	CCTTGATGTGTATTTGAGTG+AGG	-	Chr6:51189925-51189944	MsG0680032854.01.T01:CDS	40.0%
	CTCAAATACACATCAAGGAG+AGG	+	Chr6:51189923-51189942	None:intergenic	40.0%
	GTGTATTTGAGTGAGGCTTT+AGG	-	Chr6:51189932-51189951	MsG0680032854.01.T01:CDS	40.0%
	TCGATTCCGAACTTGTGAAA+AGG	+	Chr6:51189831-51189850	None:intergenic	40.0%
	TGAGCTTGATTCCAAAACTG+TGG	-	Chr6:51190009-51190028	MsG0680032854.01.T01:intron	40.0%
!!	AAGTGGATTAAACCAAGTCC+CGG	-	Chr6:51189779-51189798	MsG0680032854.01.T01:CDS	40.0%
	AGGAGTCATCCATTCAGTCT+TGG	+	Chr6:51189903-51189922	None:intergenic	45.0%
	CAAGTTATTTGTGCTCGTGC+AGG	-	Chr6:51189677-51189696	MsG0680032854.01.T01:CDS	45.0%
	CATTTCAATTTGCCGGGACT+TGG	+	Chr6:51189794-51189813	None:intergenic	45.0%
	CGCTCATAACTTTGCTAGAG+TGG	-	Chr6:51190162-51190181	MsG0680032854.01.T01:CDS	45.0%
	TCACAAGTTCGGAATCGAGT+TGG	-	Chr6:51189833-51189852	MsG0680032854.01.T01:CDS	45.0%
!	ATGCGGCCTTTTCACAAGTT+CGG	-	Chr6:51189822-51189841	MsG0680032854.01.T01:CDS	45.0%
	CTACCCCGAATGACTTGAAG+TGG	-	Chr6:51189762-51189781	MsG0680032854.01.T01:CDS	50.0%
	CTTCAAGTCATTCGGGGTAG+CGG	+	Chr6:51189762-51189781	None:intergenic	50.0%
	TTCAAGTCATTCGGGGTAGC+GGG	+	Chr6:51189761-51189780	None:intergenic	50.0%
!	CGATCTGCATCTGGGTATTG+TGG	-	Chr6:51189982-51190001	MsG0680032854.01.T01:CDS	50.0%
!!	GTTCGGAATCGAGTTGGCAT+TGG	-	Chr6:51189839-51189858	MsG0680032854.01.T01:CDS	50.0%
	TGGGTTCGCGATCTGCATCT+GGG	-	Chr6:51189974-51189993	MsG0680032854.01.T01:CDS	55.0%
	TTGGGTTCGCGATCTGCATC+TGG	-	Chr6:51189973-51189992	MsG0680032854.01.T01:CDS	55.0%
!!	TCGGGGTAGCGGGATGCTGA+GGG	+	Chr6:51189751-51189770	None:intergenic	65.0%
!!	TTCGGGGTAGCGGGATGCTG+AGG	+	Chr6:51189752-51189771	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	51189647	51190264	51189647	ID=MsG0680032854.01;Name=MsG0680032854.01
Chr6	mRNA	51189647	51190264	51189647	ID=MsG0680032854.01.T01;Parent=MsG0680032854.01;Name=MsG0680032854.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|2\|0\|176
Chr6	exon	51190099	51190264	51190099	ID=MsG0680032854.01.T01:exon:13612;Parent=MsG0680032854.01.T01
Chr6	exon	51189647	51190011	51189647	ID=MsG0680032854.01.T01:exon:13613;Parent=MsG0680032854.01.T01
Chr6	CDS	51190099	51190264	51190099	ID=MsG0680032854.01.T01:cds;Parent=MsG0680032854.01.T01
Chr6	CDS	51189647	51190011	51189647	ID=MsG0680032854.01.T01:cds;Parent=MsG0680032854.01.T01

Gene Sequence

>MsG0680032854.01.T01

ATGGTATGGAACAATGTTACTGAAACAACTCAAGTTATTTGTGCTCGTGCAGGATCTCTTCTTACTAGTTGGAAGAATTCTCAGACTATTCGTCATCATATCCCTCAGCATCCCGCTACCCCGAATGACTTGAAGTGGATTAAACCAAGTCCCGGCAAATTGAAATCCAAGACTGAATGGATGACTCCTCTCCTTGATGTGTATTTGAGTGAGGCTTTAGGTTTATTATCAGCTATGTATTGGGTTCGCGATCTGCATCTGGGTATTGTGGACTTTGAGCTTGATTCCAAAACTGTGGTAGATAGTCTTTATGATAGTAAGAGTGGCGTCTCTAATTTTAGCGCAGTAATCAATGACTGTAGACGCCTATTAGCTTCTAATTTAGTAACCTCTGATGTTAGGTTTATTCGGAGACAAGCCAACGAAGTCGCTCATAACTTTGCTAGAGTGGCTTTGCATCATGCTAGTTTCCATATTCATATTAGGATTCCATCAAGTATCTCTACTATTATTATGAATGAAATGCAATAA

Protein sequence

>MsG0680032854.01.T01

MVWNNVTETTQVICARAGSLLTSWKNSQTIRHHIPQHPATPNDLKWIKPSPGKLKSKTEWMTPLLDVYLSEALGLLSAMYWVRDLHLGIVDFELDSKTVVDSLYDSKSGVSNFSAVINDCRRLLASNLVTSDVRFIRRQANEVAHNFARVALHHASFHIHIRIPSSISTIIMNEMQ*