Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032938.01.T01 | XP_004488690.1 | 46.903 | 226 | 115 | 3 | 1 | 226 | 1 | 221 | 2.56E-62 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032938.01.T01 | P08688 | 37.719 | 228 | 135 | 5 | 6 | 229 | 7 | 231 | 9.03E-44 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032938.01.T01 | A0A1S2XHS6 | 46.903 | 226 | 115 | 3 | 1 | 226 | 1 | 221 | 1.22e-62 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032938.01 | MsG0680032942.01 | 0.807241 | 5.564530e-50 | 3.083577e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032938.01.T01 | MTR_1g066530 | 44.889 | 225 | 119 | 3 | 1 | 225 | 1 | 220 | 2.16e-60 | 189 |
| MsG0680032938.01.T01 | MTR_6g048910 | 46.018 | 226 | 112 | 4 | 4 | 225 | 3 | 222 | 2.94e-57 | 181 |
| MsG0680032938.01.T01 | MTR_6g048620 | 44.493 | 227 | 120 | 5 | 3 | 225 | 2 | 226 | 1.78e-55 | 177 |
| MsG0680032938.01.T01 | MTR_0695s0030 | 41.593 | 226 | 128 | 4 | 6 | 229 | 65 | 288 | 9.62e-55 | 177 |
| MsG0680032938.01.T01 | MTR_6g073540 | 44.053 | 227 | 119 | 3 | 1 | 221 | 1 | 225 | 1.64e-54 | 174 |
| MsG0680032938.01.T01 | MTR_8g038070 | 41.593 | 226 | 128 | 4 | 6 | 229 | 219 | 442 | 3.75e-54 | 180 |
| MsG0680032938.01.T01 | MTR_6g045077 | 41.518 | 224 | 123 | 3 | 4 | 225 | 3 | 220 | 5.63e-53 | 170 |
| MsG0680032938.01.T01 | MTR_0807s0020 | 42.667 | 225 | 116 | 5 | 1 | 222 | 1 | 215 | 1.23e-50 | 164 |
| MsG0680032938.01.T01 | MTR_8g038100 | 41.150 | 226 | 129 | 4 | 6 | 229 | 281 | 504 | 1.24e-50 | 172 |
| MsG0680032938.01.T01 | MTR_6g072470 | 46.766 | 201 | 99 | 3 | 1 | 195 | 1 | 199 | 3.37e-50 | 163 |
| MsG0680032938.01.T01 | MTR_6g072470 | 35.811 | 148 | 82 | 4 | 63 | 199 | 6 | 151 | 1.74e-18 | 80.9 |
| MsG0680032938.01.T01 | MTR_0216s0030 | 43.367 | 196 | 103 | 3 | 5 | 197 | 2 | 192 | 2.82e-49 | 160 |
| MsG0680032938.01.T01 | MTR_0057s0100 | 38.767 | 227 | 133 | 6 | 6 | 229 | 7 | 230 | 2.53e-46 | 154 |
| MsG0680032938.01.T01 | MTR_0057s0170 | 37.885 | 227 | 135 | 6 | 6 | 229 | 7 | 230 | 4.98e-45 | 150 |
| MsG0680032938.01.T01 | MTR_6g040290 | 37.118 | 229 | 137 | 6 | 6 | 229 | 7 | 233 | 6.66e-41 | 140 |
| MsG0680032938.01.T01 | MTR_7g103990 | 38.596 | 228 | 133 | 6 | 6 | 229 | 7 | 231 | 2.92e-38 | 133 |
| MsG0680032938.01.T01 | MTR_7g012000 | 40.394 | 203 | 90 | 7 | 6 | 206 | 7 | 180 | 1.45e-36 | 128 |
| MsG0680032938.01.T01 | MTR_6g048810 | 30.164 | 305 | 123 | 8 | 4 | 225 | 3 | 300 | 7.82e-30 | 112 |
| MsG0680032938.01.T01 | MTR_6g049040 | 45.185 | 135 | 59 | 2 | 1 | 135 | 1 | 120 | 4.05e-29 | 106 |
| MsG0680032938.01.T01 | MTR_6g049040 | 33.613 | 119 | 67 | 2 | 72 | 189 | 15 | 122 | 7.60e-12 | 61.2 |
| MsG0680032938.01.T01 | MTR_4g102470 | 33.838 | 198 | 97 | 6 | 6 | 191 | 7 | 182 | 2.55e-28 | 107 |
| MsG0680032938.01.T01 | MTR_4g102470 | 35.000 | 140 | 72 | 3 | 63 | 200 | 7 | 129 | 5.72e-15 | 71.6 |
| MsG0680032938.01.T01 | MTR_6g048880 | 37.569 | 181 | 102 | 5 | 50 | 225 | 4 | 178 | 5.21e-27 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATGTAACATTGATTTC+TGG | 0.249542 | 6:+53509270 | None:intergenic |
| GAAAAGATTACTCGTCGTTT+TGG | 0.341596 | 6:-53508854 | MsG0680032938.01.T01:CDS |
| ACGTGGAGAATGTTTGATTT+TGG | 0.345783 | 6:+53509060 | None:intergenic |
| CACTGTGAGCATCTTTAAAT+AGG | 0.360670 | 6:+53508915 | None:intergenic |
| CAATTAGGTACAGGGATAAT+TGG | 0.373920 | 6:-53508946 | MsG0680032938.01.T01:CDS |
| ATGGAATAGATGCTGCAATT+AGG | 0.395457 | 6:-53508961 | MsG0680032938.01.T01:CDS |
| CAAGGAAACATGTCAGCAAT+TGG | 0.395598 | 6:+53509007 | None:intergenic |
| GCAGGCCATTCTTCACTGAT+TGG | 0.398205 | 6:+53508674 | None:intergenic |
| TCAAACATTTCATGAATAGC+AGG | 0.452097 | 6:+53508656 | None:intergenic |
| ATATCTTTCTCCTCACCTGC+TGG | 0.456795 | 6:+53509214 | None:intergenic |
| AATGAGGCATTCATCTTCGC+TGG | 0.471668 | 6:-53509094 | MsG0680032938.01.T01:CDS |
| TGACATTGAAAATATTAATG+AGG | 0.484830 | 6:-53509110 | MsG0680032938.01.T01:CDS |
| CAATCTATTCCATGATCAAA+CGG | 0.490576 | 6:+53509139 | None:intergenic |
| TAGATGCTGCAATTAGGTAC+AGG | 0.491307 | 6:-53508955 | MsG0680032938.01.T01:CDS |
| ATACCATTGGTGCTGCATTC+CGG | 0.493051 | 6:-53509300 | MsG0680032938.01.T01:CDS |
| GAGGAGAAAGATATTATTAG+TGG | 0.500890 | 6:-53509205 | MsG0680032938.01.T01:CDS |
| AACATGTCAGCAATTGGAAT+AGG | 0.502888 | 6:+53509013 | None:intergenic |
| ATTGATTTCTGGTAGGTGAC+CGG | 0.509826 | 6:+53509281 | None:intergenic |
| GTGGGTAAACCGTTTGATCA+TGG | 0.512654 | 6:-53509148 | MsG0680032938.01.T01:CDS |
| TAAAACCAATCAGTGAAGAA+TGG | 0.516703 | 6:-53508679 | MsG0680032938.01.T01:CDS |
| GCATCTATTCCATGTGCAAA+CGG | 0.521449 | 6:+53508971 | None:intergenic |
| TATGCCTCAAACAGCCATCA+TGG | 0.543398 | 6:-53508725 | MsG0680032938.01.T01:CDS |
| GATGGGTTTCCTTGTCCCGT+GGG | 0.545017 | 6:-53509166 | MsG0680032938.01.T01:CDS |
| GTCACCATGATGGCTGTTTG+AGG | 0.545899 | 6:+53508721 | None:intergenic |
| AACGGTTTACCCACGGGACA+AGG | 0.547178 | 6:+53509157 | None:intergenic |
| AAAATGTCTCATCATACCAT+TGG | 0.554275 | 6:-53509313 | MsG0680032938.01.T01:CDS |
| TATGGTACAGTGTTTGAGAG+TGG | 0.558207 | 6:-53508824 | MsG0680032938.01.T01:CDS |
| TGATCAAACGGTTTACCCAC+GGG | 0.564926 | 6:+53509151 | None:intergenic |
| AGATTATGCTCCAGCAGGTG+AGG | 0.567623 | 6:-53509224 | MsG0680032938.01.T01:CDS |
| TGATGGGTTTCCTTGTCCCG+TGG | 0.567999 | 6:-53509167 | MsG0680032938.01.T01:CDS |
| AACCCATCAGTAATATCGCT+CGG | 0.571730 | 6:+53509181 | None:intergenic |
| GGGCCGAGCGATATTACTGA+TGG | 0.572454 | 6:-53509184 | MsG0680032938.01.T01:CDS |
| ATGTAACATTGATTTCTGGT+AGG | 0.581051 | 6:+53509274 | None:intergenic |
| AGGAGAAAGATATTATTAGT+GGG | 0.583787 | 6:-53509204 | MsG0680032938.01.T01:CDS |
| TGACCGGAATGCAGCACCAA+TGG | 0.584763 | 6:+53509297 | None:intergenic |
| GAGAAAACACCGTTTGCACA+TGG | 0.594864 | 6:-53508980 | MsG0680032938.01.T01:CDS |
| ATGATCAAACGGTTTACCCA+CGG | 0.596173 | 6:+53509150 | None:intergenic |
| AGTATCTCTGCGTCTGAACG+TGG | 0.609086 | 6:+53509043 | None:intergenic |
| GGCCGAGCGATATTACTGAT+GGG | 0.620145 | 6:-53509183 | MsG0680032938.01.T01:CDS |
| GTTTGAGAGTGGAATTGATG+CGG | 0.652712 | 6:-53508813 | MsG0680032938.01.T01:CDS |
| AGATGCTGCAATTAGGTACA+GGG | 0.654178 | 6:-53508954 | MsG0680032938.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAATATTTTCAATGTCAA+AGG | + | Chr6:53508842-53508861 | None:intergenic | 15.0% |
| !! | TGACATTGAAAATATTAATG+AGG | - | Chr6:53508843-53508862 | MsG0680032938.01.T01:CDS | 20.0% |
| ! | AGGAGAAAGATATTATTAGT+GGG | - | Chr6:53508749-53508768 | MsG0680032938.01.T01:CDS | 25.0% |
| ! | TATGACAAAAAGATTTGTGA+GGG | - | Chr6:53509072-53509091 | MsG0680032938.01.T01:CDS | 25.0% |
| !! | CGTCAATTTTACATTTTCAA+AGG | - | Chr6:53509183-53509202 | MsG0680032938.01.T01:CDS | 25.0% |
| !!! | GAAAATGTAACATTGATTTC+TGG | + | Chr6:53508686-53508705 | None:intergenic | 25.0% |
| CAAAAATAAAGTCACCATGA+TGG | + | Chr6:53509245-53509264 | None:intergenic | 30.0% | |
| CAATCTATTCCATGATCAAA+CGG | + | Chr6:53508817-53508836 | None:intergenic | 30.0% | |
| CTATGACAAAAAGATTTGTG+AGG | - | Chr6:53509071-53509090 | MsG0680032938.01.T01:CDS | 30.0% | |
| GAGGAGAAAGATATTATTAG+TGG | - | Chr6:53508748-53508767 | MsG0680032938.01.T01:CDS | 30.0% | |
| TAAAACCAATCAGTGAAGAA+TGG | - | Chr6:53509274-53509293 | MsG0680032938.01.T01:CDS | 30.0% | |
| TCAAACATTTCATGAATAGC+AGG | + | Chr6:53509300-53509319 | None:intergenic | 30.0% | |
| TTTCGTGAAAAAACAGTATG+TGG | - | Chr6:53508702-53508721 | MsG0680032938.01.T01:CDS | 30.0% | |
| ! | AAAACGACGAGTAATCTTTT+CGG | + | Chr6:53509100-53509119 | None:intergenic | 30.0% |
| !! | ATGTAACATTGATTTCTGGT+AGG | + | Chr6:53508682-53508701 | None:intergenic | 30.0% |
| !!! | CATCATGGTGACTTTATTTT+TGG | - | Chr6:53509243-53509262 | MsG0680032938.01.T01:CDS | 30.0% |
| AACATGTCAGCAATTGGAAT+AGG | + | Chr6:53508943-53508962 | None:intergenic | 35.0% | |
| ATGGAATAGATGCTGCAATT+AGG | - | Chr6:53508992-53509011 | MsG0680032938.01.T01:CDS | 35.0% | |
| CAATTAGGTACAGGGATAAT+TGG | - | Chr6:53509007-53509026 | MsG0680032938.01.T01:CDS | 35.0% | |
| CACTGTGAGCATCTTTAAAT+AGG | + | Chr6:53509041-53509060 | None:intergenic | 35.0% | |
| ! | GAAAAGATTACTCGTCGTTT+TGG | - | Chr6:53509099-53509118 | MsG0680032938.01.T01:CDS | 35.0% |
| !! | ACGTGGAGAATGTTTGATTT+TGG | + | Chr6:53508896-53508915 | None:intergenic | 35.0% |
| AACCCATCAGTAATATCGCT+CGG | + | Chr6:53508775-53508794 | None:intergenic | 40.0% | |
| AGATGCTGCAATTAGGTACA+GGG | - | Chr6:53508999-53509018 | MsG0680032938.01.T01:CDS | 40.0% | |
| ATGATCAAACGGTTTACCCA+CGG | + | Chr6:53508806-53508825 | None:intergenic | 40.0% | |
| CAAGGAAACATGTCAGCAAT+TGG | + | Chr6:53508949-53508968 | None:intergenic | 40.0% | |
| GCATCTATTCCATGTGCAAA+CGG | + | Chr6:53508985-53509004 | None:intergenic | 40.0% | |
| GTTTGAGAGTGGAATTGATG+CGG | - | Chr6:53509140-53509159 | MsG0680032938.01.T01:CDS | 40.0% | |
| TAGATGCTGCAATTAGGTAC+AGG | - | Chr6:53508998-53509017 | MsG0680032938.01.T01:CDS | 40.0% | |
| TATGGTACAGTGTTTGAGAG+TGG | - | Chr6:53509129-53509148 | MsG0680032938.01.T01:CDS | 40.0% | |
| ! | AACGGTGTTTTCTCAAGACA+AGG | + | Chr6:53508967-53508986 | None:intergenic | 40.0% |
| !! | ATTGATTTCTGGTAGGTGAC+CGG | + | Chr6:53508675-53508694 | None:intergenic | 40.0% |
| !! | GTTTTAGATTATGCTCCAGC+AGG | - | Chr6:53508724-53508743 | MsG0680032938.01.T01:CDS | 40.0% |
| !!! | CGTCGTTTTGGTTGTTTGTA+TGG | - | Chr6:53509111-53509130 | MsG0680032938.01.T01:CDS | 40.0% |
| ATATCTTTCTCCTCACCTGC+TGG | + | Chr6:53508742-53508761 | None:intergenic | 45.0% | |
| GAGAAAACACCGTTTGCACA+TGG | - | Chr6:53508973-53508992 | MsG0680032938.01.T01:CDS | 45.0% | |
| GTGGGTAAACCGTTTGATCA+TGG | - | Chr6:53508805-53508824 | MsG0680032938.01.T01:CDS | 45.0% | |
| TATGCCTCAAACAGCCATCA+TGG | - | Chr6:53509228-53509247 | MsG0680032938.01.T01:CDS | 45.0% | |
| TGATCAAACGGTTTACCCAC+GGG | + | Chr6:53508805-53508824 | None:intergenic | 45.0% | |
| ! | AATGAGGCATTCATCTTCGC+TGG | - | Chr6:53508859-53508878 | MsG0680032938.01.T01:CDS | 45.0% |
| !! | ATACCATTGGTGCTGCATTC+CGG | - | Chr6:53508653-53508672 | MsG0680032938.01.T01:CDS | 45.0% |
| AGATTATGCTCCAGCAGGTG+AGG | - | Chr6:53508729-53508748 | MsG0680032938.01.T01:CDS | 50.0% | |
| AGTATCTCTGCGTCTGAACG+TGG | + | Chr6:53508913-53508932 | None:intergenic | 50.0% | |
| GCAGGCCATTCTTCACTGAT+TGG | + | Chr6:53509282-53509301 | None:intergenic | 50.0% | |
| GGCCGAGCGATATTACTGAT+GGG | - | Chr6:53508770-53508789 | MsG0680032938.01.T01:CDS | 50.0% | |
| GTCACCATGATGGCTGTTTG+AGG | + | Chr6:53509235-53509254 | None:intergenic | 50.0% | |
| AACGGTTTACCCACGGGACA+AGG | + | Chr6:53508799-53508818 | None:intergenic | 55.0% | |
| GGGCCGAGCGATATTACTGA+TGG | - | Chr6:53508769-53508788 | MsG0680032938.01.T01:CDS | 55.0% | |
| TGACCGGAATGCAGCACCAA+TGG | + | Chr6:53508659-53508678 | None:intergenic | 55.0% | |
| ! | GATGGGTTTCCTTGTCCCGT+GGG | - | Chr6:53508787-53508806 | MsG0680032938.01.T01:CDS | 55.0% |
| !! | TGATGGGTTTCCTTGTCCCG+TGG | - | Chr6:53508786-53508805 | MsG0680032938.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 53508643 | 53509332 | 53508643 | ID=MsG0680032938.01;Name=MsG0680032938.01 |
| Chr6 | mRNA | 53508643 | 53509332 | 53508643 | ID=MsG0680032938.01.T01;Parent=MsG0680032938.01;Name=MsG0680032938.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|229 |
| Chr6 | exon | 53508643 | 53509332 | 53508643 | ID=MsG0680032938.01.T01:exon:13138;Parent=MsG0680032938.01.T01 |
| Chr6 | CDS | 53508643 | 53509332 | 53508643 | ID=MsG0680032938.01.T01:cds;Parent=MsG0680032938.01.T01 |
| Gene Sequence |
| Protein sequence |
