Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032942.01.T01 | XP_004488690.1 | 47.111 | 225 | 113 | 4 | 1 | 222 | 1 | 222 | 2.71E-56 | 189 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032942.01.T01 | P08688 | 38.053 | 226 | 130 | 7 | 6 | 223 | 7 | 230 | 6.87E-40 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032942.01.T01 | A0A1S2XHS6 | 47.111 | 225 | 113 | 4 | 1 | 222 | 1 | 222 | 1.29e-56 | 189 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032938.01 | MsG0680032942.01 | 0.807241 | 5.564530e-50 | 3.083577e-47 |
| MsG0680032942.01 | MsG0780036839.01 | 0.829153 | 6.035152e-55 | 6.125394e-52 |
| MsG0680032942.01 | MsG0780039925.01 | 0.801792 | 7.616882e-49 | 3.670261e-46 |
| MsG0680032942.01 | MsG0780040497.01 | 0.804391 | 2.209548e-49 | 1.137851e-46 |
| MsG0680032942.01 | MsG0780040498.01 | 0.802957 | 4.384099e-49 | 2.176109e-46 |
| MsG0680032942.01 | MsG0880047383.01 | 0.801498 | 8.754076e-49 | 4.187037e-46 |
| MsG0480019146.01 | MsG0680032942.01 | 0.820913 | 5.334638e-53 | 4.277281e-50 |
| MsG0480019842.01 | MsG0680032942.01 | 0.800619 | 1.323947e-48 | 6.192983e-46 |
| MsG0380015659.01 | MsG0680032942.01 | 0.821781 | 3.364319e-53 | 2.763718e-50 |
| MsG0380015660.01 | MsG0680032942.01 | 0.810415 | 1.165633e-50 | 7.021903e-48 |
| MsG0380016299.01 | MsG0680032942.01 | 0.804280 | 2.329835e-49 | 1.196398e-46 |
| MsG0380017901.01 | MsG0680032942.01 | 0.808743 | 2.665262e-50 | 1.536066e-47 |
| MsG0580027752.01 | MsG0680032942.01 | 0.808911 | 2.453748e-50 | 1.420465e-47 |
| MsG0580029359.01 | MsG0680032942.01 | 0.802469 | 5.527993e-49 | 2.710035e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032942.01.T01 | MTR_0807s0020 | 44.196 | 224 | 115 | 3 | 1 | 220 | 1 | 218 | 6.58e-59 | 185 |
| MsG0680032942.01.T01 | MTR_6g073540 | 46.222 | 225 | 112 | 3 | 1 | 216 | 1 | 225 | 3.10e-57 | 181 |
| MsG0680032942.01.T01 | MTR_6g045077 | 46.396 | 222 | 108 | 5 | 5 | 220 | 4 | 220 | 1.26e-55 | 177 |
| MsG0680032942.01.T01 | MTR_6g048620 | 46.875 | 224 | 110 | 6 | 5 | 220 | 4 | 226 | 2.05e-55 | 176 |
| MsG0680032942.01.T01 | MTR_1g066530 | 41.892 | 222 | 125 | 2 | 1 | 220 | 1 | 220 | 2.63e-54 | 173 |
| MsG0680032942.01.T01 | MTR_0695s0030 | 42.273 | 220 | 122 | 4 | 6 | 220 | 65 | 284 | 3.39e-54 | 176 |
| MsG0680032942.01.T01 | MTR_6g048910 | 44.843 | 223 | 112 | 5 | 5 | 220 | 4 | 222 | 9.75e-53 | 169 |
| MsG0680032942.01.T01 | MTR_8g038070 | 41.704 | 223 | 125 | 4 | 6 | 223 | 219 | 441 | 1.44e-52 | 176 |
| MsG0680032942.01.T01 | MTR_8g038100 | 43.182 | 220 | 120 | 5 | 6 | 220 | 281 | 500 | 1.79e-52 | 177 |
| MsG0680032942.01.T01 | MTR_6g072470 | 46.231 | 199 | 99 | 2 | 1 | 191 | 1 | 199 | 4.36e-51 | 165 |
| MsG0680032942.01.T01 | MTR_6g072470 | 32.258 | 155 | 91 | 4 | 68 | 209 | 14 | 167 | 4.22e-17 | 77.0 |
| MsG0680032942.01.T01 | MTR_0216s0030 | 42.857 | 196 | 106 | 3 | 6 | 197 | 3 | 196 | 2.74e-49 | 160 |
| MsG0680032942.01.T01 | MTR_0057s0100 | 39.819 | 221 | 126 | 6 | 6 | 220 | 7 | 226 | 1.12e-47 | 157 |
| MsG0680032942.01.T01 | MTR_0057s0170 | 38.914 | 221 | 128 | 6 | 6 | 220 | 7 | 226 | 5.49e-46 | 152 |
| MsG0680032942.01.T01 | MTR_6g040290 | 37.668 | 223 | 131 | 5 | 6 | 220 | 7 | 229 | 3.55e-40 | 137 |
| MsG0680032942.01.T01 | MTR_7g103990 | 39.640 | 222 | 126 | 7 | 6 | 220 | 7 | 227 | 4.74e-39 | 135 |
| MsG0680032942.01.T01 | MTR_7g012000 | 37.438 | 203 | 97 | 4 | 6 | 204 | 7 | 183 | 3.27e-35 | 124 |
| MsG0680032942.01.T01 | MTR_6g048880 | 39.665 | 179 | 98 | 4 | 48 | 220 | 4 | 178 | 1.20e-31 | 114 |
| MsG0680032942.01.T01 | MTR_6g049040 | 44.898 | 147 | 59 | 5 | 1 | 143 | 1 | 129 | 4.30e-31 | 111 |
| MsG0680032942.01.T01 | MTR_6g049040 | 33.333 | 117 | 67 | 2 | 70 | 185 | 16 | 122 | 6.79e-12 | 61.2 |
| MsG0680032942.01.T01 | MTR_6g048810 | 33.333 | 237 | 123 | 5 | 5 | 209 | 4 | 237 | 5.81e-31 | 115 |
| MsG0680032942.01.T01 | MTR_6g048810 | 38.732 | 142 | 81 | 3 | 82 | 220 | 162 | 300 | 1.92e-20 | 87.8 |
| MsG0680032942.01.T01 | MTR_4g102470 | 38.150 | 173 | 80 | 6 | 6 | 172 | 7 | 158 | 1.10e-25 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAACACTAAATTTGAAAA+AGG | 0.257195 | 6:-53529726 | MsG0680032942.01.T01:CDS |
| AATAGGAGCAGCAAACATTT+TGG | 0.257793 | 6:+53529611 | None:intergenic |
| TTTGCTGCTCCTATTAAAAT+CGG | 0.411930 | 6:-53529603 | MsG0680032942.01.T01:CDS |
| AACGATATTGTTAGTGGCCC+TGG | 0.416090 | 6:-53529777 | MsG0680032942.01.T01:CDS |
| TCTACCATCTCATGAAGAAC+AGG | 0.464070 | 6:+53529243 | None:intergenic |
| AATTTAGTGTTAGCAAGCAT+TGG | 0.470401 | 6:+53529735 | None:intergenic |
| AACCCATCAGTAATATCGCC+AGG | 0.476034 | 6:+53529759 | None:intergenic |
| ATTGATATGCGACAGATGAC+TGG | 0.476773 | 6:+53529853 | None:intergenic |
| GGCCCTGGCGATATTACTGA+TGG | 0.515510 | 6:-53529762 | MsG0680032942.01.T01:CDS |
| AACATCAGTGACTATATTGT+CGG | 0.523955 | 6:-53529297 | MsG0680032942.01.T01:CDS |
| GACAATATAGTCACTGATGT+TGG | 0.529971 | 6:+53529299 | None:intergenic |
| TGCAAAGCTCTCTTATTCAA+AGG | 0.541173 | 6:-53529510 | MsG0680032942.01.T01:CDS |
| GAGATGTTTCCTTGTCTCGT+GGG | 0.541614 | 6:-53529579 | MsG0680032942.01.T01:CDS |
| TGAGATGTTTCCTTGTCTCG+TGG | 0.543948 | 6:-53529580 | MsG0680032942.01.T01:CDS |
| TAACACGAATCAGTGCTGAT+TGG | 0.545189 | 6:-53529266 | MsG0680032942.01.T01:CDS |
| TTTCGTGAAAGATCAGTATG+TGG | 0.551905 | 6:-53529823 | MsG0680032942.01.T01:CDS |
| CCTCACAAGACTTTGAGAAG+TGG | 0.554936 | 6:+53529466 | None:intergenic |
| ATCTATTCCATGTTCAAAGT+GGG | 0.555878 | 6:+53529554 | None:intergenic |
| TTGGCCTGTTCTTCATGAGA+TGG | 0.556076 | 6:-53529247 | MsG0680032942.01.T01:CDS |
| TATGGTACAGTGTTTGAGAG+TGG | 0.558207 | 6:-53529408 | MsG0680032942.01.T01:CDS |
| GTGGGAACCCACTTTGAACA+TGG | 0.558929 | 6:-53529561 | MsG0680032942.01.T01:CDS |
| CATCTATTCCATGTTCAAAG+TGG | 0.560169 | 6:+53529553 | None:intergenic |
| AAGTGGGTTCCCACGAGACA+AGG | 0.570328 | 6:+53529570 | None:intergenic |
| ATCATTGTCTTCGACATCAA+AGG | 0.576250 | 6:+53529692 | None:intergenic |
| TGAATAAGAGAGCTTTGCAA+CGG | 0.606631 | 6:+53529514 | None:intergenic |
| GCCCTGGCGATATTACTGAT+GGG | 0.616853 | 6:-53529761 | MsG0680032942.01.T01:CDS |
| CCACTTCTCAAAGTCTTGTG+AGG | 0.641096 | 6:-53529466 | MsG0680032942.01.T01:CDS |
| ATCAGTGACTATATTGTCGG+AGG | 0.644070 | 6:-53529294 | MsG0680032942.01.T01:CDS |
| AAAATGTCACACTTTGCTGT+TGG | 0.645246 | 6:-53529885 | MsG0680032942.01.T01:CDS |
| ACCCATCAGTAATATCGCCA+GGG | 0.652404 | 6:+53529760 | None:intergenic |
| TGCGACAGATGACTGGAATG+CGG | 0.689308 | 6:+53529860 | None:intergenic |
| TGTGCATTAATTGACTACGC+TGG | 0.689959 | 6:-53529324 | MsG0680032942.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GCTAACACTAAATTTGAAAA+AGG | - | Chr6:53529389-53529408 | MsG0680032942.01.T01:CDS | 25.0% |
| !!! | ACGTGGATTGTATTTTATTT+TGG | + | Chr6:53529477-53529496 | None:intergenic | 25.0% |
| AACATCAGTGACTATATTGT+CGG | - | Chr6:53529818-53529837 | MsG0680032942.01.T01:CDS | 30.0% | |
| AATTTAGTGTTAGCAAGCAT+TGG | + | Chr6:53529383-53529402 | None:intergenic | 30.0% | |
| ATCTATTCCATGTTCAAAGT+GGG | + | Chr6:53529564-53529583 | None:intergenic | 30.0% | |
| GCAAAAAACGATATTGTTAG+TGG | - | Chr6:53529332-53529351 | MsG0680032942.01.T01:CDS | 30.0% | |
| TTTGCTGCTCCTATTAAAAT+CGG | - | Chr6:53529512-53529531 | MsG0680032942.01.T01:CDS | 30.0% | |
| ! | AAAACGATGAGCAATCTTTT+CGG | + | Chr6:53529678-53529697 | None:intergenic | 30.0% |
| !!! | AACATCTCACCGATTTTAAT+AGG | + | Chr6:53529524-53529543 | None:intergenic | 30.0% |
| !!! | CATCGTTTTAGTTGTTTGTA+TGG | - | Chr6:53529689-53529708 | MsG0680032942.01.T01:CDS | 30.0% |
| !!! | CGTCGATTTTACATTTTCAA+AGG | - | Chr6:53529761-53529780 | MsG0680032942.01.T01:CDS | 30.0% |
| ATCATTGTCTTCGACATCAA+AGG | + | Chr6:53529426-53529445 | None:intergenic | 35.0% | |
| CATCTATTCCATGTTCAAAG+TGG | + | Chr6:53529565-53529584 | None:intergenic | 35.0% | |
| GACAATATAGTCACTGATGT+TGG | + | Chr6:53529819-53529838 | None:intergenic | 35.0% | |
| TGAATAAGAGAGCTTTGCAA+CGG | + | Chr6:53529604-53529623 | None:intergenic | 35.0% | |
| TGCAAAGCTCTCTTATTCAA+AGG | - | Chr6:53529605-53529624 | MsG0680032942.01.T01:CDS | 35.0% | |
| TTTCGTGAAAGATCAGTATG+TGG | - | Chr6:53529292-53529311 | MsG0680032942.01.T01:CDS | 35.0% | |
| ! | AATAGGAGCAGCAAACATTT+TGG | + | Chr6:53529507-53529526 | None:intergenic | 35.0% |
| ATCAGTGACTATATTGTCGG+AGG | - | Chr6:53529821-53529840 | MsG0680032942.01.T01:CDS | 40.0% | |
| ATTGATATGCGACAGATGAC+TGG | + | Chr6:53529265-53529284 | None:intergenic | 40.0% | |
| TATGGTACAGTGTTTGAGAG+TGG | - | Chr6:53529707-53529726 | MsG0680032942.01.T01:CDS | 40.0% | |
| TCTACCATCTCATGAAGAAC+AGG | + | Chr6:53529875-53529894 | None:intergenic | 40.0% | |
| TGTGCATTAATTGACTACGC+TGG | - | Chr6:53529791-53529810 | MsG0680032942.01.T01:CDS | 40.0% | |
| ! | GATGCAGCTTTTAGTGTGTA+TGG | - | Chr6:53529734-53529753 | MsG0680032942.01.T01:CDS | 40.0% |
| !! | TAACACGAATCAGTGCTGAT+TGG | - | Chr6:53529849-53529868 | MsG0680032942.01.T01:CDS | 40.0% |
| !!! | AACATTTTGGCTTCTGAACG+TGG | + | Chr6:53529494-53529513 | None:intergenic | 40.0% |
| AACCCATCAGTAATATCGCC+AGG | + | Chr6:53529359-53529378 | None:intergenic | 45.0% | |
| AACGATATTGTTAGTGGCCC+TGG | - | Chr6:53529338-53529357 | MsG0680032942.01.T01:CDS | 45.0% | |
| ACCCATCAGTAATATCGCCA+GGG | + | Chr6:53529358-53529377 | None:intergenic | 45.0% | |
| GAGATGTTTCCTTGTCTCGT+GGG | - | Chr6:53529536-53529555 | MsG0680032942.01.T01:CDS | 45.0% | |
| TGAGATGTTTCCTTGTCTCG+TGG | - | Chr6:53529535-53529554 | MsG0680032942.01.T01:CDS | 45.0% | |
| TTGGCCTGTTCTTCATGAGA+TGG | - | Chr6:53529868-53529887 | MsG0680032942.01.T01:CDS | 45.0% | |
| ! | CCACTTCTCAAAGTCTTGTG+AGG | - | Chr6:53529649-53529668 | MsG0680032942.01.T01:CDS | 45.0% |
| ! | CCTCACAAGACTTTGAGAAG+TGG | + | Chr6:53529652-53529671 | None:intergenic | 45.0% |
| GCCCTGGCGATATTACTGAT+GGG | - | Chr6:53529354-53529373 | MsG0680032942.01.T01:CDS | 50.0% | |
| GTGGGAACCCACTTTGAACA+TGG | - | Chr6:53529554-53529573 | MsG0680032942.01.T01:CDS | 50.0% | |
| TGCGACAGATGACTGGAATG+CGG | + | Chr6:53529258-53529277 | None:intergenic | 50.0% | |
| GGCCCTGGCGATATTACTGA+TGG | - | Chr6:53529353-53529372 | MsG0680032942.01.T01:CDS | 55.0% | |
| ! | AAGTGGGTTCCCACGAGACA+AGG | + | Chr6:53529548-53529567 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 53529233 | 53529904 | 53529233 | ID=MsG0680032942.01;Name=MsG0680032942.01 |
| Chr6 | mRNA | 53529233 | 53529904 | 53529233 | ID=MsG0680032942.01.T01;Parent=MsG0680032942.01;Name=MsG0680032942.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|223 |
| Chr6 | exon | 53529233 | 53529904 | 53529233 | ID=MsG0680032942.01.T01:exon:2122;Parent=MsG0680032942.01.T01 |
| Chr6 | CDS | 53529233 | 53529904 | 53529233 | ID=MsG0680032942.01.T01:cds;Parent=MsG0680032942.01.T01 |
| Gene Sequence |
| Protein sequence |
