AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032953.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032953.01.T02 MTR_6g048440 97.764 313 7 0 1 313 1 313 0.0 637
MsG0680032953.01.T02 MTR_6g048440 97.569 288 7 0 1 288 1 288 0.0 588
MsG0680032953.01.T02 MTR_6g048440 97.407 270 7 0 1 270 1 270 0.0 551
MsG0680032953.01.T02 MTR_6g048440 97.357 227 6 0 1 227 1 227 4.69e-166 461
MsG0680032953.01.T02 MTR_1g100070 69.937 316 72 3 1 313 1 296 1.68e-150 424
MsG0680032953.01.T02 MTR_1g100070 65.000 340 72 4 1 313 1 320 6.64e-145 411
MsG0680032953.01.T02 MTR_1g100070 67.033 273 67 3 1 270 1 253 1.99e-127 365
MsG0680032953.01.T01 MTR_6g048440 97.764 313 7 0 1 313 1 313 0.0 637
MsG0680032953.01.T01 MTR_6g048440 97.569 288 7 0 1 288 1 288 0.0 588
MsG0680032953.01.T01 MTR_6g048440 97.407 270 7 0 1 270 1 270 0.0 551
MsG0680032953.01.T01 MTR_6g048440 97.357 227 6 0 1 227 1 227 4.69e-166 461
MsG0680032953.01.T01 MTR_1g100070 69.937 316 72 3 1 313 1 296 1.68e-150 424
MsG0680032953.01.T01 MTR_1g100070 65.000 340 72 4 1 313 1 320 6.64e-145 411
MsG0680032953.01.T01 MTR_1g100070 67.033 273 67 3 1 270 1 253 1.99e-127 365
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032953.01.T02 AT4G25700 64.650 314 97 4 1 313 1 301 3.11e-146 414
MsG0680032953.01.T02 AT5G52570 64.423 312 95 6 1 311 1 297 1.01e-132 379
MsG0680032953.01.T02 AT4G25700 57.895 228 82 4 1 227 1 215 7.85e-88 262
MsG0680032953.01.T02 AT4G25700 57.895 228 82 4 1 227 1 215 7.85e-88 262
MsG0680032953.01.T02 AT5G52570 84.884 86 13 0 226 311 143 228 2.17e-39 139
MsG0680032953.01.T02 AT5G52570 41.989 181 81 8 1 174 1 164 1.50e-32 120
MsG0680032953.01.T01 AT4G25700 64.650 314 97 4 1 313 1 301 3.11e-146 414
MsG0680032953.01.T01 AT5G52570 64.423 312 95 6 1 311 1 297 1.01e-132 379
MsG0680032953.01.T01 AT4G25700 57.895 228 82 4 1 227 1 215 7.85e-88 262
MsG0680032953.01.T01 AT4G25700 57.895 228 82 4 1 227 1 215 7.85e-88 262
MsG0680032953.01.T01 AT5G52570 84.884 86 13 0 226 311 143 228 2.17e-39 139
MsG0680032953.01.T01 AT5G52570 41.989 181 81 8 1 174 1 164 1.50e-32 120

Find 25 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTTTGTAGTAACGGTTTA+AGG 0.141677 6:-53764758 None:intergenic
CTTTGATGGAAACATCTTAA+AGG 0.219951 6:-53764829 None:intergenic
TCCATTTGTGTGTCACGTTT+TGG 0.270912 6:-53764910 None:intergenic
TTTGATGGAAACATCTTAAA+GGG 0.354826 6:-53764828 None:intergenic
TAGCCTTGGTATAACATCTA+TGG 0.360829 6:+53765068 MsG0680032953.01.T01:CDS
TAAGGGTTGAATTGTTATAG+TGG 0.369441 6:-53764740 None:intergenic
CTGCCGCCATAGGTGAAGTT+TGG 0.378599 6:-53764708 None:intergenic
TTATTCTCTTGAAGAGTTGT+TGG 0.388038 6:-53764949 None:intergenic
CTTCACACTTTGTGTTCTCA+TGG 0.423292 6:+53764882 MsG0680032953.01.T01:CDS
GGTTTGTAGTAACGGTTTAA+GGG 0.425120 6:-53764757 None:intergenic
TCCAAAACGTGACACACAAA+TGG 0.461950 6:+53764909 MsG0680032953.01.T01:CDS
GTAACAAAGGTTTGTAGTAA+CGG 0.484640 6:-53764765 None:intergenic
GGGAGAGAGAGGGAGAGGGA+TGG 0.484724 6:-53764607 None:intergenic
GCGGTTAGTCCTGCCGCCAT+AGG 0.499360 6:-53764718 None:intergenic
GACAAGATAAGTGTTGGTGT+TGG 0.534487 6:-53764805 None:intergenic
GCAGCTGTTATGTCTAGCCT+TGG 0.540668 6:+53765054 MsG0680032953.01.T01:CDS
CGGAGATGATAAGGTAACAA+AGG 0.545005 6:-53764778 None:intergenic
TGTATATGTGAATGTGAAGA+TGG 0.553058 6:-53764573 None:intergenic
AAAGGGGACAAGATAAGTGT+TGG 0.574250 6:-53764811 None:intergenic
CAAACTTCACCTATGGCGGC+AGG 0.581620 6:+53764709 MsG0680032953.01.T01:exon
AAATGGCAGAGAAATTAGCT+AGG 0.608932 6:+53765001 MsG0680032953.01.T01:CDS
TTGATGGAAACATCTTAAAG+GGG 0.613022 6:-53764827 None:intergenic
AAACCAAACTTCACCTATGG+CGG 0.627582 6:+53764705 MsG0680032953.01.T01:exon
GGGTTGAATTGTTATAGTGG+CGG 0.628849 6:-53764737 None:intergenic
CAGCCATAGATGTTATACCA+AGG 0.717570 6:-53765071 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAATGGAAAAT+TGG - Chr6:53765439-53765458 None:intergenic 10.0%
!! AAAAATATTAGAGTTTAATA+TGG + Chr6:53767140-53767159 MsG0680032953.01.T01:intron 10.0%
!! AAAATTAAAAACTATTACAT+CGG - Chr6:53765270-53765289 None:intergenic 10.0%
!! AAATTAGTTAAATATGAAAA+TGG + Chr6:53767293-53767312 MsG0680032953.01.T01:intron 10.0%
!! AGAAAAAATTATAAGAAAAT+TGG + Chr6:53767196-53767215 MsG0680032953.01.T01:intron 10.0%
!! TAAATTGAATATATTTCTTT+AGG + Chr6:53768358-53768377 MsG0680032953.01.T01:intron 10.0%
!!! AATTTTTTTTTCTACTTTTA+GGG - Chr6:53765957-53765976 None:intergenic 10.0%
!!! ATTAGTTATTTGATTATTTT+TGG + Chr6:53768177-53768196 MsG0680032953.01.T01:intron 10.0%
!!! TTAGTTATTTGATTATTTTT+GGG + Chr6:53768178-53768197 MsG0680032953.01.T01:intron 10.0%
!! AAATCAAACAAATATTCATA+TGG - Chr6:53766338-53766357 None:intergenic 15.0%
!! AAATTAAAAACAAAACAGAA+AGG - Chr6:53766668-53766687 None:intergenic 15.0%
!! AATCAAACAAATATTCATAT+GGG - Chr6:53766337-53766356 None:intergenic 15.0%
!! ACTAATGTATATAAGAAAAT+TGG - Chr6:53765476-53765495 None:intergenic 15.0%
!! ATGATTTAAACATTTATCAT+TGG - Chr6:53766692-53766711 None:intergenic 15.0%
!! CTTTAATATAGAAATAACAT+GGG - Chr6:53767083-53767102 None:intergenic 15.0%
!! TCTTTAATATAGAAATAACA+TGG - Chr6:53767084-53767103 None:intergenic 15.0%
!!! AAATATGAATCGAAAATTTT+AGG + Chr6:53767556-53767575 MsG0680032953.01.T01:intron 15.0%
!!! AATTATTTTAATTTGAGAGT+TGG + Chr6:53766014-53766033 MsG0680032953.01.T01:intron 15.0%
!!! AATTTTCTTATATACATTAG+TGG + Chr6:53765475-53765494 MsG0680032953.01.T01:intron 15.0%
!!! ATGTTAATTTTTCGATATTT+CGG + Chr6:53767746-53767765 MsG0680032953.01.T01:intron 15.0%
!!! ATTATTTTAATTTGAGAGTT+GGG + Chr6:53766015-53766034 MsG0680032953.01.T01:intron 15.0%
!!! ATTTTTTTTTCTACTTTTAG+GGG - Chr6:53765956-53765975 None:intergenic 15.0%
!!! CACTTTTTTAATATTTAACT+AGG - Chr6:53767654-53767673 None:intergenic 15.0%
!!! GAATTTTTTTTTCTACTTTT+AGG - Chr6:53765958-53765977 None:intergenic 15.0%
!!! GATGCATTTAATTTTAAATT+TGG - Chr6:53765212-53765231 None:intergenic 15.0%
!!! TAGTTATTTGATTATTTTTG+GGG + Chr6:53768179-53768198 MsG0680032953.01.T01:intron 15.0%
!!! TATTTGTTTGATTTTGATTT+TGG + Chr6:53766344-53766363 MsG0680032953.01.T01:intron 15.0%
!!! TCTTTTAATTTAGTTCAATT+AGG + Chr6:53767523-53767542 MsG0680032953.01.T01:intron 15.0%
!!! TTTTTTAGTTAACTTTGTTA+GGG - Chr6:53767258-53767277 None:intergenic 15.0%
!!! TTTTTTATCAAGTGTATTTT+TGG + Chr6:53765718-53765737 MsG0680032953.01.T01:intron 15.0%
!! AAAATACACAAATAGAAGAT+TGG + Chr6:53765608-53765627 MsG0680032953.01.T01:intron 20.0%
!! AAAATTAGTTAAAGCAAACA+AGG - Chr6:53766270-53766289 None:intergenic 20.0%
!! AAGATATTCTATTTAGTGTT+TGG + Chr6:53767444-53767463 MsG0680032953.01.T01:intron 20.0%
!! AATAAAAGACAAGAAAATCA+TGG - Chr6:53768220-53768239 None:intergenic 20.0%
!! AGATATTCTATTTAGTGTTT+GGG + Chr6:53767445-53767464 MsG0680032953.01.T01:intron 20.0%
!! ATACCAACAAAATATTAAGT+AGG + Chr6:53767708-53767727 MsG0680032953.01.T01:intron 20.0%
!! ATTGAATATATTTCTTTAGG+TGG + Chr6:53768361-53768380 MsG0680032953.01.T01:intron 20.0%
!! CAATGAAAACCATAAATTAA+GGG - Chr6:53766615-53766634 None:intergenic 20.0%
!! GTATATGTTTAAATTATCAG+TGG - Chr6:53765817-53765836 None:intergenic 20.0%
!! TATCTTAACAACAAAAAAAG+TGG - Chr6:53765566-53765585 None:intergenic 20.0%
!! TCTATATATAAGAGAAAGTT+TGG + Chr6:53765171-53765190 MsG0680032953.01.T01:intron 20.0%
!!! ACTAGGTATATTTAATTTTC+AGG + Chr6:53766071-53766090 MsG0680032953.01.T01:intron 20.0%
!!! ATAGTTTAGAACATGTAATA+GGG - Chr6:53766188-53766207 None:intergenic 20.0%
!!! ATTAATTGTTTTATTCCTCT+AGG + Chr6:53765536-53765555 MsG0680032953.01.T01:intron 20.0%
!!! CTAAACTATCCAATAATTTT+TGG + Chr6:53766199-53766218 MsG0680032953.01.T01:intron 20.0%
!!! CTGTTTATTTAAGATTTTCA+TGG + Chr6:53765100-53765119 MsG0680032953.01.T01:CDS 20.0%
!!! CTTTTAGAAGTCTTTTTTTA+GGG + Chr6:53765981-53766000 MsG0680032953.01.T01:intron 20.0%
!!! CTTTTTTAGTTAACTTTGTT+AGG - Chr6:53767259-53767278 None:intergenic 20.0%
!!! GTGTGTACATTATTAATTTT+GGG + Chr6:53766037-53766056 MsG0680032953.01.T01:intron 20.0%
!!! TCTTTTAGAAGTCTTTTTTT+AGG + Chr6:53765980-53765999 MsG0680032953.01.T01:intron 20.0%
!!! TTATTTTTTCATATGCACAT+TGG + Chr6:53765295-53765314 MsG0680032953.01.T01:intron 20.0%
!!! TTTTAGAAGTCTTTTTTTAG+GGG + Chr6:53765982-53766001 MsG0680032953.01.T01:intron 20.0%
!!! TTTTGTTGACCAAAAATTAT+TGG - Chr6:53766211-53766230 None:intergenic 20.0%
! AAAAACTATTACATCGGATA+CGG - Chr6:53765264-53765283 None:intergenic 25.0%
! AAAATACTAACAAGTGTCAT+AGG - Chr6:53767021-53767040 None:intergenic 25.0%
! AAAGTTTGGTCAATGATATT+CGG + Chr6:53765185-53765204 MsG0680032953.01.T01:intron 25.0%
! AAGGATAAGAAATTACTTTG+TGG - Chr6:53768098-53768117 None:intergenic 25.0%
! AGGATAAGAAATTACTTTGT+GGG - Chr6:53768097-53768116 None:intergenic 25.0%
! ATACAAGAATACATTACCTT+TGG - Chr6:53768298-53768317 None:intergenic 25.0%
! ATCAAATAGTTCATAACCAT+TGG - Chr6:53766386-53766405 None:intergenic 25.0%
! ATGTAATAGGGGAAATTAAT+TGG - Chr6:53766176-53766195 None:intergenic 25.0%
! ATGTTTAAATTATCAGTGGT+GGG - Chr6:53765813-53765832 None:intergenic 25.0%
! CCAATGAAAACCATAAATTA+AGG - Chr6:53766616-53766635 None:intergenic 25.0%
! CTTTCTCTTATATATAGAAC+TGG - Chr6:53765169-53765188 None:intergenic 25.0%
! GTAACGTATAACTAAATGTT+AGG + Chr6:53767676-53767695 MsG0680032953.01.T01:intron 25.0%
! GTATAACTAAATGTTAGGTA+TGG + Chr6:53767681-53767700 MsG0680032953.01.T01:intron 25.0%
! GTGATAAGAATGTTATGATA+AGG - Chr6:53768117-53768136 None:intergenic 25.0%
! TATGTTTAAATTATCAGTGG+TGG - Chr6:53765814-53765833 None:intergenic 25.0%
! TTTGATGGAAACATCTTAAA+GGG - Chr6:53764831-53764850 None:intergenic 25.0%
!! CAACATTTTACAATAACCAA+TGG + Chr6:53766367-53766386 MsG0680032953.01.T01:intron 25.0%
!! CCTTAATTTATGGTTTTCAT+TGG + Chr6:53766613-53766632 MsG0680032953.01.T01:intron 25.0%
!! GATAGTTTAGAACATGTAAT+AGG - Chr6:53766189-53766208 None:intergenic 25.0%
!! GATCTTTACTCTTTTTCTAT+AGG + Chr6:53767218-53767237 MsG0680032953.01.T01:intron 25.0%
!! GCTAATATGAATCTGAAATT+GGG + Chr6:53765329-53765348 MsG0680032953.01.T01:intron 25.0%
!! GGTGTGTACATTATTAATTT+TGG + Chr6:53766036-53766055 MsG0680032953.01.T01:intron 25.0%
!! GTTCCTACTTAATATTTTGT+TGG - Chr6:53767714-53767733 None:intergenic 25.0%
!! TAGTTTAGAACATGTAATAG+GGG - Chr6:53766187-53766206 None:intergenic 25.0%
!! TGCTAATATGAATCTGAAAT+TGG + Chr6:53765328-53765347 MsG0680032953.01.T01:intron 25.0%
!!! AGAACTTGTCACTTTTTAAT+AGG + Chr6:53767581-53767600 MsG0680032953.01.T01:intron 25.0%
!!! AGGTATATTTAATTTTCAGG+TGG + Chr6:53766074-53766093 MsG0680032953.01.T01:intron 25.0%
!!! CTATTTTTTTAACTCGAGTT+CGG - Chr6:53766763-53766782 None:intergenic 25.0%
!!! GGTATATTTAATTTTCAGGT+GGG + Chr6:53766075-53766094 MsG0680032953.01.T01:intron 25.0%
!!! TGTCATTTAATTTGCATGTT+AGG + Chr6:53767397-53767416 MsG0680032953.01.T01:intron 25.0%
!!! TTGATTATTTTTGGGGTATA+AGG + Chr6:53768186-53768205 MsG0680032953.01.T01:intron 25.0%
!!! TTTAAGATTTTCATGGCAAA+TGG + Chr6:53765107-53765126 MsG0680032953.01.T01:CDS 25.0%
!!! TTTCAACCTTTGTGTTTTTA+AGG - Chr6:53764860-53764879 None:intergenic 25.0%
!!! TTTGAATTTTGAAGCATGTT+TGG - Chr6:53766247-53766266 None:intergenic 25.0%
!!! TTTTTAAGGTTGTTCTTTGA+TGG - Chr6:53764846-53764865 None:intergenic 25.0%
AAAAAAAGTGGAAATCCTAG+AGG - Chr6:53765554-53765573 None:intergenic 30.0%
AAAAAACCAAACTTCACCTA+TGG + Chr6:53764702-53764721 MsG0680032953.01.T01:exon 30.0%
AAAACAAAACAGAAAGGTCA+CGG - Chr6:53766662-53766681 None:intergenic 30.0%
ACACTTATTAGCATGATCTA+AGG - Chr6:53766876-53766895 None:intergenic 30.0%
ACCATTCAGACAAATTTGAT+GGG + Chr6:53768248-53768267 MsG0680032953.01.T01:CDS 30.0%
AGCACATAAGAAAGATACAA+AGG - Chr6:53768333-53768352 None:intergenic 30.0%
ATACACAAATAGAAGATTGG+TGG + Chr6:53765611-53765630 MsG0680032953.01.T01:intron 30.0%
ATACGGTATAGGAGAAAATT+TGG - Chr6:53765247-53765266 None:intergenic 30.0%
CTTTGATGGAAACATCTTAA+AGG - Chr6:53764832-53764851 None:intergenic 30.0%
GAAAATGGAAGTGAAAACAT+GGG + Chr6:53767308-53767327 MsG0680032953.01.T01:intron 30.0%
GAACAACCTTAAAAACACAA+AGG + Chr6:53764851-53764870 MsG0680032953.01.T01:CDS 30.0%
GTAACAAAGGTTTGTAGTAA+CGG - Chr6:53764768-53764787 None:intergenic 30.0%
GTTATGAACTATTTGATACG+TGG + Chr6:53766389-53766408 MsG0680032953.01.T01:intron 30.0%
TAAGGGTTGAATTGTTATAG+TGG - Chr6:53764743-53764762 None:intergenic 30.0%
TATGTGTCTCCCTTAATTTA+TGG + Chr6:53766603-53766622 MsG0680032953.01.T01:intron 30.0%
TCAAATGAGATAAACACAAG+TGG - Chr6:53765639-53765658 None:intergenic 30.0%
TCAAGTTATCTCACCAAAAA+TGG + Chr6:53764984-53765003 MsG0680032953.01.T01:CDS 30.0%
TGAAAATGGAAGTGAAAACA+TGG + Chr6:53767307-53767326 MsG0680032953.01.T01:intron 30.0%
TGTGAAAGAAACCAAAGTTA+AGG - Chr6:53766550-53766569 None:intergenic 30.0%
TGTGCATGAAATAGCAAAAA+AGG - Chr6:53767484-53767503 None:intergenic 30.0%
TTATTCTCTTGAAGAGTTGT+TGG - Chr6:53764952-53764971 None:intergenic 30.0%
TTGATGGAAACATCTTAAAG+GGG - Chr6:53764830-53764849 None:intergenic 30.0%
TTTGGGCAATAAATTCAACT+AGG + Chr6:53766054-53766073 MsG0680032953.01.T01:intron 30.0%
! AACTTTGGTTTCTTTCACAA+AGG + Chr6:53766551-53766570 MsG0680032953.01.T01:CDS 30.0%
! CATGCTAATAAGTGTTTCTA+AGG + Chr6:53766881-53766900 MsG0680032953.01.T01:intron 30.0%
! TATGAATCTGAAATTGGGTT+TGG + Chr6:53765334-53765353 MsG0680032953.01.T01:intron 30.0%
!! AAATTGGGTTTGGTTTGTTT+AGG + Chr6:53765344-53765363 MsG0680032953.01.T01:intron 30.0%
!! AATTGGGTTTGGTTTGTTTA+GGG + Chr6:53765345-53765364 MsG0680032953.01.T01:intron 30.0%
!! CATTTAATTTGCATGTTAGG+TGG + Chr6:53767400-53767419 MsG0680032953.01.T01:intron 30.0%
!!! AGAAGTGTTGTTTTTTTGGT+TGG + Chr6:53767350-53767369 MsG0680032953.01.T01:intron 30.0%
!!! AGGTGAAGTTTGGTTTTTTA+AGG - Chr6:53764701-53764720 None:intergenic 30.0%
!!! ATTTAATTTTCAGGTGGGAA+TGG + Chr6:53766080-53766099 MsG0680032953.01.T01:intron 30.0%
!!! ATTTTTGGTGAGATAACTTG+AGG - Chr6:53764985-53765004 None:intergenic 30.0%
!!! GGAACTTGTCACTTTTTTAT+AGG + Chr6:53767606-53767625 MsG0680032953.01.T01:intron 30.0%
!!! TCGAAGAAGTGTTGTTTTTT+TGG + Chr6:53767346-53767365 MsG0680032953.01.T01:intron 30.0%
!!! TGTTAGGTGGATACATTTTT+TGG + Chr6:53767413-53767432 MsG0680032953.01.T01:intron 30.0%
!!! TTGGCTTTGAAGCTAAATTT+TGG + Chr6:53767369-53767388 MsG0680032953.01.T01:intron 30.0%
AAACAAAACAGAAAGGTCAC+GGG - Chr6:53766661-53766680 None:intergenic 35.0%
AAATGGCAGAGAAATTAGCT+AGG + Chr6:53765001-53765020 MsG0680032953.01.T01:CDS 35.0%
AGCTAGAGAAAGAGATAAGT+AGG + Chr6:53768535-53768554 MsG0680032953.01.T01:CDS 35.0%
AGGTTTGTAGTAACGGTTTA+AGG - Chr6:53764761-53764780 None:intergenic 35.0%
ATTTCATGCACACTTTGTGA+AGG + Chr6:53767492-53767511 MsG0680032953.01.T01:intron 35.0%
CACCATTCAGACAAATTTGA+TGG + Chr6:53768247-53768266 MsG0680032953.01.T01:CDS 35.0%
CCATTCAGACAAATTTGATG+GGG + Chr6:53768249-53768268 MsG0680032953.01.T01:CDS 35.0%
CGACTCTATAAGAATTGACT+TGG - Chr6:53766815-53766834 None:intergenic 35.0%
GAACTGGAGCGAAAAATTTA+AGG - Chr6:53765153-53765172 None:intergenic 35.0%
GGTTTGTAGTAACGGTTTAA+GGG - Chr6:53764760-53764779 None:intergenic 35.0%
GTCTTGGAATTACGGTATTT+GGG + Chr6:53767977-53767996 MsG0680032953.01.T01:CDS 35.0%
TAGAGAAAGAGATAAGTAGG+AGG + Chr6:53768538-53768557 MsG0680032953.01.T01:CDS 35.0%
TATTACATCGGATACGGTAT+AGG - Chr6:53765258-53765277 None:intergenic 35.0%
TGGTTTCTTTCACAAAGGAT+TGG + Chr6:53766556-53766575 MsG0680032953.01.T01:CDS 35.0%
! AAGATTTTCATGGCAAATGG+AGG + Chr6:53765110-53765129 MsG0680032953.01.T01:CDS 35.0%
! ATTCCTTGGTCTGAAATGTT+TGG + Chr6:53765378-53765397 MsG0680032953.01.T01:CDS 35.0%
! TAACCTATGATAACTTGTGC+AGG + Chr6:53768485-53768504 MsG0680032953.01.T01:intron 35.0%
! TAGCCTTGGTATAACATCTA+TGG + Chr6:53765068-53765087 MsG0680032953.01.T01:CDS 35.0%
! TTATTAGCATGATCTAAGGC+TGG - Chr6:53766872-53766891 None:intergenic 35.0%
! TTTTATAGGCAGGAACTTGT+CGG + Chr6:53767620-53767639 MsG0680032953.01.T01:intron 35.0%
!! AGAAATAACATGGGCTAGTT+AGG - Chr6:53767074-53767093 None:intergenic 35.0%
!! ATTGGGTTTGGTTTGTTTAG+GGG + Chr6:53765346-53765365 MsG0680032953.01.T01:intron 35.0%
!!! CTTGTCACTTTTTAATAGGC+AGG + Chr6:53767585-53767604 MsG0680032953.01.T01:intron 35.0%
!!! CTTGTCACTTTTTTATAGGC+AGG + Chr6:53767610-53767629 MsG0680032953.01.T01:intron 35.0%
!!! GCTAATTTCTCTGCCATTTT+TGG - Chr6:53765000-53765019 None:intergenic 35.0%
!!! TAAGTGTTGGTGTTGGTTTT+CGG - Chr6:53764801-53764820 None:intergenic 35.0%
AAACCAAACTTCACCTATGG+CGG + Chr6:53764705-53764724 MsG0680032953.01.T01:exon 40.0%
AAAGGGGACAAGATAAGTGT+TGG - Chr6:53764814-53764833 None:intergenic 40.0%
AAATAGGGCACATTGGCAAT+GGG - Chr6:53768057-53768076 None:intergenic 40.0%
AAGACCCTGCTCGTAAATAA+GGG - Chr6:53767962-53767981 None:intergenic 40.0%
AATGGAAGTGAAAACATGGG+AGG + Chr6:53767311-53767330 MsG0680032953.01.T01:intron 40.0%
AATGGAGGTAAGTAGTGTGA+TGG + Chr6:53765125-53765144 MsG0680032953.01.T01:intron 40.0%
ATGGAAGTGAAAACATGGGA+GGG + Chr6:53767312-53767331 MsG0680032953.01.T01:intron 40.0%
CAATGTGCCCTATTTCAGAA+GGG + Chr6:53768062-53768081 MsG0680032953.01.T01:CDS 40.0%
CAGCCATAGATGTTATACCA+AGG - Chr6:53765074-53765093 None:intergenic 40.0%
CATGTTTGTACACGATGGAT+TGG + Chr6:53768008-53768027 MsG0680032953.01.T01:CDS 40.0%
CCCCATCAAATTTGTCTGAA+TGG - Chr6:53768252-53768271 None:intergenic 40.0%
CGGAGATGATAAGGTAACAA+AGG - Chr6:53764781-53764800 None:intergenic 40.0%
CTTCACACTTTGTGTTCTCA+TGG + Chr6:53764882-53764901 MsG0680032953.01.T01:CDS 40.0%
GGGTTGAATTGTTATAGTGG+CGG - Chr6:53764740-53764759 None:intergenic 40.0%
GGTACATTTGCTCTATCAGT+GGG + Chr6:53765399-53765418 MsG0680032953.01.T01:CDS 40.0%
GGTCTTGGAATTACGGTATT+TGG + Chr6:53767976-53767995 MsG0680032953.01.T01:CDS 40.0%
GTACCAAACATTTCAGACCA+AGG - Chr6:53765384-53765403 None:intergenic 40.0%
GTTCATAGGAGATTCTCAGT+GGG + Chr6:53768030-53768049 MsG0680032953.01.T01:CDS 40.0%
GTTCCTGCACAAGTTATCAT+AGG - Chr6:53768491-53768510 None:intergenic 40.0%
TCCAAAACGTGACACACAAA+TGG + Chr6:53764909-53764928 MsG0680032953.01.T01:CDS 40.0%
TCCATCGTGTACAAACATGT+AGG - Chr6:53768007-53768026 None:intergenic 40.0%
TCCTTCTCTTGCTCTATGAT+GGG - Chr6:53766471-53766490 None:intergenic 40.0%
TGGAATTACGGTATTTGGGA+TGG + Chr6:53767981-53768000 MsG0680032953.01.T01:CDS 40.0%
TGGTACATTTGCTCTATCAG+TGG + Chr6:53765398-53765417 MsG0680032953.01.T01:CDS 40.0%
! AAATTTGATGGGGTGCCATA+TGG + Chr6:53768259-53768278 MsG0680032953.01.T01:CDS 40.0%
! AATTTGATGGGGTGCCATAT+GGG + Chr6:53768260-53768279 MsG0680032953.01.T01:CDS 40.0%
! ATAACTTGTGCAGGAACTTG+AGG + Chr6:53768494-53768513 MsG0680032953.01.T01:intron 40.0%
! GGTGGGATTGTCAAGAATTT+TGG - Chr6:53765796-53765815 None:intergenic 40.0%
! TCCATTTGTGTGTCACGTTT+TGG - Chr6:53764913-53764932 None:intergenic 40.0%
! TTTCTAGAGAGAGAGAGAGA+GGG - Chr6:53764638-53764657 None:intergenic 40.0%
!! GACAAGATAAGTGTTGGTGT+TGG - Chr6:53764808-53764827 None:intergenic 40.0%
!! TACACGATGGATTGGTTCAT+AGG + Chr6:53768016-53768035 MsG0680032953.01.T01:CDS 40.0%
!!! AATGGAGTTTTGGGCTAGAT+GGG + Chr6:53766098-53766117 MsG0680032953.01.T01:CDS 40.0%
!!! GTTGGTTTTCGGAGATGATA+AGG - Chr6:53764790-53764809 None:intergenic 40.0%
AAGTTAAGGAGAGCGATAGC+AGG - Chr6:53766536-53766555 None:intergenic 45.0%
ATGGGTCTTGCTTCAACTTG+TGG - Chr6:53766319-53766338 None:intergenic 45.0%
CAAGACCCTGCTCGTAAATA+AGG - Chr6:53767963-53767982 None:intergenic 45.0%
CCAATGTGCCCTATTTCAGA+AGG + Chr6:53768061-53768080 MsG0680032953.01.T01:CDS 45.0%
CTCCTTCTCTTGCTCTATGA+TGG - Chr6:53766472-53766491 None:intergenic 45.0%
CTTATTTACGAGCAGGGTCT+TGG + Chr6:53767961-53767980 MsG0680032953.01.T01:intron 45.0%
GAAATAGGGCACATTGGCAA+TGG - Chr6:53768058-53768077 None:intergenic 45.0%
GCCTACATGTTTGTACACGA+TGG + Chr6:53768003-53768022 MsG0680032953.01.T01:CDS 45.0%
GCTATCGCTCTCCTTAACTT+TGG + Chr6:53766536-53766555 MsG0680032953.01.T01:CDS 45.0%
GGTTCATAGGAGATTCTCAG+TGG + Chr6:53768029-53768048 MsG0680032953.01.T01:CDS 45.0%
TCCCATCATAGAGCAAGAGA+AGG + Chr6:53766467-53766486 MsG0680032953.01.T01:CDS 45.0%
! GTCACCCTTATTTACGAGCA+GGG + Chr6:53767955-53767974 MsG0680032953.01.T01:intron 45.0%
! GTTTCTAGAGAGAGAGAGAG+AGG - Chr6:53764639-53764658 None:intergenic 45.0%
! TCAGGTGGGAATGGAGTTTT+GGG + Chr6:53766089-53766108 MsG0680032953.01.T01:intron 45.0%
! TGTCACCCTTATTTACGAGC+AGG + Chr6:53767954-53767973 MsG0680032953.01.T01:intron 45.0%
! TTCAGGTGGGAATGGAGTTT+TGG + Chr6:53766088-53766107 MsG0680032953.01.T01:intron 45.0%
!! CCTTCTGAAATAGGGCACAT+TGG - Chr6:53768064-53768083 None:intergenic 45.0%
!! GCAGCTACCCTTCTGAAATA+GGG - Chr6:53768072-53768091 None:intergenic 45.0%
!! GTTTGGTTTGTTTAGGGGAG+TGG + Chr6:53765351-53765370 MsG0680032953.01.T01:intron 45.0%
!! GTTTTCATTGGCTACCCTTG+CGG + Chr6:53766625-53766644 MsG0680032953.01.T01:intron 45.0%
!! TGCAGCTACCCTTCTGAAAT+AGG - Chr6:53768073-53768092 None:intergenic 45.0%
!! TTTCACAAAGGATTGGTCCC+TGG + Chr6:53766563-53766582 MsG0680032953.01.T01:CDS 45.0%
!!! GAATGGAGTTTTGGGCTAGA+TGG + Chr6:53766097-53766116 MsG0680032953.01.T01:CDS 45.0%
!!! GTGCCATATGGGCTGTTTTT+GGG + Chr6:53768271-53768290 MsG0680032953.01.T01:CDS 45.0%
!! ATCAAAATAAAAAAAAAAAA+TGG - Chr6:53765447-53765466 None:intergenic 5.0%
!!! TAAATTTTTCTATATAAATT+TGG + Chr6:53767042-53767061 MsG0680032953.01.T01:intron 5.0%
AGGAACTTGAGGAAGTGGGA+GGG + Chr6:53768505-53768524 MsG0680032953.01.T01:intron 50.0%
AGGGGAGTGGAGAAATTCCT+TGG + Chr6:53765364-53765383 MsG0680032953.01.T01:intron 50.0%
CGAGCAGGGTCTTGGAATTA+CGG + Chr6:53767969-53767988 MsG0680032953.01.T01:intron 50.0%
CTCTTTGGCATGCTTCCTTG+TGG + Chr6:53766130-53766149 MsG0680032953.01.T01:CDS 50.0%
GCAGCTGTTATGTCTAGCCT+TGG + Chr6:53765054-53765073 MsG0680032953.01.T01:CDS 50.0%
GGTCCCAAAAACAGCCCATA+TGG - Chr6:53768277-53768296 None:intergenic 50.0%
GTGCAGGAACTTGAGGAAGT+GGG + Chr6:53768501-53768520 MsG0680032953.01.T01:intron 50.0%
TACCTCATGCATGTGCCACA+AGG - Chr6:53766148-53766167 None:intergenic 50.0%
TGTGCAGGAACTTGAGGAAG+TGG + Chr6:53768500-53768519 MsG0680032953.01.T01:intron 50.0%
TTCCTTGTGGCACATGCATG+AGG + Chr6:53766143-53766162 MsG0680032953.01.T01:CDS 50.0%
! TTGGTCCCTGGTCTTTGCTT+TGG + Chr6:53766575-53766594 MsG0680032953.01.T01:CDS 50.0%
!! GGTGCCATATGGGCTGTTTT+TGG + Chr6:53768270-53768289 MsG0680032953.01.T01:CDS 50.0%
!!! TGGGCTGTTTTTGGGACCAA+AGG + Chr6:53768279-53768298 MsG0680032953.01.T01:CDS 50.0%
ACCGCACCAAAGCAAAGACC+AGG - Chr6:53766584-53766603 None:intergenic 55.0%
AGAGAGAGAGAGAGAGGGAG+AGG - Chr6:53764633-53764652 None:intergenic 55.0%
AGAGAGAGAGAGAGGGAGAG+GGG - Chr6:53764631-53764650 None:intergenic 55.0%
ATTGGCTACCCTTGCGGTGA+AGG + Chr6:53766631-53766650 MsG0680032953.01.T01:intron 55.0%
CAAACTTCACCTATGGCGGC+AGG + Chr6:53764709-53764728 MsG0680032953.01.T01:exon 55.0%
CAGGAACTTGAGGAAGTGGG+AGG + Chr6:53768504-53768523 MsG0680032953.01.T01:intron 55.0%
CCGCACCAAAGCAAAGACCA+GGG - Chr6:53766583-53766602 None:intergenic 55.0%
CTGCCGCCATAGGTGAAGTT+TGG - Chr6:53764711-53764730 None:intergenic 55.0%
GAGAGAGAGAGAGAGGGAGA+GGG - Chr6:53764632-53764651 None:intergenic 55.0%
TTGGCTACCCTTGCGGTGAA+GGG + Chr6:53766632-53766651 MsG0680032953.01.T01:intron 55.0%
! CCCTGGTCTTTGCTTTGGTG+CGG + Chr6:53766580-53766599 MsG0680032953.01.T01:CDS 55.0%
! GATGGGCTCACAAGGCTCTT+TGG + Chr6:53766115-53766134 MsG0680032953.01.T01:CDS 55.0%
! TTGGGCTAGATGGGCTCACA+AGG + Chr6:53766107-53766126 MsG0680032953.01.T01:CDS 55.0%
ACGGGTAACCCTTCACCGCA+AGG - Chr6:53766643-53766662 None:intergenic 60.0%
CGGGTAACCCTTCACCGCAA+GGG - Chr6:53766642-53766661 None:intergenic 60.0%
GAGAGAGAGAGAGGGAGAGG+GGG - Chr6:53764630-53764649 None:intergenic 60.0%
AGAGGGAGAGGGGGAGAGAG+AGG - Chr6:53764621-53764640 None:intergenic 65.0%
AGAGGGGGAGAGAGAGGGAG+AGG - Chr6:53764615-53764634 None:intergenic 65.0%
GAGGGAGAGGGGGAGAGAGA+GGG - Chr6:53764620-53764639 None:intergenic 65.0%
GAGGGGGAGAGAGAGGGAGA+GGG - Chr6:53764614-53764633 None:intergenic 65.0%
GGGAGAGAGAGGGAGAGGGA+TGG - Chr6:53764610-53764629 None:intergenic 65.0%
! GCGGTTAGTCCTGCCGCCAT+AGG - Chr6:53764721-53764740 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 53764575 53768671 53764575 ID=MsG0680032953.01;Name=MsG0680032953.01
Chr6 mRNA 53764581 53768671 53764581 ID=MsG0680032953.01.T01;Parent=MsG0680032953.01;Name=MsG0680032953.01.T01;_AED=0.37;_eAED=0.37;_QI=118|1|1|1|0.85|0.75|8|87|313
Chr6 exon 53764643 53765131 53764643 ID=MsG0680032953.01.T01:exon:9457;Parent=MsG0680032953.01.T01
Chr6 exon 53764581 53764620 53764581 ID=MsG0680032953.01.T01:exon:9456;Parent=MsG0680032953.01.T01
Chr6 five_prime_UTR 53764581 53764620 53764581 ID=MsG0680032953.01.T01:five_prime_utr;Parent=MsG0680032953.01.T01
Chr6 five_prime_UTR 53764643 53764720 53764643 ID=MsG0680032953.01.T01:five_prime_utr;Parent=MsG0680032953.01.T01
Chr6 CDS 53764721 53765131 53764721 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 CDS 53765366 53765428 53765366 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 CDS 53766093 53766164 53766093 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 CDS 53766467 53766601 53766467 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 CDS 53767976 53768101 53767976 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 CDS 53768244 53768300 53768244 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 CDS 53768507 53768584 53768507 ID=MsG0680032953.01.T01:cds;Parent=MsG0680032953.01.T01
Chr6 three_prime_UTR 53768585 53768671 53768585 ID=MsG0680032953.01.T01:three_prime_utr;Parent=MsG0680032953.01.T01
Chr6 mRNA 53764575 53768671 53764575 ID=MsG0680032953.01.T02;Parent=MsG0680032953.01;Name=MsG0680032953.01.T02;_AED=0.36;_eAED=0.36;_QI=146|1|1|1|1|1|7|87|313
Chr6 exon 53764575 53765131 53764575 ID=MsG0680032953.01.T02:exon:9464;Parent=MsG0680032953.01.T02
Chr6 five_prime_UTR 53764575 53764720 53764575 ID=MsG0680032953.01.T02:five_prime_utr;Parent=MsG0680032953.01.T02
Chr6 CDS 53764721 53765131 53764721 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 CDS 53765366 53765428 53765366 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 CDS 53766093 53766164 53766093 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 CDS 53766467 53766601 53766467 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 CDS 53767976 53768101 53767976 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 CDS 53768244 53768300 53768244 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 CDS 53768507 53768584 53768507 ID=MsG0680032953.01.T02:cds;Parent=MsG0680032953.01.T02
Chr6 three_prime_UTR 53768585 53768671 53768585 ID=MsG0680032953.01.T02:three_prime_utr;Parent=MsG0680032953.01.T02
Gene Sequence

>MsG0680032953.01.T02

ATGGCGGCAGGACTAACCGCCACTATAACAATTCAACCCTTAAACCGTTACTACAAACCTTTGTTACCTTATCATCTCCGAAAACCAACACCAACACTTATCTTGTCCCCTTTAAGATGTTTCCATCAAAGAACAACCTTAAAAACACAAAGGTTGAAAAGCTTCACACTTTGTGTTCTCATGGAAGATCCAAAACGTGACACACAAATGGAAATTGAAGAAGAAAAACCAACAACTCTTCAAGAGAATAATTATATTATTCCTCAAGTTATCTCACCAAAAATGGCAGAGAAATTAGCTAGGAAAAAATCAGAGAGATTTACGTATCTTGTTGCAGCTGTTATGTCTAGCCTTGGTATAACATCTATGGCTGTGTTGTCTGTTTATTTAAGATTTTCATGGCAAATGGAGGGGAGTGGAGAAATTCCTTGGTCTGAAATGTTTGGTACATTTGCTCTATCAGTGGGTGCTGCTGTGGGAATGGAGTTTTGGGCTAGATGGGCTCACAAGGCTCTTTGGCATGCTTCCTTGTGGCACATGCATGAGTCCCATCATAGAGCAAGAGAAGGAGCTTTCGAGTTGAATGATGTTTTTGCAATAATCAATGCTGTTCCTGCTATCGCTCTCCTTAACTTTGGTTTCTTTCACAAAGGATTGGTCCCTGGTCTTTGCTTTGGTGCGGGTCTTGGAATTACGGTATTTGGGATGGCCTACATGTTTGTACACGATGGATTGGTTCATAGGAGATTCTCAGTGGGACCCATTGCCAATGTGCCCTATTTCAGAAGGGTAGCTGCAGCCCACAAACTTCACCATTCAGACAAATTTGATGGGGTGCCATATGGGCTGTTTTTGGGACCAAAGGAACTTGAGGAAGTGGGAGGGCTAGAAGAGCTAGAGAAAGAGATAAGTAGGAGGACAAAATCATACAATAGTTCATAA

>MsG0680032953.01.T01

ATGGCGGCAGGACTAACCGCCACTATAACAATTCAACCCTTAAACCGTTACTACAAACCTTTGTTACCTTATCATCTCCGAAAACCAACACCAACACTTATCTTGTCCCCTTTAAGATGTTTCCATCAAAGAACAACCTTAAAAACACAAAGGTTGAAAAGCTTCACACTTTGTGTTCTCATGGAAGATCCAAAACGTGACACACAAATGGAAATTGAAGAAGAAAAACCAACAACTCTTCAAGAGAATAATTATATTATTCCTCAAGTTATCTCACCAAAAATGGCAGAGAAATTAGCTAGGAAAAAATCAGAGAGATTTACGTATCTTGTTGCAGCTGTTATGTCTAGCCTTGGTATAACATCTATGGCTGTGTTGTCTGTTTATTTAAGATTTTCATGGCAAATGGAGGGGAGTGGAGAAATTCCTTGGTCTGAAATGTTTGGTACATTTGCTCTATCAGTGGGTGCTGCTGTGGGAATGGAGTTTTGGGCTAGATGGGCTCACAAGGCTCTTTGGCATGCTTCCTTGTGGCACATGCATGAGTCCCATCATAGAGCAAGAGAAGGAGCTTTCGAGTTGAATGATGTTTTTGCAATAATCAATGCTGTTCCTGCTATCGCTCTCCTTAACTTTGGTTTCTTTCACAAAGGATTGGTCCCTGGTCTTTGCTTTGGTGCGGGTCTTGGAATTACGGTATTTGGGATGGCCTACATGTTTGTACACGATGGATTGGTTCATAGGAGATTCTCAGTGGGACCCATTGCCAATGTGCCCTATTTCAGAAGGGTAGCTGCAGCCCACAAACTTCACCATTCAGACAAATTTGATGGGGTGCCATATGGGCTGTTTTTGGGACCAAAGGAACTTGAGGAAGTGGGAGGGCTAGAAGAGCTAGAGAAAGAGATAAGTAGGAGGACAAAATCATACAATAGTTCATAA
Protein sequence

>MsG0680032953.01.T02

MAAGLTATITIQPLNRYYKPLLPYHLRKPTPTLILSPLRCFHQRTTLKTQRLKSFTLCVLMEDPKRDTQMEIEEEKPTTLQENNYIIPQVISPKMAEKLARKKSERFTYLVAAVMSSLGITSMAVLSVYLRFSWQMEGSGEIPWSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHRAREGAFELNDVFAIINAVPAIALLNFGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFSVGPIANVPYFRRVAAAHKLHHSDKFDGVPYGLFLGPKELEEVGGLEELEKEISRRTKSYNSS*

>MsG0680032953.01.T01

MAAGLTATITIQPLNRYYKPLLPYHLRKPTPTLILSPLRCFHQRTTLKTQRLKSFTLCVLMEDPKRDTQMEIEEEKPTTLQENNYIIPQVISPKMAEKLARKKSERFTYLVAAVMSSLGITSMAVLSVYLRFSWQMEGSGEIPWSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHRAREGAFELNDVFAIINAVPAIALLNFGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFSVGPIANVPYFRRVAAAHKLHHSDKFDGVPYGLFLGPKELEEVGGLEELEKEISRRTKSYNSS*