AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033028.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033028.01.T01 MTR_6g047800 95.294 170 8 0 1 170 1 170 3.48e-115 332
MsG0680033028.01.T01 MTR_6g047800 91.358 81 6 1 168 247 229 309 1.96e-36 131
MsG0680033028.01.T01 MTR_6g047800 95.031 161 8 0 10 170 1 161 1.75e-108 315
MsG0680033028.01.T01 MTR_6g047800 91.358 81 6 1 168 247 220 300 1.66e-36 131
MsG0680033028.01.T01 MTR_2g014590 76.471 170 40 0 1 170 2 171 6.94e-95 278
MsG0680033028.01.T01 MTR_2g014570 70.968 62 13 3 185 246 6 62 6.86e-19 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033028.01.T01 AT5G14600 58.413 315 63 5 1 247 1 315 1.09e-119 344

Find 69 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTCATTGTTATGTATT+TGG 0.122846 6:-55170605 MsG0680033028.01.T01:CDS
ACAATTATGTGGAAGGTTTC+TGG 0.176634 6:-55168419 MsG0680033028.01.T01:three_prime_UTR
GATGAAGAGGTTTGATTTAA+TGG 0.223569 6:-55168297 MsG0680033028.01.T01:three_prime_UTR
AACAATTAGTAACGGTGATT+TGG 0.271982 6:-55170854 MsG0680033028.01.T01:CDS
TCCCAATTCTTCTTCTGTTA+TGG 0.281081 6:-55168590 MsG0680033028.01.T01:CDS
GGTTGTGTCGTTCTTGAATC+TGG 0.284355 6:-55170571 MsG0680033028.01.T01:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 6:-55168704 MsG0680033028.01.T01:CDS
GTCTAAAGTATTCAACAATA+AGG 0.308450 6:-55170716 MsG0680033028.01.T01:CDS
TTGTTTATGTGAAAGATATA+AGG 0.311677 6:-55168753 MsG0680033028.01.T01:intron
CCAAACATGAATTTCCTATA+TGG 0.311905 6:+55168384 None:intergenic
AAAACTAAAGTCCACAATTC+AGG 0.328439 6:+55170664 None:intergenic
GATTGGATAGGAAAGTCATT+TGG 0.331486 6:-55170739 MsG0680033028.01.T01:CDS
CTTGAATCTGGCACTGGAAG+TGG 0.346516 6:-55170559 MsG0680033028.01.T01:CDS
AGCCATAACAGAAGAAGAAT+TGG 0.358884 6:+55168588 None:intergenic
ATGAAGAGGTTTGATTTAAT+GGG 0.372610 6:-55168296 MsG0680033028.01.T01:three_prime_UTR
GGTATTTGTAATCTCCATAT+AGG 0.372906 6:-55168398 MsG0680033028.01.T01:three_prime_UTR
ATGTATTTGGAGATTGTTCC+TGG 0.385811 6:-55170592 MsG0680033028.01.T01:CDS
GATCCCTCAGATACCGTTAC+TGG 0.416695 6:+55170799 None:intergenic
CCATATAGGAAATTCATGTT+TGG 0.417762 6:-55168384 MsG0680033028.01.T01:three_prime_UTR
ATTGGATAGGAAAGTCATTT+GGG 0.422230 6:-55170738 MsG0680033028.01.T01:CDS
GACGGTAATGATGGCTCTAA+TGG 0.423313 6:-55168667 MsG0680033028.01.T01:CDS
AGGGATGACTTTGAGAGGAT+TGG 0.424669 6:-55169082 MsG0680033028.01.T01:CDS
TAATTCTGTATTACAGTCAC+TGG 0.463847 6:-55168319 MsG0680033028.01.T01:three_prime_UTR
TCTAAAGTATTCAACAATAA+GGG 0.468151 6:-55170715 MsG0680033028.01.T01:CDS
CTTAATTCACAACAATTATG+TGG 0.469309 6:-55168430 MsG0680033028.01.T01:three_prime_UTR
TTGAATCTGGCACTGGAAGT+GGG 0.495210 6:-55170558 MsG0680033028.01.T01:CDS
AAGAGGTTTGATTTAATGGG+TGG 0.498099 6:-55168293 MsG0680033028.01.T01:three_prime_UTR
GCCATAACAGAAGAAGAATT+GGG 0.501842 6:+55168589 None:intergenic
GTCGTTCTTGAATCTGGCAC+TGG 0.503209 6:-55170565 MsG0680033028.01.T01:CDS
CTTTAACTACTTCACTTGCA+AGG 0.505822 6:-55170534 MsG0680033028.01.T01:CDS
ATTTGGCAATTGTTTATGAA+AGG 0.507354 6:-55170837 MsG0680033028.01.T01:CDS
TTTAAACATTCAGATTGGAT+AGG 0.513874 6:-55170751 MsG0680033028.01.T01:CDS
ATTCACAACAATTATGTGGA+AGG 0.514332 6:-55168426 MsG0680033028.01.T01:three_prime_UTR
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 6:-55170517 MsG0680033028.01.T01:CDS
TACTTGTTTATTGAAAATGT+TGG 0.523271 6:-55170911 MsG0680033028.01.T01:exon
TCAGAGATTGACGGTAATGA+TGG 0.525850 6:-55168676 MsG0680033028.01.T01:CDS
CTTGTAGGGATGACTTTGAG+AGG 0.533942 6:-55169087 MsG0680033028.01.T01:intron
GCGGGAGTGAGGGACATTCA+AGG 0.551907 6:-55169040 MsG0680033028.01.T01:intron
TAGCTCCTACTCCTGAATTG+TGG 0.553993 6:-55170675 MsG0680033028.01.T01:CDS
AAAGTCCACAATTCAGGAGT+AGG 0.563658 6:+55170670 None:intergenic
GAACGTTGCCTCCTCTTGCA+AGG 0.567462 6:+55168637 None:intergenic
ATAAGCAACTTAGTCACTGC+GGG 0.567539 6:-55169058 MsG0680033028.01.T01:CDS
CTTAGTCACTGCGGGAGTGA+GGG 0.568888 6:-55169050 MsG0680033028.01.T01:CDS
ACTTAGTCACTGCGGGAGTG+AGG 0.570411 6:-55169051 MsG0680033028.01.T01:CDS
GAAGCCAGTAACGGTATCTG+AGG 0.571902 6:-55170803 MsG0680033028.01.T01:CDS
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 6:-55168648 MsG0680033028.01.T01:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 6:-55170533 MsG0680033028.01.T01:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 6:-55168645 MsG0680033028.01.T01:CDS
ATTACAGTCACTGGATGAAG+AGG 0.584950 6:-55168310 MsG0680033028.01.T01:three_prime_UTR
GATTCAAGAACGACACAACC+AGG 0.585429 6:+55170574 None:intergenic
ATGGAAAGCTCAGAGATTGA+CGG 0.586050 6:-55168685 MsG0680033028.01.T01:CDS
TGACCTCTAGCTTCACCGCA+TGG 0.587593 6:+55168562 None:intergenic
AGTAACGGTATCTGAGGGAT+CGG 0.588437 6:-55170797 MsG0680033028.01.T01:CDS
TTTAATAGAACAATTAGTAA+CGG 0.596180 6:-55170862 MsG0680033028.01.T01:CDS
AAGAGGAGGCAACGTTCAGA+TGG 0.606722 6:-55168631 MsG0680033028.01.T01:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 6:+55170502 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 6:-55168712 MsG0680033028.01.T01:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 6:+55168716 None:intergenic
ACGTTCAGATGGAGCTAGCG+TGG 0.623569 6:-55168620 MsG0680033028.01.T01:CDS
GGACATACGAAGTTCGAGAA+GGG 0.625372 6:-55168711 MsG0680033028.01.T01:CDS
AACAAAGATCTGGATCAGCT+AGG 0.636973 6:-55170468 MsG0680033028.01.T01:intron
AGACCATGCGGTGAAGCTAG+AGG 0.638675 6:-55168565 MsG0680033028.01.T01:CDS
AAAGTATTCAACAATAAGGG+CGG 0.646223 6:-55170712 MsG0680033028.01.T01:CDS
TATATAGAATCTGTGTCCTG+TGG 0.646737 6:+55170638 None:intergenic
TGACATCATGAAGCCAGTAA+CGG 0.657116 6:-55170812 MsG0680033028.01.T01:CDS
AAGCCAGTAACGGTATCTGA+GGG 0.657172 6:-55170802 MsG0680033028.01.T01:CDS
TCTGTTATGGCTAGACCATG+CGG 0.664012 6:-55168577 MsG0680033028.01.T01:CDS
GGTGAAGCTAGAGGTCACAC+TGG 0.703760 6:-55168556 MsG0680033028.01.T01:CDS
TATAAGCAACTTAGTCACTG+CGG 0.729959 6:-55169059 MsG0680033028.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACTTATATATTGTATTA+CGG + Chr6:55169738-55169757 None:intergenic 10.0%
!! AGTCAATTATATATAAAACA+AGG + Chr6:55169799-55169818 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG - Chr6:55168326-55168345 MsG0680033028.01.T01:three_prime_UTR 15.0%
!!! TTTTTACAATGAAAAAACTT+AGG - Chr6:55169685-55169704 MsG0680033028.01.T01:intron 15.0%
!! AACATATAAAAGAGGAAAAT+CGG + Chr6:55170362-55170381 None:intergenic 20.0%
!! ACATAAACAAACATATACAA+AGG + Chr6:55170425-55170444 None:intergenic 20.0%
!! ATTTATTTAACTGTACTCAA+TGG - Chr6:55170016-55170035 MsG0680033028.01.T01:intron 20.0%
!! GTAATTGCAAGTAATATTAT+TGG - Chr6:55169949-55169968 MsG0680033028.01.T01:intron 20.0%
!! TAAAAATAAACTGTTGTATG+AGG + Chr6:55170737-55170756 None:intergenic 20.0%
!! TACTTGTTTATTGAAAATGT+TGG - Chr6:55168277-55168296 MsG0680033028.01.T01:three_prime_UTR 20.0%
!! TTGTTTATGTGAAAGATATA+AGG - Chr6:55170435-55170454 MsG0680033028.01.T01:intron 20.0%
!! TTTATTTAACTGTACTCAAT+GGG - Chr6:55170017-55170036 MsG0680033028.01.T01:intron 20.0%
!!! TCTAAAGTATTCAACAATAA+GGG - Chr6:55168473-55168492 MsG0680033028.01.T01:three_prime_UTR 20.0%
! ACATATAAAAGAGGAAAATC+GGG + Chr6:55170361-55170380 None:intergenic 25.0%
! AGTTGCATAACATATAAAAG+AGG + Chr6:55170370-55170389 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG - Chr6:55168777-55168796 MsG0680033028.01.T01:intron 25.0%
! ATTTGGCAATTGTTTATGAA+AGG - Chr6:55168351-55168370 MsG0680033028.01.T01:three_prime_UTR 25.0%
! CTACCAAAAAACTAGAATAA+CGG + Chr6:55168907-55168926 None:intergenic 25.0%
! CTTAATTCACAACAATTATG+TGG - Chr6:55170758-55170777 MsG0680033028.01.T01:CDS 25.0%
! TGAAATAGAAGATAGTGTTT+CGG + Chr6:55169855-55169874 None:intergenic 25.0%
! TTATTTAACTGTACTCAATG+GGG - Chr6:55170018-55170037 MsG0680033028.01.T01:intron 25.0%
! TTTAAACATTCAGATTGGAT+AGG - Chr6:55168437-55168456 MsG0680033028.01.T01:three_prime_UTR 25.0%
!! ATGAAGAGGTTTGATTTAAT+GGG - Chr6:55170892-55170911 MsG0680033028.01.T01:CDS 25.0%
!! GTCTAAAGTATTCAACAATA+AGG - Chr6:55168472-55168491 MsG0680033028.01.T01:three_prime_UTR 25.0%
!!! GAGAATGATTTTTTCAATAG+CGG - Chr6:55169464-55169483 MsG0680033028.01.T01:intron 25.0%
!!! GTGTTTTTAAACATTCAGAT+TGG - Chr6:55168432-55168451 MsG0680033028.01.T01:three_prime_UTR 25.0%
AAAACTAAAGTCCACAATTC+AGG + Chr6:55168527-55168546 None:intergenic 30.0%
AAAATCACGATCAAACTGAT+GGG + Chr6:55169220-55169239 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG - Chr6:55168334-55168353 MsG0680033028.01.T01:three_prime_UTR 30.0%
ACTGTAATGGTGTGTTTATA+TGG + Chr6:55169002-55169021 None:intergenic 30.0%
ATTCACAACAATTATGTGGA+AGG - Chr6:55170762-55170781 MsG0680033028.01.T01:CDS 30.0%
ATTGGATAGGAAAGTCATTT+GGG - Chr6:55168450-55168469 MsG0680033028.01.T01:three_prime_UTR 30.0%
ATTGTAAAAATAAGCCACGT+TGG + Chr6:55169675-55169694 None:intergenic 30.0%
CAGCTTCATTGTTATGTATT+TGG - Chr6:55168583-55168602 MsG0680033028.01.T01:CDS 30.0%
CCAAACATGAATTTCCTATA+TGG + Chr6:55170807-55170826 None:intergenic 30.0%
CCATATAGGAAATTCATGTT+TGG - Chr6:55170804-55170823 MsG0680033028.01.T01:CDS 30.0%
CTATCTTCTATTTCACATAG+CGG - Chr6:55169860-55169879 MsG0680033028.01.T01:intron 30.0%
GAAATAGAAGATAGTGTTTC+GGG + Chr6:55169854-55169873 None:intergenic 30.0%
GACTTATTTCAACTGTTATG+CGG - Chr6:55169815-55169834 MsG0680033028.01.T01:intron 30.0%
GCTGATATACGAACTATATT+GGG - Chr6:55169595-55169614 MsG0680033028.01.T01:intron 30.0%
GGTATTTGTAATCTCCATAT+AGG - Chr6:55170790-55170809 MsG0680033028.01.T01:CDS 30.0%
GTGTCATCAATAGCATATTA+CGG + Chr6:55169891-55169910 None:intergenic 30.0%
TAATTCTGTATTACAGTCAC+TGG - Chr6:55170869-55170888 MsG0680033028.01.T01:CDS 30.0%
TATTGTATTACGGTGAATAC+AGG + Chr6:55169728-55169747 None:intergenic 30.0%
TTGTAAAAATAAGCCACGTT+GGG + Chr6:55169674-55169693 None:intergenic 30.0%
TTTAACTACTTCACTTGCAA+GGG - Chr6:55168655-55168674 MsG0680033028.01.T01:CDS 30.0%
! AAAGTATTCAACAATAAGGG+CGG - Chr6:55168476-55168495 MsG0680033028.01.T01:three_prime_UTR 30.0%
! AGACAAAAGAGAATTTTAGG+CGG - Chr6:55168946-55168965 MsG0680033028.01.T01:intron 30.0%
! AGCAGACAAAAGAGAATTTT+AGG - Chr6:55168943-55168962 MsG0680033028.01.T01:intron 30.0%
! ATACGATTTTCAGACTGTAA+TGG + Chr6:55169015-55169034 None:intergenic 30.0%
! ATTTGATTTCCATGAGCATA+GGG - Chr6:55169350-55169369 MsG0680033028.01.T01:intron 30.0%
! CAGTCTGAAAATCGTATTTT+TGG - Chr6:55169018-55169037 MsG0680033028.01.T01:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG - Chr6:55170445-55170464 MsG0680033028.01.T01:intron 30.0%
! GATGAAGAGGTTTGATTTAA+TGG - Chr6:55170891-55170910 MsG0680033028.01.T01:CDS 30.0%
! TTTCTAGTGTTGTCAACTAT+TGG - Chr6:55168818-55168837 MsG0680033028.01.T01:intron 30.0%
!! ATACTTGTTTTTCTCCTTGT+AGG - Chr6:55170086-55170105 MsG0680033028.01.T01:intron 30.0%
!! ATCTTGCAATTCATTGATTC+AGG + Chr6:55169096-55169115 None:intergenic 30.0%
!! TACTTGTTTTTCTCCTTGTA+GGG - Chr6:55170087-55170106 MsG0680033028.01.T01:intron 30.0%
!!! AGTGTTGCAGTTTTTTTTAG+CGG + Chr6:55168872-55168891 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG - Chr6:55168901-55168920 MsG0680033028.01.T01:intron 30.0%
AACGTATAAACATGTCCTGT+AGG + Chr6:55168689-55168708 None:intergenic 35.0%
ACAATTATGTGGAAGGTTTC+TGG - Chr6:55170769-55170788 MsG0680033028.01.T01:CDS 35.0%
ACACATGTATCAAACAACAG+AGG + Chr6:55169424-55169443 None:intergenic 35.0%
ACCCTGCATGCAAATAAAAA+GGG + Chr6:55169775-55169794 None:intergenic 35.0%
ACTTGAGATGTTCAGTGTAA+AGG - Chr6:55170047-55170066 MsG0680033028.01.T01:intron 35.0%
AGCCATAACAGAAGAAGAAT+TGG + Chr6:55170603-55170622 None:intergenic 35.0%
ATAAACATGCCCTTCACAAA+TGG + Chr6:55169328-55169347 None:intergenic 35.0%
ATGTATTTGGAGATTGTTCC+TGG - Chr6:55168596-55168615 MsG0680033028.01.T01:CDS 35.0%
CAAAATCACGATCAAACTGA+TGG + Chr6:55169221-55169240 None:intergenic 35.0%
CGTTGACATTCTGAACATTA+GGG + Chr6:55169985-55170004 None:intergenic 35.0%
CGTTGTACTTGTTCAATACA+CGG + Chr6:55170300-55170319 None:intergenic 35.0%
CTTTAACTACTTCACTTGCA+AGG - Chr6:55168654-55168673 MsG0680033028.01.T01:CDS 35.0%
GATTGGATAGGAAAGTCATT+TGG - Chr6:55168449-55168468 MsG0680033028.01.T01:three_prime_UTR 35.0%
GCAAAAATGTTACGACATGA+CGG - Chr6:55170255-55170274 MsG0680033028.01.T01:intron 35.0%
GCATGCAAATAAAAAGGGAA+TGG + Chr6:55169770-55169789 None:intergenic 35.0%
GCCATAACAGAAGAAGAATT+GGG + Chr6:55170602-55170621 None:intergenic 35.0%
GGCTGATATACGAACTATAT+TGG - Chr6:55169594-55169613 MsG0680033028.01.T01:intron 35.0%
GGTTATCTTCCTTGAAATTC+CGG + Chr6:55169075-55169094 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG + Chr6:55169074-55169093 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG + Chr6:55170299-55170318 None:intergenic 35.0%
TATAAGCAACTTAGTCACTG+CGG - Chr6:55170129-55170148 MsG0680033028.01.T01:intron 35.0%
TATATAGAATCTGTGTCCTG+TGG + Chr6:55168553-55168572 None:intergenic 35.0%
TATGTAATACTACACCAGCT+AGG - Chr6:55169383-55169402 MsG0680033028.01.T01:intron 35.0%
TCCCAATTCTTCTTCTGTTA+TGG - Chr6:55170598-55170617 MsG0680033028.01.T01:CDS 35.0%
TTGTACTTGTTCAATACACG+GGG + Chr6:55170298-55170317 None:intergenic 35.0%
! AAGAGGTTTGATTTAATGGG+TGG - Chr6:55170895-55170914 MsG0680033028.01.T01:CDS 35.0%
! CATTTGATTTCCATGAGCAT+AGG - Chr6:55169349-55169368 MsG0680033028.01.T01:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG - Chr6:55168710-55168729 MsG0680033028.01.T01:CDS 35.0%
! GGTGGAGATAATGTGATTTT+AGG - Chr6:55170913-55170932 MsG0680033028.01.T01:exon 35.0%
! TCGGTTTTACAGAATCGATT+CGG - Chr6:55168410-55168429 MsG0680033028.01.T01:three_prime_UTR 35.0%
! TGCTATTGATGACACTTTCA+TGG - Chr6:55169896-55169915 MsG0680033028.01.T01:intron 35.0%
!! ATCCCTTCATAAATTTGCTG+TGG - Chr6:55169262-55169281 MsG0680033028.01.T01:intron 35.0%
!! TCCCTTTTTATTTGCATGCA+GGG - Chr6:55169771-55169790 MsG0680033028.01.T01:intron 35.0%
!! TTCCCTTTTTATTTGCATGC+AGG - Chr6:55169770-55169789 MsG0680033028.01.T01:intron 35.0%
!!! ATCGTATTTTTGGTGAAACG+TGG - Chr6:55169028-55169047 MsG0680033028.01.T01:intron 35.0%
AAAGTCCACAATTCAGGAGT+AGG + Chr6:55168521-55168540 None:intergenic 40.0%
AACAAAGATCTGGATCAGCT+AGG - Chr6:55168720-55168739 MsG0680033028.01.T01:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG - Chr6:55170330-55170349 MsG0680033028.01.T01:intron 40.0%
ACCGCAGAAAATTGTAGGTA+CGG + Chr6:55169123-55169142 None:intergenic 40.0%
ACTATAGTGCTATCACATGG+TGG - Chr6:55169488-55169507 MsG0680033028.01.T01:intron 40.0%
ATAAGCAACTTAGTCACTGC+GGG - Chr6:55170130-55170149 MsG0680033028.01.T01:intron 40.0%
ATCAAACAACAGAGGAAGTC+AGG + Chr6:55169416-55169435 None:intergenic 40.0%
ATGGAAAGCTCAGAGATTGA+CGG - Chr6:55170503-55170522 MsG0680033028.01.T01:CDS 40.0%
ATTACAGTCACTGGATGAAG+AGG - Chr6:55170878-55170897 MsG0680033028.01.T01:CDS 40.0%
CACCCTGCATGCAAATAAAA+AGG + Chr6:55169776-55169795 None:intergenic 40.0%
CGCCACAGCAAATTTATGAA+GGG + Chr6:55169267-55169286 None:intergenic 40.0%
GTATGGTCGGAATCAGAATT+TGG + Chr6:55169163-55169182 None:intergenic 40.0%
TCAGAGATTGACGGTAATGA+TGG - Chr6:55170512-55170531 MsG0680033028.01.T01:CDS 40.0%
TGACATCATGAAGCCAGTAA+CGG - Chr6:55168376-55168395 MsG0680033028.01.T01:three_prime_UTR 40.0%
! ACCGTACCTACAATTTTCTG+CGG - Chr6:55169119-55169138 MsG0680033028.01.T01:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG + Chr6:55170249-55170268 None:intergenic 40.0%
! GCGTTGACATTCTGAACATT+AGG + Chr6:55169986-55170005 None:intergenic 40.0%
! GGCTGCTATTAAAGTTTTGC+TGG - Chr6:55169573-55169592 MsG0680033028.01.T01:intron 40.0%
! TTTTGGATCTACCTCAACCT+TGG - Chr6:55170214-55170233 MsG0680033028.01.T01:intron 40.0%
!! CCTTCATAAATTTGCTGTGG+CGG - Chr6:55169265-55169284 MsG0680033028.01.T01:intron 40.0%
!! CGTATGATTTTGATACCCCT+TGG + Chr6:55169195-55169214 None:intergenic 40.0%
!! CTATTGGCTCTATAGCATAG+CGG - Chr6:55168834-55168853 MsG0680033028.01.T01:intron 40.0%
!! CTTCATAAATTTGCTGTGGC+GGG - Chr6:55169266-55169285 MsG0680033028.01.T01:intron 40.0%
!! TTTTTGGTGAAACGTGGTCA+TGG - Chr6:55169034-55169053 MsG0680033028.01.T01:intron 40.0%
!!! ATTTTAGGCGGTGTTGTCAA+TGG - Chr6:55168958-55168977 MsG0680033028.01.T01:intron 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG - Chr6:55169228-55169247 MsG0680033028.01.T01:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG - Chr6:55168534-55168553 MsG0680033028.01.T01:CDS 40.0%
AACATGCCCTTCACAAATGG+TGG + Chr6:55169325-55169344 None:intergenic 45.0%
AAGCCAGTAACGGTATCTGA+GGG - Chr6:55168386-55168405 MsG0680033028.01.T01:three_prime_UTR 45.0%
AGTAACGGTATCTGAGGGAT+CGG - Chr6:55168391-55168410 MsG0680033028.01.T01:three_prime_UTR 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG - Chr6:55170540-55170559 MsG0680033028.01.T01:CDS 45.0%
CCGCCACAGCAAATTTATGA+AGG + Chr6:55169268-55169287 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG - Chr6:55170484-55170503 MsG0680033028.01.T01:CDS 45.0%
CTCTCAAAGTCATCCCTACA+AGG + Chr6:55170103-55170122 None:intergenic 45.0%
CTTGTAGGGATGACTTTGAG+AGG - Chr6:55170101-55170120 MsG0680033028.01.T01:intron 45.0%
GATTCAAGAACGACACAACC+AGG + Chr6:55168617-55168636 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+TGG + Chr6:55169120-55169139 None:intergenic 45.0%
GGACATACGAAGTTCGAGAA+GGG - Chr6:55170477-55170496 MsG0680033028.01.T01:CDS 45.0%
GGATCACCGCAGAAAATTGT+AGG + Chr6:55169128-55169147 None:intergenic 45.0%
GGCACTATAGTGCTATCACA+TGG - Chr6:55169485-55169504 MsG0680033028.01.T01:intron 45.0%
GGTCGGAATCAGAATTTGGT+GGG + Chr6:55169159-55169178 None:intergenic 45.0%
TAGCTCCTACTCCTGAATTG+TGG - Chr6:55168513-55168532 MsG0680033028.01.T01:exon 45.0%
TCCTTGAAATTCCGGGTTCA+CGG + Chr6:55169067-55169086 None:intergenic 45.0%
TCTGTTATGGCTAGACCATG+CGG - Chr6:55170611-55170630 MsG0680033028.01.T01:CDS 45.0%
TGAGTCCACTATGAAAACCG+TGG - Chr6:55169518-55169537 MsG0680033028.01.T01:intron 45.0%
TGGTCGGAATCAGAATTTGG+TGG + Chr6:55169160-55169179 None:intergenic 45.0%
TTTCGCTATCAGGACAGTAG+CGG - Chr6:55169552-55169571 MsG0680033028.01.T01:intron 45.0%
! ACAATTTTCTGCGGTGATCC+TGG - Chr6:55169128-55169147 MsG0680033028.01.T01:intron 45.0%
! ACTGTCCTGATAGCGAAACT+GGG + Chr6:55169550-55169569 None:intergenic 45.0%
! AGAGGAAAATCGGGAAGTAC+CGG + Chr6:55170352-55170371 None:intergenic 45.0%
! AGGGATGACTTTGAGAGGAT+TGG - Chr6:55170106-55170125 MsG0680033028.01.T01:intron 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG - Chr6:55168617-55168636 MsG0680033028.01.T01:CDS 45.0%
! GGTTTTCCTGAAGAGTTTGC+TGG - Chr6:55170175-55170194 MsG0680033028.01.T01:intron 45.0%
! TACTGTCCTGATAGCGAAAC+TGG + Chr6:55169551-55169570 None:intergenic 45.0%
! TTGAATCTGGCACTGGAAGT+GGG - Chr6:55168630-55168649 MsG0680033028.01.T01:CDS 45.0%
!! GACGGTAATGATGGCTCTAA+TGG - Chr6:55170521-55170540 MsG0680033028.01.T01:CDS 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG - Chr6:55169234-55169253 MsG0680033028.01.T01:intron 45.0%
!!! GCTTGGCTGATGCAGTTTTT+TGG - Chr6:55170197-55170216 MsG0680033028.01.T01:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG - Chr6:55170456-55170475 MsG0680033028.01.T01:intron 45.0%
AAATTCCGGGTTCACGGGTT+GGG + Chr6:55169061-55169080 None:intergenic 50.0%
AAGAGGAGGCAACGTTCAGA+TGG - Chr6:55170557-55170576 MsG0680033028.01.T01:CDS 50.0%
ACCATACTCCAGCATGCCAA+GGG - Chr6:55169176-55169195 MsG0680033028.01.T01:intron 50.0%
AGTGAGGGACATTCAAGGTG+AGG - Chr6:55170153-55170172 MsG0680033028.01.T01:intron 50.0%
CCTTGAAATTCCGGGTTCAC+GGG + Chr6:55169066-55169085 None:intergenic 50.0%
CGGACATACGAAGTTCGAGA+AGG - Chr6:55170476-55170495 MsG0680033028.01.T01:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG + Chr6:55170475-55170494 None:intergenic 50.0%
GAAGCCAGTAACGGTATCTG+AGG - Chr6:55168385-55168404 MsG0680033028.01.T01:three_prime_UTR 50.0%
GATCCCTCAGATACCGTTAC+TGG + Chr6:55168392-55168411 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG - Chr6:55169354-55169373 MsG0680033028.01.T01:intron 50.0%
GCTATCCCAGTTTCGCTATC+AGG - Chr6:55169542-55169561 MsG0680033028.01.T01:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG + Chr6:55170234-55170253 None:intergenic 50.0%
GGAATAGCTAGCCAAGGTTG+AGG + Chr6:55170228-55170247 None:intergenic 50.0%
GTCGTTCTTGAATCTGGCAC+TGG - Chr6:55168623-55168642 MsG0680033028.01.T01:CDS 50.0%
GTGAGGGACATTCAAGGTGA+GGG - Chr6:55170154-55170173 MsG0680033028.01.T01:intron 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG - Chr6:55170543-55170562 MsG0680033028.01.T01:CDS 50.0%
TTGGCATGCTGGAGTATGGT+CGG + Chr6:55169176-55169195 None:intergenic 50.0%
TTTGATACCCCTTGGCATGC+TGG + Chr6:55169187-55169206 None:intergenic 50.0%
! CTTGAATCTGGCACTGGAAG+TGG - Chr6:55168629-55168648 MsG0680033028.01.T01:CDS 50.0%
! TAGCGCCACGGTTTTCATAG+TGG + Chr6:55169526-55169545 None:intergenic 50.0%
! TTTGACTTCCTTGGCCATGG+CGG - Chr6:55169237-55169256 MsG0680033028.01.T01:intron 50.0%
!! TCCTGAAGAGTTTGCTGGCT+TGG - Chr6:55170180-55170199 MsG0680033028.01.T01:intron 50.0%
!! TTAGGCGGTGTTGTCAATGG+TGG - Chr6:55168961-55168980 MsG0680033028.01.T01:intron 50.0%
ACCGTGGCCACCATTTGTGA+AGG - Chr6:55169315-55169334 MsG0680033028.01.T01:intron 55.0%
ACGTTCAGATGGAGCTAGCG+TGG - Chr6:55170568-55170587 MsG0680033028.01.T01:CDS 55.0%
ACTTAGTCACTGCGGGAGTG+AGG - Chr6:55170137-55170156 MsG0680033028.01.T01:intron 55.0%
AGACCATGCGGTGAAGCTAG+AGG - Chr6:55170623-55170642 MsG0680033028.01.T01:CDS 55.0%
ATCTGCATGTGTGCCCAACG+TGG - Chr6:55169658-55169677 MsG0680033028.01.T01:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG - Chr6:55169177-55169196 MsG0680033028.01.T01:intron 55.0%
CCCGTGAACCCGGAATTTCA+AGG - Chr6:55169063-55169082 MsG0680033028.01.T01:CDS 55.0%
CCCTTCACAAATGGTGGCCA+CGG + Chr6:55169319-55169338 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG - Chr6:55169316-55169335 MsG0680033028.01.T01:intron 55.0%
CTGATCCAGCCCTATGCTCA+TGG + Chr6:55169362-55169381 None:intergenic 55.0%
CTTAGTCACTGCGGGAGTGA+GGG - Chr6:55170138-55170157 MsG0680033028.01.T01:intron 55.0%
GAAATTCCGGGTTCACGGGT+TGG + Chr6:55169062-55169081 None:intergenic 55.0%
GAACGTTGCCTCCTCTTGCA+AGG + Chr6:55170554-55170573 None:intergenic 55.0%
GACCATACTCCAGCATGCCA+AGG - Chr6:55169175-55169194 MsG0680033028.01.T01:intron 55.0%
GCCAAGCCAGCAAACTCTTC+AGG + Chr6:55170184-55170203 None:intergenic 55.0%
GGTGAAGCTAGAGGTCACAC+TGG - Chr6:55170632-55170651 MsG0680033028.01.T01:CDS 55.0%
TCTGCCTCACTATTCCACCG+TGG - Chr6:55169299-55169318 MsG0680033028.01.T01:intron 55.0%
TTATGAAGGGATGCCCGCCA+TGG + Chr6:55169254-55169273 None:intergenic 55.0%
TTCACAAATGGTGGCCACGG+TGG + Chr6:55169316-55169335 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+GGG - Chr6:55169238-55169257 MsG0680033028.01.T01:intron 55.0%
! AGAATTTGGTGGGTGCAGCC+AGG + Chr6:55169149-55169168 None:intergenic 55.0%
! TGACCTCTAGCTTCACCGCA+TGG + Chr6:55170629-55170648 None:intergenic 55.0%
!! GCGGTGTTGTCAATGGTGGA+AGG - Chr6:55168965-55168984 MsG0680033028.01.T01:intron 55.0%
!! TCGGGAAGTACCGGTGAAGT+TGG + Chr6:55170343-55170362 None:intergenic 55.0%
AGTCAGGTGCAGCACCTAGC+TGG + Chr6:55169400-55169419 None:intergenic 60.0%
ATGGACCCAACCCGTGAACC+CGG - Chr6:55169053-55169072 MsG0680033028.01.T01:CDS 60.0%
CCCCTTGGCATGCTGGAGTA+TGG + Chr6:55169180-55169199 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG - Chr6:55168671-55168690 MsG0680033028.01.T01:CDS 60.0%
GCGAAACTGGGATAGCGCCA+CGG + Chr6:55169538-55169557 None:intergenic 60.0%
GCGGGAGTGAGGGACATTCA+AGG - Chr6:55170148-55170167 MsG0680033028.01.T01:intron 60.0%
GTGGCCACGGTGGAATAGTG+AGG + Chr6:55169306-55169325 None:intergenic 60.0%
AGGGATGCCCGCCATGGCCA+AGG + Chr6:55169248-55169267 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 55168276 55170934 55168276 ID=MsG0680033028.01;Name=MsG0680033028.01
Chr6 mRNA 55168276 55170934 55168276 ID=MsG0680033028.01.T01;Parent=MsG0680033028.01;Name=MsG0680033028.01.T01;_AED=0.39;_eAED=0.39;_QI=17|0.5|0.66|1|1|1|3|252|247
Chr6 exon 55170469 55170934 55170469 ID=MsG0680033028.01.T01:exon:9038;Parent=MsG0680033028.01.T01
Chr6 exon 55169041 55169102 55169041 ID=MsG0680033028.01.T01:exon:9039;Parent=MsG0680033028.01.T01
Chr6 exon 55168276 55168760 55168276 ID=MsG0680033028.01.T01:exon:9040;Parent=MsG0680033028.01.T01
Chr6 five_prime_UTR 55170918 55170934 55170918 ID=MsG0680033028.01.T01:five_prime_utr;Parent=MsG0680033028.01.T01
Chr6 CDS 55170469 55170917 55170469 ID=MsG0680033028.01.T01:cds;Parent=MsG0680033028.01.T01
Chr6 CDS 55169041 55169102 55169041 ID=MsG0680033028.01.T01:cds;Parent=MsG0680033028.01.T01
Chr6 CDS 55168528 55168760 55168528 ID=MsG0680033028.01.T01:cds;Parent=MsG0680033028.01.T01
Chr6 three_prime_UTR 55168276 55168527 55168276 ID=MsG0680033028.01.T01:three_prime_utr;Parent=MsG0680033028.01.T01
Gene Sequence

>MsG0680033028.01.T01

ATGTTGGCTATCGATTCTACAAGAAAGATATCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGAAAGGCATGACATCATGAAGCCAGTAACGGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTTAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAACAATAAGGGCGGTTTTGTTTACTTATTAGCTCCTACTCCTGAATTGTGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATATTGCAGATATCAGCTTCATTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGCACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGGATGACTTTGAGAGGATTGGTATAAGCAACTTAGTCACTGCGGGAGTGAGGGACATTCAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTCAGAGATTGACGGTAATGATGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAACGTTCAGATGGAGCTAGCGTGGTGAGCTATCCCAATTCTTCTTCTGTTATGGCTAGACCATGCGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>MsG0680033028.01.T01

MLAIDSTRKISFNRTISNGDLAIVYERHDIMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFNNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFERIGISNLVTAGVRDIQDIRTFEVLLRTYEVREGKMESSEIDGNDGSNGSLPCKRRQRSDGASVVSYPNSSSVMARPCGEARGHTGYLTFARLN*