AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033029.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033029.01.T01 MTR_5g091440 66.154 130 11 1 17 146 3 99 2.78e-51 160
MsG0680033029.01.T01 MTR_8g088220 83.784 74 12 0 19 92 4 77 1.76e-40 131
MsG0680033029.01.T01 MTR_1g081930 66.292 89 30 0 13 101 42 130 1.24e-35 120
MsG0680033029.01.T01 MTR_7g063280 50.000 100 50 0 12 111 75 174 3.52e-32 113
MsG0680033029.01.T01 MTR_4g010930 86.207 58 8 0 50 107 1 58 4.57e-29 104
MsG0680033029.01.T01 MTR_6g016265 46.809 94 50 0 2 95 32 125 8.76e-26 95.5
MsG0680033029.01.T01 MTR_2g027020 38.462 143 84 1 4 146 23 161 6.73e-24 92.4
MsG0680033029.01.T01 MTR_7g013520 37.037 135 60 1 12 146 73 182 1.46e-22 89.0
MsG0680033029.01.T01 MTR_1g088650 41.667 108 63 0 4 111 81 188 2.45e-22 88.2
MsG0680033029.01.T01 MTR_7g082560 39.844 128 46 1 13 140 10 106 2.67e-22 85.9
MsG0680033029.01.T01 MTR_8g038340 70.909 55 16 0 12 66 192 246 1.41e-21 87.8
MsG0680033029.01.T01 MTR_5g049680 56.250 64 28 0 12 75 34 97 1.94e-21 83.6
MsG0680033029.01.T01 MTR_6g034915 39.850 133 63 3 13 145 59 174 5.58e-21 84.3
MsG0680033029.01.T01 MTR_0015s0120 57.746 71 30 0 12 82 296 366 9.77e-21 87.0
MsG0680033029.01.T01 MTR_5g075610 42.424 99 55 1 15 113 1 97 4.51e-20 80.1
MsG0680033029.01.T01 MTR_8g098925 43.617 94 53 0 13 106 69 162 4.67e-20 81.6
MsG0680033029.01.T01 MTR_6g089560 33.333 135 68 1 12 146 119 231 4.88e-20 83.2
MsG0680033029.01.T01 MTR_0093s0070 47.500 80 42 0 12 91 126 205 5.52e-20 82.4
MsG0680033029.01.T01 MTR_1g045560 35.965 114 49 2 15 124 1 94 6.88e-20 79.3
MsG0680033029.01.T01 MTR_3g116210 43.902 82 46 0 12 93 100 181 1.15e-19 81.3
MsG0680033029.01.T01 MTR_1g074090 57.812 64 27 0 12 75 84 147 1.76e-19 80.1
MsG0680033029.01.T01 MTR_4g008270 46.753 77 41 0 12 88 13 89 2.49e-19 77.8
MsG0680033029.01.T01 MTR_5g034450 59.016 61 25 0 12 72 52 112 3.00e-19 78.2
MsG0680033029.01.T01 MTR_8g059135 43.956 91 51 0 12 102 31 121 3.34e-19 78.6
MsG0680033029.01.T01 MTR_2g059470 43.373 83 47 0 10 92 1 83 6.00e-19 76.6
MsG0680033029.01.T01 MTR_7g053400 61.017 59 23 0 13 71 179 237 7.51e-19 80.5
MsG0680033029.01.T01 MTR_5g036320 52.113 71 34 0 4 74 74 144 1.02e-18 77.8
MsG0680033029.01.T01 MTR_5g069700 50.746 67 33 0 30 96 2 68 2.02e-18 75.1
MsG0680033029.01.T01 MTR_6g453270 45.882 85 46 0 4 88 295 379 2.42e-18 80.9
MsG0680033029.01.T01 MTR_8g036780 58.929 56 23 0 12 67 37 92 3.34e-18 75.5
MsG0680033029.01.T01 MTR_1g069475 43.529 85 48 0 4 88 159 243 4.82e-18 78.6
MsG0680033029.01.T01 MTR_1g050488 50.725 69 34 0 4 72 93 161 1.30e-17 75.5
MsG0680033029.01.T01 MTR_5g083200 42.857 91 52 0 3 93 92 182 1.70e-17 75.9
MsG0680033029.01.T01 MTR_0002s1220 42.857 91 52 0 3 93 92 182 1.70e-17 75.9
MsG0680033029.01.T01 MTR_6g016135 63.333 60 22 0 13 72 383 442 3.12e-17 77.8
MsG0680033029.01.T01 MTR_1g017840 40.449 89 53 0 4 92 73 161 4.12e-17 73.9
MsG0680033029.01.T01 MTR_3g111390 42.857 91 52 0 3 93 92 182 5.14e-17 74.7
MsG0680033029.01.T01 MTR_1g070820 42.857 91 52 0 3 93 92 182 5.14e-17 74.7
MsG0680033029.01.T01 MTR_1g045340 41.758 91 53 0 3 93 78 168 7.09e-17 73.9
MsG0680033029.01.T01 MTR_2g055490 34.586 133 54 1 14 146 12 111 1.39e-16 71.2
MsG0680033029.01.T01 MTR_1g074240 53.226 62 29 0 12 73 3 64 1.42e-16 70.5
MsG0680033029.01.T01 MTR_1g034430 38.043 92 57 0 4 95 90 181 8.38e-16 71.2
MsG0680033029.01.T01 MTR_5g037920 36.508 126 76 3 4 127 93 216 1.21e-15 71.6
MsG0680033029.01.T01 MTR_8g099580 46.250 80 43 0 13 92 210 289 1.21e-15 72.8
MsG0680033029.01.T01 MTR_5g085210 36.364 110 54 1 12 121 122 215 2.44e-15 70.9
MsG0680033029.01.T01 MTR_4g073820 52.632 76 26 3 15 90 1 66 2.93e-15 67.0
MsG0680033029.01.T01 MTR_7g077900 51.471 68 33 0 83 150 64 131 4.43e-15 68.6
MsG0680033029.01.T01 MTR_8g031800 50.877 57 28 0 12 68 34 90 6.05e-15 67.0
MsG0680033029.01.T01 MTR_6g016355 51.667 60 29 0 12 71 119 178 8.99e-15 68.6
MsG0680033029.01.T01 MTR_4g064590 33.621 116 71 2 33 146 50 161 1.71e-14 67.4
MsG0680033029.01.T01 MTR_5g031620 42.169 83 48 0 64 146 139 221 2.16e-14 68.2
MsG0680033029.01.T01 MTR_4g006490 62.745 51 19 0 11 61 30 80 3.01e-14 64.7
MsG0680033029.01.T01 MTR_3g010950 30.370 135 64 2 12 146 29 133 4.34e-14 65.5
MsG0680033029.01.T01 MTR_3g026270 48.077 52 27 0 17 68 3 54 1.28e-13 62.4
MsG0680033029.01.T01 MTR_7g072030 60.870 46 18 0 29 74 69 114 2.19e-13 63.5
MsG0680033029.01.T01 MTR_5g047250 33.333 132 61 1 16 147 4 108 2.32e-13 62.8
MsG0680033029.01.T01 MTR_1g109000 55.000 60 27 0 10 69 27 86 5.69e-13 61.2
MsG0680033029.01.T01 MTR_3g090920 33.333 120 64 2 27 146 5 108 7.03e-13 61.6
MsG0680033029.01.T01 MTR_7g006380 32.500 120 54 1 4 123 61 153 8.02e-13 63.2
MsG0680033029.01.T01 MTR_8g079540 48.387 62 32 0 12 73 51 112 1.36e-12 61.2
MsG0680033029.01.T01 MTR_4g029360 37.755 98 61 0 12 109 130 227 1.38e-12 63.5
MsG0680033029.01.T01 MTR_1g017820 50.000 52 26 0 27 78 18 69 1.47e-12 60.1
MsG0680033029.01.T01 MTR_2g047100 37.113 97 46 1 12 108 21 102 2.07e-12 60.5
MsG0680033029.01.T01 MTR_4g094272 54.717 53 23 1 4 56 89 140 1.48e-11 58.9
MsG0680033029.01.T01 MTR_6g013970 36.486 74 47 0 17 90 107 180 1.68e-11 60.1
MsG0680033029.01.T01 MTR_8g028785 44.304 79 44 0 38 116 287 365 3.08e-11 60.5
MsG0680033029.01.T01 MTR_1g062840 38.889 72 44 0 33 104 4 75 3.89e-11 56.6
MsG0680033029.01.T01 MTR_1g022215 41.270 63 37 0 3 65 8 70 5.59e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCTCCTTGATGTGGATTT+GGG 0.212814 6:-55177435 MsG0680033029.01.T01:CDS
TCCGCTCCTTGATGTGGATT+TGG 0.217144 6:-55177436 MsG0680033029.01.T01:CDS
CTAGTTTCCATATTCATATT+AGG 0.250520 6:-55177122 MsG0680033029.01.T01:CDS
TTGGCATTGGTGTGTGTATT+CGG 0.282005 6:-55177503 MsG0680033029.01.T01:CDS
CGGAGTCCTCCATTCAGTTT+TGG 0.287528 6:+55177455 None:intergenic
TTTATTATCTGCAATGTATT+GGG 0.311518 6:-55177364 MsG0680033029.01.T01:CDS
ATGGAATCCTAATATGAATA+TGG 0.311930 6:+55177115 None:intergenic
ATTTAGTAACCTCTGATGTT+AGG 0.318159 6:-55177206 MsG0680033029.01.T01:CDS
AATGTATTGGGTGAGGCTTT+AGG 0.327764 6:-55177387 MsG0680033029.01.T01:intron
TCTTCTTGTCATAAAATAGA+AGG 0.331263 6:-55177051 MsG0680033029.01.T01:CDS
GTTTATTATCTGCAATGTAT+TGG 0.344585 6:-55177365 MsG0680033029.01.T01:CDS
ACTGTAGCGGATAGTCTTTA+TGG 0.356870 6:-55177294 MsG0680033029.01.T01:CDS
TTGGGTTCGTGATCTGCAAC+TGG 0.365664 6:-55177346 MsG0680033029.01.T01:CDS
TGGCATTGGTGTGTGTATTC+GGG 0.392834 6:-55177502 MsG0680033029.01.T01:CDS
TTATGACAAGAAGAAACTAA+TGG 0.477251 6:+55177060 None:intergenic
AAGTCGCTCATAGATTTGCT+AGG 0.541696 6:-55177161 MsG0680033029.01.T01:CDS
GAGGACTCCGCTCCTTGATG+TGG 0.556770 6:-55177442 MsG0680033029.01.T01:CDS
CGCTCATAGATTTGCTAGGG+TGG 0.560211 6:-55177157 MsG0680033029.01.T01:CDS
CGCTCCTTGATGTGGATTTG+GGG 0.560601 6:-55177434 MsG0680033029.01.T01:intron
TGATCTGCAACTGGGTATTG+TGG 0.564013 6:-55177337 MsG0680033029.01.T01:CDS
AGTCTTTATGGTAGTAAATG+TGG 0.567432 6:-55177282 MsG0680033029.01.T01:CDS
CTAACAGACGTCTACAGTCT+CGG 0.571708 6:+55177235 None:intergenic
ATAATAGTAGAGATACAAGA+TGG 0.594546 6:+55177096 None:intergenic
CTCACCCCAAATCCACATCA+AGG 0.600168 6:+55177430 None:intergenic
TGGGTTCGTGATCTGCAACT+GGG 0.604990 6:-55177345 MsG0680033029.01.T01:CDS
AGTCGCTCATAGATTTGCTA+GGG 0.611530 6:-55177160 MsG0680033029.01.T01:CDS
AAATCTATGAGCGACTTCGA+TGG 0.612457 6:+55177167 None:intergenic
TCGAATAAACCTAACATCAG+AGG 0.620069 6:+55177197 None:intergenic
GTGTGTATTCGGGATGATGA+AGG 0.620128 6:-55177492 MsG0680033029.01.T01:CDS
CCCAAATCCACATCAAGGAG+CGG 0.647777 6:+55177435 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAACTGCTAAAATAATTTT+TGG - Chr6:55177059-55177078 MsG0680033029.01.T01:CDS 15.0%
!! TTTATTATCTGCAATGTATT+GGG - Chr6:55177182-55177201 MsG0680033029.01.T01:CDS 20.0%
! ATAATAGTAGAGATACAAGA+TGG + Chr6:55177488-55177507 None:intergenic 25.0%
! ATGGAATCCTAATATGAATA+TGG + Chr6:55177469-55177488 None:intergenic 25.0%
! CTAGTTTCCATATTCATATT+AGG - Chr6:55177459-55177478 MsG0680033029.01.T01:CDS 25.0%
! GTTTATTATCTGCAATGTAT+TGG - Chr6:55177181-55177200 MsG0680033029.01.T01:CDS 25.0%
! TCTTCTTGTCATAAAATAGA+AGG - Chr6:55177530-55177549 MsG0680033029.01.T01:CDS 25.0%
! TTATGACAAGAAGAAACTAA+TGG + Chr6:55177524-55177543 None:intergenic 25.0%
!!! GCTAAAATAATTTTTGGCAT+TGG - Chr6:55177065-55177084 MsG0680033029.01.T01:CDS 25.0%
AGTCTTTATGGTAGTAAATG+TGG - Chr6:55177299-55177318 MsG0680033029.01.T01:CDS 30.0%
ATTTAGTAACCTCTGATGTT+AGG - Chr6:55177375-55177394 MsG0680033029.01.T01:CDS 30.0%
!!! ATGTTTTAGCCAAAACTGAA+TGG - Chr6:55177117-55177136 MsG0680033029.01.T01:CDS 30.0%
GCTTGATTCAAAAACTGTAG+CGG - Chr6:55177274-55177293 MsG0680033029.01.T01:CDS 35.0%
TCGAATAAACCTAACATCAG+AGG + Chr6:55177387-55177406 None:intergenic 35.0%
! TTTTAGCCAAAACTGAATGG+AGG - Chr6:55177120-55177139 MsG0680033029.01.T01:CDS 35.0%
AAATCTATGAGCGACTTCGA+TGG + Chr6:55177417-55177436 None:intergenic 40.0%
AATGTATTGGGTGAGGCTTT+AGG - Chr6:55177194-55177213 MsG0680033029.01.T01:CDS 40.0%
TATCTGCAATGTATTGGGTG+AGG - Chr6:55177187-55177206 MsG0680033029.01.T01:CDS 40.0%
! AAGTCGCTCATAGATTTGCT+AGG - Chr6:55177420-55177439 MsG0680033029.01.T01:intron 40.0%
!! ACTGTAGCGGATAGTCTTTA+TGG - Chr6:55177287-55177306 MsG0680033029.01.T01:CDS 40.0%
!! AGTCGCTCATAGATTTGCTA+GGG - Chr6:55177421-55177440 MsG0680033029.01.T01:intron 40.0%
!! TTGGCATTGGTGTGTGTATT+CGG - Chr6:55177078-55177097 MsG0680033029.01.T01:CDS 40.0%
CTAACAGACGTCTACAGTCT+CGG + Chr6:55177349-55177368 None:intergenic 45.0%
GTGTGTATTCGGGATGATGA+AGG - Chr6:55177089-55177108 MsG0680033029.01.T01:CDS 45.0%
! TGATCTGCAACTGGGTATTG+TGG - Chr6:55177244-55177263 MsG0680033029.01.T01:CDS 45.0%
!! TGGCATTGGTGTGTGTATTC+GGG - Chr6:55177079-55177098 MsG0680033029.01.T01:CDS 45.0%
CCCAAATCCACATCAAGGAG+CGG + Chr6:55177149-55177168 None:intergenic 50.0%
CCGCTCCTTGATGTGGATTT+GGG - Chr6:55177146-55177165 MsG0680033029.01.T01:CDS 50.0%
CGCTCCTTGATGTGGATTTG+GGG - Chr6:55177147-55177166 MsG0680033029.01.T01:CDS 50.0%
CTCACCCCAAATCCACATCA+AGG + Chr6:55177154-55177173 None:intergenic 50.0%
TCCGCTCCTTGATGTGGATT+TGG - Chr6:55177145-55177164 MsG0680033029.01.T01:CDS 50.0%
TGGGTTCGTGATCTGCAACT+GGG - Chr6:55177236-55177255 MsG0680033029.01.T01:CDS 50.0%
TTGGGTTCGTGATCTGCAAC+TGG - Chr6:55177235-55177254 MsG0680033029.01.T01:CDS 50.0%
! CGGAGTCCTCCATTCAGTTT+TGG + Chr6:55177129-55177148 None:intergenic 50.0%
! CTTGATGTGGATTTGGGGTG+AGG - Chr6:55177152-55177171 MsG0680033029.01.T01:CDS 50.0%
! TGGATTTGGGGTGAGGCTTT+AGG - Chr6:55177159-55177178 MsG0680033029.01.T01:CDS 50.0%
!! CGCTCATAGATTTGCTAGGG+TGG - Chr6:55177424-55177443 MsG0680033029.01.T01:intron 50.0%
GAGGACTCCGCTCCTTGATG+TGG - Chr6:55177139-55177158 MsG0680033029.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 55177047 55177556 55177047 ID=MsG0680033029.01;Name=MsG0680033029.01
Chr6 mRNA 55177047 55177556 55177047 ID=MsG0680033029.01.T01;Parent=MsG0680033029.01;Name=MsG0680033029.01.T01;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|0|0|2|0|157
Chr6 exon 55177435 55177556 55177435 ID=MsG0680033029.01.T01:exon:9069;Parent=MsG0680033029.01.T01
Chr6 exon 55177047 55177398 55177047 ID=MsG0680033029.01.T01:exon:9070;Parent=MsG0680033029.01.T01
Chr6 CDS 55177435 55177556 55177435 ID=MsG0680033029.01.T01:cds;Parent=MsG0680033029.01.T01
Chr6 CDS 55177047 55177398 55177047 ID=MsG0680033029.01.T01:cds;Parent=MsG0680033029.01.T01
Gene Sequence

>MsG0680033029.01.T01

ATGACAAAATTCAAAACTGCTAAAATAATTTTTGGCATTGGTGTGTGTATTCGGGATGATGAAGGTCGTTATGTTTTAGCCAAAACTGAATGGAGGACTCCGCTCCTTGATGTGGATTTGGGTGAGGCTTTAGGTTTATTATCTGCAATGTATTGGGTTCGTGATCTGCAACTGGGTATTGTGGACTTTGAGCTTGATTCAAAAACTGTAGCGGATAGTCTTTATGGTAGTAAATGTGGCGTCTCTAATTTTAGCGCAGTGATCCGAGACTGTAGACGTCTGTTAGCTTCTGATTTAGTAACCTCTGATGTTAGGTTTATTCGAAGACAAGCCATCGAAGTCGCTCATAGATTTGCTAGGGTGGCTTCGCGTCATGCTAGTTTCCATATTCATATTAGGATTCCATCTTGTATCTCTACTATTATTATGAATGAAATGCCATTAGTTTCTTCTTGTCATAAAATAGAAGGATGA

Protein sequence

>MsG0680033029.01.T01

MTKFKTAKIIFGIGVCIRDDEGRYVLAKTEWRTPLLDVDLGEALGLLSAMYWVRDLQLGIVDFELDSKTVADSLYGSKCGVSNFSAVIRDCRRLLASDLVTSDVRFIRRQAIEVAHRFARVASRHASFHIHIRIPSCISTIIMNEMPLVSSCHKIEG*