AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033584.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033584.01.T01 MTR_4g015450 80.935 278 52 1 13 289 195 472 8.22e-167 471
MsG0680033584.01.T01 MTR_4g015410 76.817 289 43 1 1 289 107 371 5.65e-157 442
MsG0680033584.01.T01 MTR_1g040690 64.948 291 97 4 1 287 182 471 4.57e-130 378
MsG0680033584.01.T01 MTR_1g038270 48.864 264 115 6 24 279 246 497 3.64e-75 238
MsG0680033584.01.T01 MTR_7g068160 28.800 375 156 13 1 287 178 529 3.02e-29 116
MsG0680033584.01.T01 MTR_4g015290 75.000 68 17 0 18 85 156 223 1.65e-26 104
MsG0680033584.01.T01 MTR_7g067300 28.363 342 154 14 1 287 166 471 2.59e-25 105
MsG0680033584.01.T01 MTR_3g466740 30.400 250 137 8 1 216 167 413 3.30e-25 104
MsG0680033584.01.T01 MTR_7g067970 27.704 379 161 13 1 287 173 530 4.83e-25 104
MsG0680033584.01.T01 MTR_0067s0010 27.864 323 168 13 1 287 180 473 1.66e-23 100
MsG0680033584.01.T01 MTR_7g067600 29.433 282 124 7 1 207 174 455 2.58e-23 99.8
MsG0680033584.01.T01 MTR_7g067940 27.108 332 139 11 1 230 164 494 6.32e-23 98.6
MsG0680033584.01.T01 MTR_7g067750 26.536 358 171 9 1 287 205 541 2.46e-22 97.1
MsG0680033584.01.T01 MTR_7g068070 32.569 218 105 6 88 284 338 534 3.12e-22 96.7
MsG0680033584.01.T01 MTR_7g067880 25.824 364 164 16 1 281 171 511 3.88e-21 93.6
MsG0680033584.01.T01 MTR_7g067990 26.611 357 171 10 1 287 254 589 6.60e-20 90.1
MsG0680033584.01.T01 MTR_7g067660 31.556 225 104 10 82 278 174 376 1.49e-18 85.1
MsG0680033584.01.T01 MTR_6g010890 24.756 307 178 9 1 289 155 426 5.80e-17 80.9
MsG0680033584.01.T01 MTR_5g086340 26.557 305 167 10 1 289 170 433 9.71e-17 80.5
MsG0680033584.01.T01 MTR_6g010850 25.000 296 168 10 1 283 89 343 9.74e-16 77.0
MsG0680033584.01.T01 MTR_6g011060 24.675 308 172 10 1 287 149 417 3.30e-15 75.9
MsG0680033584.01.T01 MTR_6g010920 25.000 300 175 9 1 289 149 409 5.65e-15 75.1
MsG0680033584.01.T01 MTR_3g107590 27.273 242 150 9 3 229 160 390 1.11e-13 71.2
MsG0680033584.01.T01 MTR_6g009590 27.511 229 142 7 1 216 149 366 1.84e-13 70.5
MsG0680033584.01.T01 MTR_2g035640 23.490 298 165 11 1 284 96 344 4.56e-12 66.2
MsG0680033584.01.T01 MTR_2g041180 29.412 221 133 6 1 206 181 393 1.36e-11 65.1
MsG0680033584.01.T01 MTR_8g006525 28.452 239 146 7 1 218 161 395 2.41e-11 64.3
MsG0680033584.01.T01 MTR_6g009610 25.820 244 154 9 1 229 149 380 4.38e-11 63.5
MsG0680033584.01.T01 MTR_3g079390 22.884 319 169 7 1 284 169 445 5.41e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033584.01.T01 AT3G50180 27.419 310 162 13 1 288 312 580 2.30e-16 79.7
MsG0680033584.01.T01 AT3G50150 25.938 320 160 12 1 287 225 500 3.61e-16 79.0
MsG0680033584.01.T01 AT3G50160 26.087 299 165 10 1 287 238 492 1.86e-15 76.6
MsG0680033584.01.T01 AT3G60470 24.348 230 131 3 62 287 343 533 3.38e-15 75.9
MsG0680033584.01.T01 AT3G50170 28.704 216 110 8 75 287 356 530 1.14e-12 68.6
MsG0680033584.01.T01 AT3G50120 23.077 325 173 8 1 287 237 522 1.46e-12 68.2
MsG0680033584.01.T01 AT2G28580 25.581 301 165 12 1 287 191 446 1.90e-12 67.8
MsG0680033584.01.T01 AT2G28580 25.581 301 165 12 1 287 242 497 2.35e-12 67.4
MsG0680033584.01.T01 AT2G28580 25.581 301 165 12 1 287 259 514 2.60e-12 67.4
MsG0680033584.01.T01 AT2G28580 25.914 301 164 13 1 287 256 511 2.81e-12 67.4
MsG0680033584.01.T01 AT2G28580 25.581 301 165 12 1 287 273 528 3.16e-12 67.0
MsG0680033584.01.T01 AT3G50140 25.581 215 120 4 75 288 356 531 1.56e-11 65.1
MsG0680033584.01.T01 AT1G67150 23.154 298 172 9 1 287 150 401 6.05e-11 63.2
MsG0680033584.01.T01 AT1G67150 23.154 298 172 9 1 287 150 401 6.05e-11 63.2
MsG0680033584.01.T01 AT1G67150 23.154 298 172 9 1 287 154 405 6.33e-11 62.8
MsG0680033584.01.T01 AT2G44930 25.418 299 168 9 1 287 239 494 6.42e-11 63.2

Find 52 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGTTTCCCTTCATGTTGA+TGG 0.193667 6:-68853862 None:intergenic
CTTGGGAGCAAAGCAAAATA+TGG 0.290148 6:+68854086 MsG0680033584.01.T01:CDS
TTGTGTACTATGGGAGAAAT+TGG 0.300484 6:+68854404 MsG0680033584.01.T01:CDS
CCTACGGTTTGGAAATTCAT+AGG 0.347519 6:+68854454 MsG0680033584.01.T01:CDS
AGTGGAGGAATTTGATTGCT+TGG 0.350702 6:+68854068 MsG0680033584.01.T01:CDS
CGTGCGTCCGGGGTGATGAT+TGG 0.352720 6:+68853920 MsG0680033584.01.T01:CDS
AATATAGTTCGTAAAGCCTT+TGG 0.372864 6:+68853767 MsG0680033584.01.T01:CDS
AAAATTTGATGCTGAGAAGT+TGG 0.373739 6:+68854006 MsG0680033584.01.T01:CDS
GCTACTGAGCTTCGTGCGTC+CGG 0.379569 6:+68853908 MsG0680033584.01.T01:CDS
ATCCATTTGCTCGACTCCTT+TGG 0.382783 6:-68854257 None:intergenic
TGTCGCAGCTGCTTTGCTCC+TGG 0.391804 6:+68854480 MsG0680033584.01.T01:CDS
TTGTTCTCAAGAAGTTATAT+AGG 0.393685 6:+68853696 MsG0680033584.01.T01:CDS
TCTTTGATTTCACTGCTGTC+TGG 0.399496 6:-68853728 None:intergenic
ATTTCCAAACCGTAGGTATA+TGG 0.411602 6:-68854447 None:intergenic
CCATCAACATGAAGGGAAAC+AGG 0.414642 6:+68853862 MsG0680033584.01.T01:CDS
CTACTGAGCTTCGTGCGTCC+GGG 0.416956 6:+68853909 MsG0680033584.01.T01:CDS
GTGGAGGAATTTGATTGCTT+GGG 0.418003 6:+68854069 MsG0680033584.01.T01:CDS
CCTCCTGAGCTATTCTTTGA+AGG 0.422971 6:-68853794 None:intergenic
CATTGATGCCAAGCATGTAT+AGG 0.425896 6:-68854361 None:intergenic
GTTGTTTCCTTGATATATAG+TGG 0.458518 6:-68854037 None:intergenic
TGCCAAAGGAGTCGAGCAAA+TGG 0.467581 6:+68854255 MsG0680033584.01.T01:CDS
TAACTTCTTGAGAACAATGA+AGG 0.468443 6:-68853690 None:intergenic
GTGACCATATACCTACGGTT+TGG 0.473148 6:+68854443 MsG0680033584.01.T01:CDS
GAAAACAAATTAGGCAATGC+GGG 0.492930 6:-68854533 None:intergenic
TACCCTTCAAAGAATAGCTC+AGG 0.506192 6:+68853791 MsG0680033584.01.T01:CDS
GAAAATTGCCTATACATGCT+TGG 0.522674 6:+68854353 MsG0680033584.01.T01:CDS
AGGAAACAACTGAAGTTAAG+TGG 0.526842 6:+68854050 MsG0680033584.01.T01:CDS
TATTCCGCCACTATATATCA+AGG 0.539545 6:+68854030 MsG0680033584.01.T01:CDS
CTCCTGAGCTATTCTTTGAA+GGG 0.539903 6:-68853793 None:intergenic
GAACAAAACCATCAACATGA+AGG 0.554414 6:+68853854 MsG0680033584.01.T01:CDS
GATACGTGACCATATACCTA+CGG 0.556626 6:+68854438 MsG0680033584.01.T01:CDS
TCTCAAGAAGTTATATAGGA+AGG 0.565626 6:+68853700 MsG0680033584.01.T01:CDS
TACATGCTTGGCATCAATGT+AGG 0.569828 6:+68854365 MsG0680033584.01.T01:CDS
TAAATGTGTTCTACGACAAG+AGG 0.572086 6:+68854308 MsG0680033584.01.T01:CDS
TACTGAGCTTCGTGCGTCCG+GGG 0.574078 6:+68853910 MsG0680033584.01.T01:CDS
TCATCTGATGCATTTGTCAA+CGG 0.583328 6:+68853829 MsG0680033584.01.T01:CDS
TTATTTCGAACGATTGCCAA+AGG 0.584736 6:+68854241 MsG0680033584.01.T01:CDS
TGAGTAATATGTTTGCATGA+TGG 0.596746 6:-68854509 None:intergenic
ATTCTTTGAAGGGTAACCAA+AGG 0.604039 6:-68853783 None:intergenic
AACTTCTTGAGAACAATGAA+GGG 0.607449 6:-68853689 None:intergenic
ACCATTAAAGAAAAGTGCAT+AGG 0.617008 6:-68854128 None:intergenic
AACAAAACCATCAACATGAA+GGG 0.620333 6:+68853855 MsG0680033584.01.T01:CDS
CCTTCAAAGAATAGCTCAGG+AGG 0.621033 6:+68853794 MsG0680033584.01.T01:CDS
TTTGCATGATGGTGAGAACC+AGG 0.622828 6:-68854498 None:intergenic
CCTATGAATTTCCAAACCGT+AGG 0.624886 6:-68854454 None:intergenic
TCTGATGCATTTGTCAACGG+TGG 0.640644 6:+68853832 MsG0680033584.01.T01:CDS
TTTAGCTCCAATCATCACCC+CGG 0.649238 6:-68853927 None:intergenic
AGAAAACAAATTAGGCAATG+CGG 0.658864 6:-68854534 None:intergenic
AAACAACTGAAGTTAAGTGG+AGG 0.666190 6:+68854053 MsG0680033584.01.T01:CDS
AATCAAAGACGATTATCGTG+TGG 0.682935 6:+68853742 MsG0680033584.01.T01:CDS
GTTTCCTTGATATATAGTGG+CGG 0.683204 6:-68854034 None:intergenic
ACATGAAGGGAAACAGGCGA+AGG 0.692968 6:+68853868 MsG0680033584.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAACAGTATAATT+TGG + Chr6:68853961-68853980 MsG0680033584.01.T01:CDS 10.0%
!! TAAAAAACAGTATAATTTGG+TGG + Chr6:68853964-68853983 MsG0680033584.01.T01:CDS 20.0%
!!! TTGAAATTTTTGTGTACTAT+GGG + Chr6:68854395-68854414 MsG0680033584.01.T01:CDS 20.0%
!!! TTTGAAATTTTTGTGTACTA+TGG + Chr6:68854394-68854413 MsG0680033584.01.T01:CDS 20.0%
! ATTGAATTGCTTCAGAAAAA+AGG + Chr6:68854172-68854191 MsG0680033584.01.T01:CDS 25.0%
! TTGAATTGCTTCAGAAAAAA+GGG + Chr6:68854173-68854192 MsG0680033584.01.T01:CDS 25.0%
! TTGTTCTCAAGAAGTTATAT+AGG + Chr6:68853696-68853715 MsG0680033584.01.T01:CDS 25.0%
AAAATTTGATGCTGAGAAGT+TGG + Chr6:68854006-68854025 MsG0680033584.01.T01:CDS 30.0%
AACAAAACCATCAACATGAA+GGG + Chr6:68853855-68853874 MsG0680033584.01.T01:CDS 30.0%
AACTTCTTGAGAACAATGAA+GGG - Chr6:68853692-68853711 None:intergenic 30.0%
AATATAGTTCGTAAAGCCTT+TGG + Chr6:68853767-68853786 MsG0680033584.01.T01:CDS 30.0%
TAACTTCTTGAGAACAATGA+AGG - Chr6:68853693-68853712 None:intergenic 30.0%
TCTCAAGAAGTTATATAGGA+AGG + Chr6:68853700-68853719 MsG0680033584.01.T01:CDS 30.0%
TGAATTGCTTCAGAAAAAAG+GGG + Chr6:68854174-68854193 MsG0680033584.01.T01:CDS 30.0%
TGAGTAATATGTTTGCATGA+TGG - Chr6:68854512-68854531 None:intergenic 30.0%
! ACCATTAAAGAAAAGTGCAT+AGG - Chr6:68854131-68854150 None:intergenic 30.0%
! GTTGTTTCCTTGATATATAG+TGG - Chr6:68854040-68854059 None:intergenic 30.0%
! TCCTATGCACTTTTCTTTAA+TGG + Chr6:68854127-68854146 MsG0680033584.01.T01:CDS 30.0%
AAACAACTGAAGTTAAGTGG+AGG + Chr6:68854053-68854072 MsG0680033584.01.T01:CDS 35.0%
AATCAAAGACGATTATCGTG+TGG + Chr6:68853742-68853761 MsG0680033584.01.T01:CDS 35.0%
AGGAAACAACTGAAGTTAAG+TGG + Chr6:68854050-68854069 MsG0680033584.01.T01:CDS 35.0%
ATTTCCAAACCGTAGGTATA+TGG - Chr6:68854450-68854469 None:intergenic 35.0%
GAAAATTGCCTATACATGCT+TGG + Chr6:68854353-68854372 MsG0680033584.01.T01:CDS 35.0%
GAACAAAACCATCAACATGA+AGG + Chr6:68853854-68853873 MsG0680033584.01.T01:CDS 35.0%
GTTTCCTTGATATATAGTGG+CGG - Chr6:68854037-68854056 None:intergenic 35.0%
TAAATGTGTTCTACGACAAG+AGG + Chr6:68854308-68854327 MsG0680033584.01.T01:CDS 35.0%
TATTCCGCCACTATATATCA+AGG + Chr6:68854030-68854049 MsG0680033584.01.T01:CDS 35.0%
TCATCTGATGCATTTGTCAA+CGG + Chr6:68853829-68853848 MsG0680033584.01.T01:CDS 35.0%
TTATTTCGAACGATTGCCAA+AGG + Chr6:68854241-68854260 MsG0680033584.01.T01:CDS 35.0%
TTGTGTACTATGGGAGAAAT+TGG + Chr6:68854404-68854423 MsG0680033584.01.T01:CDS 35.0%
! ATTCTTTGAAGGGTAACCAA+AGG - Chr6:68853786-68853805 None:intergenic 35.0%
AGTGGAGGAATTTGATTGCT+TGG + Chr6:68854068-68854087 MsG0680033584.01.T01:CDS 40.0%
CATTGATGCCAAGCATGTAT+AGG - Chr6:68854364-68854383 None:intergenic 40.0%
CCTATGAATTTCCAAACCGT+AGG - Chr6:68854457-68854476 None:intergenic 40.0%
CTCCTGAGCTATTCTTTGAA+GGG - Chr6:68853796-68853815 None:intergenic 40.0%
CTTGGGAGCAAAGCAAAATA+TGG + Chr6:68854086-68854105 MsG0680033584.01.T01:CDS 40.0%
GATACGTGACCATATACCTA+CGG + Chr6:68854438-68854457 MsG0680033584.01.T01:CDS 40.0%
GTGGAGGAATTTGATTGCTT+GGG + Chr6:68854069-68854088 MsG0680033584.01.T01:CDS 40.0%
TACATGCTTGGCATCAATGT+AGG + Chr6:68854365-68854384 MsG0680033584.01.T01:CDS 40.0%
TACCCTTCAAAGAATAGCTC+AGG + Chr6:68853791-68853810 MsG0680033584.01.T01:CDS 40.0%
! CCTACGGTTTGGAAATTCAT+AGG + Chr6:68854454-68854473 MsG0680033584.01.T01:CDS 40.0%
! TCTTTGATTTCACTGCTGTC+TGG - Chr6:68853731-68853750 None:intergenic 40.0%
ATCCATTTGCTCGACTCCTT+TGG - Chr6:68854260-68854279 None:intergenic 45.0%
CCATCAACATGAAGGGAAAC+AGG + Chr6:68853862-68853881 MsG0680033584.01.T01:CDS 45.0%
CCTCCTGAGCTATTCTTTGA+AGG - Chr6:68853797-68853816 None:intergenic 45.0%
CCTGTTTCCCTTCATGTTGA+TGG - Chr6:68853865-68853884 None:intergenic 45.0%
CCTTCAAAGAATAGCTCAGG+AGG + Chr6:68853794-68853813 MsG0680033584.01.T01:CDS 45.0%
GTGACCATATACCTACGGTT+TGG + Chr6:68854443-68854462 MsG0680033584.01.T01:CDS 45.0%
TCTGATGCATTTGTCAACGG+TGG + Chr6:68853832-68853851 MsG0680033584.01.T01:CDS 45.0%
! TTTAGCTCCAATCATCACCC+CGG - Chr6:68853930-68853949 None:intergenic 45.0%
! TTTGCATGATGGTGAGAACC+AGG - Chr6:68854501-68854520 None:intergenic 45.0%
ACATGAAGGGAAACAGGCGA+AGG + Chr6:68853868-68853887 MsG0680033584.01.T01:CDS 50.0%
TGCCAAAGGAGTCGAGCAAA+TGG + Chr6:68854255-68854274 MsG0680033584.01.T01:CDS 50.0%
CTACTGAGCTTCGTGCGTCC+GGG + Chr6:68853909-68853928 MsG0680033584.01.T01:CDS 60.0%
GCTACTGAGCTTCGTGCGTC+CGG + Chr6:68853908-68853927 MsG0680033584.01.T01:CDS 60.0%
TACTGAGCTTCGTGCGTCCG+GGG + Chr6:68853910-68853929 MsG0680033584.01.T01:CDS 60.0%
! TGTCGCAGCTGCTTTGCTCC+TGG + Chr6:68854480-68854499 MsG0680033584.01.T01:CDS 60.0%
CGTGCGTCCGGGGTGATGAT+TGG + Chr6:68853920-68853939 MsG0680033584.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 68853671 68854546 68853671 ID=MsG0680033584.01;Name=MsG0680033584.01
Chr6 mRNA 68853671 68854546 68853671 ID=MsG0680033584.01.T01;Parent=MsG0680033584.01;Name=MsG0680033584.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|291
Chr6 exon 68853671 68854546 68853671 ID=MsG0680033584.01.T01:exon:2591;Parent=MsG0680033584.01.T01
Chr6 CDS 68853671 68854546 68853671 ID=MsG0680033584.01.T01:cds;Parent=MsG0680033584.01.T01
Gene Sequence

>MsG0680033584.01.T01

ATGCTTGAGAATCAAATTCCCTTCATTGTTCTCAAGAAGTTATATAGGAAGGTATTTCCAGACAGCAGTGAAATCAAAGACGATTATCGTGTGGCTAATATAGTTCGTAAAGCCTTTGGTTACCCTTCAAAGAATAGCTCAGGAGGTGCTCATATACTTCATCTGATGCATTTGTCAACGGTGGAACAAAACCATCAACATGAAGGGAAACAGGCGAAGGTAGAATTGTTGTGTTGTGCTACTGAGCTTCGTGCGTCCGGGGTGATGATTGGAGCTAAATTAAATAGCACAAATAAAAAACAGTATAATTTGGTGGATATGTTTGATTTTGATATAAAATTTGATGCTGAGAAGTTGGATATTCCGCCACTATATATCAAGGAAACAACTGAAGTTAAGTGGAGGAATTTGATTGCTTGGGAGCAAAGCAAAATATGGATTAGATGCAAATACACGTCCTATGCACTTTTCTTTAATGGTTTGATATGTTGTGAGCATGACATTGAATTGCTTCAGAAAAAAGGGGTTATTGTAAATGAGTTGAACAAAAGCAATGAAGATTTGTTGATATTATTTCGAACGATTGCCAAAGGAGTCGAGCAAATGGATTTGAGTTATAGTGAGATTTGTGAAGAGTTAAATGTGTTCTACGACAAGAGGATGAAAGTCACAAAAGTGTTGCGAAAATTGCCTATACATGCTTGGCATCAATGTAGGCGAATTTTTGAAATTTTTGTGTACTATGGGAGAAATTGGTACAGTGTTTTGATACGTGACCATATACCTACGGTTTGGAAATTCATAGGAATTGTCGCAGCTGCTTTGCTCCTGGTTCTCACCATCATGCAAACATATTACTCATCCCGCATTGCCTAA

Protein sequence

>MsG0680033584.01.T01

MLENQIPFIVLKKLYRKVFPDSSEIKDDYRVANIVRKAFGYPSKNSSGGAHILHLMHLSTVEQNHQHEGKQAKVELLCCATELRASGVMIGAKLNSTNKKQYNLVDMFDFDIKFDAEKLDIPPLYIKETTEVKWRNLIAWEQSKIWIRCKYTSYALFFNGLICCEHDIELLQKKGVIVNELNKSNEDLLILFRTIAKGVEQMDLSYSEICEELNVFYDKRMKVTKVLRKLPIHAWHQCRRIFEIFVYYGRNWYSVLIRDHIPTVWKFIGIVAAALLLVLTIMQTYYSSRIA*