Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033612.01.T01 | XP_013443333.1 | 89.136 | 359 | 21 | 1 | 1 | 359 | 1 | 341 | 0 | 568 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033612.01.T01 | Q9SCW4 | 45.48 | 354 | 101 | 10 | 26 | 358 | 16 | 298 | 1.63E-81 | 253 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033612.01.T01 | A0A072TKJ1 | 89.136 | 359 | 21 | 1 | 1 | 359 | 1 | 341 | 0.0 | 568 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0680033612.01.T01 | TF | HSF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004986.01 | MsG0680033612.01 | 0.800589 | 1.342667e-48 | 6.275832e-46 |
| MsG0680030841.01 | MsG0680033612.01 | 0.801701 | 7.953471e-49 | 3.823444e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033612.01.T01 | MTR_0020s0020 | 89.136 | 359 | 21 | 1 | 1 | 359 | 1 | 341 | 0.0 | 568 |
| MsG0680033612.01.T01 | MTR_1g100777 | 52.342 | 363 | 107 | 11 | 1 | 358 | 1 | 302 | 2.24e-101 | 301 |
| MsG0680033612.01.T01 | MTR_5g010680 | 44.706 | 340 | 105 | 9 | 7 | 304 | 5 | 303 | 4.83e-76 | 239 |
| MsG0680033612.01.T01 | MTR_3g101870 | 41.803 | 244 | 92 | 4 | 23 | 245 | 14 | 228 | 1.01e-54 | 184 |
| MsG0680033612.01.T01 | MTR_5g089170 | 36.111 | 288 | 125 | 8 | 23 | 304 | 15 | 249 | 4.76e-49 | 166 |
| MsG0680033612.01.T01 | MTR_5g017470 | 42.373 | 236 | 80 | 4 | 26 | 248 | 2 | 194 | 1.93e-47 | 162 |
| MsG0680033612.01.T01 | MTR_7g091370 | 39.450 | 218 | 78 | 3 | 27 | 244 | 16 | 179 | 4.95e-40 | 141 |
| MsG0680033612.01.T01 | MTR_6g043060 | 44.138 | 145 | 63 | 1 | 32 | 176 | 21 | 147 | 2.32e-37 | 137 |
| MsG0680033612.01.T01 | MTR_5g029680 | 52.381 | 126 | 42 | 1 | 17 | 142 | 16 | 123 | 8.87e-37 | 139 |
| MsG0680033612.01.T01 | MTR_7g095680 | 44.681 | 141 | 59 | 2 | 3 | 142 | 18 | 140 | 3.02e-36 | 135 |
| MsG0680033612.01.T01 | MTR_8g083100 | 44.218 | 147 | 64 | 1 | 34 | 180 | 23 | 151 | 2.14e-34 | 132 |
| MsG0680033612.01.T01 | MTR_1g071360 | 50.413 | 121 | 38 | 2 | 31 | 151 | 30 | 128 | 2.30e-34 | 129 |
| MsG0680033612.01.T01 | MTR_1g071360 | 50.413 | 121 | 38 | 2 | 31 | 151 | 27 | 125 | 2.94e-34 | 130 |
| MsG0680033612.01.T01 | MTR_4g077970 | 54.545 | 110 | 32 | 1 | 33 | 142 | 12 | 103 | 6.96e-34 | 131 |
| MsG0680033612.01.T01 | MTR_2g100680 | 47.368 | 133 | 52 | 1 | 31 | 163 | 29 | 143 | 3.75e-33 | 128 |
| MsG0680033612.01.T01 | MTR_1g102860 | 45.865 | 133 | 52 | 2 | 12 | 142 | 17 | 131 | 2.03e-32 | 124 |
| MsG0680033612.01.T01 | MTR_1g061170 | 42.208 | 154 | 61 | 3 | 33 | 180 | 49 | 180 | 3.11e-32 | 126 |
| MsG0680033612.01.T01 | MTR_8g087540 | 50.427 | 117 | 39 | 2 | 26 | 142 | 6 | 103 | 6.27e-32 | 124 |
| MsG0680033612.01.T01 | MTR_4g088900 | 32.287 | 223 | 95 | 5 | 31 | 249 | 31 | 201 | 6.75e-32 | 119 |
| MsG0680033612.01.T01 | MTR_3g104550 | 49.107 | 112 | 39 | 1 | 31 | 142 | 44 | 137 | 3.21e-30 | 119 |
| MsG0680033612.01.T01 | MTR_2g100670 | 42.857 | 133 | 58 | 1 | 31 | 163 | 31 | 145 | 3.71e-30 | 117 |
| MsG0680033612.01.T01 | MTR_6g086805 | 48.276 | 116 | 42 | 1 | 27 | 142 | 5 | 102 | 1.62e-29 | 116 |
| MsG0680033612.01.T01 | MTR_2g100670 | 42.857 | 133 | 58 | 1 | 31 | 163 | 139 | 253 | 2.58e-29 | 117 |
| MsG0680033612.01.T01 | MTR_2g017890 | 48.182 | 110 | 39 | 1 | 33 | 142 | 15 | 106 | 4.02e-28 | 113 |
| MsG0680033612.01.T01 | MTR_8g105780 | 50.000 | 112 | 37 | 2 | 32 | 142 | 21 | 114 | 6.97e-27 | 109 |
| MsG0680033612.01.T01 | MTR_4g045757 | 56.897 | 58 | 25 | 0 | 33 | 90 | 14 | 71 | 1.98e-16 | 73.9 |
| MsG0680033612.01.T01 | MTR_5g047190 | 35.577 | 104 | 52 | 2 | 41 | 142 | 1 | 91 | 1.19e-13 | 66.6 |
| MsG0680033612.01.T01 | MTR_4g011850 | 29.630 | 135 | 49 | 2 | 111 | 245 | 9 | 97 | 4.69e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033612.01.T01 | AT5G62020 | 45.480 | 354 | 101 | 10 | 26 | 358 | 16 | 298 | 1.66e-82 | 253 |
| MsG0680033612.01.T01 | AT4G11660 | 43.407 | 364 | 124 | 9 | 25 | 347 | 51 | 373 | 5.61e-74 | 234 |
| MsG0680033612.01.T01 | AT1G46264 | 43.636 | 220 | 104 | 2 | 28 | 245 | 29 | 230 | 1.28e-52 | 178 |
| MsG0680033612.01.T01 | AT4G36990 | 40.851 | 235 | 81 | 5 | 21 | 244 | 2 | 189 | 7.85e-48 | 163 |
| MsG0680033612.01.T01 | AT2G41690 | 37.991 | 229 | 106 | 3 | 18 | 246 | 25 | 217 | 2.69e-40 | 142 |
| MsG0680033612.01.T01 | AT4G17750 | 54.167 | 120 | 37 | 1 | 23 | 142 | 42 | 143 | 4.44e-36 | 137 |
| MsG0680033612.01.T01 | AT1G32330 | 56.250 | 112 | 31 | 1 | 31 | 142 | 35 | 128 | 2.66e-35 | 135 |
| MsG0680033612.01.T01 | AT1G32330 | 56.250 | 112 | 31 | 1 | 31 | 142 | 35 | 128 | 2.68e-35 | 135 |
| MsG0680033612.01.T01 | AT3G22830 | 51.562 | 128 | 44 | 1 | 15 | 142 | 43 | 152 | 3.87e-35 | 133 |
| MsG0680033612.01.T01 | AT3G02990 | 56.140 | 114 | 31 | 2 | 29 | 142 | 20 | 114 | 3.24e-34 | 131 |
| MsG0680033612.01.T01 | AT5G43840 | 50.420 | 119 | 41 | 1 | 31 | 149 | 17 | 117 | 9.84e-34 | 126 |
| MsG0680033612.01.T01 | AT3G51910 | 46.667 | 120 | 46 | 1 | 31 | 150 | 27 | 128 | 5.03e-33 | 124 |
| MsG0680033612.01.T01 | AT5G16820 | 55.263 | 114 | 32 | 2 | 29 | 142 | 24 | 118 | 1.97e-32 | 127 |
| MsG0680033612.01.T01 | AT5G16820 | 55.263 | 114 | 32 | 2 | 29 | 142 | 24 | 118 | 1.97e-32 | 127 |
| MsG0680033612.01.T01 | AT2G26150 | 50.000 | 112 | 38 | 1 | 31 | 142 | 42 | 135 | 7.48e-32 | 123 |
| MsG0680033612.01.T01 | AT2G26150 | 50.000 | 112 | 38 | 1 | 31 | 142 | 42 | 135 | 7.48e-32 | 123 |
| MsG0680033612.01.T01 | AT5G03720 | 37.313 | 201 | 61 | 3 | 33 | 233 | 55 | 190 | 1.88e-31 | 123 |
| MsG0680033612.01.T01 | AT5G03720 | 37.313 | 201 | 61 | 3 | 33 | 233 | 55 | 190 | 1.88e-31 | 123 |
| MsG0680033612.01.T01 | AT3G63350 | 47.826 | 115 | 42 | 1 | 27 | 141 | 22 | 118 | 1.96e-31 | 120 |
| MsG0680033612.01.T01 | AT4G13980 | 47.541 | 122 | 46 | 1 | 34 | 155 | 24 | 127 | 2.88e-31 | 123 |
| MsG0680033612.01.T01 | AT5G54070 | 47.581 | 124 | 43 | 2 | 30 | 149 | 68 | 173 | 1.10e-30 | 119 |
| MsG0680033612.01.T01 | AT3G24520 | 51.327 | 113 | 37 | 1 | 30 | 142 | 14 | 108 | 4.83e-30 | 117 |
| MsG0680033612.01.T01 | AT1G67970 | 36.264 | 182 | 79 | 4 | 33 | 213 | 19 | 164 | 2.06e-27 | 111 |
| MsG0680033612.01.T01 | AT5G45710 | 46.324 | 136 | 51 | 3 | 29 | 164 | 10 | 123 | 2.07e-24 | 102 |
| MsG0680033612.01.T01 | AT5G45710 | 46.324 | 136 | 51 | 3 | 29 | 164 | 10 | 123 | 2.07e-24 | 102 |
| MsG0680033612.01.T01 | AT4G18880 | 50.427 | 117 | 39 | 2 | 26 | 142 | 9 | 106 | 7.85e-24 | 102 |
| MsG0680033612.01.T01 | AT4G18870 | 29.834 | 181 | 92 | 5 | 34 | 198 | 14 | 175 | 2.93e-18 | 84.3 |
| MsG0680033612.01.T01 | AT4G18870 | 38.182 | 110 | 47 | 3 | 29 | 135 | 139 | 230 | 1.24e-15 | 77.0 |
Find 103 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGATTTGTATTCAGATTT+AGG | 0.200860 | 6:+69477653 | MsG0680033612.01.T01:three_prime_UTR |
| CAATTCCCAGAAGTTTCTTA+TGG | 0.268062 | 6:+69477777 | MsG0680033612.01.T01:three_prime_UTR |
| GACTTCCCATAAGAAACTTC+TGG | 0.288945 | 6:-69477783 | None:intergenic |
| AAAGAAAATAATATAATAAT+AGG | 0.291223 | 6:-69476781 | None:intergenic |
| AGAGAAAACGGTTGTGAAAA+TGG | 0.297213 | 6:+69477476 | MsG0680033612.01.T01:CDS |
| TTATTGTGTTTGAAATATTT+AGG | 0.302478 | 6:-69476706 | None:intergenic |
| ATGGATCCACGTTTATTGTT+TGG | 0.338781 | 6:+69476653 | MsG0680033612.01.T01:CDS |
| CATAAGAAACTTCTGGGAAT+TGG | 0.340004 | 6:-69477776 | None:intergenic |
| ATCAAATCAAGCGGCGGATT+CGG | 0.347225 | 6:-69477374 | None:intergenic |
| GAAATGAATCCGAAGCTATT+CGG | 0.407926 | 6:+69477428 | MsG0680033612.01.T01:CDS |
| GTTATTTCGTGGAACGAAGA+TGG | 0.410793 | 6:+69476634 | MsG0680033612.01.T01:CDS |
| AATTCCCAGAAGTTTCTTAT+GGG | 0.416864 | 6:+69477778 | MsG0680033612.01.T01:three_prime_UTR |
| TTCAGATTTAGGTTAGATAG+AGG | 0.420374 | 6:+69477664 | MsG0680033612.01.T01:three_prime_UTR |
| AAAATGAAAATACAGAGAAA+AGG | 0.436916 | 6:-69477835 | None:intergenic |
| GTCAAACTCCGGTGAAGAAC+AGG | 0.439484 | 6:+69477133 | MsG0680033612.01.T01:CDS |
| AAGCTATTCGGTGTGGCGAT+TGG | 0.446939 | 6:+69477440 | MsG0680033612.01.T01:CDS |
| CATGGCTCCTTCACCAGCTC+CGG | 0.458186 | 6:+69476486 | None:intergenic |
| ATAGAGGTGATGAGGGTGTA+GGG | 0.459580 | 6:+69477680 | MsG0680033612.01.T01:three_prime_UTR |
| TTTGAAAATTCCCACCGGTC+AGG | 0.467906 | 6:-69476915 | None:intergenic |
| TCCTTCACCGGTACTGTTGA+CGG | 0.472445 | 6:+69477079 | MsG0680033612.01.T01:CDS |
| TGTTCTTCACCGGAGTTTGA+CGG | 0.477010 | 6:-69477131 | None:intergenic |
| CGGATTCACCGCCGTCATAC+CGG | 0.477029 | 6:-69477354 | None:intergenic |
| ACTTCCCATAAGAAACTTCT+GGG | 0.481494 | 6:-69477782 | None:intergenic |
| GGGTGTTGCAACGATGGTTG+TGG | 0.483511 | 6:-69476530 | None:intergenic |
| GATAGAGGTGATGAGGGTGT+AGG | 0.483545 | 6:+69477679 | MsG0680033612.01.T01:three_prime_UTR |
| AAATGAAAATACAGAGAAAA+GGG | 0.485258 | 6:-69477834 | None:intergenic |
| TAGGTTAGATAGAGGTGATG+AGG | 0.488681 | 6:+69477672 | MsG0680033612.01.T01:three_prime_UTR |
| AGCTCCGCTGGTGCTCGTGC+CGG | 0.493095 | 6:-69477176 | None:intergenic |
| GGTAATACCAACGGCGATGC+CGG | 0.493700 | 6:+69477100 | MsG0680033612.01.T01:CDS |
| AGGTTAGATAGAGGTGATGA+GGG | 0.499681 | 6:+69477673 | MsG0680033612.01.T01:three_prime_UTR |
| ACGTGTGTCGTTTAACCGTA+TGG | 0.506316 | 6:+69477751 | MsG0680033612.01.T01:three_prime_UTR |
| AAATTACTAGCAAAGACAGT+AGG | 0.509112 | 6:-69476679 | None:intergenic |
| ATTGAGTTGACGAACAAAGC+TGG | 0.509850 | 6:-69476734 | None:intergenic |
| ATCGCCACACCGAATAGCTT+CGG | 0.514928 | 6:-69477437 | None:intergenic |
| GCTTGATTTGATACCGGCGG+TGG | 0.515731 | 6:+69477385 | MsG0680033612.01.T01:CDS |
| GGCTCCTTCACCAGCTCCGG+TGG | 0.518505 | 6:+69476489 | MsG0680033612.01.T01:CDS |
| GCAAAACGAGTAAGAGAAAA+CGG | 0.519247 | 6:+69477464 | MsG0680033612.01.T01:CDS |
| GCTCAAGGAAGTAGAGAATC+CGG | 0.523566 | 6:+69477335 | MsG0680033612.01.T01:CDS |
| GGTATTACCGTCAACAGTAC+CGG | 0.536641 | 6:-69477086 | None:intergenic |
| TTCTGGGAATTGGTCCCATA+CGG | 0.537481 | 6:-69477766 | None:intergenic |
| GACGGTTGCAGTTCCTTCAC+CGG | 0.537577 | 6:+69477067 | MsG0680033612.01.T01:CDS |
| CGTGCATCTCCGCGTGCACA+CGG | 0.542038 | 6:-69477712 | None:intergenic |
| TACAACAAAGGTTACATCTA+CGG | 0.546771 | 6:-69477908 | None:intergenic |
| GGCTTCTATGTGAGATTCAA+CGG | 0.547067 | 6:+69476964 | MsG0680033612.01.T01:CDS |
| AAGTAGAGAATCCGGTATGA+CGG | 0.547644 | 6:+69477343 | MsG0680033612.01.T01:CDS |
| GCGGTGGCAAAACGGAGCTC+CGG | 0.549993 | 6:+69477401 | MsG0680033612.01.T01:CDS |
| TGATACCGGCGGTGGCAAAA+CGG | 0.550237 | 6:+69477393 | MsG0680033612.01.T01:CDS |
| CAAACAGACGGTGGTGCTCA+AGG | 0.552332 | 6:+69477320 | MsG0680033612.01.T01:CDS |
| ACAGACACAGACGGCGGTGA+AGG | 0.555353 | 6:-69477008 | None:intergenic |
| GATATCATCGAGTTCTTCAC+CGG | 0.561771 | 6:+69477157 | MsG0680033612.01.T01:CDS |
| CTGTAGAACCAGGTTGATGT+AGG | 0.562831 | 6:-69477526 | None:intergenic |
| TCAAATGAGTATTTCCGAAG+AGG | 0.564250 | 6:+69476933 | MsG0680033612.01.T01:CDS |
| GAATAGATCTCTGAGACTCA+AGG | 0.566281 | 6:-69476555 | None:intergenic |
| TCCGCCGCTTGATTTGATAC+CGG | 0.567676 | 6:+69477379 | MsG0680033612.01.T01:CDS |
| TCGACGAAAACGAGAGATTG+AGG | 0.567733 | 6:+69477201 | MsG0680033612.01.T01:CDS |
| AATAGATCTCTGAGACTCAA+GGG | 0.568049 | 6:-69476554 | None:intergenic |
| CGTGTGTCGTTTAACCGTAT+GGG | 0.569154 | 6:+69477752 | MsG0680033612.01.T01:three_prime_UTR |
| CTTCGGATTCATTTCCTCGC+CGG | 0.573414 | 6:-69477420 | None:intergenic |
| AATTACTAGCAAAGACAGTA+GGG | 0.574678 | 6:-69476678 | None:intergenic |
| CTATCGACGAGGTTATTTCG+TGG | 0.578836 | 6:+69476623 | MsG0680033612.01.T01:CDS |
| GAAATCACCGGCATCGCCGT+TGG | 0.579266 | 6:-69477107 | None:intergenic |
| AGACGTGAAATCTGAACCGT+TGG | 0.579301 | 6:+69477547 | MsG0680033612.01.T01:CDS |
| TGATATCACCTGTTCTTCAC+CGG | 0.582429 | 6:-69477141 | None:intergenic |
| TTCACCGGCACGAGCACCAG+CGG | 0.586382 | 6:+69477172 | MsG0680033612.01.T01:CDS |
| GAGTTTGACGGTGAAATCAC+CGG | 0.589415 | 6:-69477119 | None:intergenic |
| ACAGACGGCGGTGAAGGTGT+TGG | 0.590096 | 6:-69477002 | None:intergenic |
| CTGTTGCGCCTACATCAACC+TGG | 0.590610 | 6:+69477518 | MsG0680033612.01.T01:CDS |
| GATTTCACGTCTGTAGAACC+AGG | 0.591801 | 6:-69477536 | None:intergenic |
| GTGATTTCACCGTCAAACTC+CGG | 0.593053 | 6:+69477122 | MsG0680033612.01.T01:CDS |
| ACCGTCAACAGTACCGGTGA+AGG | 0.597630 | 6:-69477080 | None:intergenic |
| TCACGTTCTTCACAATCCAA+CGG | 0.603923 | 6:-69477563 | None:intergenic |
| TTAGAAAAGTAGTACCTGAC+CGG | 0.612069 | 6:+69476901 | MsG0680033612.01.T01:CDS |
| AGTTTCTACCGCTGTAGTAG+CGG | 0.615493 | 6:-69477036 | None:intergenic |
| AACAATAACAGTCAAACAGA+CGG | 0.625879 | 6:+69477308 | MsG0680033612.01.T01:CDS |
| AAAAGTAGTACCTGACCGGT+GGG | 0.635759 | 6:+69476905 | MsG0680033612.01.T01:CDS |
| GCCGCTTGATTTGATACCGG+CGG | 0.636793 | 6:+69477382 | MsG0680033612.01.T01:CDS |
| GATCTCTGAGACTCAAGGGT+GGG | 0.636992 | 6:-69476550 | None:intergenic |
| TGTTTCTGCCGCTACTACAG+CGG | 0.640818 | 6:+69477028 | MsG0680033612.01.T01:CDS |
| ACTCATTTGAAAATTCCCAC+CGG | 0.642486 | 6:-69476920 | None:intergenic |
| AAGGGTGGGTGTTGCAACGA+TGG | 0.643473 | 6:-69476536 | None:intergenic |
| ACCGCCGGTATCAAATCAAG+CGG | 0.647730 | 6:-69477383 | None:intergenic |
| GCGGCAGAAACAGACACAGA+CGG | 0.648280 | 6:-69477017 | None:intergenic |
| AATCTGAGACCGTGTGCACG+CGG | 0.650854 | 6:+69477703 | MsG0680033612.01.T01:three_prime_UTR |
| GAAAAGTAGTACCTGACCGG+TGG | 0.652133 | 6:+69476904 | MsG0680033612.01.T01:CDS |
| AATTCTAAACAATACAACAA+AGG | 0.657539 | 6:-69477920 | None:intergenic |
| TAGAGAATCCGGTATGACGG+CGG | 0.661957 | 6:+69477346 | MsG0680033612.01.T01:CDS |
| GGCAAAACGGAGCTCCGGCG+AGG | 0.665647 | 6:+69477406 | MsG0680033612.01.T01:CDS |
| CGATAGATTTATCATCGACG+AGG | 0.666770 | 6:-69476606 | None:intergenic |
| GCCGGTATCAAATCAAGCGG+CGG | 0.668235 | 6:-69477380 | None:intergenic |
| ATTTCCGAAGAGGAGAAAAG+CGG | 0.674735 | 6:+69476943 | MsG0680033612.01.T01:CDS |
| CCAGCTCCGGTGGAAAACAA+CGG | 0.682827 | 6:+69476499 | MsG0680033612.01.T01:CDS |
| ACTGTTGACGGTAATACCAA+CGG | 0.686195 | 6:+69477091 | MsG0680033612.01.T01:CDS |
| AGATCTCTGAGACTCAAGGG+TGG | 0.688159 | 6:-69476551 | None:intergenic |
| AGGGTTCCAAACAATAAACG+TGG | 0.693378 | 6:-69476659 | None:intergenic |
| TTTGTGTAAATGACACGTGG+TGG | 0.696706 | 6:+69477634 | MsG0680033612.01.T01:exon |
| GGTAGAAACTACAACAGCGA+CGG | 0.697044 | 6:+69477049 | MsG0680033612.01.T01:CDS |
| CGATGATAAATCTATCGACG+AGG | 0.705066 | 6:+69476612 | MsG0680033612.01.T01:CDS |
| GAATCCGAAGCTATTCGGTG+TGG | 0.707189 | 6:+69477433 | MsG0680033612.01.T01:CDS |
| TTCTATGTGAGATTCAACGG+AGG | 0.744234 | 6:+69476967 | MsG0680033612.01.T01:CDS |
| AATAACAGTCAAACAGACGG+TGG | 0.748040 | 6:+69477311 | MsG0680033612.01.T01:CDS |
| GCAGAAACAGACACAGACGG+CGG | 0.769997 | 6:-69477014 | None:intergenic |
| GTGATAATGATAGAACAGCG+TGG | 0.790835 | 6:+69477582 | MsG0680033612.01.T01:CDS |
| GAGTTTGTGTAAATGACACG+TGG | 0.796727 | 6:+69477631 | MsG0680033612.01.T01:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTATCATAATCTTTTTTT+GGG | - | Chr6:69477994-69478013 | None:intergenic | 15.0% |
| !!! | TTATTGTGTTTGAAATATTT+AGG | - | Chr6:69476709-69476728 | None:intergenic | 15.0% |
| !! | AAAATGAAAATACAGAGAAA+AGG | - | Chr6:69477838-69477857 | None:intergenic | 20.0% |
| !! | AAATGAAAATACAGAGAAAA+GGG | - | Chr6:69477837-69477856 | None:intergenic | 20.0% |
| !! | AATTCTAAACAATACAACAA+AGG | - | Chr6:69477923-69477942 | None:intergenic | 20.0% |
| !!! | GCATTATCATAATCTTTTTT+TGG | - | Chr6:69477995-69478014 | None:intergenic | 20.0% |
| !!! | TTATATGTTTTTGTCAGAAA+CGG | - | Chr6:69476586-69476605 | None:intergenic | 20.0% |
| !!! | GTTTTGTTGATTTTTTTGCA+GGG | + | Chr6:69476876-69476895 | MsG0680033612.01.T01:intron | 25.0% |
| !!! | TGTTTTGTTGATTTTTTTGC+AGG | + | Chr6:69476875-69476894 | MsG0680033612.01.T01:intron | 25.0% |
| !!! | TGTTTTTTTGGGTACTTTTT+GGG | + | Chr6:69477868-69477887 | MsG0680033612.01.T01:three_prime_UTR | 25.0% |
| !!! | TTGTTTTTTTGGGTACTTTT+TGG | + | Chr6:69477867-69477886 | MsG0680033612.01.T01:three_prime_UTR | 25.0% |
| !!! | TTTTGGGATTAAATTAGATG+TGG | + | Chr6:69477884-69477903 | MsG0680033612.01.T01:three_prime_UTR | 25.0% |
| AAATTACTAGCAAAGACAGT+AGG | - | Chr6:69476682-69476701 | None:intergenic | 30.0% | |
| AACAATAACAGTCAAACAGA+CGG | + | Chr6:69477308-69477327 | MsG0680033612.01.T01:CDS | 30.0% | |
| AATTACTAGCAAAGACAGTA+GGG | - | Chr6:69476681-69476700 | None:intergenic | 30.0% | |
| AATTCCCAGAAGTTTCTTAT+GGG | + | Chr6:69477778-69477797 | MsG0680033612.01.T01:three_prime_UTR | 30.0% | |
| GTGGATTTGTATTCAGATTT+AGG | + | Chr6:69477653-69477672 | MsG0680033612.01.T01:three_prime_UTR | 30.0% | |
| TACAACAAAGGTTACATCTA+CGG | - | Chr6:69477911-69477930 | None:intergenic | 30.0% | |
| ! | TTCAGATTTAGGTTAGATAG+AGG | + | Chr6:69477664-69477683 | MsG0680033612.01.T01:three_prime_UTR | 30.0% |
| ACTCATTTGAAAATTCCCAC+CGG | - | Chr6:69476923-69476942 | None:intergenic | 35.0% | |
| ACTTCCCATAAGAAACTTCT+GGG | - | Chr6:69477785-69477804 | None:intergenic | 35.0% | |
| AGAGAAAACGGTTGTGAAAA+TGG | + | Chr6:69477476-69477495 | MsG0680033612.01.T01:CDS | 35.0% | |
| ATGGATCCACGTTTATTGTT+TGG | + | Chr6:69476653-69476672 | MsG0680033612.01.T01:CDS | 35.0% | |
| CAATTCCCAGAAGTTTCTTA+TGG | + | Chr6:69477777-69477796 | MsG0680033612.01.T01:three_prime_UTR | 35.0% | |
| GAAATGAATCCGAAGCTATT+CGG | + | Chr6:69477428-69477447 | MsG0680033612.01.T01:CDS | 35.0% | |
| GCAAAACGAGTAAGAGAAAA+CGG | + | Chr6:69477464-69477483 | MsG0680033612.01.T01:CDS | 35.0% | |
| TCAAATGAGTATTTCCGAAG+AGG | + | Chr6:69476933-69476952 | MsG0680033612.01.T01:CDS | 35.0% | |
| TTAGAAAAGTAGTACCTGAC+CGG | + | Chr6:69476901-69476920 | MsG0680033612.01.T01:CDS | 35.0% | |
| TTGACGAATTGAAGCGATAA+GGG | + | Chr6:69476839-69476858 | MsG0680033612.01.T01:intron | 35.0% | |
| TTTGACGAATTGAAGCGATA+AGG | + | Chr6:69476838-69476857 | MsG0680033612.01.T01:intron | 35.0% | |
| ! | CATAAGAAACTTCTGGGAAT+TGG | - | Chr6:69477779-69477798 | None:intergenic | 35.0% |
| !! | AATAGATCTCTGAGACTCAA+GGG | - | Chr6:69476557-69476576 | None:intergenic | 35.0% |
| AAGTAGAGAATCCGGTATGA+CGG | + | Chr6:69477343-69477362 | MsG0680033612.01.T01:CDS | 40.0% | |
| AATAACAGTCAAACAGACGG+TGG | + | Chr6:69477311-69477330 | MsG0680033612.01.T01:CDS | 40.0% | |
| ACTGTTGACGGTAATACCAA+CGG | + | Chr6:69477091-69477110 | MsG0680033612.01.T01:CDS | 40.0% | |
| AGGGTTCCAAACAATAAACG+TGG | - | Chr6:69476662-69476681 | None:intergenic | 40.0% | |
| AGGTTAGATAGAGGTGATGA+GGG | + | Chr6:69477673-69477692 | MsG0680033612.01.T01:three_prime_UTR | 40.0% | |
| ATTTCCGAAGAGGAGAAAAG+CGG | + | Chr6:69476943-69476962 | MsG0680033612.01.T01:CDS | 40.0% | |
| CGATAGATTTATCATCGACG+AGG | - | Chr6:69476609-69476628 | None:intergenic | 40.0% | |
| CGATGATAAATCTATCGACG+AGG | + | Chr6:69476612-69476631 | MsG0680033612.01.T01:CDS | 40.0% | |
| GACTTCCCATAAGAAACTTC+TGG | - | Chr6:69477786-69477805 | None:intergenic | 40.0% | |
| GAGTTTGTGTAAATGACACG+TGG | + | Chr6:69477631-69477650 | MsG0680033612.01.T01:exon | 40.0% | |
| GATATCATCGAGTTCTTCAC+CGG | + | Chr6:69477157-69477176 | MsG0680033612.01.T01:CDS | 40.0% | |
| GGCTTCTATGTGAGATTCAA+CGG | + | Chr6:69476964-69476983 | MsG0680033612.01.T01:CDS | 40.0% | |
| TAGGTTAGATAGAGGTGATG+AGG | + | Chr6:69477672-69477691 | MsG0680033612.01.T01:three_prime_UTR | 40.0% | |
| TCACGTTCTTCACAATCCAA+CGG | - | Chr6:69477566-69477585 | None:intergenic | 40.0% | |
| TGATATCACCTGTTCTTCAC+CGG | - | Chr6:69477144-69477163 | None:intergenic | 40.0% | |
| TTCTATGTGAGATTCAACGG+AGG | + | Chr6:69476967-69476986 | MsG0680033612.01.T01:CDS | 40.0% | |
| TTTGTGTAAATGACACGTGG+TGG | + | Chr6:69477634-69477653 | MsG0680033612.01.T01:exon | 40.0% | |
| ! | GTGATAATGATAGAACAGCG+TGG | + | Chr6:69477582-69477601 | MsG0680033612.01.T01:CDS | 40.0% |
| ! | GTTATTTCGTGGAACGAAGA+TGG | + | Chr6:69476634-69476653 | MsG0680033612.01.T01:CDS | 40.0% |
| ! | GTTTTTGTCAGAAACGGTGT+AGG | - | Chr6:69476580-69476599 | None:intergenic | 40.0% |
| !! | ATTGAGTTGACGAACAAAGC+TGG | - | Chr6:69476737-69476756 | None:intergenic | 40.0% |
| !! | GAATAGATCTCTGAGACTCA+AGG | - | Chr6:69476558-69476577 | None:intergenic | 40.0% |
| AAAAGTAGTACCTGACCGGT+GGG | + | Chr6:69476905-69476924 | MsG0680033612.01.T01:CDS | 45.0% | |
| AGACGTGAAATCTGAACCGT+TGG | + | Chr6:69477547-69477566 | MsG0680033612.01.T01:CDS | 45.0% | |
| AGTTTCTACCGCTGTAGTAG+CGG | - | Chr6:69477039-69477058 | None:intergenic | 45.0% | |
| GAGTTTGACGGTGAAATCAC+CGG | - | Chr6:69477122-69477141 | None:intergenic | 45.0% | |
| GATTTCACGTCTGTAGAACC+AGG | - | Chr6:69477539-69477558 | None:intergenic | 45.0% | |
| GCTCAAGGAAGTAGAGAATC+CGG | + | Chr6:69477335-69477354 | MsG0680033612.01.T01:CDS | 45.0% | |
| GGTAGAAACTACAACAGCGA+CGG | + | Chr6:69477049-69477068 | MsG0680033612.01.T01:CDS | 45.0% | |
| GGTATTACCGTCAACAGTAC+CGG | - | Chr6:69477089-69477108 | None:intergenic | 45.0% | |
| GTGATTTCACCGTCAAACTC+CGG | + | Chr6:69477122-69477141 | MsG0680033612.01.T01:CDS | 45.0% | |
| TCGACGAAAACGAGAGATTG+AGG | + | Chr6:69477201-69477220 | MsG0680033612.01.T01:CDS | 45.0% | |
| TGTTCTTCACCGGAGTTTGA+CGG | - | Chr6:69477134-69477153 | None:intergenic | 45.0% | |
| TTTGAAAATTCCCACCGGTC+AGG | - | Chr6:69476918-69476937 | None:intergenic | 45.0% | |
| ! | ATAGAGGTGATGAGGGTGTA+GGG | + | Chr6:69477680-69477699 | MsG0680033612.01.T01:three_prime_UTR | 45.0% |
| ! | ATCAAATCAAGCGGCGGATT+CGG | - | Chr6:69477377-69477396 | None:intergenic | 45.0% |
| ! | CTATCGACGAGGTTATTTCG+TGG | + | Chr6:69476623-69476642 | MsG0680033612.01.T01:CDS | 45.0% |
| ! | TTCTGGGAATTGGTCCCATA+CGG | - | Chr6:69477769-69477788 | None:intergenic | 45.0% |
| !! | ACGTGTGTCGTTTAACCGTA+TGG | + | Chr6:69477751-69477770 | MsG0680033612.01.T01:three_prime_UTR | 45.0% |
| !! | CGTGTGTCGTTTAACCGTAT+GGG | + | Chr6:69477752-69477771 | MsG0680033612.01.T01:three_prime_UTR | 45.0% |
| !! | CTGTAGAACCAGGTTGATGT+AGG | - | Chr6:69477529-69477548 | None:intergenic | 45.0% |
| !! | GTTTCTCCGTTGTTTTCCAC+CGG | - | Chr6:69476508-69476527 | None:intergenic | 45.0% |
| !! | AAAGAAAATAATATAATAAT+AGG | - | Chr6:69476784-69476803 | None:intergenic | 5.0% |
| !!! | TATTTTATTTTTGTTTTTTT+GGG | + | Chr6:69477857-69477876 | MsG0680033612.01.T01:three_prime_UTR | 5.0% |
| !!! | TTATTTTATTTTTGTTTTTT+TGG | + | Chr6:69477856-69477875 | MsG0680033612.01.T01:three_prime_UTR | 5.0% |
| ACCGCCGGTATCAAATCAAG+CGG | - | Chr6:69477386-69477405 | None:intergenic | 50.0% | |
| ATCGCCACACCGAATAGCTT+CGG | - | Chr6:69477440-69477459 | None:intergenic | 50.0% | |
| CTTCGGATTCATTTCCTCGC+CGG | - | Chr6:69477423-69477442 | None:intergenic | 50.0% | |
| GAAAAGTAGTACCTGACCGG+TGG | + | Chr6:69476904-69476923 | MsG0680033612.01.T01:CDS | 50.0% | |
| GTCAAACTCCGGTGAAGAAC+AGG | + | Chr6:69477133-69477152 | MsG0680033612.01.T01:CDS | 50.0% | |
| TAGAGAATCCGGTATGACGG+CGG | + | Chr6:69477346-69477365 | MsG0680033612.01.T01:CDS | 50.0% | |
| TCCTTCACCGGTACTGTTGA+CGG | + | Chr6:69477079-69477098 | MsG0680033612.01.T01:CDS | 50.0% | |
| ! | GAATCCGAAGCTATTCGGTG+TGG | + | Chr6:69477433-69477452 | MsG0680033612.01.T01:CDS | 50.0% |
| ! | GATAGAGGTGATGAGGGTGT+AGG | + | Chr6:69477679-69477698 | MsG0680033612.01.T01:three_prime_UTR | 50.0% |
| ! | TCCGCCGCTTGATTTGATAC+CGG | + | Chr6:69477379-69477398 | MsG0680033612.01.T01:CDS | 50.0% |
| ! | TGTTTCTGCCGCTACTACAG+CGG | + | Chr6:69477028-69477047 | MsG0680033612.01.T01:CDS | 50.0% |
| !! | AAGCTATTCGGTGTGGCGAT+TGG | + | Chr6:69477440-69477459 | MsG0680033612.01.T01:CDS | 50.0% |
| !! | AGATCTCTGAGACTCAAGGG+TGG | - | Chr6:69476554-69476573 | None:intergenic | 50.0% |
| !! | GATCTCTGAGACTCAAGGGT+GGG | - | Chr6:69476553-69476572 | None:intergenic | 50.0% |
| !!! | GAAGCCGCTTTTCTCCTCTT+CGG | - | Chr6:69476950-69476969 | None:intergenic | 50.0% |
| CAAACAGACGGTGGTGCTCA+AGG | + | Chr6:69477320-69477339 | MsG0680033612.01.T01:CDS | 55.0% | |
| CCAGCTCCGGTGGAAAACAA+CGG | + | Chr6:69476499-69476518 | MsG0680033612.01.T01:CDS | 55.0% | |
| CTGTTGCGCCTACATCAACC+TGG | + | Chr6:69477518-69477537 | MsG0680033612.01.T01:CDS | 55.0% | |
| GACGGTTGCAGTTCCTTCAC+CGG | + | Chr6:69477067-69477086 | MsG0680033612.01.T01:CDS | 55.0% | |
| GCAGAAACAGACACAGACGG+CGG | - | Chr6:69477017-69477036 | None:intergenic | 55.0% | |
| GCCGGTATCAAATCAAGCGG+CGG | - | Chr6:69477383-69477402 | None:intergenic | 55.0% | |
| GCGGCAGAAACAGACACAGA+CGG | - | Chr6:69477020-69477039 | None:intergenic | 55.0% | |
| GGTAATACCAACGGCGATGC+CGG | + | Chr6:69477100-69477119 | MsG0680033612.01.T01:CDS | 55.0% | |
| TGATACCGGCGGTGGCAAAA+CGG | + | Chr6:69477393-69477412 | MsG0680033612.01.T01:CDS | 55.0% | |
| ! | AAGGGTGGGTGTTGCAACGA+TGG | - | Chr6:69476539-69476558 | None:intergenic | 55.0% |
| ! | AATCTGAGACCGTGTGCACG+CGG | + | Chr6:69477703-69477722 | MsG0680033612.01.T01:three_prime_UTR | 55.0% |
| ! | GCCGCTTGATTTGATACCGG+CGG | + | Chr6:69477382-69477401 | MsG0680033612.01.T01:CDS | 55.0% |
| ! | GCTTGATTTGATACCGGCGG+TGG | + | Chr6:69477385-69477404 | MsG0680033612.01.T01:CDS | 55.0% |
| ! | GGGTGTTGCAACGATGGTTG+TGG | - | Chr6:69476533-69476552 | None:intergenic | 55.0% |
| ! | TTTTCCACCGGAGCTGGTGA+AGG | - | Chr6:69476496-69476515 | None:intergenic | 55.0% |
| ! | TTTTCGTCGAGAAGCTCCGC+TGG | - | Chr6:69477191-69477210 | None:intergenic | 55.0% |
| !! | ACCGTCAACAGTACCGGTGA+AGG | - | Chr6:69477083-69477102 | None:intergenic | 55.0% |
| ACAGACACAGACGGCGGTGA+AGG | - | Chr6:69477011-69477030 | None:intergenic | 60.0% | |
| CGGATTCACCGCCGTCATAC+CGG | - | Chr6:69477357-69477376 | None:intergenic | 60.0% | |
| GAAATCACCGGCATCGCCGT+TGG | - | Chr6:69477110-69477129 | None:intergenic | 60.0% | |
| ! | ACAGACGGCGGTGAAGGTGT+TGG | - | Chr6:69477005-69477024 | None:intergenic | 60.0% |
| !! | CCGTTGTTTTCCACCGGAGC+TGG | - | Chr6:69476502-69476521 | None:intergenic | 60.0% |
| CGTGCATCTCCGCGTGCACA+CGG | - | Chr6:69477715-69477734 | None:intergenic | 65.0% | |
| GCGGTGGCAAAACGGAGCTC+CGG | + | Chr6:69477401-69477420 | MsG0680033612.01.T01:CDS | 65.0% | |
| TTCACCGGCACGAGCACCAG+CGG | + | Chr6:69477172-69477191 | MsG0680033612.01.T01:CDS | 65.0% | |
| ! | GAGCTCCGTTTTGCCACCGC+CGG | - | Chr6:69477401-69477420 | None:intergenic | 65.0% |
| AGCTCCGCTGGTGCTCGTGC+CGG | - | Chr6:69477179-69477198 | None:intergenic | 70.0% | |
| GGCAAAACGGAGCTCCGGCG+AGG | + | Chr6:69477406-69477425 | MsG0680033612.01.T01:CDS | 70.0% | |
| GGCTCCTTCACCAGCTCCGG+TGG | + | Chr6:69476489-69476508 | MsG0680033612.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 69476487 | 69478031 | 69476487 | ID=MsG0680033612.01;Name=MsG0680033612.01 |
| Chr6 | mRNA | 69476487 | 69478031 | 69476487 | ID=MsG0680033612.01.T01;Parent=MsG0680033612.01;Name=MsG0680033612.01.T01;_AED=0.21;_eAED=0.24;_QI=0|0|0.5|1|0|0.5|2|385|359 |
| Chr6 | exon | 69476897 | 69478031 | 69476897 | ID=MsG0680033612.01.T01:exon:2301;Parent=MsG0680033612.01.T01 |
| Chr6 | exon | 69476487 | 69476816 | 69476487 | ID=MsG0680033612.01.T01:exon:2300;Parent=MsG0680033612.01.T01 |
| Chr6 | CDS | 69476487 | 69476816 | 69476487 | ID=MsG0680033612.01.T01:cds;Parent=MsG0680033612.01.T01 |
| Chr6 | CDS | 69476897 | 69477646 | 69476897 | ID=MsG0680033612.01.T01:cds;Parent=MsG0680033612.01.T01 |
| Chr6 | three_prime_UTR | 69477647 | 69478031 | 69477647 | ID=MsG0680033612.01.T01:three_prime_utr;Parent=MsG0680033612.01.T01 |
| Gene Sequence |
| Protein sequence |
