AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033794.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGTTATATTATCTAAT+TGG 0.218269 6:-73669978 None:intergenic
TTGCCATTAGGTTGCTTTCT+TGG 0.248489 6:-73669296 None:intergenic
GGGCAATAGAAAAGGTTAAA+TGG 0.296255 6:+73668478 MsG0680033794.01.T01:three_prime_UTR
TTGCTTGATGTGAGAATATA+AGG 0.310732 6:-73667143 None:intergenic
TCATTGCTTCATTAAGATCT+TGG 0.330442 6:-73668321 None:intergenic
TATAAGTCATCATCAGTTAA+TGG 0.351177 6:-73669509 None:intergenic
GAAATTTCTACTTAAGTTGT+TGG 0.354277 6:+73669871 MsG0680033794.01.T01:three_prime_UTR
TCTTGACTTGTATAAGGCTT+AGG 0.381448 6:+73669562 MsG0680033794.01.T01:three_prime_UTR
GGTGAGCTTGGACGATAGAT+TGG 0.390515 6:-73664849 None:intergenic
GGTTAACATATAGAGCTTAG+AGG 0.399569 6:+73668439 MsG0680033794.01.T01:intron
TATCCACAATCATTGCCATT+AGG 0.411955 6:-73669308 None:intergenic
AGAGGTTCATCAACTTGCTC+AGG 0.419916 6:+73668457 MsG0680033794.01.T01:three_prime_UTR
GAGGCATTTGAATACAATAT+AGG 0.424314 6:-73669904 None:intergenic
GATATTGACGTGGAAATTTG+AGG 0.436889 6:+73669939 MsG0680033794.01.T01:three_prime_UTR
TTGGACGATAGATTGGGAAC+TGG 0.442743 6:-73664842 None:intergenic
GAAGTTGTGGCGCTGATGAT+TGG 0.447810 6:-73664894 None:intergenic
CTGCTAATATTACTAAGTCT+TGG 0.472781 6:+73669363 MsG0680033794.01.T01:three_prime_UTR
ATATGATTTGCGAAACCAAT+GGG 0.486055 6:+73669529 MsG0680033794.01.T01:three_prime_UTR
TAGCCAAGAAAGCAACCTAA+TGG 0.495668 6:+73669293 MsG0680033794.01.T01:intron
TATAAGGCTTAGGATTTACA+TGG 0.496621 6:+73669572 MsG0680033794.01.T01:three_prime_UTR
TGGACGATAGATTGGGAACT+GGG 0.497216 6:-73664841 None:intergenic
CAACCTAATGGCAATGATTG+TGG 0.497368 6:+73669305 MsG0680033794.01.T01:three_prime_UTR
TCATCATCAGTTAATGGCGT+TGG 0.497730 6:-73669503 None:intergenic
CTTGCTCAGGGCAATAGAAA+AGG 0.501448 6:+73668470 MsG0680033794.01.T01:three_prime_UTR
AAGGCTTCATCTTGAGGAGA+AGG 0.502963 6:-73664940 None:intergenic
CAAGAAAACAAGTTGCCCAT+TGG 0.504204 6:-73669544 None:intergenic
TCATCTTGAGGAGAAGGTGT+TGG 0.504643 6:-73664934 None:intergenic
ATTGGGAACTGGGATTCCAT+TGG 0.521282 6:-73664831 None:intergenic
CGTTTCAATCTCAGTTCCAA+TGG 0.524415 6:+73664815 None:intergenic
CTGATACTCCTTTCTCGTAC+AGG 0.525727 6:-73667107 None:intergenic
GTGAGCTTGGACGATAGATT+GGG 0.526226 6:-73664848 None:intergenic
ATTGAGATTGAAACGATAGA+TGG 0.526554 6:-73664870 None:intergenic
GAAACGATAGATGGTGAGCT+TGG 0.541587 6:-73664861 None:intergenic
AGCAAGTATCAACTCAGATA+AGG 0.542459 6:+73667161 MsG0680033794.01.T01:CDS
CAGTTAATAATTATTTGTCA+AGG 0.561328 6:+73668267 MsG0680033794.01.T01:CDS
GAGCACTACCTGTACGAGAA+AGG 0.562293 6:+73667099 MsG0680033794.01.T01:CDS
CAATATCCAAGCTAGAAAAG+AGG 0.563820 6:-73669923 None:intergenic
GAGGTTCATCAACTTGCTCA+GGG 0.572924 6:+73668458 MsG0680033794.01.T01:three_prime_UTR
TATATGATTTGCGAAACCAA+TGG 0.577102 6:+73669528 MsG0680033794.01.T01:three_prime_UTR
TTCTAGCTTGGATATTGACG+TGG 0.599553 6:+73669929 MsG0680033794.01.T01:three_prime_UTR
TAATATTACTAAGTCTTGGA+TGG 0.604101 6:+73669367 MsG0680033794.01.T01:three_prime_UTR
GATCTTGGTGCATTGAACAA+AGG 0.604500 6:-73668306 None:intergenic
TTACGATGATGATTCTGCTG+TGG 0.615064 6:+73669834 MsG0680033794.01.T01:three_prime_UTR
TCTACGTGTGCAGAAGACAC+TGG 0.621651 6:+73668244 MsG0680033794.01.T01:intron
TCTCATCTAGTCGTAAGCAT+CGG 0.642910 6:-73669778 None:intergenic
TATTACTAAGTCTTGGATGG+AGG 0.643493 6:+73669370 MsG0680033794.01.T01:three_prime_UTR
GCTGAAAATTGATTATGATG+TGG 0.703535 6:+73669739 MsG0680033794.01.T01:intron
TGAGTCTGAATCTGAAGTTG+TGG 0.719530 6:-73664907 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATATAATTAATAATA+TGG - Chr6:73665650-73665669 None:intergenic 0.0%
!! ATAATTGATAAAGATAAAAA+AGG - Chr6:73665051-73665070 None:intergenic 10.0%
!! TAATTGATAAAGATAAAAAA+GGG - Chr6:73665050-73665069 None:intergenic 10.0%
!!! AAAATTCAATTTTGATTAAT+GGG + Chr6:73665144-73665163 MsG0680033794.01.T01:intron 10.0%
!!! AAGTTTTTTTTAATTACATA+GGG - Chr6:73667925-73667944 None:intergenic 10.0%
!!! TAAAATTCAATTTTGATTAA+TGG + Chr6:73665143-73665162 MsG0680033794.01.T01:intron 10.0%
!!! TAAGTTTTTTTTAATTACAT+AGG - Chr6:73667926-73667945 None:intergenic 10.0%
!! AAAGAGTTATATTATCTAAT+TGG - Chr6:73669981-73670000 None:intergenic 15.0%
!! GGTATTATACAAAAAATTAT+TGG - Chr6:73669171-73669190 None:intergenic 15.0%
!! TAAACTCAATTATTTAGAAA+GGG + Chr6:73668974-73668993 MsG0680033794.01.T01:intron 15.0%
!! TAATTAATAATATGGAGAAA+TGG - Chr6:73665642-73665661 None:intergenic 15.0%
!! TATACTTACTTATATAGATA+TGG - Chr6:73668168-73668187 None:intergenic 15.0%
!! TATGTAACTAAGTAAATTAA+GGG + Chr6:73665889-73665908 MsG0680033794.01.T01:intron 15.0%
!! TTACATATATAACTTATGTT+TGG + Chr6:73665732-73665751 MsG0680033794.01.T01:intron 15.0%
!! TTATGTAACTAAGTAAATTA+AGG + Chr6:73665888-73665907 MsG0680033794.01.T01:intron 15.0%
!! TTTATAAGTAGTAATATACA+TGG - Chr6:73665842-73665861 None:intergenic 15.0%
!!! AAATTCAATTTTGATTAATG+GGG + Chr6:73665145-73665164 MsG0680033794.01.T01:intron 15.0%
!!! AAATTTGTTTCTTTACTTAT+TGG + Chr6:73667716-73667735 MsG0680033794.01.T01:intron 15.0%
!!! ATCTACAATTCAAAAATTTT+AGG - Chr6:73665208-73665227 None:intergenic 15.0%
!!! CTTTATCAATTATATCTTTT+AGG + Chr6:73665058-73665077 MsG0680033794.01.T01:intron 15.0%
!!! CTTTGATGATTTTTTTAATT+TGG + Chr6:73668418-73668437 MsG0680033794.01.T01:intron 15.0%
!!! GAAAGAGAATTTTTTTTTAT+TGG + Chr6:73667625-73667644 MsG0680033794.01.T01:intron 15.0%
!!! TCTACAATTCAAAAATTTTA+GGG - Chr6:73665207-73665226 None:intergenic 15.0%
!! AACAAAAAAATCTTCATTGA+TGG - Chr6:73665233-73665252 None:intergenic 20.0%
!! AAGATGTATCAATTTAGATT+AGG + Chr6:73667677-73667696 MsG0680033794.01.T01:intron 20.0%
!! AATACTAAAACTAAAGTTCA+GGG + Chr6:73668093-73668112 MsG0680033794.01.T01:intron 20.0%
!! ACTACTTATAAAACTATCAA+AGG + Chr6:73665850-73665869 MsG0680033794.01.T01:intron 20.0%
!! ATATATATGAACTATGAGTT+GGG + Chr6:73665793-73665812 MsG0680033794.01.T01:intron 20.0%
!! ATCAGTTAAATACAAAAGTA+GGG - Chr6:73665361-73665380 None:intergenic 20.0%
!! ATCTGATATAATCAAATAGT+TGG + Chr6:73666214-73666233 MsG0680033794.01.T01:intron 20.0%
!! ATTTAACTGATATTTGTACA+TGG + Chr6:73665370-73665389 MsG0680033794.01.T01:intron 20.0%
!! CAGTTAATAATTATTTGTCA+AGG + Chr6:73668267-73668286 MsG0680033794.01.T01:CDS 20.0%
!! CTAAACTCAATTATTTAGAA+AGG + Chr6:73668973-73668992 MsG0680033794.01.T01:intron 20.0%
!! GAACTAATCACTTTATAAAA+TGG + Chr6:73669259-73669278 MsG0680033794.01.T01:intron 20.0%
!! TAAAGTAAAAAAATTCACTG+AGG + Chr6:73669005-73669024 MsG0680033794.01.T01:intron 20.0%
!! TAAGCCATAATAATGATAAT+TGG - Chr6:73666986-73667005 None:intergenic 20.0%
!! TAATACAAGAAACAATCATA+CGG - Chr6:73668561-73668580 None:intergenic 20.0%
!! TAATACTAAAACTAAAGTTC+AGG + Chr6:73668092-73668111 MsG0680033794.01.T01:intron 20.0%
!! TATATATATGAACTATGAGT+TGG + Chr6:73665792-73665811 MsG0680033794.01.T01:intron 20.0%
!! TATCAGTTAAATACAAAAGT+AGG - Chr6:73665362-73665381 None:intergenic 20.0%
!! TGATAATTGTATCTGATAAT+TGG + Chr6:73666834-73666853 MsG0680033794.01.T01:intron 20.0%
!! TTTACTTAGTTACATAAACT+AGG - Chr6:73665885-73665904 None:intergenic 20.0%
!!! AGAATTTTTGTAAATTGAGA+AGG - Chr6:73667071-73667090 None:intergenic 20.0%
!!! AGAGAAGATTGTAAATTTTA+TGG - Chr6:73668753-73668772 None:intergenic 20.0%
!!! AGTTTTAGTATTATGATCTT+TGG - Chr6:73668085-73668104 None:intergenic 20.0%
!!! ATTGTTTCTTGTATTAAAAG+TGG + Chr6:73668565-73668584 MsG0680033794.01.T01:intron 20.0%
!!! TATTTTGCATATTTTCAGTT+TGG + Chr6:73667547-73667566 MsG0680033794.01.T01:intron 20.0%
!!! TTAGACTGTGAATTTTTTTA+TGG + Chr6:73665252-73665271 MsG0680033794.01.T01:intron 20.0%
! AAAAACTTTCAGCTTGAAAA+AGG - Chr6:73666690-73666709 None:intergenic 25.0%
! AAATATACAGGGAACAATTA+AGG - Chr6:73668537-73668556 None:intergenic 25.0%
! AAGGAAAGTAGTTAAGAAAA+AGG - Chr6:73666671-73666690 None:intergenic 25.0%
! AAGGTTATAGATCAAAGATA+GGG + Chr6:73665553-73665572 MsG0680033794.01.T01:intron 25.0%
! AATCATACGGAAAATATACA+GGG - Chr6:73668548-73668567 None:intergenic 25.0%
! ACGTAGAAGAACAATTATTA+AGG - Chr6:73668231-73668250 None:intergenic 25.0%
! ACTGCCAATTATCATTATTA+TGG + Chr6:73666979-73666998 MsG0680033794.01.T01:intron 25.0%
! AGACATATTCACTCTTAATT+AGG + Chr6:73665442-73665461 MsG0680033794.01.T01:intron 25.0%
! AGTTATACATTTAGTGTTGT+AGG - Chr6:73668400-73668419 None:intergenic 25.0%
! ATAATAATGATAATTGGCAG+TGG - Chr6:73666980-73666999 None:intergenic 25.0%
! ATGAGAATACCTTAACATAA+AGG + Chr6:73666104-73666123 MsG0680033794.01.T01:intron 25.0%
! ATGCAAAATAAACTTTGACA+TGG - Chr6:73667537-73667556 None:intergenic 25.0%
! ATTAAGAGTGAATATGTCTA+TGG - Chr6:73665441-73665460 None:intergenic 25.0%
! CAAAAATGAAATCAAAAGTG+AGG - Chr6:73667025-73667044 None:intergenic 25.0%
! CAAATGTCAAATTATGAGAT+TGG - Chr6:73669610-73669629 None:intergenic 25.0%
! CATCTGACTATTAATATGAT+TGG + Chr6:73665602-73665621 MsG0680033794.01.T01:intron 25.0%
! GAGTGTGTAAATAAACTTAA+CGG - Chr6:73666642-73666661 None:intergenic 25.0%
! GTACAAGATTAAAGAGTAAA+AGG + Chr6:73666796-73666815 MsG0680033794.01.T01:intron 25.0%
! GTAGTGAAACAATATAATCT+TGG + Chr6:73668666-73668685 MsG0680033794.01.T01:intron 25.0%
! TAAAAAAATTCACTGAGGTA+CGG + Chr6:73669010-73669029 MsG0680033794.01.T01:intron 25.0%
! TAAGGTTATAGATCAAAGAT+AGG + Chr6:73665552-73665571 MsG0680033794.01.T01:intron 25.0%
! TAATATAGAAATGTTCGCTA+AGG - Chr6:73666425-73666444 None:intergenic 25.0%
! TAATATTACTAAGTCTTGGA+TGG + Chr6:73669367-73669386 MsG0680033794.01.T01:three_prime_UTR 25.0%
! TACTCTTGTGGTATTATAAT+TGG + Chr6:73665697-73665716 MsG0680033794.01.T01:intron 25.0%
! TATAAGTCATCATCAGTTAA+TGG - Chr6:73669512-73669531 None:intergenic 25.0%
! TATATTAACCTACACATTGT+AGG + Chr6:73669469-73669488 MsG0680033794.01.T01:intron 25.0%
! TGAGAATACCTTAACATAAA+GGG + Chr6:73666105-73666124 MsG0680033794.01.T01:intron 25.0%
! TTATATGCTACAAAAAGCTT+AGG + Chr6:73666525-73666544 MsG0680033794.01.T01:intron 25.0%
! TTGGTTAAGTAGTGTAAAAA+AGG - Chr6:73669152-73669171 None:intergenic 25.0%
!! GAAATTTCTACTTAAGTTGT+TGG + Chr6:73669871-73669890 MsG0680033794.01.T01:three_prime_UTR 25.0%
!! GAAGATTGTAAATTTTATGG+AGG - Chr6:73668750-73668769 None:intergenic 25.0%
!! TTATTACATGAAGTATCCAA+AGG - Chr6:73666351-73666370 None:intergenic 25.0%
!! TTTTTGTAAATTGAGAAGGA+CGG - Chr6:73667067-73667086 None:intergenic 25.0%
!!! AAAAAGGGCTTTTTAGAAAA+AGG - Chr6:73665035-73665054 None:intergenic 25.0%
!!! TGCATTTTAGTTTAGTTGTA+AGG + Chr6:73665534-73665553 MsG0680033794.01.T01:intron 25.0%
!!! TTGATCATCTTGTTATTGAT+TGG + Chr6:73665301-73665320 MsG0680033794.01.T01:intron 25.0%
!!! TTGTTTTCTTGACTTGTATA+AGG + Chr6:73669556-73669575 MsG0680033794.01.T01:three_prime_UTR 25.0%
!!! TTTAGTTTGTCTTCTTTTTC+AGG + Chr6:73667893-73667912 MsG0680033794.01.T01:intron 25.0%
AAAGAAGACAAACTAAACGT+CGG - Chr6:73667890-73667909 None:intergenic 30.0%
AACAAGAACCATACTTACTT+TGG - Chr6:73668511-73668530 None:intergenic 30.0%
AATAATTGAAGTACGTGACT+TGG + Chr6:73666321-73666340 MsG0680033794.01.T01:intron 30.0%
ACAACAGTATCTATTCATGA+AGG - Chr6:73669192-73669211 None:intergenic 30.0%
ACAAGAACCATACTTACTTT+GGG - Chr6:73668510-73668529 None:intergenic 30.0%
AGTAAACGAAACTAAGAGAA+AGG - Chr6:73666291-73666310 None:intergenic 30.0%
ATAAACAATATCAGCAGTCA+AGG - Chr6:73667802-73667821 None:intergenic 30.0%
ATAAACTTAACGGCTAAGAT+TGG - Chr6:73666632-73666651 None:intergenic 30.0%
ATTATTTAGAAAGGGTAGTC+TGG + Chr6:73668982-73669001 MsG0680033794.01.T01:intron 30.0%
ATTGAGATTGAAACGATAGA+TGG - Chr6:73664873-73664892 None:intergenic 30.0%
CAAATGTCAGAATGCTTAAT+TGG - Chr6:73666891-73666910 None:intergenic 30.0%
CAATCATACGGAAAATATAC+AGG - Chr6:73668549-73668568 None:intergenic 30.0%
CTAGCTTAATGATTTAGTAG+TGG - Chr6:73668881-73668900 None:intergenic 30.0%
CTGCTAATATTACTAAGTCT+TGG + Chr6:73669363-73669382 MsG0680033794.01.T01:three_prime_UTR 30.0%
CTTAATAATTATTGCCGATG+AGG + Chr6:73666257-73666276 MsG0680033794.01.T01:intron 30.0%
GAAACAAATTTCTACCACAA+TGG - Chr6:73667707-73667726 None:intergenic 30.0%
GAATACAGTTATTACTCTTG+TGG + Chr6:73665685-73665704 MsG0680033794.01.T01:intron 30.0%
GACAATCAAAAACAAGAGTA+AGG - Chr6:73667758-73667777 None:intergenic 30.0%
GATACAATTATCACAATCAC+CGG - Chr6:73666827-73666846 None:intergenic 30.0%
GCTGAAAATTGATTATGATG+TGG + Chr6:73669739-73669758 MsG0680033794.01.T01:intron 30.0%
GGAGGATAAACAAGAATAAA+AGG - Chr6:73668732-73668751 None:intergenic 30.0%
TAAACTTAACGGCTAAGATT+GGG - Chr6:73666631-73666650 None:intergenic 30.0%
TAGCAATTGGTTGAAACTTA+AGG - Chr6:73666399-73666418 None:intergenic 30.0%
TATAAGGCTTAGGATTTACA+TGG + Chr6:73669572-73669591 MsG0680033794.01.T01:three_prime_UTR 30.0%
TCATTGCTTCATTAAGATCT+TGG - Chr6:73668324-73668343 None:intergenic 30.0%
TTAATTAGGTTATGTGTGCT+CGG + Chr6:73665456-73665475 MsG0680033794.01.T01:intron 30.0%
TTGCTTGATGTGAGAATATA+AGG - Chr6:73667146-73667165 None:intergenic 30.0%
! ATATGATTTGCGAAACCAAT+GGG + Chr6:73669529-73669548 MsG0680033794.01.T01:three_prime_UTR 30.0%
! CCATAAGAGTATGAATAACA+AGG - Chr6:73666166-73666185 None:intergenic 30.0%
! GAGGCATTTGAATACAATAT+AGG - Chr6:73669907-73669926 None:intergenic 30.0%
! GTAGAGAGTAGAATTTTGAA+CGG + Chr6:73669629-73669648 MsG0680033794.01.T01:intron 30.0%
! GTAGTTAAGAAAAAGGTATG+TGG - Chr6:73666664-73666683 None:intergenic 30.0%
! TAAAGAGTAAAAGGTTTTGC+CGG + Chr6:73666805-73666824 MsG0680033794.01.T01:intron 30.0%
! TAGACTTAGCTTGATTACAT+TGG + Chr6:73669037-73669056 MsG0680033794.01.T01:intron 30.0%
! TATATGATTTGCGAAACCAA+TGG + Chr6:73669528-73669547 MsG0680033794.01.T01:three_prime_UTR 30.0%
!! ACAAATAGATCGAAAGTGTT+TGG + Chr6:73668028-73668047 MsG0680033794.01.T01:intron 30.0%
!! CAAATAGATCGAAAGTGTTT+GGG + Chr6:73668029-73668048 MsG0680033794.01.T01:intron 30.0%
!! CCTTGTTATTCATACTCTTA+TGG + Chr6:73666163-73666182 MsG0680033794.01.T01:intron 30.0%
!! GTGTTTTCTCGAATTTGTTT+TGG + Chr6:73665091-73665110 MsG0680033794.01.T01:intron 30.0%
!! TGCTTGCTATGTTAAAATGT+GGG + Chr6:73667474-73667493 MsG0680033794.01.T01:intron 30.0%
!! TTTTTATGGATTGCGTTTGT+TGG + Chr6:73665266-73665285 MsG0680033794.01.T01:intron 30.0%
!!! CAATTTTGATTAATGGGGTT+TGG + Chr6:73665150-73665169 MsG0680033794.01.T01:intron 30.0%
AAAGCAAATTGTTGTCACCA+AGG + Chr6:73668834-73668853 MsG0680033794.01.T01:intron 35.0%
AAAGTAGGGATCAAAAAGAG+AGG - Chr6:73665347-73665366 None:intergenic 35.0%
AAGAAGACAAACTAAACGTC+GGG - Chr6:73667889-73667908 None:intergenic 35.0%
AAGCAAGAAAGTAGACACAT+CGG - Chr6:73664977-73664996 None:intergenic 35.0%
AGATCTAACTCTGTTCTGTA+TGG - Chr6:73669073-73669092 None:intergenic 35.0%
AGCAAGTATCAACTCAGATA+AGG + Chr6:73667161-73667180 MsG0680033794.01.T01:CDS 35.0%
AGCAATACTTGAAACACACA+AGG - Chr6:73667258-73667277 None:intergenic 35.0%
ATAACTGTTCTGTGCTTTCA+CGG + Chr6:73667414-73667433 MsG0680033794.01.T01:intron 35.0%
ATTGAAGACCTACAATGTGT+AGG - Chr6:73669480-73669499 None:intergenic 35.0%
CAACACAACCCTTTATGTTA+AGG - Chr6:73666116-73666135 None:intergenic 35.0%
CAATATCCAAGCTAGAAAAG+AGG - Chr6:73669926-73669945 None:intergenic 35.0%
CCATGTGAATGTACAGTAAA+AGG - Chr6:73668647-73668666 None:intergenic 35.0%
CTTGTATCTGTCTTAATGTG+TGG + Chr6:73669653-73669672 MsG0680033794.01.T01:intron 35.0%
CTTTCTTGGCTACAAATGAA+TGG - Chr6:73669285-73669304 None:intergenic 35.0%
GAAATCAAAAGTGAGGAAGA+AGG - Chr6:73667018-73667037 None:intergenic 35.0%
GAAGAATGTATGTAAGCACA+TGG - Chr6:73666575-73666594 None:intergenic 35.0%
GACTAAAATCCAAGCATACT+GGG - Chr6:73668063-73668082 None:intergenic 35.0%
GATATTGACGTGGAAATTTG+AGG + Chr6:73669939-73669958 MsG0680033794.01.T01:three_prime_UTR 35.0%
GATCTAACTCTGTTCTGTAT+GGG - Chr6:73669072-73669091 None:intergenic 35.0%
GATTGTCACATTGTGAATCT+CGG + Chr6:73667771-73667790 MsG0680033794.01.T01:intron 35.0%
GCAGACTAATAAGTCGTAAA+TGG - Chr6:73666011-73666030 None:intergenic 35.0%
GGGCAATAGAAAAGGTTAAA+TGG + Chr6:73668478-73668497 MsG0680033794.01.T01:three_prime_UTR 35.0%
GGTTAACATATAGAGCTTAG+AGG + Chr6:73668439-73668458 MsG0680033794.01.T01:intron 35.0%
GTAGTAATATACATGGCAGT+AGG - Chr6:73665835-73665854 None:intergenic 35.0%
TAAAAGACACACAAACTGTG+AGG + Chr6:73665919-73665938 MsG0680033794.01.T01:intron 35.0%
TACAGAACAGAGTTAGATCT+AGG + Chr6:73669073-73669092 MsG0680033794.01.T01:intron 35.0%
TATCCACAATCATTGCCATT+AGG - Chr6:73669311-73669330 None:intergenic 35.0%
TATTACTAAGTCTTGGATGG+AGG + Chr6:73669370-73669389 MsG0680033794.01.T01:three_prime_UTR 35.0%
TATTTGTCGAGATACAGAGA+CGG - Chr6:73668015-73668034 None:intergenic 35.0%
TGCTTCTGCTTATAAAAAGC+TGG + Chr6:73669116-73669135 MsG0680033794.01.T01:intron 35.0%
TTCACAGTATATGCACATCA+TGG - Chr6:73666928-73666947 None:intergenic 35.0%
TTCGGTAAGGACATGTAAAT+GGG + Chr6:73665982-73666001 MsG0680033794.01.T01:intron 35.0%
! AAAAGAACCTTTTGCATGGA+GGG - Chr6:73665003-73665022 None:intergenic 35.0%
! AGATCTAGGATTCCATTACA+TGG + Chr6:73669087-73669106 MsG0680033794.01.T01:intron 35.0%
! CCTTTTACTGTACATTCACA+TGG + Chr6:73668644-73668663 MsG0680033794.01.T01:intron 35.0%
! GAAGAAAAGAACCTTTTGCA+TGG - Chr6:73665007-73665026 None:intergenic 35.0%
! GCTTTTTATAAGCAGAAGCA+TGG - Chr6:73669116-73669135 None:intergenic 35.0%
! TAGTCGATTTCCTTGAACAT+GGG - Chr6:73667235-73667254 None:intergenic 35.0%
! TTTGGTTGATGCTGATGTTA+GGG + Chr6:73667854-73667873 MsG0680033794.01.T01:intron 35.0%
!! GTGCTTGCTATGTTAAAATG+TGG + Chr6:73667473-73667492 MsG0680033794.01.T01:intron 35.0%
!! TCTTGACTTGTATAAGGCTT+AGG + Chr6:73669562-73669581 MsG0680033794.01.T01:three_prime_UTR 35.0%
!! TGGAGAAATGGATTAGAACA+AGG - Chr6:73665630-73665649 None:intergenic 35.0%
!! TTTTGGTTGATGCTGATGTT+AGG + Chr6:73667853-73667872 MsG0680033794.01.T01:intron 35.0%
!!! ATCGAAAGTGTTTGGGATTT+TGG + Chr6:73668036-73668055 MsG0680033794.01.T01:intron 35.0%
AAGATTGGGAGTGAAATCAG+TGG - Chr6:73666617-73666636 None:intergenic 40.0%
ACACTTTGTAAGCACAACAG+TGG - Chr6:73667358-73667377 None:intergenic 40.0%
ACCAAAAGAGAGAATTGAGC+TGG - Chr6:73667840-73667859 None:intergenic 40.0%
AGTGAAATCAGTGGTGTGAA+TGG - Chr6:73666608-73666627 None:intergenic 40.0%
ATGTCCTTACCGAACTACTT+AGG - Chr6:73665976-73665995 None:intergenic 40.0%
CAACCTAATGGCAATGATTG+TGG + Chr6:73669305-73669324 MsG0680033794.01.T01:three_prime_UTR 40.0%
CAAGAAAACAAGTTGCCCAT+TGG - Chr6:73669547-73669566 None:intergenic 40.0%
CTCAAGATGAAGCCTTGAAA+AGG + Chr6:73664947-73664966 MsG0680033794.01.T01:intron 40.0%
GCTTGTACTTACGTGTATCA+CGG + Chr6:73665508-73665527 MsG0680033794.01.T01:intron 40.0%
GGACTAAAATCCAAGCATAC+TGG - Chr6:73668064-73668083 None:intergenic 40.0%
GTATCTGTCTTAATGTGTGG+TGG + Chr6:73669656-73669675 MsG0680033794.01.T01:intron 40.0%
GTTCGGTAAGGACATGTAAA+TGG + Chr6:73665981-73666000 MsG0680033794.01.T01:intron 40.0%
GTTTCGTCCCAAAGTAAGTA+TGG + Chr6:73668500-73668519 MsG0680033794.01.T01:intron 40.0%
TAACTGTTCTGTGCTTTCAC+GGG + Chr6:73667415-73667434 MsG0680033794.01.T01:intron 40.0%
TACAAGCAGGAACTCATATC+TGG - Chr6:73665495-73665514 None:intergenic 40.0%
TACATGGCAGTAGGCTATAA+TGG - Chr6:73665826-73665845 None:intergenic 40.0%
TAGCCAAGAAAGCAACCTAA+TGG + Chr6:73669293-73669312 MsG0680033794.01.T01:intron 40.0%
TCATCATCAGTTAATGGCGT+TGG - Chr6:73669506-73669525 None:intergenic 40.0%
TCGGTAAGGACATGTAAATG+GGG + Chr6:73665983-73666002 MsG0680033794.01.T01:intron 40.0%
TCTCACTTCACCATGTATGT+TGG + Chr6:73667201-73667220 MsG0680033794.01.T01:intron 40.0%
TCTCATCTAGTCGTAAGCAT+CGG - Chr6:73669781-73669800 None:intergenic 40.0%
TGATACACGTAAGTACAAGC+AGG - Chr6:73665508-73665527 None:intergenic 40.0%
TGTCCTTACCGAACTACTTA+GGG - Chr6:73665975-73665994 None:intergenic 40.0%
TTACGATGATGATTCTGCTG+TGG + Chr6:73669834-73669853 MsG0680033794.01.T01:three_prime_UTR 40.0%
TTAGATTAGGAGCTCCATTG+TGG + Chr6:73667690-73667709 MsG0680033794.01.T01:intron 40.0%
TTCAGGGACACATACAAACT+AGG + Chr6:73668109-73668128 MsG0680033794.01.T01:intron 40.0%
TTTGTAAGCACAACAGTGGT+GGG - Chr6:73667354-73667373 None:intergenic 40.0%
! AAAGAACCTTTTGCATGGAG+GGG - Chr6:73665002-73665021 None:intergenic 40.0%
! AGGGTATTCCCAAAACACAT+GGG + Chr6:73665946-73665965 MsG0680033794.01.T01:intron 40.0%
! GAAAAGAACCTTTTGCATGG+AGG - Chr6:73665004-73665023 None:intergenic 40.0%
! GACTTATTAGTCTGCCTTAC+CGG + Chr6:73666016-73666035 MsG0680033794.01.T01:intron 40.0%
! GATCTTGGTGCATTGAACAA+AGG - Chr6:73668309-73668328 None:intergenic 40.0%
! GTAGTCGATTTCCTTGAACA+TGG - Chr6:73667236-73667255 None:intergenic 40.0%
! GTCTTAATGTGTGGTGGTTT+TGG + Chr6:73669662-73669681 MsG0680033794.01.T01:intron 40.0%
! TCCAGCTCAATTCTCTCTTT+TGG + Chr6:73667836-73667855 MsG0680033794.01.T01:intron 40.0%
! TGAGTCTGAATCTGAAGTTG+TGG - Chr6:73664910-73664929 None:intergenic 40.0%
! TTCGCTAAGGTACTAGCAAT+TGG - Chr6:73666412-73666431 None:intergenic 40.0%
! TTGCCATTAGGTTGCTTTCT+TGG - Chr6:73669299-73669318 None:intergenic 40.0%
! TTGGTTGATGCTGATGTTAG+GGG + Chr6:73667855-73667874 MsG0680033794.01.T01:intron 40.0%
!! AAGGGTATTCCCAAAACACA+TGG + Chr6:73665945-73665964 MsG0680033794.01.T01:intron 40.0%
!! CTTTTCAAGGCTTCATCTTG+AGG - Chr6:73664949-73664968 None:intergenic 40.0%
!! TTCTAGCTTGGATATTGACG+TGG + Chr6:73669929-73669948 MsG0680033794.01.T01:three_prime_UTR 40.0%
!!! CAAATGCCTCTTTTCTAGCT+TGG + Chr6:73669917-73669936 MsG0680033794.01.T01:three_prime_UTR 40.0%
AAGGCTTCATCTTGAGGAGA+AGG - Chr6:73664943-73664962 None:intergenic 45.0%
ACACAAACTGTGAGGCAGAA+GGG + Chr6:73665927-73665946 MsG0680033794.01.T01:intron 45.0%
ACCTAAGTATACTCACGCTC+GGG + Chr6:73666048-73666067 MsG0680033794.01.T01:intron 45.0%
AGATACGTATCCACCAGTCA+AGG + Chr6:73667588-73667607 MsG0680033794.01.T01:intron 45.0%
AGGTTATGTGTGCTCGGATT+GGG + Chr6:73665462-73665481 MsG0680033794.01.T01:intron 45.0%
AGTGGTGTGAATGGTAGTAG+TGG - Chr6:73666599-73666618 None:intergenic 45.0%
ATACTTAGGTGGTTGCCAAC+CGG - Chr6:73666038-73666057 None:intergenic 45.0%
ATTGGGAACTGGGATTCCAT+TGG - Chr6:73664834-73664853 None:intergenic 45.0%
CACCTAAGTATACTCACGCT+CGG + Chr6:73666047-73666066 MsG0680033794.01.T01:intron 45.0%
CTGATACTCCTTTCTCGTAC+AGG - Chr6:73667110-73667129 None:intergenic 45.0%
CTTGCTCAGGGCAATAGAAA+AGG + Chr6:73668470-73668489 MsG0680033794.01.T01:three_prime_UTR 45.0%
CTTTGTAAGCACAACAGTGG+TGG - Chr6:73667355-73667374 None:intergenic 45.0%
GAAACGATAGATGGTGAGCT+TGG - Chr6:73664864-73664883 None:intergenic 45.0%
GAAGCATGGAGTCCATGTAA+TGG - Chr6:73669102-73669121 None:intergenic 45.0%
GCAAACTGTCACTGAGTATG+TGG - Chr6:73667950-73667969 None:intergenic 45.0%
GCTAAGAAACGAACACACCT+TGG - Chr6:73668854-73668873 None:intergenic 45.0%
GGGTATTCCCAAAACACATG+GGG + Chr6:73665947-73665966 MsG0680033794.01.T01:intron 45.0%
GGTAACTGCTCCAACATACA+TGG - Chr6:73667214-73667233 None:intergenic 45.0%
GTACCCTAAGTAGTTCGGTA+AGG + Chr6:73665969-73665988 MsG0680033794.01.T01:intron 45.0%
GTGAGCTTGGACGATAGATT+GGG - Chr6:73664851-73664870 None:intergenic 45.0%
TAATTATTGCCGATGAGGCG+TGG + Chr6:73666262-73666281 MsG0680033794.01.T01:intron 45.0%
TAGGTTATGTGTGCTCGGAT+TGG + Chr6:73665461-73665480 MsG0680033794.01.T01:intron 45.0%
TGGACGATAGATTGGGAACT+GGG - Chr6:73664844-73664863 None:intergenic 45.0%
TTGGACGATAGATTGGGAAC+TGG - Chr6:73664845-73664864 None:intergenic 45.0%
! AGAGGTTCATCAACTTGCTC+AGG + Chr6:73668457-73668476 MsG0680033794.01.T01:three_prime_UTR 45.0%
! AGTGGTGGGACTCAAAGAAA+TGG - Chr6:73667340-73667359 None:intergenic 45.0%
! ATGGGGTACCCTAAGTAGTT+CGG + Chr6:73665964-73665983 MsG0680033794.01.T01:intron 45.0%
! GAGGTTCATCAACTTGCTCA+GGG + Chr6:73668458-73668477 MsG0680033794.01.T01:three_prime_UTR 45.0%
! GATTTTGGACCCAGTATGCT+TGG + Chr6:73668051-73668070 MsG0680033794.01.T01:intron 45.0%
! TATTAGTCTGCCTTACCGGT+TGG + Chr6:73666020-73666039 MsG0680033794.01.T01:intron 45.0%
! TCATCTTGAGGAGAAGGTGT+TGG - Chr6:73664937-73664956 None:intergenic 45.0%
!! CACATCGGTGTTCCTTTTCA+AGG - Chr6:73664962-73664981 None:intergenic 45.0%
!! TACGTGACTTGGTACTCCTT+TGG + Chr6:73666332-73666351 MsG0680033794.01.T01:intron 45.0%
!! TAGGGTACCCCATGTGTTTT+GGG - Chr6:73665957-73665976 None:intergenic 45.0%
!! TTAGGGTACCCCATGTGTTT+TGG - Chr6:73665958-73665977 None:intergenic 45.0%
!! ATAAGTAATTAATATTAATA+AGG - Chr6:73669452-73669471 None:intergenic 5.0%
!! TTAATTATATTAATTAATTC+TGG + Chr6:73665655-73665674 MsG0680033794.01.T01:intron 5.0%
AAGTCGTGAACCTTGACTGG+TGG - Chr6:73667601-73667620 None:intergenic 50.0%
ACCCGAGCGTGAGTATACTT+AGG - Chr6:73666052-73666071 None:intergenic 50.0%
CACACAAACTGTGAGGCAGA+AGG + Chr6:73665926-73665945 MsG0680033794.01.T01:intron 50.0%
CGAGCGTGAGTATACTTAGG+TGG - Chr6:73666049-73666068 None:intergenic 50.0%
GAGCACTACCTGTACGAGAA+AGG + Chr6:73667099-73667118 MsG0680033794.01.T01:CDS 50.0%
GGAGCAGTTACCCATGTTCA+AGG + Chr6:73667222-73667241 MsG0680033794.01.T01:intron 50.0%
GGTGAGCTTGGACGATAGAT+TGG - Chr6:73664852-73664871 None:intergenic 50.0%
TAAGTATACTCACGCTCGGG+TGG + Chr6:73666051-73666070 MsG0680033794.01.T01:intron 50.0%
TCGAAGTCGTGAACCTTGAC+TGG - Chr6:73667604-73667623 None:intergenic 50.0%
TCTACGTGTGCAGAAGACAC+TGG + Chr6:73668244-73668263 MsG0680033794.01.T01:intron 50.0%
TTGTCGAGATACAGAGACGG+AGG - Chr6:73668012-73668031 None:intergenic 50.0%
!! GAAGTTGTGGCGCTGATGAT+TGG - Chr6:73664897-73664916 None:intergenic 50.0%
GCTTCTCCCCTCCATGCAAA+AGG + Chr6:73664993-73665012 MsG0680033794.01.T01:intron 55.0%
TAGGTGGTTGCCAACCGGTA+AGG - Chr6:73666033-73666052 None:intergenic 55.0%
GAAAGGCTGCCACGCCTCAT+CGG - Chr6:73666274-73666293 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 73664821 73670003 73664821 ID=MsG0680033794.01;Name=MsG0680033794.01
Chr6 mRNA 73664821 73670003 73664821 ID=MsG0680033794.01.T01;Parent=MsG0680033794.01;Name=MsG0680033794.01.T01;_AED=0.50;_eAED=0.50;_QI=13|1|1|1|0|0|7|581|97
Chr6 exon 73669740 73670003 73669740 ID=MsG0680033794.01.T01:exon:3692;Parent=MsG0680033794.01.T01
Chr6 exon 73669491 73669593 73669491 ID=MsG0680033794.01.T01:exon:3691;Parent=MsG0680033794.01.T01
Chr6 exon 73669296 73669391 73669296 ID=MsG0680033794.01.T01:exon:3690;Parent=MsG0680033794.01.T01
Chr6 exon 73668452 73668512 73668452 ID=MsG0680033794.01.T01:exon:3689;Parent=MsG0680033794.01.T01
Chr6 exon 73668257 73668358 73668257 ID=MsG0680033794.01.T01:exon:3688;Parent=MsG0680033794.01.T01
Chr6 exon 73667048 73667182 73667048 ID=MsG0680033794.01.T01:exon:3687;Parent=MsG0680033794.01.T01
Chr6 exon 73664821 73664947 73664821 ID=MsG0680033794.01.T01:exon:3686;Parent=MsG0680033794.01.T01
Chr6 five_prime_UTR 73664821 73664833 73664821 ID=MsG0680033794.01.T01:five_prime_utr;Parent=MsG0680033794.01.T01
Chr6 CDS 73664834 73664947 73664834 ID=MsG0680033794.01.T01:cds;Parent=MsG0680033794.01.T01
Chr6 CDS 73667048 73667182 73667048 ID=MsG0680033794.01.T01:cds;Parent=MsG0680033794.01.T01
Chr6 CDS 73668257 73668301 73668257 ID=MsG0680033794.01.T01:cds;Parent=MsG0680033794.01.T01
Chr6 three_prime_UTR 73668302 73668358 73668302 ID=MsG0680033794.01.T01:three_prime_utr;Parent=MsG0680033794.01.T01
Chr6 three_prime_UTR 73668452 73668512 73668452 ID=MsG0680033794.01.T01:three_prime_utr;Parent=MsG0680033794.01.T01
Chr6 three_prime_UTR 73669296 73669391 73669296 ID=MsG0680033794.01.T01:three_prime_utr;Parent=MsG0680033794.01.T01
Chr6 three_prime_UTR 73669491 73669593 73669491 ID=MsG0680033794.01.T01:three_prime_utr;Parent=MsG0680033794.01.T01
Chr6 three_prime_UTR 73669740 73670003 73669740 ID=MsG0680033794.01.T01:three_prime_utr;Parent=MsG0680033794.01.T01
Gene Sequence

>MsG0680033794.01.T01

ATGGAATCCCAGTTCCCAATCTATCGTCCAAGCTCACCATCTATCGTTTCAATCTCAATTCCAATCATCAGCGCCACAACTTCAGATTCAGACTCAGCAACCAACACCTTCTCCATTTCGTTTAATTTGACCGTCCTTCTCAATTTACAAAAATTCTTCTCGATCGAGCACTACCTGTACGAGAAAGGAGTATCAGTGATCTTCATCGTCCTTATATTCTCACATCAAGCAAGTATCAACTCAGATAAGAAGACACTGGCAGTTAATAATTATTTGTCAAGGACAATAGTTTAG
Protein sequence

>MsG0680033794.01.T01

MESQFPIYRPSSPSIVSISIPIISATTSDSDSATNTFSISFNLTVLLNLQKFFSIEHYLYEKGVSVIFIVLIFSHQASINSDKKTLAVNNYLSRTIV*