Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030138.01.T01 | XP_024632840.1 | 96.667 | 60 | 2 | 0 | 1 | 60 | 37 | 96 | 6.24E-36 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003669.01 | MsG0580030138.01 | 0.811523 | 6.707992e-51 | 4.161749e-48 |
| MsG0580030138.01 | MsG0680033794.01 | 0.811945 | 5.432165e-51 | 3.408025e-48 |
| MsG0280009297.01 | MsG0580030138.01 | 0.836328 | 9.980495e-57 | 1.253784e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030138.01.T01 | MTR_5g077900 | 96.667 | 60 | 2 | 0 | 1 | 60 | 165 | 224 | 2.97e-38 | 126 |
| MsG0580030138.01.T01 | MTR_0057s0200 | 94.915 | 59 | 3 | 0 | 4 | 62 | 1 | 59 | 1.02e-37 | 119 |
| MsG0580030138.01.T01 | MTR_0002s1270 | 96.667 | 60 | 2 | 0 | 1 | 60 | 339 | 398 | 1.75e-37 | 128 |
| MsG0580030138.01.T01 | MTR_4g066320 | 84.127 | 63 | 9 | 1 | 1 | 62 | 59 | 121 | 3.11e-35 | 115 |
| MsG0580030138.01.T01 | MTR_7g046380 | 96.078 | 51 | 2 | 0 | 1 | 51 | 347 | 397 | 1.22e-31 | 112 |
| MsG0580030138.01.T01 | MTR_3g028430 | 74.074 | 54 | 5 | 1 | 9 | 62 | 15 | 59 | 1.62e-21 | 79.0 |
| MsG0580030138.01.T01 | MTR_4g066300 | 90.625 | 32 | 3 | 0 | 2 | 33 | 7 | 38 | 9.83e-16 | 64.3 |
| MsG0580030138.01.T01 | MTR_2g086200 | 61.224 | 49 | 19 | 0 | 13 | 61 | 185 | 233 | 2.29e-15 | 67.4 |
| MsG0580030138.01.T01 | MTR_0462s0020 | 42.105 | 57 | 33 | 0 | 2 | 58 | 235 | 291 | 6.85e-12 | 58.5 |
| MsG0580030138.01.T01 | MTR_2g092960 | 36.066 | 61 | 39 | 0 | 2 | 62 | 1159 | 1219 | 1.25e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCCATTAGGTTGCTTTCT+TGG | 0.248489 | 5:-107171638 | None:intergenic |
| TATAAGTCATCATCAGTTAA+TGG | 0.351177 | 5:-107171852 | None:intergenic |
| TCTTGACTTGTATAAGGCTT+AGG | 0.381448 | 5:+107171905 | MsG0580030138.01.T01:CDS |
| TATCCACAATCATTGCCATT+AGG | 0.411955 | 5:-107171650 | None:intergenic |
| CTGCTAATATTACTAAGTCT+TGG | 0.472781 | 5:+107171705 | MsG0580030138.01.T01:CDS |
| ATATGATTTGCGAAACCAAT+GGG | 0.486055 | 5:+107171872 | MsG0580030138.01.T01:CDS |
| TAGCCAAGAAAGCAACCTAA+TGG | 0.495668 | 5:+107171635 | None:intergenic |
| CAACCTAATGGCAATGATTG+TGG | 0.497368 | 5:+107171647 | MsG0580030138.01.T01:CDS |
| TCATCATCAGTTAATGGCGT+TGG | 0.497730 | 5:-107171846 | None:intergenic |
| CAAGAAAACAAGTTGCCCAT+TGG | 0.504204 | 5:-107171887 | None:intergenic |
| TATATGATTTGCGAAACCAA+TGG | 0.577102 | 5:+107171871 | MsG0580030138.01.T01:CDS |
| TAATATTACTAAGTCTTGGA+TGG | 0.604101 | 5:+107171709 | MsG0580030138.01.T01:CDS |
| TATTACTAAGTCTTGGATGG+AGG | 0.643493 | 5:+107171712 | MsG0580030138.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATATTACTAAGTCTTGGA+TGG | + | Chr5:107171709-107171728 | MsG0580030138.01.T01:CDS | 25.0% |
| ! | TATAAGTCATCATCAGTTAA+TGG | - | Chr5:107171855-107171874 | None:intergenic | 25.0% |
| ! | TATATTAACCTACACATTGT+AGG | + | Chr5:107171812-107171831 | MsG0580030138.01.T01:intron | 25.0% |
| !!! | TTGTTTTCTTGACTTGTATA+AGG | + | Chr5:107171899-107171918 | MsG0580030138.01.T01:CDS | 25.0% |
| CTGCTAATATTACTAAGTCT+TGG | + | Chr5:107171705-107171724 | MsG0580030138.01.T01:CDS | 30.0% | |
| ! | ATATGATTTGCGAAACCAAT+GGG | + | Chr5:107171872-107171891 | MsG0580030138.01.T01:CDS | 30.0% |
| ! | TATATGATTTGCGAAACCAA+TGG | + | Chr5:107171871-107171890 | MsG0580030138.01.T01:CDS | 30.0% |
| ATTGAAGACCTACAATGTGT+AGG | - | Chr5:107171823-107171842 | None:intergenic | 35.0% | |
| TATCCACAATCATTGCCATT+AGG | - | Chr5:107171653-107171672 | None:intergenic | 35.0% | |
| TATTACTAAGTCTTGGATGG+AGG | + | Chr5:107171712-107171731 | MsG0580030138.01.T01:CDS | 35.0% | |
| CAACCTAATGGCAATGATTG+TGG | + | Chr5:107171647-107171666 | MsG0580030138.01.T01:CDS | 40.0% | |
| CAAGAAAACAAGTTGCCCAT+TGG | - | Chr5:107171890-107171909 | None:intergenic | 40.0% | |
| TCATCATCAGTTAATGGCGT+TGG | - | Chr5:107171849-107171868 | None:intergenic | 40.0% | |
| ! | TTGCCATTAGGTTGCTTTCT+TGG | - | Chr5:107171641-107171660 | None:intergenic | 40.0% |
| !! | ATAAGTAATTAATATTAATA+AGG | - | Chr5:107171795-107171814 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 107171638 | 107171926 | 107171638 | ID=MsG0580030138.01;Name=MsG0580030138.01 |
| Chr5 | mRNA | 107171638 | 107171926 | 107171638 | ID=MsG0580030138.01.T01;Parent=MsG0580030138.01;Name=MsG0580030138.01.T01;_AED=0.50;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|62 |
| Chr5 | exon | 107171638 | 107171733 | 107171638 | ID=MsG0580030138.01.T01:exon:1075;Parent=MsG0580030138.01.T01 |
| Chr5 | exon | 107171834 | 107171926 | 107171834 | ID=MsG0580030138.01.T01:exon:1076;Parent=MsG0580030138.01.T01 |
| Chr5 | CDS | 107171638 | 107171733 | 107171638 | ID=MsG0580030138.01.T01:cds;Parent=MsG0580030138.01.T01 |
| Chr5 | CDS | 107171834 | 107171926 | 107171834 | ID=MsG0580030138.01.T01:cds;Parent=MsG0580030138.01.T01 |
| Gene Sequence |
| Protein sequence |
