AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033865.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033865.01.T01 MTR_2g087270 91.969 386 19 1 1 374 1 386 0.0 734
MsG0680033865.01.T01 MTR_4g109500 29.250 400 229 13 1 373 1 373 2.51e-44 162
MsG0680033865.01.T01 MTR_4g109500 29.250 400 229 13 1 373 1 373 2.77e-44 162
MsG0680033865.01.T01 MTR_8g093800 34.034 238 146 3 101 332 152 384 2.13e-39 145
MsG0680033865.01.T01 MTR_4g111985 31.361 338 210 7 50 372 218 548 1.10e-37 143
MsG0680033865.01.T01 MTR_4g111985 34.397 282 165 5 50 317 257 532 2.21e-37 142
MsG0680033865.01.T01 MTR_4g111985 34.286 280 164 5 50 315 218 491 2.31e-37 141
MsG0680033865.01.T01 MTR_3g118535 36.943 157 94 2 142 298 149 300 1.27e-25 108
MsG0680033865.01.T01 MTR_3g077160 34.066 182 110 3 142 323 161 332 5.13e-25 106
MsG0680033865.01.T01 MTR_4g055080 30.418 263 162 8 99 356 71 317 6.61e-25 105
MsG0680033865.01.T01 MTR_4g055080 30.418 263 162 8 99 356 96 342 1.08e-24 104
MsG0680033865.01.T01 MTR_5g004640 36.306 157 95 2 142 298 152 303 4.30e-24 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033865.01.T01 AT1G08460 69.705 373 99 3 14 372 3 375 0.0 546
MsG0680033865.01.T01 AT1G08460 72.993 274 60 3 15 274 4 277 2.62e-147 420
MsG0680033865.01.T01 AT5G61060 28.861 395 229 9 4 369 14 385 2.77e-42 157
MsG0680033865.01.T01 AT5G61070 29.539 369 230 11 12 364 55 409 7.21e-42 155
MsG0680033865.01.T01 AT5G61060 28.861 395 225 10 4 369 14 381 2.64e-41 154
MsG0680033865.01.T01 AT4G33470 28.933 356 195 9 48 362 78 416 2.35e-39 145
MsG0680033865.01.T01 AT3G18520 34.859 284 165 6 48 317 169 446 2.24e-34 133
MsG0680033865.01.T01 AT3G18520 34.752 282 164 5 48 315 169 444 2.36e-34 132
MsG0680033865.01.T01 AT3G18520 34.859 284 165 6 48 317 169 446 3.25e-34 133
MsG0680033865.01.T01 AT3G18520 34.859 284 165 6 48 317 169 446 3.26e-34 133
MsG0680033865.01.T01 AT5G63110 35.165 182 108 3 142 323 152 323 7.40e-26 108
MsG0680033865.01.T01 AT3G44680 30.315 254 158 7 108 356 103 342 7.20e-25 105
MsG0680033865.01.T01 AT4G38130 35.669 157 96 2 142 298 148 299 8.39e-24 102
MsG0680033865.01.T01 AT4G38130 35.669 157 96 2 142 298 148 299 1.18e-23 102
MsG0680033865.01.T01 AT5G35600 33.333 186 102 5 142 323 147 314 2.45e-19 89.4

Find 113 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTTCACTTGTCTGATTT+TGG 0.069131 6:-75177773 None:intergenic
TCATATCAGGCTCAAATTTA+TGG 0.115466 6:-75179774 None:intergenic
CTATCTCTCCTTATGTTTCT+TGG 0.167482 6:+75178105 MsG0680033865.01.T01:CDS
ATGGAAGGCTATAGAGAAAT+TGG 0.218703 6:+75180380 MsG0680033865.01.T01:CDS
CGTCTCGTCCTCCGGATAAT+AGG 0.219824 6:-75180558 None:intergenic
GTACAGTTAGCTTTAGATTC+TGG 0.256578 6:+75179490 MsG0680033865.01.T01:CDS
ATAGATATTGATGTTCATTA+TGG 0.271623 6:+75179532 MsG0680033865.01.T01:CDS
AACCCCAAATCAAAAGTCTT+TGG 0.296253 6:-75177729 None:intergenic
TCAAACATGACATGAAGTTT+TGG 0.343518 6:-75180674 None:intergenic
ATTGATGTTCATTATGGAAA+TGG 0.351697 6:+75179538 MsG0680033865.01.T01:CDS
TATGCTTTGGTTAGGCCTCC+TGG 0.361621 6:+75179406 MsG0680033865.01.T01:CDS
GGCTCAAATTTATGGATTGA+TGG 0.362518 6:-75179766 None:intergenic
TTCCAACACGTCTAAGAAAC+CGG 0.362595 6:-75178024 None:intergenic
GATATGATTGTCTTGGTTGT+AGG 0.362797 6:+75179790 MsG0680033865.01.T01:CDS
CTGCTAGACCGGCGTTGTTA+AGG 0.377714 6:-75179468 None:intergenic
ATACCTTTGCCAAATGGAAC+TGG 0.380604 6:+75179703 MsG0680033865.01.T01:CDS
ACCCCAAATCAAAAGTCTTT+GGG 0.383836 6:-75177728 None:intergenic
TGGAAAAGTTGCTTATGCTT+TGG 0.394414 6:+75179393 MsG0680033865.01.T01:CDS
CCTTATGTTTCTTGGCACCT+TGG 0.399647 6:+75178113 MsG0680033865.01.T01:CDS
TTCTGCTACCGCATCTGTAT+TGG 0.406722 6:-75177910 None:intergenic
CCATTGTCCCCAGTTCCATT+TGG 0.421588 6:-75179712 None:intergenic
TGTTTACATGCAACACTTGA+AGG 0.421754 6:+75180494 MsG0680033865.01.T01:CDS
CTAGGTGATGGAAAAGGTTA+TGG 0.423056 6:+75179667 MsG0680033865.01.T01:CDS
GTGTGAAAAGAAAGGAGCTC+AGG 0.423266 6:-75178152 None:intergenic
GTTTCTCTTCATATGAATCA+TGG 0.423891 6:+75179601 MsG0680033865.01.T01:CDS
ACAGAAAACGTCTCGTCCTC+CGG 0.432609 6:-75180566 None:intergenic
TCACCATGCTCAGCCTTCTC+AGG 0.438971 6:+75179429 MsG0680033865.01.T01:CDS
TTGAACATACCTTTGCCAAA+TGG 0.438998 6:+75179697 MsG0680033865.01.T01:CDS
TTCATATGAATCATGGATCA+TGG 0.439204 6:+75179608 MsG0680033865.01.T01:CDS
TGTTAAGCATTGCCTTCCAT+TGG 0.445060 6:-75180357 None:intergenic
CAACCCTCTCTTCATTCATA+TGG 0.453228 6:+75177884 MsG0680033865.01.T01:exon
ACAACCAAGACAATCATATC+AGG 0.457583 6:-75179787 None:intergenic
TCGATGAAGCACTTGCTTGA+CGG 0.460456 6:+75179367 MsG0680033865.01.T01:CDS
GTTAAGCATTGCCTTCCATT+GGG 0.466444 6:-75180356 None:intergenic
CATTCTTGAACCCTGGATCA+TGG 0.469920 6:+75179308 MsG0680033865.01.T01:CDS
AGATTCTGTTGCCTATTATC+CGG 0.472439 6:+75180547 MsG0680033865.01.T01:CDS
GCTCAGCCTTCTCAGGCTGA+TGG 0.478600 6:+75179436 MsG0680033865.01.T01:CDS
AGCTAACTGTACTGCTAGAC+CGG 0.480094 6:-75179479 None:intergenic
AGTGCAGCATCCCATGATCC+AGG 0.481293 6:-75179319 None:intergenic
CTCAGCCTTCTCAGGCTGAT+GGG 0.482724 6:+75179437 MsG0680033865.01.T01:CDS
ATGCTGCACTTCTTGCCGCC+GGG 0.484367 6:+75179332 MsG0680033865.01.T01:CDS
TAATAGATAAAATGCCTTTG+TGG 0.486516 6:+75180618 MsG0680033865.01.T01:CDS
CAAGAAACATAAGGAGAGAT+AGG 0.488178 6:-75178104 None:intergenic
TGTGGCACATTCTTGAACCC+TGG 0.495198 6:+75179301 MsG0680033865.01.T01:CDS
AAGTTGCTTATGCTTTGGTT+AGG 0.495823 6:+75179398 MsG0680033865.01.T01:CDS
CCCCGGTTTCTTAGACGTGT+TGG 0.500802 6:+75178022 MsG0680033865.01.T01:CDS
GAAGGCTGAGCATGGTGACC+AGG 0.501428 6:-75179424 None:intergenic
TGGAAGGCTATAGAGAAATT+GGG 0.506833 6:+75180381 MsG0680033865.01.T01:CDS
AGAGAGGGTTGGTTGGTCAC+TGG 0.507834 6:-75177872 None:intergenic
AATCAACATCAAAGATTCAA+AGG 0.514781 6:+75177811 MsG0680033865.01.T01:five_prime_UTR
AAGCACTTGCTTGACGGACA+TGG 0.517006 6:+75179373 MsG0680033865.01.T01:CDS
TGGACGACTTCTTATTGTGC+AGG 0.517764 6:+75180439 MsG0680033865.01.T01:CDS
ATTCTTGAACCCTGGATCAT+GGG 0.518742 6:+75179309 MsG0680033865.01.T01:CDS
GATGCTGCACTTCTTGCCGC+CGG 0.519018 6:+75179331 MsG0680033865.01.T01:CDS
TCATATGAATCATGGATCAT+GGG 0.522086 6:+75179609 MsG0680033865.01.T01:CDS
GTGTTTCAGTTTGATCCCAA+TGG 0.522130 6:+75180341 MsG0680033865.01.T01:intron
TACCTTTGCCAAATGGAACT+GGG 0.522843 6:+75179704 MsG0680033865.01.T01:CDS
CTTCTTATTGTGCAGGAAGG+TGG 0.526523 6:+75180446 MsG0680033865.01.T01:CDS
ATGATTCATATGAAGAGAAA+CGG 0.532665 6:-75179599 None:intergenic
CCAAGGTGCCAAGAAACATA+AGG 0.533396 6:-75178113 None:intergenic
TCCAACACGTCTAAGAAACC+GGG 0.536668 6:-75178023 None:intergenic
TGAGCCTGATATGATTGTCT+TGG 0.544113 6:+75179783 MsG0680033865.01.T01:CDS
AAAGGTGAAACTACAACACT+TGG 0.545182 6:+75177829 MsG0680033865.01.T01:five_prime_UTR
TGTATATGCCTTCAATGAGT+TGG 0.547153 6:+75179738 MsG0680033865.01.T01:CDS
TGGAACAACCAACTCATTGA+AGG 0.547472 6:-75179746 None:intergenic
GGAATGCTCAAACACGATAC+CGG 0.549251 6:+75177972 MsG0680033865.01.T01:CDS
TCCCCAGTTCCATTTGGCAA+AGG 0.550741 6:-75179706 None:intergenic
GAACCACTCTGCGGATGAGA+TGG 0.551133 6:-75179634 None:intergenic
CCAAATGGAACTGGGGACAA+TGG 0.552008 6:+75179712 MsG0680033865.01.T01:CDS
GATGAGCTAGGTGATGGAAA+AGG 0.557420 6:+75179661 MsG0680033865.01.T01:CDS
ATGGTCTCAATCCTTAAAAG+AGG 0.557846 6:+75178080 MsG0680033865.01.T01:CDS
AGGCAATGCTTAACAATGGA+AGG 0.558257 6:+75180365 MsG0680033865.01.T01:CDS
CATCGAAGACAGTGTAGTCC+CGG 0.564565 6:-75179350 None:intergenic
GGTTGGTCACTGGTTCAACA+AGG 0.569986 6:-75177862 None:intergenic
TATGGAAATGGAACAGCGGA+GGG 0.571295 6:+75179550 MsG0680033865.01.T01:CDS
TTCAGTTTGATCCCAATGGA+AGG 0.573033 6:+75180345 MsG0680033865.01.T01:intron
CTCAAACACGATACCGGCAA+TGG 0.574453 6:+75177978 MsG0680033865.01.T01:CDS
AGTGGTTCTATTGATGAGCT+AGG 0.577656 6:+75179649 MsG0680033865.01.T01:CDS
AACCAGGAGTGTGAAAAGAA+AGG 0.581611 6:-75178160 None:intergenic
GCAACAGAATCTTGTAGTAG+AGG 0.582073 6:-75180536 None:intergenic
GAATCTTGTAGTAGAGGCAA+TGG 0.582393 6:-75180530 None:intergenic
TCATATGAAGAGAAACGGTA+AGG 0.587254 6:-75179594 None:intergenic
TCAAGAATGTGCCACAACAA+AGG 0.592402 6:-75179294 None:intergenic
CAGTACCCATCAGCCTGAGA+AGG 0.593929 6:-75179442 None:intergenic
CGACTTCTTATTGTGCAGGA+AGG 0.594885 6:+75180443 MsG0680033865.01.T01:CDS
TACTGTTTCCTTAACAACGC+CGG 0.598032 6:+75179460 MsG0680033865.01.T01:CDS
CAGCCTGAGAAGGCTGAGCA+TGG 0.607738 6:-75179432 None:intergenic
TGGAAGGCAATGCTTAACAA+TGG 0.612899 6:+75180361 MsG0680033865.01.T01:CDS
CATATGAATCATGGATCATG+GGG 0.613927 6:+75179610 MsG0680033865.01.T01:CDS
TCTATTGATGAGCTAGGTGA+TGG 0.615901 6:+75179655 MsG0680033865.01.T01:CDS
TGTGAAAAGAAAGGAGCTCA+GGG 0.616383 6:-75178151 None:intergenic
TTCTGTTGCCTATTATCCGG+AGG 0.618398 6:+75180550 MsG0680033865.01.T01:CDS
GGCTGAGCATGGTGACCAGG+AGG 0.619749 6:-75179421 None:intergenic
TGAATGAAGAGAGGGTTGGT+TGG 0.620291 6:-75177879 None:intergenic
AGTTAATCTGTTCTCCACAA+AGG 0.625888 6:-75180632 None:intergenic
ACCTTTGCCAAATGGAACTG+GGG 0.627049 6:+75179705 MsG0680033865.01.T01:CDS
GGATGAGAGCATGTCGACCA+AGG 0.628418 6:-75178130 None:intergenic
ACTGGTTCAACAAGGTGTAG+AGG 0.632452 6:-75177854 None:intergenic
GTGCAGCATCCCATGATCCA+GGG 0.634352 6:-75179318 None:intergenic
TTATGGAAATGGAACAGCGG+AGG 0.635964 6:+75179549 MsG0680033865.01.T01:CDS
CTTGCGACGAGGCACAGTGA+TGG 0.646082 6:+75180419 MsG0680033865.01.T01:CDS
TTTGACACAGGAATTGACCC+CGG 0.658755 6:+75178005 MsG0680033865.01.T01:CDS
TTGCCATATGAATGAAGAGA+GGG 0.680601 6:-75177887 None:intergenic
CATATGAATGAAGAGAGGGT+TGG 0.691264 6:-75177883 None:intergenic
GGGCCATCTCATCCGCAGAG+TGG 0.701407 6:+75179631 MsG0680033865.01.T01:CDS
GTTGCCATATGAATGAAGAG+AGG 0.704424 6:-75177888 None:intergenic
TATGGCAACCAATACAGATG+CGG 0.714104 6:+75177902 MsG0680033865.01.T01:exon
CCAACACGTCTAAGAAACCG+GGG 0.721257 6:-75178022 None:intergenic
TAGTTCATAGTCTTGCGACG+AGG 0.735316 6:+75180408 MsG0680033865.01.T01:CDS
CGAAGACAGTGTAGTCCCGG+CGG 0.735641 6:-75179347 None:intergenic
TCATTATGGAAATGGAACAG+CGG 0.751642 6:+75179546 MsG0680033865.01.T01:CDS
TCATCAATAGAACCACTCTG+CGG 0.759817 6:-75179643 None:intergenic
ATATGAATCATGGATCATGG+GGG 0.775931 6:+75179611 MsG0680033865.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTATTACATAATTTTTAA+TGG - Chr6:75180606-75180625 None:intergenic 10.0%
!! AAATACTGAAATGTTACAAA+TGG - Chr6:75179076-75179095 None:intergenic 20.0%
!! AATACTGAAATGTTACAAAT+GGG - Chr6:75179075-75179094 None:intergenic 20.0%
!! AGTTAGTAGTTATTTAACAT+TGG + Chr6:75178257-75178276 MsG0680033865.01.T01:intron 20.0%
!! ATAGATATTGATGTTCATTA+TGG + Chr6:75179532-75179551 MsG0680033865.01.T01:CDS 20.0%
!! ATCTAATAAATAAACTAACC+AGG - Chr6:75178179-75178198 None:intergenic 20.0%
!! TCTAAAAAGATACACTTTAA+AGG - Chr6:75178466-75178485 None:intergenic 20.0%
!!! AACATTGGAAGTTAAATTTT+GGG + Chr6:75178272-75178291 MsG0680033865.01.T01:intron 20.0%
!!! ATTTAAACATATTTTAGCAG+AGG - Chr6:75180711-75180730 None:intergenic 20.0%
!!! GAACTTTTTAGAATTATCAT+AGG - Chr6:75178992-75179011 None:intergenic 20.0%
!!! TAACATTGGAAGTTAAATTT+TGG + Chr6:75178271-75178290 MsG0680033865.01.T01:intron 20.0%
! AATCAACATCAAAGATTCAA+AGG + Chr6:75177811-75177830 MsG0680033865.01.T01:five_prime_UTR 25.0%
! ACCAACTTATAATGAGTTTA+TGG - Chr6:75178885-75178904 None:intergenic 25.0%
! ACTATTAGAGAAAAGATAAG+AGG - Chr6:75179144-75179163 None:intergenic 25.0%
! ATGATTCATATGAAGAGAAA+CGG - Chr6:75179602-75179621 None:intergenic 25.0%
! ATTGATGTTCATTATGGAAA+TGG + Chr6:75179538-75179557 MsG0680033865.01.T01:CDS 25.0%
! CAATGATTTGAATTCAAGAT+AGG - Chr6:75178374-75178393 None:intergenic 25.0%
! CCAACTTATAATGAGTTTAT+GGG - Chr6:75178884-75178903 None:intergenic 25.0%
! CTATTAGAGAAAAGATAAGA+GGG - Chr6:75179143-75179162 None:intergenic 25.0%
! GAAAAGTAATTCAATGCATA+TGG - Chr6:75178586-75178605 None:intergenic 25.0%
! GAAATAGCACAAAAAAGAAA+AGG - Chr6:75179850-75179869 None:intergenic 25.0%
! TAATAGATAAAATGCCTTTG+TGG + Chr6:75180618-75180637 MsG0680033865.01.T01:CDS 25.0%
! TATGTAAAAACTTCACATGT+TGG - Chr6:75178968-75178987 None:intergenic 25.0%
! TCACTTAAATTGTAAAAGCA+TGG - Chr6:75178855-75178874 None:intergenic 25.0%
!! AAAAGATACACTTTAAAGGT+TGG - Chr6:75178462-75178481 None:intergenic 25.0%
!! CATAATTCAGAATTCAAGTT+CGG + Chr6:75179105-75179124 MsG0680033865.01.T01:intron 25.0%
!! TGTCAAATTGTTTGAAGTAT+AGG - Chr6:75179192-75179211 None:intergenic 25.0%
!!! CTTTGATGTTGATTTTGATT+GGG - Chr6:75177805-75177824 None:intergenic 25.0%
!!! GAGTTTAATTTTAGTGAATG+TGG - Chr6:75180316-75180335 None:intergenic 25.0%
!!! TCTTTGATGTTGATTTTGAT+TGG - Chr6:75177806-75177825 None:intergenic 25.0%
AATAAGCTGAAAACAACTCA+TGG - Chr6:75180046-75180065 None:intergenic 30.0%
ACATAAACGCTTATCAGATA+AGG - Chr6:75180178-75180197 None:intergenic 30.0%
ATTCAATGCATATGGTTATC+AGG - Chr6:75178578-75178597 None:intergenic 30.0%
CATAAACGCTTATCAGATAA+GGG - Chr6:75180177-75180196 None:intergenic 30.0%
CATCACAAATCACAATGAAT+CGG - Chr6:75178509-75178528 None:intergenic 30.0%
CCCATAAACTCATTATAAGT+TGG + Chr6:75178881-75178900 MsG0680033865.01.T01:intron 30.0%
GTTTCTCTTCATATGAATCA+TGG + Chr6:75179601-75179620 MsG0680033865.01.T01:CDS 30.0%
TCATATCAGGCTCAAATTTA+TGG - Chr6:75179777-75179796 None:intergenic 30.0%
TCATATGAATCATGGATCAT+GGG + Chr6:75179609-75179628 MsG0680033865.01.T01:CDS 30.0%
TGGTTTCATTGCATTTGTTT+AGG - Chr6:75180262-75180281 None:intergenic 30.0%
TTCATATGAATCATGGATCA+TGG + Chr6:75179608-75179627 MsG0680033865.01.T01:CDS 30.0%
TTCTTATAGCTAAGATCAGT+TGG + Chr6:75178613-75178632 MsG0680033865.01.T01:intron 30.0%
! ACAGGATAATCAAACACTTT+TGG - Chr6:75180282-75180301 None:intergenic 30.0%
! AGACTTGCATATGACTTTTA+TGG + Chr6:75179916-75179935 MsG0680033865.01.T01:intron 30.0%
! ATTTCACTTGTCTGATTTTG+GGG - Chr6:75177774-75177793 None:intergenic 30.0%
! GACTTGCATATGACTTTTAT+GGG + Chr6:75179917-75179936 MsG0680033865.01.T01:intron 30.0%
! GATTTCACTTGTCTGATTTT+GGG - Chr6:75177775-75177794 None:intergenic 30.0%
! TCAAACATGACATGAAGTTT+TGG - Chr6:75180677-75180696 None:intergenic 30.0%
! TGAACAAAATTGACGTTTTC+TGG + Chr6:75177943-75177962 MsG0680033865.01.T01:CDS 30.0%
! TTGTTTAGGTTTGCATATCA+TGG - Chr6:75180248-75180267 None:intergenic 30.0%
! TTTGTGAAGATTGCATTTTC+TGG + Chr6:75178295-75178314 MsG0680033865.01.T01:intron 30.0%
!! GGTTTTATAAGCCAATTTGT+GGG + Chr6:75178902-75178921 MsG0680033865.01.T01:intron 30.0%
!!! TGGTTTTATAAGCCAATTTG+TGG + Chr6:75178901-75178920 MsG0680033865.01.T01:intron 30.0%
!!! TTGATTTTGATTGGGATTAG+TGG - Chr6:75177797-75177816 None:intergenic 30.0%
AAAGGTGAAACTACAACACT+TGG + Chr6:75177829-75177848 MsG0680033865.01.T01:five_prime_UTR 35.0%
AAGTTGCTTATGCTTTGGTT+AGG + Chr6:75179398-75179417 MsG0680033865.01.T01:CDS 35.0%
ACAACCAAGACAATCATATC+AGG - Chr6:75179790-75179809 None:intergenic 35.0%
ACTGGTAAAAGCTGATAAAG+AGG + Chr6:75179262-75179281 MsG0680033865.01.T01:intron 35.0%
AGATTCTGTTGCCTATTATC+CGG + Chr6:75180547-75180566 MsG0680033865.01.T01:CDS 35.0%
AGTTAATCTGTTCTCCACAA+AGG - Chr6:75180635-75180654 None:intergenic 35.0%
ATATGAATCATGGATCATGG+GGG + Chr6:75179611-75179630 MsG0680033865.01.T01:CDS 35.0%
ATGGAAGGCTATAGAGAAAT+TGG + Chr6:75180380-75180399 MsG0680033865.01.T01:CDS 35.0%
ATGGTCTCAATCCTTAAAAG+AGG + Chr6:75178080-75178099 MsG0680033865.01.T01:CDS 35.0%
ATTGTTCTCATAAGCTATCG+TGG - Chr6:75180081-75180100 None:intergenic 35.0%
CAAGAAACATAAGGAGAGAT+AGG - Chr6:75178107-75178126 None:intergenic 35.0%
CATATGAATCATGGATCATG+GGG + Chr6:75179610-75179629 MsG0680033865.01.T01:CDS 35.0%
CCATAAATTTATCACTTCCG+AGG + Chr6:75178697-75178716 MsG0680033865.01.T01:intron 35.0%
CCTCGGAAGTGATAAATTTA+TGG - Chr6:75178700-75178719 None:intergenic 35.0%
CTATCTCTCCTTATGTTTCT+TGG + Chr6:75178105-75178124 MsG0680033865.01.T01:CDS 35.0%
CTCAGAGTACATAAATGAAC+TGG + Chr6:75179244-75179263 MsG0680033865.01.T01:intron 35.0%
CTGGTAAAAGCTGATAAAGA+GGG + Chr6:75179263-75179282 MsG0680033865.01.T01:intron 35.0%
GATATGATTGTCTTGGTTGT+AGG + Chr6:75179790-75179809 MsG0680033865.01.T01:CDS 35.0%
TATCGTGCTGTAAACAACTT+GGG + Chr6:75178652-75178671 MsG0680033865.01.T01:intron 35.0%
TCATATGAAGAGAAACGGTA+AGG - Chr6:75179597-75179616 None:intergenic 35.0%
TCATTATGGAAATGGAACAG+CGG + Chr6:75179546-75179565 MsG0680033865.01.T01:CDS 35.0%
TGGAAAAGTTGCTTATGCTT+TGG + Chr6:75179393-75179412 MsG0680033865.01.T01:CDS 35.0%
TGGAAGGCTATAGAGAAATT+GGG + Chr6:75180381-75180400 MsG0680033865.01.T01:CDS 35.0%
TGGTAAAAGCTGATAAAGAG+GGG + Chr6:75179264-75179283 MsG0680033865.01.T01:intron 35.0%
TGTATATGCCTTCAATGAGT+TGG + Chr6:75179738-75179757 MsG0680033865.01.T01:CDS 35.0%
TGTTCATAACACCCACAAAT+TGG - Chr6:75178916-75178935 None:intergenic 35.0%
TGTTTACATGCAACACTTGA+AGG + Chr6:75180494-75180513 MsG0680033865.01.T01:CDS 35.0%
TTATCGTGCTGTAAACAACT+TGG + Chr6:75178651-75178670 MsG0680033865.01.T01:intron 35.0%
TTGAACATACCTTTGCCAAA+TGG + Chr6:75179697-75179716 MsG0680033865.01.T01:CDS 35.0%
TTGAACTTCTAATGACTGCA+CGG + Chr6:75178744-75178763 MsG0680033865.01.T01:intron 35.0%
TTGAAGTATAGGCTATAGCA+GGG - Chr6:75179181-75179200 None:intergenic 35.0%
TTGCCATATGAATGAAGAGA+GGG - Chr6:75177890-75177909 None:intergenic 35.0%
! AACGTCAATTTTGTTCACAC+TGG - Chr6:75177940-75177959 None:intergenic 35.0%
! GCACATAATTGAGCACTTTT+CGG + Chr6:75178796-75178815 MsG0680033865.01.T01:intron 35.0%
! GGATTTCACTTGTCTGATTT+TGG - Chr6:75177776-75177795 None:intergenic 35.0%
! GGCTCAAATTTATGGATTGA+TGG - Chr6:75179769-75179788 None:intergenic 35.0%
! TGCATATGACTTTTATGGGA+TGG + Chr6:75179921-75179940 MsG0680033865.01.T01:intron 35.0%
! TTTGAAGTATAGGCTATAGC+AGG - Chr6:75179182-75179201 None:intergenic 35.0%
!! ACACTTTAAAGGTTGGTGAA+TGG - Chr6:75178455-75178474 None:intergenic 35.0%
!! GTACAGTTAGCTTTAGATTC+TGG + Chr6:75179490-75179509 MsG0680033865.01.T01:CDS 35.0%
!!! AATGTGGCTTTGTTTTACAC+AGG - Chr6:75180300-75180319 None:intergenic 35.0%
AAGCTATCGTGGAGAGTTTA+TGG - Chr6:75180070-75180089 None:intergenic 40.0%
AATTGCTCCAGCTGAATTCA+AGG - Chr6:75179026-75179045 None:intergenic 40.0%
AGGCAATGCTTAACAATGGA+AGG + Chr6:75180365-75180384 MsG0680033865.01.T01:CDS 40.0%
ATACCTTTGCCAAATGGAAC+TGG + Chr6:75179703-75179722 MsG0680033865.01.T01:CDS 40.0%
ATTCTTGAACCCTGGATCAT+GGG + Chr6:75179309-75179328 MsG0680033865.01.T01:CDS 40.0%
CAACCCTCTCTTCATTCATA+TGG + Chr6:75177884-75177903 MsG0680033865.01.T01:exon 40.0%
CAGTATTCCTTGAATTCAGC+TGG + Chr6:75179016-75179035 MsG0680033865.01.T01:intron 40.0%
CATATGAATGAAGAGAGGGT+TGG - Chr6:75177886-75177905 None:intergenic 40.0%
GAATCTTGTAGTAGAGGCAA+TGG - Chr6:75180533-75180552 None:intergenic 40.0%
GCAACAGAATCTTGTAGTAG+AGG - Chr6:75180539-75180558 None:intergenic 40.0%
GGTAAAAGCTGATAAAGAGG+GGG + Chr6:75179265-75179284 MsG0680033865.01.T01:intron 40.0%
GTGTTTCAGTTTGATCCCAA+TGG + Chr6:75180341-75180360 MsG0680033865.01.T01:intron 40.0%
GTTGCCATATGAATGAAGAG+AGG - Chr6:75177891-75177910 None:intergenic 40.0%
TACCTTTGCCAAATGGAACT+GGG + Chr6:75179704-75179723 MsG0680033865.01.T01:CDS 40.0%
TACTGTTTCCTTAACAACGC+CGG + Chr6:75179460-75179479 MsG0680033865.01.T01:CDS 40.0%
TATGGCAACCAATACAGATG+CGG + Chr6:75177902-75177921 MsG0680033865.01.T01:exon 40.0%
TCAAGAATGTGCCACAACAA+AGG - Chr6:75179297-75179316 None:intergenic 40.0%
TCATCAATAGAACCACTCTG+CGG - Chr6:75179646-75179665 None:intergenic 40.0%
TCTATTGATGAGCTAGGTGA+TGG + Chr6:75179655-75179674 MsG0680033865.01.T01:CDS 40.0%
TGAGCCTGATATGATTGTCT+TGG + Chr6:75179783-75179802 MsG0680033865.01.T01:CDS 40.0%
TGGAACAACCAACTCATTGA+AGG - Chr6:75179749-75179768 None:intergenic 40.0%
TGGAAGGCAATGCTTAACAA+TGG + Chr6:75180361-75180380 MsG0680033865.01.T01:CDS 40.0%
TGTGAAAAGAAAGGAGCTCA+GGG - Chr6:75178154-75178173 None:intergenic 40.0%
TGTGTCAAAAACTCCATTGC+CGG - Chr6:75177994-75178013 None:intergenic 40.0%
TTCAGTTTGATCCCAATGGA+AGG + Chr6:75180345-75180364 MsG0680033865.01.T01:intron 40.0%
TTCCAACACGTCTAAGAAAC+CGG - Chr6:75178027-75178046 None:intergenic 40.0%
! AACCAGGAGTGTGAAAAGAA+AGG - Chr6:75178163-75178182 None:intergenic 40.0%
! AGTGGTTCTATTGATGAGCT+AGG + Chr6:75179649-75179668 MsG0680033865.01.T01:CDS 40.0%
! GAGAGATAGGACCTCTTTTA+AGG - Chr6:75178094-75178113 None:intergenic 40.0%
! GTTAAGCATTGCCTTCCATT+GGG - Chr6:75180359-75180378 None:intergenic 40.0%
! TGGGTGTTATGAACATGTCA+TGG + Chr6:75178921-75178940 MsG0680033865.01.T01:intron 40.0%
! TGTTAAGCATTGCCTTCCAT+TGG - Chr6:75180360-75180379 None:intergenic 40.0%
!! ATTGACGTTTTCTGGCATGA+AGG + Chr6:75177951-75177970 MsG0680033865.01.T01:CDS 40.0%
!! CTAGGTGATGGAAAAGGTTA+TGG + Chr6:75179667-75179686 MsG0680033865.01.T01:CDS 40.0%
!! GACTTTTATGGGATGGACTT+AGG + Chr6:75179928-75179947 MsG0680033865.01.T01:intron 40.0%
AACTTCTAATGACTGCACGG+TGG + Chr6:75178747-75178766 MsG0680033865.01.T01:intron 45.0%
ACCTTTGCCAAATGGAACTG+GGG + Chr6:75179705-75179724 MsG0680033865.01.T01:CDS 45.0%
ACTTCTAATGACTGCACGGT+GGG + Chr6:75178748-75178767 MsG0680033865.01.T01:intron 45.0%
AGCTAACTGTACTGCTAGAC+CGG - Chr6:75179482-75179501 None:intergenic 45.0%
CATTCTTGAACCCTGGATCA+TGG + Chr6:75179308-75179327 MsG0680033865.01.T01:CDS 45.0%
CCTTATGTTTCTTGGCACCT+TGG + Chr6:75178113-75178132 MsG0680033865.01.T01:CDS 45.0%
CGACTTCTTATTGTGCAGGA+AGG + Chr6:75180443-75180462 MsG0680033865.01.T01:CDS 45.0%
CGCAGACAGAGTCAAAAACA+TGG + Chr6:75178061-75178080 MsG0680033865.01.T01:CDS 45.0%
CTTCTTATTGTGCAGGAAGG+TGG + Chr6:75180446-75180465 MsG0680033865.01.T01:CDS 45.0%
GGAATGCTCAAACACGATAC+CGG + Chr6:75177972-75177991 MsG0680033865.01.T01:CDS 45.0%
GGTGTGTATTACGCTCTGTT+TGG - Chr6:75180227-75180246 None:intergenic 45.0%
GTGTGAAAAGAAAGGAGCTC+AGG - Chr6:75178155-75178174 None:intergenic 45.0%
TATGGAAATGGAACAGCGGA+GGG + Chr6:75179550-75179569 MsG0680033865.01.T01:CDS 45.0%
TCCAACACGTCTAAGAAACC+GGG - Chr6:75178026-75178045 None:intergenic 45.0%
TGGACGACTTCTTATTGTGC+AGG + Chr6:75180439-75180458 MsG0680033865.01.T01:CDS 45.0%
TTATGGAAATGGAACAGCGG+AGG + Chr6:75179549-75179568 MsG0680033865.01.T01:CDS 45.0%
TTCTGCTACCGCATCTGTAT+TGG - Chr6:75177913-75177932 None:intergenic 45.0%
TTCTGTTGCCTATTATCCGG+AGG + Chr6:75180550-75180569 MsG0680033865.01.T01:CDS 45.0%
TTTGACACAGGAATTGACCC+CGG + Chr6:75178005-75178024 MsG0680033865.01.T01:CDS 45.0%
! ACTGGTTCAACAAGGTGTAG+AGG - Chr6:75177857-75177876 None:intergenic 45.0%
! CCAAGGTGCCAAGAAACATA+AGG - Chr6:75178116-75178135 None:intergenic 45.0%
! CTCCTTTCTTTTCACACTCC+TGG + Chr6:75178158-75178177 MsG0680033865.01.T01:CDS 45.0%
! GGATGGACTTAGGACATGTT+TGG + Chr6:75179938-75179957 MsG0680033865.01.T01:intron 45.0%
! GGCAATGGAGTTTTTGACAC+AGG + Chr6:75177993-75178012 MsG0680033865.01.T01:CDS 45.0%
! TAGTTCATAGTCTTGCGACG+AGG + Chr6:75180408-75180427 MsG0680033865.01.T01:CDS 45.0%
! TCGATGAAGCACTTGCTTGA+CGG + Chr6:75179367-75179386 MsG0680033865.01.T01:CDS 45.0%
! TGAATGAAGAGAGGGTTGGT+TGG - Chr6:75177882-75177901 None:intergenic 45.0%
! TTGACTCTGTCTGCGTTTTC+AGG - Chr6:75178056-75178075 None:intergenic 45.0%
!! GATGAGCTAGGTGATGGAAA+AGG + Chr6:75179661-75179680 MsG0680033865.01.T01:CDS 45.0%
ACAGAAAACGTCTCGTCCTC+CGG - Chr6:75180569-75180588 None:intergenic 50.0%
ACTTCCGAGGCACAGTAGAA+GGG + Chr6:75178710-75178729 MsG0680033865.01.T01:intron 50.0%
CATCGAAGACAGTGTAGTCC+CGG - Chr6:75179353-75179372 None:intergenic 50.0%
CCAAATGGAACTGGGGACAA+TGG + Chr6:75179712-75179731 MsG0680033865.01.T01:CDS 50.0%
CCAACACGTCTAAGAAACCG+GGG - Chr6:75178025-75178044 None:intergenic 50.0%
CCATTGTCCCCAGTTCCATT+TGG - Chr6:75179715-75179734 None:intergenic 50.0%
CTCAAACACGATACCGGCAA+TGG + Chr6:75177978-75177997 MsG0680033865.01.T01:CDS 50.0%
CTTCTAATGACTGCACGGTG+GGG + Chr6:75178749-75178768 MsG0680033865.01.T01:intron 50.0%
GGGGGAAAGATCCTTTGTTG+TGG + Chr6:75179283-75179302 MsG0680033865.01.T01:intron 50.0%
TATGCTTTGGTTAGGCCTCC+TGG + Chr6:75179406-75179425 MsG0680033865.01.T01:CDS 50.0%
TCCCCAGTTCCATTTGGCAA+AGG - Chr6:75179709-75179728 None:intergenic 50.0%
TGTGGCACATTCTTGAACCC+TGG + Chr6:75179301-75179320 MsG0680033865.01.T01:CDS 50.0%
TTCTAATGACTGCACGGTGG+GGG + Chr6:75178750-75178769 MsG0680033865.01.T01:intron 50.0%
! AAGCACTTGCTTGACGGACA+TGG + Chr6:75179373-75179392 MsG0680033865.01.T01:CDS 50.0%
!! GGTTGGTCACTGGTTCAACA+AGG - Chr6:75177865-75177884 None:intergenic 50.0%
AGTGCAGCATCCCATGATCC+AGG - Chr6:75179322-75179341 None:intergenic 55.0%
CACTTCCGAGGCACAGTAGA+AGG + Chr6:75178709-75178728 MsG0680033865.01.T01:intron 55.0%
CAGTACCCATCAGCCTGAGA+AGG - Chr6:75179445-75179464 None:intergenic 55.0%
CCCCGGTTTCTTAGACGTGT+TGG + Chr6:75178022-75178041 MsG0680033865.01.T01:CDS 55.0%
CGTCTCGTCCTCCGGATAAT+AGG - Chr6:75180561-75180580 None:intergenic 55.0%
CTCAGCCTTCTCAGGCTGAT+GGG + Chr6:75179437-75179456 MsG0680033865.01.T01:CDS 55.0%
CTGCTAGACCGGCGTTGTTA+AGG - Chr6:75179471-75179490 None:intergenic 55.0%
GAACCACTCTGCGGATGAGA+TGG - Chr6:75179637-75179656 None:intergenic 55.0%
GCATCCCTTCTACTGTGCCT+CGG - Chr6:75178717-75178736 None:intergenic 55.0%
GTGCAGCATCCCATGATCCA+GGG - Chr6:75179321-75179340 None:intergenic 55.0%
TCACCATGCTCAGCCTTCTC+AGG + Chr6:75179429-75179448 MsG0680033865.01.T01:CDS 55.0%
! GGATGAGAGCATGTCGACCA+AGG - Chr6:75178133-75178152 None:intergenic 55.0%
!! AGAGAGGGTTGGTTGGTCAC+TGG - Chr6:75177875-75177894 None:intergenic 55.0%
ATGCTGCACTTCTTGCCGCC+GGG + Chr6:75179332-75179351 MsG0680033865.01.T01:CDS 60.0%
CAGCCTGAGAAGGCTGAGCA+TGG - Chr6:75179435-75179454 None:intergenic 60.0%
CGAAGACAGTGTAGTCCCGG+CGG - Chr6:75179350-75179369 None:intergenic 60.0%
CTTGCGACGAGGCACAGTGA+TGG + Chr6:75180419-75180438 MsG0680033865.01.T01:CDS 60.0%
GAAGGCTGAGCATGGTGACC+AGG - Chr6:75179427-75179446 None:intergenic 60.0%
GATGCTGCACTTCTTGCCGC+CGG + Chr6:75179331-75179350 MsG0680033865.01.T01:CDS 60.0%
GCTCAGCCTTCTCAGGCTGA+TGG + Chr6:75179436-75179455 MsG0680033865.01.T01:CDS 60.0%
GCACGGTGGGGGTTACTCAC+TGG + Chr6:75178761-75178780 MsG0680033865.01.T01:intron 65.0%
GGCTGAGCATGGTGACCAGG+AGG - Chr6:75179424-75179443 None:intergenic 65.0%
GGGCCATCTCATCCGCAGAG+TGG + Chr6:75179631-75179650 MsG0680033865.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 75177731 75180772 75177731 ID=MsG0680033865.01;Name=MsG0680033865.01
Chr6 mRNA 75177731 75180772 75177731 ID=MsG0680033865.01.T01;Parent=MsG0680033865.01;Name=MsG0680033865.01.T01;_AED=0.27;_eAED=0.27;_QI=172|0.5|0.66|1|1|1|3|120|374
Chr6 exon 75180350 75180772 75180350 ID=MsG0680033865.01.T01:exon:17055;Parent=MsG0680033865.01.T01
Chr6 exon 75179284 75179828 75179284 ID=MsG0680033865.01.T01:exon:17054;Parent=MsG0680033865.01.T01
Chr6 exon 75177731 75178179 75177731 ID=MsG0680033865.01.T01:exon:17053;Parent=MsG0680033865.01.T01
Chr6 five_prime_UTR 75177731 75177902 75177731 ID=MsG0680033865.01.T01:five_prime_utr;Parent=MsG0680033865.01.T01
Chr6 CDS 75177903 75178179 75177903 ID=MsG0680033865.01.T01:cds;Parent=MsG0680033865.01.T01
Chr6 CDS 75179284 75179828 75179284 ID=MsG0680033865.01.T01:cds;Parent=MsG0680033865.01.T01
Chr6 CDS 75180350 75180652 75180350 ID=MsG0680033865.01.T01:cds;Parent=MsG0680033865.01.T01
Chr6 three_prime_UTR 75180653 75180772 75180653 ID=MsG0680033865.01.T01:three_prime_utr;Parent=MsG0680033865.01.T01
Gene Sequence

>MsG0680033865.01.T01

ATGGCAACCAATACAGATGCGGTAGCAGAATCTTCCAGTGTGAACAAAATTGACGTTTTCTGGCATGAAGGAATGCTCAAACACGATACCGGCAATGGAGTTTTTGACACAGGAATTGACCCCGGTTTCTTAGACGTGTTGGAAAAACATCCTGAAAACGCAGACAGAGTCAAAAACATGGTCTCAATCCTTAAAAGAGGTCCTATCTCTCCTTATGTTTCTTGGCACCTTGGTCGACATGCTCTCATCCCTGAGCTCCTTTCTTTTCACACTCCTGGGGGAAAGATCCTTTGTTGTGGCACATTCTTGAACCCTGGATCATGGGATGCTGCACTTCTTGCCGCCGGGACTACACTGTCTTCGATGAAGCACTTGCTTGACGGACATGGAAAAGTTGCTTATGCTTTGGTTAGGCCTCCTGGTCACCATGCTCAGCCTTCTCAGGCTGATGGGTACTGTTTCCTTAACAACGCCGGTCTAGCAGTACAGTTAGCTTTAGATTCTGGATGTAAGAAAGTTGCAGTTATAGATATTGATGTTCATTATGGAAATGGAACAGCGGAGGGATTTTATTCATCTGATAAAGTCCTTACCGTTTCTCTTCATATGAATCATGGATCATGGGGGCCATCTCATCCGCAGAGTGGTTCTATTGATGAGCTAGGTGATGGAAAAGGTTATGGTTATAACTTGAACATACCTTTGCCAAATGGAACTGGGGACAATGGATATGTATATGCCTTCAATGAGTTGGTTGTTCCATCAATCCATAAATTTGAGCCTGATATGATTGTCTTGGTTGTAGGACAAGACTCGAGTGCATTTGATCCCAATGGAAGGCAATGCTTAACAATGGAAGGCTATAGAGAAATTGGGCGTATAGTTCATAGTCTTGCGACGAGGCACAGTGATGGACGACTTCTTATTGTGCAGGAAGGTGGATATCATGTAACATATTCAGCATATTGTTTACATGCAACACTTGAAGGTGTTCTCAATCTACCATTGCCTCTACTACAAGATTCTGTTGCCTATTATCCGGAGGACGAGACGTTTTCTGTCAAAGTTATAGACGCCATTAAAAATTATGTAATAGATAAAATGCCTTTGTGGAGAACAGATTAA
Protein sequence

>MsG0680033865.01.T01

MATNTDAVAESSSVNKIDVFWHEGMLKHDTGNGVFDTGIDPGFLDVLEKHPENADRVKNMVSILKRGPISPYVSWHLGRHALIPELLSFHTPGGKILCCGTFLNPGSWDAALLAAGTTLSSMKHLLDGHGKVAYALVRPPGHHAQPSQADGYCFLNNAGLAVQLALDSGCKKVAVIDIDVHYGNGTAEGFYSSDKVLTVSLHMNHGSWGPSHPQSGSIDELGDGKGYGYNLNIPLPNGTGDNGYVYAFNELVVPSIHKFEPDMIVLVVGQDSSAFDPNGRQCLTMEGYREIGRIVHSLATRHSDGRLLIVQEGGYHVTYSAYCLHATLEGVLNLPLPLLQDSVAYYPEDETFSVKVIDAIKNYVIDKMPLWRTD*