AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033932.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033932.01.T01 MTR_6g089480 85.112 356 12 1 51 406 1 315 0.0 595
MsG0680033932.01.T01 MTR_1g101950 76.257 358 44 1 42 399 21 337 0.0 544
MsG0680033932.01.T01 MTR_6g089480 83.439 314 11 1 51 364 1 273 0.0 514
MsG0680033932.01.T01 MTR_6g089480 84.244 311 8 1 51 361 1 270 0.0 513
MsG0680033932.01.T01 MTR_5g079460 66.852 359 77 2 42 400 65 381 1.52e-158 452
MsG0680033932.01.T01 MTR_1g076850 56.863 357 111 3 44 400 8 321 5.33e-142 407
MsG0680033932.01.T01 MTR_7g075860 57.103 359 111 2 44 400 41 358 3.44e-139 402
MsG0680033932.01.T01 MTR_1g069745 62.090 335 74 2 65 399 4 285 8.18e-138 395
MsG0680033932.01.T01 MTR_1g069490 58.516 364 84 3 36 399 102 398 8.05e-137 397
MsG0680033932.01.T01 MTR_2g098950 55.432 359 117 3 42 400 12 327 1.82e-136 394
MsG0680033932.01.T01 MTR_4g067310 55.801 362 117 3 42 403 13 331 9.79e-136 392
MsG0680033932.01.T01 MTR_1g057810 57.025 363 109 3 36 398 245 560 1.11e-125 374
MsG0680033932.01.T01 MTR_1g105150 52.507 379 119 4 20 398 229 546 3.66e-113 342
MsG0680033932.01.T01 MTR_1g054730 64.557 158 55 1 86 243 146 302 1.09e-61 204
MsG0680033932.01.T01 MTR_1g054730 72.973 74 20 0 326 399 314 387 6.68e-28 114
MsG0680033932.01.T01 MTR_5g044990 27.696 408 217 8 32 402 192 558 1.97e-39 149
MsG0680033932.01.T01 MTR_0046s0180 49.315 146 33 1 254 399 5 109 2.76e-38 134
MsG0680033932.01.T01 MTR_4g019165 29.243 383 234 8 44 406 93 458 3.30e-38 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033932.01.T01 AT5G51830 68.681 364 73 1 37 400 16 338 6.23e-179 502
MsG0680033932.01.T01 AT5G51830 68.681 364 73 1 37 400 16 338 6.23e-179 502
MsG0680033932.01.T01 AT1G66430 63.003 373 96 2 33 405 54 384 9.65e-161 457
MsG0680033932.01.T01 AT4G10260 59.375 352 100 3 41 392 3 311 3.00e-145 416
MsG0680033932.01.T01 AT3G59480 56.704 358 112 3 43 400 9 323 1.77e-138 399
MsG0680033932.01.T01 AT2G31390 55.742 357 115 3 44 400 9 322 3.75e-135 390
MsG0680033932.01.T01 AT1G06020 54.596 359 120 2 44 402 10 325 5.16e-134 388
MsG0680033932.01.T01 AT1G06030 54.294 361 122 3 40 400 7 324 1.68e-133 386
MsG0680033932.01.T01 AT3G54090 29.777 403 205 7 44 406 103 467 3.46e-45 162
MsG0680033932.01.T01 AT1G69200 29.755 326 174 5 70 379 246 532 1.32e-39 149
MsG0680033932.01.T01 AT1G50390 38.208 212 59 2 189 400 1 140 3.98e-37 132

Find 93 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATGCCAATCTTAAATT+TGG 0.189545 6:+76746216 None:intergenic
CAAATTTAAGATTGGCATTA+TGG 0.194373 6:-76746215 MsG0680033932.01.T01:CDS
AAGTCCAAAGATTATAATTT+TGG 0.227394 6:+76743751 None:intergenic
CCTTCCAAAATTATAATCTT+TGG 0.231689 6:-76743755 MsG0680033932.01.T01:three_prime_UTR
GGAATGGCATAATGAGTATA+TGG 0.253977 6:-76746173 MsG0680033932.01.T01:CDS
AATTGTTACTGAAGGGTCTA+AGG 0.261268 6:-76745882 MsG0680033932.01.T01:CDS
TTAAACTAATTGACCCAAAA+TGG 0.292688 6:+76746316 None:intergenic
GTTCACTGGTTGTTTCCTTT+GGG 0.302611 6:-76747152 MsG0680033932.01.T01:CDS
TCTTATATTACAGCTGCTTC+TGG 0.321751 6:+76743859 None:intergenic
AGTTCACTGGTTGTTTCCTT+TGG 0.322009 6:-76747153 MsG0680033932.01.T01:CDS
CCAGTGAACTTGTTTCCTTA+TGG 0.328368 6:+76747166 None:intergenic
TACTAGCGACGCCAACATTT+AGG 0.346554 6:-76747330 MsG0680033932.01.T01:CDS
ACCTCTTTCTGTAACTGTTA+TGG 0.347968 6:+76743927 None:intergenic
TTTAGGATTGAAATCGTATA+CGG 0.351108 6:-76747313 MsG0680033932.01.T01:intron
TGCAAAACAGCTTCCATTGT+AGG 0.361013 6:+76743889 None:intergenic
CAGAACAATGTCGATACTTC+TGG 0.361492 6:-76746584 MsG0680033932.01.T01:CDS
GAGAATGCAACCAGAACTTT+TGG 0.364359 6:+76746248 None:intergenic
ATGAAGATTGCCAAAAGTTC+TGG 0.375368 6:-76746258 MsG0680033932.01.T01:CDS
GGCCGTCTGCCGAGGCTGCT+TGG 0.375839 6:-76746194 MsG0680033932.01.T01:CDS
GCATATCAGCACTTGGATTC+CGG 0.387276 6:+76746480 None:intergenic
TAGGTTGGAGCAGATGAATT+TGG 0.387315 6:-76746632 MsG0680033932.01.T01:CDS
GGATCAAGCCGATGTCATTA+AGG 0.392693 6:-76746151 MsG0680033932.01.T01:intron
CAAGGTATTCATCAATATAG+TGG 0.399518 6:-76743636 None:intergenic
CCAGCACCGGTTGTGTCAAC+AGG 0.401097 6:+76745042 None:intergenic
AAGGTGCCTAAGATGTTGTT+TGG 0.404918 6:-76745660 MsG0680033932.01.T01:CDS
AAAACAACATCATCATCATT+AGG 0.412207 6:+76745938 None:intergenic
ATACGATCCAAATTTAAGAT+TGG 0.426939 6:-76746223 MsG0680033932.01.T01:CDS
TTGAGCTATTAATATAGCTT+AGG 0.432341 6:-76743804 MsG0680033932.01.T01:three_prime_UTR
TGCTGTTGGTATATCTAGAT+TGG 0.434341 6:-76747034 MsG0680033932.01.T01:CDS
GCTGGTGATGCATTTGTTAG+TGG 0.450276 6:-76745024 MsG0680033932.01.T01:CDS
GCTAAGCCAAACAACATCTT+AGG 0.456349 6:+76745654 None:intergenic
AAGATCTGATAACTCATAGT+AGG 0.457227 6:+76745689 None:intergenic
GCTGTTGGTATATCTAGATT+GGG 0.464108 6:-76747033 MsG0680033932.01.T01:CDS
CTGTGCAGGAATTTCGAGGC+AGG 0.465166 6:-76745086 MsG0680033932.01.T01:intron
TCTGCCGAGGCTGCTTGGAA+TGG 0.474211 6:-76746189 MsG0680033932.01.T01:CDS
GTTAAACCTGTTGACACAAC+CGG 0.483410 6:-76745048 MsG0680033932.01.T01:CDS
GAATGGCATAATGAGTATAT+GGG 0.488190 6:-76746172 MsG0680033932.01.T01:CDS
AAACTCTTAATTGTTACTGA+AGG 0.493747 6:-76745890 MsG0680033932.01.T01:CDS
GAATTTCGAGGCAGGGTTGG+AGG 0.497205 6:-76745078 MsG0680033932.01.T01:CDS
TTCCAAGCAGCCTCGGCAGA+CGG 0.504373 6:+76746192 None:intergenic
CCAGGAGATTTCTTGAAAGC+CGG 0.511009 6:+76747075 None:intergenic
AGTGTCGCTCGCCGAGGCAC+CGG 0.511077 6:-76747094 MsG0680033932.01.T01:CDS
TCTTAGAAAAGAAGACCATA+AGG 0.516761 6:-76747181 MsG0680033932.01.T01:CDS
CCGGCTTTCAAGAAATCTCC+TGG 0.521432 6:-76747075 MsG0680033932.01.T01:CDS
TTCACTGGTTGTTTCCTTTG+GGG 0.525662 6:-76747151 MsG0680033932.01.T01:CDS
GCCATAACAGTTACAGAAAG+AGG 0.530881 6:-76743928 MsG0680033932.01.T01:CDS
ACGATTTCAATCCTAAATGT+TGG 0.531769 6:+76747319 None:intergenic
AACTCTTAATTGTTACTGAA+GGG 0.532047 6:-76745889 MsG0680033932.01.T01:CDS
AGCTAATGCAGTCCTTGCAT+TGG 0.532620 6:+76746547 None:intergenic
ATTGTTACTGAAGGGTCTAA+GGG 0.532919 6:-76745881 MsG0680033932.01.T01:CDS
GGCATTATGGCCGTCTGCCG+AGG 0.533060 6:-76746202 MsG0680033932.01.T01:CDS
AACAAATGCATCACCAGCAC+CGG 0.534469 6:+76745029 None:intergenic
CAGGAATTTCGAGGCAGGGT+TGG 0.538929 6:-76745081 MsG0680033932.01.T01:CDS
TGAGAGTAGCAATAAAATCA+AGG 0.539361 6:-76743654 MsG0680033932.01.T01:three_prime_UTR
AGATATACCAACAGCAACAT+TGG 0.540241 6:+76747041 None:intergenic
CCTGTTGACACAACCGGTGC+TGG 0.540265 6:-76745042 MsG0680033932.01.T01:CDS
TGATAGAGATCTCTTAAAGA+AGG 0.540398 6:-76746438 MsG0680033932.01.T01:intron
TTTCTTGAAAGCCGGTGCCT+CGG 0.540957 6:+76747083 None:intergenic
AAGGTATTCATCAATATAGT+GGG 0.544795 6:-76743635 None:intergenic
GACTGACACTTAAGATTGAA+AGG 0.546123 6:+76745711 None:intergenic
TTTCTTTACCTTAATGACAT+CGG 0.550305 6:+76746143 None:intergenic
GCTTCCATTGTAGGAAGTGA+AGG 0.552139 6:+76743898 None:intergenic
CCATAAGGAAACAAGTTCAC+TGG 0.556670 6:-76747166 MsG0680033932.01.T01:CDS
ATTTCTGTGCAGGAATTTCG+AGG 0.557516 6:-76745090 MsG0680033932.01.T01:intron
ATACCAACAGCAACATTGGC+AGG 0.561076 6:+76747045 None:intergenic
GCTCCTGCCAATGTTGCTGT+TGG 0.566054 6:-76747048 MsG0680033932.01.T01:CDS
GTCTATCAACATTTCCCCAA+AGG 0.569388 6:+76747137 None:intergenic
TGAAGAAGCATATCAGCACT+TGG 0.569500 6:+76746473 None:intergenic
CAAGAACTCACGCTCACCGT+CGG 0.572605 6:+76746508 None:intergenic
AGCGACACTCCTCCCACAGT+TGG 0.578152 6:+76747108 None:intergenic
TATGCCATTCCAAGCAGCCT+CGG 0.582212 6:+76746185 None:intergenic
GGGTCAGTGCCGTGTCGTGT+CGG 0.593682 6:-76745751 MsG0680033932.01.T01:CDS
TGTGCAGGAATTTCGAGGCA+GGG 0.593911 6:-76745085 MsG0680033932.01.T01:intron
GTTGGTATATCTAGATTGGG+AGG 0.595607 6:-76747030 MsG0680033932.01.T01:CDS
TTAAGATTGAAAGGTATGAT+CGG 0.600907 6:+76745720 None:intergenic
AATTCCTTCACTTCCTACAA+TGG 0.604354 6:-76743902 MsG0680033932.01.T01:CDS
ACATTGGCAGGAGCTCCACC+AGG 0.606207 6:+76747057 None:intergenic
TGAGGTTTGATGCCAATGCA+AGG 0.607359 6:-76746559 MsG0680033932.01.T01:CDS
TATTAATATAGCTTAGGTGA+TGG 0.616025 6:-76743798 MsG0680033932.01.T01:three_prime_UTR
ATAGACTTTGTGCCAACTGT+GGG 0.616321 6:-76747120 MsG0680033932.01.T01:CDS
AGATGAATTTGGTTACATGT+TGG 0.618988 6:-76746621 MsG0680033932.01.T01:CDS
GACTTTGTGCCAACTGTGGG+AGG 0.621437 6:-76747117 MsG0680033932.01.T01:CDS
GTTATCAGATCTTATAACAA+AGG 0.622421 6:-76745679 MsG0680033932.01.T01:CDS
GATAGACTTTGTGCCAACTG+TGG 0.624305 6:-76747121 MsG0680033932.01.T01:CDS
CATGTATATACAGATAAGTG+AGG 0.626485 6:-76745993 MsG0680033932.01.T01:intron
TTTGTCACACTTAGATCCGA+CGG 0.633560 6:-76746524 MsG0680033932.01.T01:CDS
ATGTCGATACTTCTGGCATG+AGG 0.638705 6:-76746577 MsG0680033932.01.T01:CDS
GGGAGGAGTGTCGCTCGCCG+AGG 0.650456 6:-76747100 MsG0680033932.01.T01:CDS
GCTTTCAAGAAATCTCCTGG+TGG 0.652256 6:-76747072 MsG0680033932.01.T01:CDS
GCAATGTGTGCTGATTTGCA+TGG 0.668568 6:+76746285 None:intergenic
GTGCAGTGTCCGACACGACA+CGG 0.670053 6:+76745742 None:intergenic
GTATATACAGATAAGTGAGG+AGG 0.677503 6:-76745990 MsG0680033932.01.T01:intron
GGGTTGTAGATATTACACAA+AGG 0.751034 6:-76745861 MsG0680033932.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAAAAAATTATTGTAAC+CGG - Chr6:76746252-76746271 MsG0680033932.01.T01:CDS 15.0%
!!! AAAAGAGAATATTATTTTAC+TGG - Chr6:76746420-76746439 MsG0680033932.01.T01:intron 15.0%
!!! GAGATTATTTTTTATACAAA+TGG - Chr6:76746608-76746627 MsG0680033932.01.T01:CDS 15.0%
!!! GTTACAATAATTTTTTACTA+AGG + Chr6:76746252-76746271 None:intergenic 15.0%
!!! TATCTTTGTTTTTTTTTTTG+TGG + Chr6:76746150-76746169 None:intergenic 15.0%
!! AACCTAATACACAAATAATA+AGG + Chr6:76744365-76744384 None:intergenic 20.0%
!! AAGTTTATGTTTATCCAAAT+TGG - Chr6:76745590-76745609 MsG0680033932.01.T01:intron 20.0%
!! ACATCATATACAAATTGATA+TGG + Chr6:76745184-76745203 None:intergenic 20.0%
!! AGTAAAAAATTATTGTAACC+GGG - Chr6:76746253-76746272 MsG0680033932.01.T01:CDS 20.0%
!! ATGCAAATAATTGAATGTAA+TGG - Chr6:76745227-76745246 MsG0680033932.01.T01:intron 20.0%
!! CAAAATCTAAATTAAACCAT+AGG + Chr6:76744643-76744662 None:intergenic 20.0%
!! TAGTAAAAAATTATCGTAAC+CGG - Chr6:76746355-76746374 MsG0680033932.01.T01:intron 20.0%
!! TATTACTAATAACACCAATT+TGG + Chr6:76745607-76745626 None:intergenic 20.0%
!! TTATGGAATTATAGTCTATT+TGG - Chr6:76745629-76745648 MsG0680033932.01.T01:intron 20.0%
!! TTATTATTTGTGTATTAGGT+TGG - Chr6:76744364-76744383 MsG0680033932.01.T01:intron 20.0%
!! TTCCTTATTATTTGTGTATT+AGG - Chr6:76744360-76744379 MsG0680033932.01.T01:intron 20.0%
!! TTTGTTTATTTCATGAAATG+TGG - Chr6:76744610-76744629 MsG0680033932.01.T01:intron 20.0%
!!! AAGTCCAAAGATTATAATTT+TGG + Chr6:76747263-76747282 None:intergenic 20.0%
!!! AATTTTGGAAGGAAAAAAAT+TGG + Chr6:76747248-76747267 None:intergenic 20.0%
!!! ATAATATTTTTACTGGTTTC+TGG - Chr6:76746540-76746559 MsG0680033932.01.T01:CDS 20.0%
!!! ATTAACCTTTTAGTTTTTGA+AGG - Chr6:76747323-76747342 MsG0680033932.01.T01:CDS 20.0%
!!! ATTATATATATTTTCTTGGC+AGG - Chr6:76747013-76747032 MsG0680033932.01.T01:CDS 20.0%
!!! GTGTTATTAGTAATAAGTTA+TGG - Chr6:76745612-76745631 MsG0680033932.01.T01:intron 20.0%
!!! GTTACGATAATTTTTTACTA+AGG + Chr6:76746355-76746374 None:intergenic 20.0%
!!! TAATATTTTTACTGGTTTCT+GGG - Chr6:76746541-76746560 MsG0680033932.01.T01:CDS 20.0%
! AAAACAACATCATCATCATT+AGG + Chr6:76745076-76745095 None:intergenic 25.0%
! AAACAAACCTGAAAAATACT+CGG + Chr6:76746038-76746057 None:intergenic 25.0%
! AAACATGAACAAAATCTTGA+AGG + Chr6:76744111-76744130 None:intergenic 25.0%
! AAACTCTTAATTGTTACTGA+AGG - Chr6:76745121-76745140 MsG0680033932.01.T01:intron 25.0%
! AAATTATACACATGATGTAG+TGG - Chr6:76744888-76744907 MsG0680033932.01.T01:intron 25.0%
! AACTCTTAATTGTTACTGAA+GGG - Chr6:76745122-76745141 MsG0680033932.01.T01:intron 25.0%
! AACTTATTTAAACGCACATA+TGG + Chr6:76745575-76745594 None:intergenic 25.0%
! AAGCAGAAAATTATTGTAAC+CGG + Chr6:76746296-76746315 None:intergenic 25.0%
! ACTTATTTAAACGCACATAT+GGG + Chr6:76745574-76745593 None:intergenic 25.0%
! AGAGATAAAGGAACTAAAAT+TGG - Chr6:76746586-76746605 MsG0680033932.01.T01:CDS 25.0%
! AGTAAAAAATTATCGTAACC+GGG - Chr6:76746356-76746375 MsG0680033932.01.T01:intron 25.0%
! ATCAAATGACAAAAGAAAAG+TGG + Chr6:76744208-76744227 None:intergenic 25.0%
! CATAATGCCAATCTTAAATT+TGG + Chr6:76744798-76744817 None:intergenic 25.0%
! CCTTCCAAAATTATAATCTT+TGG - Chr6:76747256-76747275 MsG0680033932.01.T01:intron 25.0%
! GTAAAAAATTATTGTAACCG+GGG - Chr6:76746254-76746273 MsG0680033932.01.T01:CDS 25.0%
! GTTATCAGATCTTATAACAA+AGG - Chr6:76745332-76745351 MsG0680033932.01.T01:intron 25.0%
! TACAACAAGAACAAAAAATC+TGG + Chr6:76747287-76747306 None:intergenic 25.0%
! TACATGAAAAATACAACATC+AGG + Chr6:76745004-76745023 None:intergenic 25.0%
! TATTAATATAGCTTAGGTGA+TGG - Chr6:76747213-76747232 MsG0680033932.01.T01:intron 25.0%
! TTAAACTAATTGACCCAAAA+TGG + Chr6:76744698-76744717 None:intergenic 25.0%
! TTGAGCTATTAATATAGCTT+AGG - Chr6:76747207-76747226 MsG0680033932.01.T01:intron 25.0%
! TTGATTCATGACTTAAAACT+AGG - Chr6:76744285-76744304 MsG0680033932.01.T01:intron 25.0%
! TTGTATACCATTGTTTATAC+TGG - Chr6:76743771-76743790 MsG0680033932.01.T01:three_prime_UTR 25.0%
! TTTCTTTACCTTAATGACAT+CGG + Chr6:76744871-76744890 None:intergenic 25.0%
!! ATACGATCCAAATTTAAGAT+TGG - Chr6:76744788-76744807 MsG0680033932.01.T01:intron 25.0%
!! CAAATTTAAGATTGGCATTA+TGG - Chr6:76744796-76744815 MsG0680033932.01.T01:intron 25.0%
!! CCAAAGATTATAATTTTGGA+AGG + Chr6:76747259-76747278 None:intergenic 25.0%
!! CTTATCATAAAGTGTTTATG+TGG + Chr6:76745836-76745855 None:intergenic 25.0%
!! TTAAGATTGAAAGGTATGAT+CGG + Chr6:76745294-76745313 None:intergenic 25.0%
!! TTGTATTTTGCAAATGTATG+TGG - Chr6:76747059-76747078 MsG0680033932.01.T01:CDS 25.0%
!! TTTAGGATTGAAATCGTATA+CGG - Chr6:76743698-76743717 MsG0680033932.01.T01:three_prime_UTR 25.0%
!!! GATGATAACATTGATTTTTC+AGG - Chr6:76744662-76744681 MsG0680033932.01.T01:intron 25.0%
!!! TTTTTTCTAGTGAGCTTAAT+CGG - Chr6:76744931-76744950 MsG0680033932.01.T01:intron 25.0%
!!! TTTTTTTTTTTGTGGTTGCT+GGG + Chr6:76746142-76746161 None:intergenic 25.0%
AAATTATCATTCGCTGTTCA+GGG - Chr6:76746635-76746654 MsG0680033932.01.T01:intron 30.0%
AACAAACCTGAAAAATACTC+GGG + Chr6:76746037-76746056 None:intergenic 30.0%
AAGATCTGATAACTCATAGT+AGG + Chr6:76745325-76745344 None:intergenic 30.0%
AATTTGGCACCACAATTATT+AGG + Chr6:76745904-76745923 None:intergenic 30.0%
ACGATTTCAATCCTAAATGT+TGG + Chr6:76743695-76743714 None:intergenic 30.0%
AGATGAATTTGGTTACATGT+TGG - Chr6:76744390-76744409 MsG0680033932.01.T01:intron 30.0%
AGCAGAAAATTATTGTAACC+GGG + Chr6:76746295-76746314 None:intergenic 30.0%
ATGACTTAAAACTAGGTCTT+TGG - Chr6:76744292-76744311 MsG0680033932.01.T01:intron 30.0%
ATTTGTCGTTGTGATGTATA+AGG - Chr6:76746917-76746936 MsG0680033932.01.T01:intron 30.0%
CATGTATATACAGATAAGTG+AGG - Chr6:76745018-76745037 MsG0680033932.01.T01:CDS 30.0%
GAATGGCATAATGAGTATAT+GGG - Chr6:76744839-76744858 MsG0680033932.01.T01:intron 30.0%
GACAATGCATAATATATGCA+AGG - Chr6:76746102-76746121 MsG0680033932.01.T01:intron 30.0%
GCTTTAACCAGTATAAACAA+TGG + Chr6:76743781-76743800 None:intergenic 30.0%
GGGTTTAAAAATTTAAACCC+CGG + Chr6:76746274-76746293 None:intergenic 30.0%
GTAAAAAATTATCGTAACCG+GGG - Chr6:76746357-76746376 MsG0680033932.01.T01:intron 30.0%
TAAATTATCATTCGCTGTTC+AGG - Chr6:76746634-76746653 MsG0680033932.01.T01:intron 30.0%
TCTTAGAAAAGAAGACCATA+AGG - Chr6:76743830-76743849 MsG0680033932.01.T01:three_prime_UTR 30.0%
TGAGAGTAGCAATAAAATCA+AGG - Chr6:76747357-76747376 MsG0680033932.01.T01:CDS 30.0%
TGATAGAGATCTCTTAAAGA+AGG - Chr6:76744573-76744592 MsG0680033932.01.T01:intron 30.0%
TTATACATCACAACGACAAA+TGG + Chr6:76746918-76746937 None:intergenic 30.0%
TTTGTCGTTGTGATGTATAA+GGG - Chr6:76746918-76746937 MsG0680033932.01.T01:intron 30.0%
! AGGCTACAATTTTCCATTTT+GGG - Chr6:76744682-76744701 MsG0680033932.01.T01:intron 30.0%
! GGGTTTAAATTTTTAAACCC+CGG - Chr6:76746274-76746293 MsG0680033932.01.T01:CDS 30.0%
! TATTCATGGTTTTTAAACCC+CGG + Chr6:76746377-76746396 None:intergenic 30.0%
!! GAGATTACATTTTTGACTGA+AGG - Chr6:76745043-76745062 MsG0680033932.01.T01:CDS 30.0%
!! GCTATTTTCATAAGCTATCT+TGG + Chr6:76745757-76745776 None:intergenic 30.0%
!!! GTTTTTTTTTTTGTGGTTGC+TGG + Chr6:76746143-76746162 None:intergenic 30.0%
AATTCCTTCACTTCCTACAA+TGG - Chr6:76747109-76747128 MsG0680033932.01.T01:CDS 35.0%
AATTGTTACTGAAGGGTCTA+AGG - Chr6:76745129-76745148 MsG0680033932.01.T01:intron 35.0%
ACCTCTTTCTGTAACTGTTA+TGG + Chr6:76747087-76747106 None:intergenic 35.0%
AGATATACCAACAGCAACAT+TGG + Chr6:76743973-76743992 None:intergenic 35.0%
ATGAAGATTGCCAAAAGTTC+TGG - Chr6:76744753-76744772 MsG0680033932.01.T01:intron 35.0%
ATTGTTACTGAAGGGTCTAA+GGG - Chr6:76745130-76745149 MsG0680033932.01.T01:intron 35.0%
CAAACATGGCCTAATAATTG+TGG - Chr6:76745892-76745911 MsG0680033932.01.T01:CDS 35.0%
CACAATTATTAGGCCATGTT+TGG + Chr6:76745894-76745913 None:intergenic 35.0%
CAGGTATCTACAACCTTATA+AGG + Chr6:76745427-76745446 None:intergenic 35.0%
CGAGGACAGTAAAATATTCA+TGG + Chr6:76746391-76746410 None:intergenic 35.0%
CGTACGAAAAAGTCCTTATA+AGG - Chr6:76745411-76745430 MsG0680033932.01.T01:intron 35.0%
GAAATTCCTGCACAGAAATT+TGG + Chr6:76745920-76745939 None:intergenic 35.0%
GACTGACACTTAAGATTGAA+AGG + Chr6:76745303-76745322 None:intergenic 35.0%
GAGCTCCTTCAAAAACTAAA+AGG + Chr6:76747331-76747350 None:intergenic 35.0%
GATCTTCTTACTGATCTCTT+TGG - Chr6:76746452-76746471 MsG0680033932.01.T01:CDS 35.0%
GCAGAAAATTATTGTAACCG+GGG + Chr6:76746294-76746313 None:intergenic 35.0%
GCATACATTCATGTACAAGT+GGG + Chr6:76746834-76746853 None:intergenic 35.0%
GCTGAAGGAAATAGAGATAA+AGG - Chr6:76746574-76746593 MsG0680033932.01.T01:CDS 35.0%
GCTGTTGGTATATCTAGATT+GGG - Chr6:76743978-76743997 MsG0680033932.01.T01:CDS 35.0%
GGAATGGCATAATGAGTATA+TGG - Chr6:76744838-76744857 MsG0680033932.01.T01:intron 35.0%
GGGTTGTAGATATTACACAA+AGG - Chr6:76745150-76745169 MsG0680033932.01.T01:intron 35.0%
GTATATACAGATAAGTGAGG+AGG - Chr6:76745021-76745040 MsG0680033932.01.T01:CDS 35.0%
GTTTCAGGGAAAGTTAAATG+AGG - Chr6:76745446-76745465 MsG0680033932.01.T01:intron 35.0%
TAACCTATGAAACACTGACA+CGG + Chr6:76743733-76743752 None:intergenic 35.0%
TCTTATATTACAGCTGCTTC+TGG + Chr6:76747155-76747174 None:intergenic 35.0%
TGAGCAAATACAGAGAGATA+AGG + Chr6:76746184-76746203 None:intergenic 35.0%
TGCATAATATATGCAAGGTC+CGG - Chr6:76746107-76746126 MsG0680033932.01.T01:intron 35.0%
TGCATACATTCATGTACAAG+TGG + Chr6:76746835-76746854 None:intergenic 35.0%
TGCTGTTGGTATATCTAGAT+TGG - Chr6:76743977-76743996 MsG0680033932.01.T01:CDS 35.0%
TTAAGCTGTCTATCCAAACA+TGG - Chr6:76745878-76745897 MsG0680033932.01.T01:CDS 35.0%
! CAGGCTACAATTTTCCATTT+TGG - Chr6:76744681-76744700 MsG0680033932.01.T01:intron 35.0%
! CATTTAACTTTCCCTGAAAC+AGG + Chr6:76745446-76745465 None:intergenic 35.0%
! TTCTCTTTTTCTTTTCGACG+AGG + Chr6:76746409-76746428 None:intergenic 35.0%
!! AATGTAATGGACATGTGTCA+GGG - Chr6:76745240-76745259 MsG0680033932.01.T01:intron 35.0%
!! TTCATCTGCTTTTATAGGCA+AGG - Chr6:76744004-76744023 MsG0680033932.01.T01:intron 35.0%
AGGTTGTAGATACCTGTTTC+AGG - Chr6:76745431-76745450 MsG0680033932.01.T01:intron 40.0%
ATACCGTGTCAGTGTTTCAT+AGG - Chr6:76743727-76743746 MsG0680033932.01.T01:three_prime_UTR 40.0%
ATAGACTTTGTGCCAACTGT+GGG - Chr6:76743891-76743910 MsG0680033932.01.T01:CDS 40.0%
ATTTCTGTGCAGGAATTTCG+AGG - Chr6:76745921-76745940 MsG0680033932.01.T01:CDS 40.0%
CAGAACAATGTCGATACTTC+TGG - Chr6:76744427-76744446 MsG0680033932.01.T01:intron 40.0%
CATAATATATGCAAGGTCCG+GGG - Chr6:76746109-76746128 MsG0680033932.01.T01:intron 40.0%
CCATAAGGAAACAAGTTCAC+TGG - Chr6:76743845-76743864 MsG0680033932.01.T01:exon 40.0%
GAAATGTGGTTCAAGACCTA+TGG - Chr6:76744624-76744643 MsG0680033932.01.T01:intron 40.0%
GCATAATATATGCAAGGTCC+GGG - Chr6:76746108-76746127 MsG0680033932.01.T01:intron 40.0%
GCCATAACAGTTACAGAAAG+AGG - Chr6:76747083-76747102 MsG0680033932.01.T01:CDS 40.0%
GCTAAGCCAAACAACATCTT+AGG + Chr6:76745360-76745379 None:intergenic 40.0%
GGTTGTAGATACCTGTTTCA+GGG - Chr6:76745432-76745451 MsG0680033932.01.T01:intron 40.0%
GTCTATCAACATTTCCCCAA+AGG + Chr6:76743877-76743896 None:intergenic 40.0%
GTTAAACCTGTTGACACAAC+CGG - Chr6:76745963-76745982 MsG0680033932.01.T01:CDS 40.0%
GTTGGTATATCTAGATTGGG+AGG - Chr6:76743981-76744000 MsG0680033932.01.T01:intron 40.0%
TGCAAAACAGCTTCCATTGT+AGG + Chr6:76747125-76747144 None:intergenic 40.0%
TTAAACGCACATATGGGACA+AGG + Chr6:76745568-76745587 None:intergenic 40.0%
TTTGTCACACTTAGATCCGA+CGG - Chr6:76744487-76744506 MsG0680033932.01.T01:intron 40.0%
! AAGGTGCCTAAGATGTTGTT+TGG - Chr6:76745351-76745370 MsG0680033932.01.T01:intron 40.0%
! ACGAGTAAATGCGTGTCATT+TGG - Chr6:76746691-76746710 MsG0680033932.01.T01:intron 40.0%
! AGTTCACTGGTTGTTTCCTT+TGG - Chr6:76743858-76743877 MsG0680033932.01.T01:exon 40.0%
! CCAGTGAACTTGTTTCCTTA+TGG + Chr6:76743848-76743867 None:intergenic 40.0%
! GAGAATGCAACCAGAACTTT+TGG + Chr6:76744766-76744785 None:intergenic 40.0%
! GTTCACTGGTTGTTTCCTTT+GGG - Chr6:76743859-76743878 MsG0680033932.01.T01:exon 40.0%
! TAGGTTGGAGCAGATGAATT+TGG - Chr6:76744379-76744398 MsG0680033932.01.T01:intron 40.0%
! TGAAGAAGCATATCAGCACT+TGG + Chr6:76744541-76744560 None:intergenic 40.0%
! TTCACTGGTTGTTTCCTTTG+GGG - Chr6:76743860-76743879 MsG0680033932.01.T01:exon 40.0%
! TTCTGACCCGAGTATTTTTC+AGG - Chr6:76746028-76746047 MsG0680033932.01.T01:intron 40.0%
!! GAATGTAATGGACATGTGTC+AGG - Chr6:76745239-76745258 MsG0680033932.01.T01:intron 40.0%
!! GGAGGTTCATCTGCTTTTAT+AGG - Chr6:76743999-76744018 MsG0680033932.01.T01:intron 40.0%
!!! AGCGTGAGTTCTTGTTTTTC+CGG - Chr6:76744512-76744531 MsG0680033932.01.T01:intron 40.0%
AACAAATGCATCACCAGCAC+CGG + Chr6:76745985-76746004 None:intergenic 45.0%
AGCTAATGCAGTCCTTGCAT+TGG + Chr6:76744467-76744486 None:intergenic 45.0%
ATACCAACAGCAACATTGGC+AGG + Chr6:76743969-76743988 None:intergenic 45.0%
ATGTCGATACTTCTGGCATG+AGG - Chr6:76744434-76744453 MsG0680033932.01.T01:intron 45.0%
CAAGCAACGTCTTCAACACA+AGG + Chr6:76746482-76746501 None:intergenic 45.0%
CCAGGAGATTTCTTGAAAGC+CGG + Chr6:76743939-76743958 None:intergenic 45.0%
GATAGACTTTGTGCCAACTG+TGG - Chr6:76743890-76743909 MsG0680033932.01.T01:CDS 45.0%
GCATATCAGCACTTGGATTC+CGG + Chr6:76744534-76744553 None:intergenic 45.0%
GCTTCCATTGTAGGAAGTGA+AGG + Chr6:76747116-76747135 None:intergenic 45.0%
GCTTTCAAGAAATCTCCTGG+TGG - Chr6:76743939-76743958 MsG0680033932.01.T01:CDS 45.0%
GGATCAAGCCGATGTCATTA+AGG - Chr6:76744860-76744879 MsG0680033932.01.T01:intron 45.0%
TACCAACTGAGCTAAGCTCA+CGG + Chr6:76746080-76746099 None:intergenic 45.0%
TACTAGCGACGCCAACATTT+AGG - Chr6:76743681-76743700 MsG0680033932.01.T01:three_prime_UTR 45.0%
TGAGGTTTGATGCCAATGCA+AGG - Chr6:76744452-76744471 MsG0680033932.01.T01:intron 45.0%
! GCTGGTGATGCATTTGTTAG+TGG - Chr6:76745987-76746006 MsG0680033932.01.T01:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+AGG - Chr6:76746080-76746099 MsG0680033932.01.T01:intron 45.0%
!! GCAATGTGTGCTGATTTGCA+TGG + Chr6:76744729-76744748 None:intergenic 45.0%
!!! AAAAAAAATAATATTTTTAC+TGG - Chr6:76746533-76746552 MsG0680033932.01.T01:CDS 5.0%
!!! TTATATTATATATATTTTCT+TGG - Chr6:76747009-76747028 MsG0680033932.01.T01:CDS 5.0%
ACCAACTGAGCTAAGCTCAC+GGG + Chr6:76746079-76746098 None:intergenic 50.0%
CCGGCTTTCAAGAAATCTCC+TGG - Chr6:76743936-76743955 MsG0680033932.01.T01:CDS 50.0%
CTGGGATGAACTCTTGCTGA+AGG - Chr6:76746559-76746578 MsG0680033932.01.T01:CDS 50.0%
TATGCCATTCCAAGCAGCCT+CGG + Chr6:76744829-76744848 None:intergenic 50.0%
TGTGCAGGAATTTCGAGGCA+GGG - Chr6:76745926-76745945 MsG0680033932.01.T01:CDS 50.0%
TTTCTTGAAAGCCGGTGCCT+CGG + Chr6:76743931-76743950 None:intergenic 50.0%
! GTGGTGCCAAATTTCTGTGC+AGG - Chr6:76745911-76745930 MsG0680033932.01.T01:CDS 50.0%
CAAGAACTCACGCTCACCGT+CGG + Chr6:76744506-76744525 None:intergenic 55.0%
CAGGAATTTCGAGGCAGGGT+TGG - Chr6:76745930-76745949 MsG0680033932.01.T01:CDS 55.0%
CCAACTGAGCTAAGCTCACG+GGG + Chr6:76746078-76746097 None:intergenic 55.0%
CTGTGCAGGAATTTCGAGGC+AGG - Chr6:76745925-76745944 MsG0680033932.01.T01:CDS 55.0%
GAATTTCGAGGCAGGGTTGG+AGG - Chr6:76745933-76745952 MsG0680033932.01.T01:CDS 55.0%
GACTTTGTGCCAACTGTGGG+AGG - Chr6:76743894-76743913 MsG0680033932.01.T01:CDS 55.0%
GCTCCTGCCAATGTTGCTGT+TGG - Chr6:76743963-76743982 MsG0680033932.01.T01:CDS 55.0%
ACATTGGCAGGAGCTCCACC+AGG + Chr6:76743957-76743976 None:intergenic 60.0%
AGCGACACTCCTCCCACAGT+TGG + Chr6:76743906-76743925 None:intergenic 60.0%
CCCCGTGAGCTTAGCTCAGT+TGG - Chr6:76746075-76746094 MsG0680033932.01.T01:intron 60.0%
CCTGTTGACACAACCGGTGC+TGG - Chr6:76745969-76745988 MsG0680033932.01.T01:CDS 60.0%
GGTTGCTGGGATTCGAACCC+CGG + Chr6:76746129-76746148 None:intergenic 60.0%
GTGCAGTGTCCGACACGACA+CGG + Chr6:76745272-76745291 None:intergenic 60.0%
TCTGCCGAGGCTGCTTGGAA+TGG - Chr6:76744822-76744841 MsG0680033932.01.T01:intron 60.0%
TTCCAAGCAGCCTCGGCAGA+CGG + Chr6:76744822-76744841 None:intergenic 60.0%
!! CCAGCACCGGTTGTGTCAAC+AGG + Chr6:76745972-76745991 None:intergenic 60.0%
GGCATTATGGCCGTCTGCCG+AGG - Chr6:76744809-76744828 MsG0680033932.01.T01:intron 65.0%
GGGTCAGTGCCGTGTCGTGT+CGG - Chr6:76745260-76745279 MsG0680033932.01.T01:intron 65.0%
! AGTGTCGCTCGCCGAGGCAC+CGG - Chr6:76743917-76743936 MsG0680033932.01.T01:CDS 70.0%
GGCCGTCTGCCGAGGCTGCT+TGG - Chr6:76744817-76744836 MsG0680033932.01.T01:intron 75.0%
!! GGGAGGAGTGTCGCTCGCCG+AGG - Chr6:76743911-76743930 MsG0680033932.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 76743638 76747395 76743638 ID=MsG0680033932.01;Name=MsG0680033932.01
Chr6 mRNA 76743638 76747395 76743638 ID=MsG0680033932.01.T01;Parent=MsG0680033932.01;Name=MsG0680033932.01.T01;_AED=0.45;_eAED=0.53;_QI=0|0.57|0.75|1|1|1|8|223|406
Chr6 exon 76747314 76747395 76747314 ID=MsG0680033932.01.T01:exon:2010;Parent=MsG0680033932.01.T01
Chr6 exon 76747008 76747216 76747008 ID=MsG0680033932.01.T01:exon:2011;Parent=MsG0680033932.01.T01
Chr6 exon 76746439 76746651 76746439 ID=MsG0680033932.01.T01:exon:2012;Parent=MsG0680033932.01.T01
Chr6 exon 76746152 76746349 76746152 ID=MsG0680033932.01.T01:exon:2013;Parent=MsG0680033932.01.T01
Chr6 exon 76745862 76746002 76745862 ID=MsG0680033932.01.T01:exon:2014;Parent=MsG0680033932.01.T01
Chr6 exon 76745650 76745772 76745650 ID=MsG0680033932.01.T01:exon:2015;Parent=MsG0680033932.01.T01
Chr6 exon 76744984 76745100 76744984 ID=MsG0680033932.01.T01:exon:2016;Parent=MsG0680033932.01.T01
Chr6 exon 76743638 76743998 76743638 ID=MsG0680033932.01.T01:exon:2017;Parent=MsG0680033932.01.T01
Chr6 CDS 76747314 76747395 76747314 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76747008 76747216 76747008 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76746439 76746651 76746439 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76746152 76746349 76746152 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76745862 76746002 76745862 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76745650 76745772 76745650 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76744984 76745100 76744984 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 CDS 76743861 76743998 76743861 ID=MsG0680033932.01.T01:cds;Parent=MsG0680033932.01.T01
Chr6 three_prime_UTR 76743638 76743860 76743638 ID=MsG0680033932.01.T01:three_prime_utr;Parent=MsG0680033932.01.T01
Gene Sequence

>MsG0680033932.01.T01

ATGAAGTGTTCAATATTGACTGAATTCTCTGCAATTAGATTGTTACTAGCGACGCCAACATTTAGGATTGAAATCGTATACGTTGAATCTAATGATCTTAGAAAAGAAGACCATAAGGAAACAAGTTCACTGGTTGTTTCCTTTGGGGAAATGTTGATAGACTTTGTGCCAACTGTGGGAGGAGTGTCGCTCGCCGAGGCACCGGCTTTCAAGAAATCTCCTGGTGGAGCTCCTGCCAATGTTGCTGTTGGTATATCTAGATTGGGAGGTTCATCTGCTTTTATAGGCAAGGTTGGAGCAGATGAATTTGGTTACATGTTGGCTGATATTTTAAAGCAGAACAATGTCGATACTTCTGGCATGAGGTTTGATGCCAATGCAAGGACTGCATTAGCTTTTGTCACACTTAGATCCGACGGTGAGCGTGAGTTCTTGTTTTTCCGGAATCCAAGTGCTGATATGCTTCTTCACGAATCAGAACTTGATAGAGATCTCTTAAAGAAGGCTACAATTTTCCATTTTGGGTCAATTAGTTTAATTGATGATCCATGCAAATCAGCACACATTGCTGCTATGAAGATTGCCAAAAGTTCTGGTTGCATTCTCTCATACGATCCAAATTTAAGATTGGCATTATGGCCGTCTGCCGAGGCTGCTTGGAATGGCATAATGAGTATATGGGATCAAGCCGATGTCATTAAGATAAGTGAGGAGGAGATTACATTTTTGACTGAAGGTGATGATCCTAATGATGATGATGTTGTTTTGAAGAAACTTTTTCATCGTAATCTCAAACTCTTAATTGTTACTGAAGGGTCTAAGGGTTGTAGATATTACACAAAGGGTCAGTGCCGTGTCGTGTCGGACACTGCACCGATCATACCTTTCAATCTTAAGTGTCAGTCCTACTATGAGTTATCAGATCTTATAACAAAGGTGCCTAAGATGTTGTTTGGCTTAGCTTTTGAATTTCGAGGCAGGGTTGGAGGTGTTAAAGTTAAACCTGTTGACACAACCGGTGCTGGTGATGCATTTGTTAGTGGATTTCTGTACAGTATAGCTTCTGACCCGAGTATTTTTCAGGATGAGAAAAGTCTGAAGAAAGCTTTGTATTTTGCAAATGTATGTGGTGCCATAACAGTTACAGAAAGAGGTGCAATTCCTTCACTTCCTACAATGGAAGCTGTTTTGCAATTCTTACCAGAAGCAGCTGTAATATAA
Protein sequence

>MsG0680033932.01.T01

MKCSILTEFSAIRLLLATPTFRIEIVYVESNDLRKEDHKETSSLVVSFGEMLIDFVPTVGGVSLAEAPAFKKSPGGAPANVAVGISRLGGSSAFIGKVGADEFGYMLADILKQNNVDTSGMRFDANARTALAFVTLRSDGEREFLFFRNPSADMLLHESELDRDLLKKATIFHFGSISLIDDPCKSAHIAAMKIAKSSGCILSYDPNLRLALWPSAEAAWNGIMSIWDQADVIKISEEEITFLTEGDDPNDDDVVLKKLFHRNLKLLIVTEGSKGCRYYTKGQCRVVSDTAPIIPFNLKCQSYYELSDLITKVPKMLFGLAFEFRGRVGGVKVKPVDTTGAGDAFVSGFLYSIASDPSIFQDEKSLKKALYFANVCGAITVTERGAIPSLPTMEAVLQFLPEAAVI*