Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034058.01.T01 | RHN60756.1 | 85.955 | 178 | 22 | 1 | 41 | 215 | 11 | 188 | 7.28E-100 | 301 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034058.01.T01 | A0A396HIN3 | 85.714 | 175 | 25 | 0 | 41 | 215 | 125 | 299 | 8.53e-98 | 305 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580027688.01 | MsG0680034058.01 | 0.805233 | 1.473912e-49 | 7.756725e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034058.01.T01 | MTR_1g057850 | 78.512 | 121 | 26 | 0 | 80 | 200 | 54 | 174 | 2.59e-58 | 194 |
| MsG0680034058.01.T01 | MTR_1g052045 | 58.993 | 139 | 21 | 2 | 77 | 215 | 94 | 196 | 5.64e-45 | 151 |
| MsG0680034058.01.T01 | MTR_5g073300 | 46.226 | 106 | 37 | 1 | 52 | 157 | 133 | 218 | 2.12e-21 | 89.0 |
| MsG0680034058.01.T01 | MTR_1g054790 | 79.070 | 43 | 9 | 0 | 163 | 205 | 18 | 60 | 1.43e-16 | 73.6 |
| MsG0680034058.01.T01 | MTR_0040s0060 | 36.283 | 113 | 28 | 1 | 50 | 162 | 1 | 69 | 1.21e-15 | 69.3 |
| MsG0680034058.01.T01 | MTR_3g049870 | 68.889 | 45 | 14 | 0 | 56 | 100 | 148 | 192 | 6.17e-15 | 72.0 |
| MsG0680034058.01.T01 | MTR_4g022650 | 73.684 | 38 | 10 | 0 | 44 | 81 | 19 | 56 | 5.80e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCAATAAGCTTCAGTATT+TGG | 0.149875 | 6:-79811658 | MsG0680034058.01.T01:CDS |
| TGAACAGCATTGTTCCTTTC+AGG | 0.166557 | 6:+79812456 | None:intergenic |
| AGCCAAATACTGAAGCTTAT+TGG | 0.251450 | 6:+79811656 | None:intergenic |
| TTAGATGACTTTCCTTTAGA+TGG | 0.251924 | 6:+79811762 | None:intergenic |
| GGAGATCCTGAAGAATTTCT+TGG | 0.352555 | 6:-79812387 | MsG0680034058.01.T01:intron |
| GATTTCTCCTTCTGAAGATC+AGG | 0.372143 | 6:+79811522 | None:intergenic |
| TTTCCACCAAGAAATTCTTC+AGG | 0.397294 | 6:+79812381 | None:intergenic |
| CTCCAAGAAGGAAGATCAAA+AGG | 0.410182 | 6:-79811589 | MsG0680034058.01.T01:CDS |
| TCAGGATCTCCATTGAACTT+TGG | 0.416263 | 6:+79812399 | None:intergenic |
| GCATTGTTCCTTTCAGGAAT+GGG | 0.435941 | 6:+79812462 | None:intergenic |
| TGAGAAGCTTACCCTCTAGA+TGG | 0.439414 | 6:-79811923 | MsG0680034058.01.T01:CDS |
| TCTAATGCTATTCCATCTAA+AGG | 0.458006 | 6:-79811774 | MsG0680034058.01.T01:CDS |
| TGCTTCAATTGTAAGAAGCC+TGG | 0.463895 | 6:-79811564 | MsG0680034058.01.T01:CDS |
| AGCATTGTTCCTTTCAGGAA+TGG | 0.477303 | 6:+79812461 | None:intergenic |
| GGCAAAGCTCCAAAGTTCAA+TGG | 0.496770 | 6:-79812408 | MsG0680034058.01.T01:CDS |
| GTATTTGAAGTCCAGAGACT+AGG | 0.502057 | 6:+79811988 | None:intergenic |
| ACCTAAGGTGACTGCTATTG+AGG | 0.503374 | 6:-79811898 | MsG0680034058.01.T01:CDS |
| AAGAAGTTCTTGAGCAGAAG+AGG | 0.503997 | 6:-79811624 | MsG0680034058.01.T01:CDS |
| AGATTTCAGACCCTAGTCTC+TGG | 0.512253 | 6:-79811999 | MsG0680034058.01.T01:CDS |
| GGACTTCAAATACTGAAGAA+AGG | 0.514490 | 6:-79811978 | MsG0680034058.01.T01:CDS |
| TCCTCAATAGCAGTCACCTT+AGG | 0.515767 | 6:+79811897 | None:intergenic |
| AGATCTTGTGAGTTCTCTCA+AGG | 0.528917 | 6:-79811844 | MsG0680034058.01.T01:CDS |
| TGAAGACCCTTCTGAGAAGA+TGG | 0.535262 | 6:-79811688 | MsG0680034058.01.T01:CDS |
| CAATCAGCAATGAAATGTCC+AGG | 0.546593 | 6:+79811546 | None:intergenic |
| AGCATAGCCATCTTCTCAGA+AGG | 0.550818 | 6:+79811681 | None:intergenic |
| TATTTGAAGTCCAGAGACTA+GGG | 0.556636 | 6:+79811989 | None:intergenic |
| TCTTCATCAGAATCTCCATC+AGG | 0.559331 | 6:+79811705 | None:intergenic |
| AGCAGAAGAGGTAGTTACAA+AGG | 0.564954 | 6:-79811612 | MsG0680034058.01.T01:CDS |
| ATTTCTCCTTCTGAAGATCA+GGG | 0.570852 | 6:+79811523 | None:intergenic |
| TCTGAAGAAGAATCACCTGA+TGG | 0.577900 | 6:-79811720 | MsG0680034058.01.T01:CDS |
| CACCTTAGGTCTCCATCTAG+AGG | 0.580704 | 6:+79811911 | None:intergenic |
| TAGTTACAAAGGCTCCAAGA+AGG | 0.591637 | 6:-79811601 | MsG0680034058.01.T01:CDS |
| GATGATAAGAAATATGACTC+AGG | 0.596155 | 6:-79812429 | MsG0680034058.01.T01:CDS |
| TGATTGCCCTGATCTTCAGA+AGG | 0.597637 | 6:-79811529 | MsG0680034058.01.T01:CDS |
| GTGAAGTTCCCATTCCTGAA+AGG | 0.601957 | 6:-79812470 | MsG0680034058.01.T01:CDS |
| TCCAAGAAGGAAGATCAAAA+GGG | 0.608136 | 6:-79811588 | MsG0680034058.01.T01:CDS |
| ACCCTCTAGATGGAGACCTA+AGG | 0.619798 | 6:-79811913 | MsG0680034058.01.T01:CDS |
| ACCTTAGGTCTCCATCTAGA+GGG | 0.626220 | 6:+79811912 | None:intergenic |
| GCATAGCCATCTTCTCAGAA+GGG | 0.628545 | 6:+79811682 | None:intergenic |
| AGGTGATGATGAAAGTATTG+AGG | 0.631082 | 6:-79812033 | MsG0680034058.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTATGAAGGTAGTAAGA+AGG | - | Chr6:79811894-79811913 | MsG0680034058.01.T01:CDS | 30.0% | |
| AATATTCTTGAAGATGGTGT+TGG | - | Chr6:79811690-79811709 | MsG0680034058.01.T01:CDS | 30.0% | |
| CATATGTACAGTTACATCAT+GGG | - | Chr6:79811648-79811667 | MsG0680034058.01.T01:CDS | 30.0% | |
| GATGATAAGAAATATGACTC+AGG | - | Chr6:79811575-79811594 | MsG0680034058.01.T01:CDS | 30.0% | |
| TCTAATGCTATTCCATCTAA+AGG | - | Chr6:79812230-79812249 | MsG0680034058.01.T01:intron | 30.0% | |
| TTAGATGACTTTCCTTTAGA+TGG | + | Chr6:79812245-79812264 | None:intergenic | 30.0% | |
| TTCCAATAAGCTTCAGTATT+TGG | - | Chr6:79812346-79812365 | MsG0680034058.01.T01:intron | 30.0% | |
| TTCTTACTACCTTCATAGTT+TGG | + | Chr6:79811895-79811914 | None:intergenic | 30.0% | |
| ACATTGCCTTTGCATAGATT+TGG | + | Chr6:79811857-79811876 | None:intergenic | 35.0% | |
| AGCCAAATACTGAAGCTTAT+TGG | + | Chr6:79812351-79812370 | None:intergenic | 35.0% | |
| CCATATGTACAGTTACATCA+TGG | - | Chr6:79811647-79811666 | MsG0680034058.01.T01:CDS | 35.0% | |
| CCATGATGTAACTGTACATA+TGG | + | Chr6:79811650-79811669 | None:intergenic | 35.0% | |
| CTGTGGAATATTCTTGAAGA+TGG | - | Chr6:79811684-79811703 | MsG0680034058.01.T01:CDS | 35.0% | |
| GGACTTCAAATACTGAAGAA+AGG | - | Chr6:79812026-79812045 | MsG0680034058.01.T01:CDS | 35.0% | |
| GTATGAGTTGTTCAAGATGA+AGG | - | Chr6:79811951-79811970 | MsG0680034058.01.T01:CDS | 35.0% | |
| TCATCTTGAACAACTCATAC+TGG | + | Chr6:79811951-79811970 | None:intergenic | 35.0% | |
| TCCAAGAAGGAAGATCAAAA+GGG | - | Chr6:79812416-79812435 | MsG0680034058.01.T01:CDS | 35.0% | |
| TTTCCACCAAGAAATTCTTC+AGG | + | Chr6:79811626-79811645 | None:intergenic | 35.0% | |
| ! | ATTTCTCCTTCTGAAGATCA+GGG | + | Chr6:79812484-79812503 | None:intergenic | 35.0% |
| ! | TATTTGAAGTCCAGAGACTA+GGG | + | Chr6:79812018-79812037 | None:intergenic | 35.0% |
| !! | AGGTGATGATGAAAGTATTG+AGG | - | Chr6:79811971-79811990 | MsG0680034058.01.T01:CDS | 35.0% |
| AAGAAGTTCTTGAGCAGAAG+AGG | - | Chr6:79812380-79812399 | MsG0680034058.01.T01:intron | 40.0% | |
| AGATCTTGTGAGTTCTCTCA+AGG | - | Chr6:79812160-79812179 | MsG0680034058.01.T01:intron | 40.0% | |
| AGCAGAAGAGGTAGTTACAA+AGG | - | Chr6:79812392-79812411 | MsG0680034058.01.T01:CDS | 40.0% | |
| AGCATTGTTCCTTTCAGGAA+TGG | + | Chr6:79811546-79811565 | None:intergenic | 40.0% | |
| AGGTAGTAAGAAGGTTAGAG+AGG | - | Chr6:79811903-79811922 | MsG0680034058.01.T01:CDS | 40.0% | |
| ATCTTCATGTACTATGTGCG+AGG | + | Chr6:79811829-79811848 | None:intergenic | 40.0% | |
| CAATCAGCAATGAAATGTCC+AGG | + | Chr6:79812461-79812480 | None:intergenic | 40.0% | |
| CACTTTGTGCCAAACTATGA+AGG | - | Chr6:79811883-79811902 | MsG0680034058.01.T01:CDS | 40.0% | |
| CTCCAAGAAGGAAGATCAAA+AGG | - | Chr6:79812415-79812434 | MsG0680034058.01.T01:CDS | 40.0% | |
| CTTGTAAAGTTCTTCTGAGC+AGG | + | Chr6:79811768-79811787 | None:intergenic | 40.0% | |
| GACTTGGATCTTGATGAAGA+AGG | - | Chr6:79811714-79811733 | MsG0680034058.01.T01:CDS | 40.0% | |
| GATCCTGAAGAATTTCTTGG+TGG | - | Chr6:79811620-79811639 | MsG0680034058.01.T01:CDS | 40.0% | |
| GCATTGTTCCTTTCAGGAAT+GGG | + | Chr6:79811545-79811564 | None:intergenic | 40.0% | |
| GGAGATCCTGAAGAATTTCT+TGG | - | Chr6:79811617-79811636 | MsG0680034058.01.T01:CDS | 40.0% | |
| TAAGAAGGTTAGAGAGGCAA+AGG | - | Chr6:79811909-79811928 | MsG0680034058.01.T01:CDS | 40.0% | |
| TAGTTACAAAGGCTCCAAGA+AGG | - | Chr6:79812403-79812422 | MsG0680034058.01.T01:CDS | 40.0% | |
| TCAGGATCTCCATTGAACTT+TGG | + | Chr6:79811608-79811627 | None:intergenic | 40.0% | |
| TCTGAAGAAGAATCACCTGA+TGG | - | Chr6:79812284-79812303 | MsG0680034058.01.T01:intron | 40.0% | |
| TCTTCATCAGAATCTCCATC+AGG | + | Chr6:79812302-79812321 | None:intergenic | 40.0% | |
| TGAACAGCATTGTTCCTTTC+AGG | + | Chr6:79811551-79811570 | None:intergenic | 40.0% | |
| TGAGTGCCAAATCTATGCAA+AGG | - | Chr6:79811848-79811867 | MsG0680034058.01.T01:CDS | 40.0% | |
| TGCTTCAATTGTAAGAAGCC+TGG | - | Chr6:79812440-79812459 | MsG0680034058.01.T01:CDS | 40.0% | |
| TGGGATTAGATGATGAACTG+TGG | - | Chr6:79811667-79811686 | MsG0680034058.01.T01:CDS | 40.0% | |
| ! | ACCCTTTTGATCTTCCTTCT+TGG | + | Chr6:79812420-79812439 | None:intergenic | 40.0% |
| ! | GATTTCTCCTTCTGAAGATC+AGG | + | Chr6:79812485-79812504 | None:intergenic | 40.0% |
| ! | GTATTTGAAGTCCAGAGACT+AGG | + | Chr6:79812019-79812038 | None:intergenic | 40.0% |
| !! | AAGAAGCATCACAAGATGAG+AGG | - | Chr6:79811785-79811804 | MsG0680034058.01.T01:CDS | 40.0% |
| ACCTAAGGTGACTGCTATTG+AGG | - | Chr6:79812106-79812125 | MsG0680034058.01.T01:intron | 45.0% | |
| ACCTTAGGTCTCCATCTAGA+GGG | + | Chr6:79812095-79812114 | None:intergenic | 45.0% | |
| AGATTTCAGACCCTAGTCTC+TGG | - | Chr6:79812005-79812024 | MsG0680034058.01.T01:CDS | 45.0% | |
| AGCATAGCCATCTTCTCAGA+AGG | + | Chr6:79812326-79812345 | None:intergenic | 45.0% | |
| GCATAGCCATCTTCTCAGAA+GGG | + | Chr6:79812325-79812344 | None:intergenic | 45.0% | |
| GGCAAAGCTCCAAAGTTCAA+TGG | - | Chr6:79811596-79811615 | MsG0680034058.01.T01:CDS | 45.0% | |
| GTGAAGTTCCCATTCCTGAA+AGG | - | Chr6:79811534-79811553 | MsG0680034058.01.T01:CDS | 45.0% | |
| GTGCGAGGTATAATGCAACA+AGG | + | Chr6:79811814-79811833 | None:intergenic | 45.0% | |
| TCCTCAATAGCAGTCACCTT+AGG | + | Chr6:79812110-79812129 | None:intergenic | 45.0% | |
| TGAGAAGCTTACCCTCTAGA+TGG | - | Chr6:79812081-79812100 | MsG0680034058.01.T01:intron | 45.0% | |
| TGATTGCCCTGATCTTCAGA+AGG | - | Chr6:79812475-79812494 | MsG0680034058.01.T01:CDS | 45.0% | |
| ! | TGAAGACCCTTCTGAGAAGA+TGG | - | Chr6:79812316-79812335 | MsG0680034058.01.T01:intron | 45.0% |
| !! | TGAAGATGGTGTTGGTGACT+TGG | - | Chr6:79811698-79811717 | MsG0680034058.01.T01:CDS | 45.0% |
| ACCCTCTAGATGGAGACCTA+AGG | - | Chr6:79812091-79812110 | MsG0680034058.01.T01:intron | 50.0% | |
| CACCTTAGGTCTCCATCTAG+AGG | + | Chr6:79812096-79812115 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 79811515 | 79812511 | 79811515 | ID=MsG0680034058.01;Name=MsG0680034058.01 |
| Chr6 | mRNA | 79811515 | 79812511 | 79811515 | ID=MsG0680034058.01.T01;Parent=MsG0680034058.01;Name=MsG0680034058.01.T01;_AED=0.72;_eAED=0.72;_QI=0|0|0|0.5|1|1|2|0|220 |
| Chr6 | exon | 79812388 | 79812511 | 79812388 | ID=MsG0680034058.01.T01:exon:8471;Parent=MsG0680034058.01.T01 |
| Chr6 | exon | 79811515 | 79812053 | 79811515 | ID=MsG0680034058.01.T01:exon:8472;Parent=MsG0680034058.01.T01 |
| Chr6 | CDS | 79812388 | 79812511 | 79812388 | ID=MsG0680034058.01.T01:cds;Parent=MsG0680034058.01.T01 |
| Chr6 | CDS | 79811515 | 79812053 | 79811515 | ID=MsG0680034058.01.T01:cds;Parent=MsG0680034058.01.T01 |
| Gene Sequence |
| Protein sequence |
