AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034063.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034063.01.T02 MTR_2g059470 44.776 67 37 0 6 72 2 68 9.55e-16 65.5
MsG0680034063.01.T02 MTR_1g034430 45.070 71 39 0 2 72 93 163 1.36e-15 67.4
MsG0680034063.01.T02 MTR_0015s0120 47.143 70 36 1 4 72 293 362 9.76e-15 67.0
MsG0680034063.01.T02 MTR_4g008270 41.429 70 41 0 2 71 8 77 2.00e-14 62.4
MsG0680034063.01.T02 MTR_2g027020 46.377 69 33 1 2 70 26 90 2.00e-14 64.7
MsG0680034063.01.T02 MTR_6g453270 43.243 74 41 1 1 73 297 370 2.33e-14 66.2
MsG0680034063.01.T02 MTR_8g031800 44.286 70 39 0 2 71 29 98 2.49e-14 62.8
MsG0680034063.01.T02 MTR_5g049680 43.750 64 36 0 4 67 31 94 8.59e-14 61.2
MsG0680034063.01.T02 MTR_1g088650 45.455 66 36 0 2 67 84 149 9.07e-14 62.8
MsG0680034063.01.T02 MTR_6g016355 41.935 62 36 0 4 65 116 177 2.69e-13 61.6
MsG0680034063.01.T02 MTR_3g116210 41.791 67 39 0 4 70 97 163 4.54e-13 60.8
MsG0680034063.01.T02 MTR_7g063280 39.130 69 42 0 4 72 72 140 8.87e-13 60.1
MsG0680034063.01.T02 MTR_0093s0070 36.111 72 46 0 3 74 122 193 9.45e-13 60.5
MsG0680034063.01.T02 MTR_1g069475 43.284 67 38 0 4 70 164 230 1.31e-12 60.5
MsG0680034063.01.T02 MTR_5g083200 41.791 67 39 0 4 70 98 164 2.03e-12 59.3
MsG0680034063.01.T02 MTR_0002s1220 41.791 67 39 0 4 70 98 164 2.03e-12 59.3
MsG0680034063.01.T02 MTR_1g074090 40.000 65 39 0 3 67 80 144 2.76e-12 58.2
MsG0680034063.01.T02 MTR_3g111390 42.029 69 40 0 2 70 96 164 2.90e-12 58.9
MsG0680034063.01.T02 MTR_1g070820 42.029 69 40 0 2 70 96 164 2.90e-12 58.9
MsG0680034063.01.T02 MTR_8g079540 44.286 70 39 0 3 72 47 116 3.20e-12 57.4
MsG0680034063.01.T02 MTR_1g074240 43.103 58 33 0 6 63 2 59 3.93e-12 55.8
MsG0680034063.01.T02 MTR_6g016265 40.000 70 42 0 2 71 37 106 5.19e-12 57.0
MsG0680034063.01.T02 MTR_5g036320 41.791 67 39 0 4 70 79 145 8.10e-12 57.0
MsG0680034063.01.T02 MTR_5g075610 46.154 52 28 0 10 61 1 52 1.21e-11 55.5
MsG0680034063.01.T02 MTR_1g045340 40.580 69 41 0 2 70 82 150 1.28e-11 57.0
MsG0680034063.01.T02 MTR_5g034450 38.462 65 40 0 4 68 49 113 1.46e-11 55.5
MsG0680034063.01.T02 MTR_6g034915 40.625 64 38 0 5 68 56 119 3.69e-11 55.8
MsG0680034063.01.T02 MTR_1g050488 40.299 67 40 0 4 70 98 164 4.86e-11 55.1
MsG0680034063.01.T02 MTR_7g053400 48.276 58 30 0 8 65 179 236 4.91e-11 56.2
MsG0680034063.01.T02 MTR_5g085210 39.130 69 42 0 4 72 119 187 9.14e-11 55.5
MsG0680034063.01.T01 MTR_2g027020 45.000 140 73 1 2 141 26 161 7.00e-35 120
MsG0680034063.01.T01 MTR_1g088650 43.396 106 60 0 2 107 84 189 5.93e-23 89.7
MsG0680034063.01.T01 MTR_8g031080 39.855 138 57 1 2 139 287 398 3.91e-21 88.2
MsG0680034063.01.T01 MTR_6g034915 36.765 136 69 1 5 140 56 174 1.29e-20 83.2
MsG0680034063.01.T01 MTR_7g063280 36.538 104 66 0 4 107 72 175 1.41e-20 83.2
MsG0680034063.01.T01 MTR_5g075610 41.837 98 55 1 10 107 1 96 1.74e-20 80.9
MsG0680034063.01.T01 MTR_1g034430 43.820 89 50 0 2 90 93 181 9.72e-20 80.9
MsG0680034063.01.T01 MTR_4g029360 41.584 101 59 0 4 104 127 227 2.09e-19 81.3
MsG0680034063.01.T01 MTR_6g453270 43.902 82 46 0 1 82 297 378 4.14e-18 79.7
MsG0680034063.01.T01 MTR_8g098925 39.362 94 57 0 8 101 69 162 4.54e-17 73.6
MsG0680034063.01.T01 MTR_4g008270 43.750 80 45 0 5 84 11 90 7.06e-17 71.2
MsG0680034063.01.T01 MTR_2g059470 40.244 82 49 0 6 87 2 83 1.97e-16 69.7
MsG0680034063.01.T01 MTR_3g116210 38.824 85 52 0 4 88 97 181 4.29e-16 71.6
MsG0680034063.01.T01 MTR_7g013520 32.857 140 65 3 4 141 70 182 5.39e-16 71.2
MsG0680034063.01.T01 MTR_7g006380 37.705 122 48 2 4 125 66 159 6.33e-16 71.2
MsG0680034063.01.T01 MTR_3g090920 35.833 120 61 2 23 142 6 109 9.43e-16 68.9
MsG0680034063.01.T01 MTR_6g016265 38.202 89 55 0 2 90 37 125 9.98e-16 69.3
MsG0680034063.01.T01 MTR_2g055490 33.846 130 53 1 12 141 15 111 2.00e-15 68.2
MsG0680034063.01.T01 MTR_1g103580 46.667 75 40 0 67 141 171 245 3.19e-15 70.9
MsG0680034063.01.T01 MTR_4g036855 35.593 118 48 1 24 141 1 90 5.27e-15 66.2
MsG0680034063.01.T01 MTR_5g083200 35.354 99 64 0 2 100 96 194 1.17e-14 68.2
MsG0680034063.01.T01 MTR_0002s1220 35.354 99 64 0 2 100 96 194 1.17e-14 68.2
MsG0680034063.01.T01 MTR_8g031800 42.308 78 45 0 2 79 29 106 1.25e-14 65.9
MsG0680034063.01.T01 MTR_3g111390 35.354 99 64 0 2 100 96 194 1.29e-14 67.8
MsG0680034063.01.T01 MTR_1g070820 35.354 99 64 0 2 100 96 194 1.29e-14 67.8
MsG0680034063.01.T01 MTR_4g098670 36.607 112 52 2 26 137 2 94 1.49e-14 65.5
MsG0680034063.01.T01 MTR_3g010950 33.333 138 62 2 4 141 26 133 2.27e-14 65.9
MsG0680034063.01.T01 MTR_0015s0120 47.887 71 36 1 4 73 293 363 3.32e-14 68.6
MsG0680034063.01.T01 MTR_1g069475 39.080 87 53 0 4 90 164 250 5.87e-14 67.0
MsG0680034063.01.T01 MTR_1g045340 34.343 99 65 0 2 100 82 180 6.69e-14 65.9
MsG0680034063.01.T01 MTR_4g064590 34.211 114 69 2 30 141 52 161 7.38e-14 65.1
MsG0680034063.01.T01 MTR_6g089560 26.241 141 82 1 1 141 113 231 1.24e-13 65.9
MsG0680034063.01.T01 MTR_5g083510 48.438 64 33 0 78 141 28 91 2.52e-13 62.0
MsG0680034063.01.T01 MTR_0093s0070 35.714 84 54 0 3 86 122 205 2.88e-13 64.3
MsG0680034063.01.T01 MTR_2g088140 43.939 66 37 0 65 130 49 114 3.15e-13 62.8
MsG0680034063.01.T01 MTR_8g028785 44.000 75 42 0 33 107 287 361 3.62e-13 65.9
MsG0680034063.01.T01 MTR_5g049680 43.750 64 36 0 4 67 31 94 6.60e-13 61.2
MsG0680034063.01.T01 MTR_3g017480 43.662 71 40 0 70 140 49 119 1.09e-12 61.2
MsG0680034063.01.T01 MTR_8g036690 40.506 79 31 1 35 113 2 64 2.24e-12 58.9
MsG0680034063.01.T01 MTR_5g031620 44.444 81 45 0 61 141 141 221 2.46e-12 62.4
MsG0680034063.01.T01 MTR_6g016355 41.935 62 36 0 4 65 116 177 2.84e-12 61.2
MsG0680034063.01.T01 MTR_8g059135 34.783 92 60 0 7 98 31 122 3.42e-12 60.1
MsG0680034063.01.T01 MTR_8g079540 42.667 75 43 0 3 77 47 121 3.67e-12 59.7
MsG0680034063.01.T01 MTR_5g085210 33.636 110 57 1 4 113 119 212 1.60e-11 60.1
MsG0680034063.01.T01 MTR_1g057740 41.538 65 38 0 76 140 75 139 1.80e-11 58.5
MsG0680034063.01.T01 MTR_1g052310 41.250 80 47 0 60 139 42 121 1.82e-11 58.2
MsG0680034063.01.T01 MTR_1g062840 36.486 74 47 0 26 99 2 75 3.05e-11 56.6
MsG0680034063.01.T01 MTR_1g074090 40.000 65 39 0 3 67 80 144 3.46e-11 57.8
MsG0680034063.01.T01 MTR_1g017840 33.333 81 54 0 4 84 78 158 4.24e-11 57.8
MsG0680034063.01.T01 MTR_5g036320 41.791 67 39 0 4 70 79 145 4.46e-11 57.4
MsG0680034063.01.T01 MTR_3g071490 50.000 54 27 0 88 141 6 59 5.36e-11 55.1
MsG0680034063.01.T01 MTR_1g074240 43.103 58 33 0 6 63 2 59 6.61e-11 55.5
MsG0680034063.01.T01 MTR_5g065870 46.032 63 29 1 79 141 103 160 7.48e-11 57.4
MsG0680034063.01.T01 MTR_7g053400 47.541 61 32 0 5 65 176 236 7.50e-11 58.5
MsG0680034063.01.T01 MTR_5g034450 38.462 65 40 0 4 68 49 113 8.00e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGAAATGAGCTTAATTT+TGG 0.095906 6:-79859424 MsG0680034063.01.T01:CDS
AATAGTAGCAACACTGATTT+TGG 0.129420 6:-79859621 MsG0680034063.01.T01:CDS
TTTCTCGATGTCACATAATA+AGG 0.229395 6:-79862039 None:intergenic
GTGGTACATCAATAAATAAT+TGG 0.231113 6:+79859469 None:intergenic
TGATTTCGTTAATAGTAATA+AGG 0.301928 6:-79861835 MsG0680034063.01.T01:intron
TTAGCCGGAGGCAAGCAAAT+TGG 0.340568 6:-79859536 MsG0680034063.01.T01:CDS
AACGAGCACATATCCTCTAT+TGG 0.345342 6:+79861989 None:intergenic
TTGCAGTGGGTGGCTAAGTT+AGG 0.350857 6:-79861900 MsG0680034063.01.T01:CDS
GTAGATGTAGGCGAAGCTTT+AGG 0.363683 6:-79861936 MsG0680034063.01.T01:CDS
TACTCAATTTACCAACTCTA+AGG 0.364182 6:-79859565 MsG0680034063.01.T01:CDS
TAGCCGGAGGCAAGCAAATT+GGG 0.391931 6:-79859535 MsG0680034063.01.T01:CDS
TGGTACATCAATAAATAATT+GGG 0.425459 6:+79859470 None:intergenic
TAAGTTAGGATTCAAAAGTA+TGG 0.432959 6:-79861886 MsG0680034063.01.T01:CDS
CCGCCCAATTTGCTTGCCTC+CGG 0.469207 6:+79859532 None:intergenic
TACATTCGAGATGCCAATAG+AGG 0.491692 6:-79862002 MsG0680034063.01.T01:CDS
CTGTTATGTTCGGTAGATGT+AGG 0.502651 6:-79861948 MsG0680034063.01.T01:CDS
TTCTGAAGCGTTGCAGTGGG+TGG 0.523656 6:-79861910 MsG0680034063.01.T01:CDS
ATGGTTTGCACTGTTATGTT+CGG 0.533547 6:-79861958 MsG0680034063.01.T01:CDS
TCGATGTCACATAATAAGGT+TGG 0.545966 6:-79862035 MsG0680034063.01.T01:CDS
ACTCTAAGGTAGAGTTTAGC+CGG 0.562364 6:-79859551 MsG0680034063.01.T01:CDS
GCTAAACTCTACCTTAGAGT+TGG 0.582308 6:+79859554 None:intergenic
TTGGGAGCTAGCTTCTAATG+AGG 0.599303 6:+79859488 None:intergenic
ATGTGCTCGTTAAAATGACA+TGG 0.610753 6:-79861977 MsG0680034063.01.T01:CDS
TACTAAGCAGCTGTTGACAA+TGG 0.624558 6:+79859586 None:intergenic
CCGGAGGCAAGCAAATTGGG+CGG 0.636115 6:-79859532 MsG0680034063.01.T01:CDS
AATAAGTCGTTGATACAATG+TGG 0.677541 6:+79859450 None:intergenic
CTAAGGTAGAGTTTAGCCGG+AGG 0.702418 6:-79859548 MsG0680034063.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAAAAATAAAATTTAC+TGG - Chr6:79860759-79860778 MsG0680034063.01.T01:intron 10.0%
!! GACATAATTTAAAAAAAAAA+AGG + Chr6:79861609-79861628 None:intergenic 10.0%
!! AAAAAAAAAAAGGAAAGAAA+AGG + Chr6:79861599-79861618 None:intergenic 15.0%
!! AAAAACCAACATTAAAAAAA+AGG - Chr6:79859812-79859831 MsG0680034063.01.T01:intron 15.0%
!! TGAATGATATAGTAATATTA+TGG - Chr6:79861527-79861546 MsG0680034063.01.T01:intron 15.0%
!!! TATGTATTTTGTTTGATAAA+TGG + Chr6:79859959-79859978 None:intergenic 15.0%
!! AATTAGTGGTTTATTAATTC+AGG - Chr6:79860514-79860533 MsG0680034063.01.T01:intron 20.0%
!! AGCAAAAAAAAAATCATGTT+TGG - Chr6:79860148-79860167 MsG0680034063.01.T01:intron 20.0%
!! CATTAAAAAAAAGGAAAGAA+AGG - Chr6:79859821-79859840 MsG0680034063.01.T01:intron 20.0%
!! TCTTGTTAATTTATTGTTGT+TGG - Chr6:79861474-79861493 MsG0680034063.01.T01:intron 20.0%
!! TGATTTCGTTAATAGTAATA+AGG - Chr6:79859616-79859635 MsG0680034063.01.T01:CDS 20.0%
!! TGGTACATCAATAAATAATT+GGG + Chr6:79861984-79862003 None:intergenic 20.0%
!! TTAATAACTGTTATTATGAC+TGG + Chr6:79860817-79860836 None:intergenic 20.0%
!!! AAAATTGATGTTTTGCAAAA+GGG - Chr6:79861706-79861725 MsG0680034063.01.T01:intron 20.0%
!!! AAAATTGATGTTTTGCAAAA+GGG + Chr6:79861725-79861706 None:intergenic 20.0%
!!! AAACATGATTTTTTTTTTGC+TGG + Chr6:79860149-79860168 None:intergenic 20.0%
!!! AACATGATTTTTTTTTTGCT+GGG + Chr6:79860148-79860167 None:intergenic 20.0%
!!! AATGAAATGAGCTTAATTTT+GGG - Chr6:79862028-79862047 MsG0680034063.01.T01:CDS 20.0%
!!! ACTTTTGCCATATTTTTAAA+AGG - Chr6:79861747-79861766 MsG0680034063.01.T01:intron 20.0%
!!! ATTACATAATTGTGTTTTAC+AGG + Chr6:79861376-79861395 None:intergenic 20.0%
!!! ATTTATTGTTGTTGGATTTT+TGG - Chr6:79861482-79861501 MsG0680034063.01.T01:intron 20.0%
!!! ATTTTATTTTTTGTTTCGTG+AGG + Chr6:79860755-79860774 None:intergenic 20.0%
!!! ATTTTTCGAAAAACAATTTG+TGG + Chr6:79860085-79860104 None:intergenic 20.0%
!!! ATTTTTGGTTTGTTAATTCT+GGG - Chr6:79860574-79860593 MsG0680034063.01.T01:intron 20.0%
!!! CTTTTGCCATATTTTTAAAA+GGG - Chr6:79861748-79861767 MsG0680034063.01.T01:intron 20.0%
!!! TAAAATTGATGTTTTGCAAA+AGG - Chr6:79861705-79861724 MsG0680034063.01.T01:intron 20.0%
!!! TAATGAAATGAGCTTAATTT+TGG - Chr6:79862027-79862046 MsG0680034063.01.T01:CDS 20.0%
!!! TATGGATTTTCTTTAAGAAA+TGG - Chr6:79861545-79861564 MsG0680034063.01.T01:intron 20.0%
!!! TCTTTCCTTTTTTTTAATGT+TGG + Chr6:79859820-79859839 None:intergenic 20.0%
!!! TTATGTTTAGTTTGAATTAG+TGG - Chr6:79860500-79860519 MsG0680034063.01.T01:intron 20.0%
! ACATAATATTATGTTGTTGC+TGG - Chr6:79860459-79860478 MsG0680034063.01.T01:intron 25.0%
! GCTAAATATTTACAAACTAC+AGG - Chr6:79860708-79860727 MsG0680034063.01.T01:intron 25.0%
! GTGGTACATCAATAAATAAT+TGG + Chr6:79861985-79862004 None:intergenic 25.0%
! TAACCTTAACCTCAAAAATT+TGG + Chr6:79860381-79860400 None:intergenic 25.0%
! TAAGAAATGGATGAAGAAAA+TGG - Chr6:79861558-79861577 MsG0680034063.01.T01:intron 25.0%
! TAAGTTAGGATTCAAAAGTA+TGG - Chr6:79859565-79859584 MsG0680034063.01.T01:CDS 25.0%
!! AAATTGATGTTTTGCAAAAG+GGG - Chr6:79861707-79861726 MsG0680034063.01.T01:intron 25.0%
!! AAATTGATGTTTTGCAAAAG+GGG + Chr6:79861726-79861707 None:intergenic 25.0%
!! ACAAACATATTTTCCAAATG+AGG + Chr6:79860911-79860930 None:intergenic 25.0%
!! ATGAAATGAGCTTAATTTTG+GGG - Chr6:79862029-79862048 MsG0680034063.01.T01:CDS 25.0%
!! CAAAATTGATGTTTTGCAAA+AGG + Chr6:79859730-79859749 None:intergenic 25.0%
!! TATTTTATGATCACTGGTAT+CGG + Chr6:79861049-79861068 None:intergenic 25.0%
!! TTCTTGAATCCAAATTTTTG+AGG - Chr6:79860369-79860388 MsG0680034063.01.T01:intron 25.0%
!!! AATCCAAATTTTTGAGGTTA+AGG - Chr6:79860375-79860394 MsG0680034063.01.T01:intron 25.0%
!!! ACTTTTGCCATTTTTTCTAA+AGG + Chr6:79859691-79859710 None:intergenic 25.0%
!!! AGTCTTATTTTACAGGTATA+TGG + Chr6:79860946-79860965 None:intergenic 25.0%
!!! CTCTATTATTTTATGATCAC+TGG + Chr6:79861055-79861074 None:intergenic 25.0%
!!! GATTTTTGGTTTGTTAATTC+GGG - Chr6:79860573-79860592 MsG0680034063.01.T01:intron 25.0%
!!! GATTTTTGGTTTGTTAATTC+TGG - Chr6:79860573-79860592 MsG0680034063.01.T01:intron 25.0%
!!! GCAAAACATCAATTTTAACT+TGG + Chr6:79861702-79861721 None:intergenic 25.0%
!!! GGATTTTTGGTTTGTTAATT+CGG - Chr6:79861495-79861514 MsG0680034063.01.T01:intron 25.0%
!!! GTATTTTGTTTGATAAATGG+TGG + Chr6:79859956-79859975 None:intergenic 25.0%
!!! TAATTCAGGTTTGTTAATTC+TGG - Chr6:79860528-79860547 MsG0680034063.01.T01:intron 25.0%
!!! TACAAAAGAGAGTACTTTTA+TGG + Chr6:79860113-79860132 None:intergenic 25.0%
!!! TTAGTGTTTAGCTTGATTTT+TGG - Chr6:79860559-79860578 MsG0680034063.01.T01:intron 25.0%
!!! TTTTGGACCTTTAGAAAAAA+TGG - Chr6:79859681-79859700 MsG0680034063.01.T01:intron 25.0%
!!! TTTTTTTGCACAAGCTAAAA+TGG - Chr6:79860627-79860646 MsG0680034063.01.T01:intron 25.0%
AAAAAAAGGAAAGAAAGGAG+AGG - Chr6:79859826-79859845 MsG0680034063.01.T01:intron 30.0%
AAATTATGTCAATACATGCG+TGG - Chr6:79861619-79861638 MsG0680034063.01.T01:intron 30.0%
AATAACTCATTGTGTGTGAT+TGG + Chr6:79861126-79861145 None:intergenic 30.0%
AATAAGTCGTTGATACAATG+TGG + Chr6:79862004-79862023 None:intergenic 30.0%
AGGTTAATGCTACTGATAAA+TGG + Chr6:79861356-79861375 None:intergenic 30.0%
ATAACTGTTATTATGACTGG+TGG + Chr6:79860814-79860833 None:intergenic 30.0%
GTTACGAAGAAATATACTTC+AGG + Chr6:79861151-79861170 None:intergenic 30.0%
TAAAATAAGACTTTGTGCAC+CGG - Chr6:79860954-79860973 MsG0680034063.01.T01:intron 30.0%
TACTCAATTTACCAACTCTA+AGG - Chr6:79861886-79861905 MsG0680034063.01.T01:CDS 30.0%
TAGAAAAAGAACGTACACAA+GGG - Chr6:79860325-79860344 MsG0680034063.01.T01:intron 30.0%
TTCTGATTCTACAATGCAAA+GGG + Chr6:79860404-79860423 None:intergenic 30.0%
TTTCGAAAAACAATTTGTGG+TGG + Chr6:79860082-79860101 None:intergenic 30.0%
TTTCTGATTCTACAATGCAA+AGG + Chr6:79860405-79860424 None:intergenic 30.0%
! GTGTTGCTACTATTATTACT+AGG + Chr6:79861824-79861843 None:intergenic 30.0%
! GTTTGCAAAAGTAGAGATTT+TGG + Chr6:79861776-79861795 None:intergenic 30.0%
! TCAAGAAAATTGATTCTCCA+TGG + Chr6:79860356-79860375 None:intergenic 30.0%
! TTTTGCAAAAGGGGTAAATA+TGG + Chr6:79859719-79859738 None:intergenic 30.0%
!! ATCTCTACTTTTGCAAACTT+AGG - Chr6:79861778-79861797 MsG0680034063.01.T01:intron 30.0%
!! CTACCATCAATTTTGCTATA+TGG + Chr6:79860990-79861009 None:intergenic 30.0%
!! TCTCTACTTTTGCAAACTTA+GGG - Chr6:79861779-79861798 MsG0680034063.01.T01:intron 30.0%
!! TTGGCTCCCTTTTAAAAATA+TGG + Chr6:79861757-79861776 None:intergenic 30.0%
!!! AAAAGAGAGTACTTTTATGG+TGG + Chr6:79860110-79860129 None:intergenic 30.0%
!!! AATAGTAGCAACACTGATTT+TGG - Chr6:79861830-79861849 MsG0680034063.01.T01:intron 30.0%
!!! GGACATCTATTTTTATCATG+TGG + Chr6:79861081-79861100 None:intergenic 30.0%
!!! TGCACAAAGTCTTATTTTAC+AGG + Chr6:79860953-79860972 None:intergenic 30.0%
!!! TTTTTTTGCTGGGTTTTGTA+GGG + Chr6:79860138-79860157 None:intergenic 30.0%
!!! TTTTTTTTGCTGGGTTTTGT+AGG + Chr6:79860139-79860158 None:intergenic 30.0%
AAAATAAGACTTTGTGCACC+GGG - Chr6:79860955-79860974 MsG0680034063.01.T01:intron 35.0%
AAGGAAGAGGAAGAAAGAAT+GGG + Chr6:79859881-79859900 None:intergenic 35.0%
ACAAGAGTAGTTTCCTCATT+TGG - Chr6:79860895-79860914 MsG0680034063.01.T01:intron 35.0%
ATAAACAAAGACACCTTGGA+CGG - Chr6:79861213-79861232 MsG0680034063.01.T01:intron 35.0%
ATGCTACTGATAAATGGAGT+TGG + Chr6:79861350-79861369 None:intergenic 35.0%
ATGTGCTCGTTAAAATGACA+TGG - Chr6:79859474-79859493 MsG0680034063.01.T01:CDS 35.0%
ATTATAGGGCAAATGATCCA+AGG - Chr6:79860224-79860243 MsG0680034063.01.T01:intron 35.0%
CGTAACAAATTGCCTCTAAT+CGG - Chr6:79861165-79861184 MsG0680034063.01.T01:intron 35.0%
GTAGAAAAAGAACGTACACA+AGG - Chr6:79860324-79860343 MsG0680034063.01.T01:intron 35.0%
TCAATCCTATGTATCATGAC+AGG + Chr6:79861679-79861698 None:intergenic 35.0%
TGTGATATTATCGAAACAGC+AGG + Chr6:79861102-79861121 None:intergenic 35.0%
TTATAGGGCAAATGATCCAA+GGG - Chr6:79860225-79860244 MsG0680034063.01.T01:intron 35.0%
TTATGTTGTTGCTGGATTTC+TGG - Chr6:79860467-79860486 MsG0680034063.01.T01:intron 35.0%
TTGCTTTCATCCACAAAAAG+AGG - Chr6:79860849-79860868 MsG0680034063.01.T01:intron 35.0%
! ATCAAATGCACCTCTTTTTG+TGG + Chr6:79860862-79860881 None:intergenic 35.0%
! ATGGTTTGCACTGTTATGTT+CGG - Chr6:79859493-79859512 MsG0680034063.01.T01:CDS 35.0%
! GTTTGCAAAAGTAGCGATTT+TGG - Chr6:79859664-79859683 MsG0680034063.01.T01:intron 35.0%
! TCGCTTTATTGTTAGTCATG+AGG + Chr6:79861286-79861305 None:intergenic 35.0%
!! CATCAATTTTGCTATATGGC+GGG + Chr6:79860986-79861005 None:intergenic 35.0%
!! CCATCAATTTTGCTATATGG+CGG + Chr6:79860987-79861006 None:intergenic 35.0%
!! CTCTACTTTTGCAAACTTAG+GGG - Chr6:79861780-79861799 MsG0680034063.01.T01:intron 35.0%
!! TCTACTTTTGCAAACTTAGG+GGG - Chr6:79861781-79861800 MsG0680034063.01.T01:intron 35.0%
!!! CGCTACTTTTGCAAACTTAA+GGG + Chr6:79859660-79859679 None:intergenic 35.0%
!!! GCTACTTTTGCAAACTTAAG+GGG + Chr6:79859659-79859678 None:intergenic 35.0%
!!! TCGCTACTTTTGCAAACTTA+AGG + Chr6:79859661-79859680 None:intergenic 35.0%
AAATAAGACTTTGTGCACCG+GGG - Chr6:79860956-79860975 MsG0680034063.01.T01:intron 40.0%
AACGAGCACATATCCTCTAT+TGG + Chr6:79859465-79859484 None:intergenic 40.0%
AATTGCCTCTAATCGGAAGT+CGG - Chr6:79861172-79861191 MsG0680034063.01.T01:intron 40.0%
ACACACCTGTCATGATACAT+AGG - Chr6:79861671-79861690 MsG0680034063.01.T01:intron 40.0%
ACAGGATGCCAAACAAAGAA+AGG - Chr6:79860726-79860745 MsG0680034063.01.T01:intron 40.0%
ACTGGTACACTCTCATACAA+AGG - Chr6:79860777-79860796 MsG0680034063.01.T01:intron 40.0%
AGGAAGAGGAAGAAAGAATG+GGG + Chr6:79859880-79859899 None:intergenic 40.0%
ATCCAAGTGAACTCCATTTC+TGG - Chr6:79860283-79860302 MsG0680034063.01.T01:intron 40.0%
CCACATAAACAAAGACACCT+TGG - Chr6:79861209-79861228 MsG0680034063.01.T01:intron 40.0%
CCGCCATATAGCAAAATTGA+TGG - Chr6:79860984-79861003 MsG0680034063.01.T01:intron 40.0%
CCTTAGTAACATCCCAGAAA+TGG + Chr6:79860299-79860318 None:intergenic 40.0%
CTGTTATGTTCGGTAGATGT+AGG - Chr6:79859503-79859522 MsG0680034063.01.T01:CDS 40.0%
GAAGGAAGAGGAAGAAAGAA+TGG + Chr6:79859882-79859901 None:intergenic 40.0%
GCTAAACTCTACCTTAGAGT+TGG + Chr6:79861900-79861919 None:intergenic 40.0%
GGACTCTTGACTTTGTTCAT+GGG + Chr6:79861329-79861348 None:intergenic 40.0%
GTCATGAGGATTACTCAGAT+AGG + Chr6:79861272-79861291 None:intergenic 40.0%
TACACTCTCATACAAAGGAG+CGG - Chr6:79860782-79860801 MsG0680034063.01.T01:intron 40.0%
TACATTCGAGATGCCAATAG+AGG - Chr6:79859449-79859468 MsG0680034063.01.T01:CDS 40.0%
TACTAAGCAGCTGTTGACAA+TGG + Chr6:79861868-79861887 None:intergenic 40.0%
TCAAGATCTGACAATAGAGG+TGG + Chr6:79860059-79860078 None:intergenic 40.0%
TCCAAGTGAACTCCATTTCT+GGG - Chr6:79860284-79860303 MsG0680034063.01.T01:intron 40.0%
TGAGGATGCCTTTCTTTGTT+TGG + Chr6:79860737-79860756 None:intergenic 40.0%
TGCAAAAGGGGTAAATATGG+AGG + Chr6:79859716-79859735 None:intergenic 40.0%
TGGACTCTTGACTTTGTTCA+TGG + Chr6:79861330-79861349 None:intergenic 40.0%
TGGTCAAGATCTGACAATAG+AGG + Chr6:79860062-79860081 None:intergenic 40.0%
TTGCCCTATAATTTCCGACA+CGG + Chr6:79860216-79860235 None:intergenic 40.0%
! AATCCAGCAACTTGTTTGCT+TGG + Chr6:79861431-79861450 None:intergenic 40.0%
! CCAAGGTGTCTTTGTTTATG+TGG + Chr6:79861212-79861231 None:intergenic 40.0%
! CTTTTTGCACGTCTTGTGTA+GGG + Chr6:79860679-79860698 None:intergenic 40.0%
!! ACTCTAAGGTAGAGTTTAGC+CGG - Chr6:79861900-79861919 MsG0680034063.01.T01:CDS 40.0%
!! CCATTTCTGGGATGTTACTA+AGG - Chr6:79860296-79860315 MsG0680034063.01.T01:intron 40.0%
!!! ATTTTCTGAAGCGTTGCAGT+GGG - Chr6:79859538-79859557 MsG0680034063.01.T01:CDS 40.0%
AAGAGGAAGAAAGAATGGGG+AGG + Chr6:79859877-79859896 None:intergenic 45.0%
ACACAGCTGACATGACACAT+AGG + Chr6:79859764-79859783 None:intergenic 45.0%
ACACTCTCATACAAAGGAGC+GGG - Chr6:79860783-79860802 MsG0680034063.01.T01:intron 45.0%
ACCACCGTGTCGGAAATTAT+AGG - Chr6:79860209-79860228 MsG0680034063.01.T01:intron 45.0%
ATGTTTGGATGACCTTGCTC+CGG - Chr6:79860163-79860182 MsG0680034063.01.T01:intron 45.0%
CCACCGTGTCGGAAATTATA+GGG - Chr6:79860210-79860229 MsG0680034063.01.T01:intron 45.0%
CTTCCAAGCAAACAAGTTGC+TGG - Chr6:79861425-79861444 MsG0680034063.01.T01:intron 45.0%
GTAGATGTAGGCGAAGCTTT+AGG - Chr6:79859515-79859534 MsG0680034063.01.T01:CDS 45.0%
TCCCAGAAATGGAGTTCACT+TGG + Chr6:79860288-79860307 None:intergenic 45.0%
TTGTTCATGGGTATTCCGTG+CGG + Chr6:79861317-79861336 None:intergenic 45.0%
! CAACTTGTTTGCTTGGAAGG+AGG + Chr6:79861424-79861443 None:intergenic 45.0%
! CAGCAACTTGTTTGCTTGGA+AGG + Chr6:79861427-79861446 None:intergenic 45.0%
! GCTTTTTGCACGTCTTGTGT+AGG + Chr6:79860680-79860699 None:intergenic 45.0%
! GGAGTCTTGTCGACAATGTT+TGG + Chr6:79861251-79861270 None:intergenic 45.0%
! TGGGTTAGGAAAGAAGGAAG+AGG + Chr6:79859894-79859913 None:intergenic 45.0%
! TTCTGGGTTTGTGTTGTTGC+TGG - Chr6:79860590-79860609 MsG0680034063.01.T01:intron 45.0%
! TTGGGAGCTAGCTTCTAATG+AGG + Chr6:79861966-79861985 None:intergenic 45.0%
!!! GATTTTCTGAAGCGTTGCAG+TGG - Chr6:79859537-79859556 MsG0680034063.01.T01:CDS 45.0%
AAAGCGATAAGCTTCCCGCA+CGG - Chr6:79861299-79861318 MsG0680034063.01.T01:intron 50.0%
ATCCAAGGGAGTGAAGCCAA+TGG - Chr6:79860239-79860258 MsG0680034063.01.T01:intron 50.0%
CCCTATAATTTCCGACACGG+TGG + Chr6:79860213-79860232 None:intergenic 50.0%
CTAAGGTAGAGTTTAGCCGG+AGG - Chr6:79861903-79861922 MsG0680034063.01.T01:CDS 50.0%
TAGCCGGAGGCAAGCAAATT+GGG - Chr6:79861916-79861935 MsG0680034063.01.T01:CDS 50.0%
TGTTCATGGGTATTCCGTGC+GGG + Chr6:79861316-79861335 None:intergenic 50.0%
TTAGCCGGAGGCAAGCAAAT+TGG - Chr6:79861915-79861934 MsG0680034063.01.T01:CDS 50.0%
TTGCAGTGGGTGGCTAAGTT+AGG - Chr6:79859551-79859570 MsG0680034063.01.T01:CDS 50.0%
! CACTGGTATCGGATTGTCTC+TGG + Chr6:79861038-79861057 None:intergenic 50.0%
!! AATGGTGGTCAGAAGCACCA+CGG + Chr6:79859941-79859960 None:intergenic 50.0%
ACGACGTCAACCTTCGCCAT+TGG + Chr6:79860258-79860277 None:intergenic 55.0%
GCATACGCACATACCGTCCA+AGG + Chr6:79861229-79861248 None:intergenic 55.0%
GGGAAACATCACCACCGTGT+CGG - Chr6:79860199-79860218 MsG0680034063.01.T01:intron 55.0%
TCGGAAGTCGGCTGCGTAAA+AGG - Chr6:79861184-79861203 MsG0680034063.01.T01:intron 55.0%
! GACACGGTGGTGATGTTTCC+CGG + Chr6:79860200-79860219 None:intergenic 55.0%
!! CGTACACAAGGGTATCGCCA+TGG - Chr6:79860336-79860355 MsG0680034063.01.T01:intron 55.0%
!! TTCTGAAGCGTTGCAGTGGG+TGG - Chr6:79859541-79859560 MsG0680034063.01.T01:CDS 55.0%
ACGGTGGGTGGCCGGAAAAA+GGG + Chr6:79859922-79859941 None:intergenic 60.0%
CCGGAGGCAAGCAAATTGGG+CGG - Chr6:79861919-79861938 MsG0680034063.01.T01:CDS 60.0%
GGGAGTGAAGCCAATGGCGA+AGG - Chr6:79860245-79860264 MsG0680034063.01.T01:intron 60.0%
GGTGATGTTTCCCGGTGTGG+TGG + Chr6:79860192-79860211 None:intergenic 60.0%
! AACCCACCGCCCCCTTTTTC+CGG - Chr6:79859908-79859927 MsG0680034063.01.T01:intron 60.0%
! CCGCCCAATTTGCTTGCCTC+CGG + Chr6:79861922-79861941 None:intergenic 60.0%
! CGCAGCCGACTTCCGATTAG+AGG + Chr6:79861180-79861199 None:intergenic 60.0%
! GAAAAAGGGGGCGGTGGGTT+AGG + Chr6:79859908-79859927 None:intergenic 60.0%
! GCTATATGGCGGGACTACCC+CGG + Chr6:79860976-79860995 None:intergenic 60.0%
! GGTGGTGATGTTTCCCGGTG+TGG + Chr6:79860195-79860214 None:intergenic 60.0%
!! CGCCATTGGCTTCACTCCCT+TGG + Chr6:79860244-79860263 None:intergenic 60.0%
!! GGGCGGTGGGTTAGGAAAGA+AGG + Chr6:79859900-79859919 None:intergenic 60.0%
!! GTGGTCAGAAGCACCACGGT+GGG + Chr6:79859937-79859956 None:intergenic 60.0%
CACGGTGGGTGGCCGGAAAA+AGG + Chr6:79859923-79859942 None:intergenic 65.0%
CGGTGGGTGGCCGGAAAAAG+GGG + Chr6:79859921-79859940 None:intergenic 65.0%
GGTGGGTGGCCGGAAAAAGG+GGG + Chr6:79859920-79859939 None:intergenic 65.0%
TTTCCCGGTGTGGTGGTCGC+CGG + Chr6:79860185-79860204 None:intergenic 65.0%
! CTTTTTCCGGCCACCCACCG+TGG - Chr6:79859921-79859940 MsG0680034063.01.T01:intron 65.0%
!! GGTGGTCAGAAGCACCACGG+TGG + Chr6:79859938-79859957 None:intergenic 65.0%
!! GTCAGAAGCACCACGGTGGG+TGG + Chr6:79859934-79859953 None:intergenic 65.0%
GGCCGGAAAAAGGGGGCGGT+GGG + Chr6:79859913-79859932 None:intergenic 70.0%
GGGTGGCCGGAAAAAGGGGG+CGG + Chr6:79859917-79859936 None:intergenic 70.0%
GTGTGGTGGTCGCCGGAGCA+AGG + Chr6:79860178-79860197 None:intergenic 70.0%
TGCTCCGGCGACCACCACAC+CGG - Chr6:79860178-79860197 MsG0680034063.01.T01:intron 70.0%
TGGCCGGAAAAAGGGGGCGG+TGG + Chr6:79859914-79859933 None:intergenic 70.0%
! GAAGCACCACGGTGGGTGGC+CGG + Chr6:79859930-79859949 None:intergenic 70.0%
GCTCCGGCGACCACCACACC+GGG - Chr6:79860179-79860198 MsG0680034063.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 79859419 79862054 79859419 ID=MsG0680034063.01;Name=MsG0680034063.01
Chr6 mRNA 79859419 79862054 79859419 ID=MsG0680034063.01.T01;Parent=MsG0680034063.01;Name=MsG0680034063.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|146
Chr6 exon 79861844 79862054 79861844 ID=MsG0680034063.01.T01:exon:9738;Parent=MsG0680034063.01.T01
Chr6 exon 79859419 79859648 79859419 ID=MsG0680034063.01.T01:exon:9739;Parent=MsG0680034063.01.T01
Chr6 CDS 79861844 79862054 79861844 ID=MsG0680034063.01.T01:cds;Parent=MsG0680034063.01.T01
Chr6 CDS 79859419 79859648 79859419 ID=MsG0680034063.01.T01:cds;Parent=MsG0680034063.01.T01
Chr6 mRNA 79861830 79862054 79861830 ID=MsG0680034063.01.T02;Parent=MsG0680034063.01;Name=MsG0680034063.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|74
Chr6 exon 79861830 79862054 79861830 ID=MsG0680034063.01.T02:exon:9740;Parent=MsG0680034063.01.T02
Chr6 CDS 79861830 79862054 79861830 ID=MsG0680034063.01.T02:cds;Parent=MsG0680034063.01.T02
Gene Sequence

>MsG0680034063.01.T02

ATGTCACATAATAAGGTTGGTGTCAGTATGTACATTCGAGATGCCAATAGAGGATATGTGCTCGTTAAAATGACATGGTTTGCACTGTTATGTTCGGTAGATGTAGGCGAAGCTTTAGGATTTTCTGAAGCGTTGCAGTGGGTGGCTAAGTTAGGATTCAAAAGTATGGACTTCTTATTGAATTTGAAGCTCGTCGTTGATTTCGTTAATAGTAATAAGGTTTAA

>MsG0680034063.01.T01

ATGTCACATAATAAGGTTGGTGTCAGTATGTACATTCGAGATGCCAATAGAGGATATGTGCTCGTTAAAATGACATGGTTTGCACTGTTATGTTCGGTAGATGTAGGCGAAGCTTTAGGATTTTCTGAAGCGTTGCAGTGGGTGGCTAAGTTAGGATTCAAAAGTATGGACTTCTTATTGAATTTGAAGCTCGTCGTTGATTTCGTTAATAGTAATAATAGTAGCAACACTGATTTTGGAAGAATTACTAGCCATTGTCAACAGCTGCTTAGTACTCAATTTACCAACTCTAAGGTAGAGTTTAGCCGGAGGCAAGCAAATTGGGCGGCTCATGAATTAGTGCATGCAACCTCATTAGAAGCTAGCTCCCAATTATTTATTGATGTACCACATTGTATCAACGACTTATTATCTAATGAAATGAGCTTAATTTTGGGGTAA
Protein sequence

>MsG0680034063.01.T02

MSHNKVGVSMYIRDANRGYVLVKMTWFALLCSVDVGEALGFSEALQWVAKLGFKSMDFLLNLKLVVDFVNSNKV*

>MsG0680034063.01.T01

MSHNKVGVSMYIRDANRGYVLVKMTWFALLCSVDVGEALGFSEALQWVAKLGFKSMDFLLNLKLVVDFVNSNNSSNTDFGRITSHCQQLLSTQFTNSKVEFSRRQANWAAHELVHATSLEASSQLFIDVPHCINDLLSNEMSLILG*