Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034166.01.T01 | KEH18345.1 | 68.421 | 76 | 16 | 1 | 1 | 76 | 1 | 68 | 1.89E-27 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034166.01.T01 | A0A072TXU0 | 68.421 | 76 | 16 | 1 | 1 | 76 | 1 | 68 | 9.02e-28 | 104 |
| MsG0680034166.01.T01 | A0A396GCQ8 | 68.421 | 76 | 16 | 1 | 1 | 76 | 68 | 135 | 2.88e-27 | 105 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032844.01 | MsG0680034166.01 | 0.811645 | 6.310573e-51 | 3.927456e-48 |
| MsG0380016475.01 | MsG0680034166.01 | 0.811687 | 6.179239e-51 | 3.850085e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034166.01.T01 | MTR_8g016250 | 68.421 | 76 | 16 | 1 | 1 | 76 | 1 | 68 | 2.29e-31 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACTTGTTTCATCAGTTGA+TGG | 0.266309 | 6:+82440440 | None:intergenic |
| ACCTATAATTAAAAGTTAAA+TGG | 0.277682 | 6:+82440344 | None:intergenic |
| GGAGGCTGCGGCTACTGATA+TGG | 0.303346 | 6:-82440465 | MsG0680034166.01.T01:CDS |
| GTGTTTCTTCCTCTCCCTCT+TGG | 0.387793 | 6:+82440388 | None:intergenic |
| AATAGCATCCACATCTAAAC+GGG | 0.399215 | 6:+82440550 | None:intergenic |
| TAGAGGCTTATGAGAAGAAT+AGG | 0.402718 | 6:-82440514 | MsG0680034166.01.T01:CDS |
| AAATAGCATCCACATCTAAA+CGG | 0.431811 | 6:+82440549 | None:intergenic |
| AGAGGCTTATGAGAAGAATA+GGG | 0.465349 | 6:-82440513 | MsG0680034166.01.T01:CDS |
| TTTCTTCCTCTCCCTCTTGG+TGG | 0.479075 | 6:+82440391 | None:intergenic |
| GAGGCTGCGGCTACTGATAT+GGG | 0.482882 | 6:-82440464 | MsG0680034166.01.T01:CDS |
| ATGAGACCACCAAGAGGGAG+AGG | 0.523170 | 6:-82440397 | MsG0680034166.01.T01:CDS |
| AAAACAAAAGTTGGAGGCTG+CGG | 0.526619 | 6:-82440477 | MsG0680034166.01.T01:CDS |
| TCTCTAAACCGGCAGATGAA+GGG | 0.581647 | 6:-82440575 | None:intergenic |
| GAAGGGTCCCCGTTTAGATG+TGG | 0.593864 | 6:-82440558 | MsG0680034166.01.T01:CDS |
| GAAGAATGAGACCACCAAGA+GGG | 0.610240 | 6:-82440402 | MsG0680034166.01.T01:CDS |
| TATTTGTGCTGCAATGCTAG+AGG | 0.629204 | 6:-82440531 | MsG0680034166.01.T01:CDS |
| AGAAGAATGAGACCACCAAG+AGG | 0.668779 | 6:-82440403 | MsG0680034166.01.T01:CDS |
| CATCAACTGATGAAACAAGT+CGG | 0.684167 | 6:-82440439 | MsG0680034166.01.T01:CDS |
| ATAGCATCCACATCTAAACG+GGG | 0.788620 | 6:+82440551 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGGGAAAAAAAACAAAAGT+TGG | - | Chr6:82440426-82440445 | MsG0680034166.01.T01:CDS | 25.0% |
| AAATAGCATCCACATCTAAA+CGG | + | Chr6:82440366-82440385 | None:intergenic | 30.0% | |
| GGAAAAAAAACAAAAGTTGG+AGG | - | Chr6:82440429-82440448 | MsG0680034166.01.T01:CDS | 30.0% | |
| ! | ATGAGAAGAATAGGGTTTTA+TGG | - | Chr6:82440407-82440426 | MsG0680034166.01.T01:CDS | 30.0% |
| !! | TGAGAAGAATAGGGTTTTAT+GGG | - | Chr6:82440408-82440427 | MsG0680034166.01.T01:CDS | 30.0% |
| AATAGCATCCACATCTAAAC+GGG | + | Chr6:82440365-82440384 | None:intergenic | 35.0% | |
| AGAGGCTTATGAGAAGAATA+GGG | - | Chr6:82440399-82440418 | MsG0680034166.01.T01:CDS | 35.0% | |
| CATCAACTGATGAAACAAGT+CGG | - | Chr6:82440473-82440492 | MsG0680034166.01.T01:CDS | 35.0% | |
| TAGAGGCTTATGAGAAGAAT+AGG | - | Chr6:82440398-82440417 | MsG0680034166.01.T01:CDS | 35.0% | |
| AAAACAAAAGTTGGAGGCTG+CGG | - | Chr6:82440435-82440454 | MsG0680034166.01.T01:CDS | 40.0% | |
| ATAGCATCCACATCTAAACG+GGG | + | Chr6:82440364-82440383 | None:intergenic | 40.0% | |
| TATTTGTGCTGCAATGCTAG+AGG | - | Chr6:82440381-82440400 | MsG0680034166.01.T01:CDS | 40.0% | |
| ! | CGACTTGTTTCATCAGTTGA+TGG | + | Chr6:82440475-82440494 | None:intergenic | 40.0% |
| AGAAGAATGAGACCACCAAG+AGG | - | Chr6:82440509-82440528 | MsG0680034166.01.T01:CDS | 45.0% | |
| GAAGAATGAGACCACCAAGA+GGG | - | Chr6:82440510-82440529 | MsG0680034166.01.T01:CDS | 45.0% | |
| GTGTTTCTTCCTCTCCCTCT+TGG | + | Chr6:82440527-82440546 | None:intergenic | 50.0% | |
| TTTCTTCCTCTCCCTCTTGG+TGG | + | Chr6:82440524-82440543 | None:intergenic | 50.0% | |
| ATGAGACCACCAAGAGGGAG+AGG | - | Chr6:82440515-82440534 | MsG0680034166.01.T01:CDS | 55.0% | |
| GAAGGGTCCCCGTTTAGATG+TGG | - | Chr6:82440354-82440373 | MsG0680034166.01.T01:CDS | 55.0% | |
| GAGGCTGCGGCTACTGATAT+GGG | - | Chr6:82440448-82440467 | MsG0680034166.01.T01:CDS | 55.0% | |
| GGAGGCTGCGGCTACTGATA+TGG | - | Chr6:82440447-82440466 | MsG0680034166.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 82440352 | 82440582 | 82440352 | ID=MsG0680034166.01;Name=MsG0680034166.01 |
| Chr6 | mRNA | 82440352 | 82440582 | 82440352 | ID=MsG0680034166.01.T01;Parent=MsG0680034166.01;Name=MsG0680034166.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|76 |
| Chr6 | exon | 82440352 | 82440582 | 82440352 | ID=MsG0680034166.01.T01:exon:11542;Parent=MsG0680034166.01.T01 |
| Chr6 | CDS | 82440352 | 82440582 | 82440352 | ID=MsG0680034166.01.T01:cds;Parent=MsG0680034166.01.T01 |
| Gene Sequence |
| Protein sequence |
