AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034294.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034294.01.T01 MTR_7g057340 66.279 172 58 0 1 172 1 172 2.93e-87 253
MsG0680034294.01.T01 MTR_4g048460 69.186 172 53 0 1 172 390 561 5.41e-83 255
MsG0680034294.01.T01 MTR_7g057390 63.953 172 59 1 1 172 1 169 2.12e-80 236
MsG0680034294.01.T01 MTR_6g039440 65.116 172 60 0 1 172 382 553 2.79e-78 243
MsG0680034294.01.T01 MTR_3g464190 63.953 172 62 0 1 172 386 557 4.12e-77 240
MsG0680034294.01.T01 MTR_5g073260 63.529 170 62 0 1 170 381 550 1.29e-75 236
MsG0680034294.01.T01 MTR_6g008560 61.047 172 67 0 1 172 230 401 2.40e-75 231
MsG0680034294.01.T01 MTR_5g062230 64.535 172 61 0 1 172 382 553 1.52e-74 233
MsG0680034294.01.T01 MTR_5g094620 62.209 172 65 0 1 172 366 537 3.48e-74 232
MsG0680034294.01.T01 MTR_5g073200 61.176 170 66 0 1 170 378 547 1.04e-73 231
MsG0680034294.01.T01 MTR_6g008560 61.047 172 67 0 1 172 386 557 2.03e-73 230
MsG0680034294.01.T01 MTR_4g081460 55.030 169 76 0 1 169 391 559 5.73e-62 201
MsG0680034294.01.T01 MTR_3g015080 48.256 172 51 2 1 172 1 134 1.74e-50 159
MsG0680034294.01.T01 MTR_2g081980 42.690 171 95 3 3 172 389 557 4.51e-41 145
MsG0680034294.01.T01 MTR_8g007515 38.150 173 104 3 1 172 386 556 2.74e-37 135
MsG0680034294.01.T01 MTR_2g082050 38.012 171 103 3 1 170 289 457 3.87e-37 133
MsG0680034294.01.T01 MTR_2g082010 38.150 173 104 3 1 172 398 568 1.66e-36 132
MsG0680034294.01.T01 MTR_2g082060 37.572 173 94 4 1 172 394 553 8.84e-31 117
MsG0680034294.01.T01 MTR_4g048460 69.333 75 23 0 1 75 390 464 1.66e-29 112
MsG0680034294.01.T01 MTR_4g019550 36.129 155 94 3 19 172 408 558 1.50e-24 99.4
MsG0680034294.01.T01 MTR_4g092020 30.636 173 115 2 1 170 399 569 3.88e-21 89.7
MsG0680034294.01.T01 MTR_7g010710 29.341 167 118 0 3 169 386 552 5.08e-18 80.9
MsG0680034294.01.T01 MTR_3g063120 27.647 170 117 4 1 166 321 488 1.79e-16 76.3
MsG0680034294.01.T01 MTR_6g065040 28.205 156 104 3 1 150 243 396 5.75e-16 74.7
MsG0680034294.01.T01 MTR_4g045810 25.294 170 127 0 1 170 410 579 8.44e-16 74.3
MsG0680034294.01.T01 MTR_3g052120 30.000 170 113 4 1 166 345 512 1.27e-15 73.9
MsG0680034294.01.T01 MTR_4g092010 26.036 169 117 3 1 164 391 556 1.43e-15 73.9
MsG0680034294.01.T01 MTR_3g465090 27.922 154 107 3 1 150 345 498 3.09e-15 72.8
MsG0680034294.01.T01 MTR_3g063140 28.205 156 104 3 1 150 345 498 4.89e-15 72.0
MsG0680034294.01.T01 MTR_2g089130 25.490 153 112 2 1 152 395 546 7.57e-15 71.6
MsG0680034294.01.T01 MTR_3g063170 28.477 151 100 3 1 145 345 493 9.14e-15 71.2
MsG0680034294.01.T01 MTR_6g064980 27.329 161 109 3 1 155 345 503 1.51e-14 70.9
MsG0680034294.01.T01 MTR_2g065450 25.595 168 124 1 3 170 422 588 4.59e-13 66.6
MsG0680034294.01.T01 MTR_2g010960 27.168 173 113 5 1 163 349 518 5.19e-13 66.2
MsG0680034294.01.T01 MTR_2g064425 25.000 168 125 1 3 170 418 584 8.29e-11 60.1
MsG0680034294.01.T01 MTR_2g064425 25.000 168 125 1 3 170 403 569 9.42e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034294.01.T01 AT5G23960 40.698 172 101 1 1 172 377 547 6.07e-42 147
MsG0680034294.01.T01 AT5G23960 40.698 172 101 1 1 172 375 545 6.51e-42 147
MsG0680034294.01.T01 AT4G13300 32.353 170 114 1 4 172 385 554 1.03e-29 114
MsG0680034294.01.T01 AT5G48110 31.361 169 113 3 4 172 174 339 1.78e-29 110
MsG0680034294.01.T01 AT1G70080 34.104 173 113 1 1 172 439 611 2.59e-29 113
MsG0680034294.01.T01 AT4G13280 31.953 169 114 1 5 172 386 554 8.25e-29 111
MsG0680034294.01.T01 AT4G20200 31.953 169 113 2 5 172 437 604 2.33e-28 110
MsG0680034294.01.T01 AT3G32030 30.058 173 120 1 1 172 432 604 5.78e-28 109
MsG0680034294.01.T01 AT3G29410 30.539 167 116 0 4 170 330 496 5.80e-28 108
MsG0680034294.01.T01 AT3G29410 30.178 169 118 0 4 172 435 603 8.44e-28 108
MsG0680034294.01.T01 AT5G44630 31.928 166 113 0 5 170 390 555 2.51e-27 107
MsG0680034294.01.T01 AT1G61680 34.969 163 105 1 1 162 400 562 4.08e-27 106
MsG0680034294.01.T01 AT1G61680 34.969 163 105 1 1 162 407 569 4.32e-27 106
MsG0680034294.01.T01 AT3G14520 29.762 168 117 1 6 172 437 604 2.10e-26 104
MsG0680034294.01.T01 AT1G33750 28.402 169 121 0 4 172 435 603 2.70e-25 101
MsG0680034294.01.T01 AT3G14490 33.529 170 111 2 4 172 433 601 5.26e-25 100
MsG0680034294.01.T01 AT4G20210 27.811 169 122 0 4 172 432 600 1.02e-24 100
MsG0680034294.01.T01 AT1G31950 28.824 170 120 1 4 172 437 606 2.20e-24 99.0
MsG0680034294.01.T01 AT1G31950 28.824 170 120 1 4 172 438 607 2.41e-24 99.0
MsG0680034294.01.T01 AT3G14540 28.571 168 119 1 6 172 434 601 3.70e-24 98.6
MsG0680034294.01.T01 AT1G48800 26.667 165 121 0 4 168 438 602 1.65e-22 94.0
MsG0680034294.01.T01 AT4G13280 34.351 131 85 1 43 172 394 524 9.40e-22 91.7
MsG0680034294.01.T01 AT3G29190 27.647 170 115 2 5 170 434 599 9.65e-22 91.7
MsG0680034294.01.T01 AT4G15870 27.976 168 120 1 6 172 431 598 1.36e-21 91.3
MsG0680034294.01.T01 AT4G20230 28.313 166 118 1 4 168 448 613 1.38e-21 91.3
MsG0680034294.01.T01 AT4G20230 28.313 166 118 1 4 168 436 601 1.41e-21 91.3
MsG0680034294.01.T01 AT2G23230 28.485 165 117 1 4 167 431 595 1.35e-19 85.5
MsG0680034294.01.T01 AT2G23230 28.485 165 117 1 4 167 426 590 1.50e-19 85.1
MsG0680034294.01.T01 AT3G29110 28.743 167 119 0 4 170 429 595 2.99e-19 84.3
MsG0680034294.01.T01 AT1G66020 27.811 169 122 0 4 172 430 598 1.19e-18 82.8
MsG0680034294.01.T01 AT1G48820 25.153 163 121 1 4 165 395 557 9.53e-18 80.1
MsG0680034294.01.T01 AT4G16730 24.855 173 127 2 1 170 319 491 1.27e-13 68.2
MsG0680034294.01.T01 AT4G16730 24.855 173 127 2 1 170 365 537 1.45e-13 68.2
MsG0680034294.01.T01 AT3G25810 25.281 178 127 3 1 172 420 597 1.48e-13 68.2
MsG0680034294.01.T01 AT4G16740 26.590 173 122 4 1 172 396 564 9.64e-11 59.7

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTATACATTGCAGGCTT+AGG 0.276774 6:-85210896 MsG0680034294.01.T01:CDS
AAACAGTTTGAGCAGAAAAT+AGG 0.306839 6:-85210747 MsG0680034294.01.T01:intron
TGAGGGAATATGATTTGAAT+AGG 0.340079 6:-85210695 MsG0680034294.01.T01:CDS
ATGCTGCTGCTATACATTGC+AGG 0.354080 6:-85210902 MsG0680034294.01.T01:CDS
AGGACATGTTTCTTCGTACT+TGG 0.357426 6:-85210727 MsG0680034294.01.T01:CDS
CGTACTTGGAATGTTATATG+AGG 0.388936 6:-85210713 MsG0680034294.01.T01:CDS
AATAAATACTCAAATTTCTA+CGG 0.417455 6:+85210461 None:intergenic
TGCATGTACTCCTCTGTTGT+TGG 0.418606 6:+85211041 None:intergenic
AAAAGAAAATTGACAACGCT+TGG 0.444020 6:-85210650 MsG0680034294.01.T01:CDS
TGCTATACATTGCAGGCTTA+GGG 0.448464 6:-85210895 MsG0680034294.01.T01:CDS
TACGACTTCTTACATTGGAA+TGG 0.454510 6:-85210982 MsG0680034294.01.T01:CDS
ATAGTTAGCATTCAGCCATC+TGG 0.458951 6:+85211067 None:intergenic
ACGACTTCTTACATTGGAAT+GGG 0.460983 6:-85210981 MsG0680034294.01.T01:CDS
GAGGGAATATGATTTGAATA+GGG 0.475500 6:-85210694 MsG0680034294.01.T01:CDS
ATAAATACTCAAATTTCTAC+GGG 0.477038 6:+85210462 None:intergenic
CGCTTGGAAAGATATAAATC+AGG 0.490061 6:-85210634 MsG0680034294.01.T01:CDS
TTGAGAACACACGTAAGAAT+AGG 0.491590 6:+85210582 None:intergenic
CGACAAAAGACATCTTTGAC+TGG 0.519009 6:-85210950 MsG0680034294.01.T01:CDS
TAAGAAGTCGTAGCCAACAC+TGG 0.522936 6:+85210993 None:intergenic
ATAGGTAACGGAACTTCAGT+TGG 0.529746 6:+85210600 None:intergenic
CTTATATGAGTGAGGCCAGA+TGG 0.549142 6:-85211082 None:intergenic
GTACTTGGAATGTTATATGA+GGG 0.567173 6:-85210712 MsG0680034294.01.T01:CDS
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6:-85211006 MsG0680034294.01.T01:CDS
TTGGCTACGACTTCTTACAT+TGG 0.586299 6:-85210987 MsG0680034294.01.T01:CDS
CACATATCAACAATATCAAG+TGG 0.594145 6:-85211020 MsG0680034294.01.T01:CDS
GATAACTACACACACTCTGA+CGG 0.610210 6:-85210522 MsG0680034294.01.T01:CDS
AAGAAGTCGTAGCCAACACT+GGG 0.613680 6:+85210994 None:intergenic
GACAAAAGACATCTTTGACT+GGG 0.626667 6:-85210949 MsG0680034294.01.T01:CDS
AATCCAAGCTTATATGAGTG+AGG 0.644710 6:-85211090 None:intergenic
GGATGAAATTGTGTCAAGCG+AGG 0.675331 6:-85210874 MsG0680034294.01.T01:intron
ACACACGTAAGAATAGGTAA+CGG 0.686084 6:+85210588 None:intergenic
TAACTATATACCAACAACAG+AGG 0.703519 6:-85211051 MsG0680034294.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAAGAAAATTGACAACGCT+TGG - Chr6:85210897-85210916 MsG0680034294.01.T01:CDS 30.0%
AAACAGTTTGAGCAGAAAAT+AGG - Chr6:85210800-85210819 MsG0680034294.01.T01:intron 30.0%
CACATATCAACAATATCAAG+TGG - Chr6:85210527-85210546 MsG0680034294.01.T01:CDS 30.0%
GAGGGAATATGATTTGAATA+GGG - Chr6:85210853-85210872 MsG0680034294.01.T01:intron 30.0%
GTACTTGGAATGTTATATGA+GGG - Chr6:85210835-85210854 MsG0680034294.01.T01:intron 30.0%
TAACTATATACCAACAACAG+AGG - Chr6:85210496-85210515 MsG0680034294.01.T01:CDS 30.0%
TGAGGGAATATGATTTGAAT+AGG - Chr6:85210852-85210871 MsG0680034294.01.T01:intron 30.0%
! TTTCTTTTGGCATTCTTGAA+TGG + Chr6:85210885-85210904 None:intergenic 30.0%
!! AAGCGTTGTCAATTTTCTTT+TGG + Chr6:85210898-85210917 None:intergenic 30.0%
ACACACGTAAGAATAGGTAA+CGG + Chr6:85210962-85210981 None:intergenic 35.0%
ACGACTTCTTACATTGGAAT+GGG - Chr6:85210566-85210585 MsG0680034294.01.T01:CDS 35.0%
CGCTTGGAAAGATATAAATC+AGG - Chr6:85210913-85210932 MsG0680034294.01.T01:CDS 35.0%
CGTACTTGGAATGTTATATG+AGG - Chr6:85210834-85210853 MsG0680034294.01.T01:intron 35.0%
GACAAAAGACATCTTTGACT+GGG - Chr6:85210598-85210617 MsG0680034294.01.T01:CDS 35.0%
TACGACTTCTTACATTGGAA+TGG - Chr6:85210565-85210584 MsG0680034294.01.T01:CDS 35.0%
TTGAGAACACACGTAAGAAT+AGG + Chr6:85210968-85210987 None:intergenic 35.0%
! GCAGCAGCATTAACAATTTT+TGG + Chr6:85210635-85210654 None:intergenic 35.0%
!! CAATCTCTCACGTTTTATTG+AGG - Chr6:85210985-85211004 MsG0680034294.01.T01:CDS 35.0%
AGGACATGTTTCTTCGTACT+TGG - Chr6:85210820-85210839 MsG0680034294.01.T01:intron 40.0%
ATAGTTAGCATTCAGCCATC+TGG + Chr6:85210483-85210502 None:intergenic 40.0%
CGACAAAAGACATCTTTGAC+TGG - Chr6:85210597-85210616 MsG0680034294.01.T01:CDS 40.0%
GATAACTACACACACTCTGA+CGG - Chr6:85211025-85211044 MsG0680034294.01.T01:CDS 40.0%
TGCTATACATTGCAGGCTTA+GGG - Chr6:85210652-85210671 MsG0680034294.01.T01:CDS 40.0%
TTGGCTACGACTTCTTACAT+TGG - Chr6:85210560-85210579 MsG0680034294.01.T01:CDS 40.0%
!! ATAGGTAACGGAACTTCAGT+TGG + Chr6:85210950-85210969 None:intergenic 40.0%
AAGAAGTCGTAGCCAACACT+GGG + Chr6:85210556-85210575 None:intergenic 45.0%
ATCAAGTGGTTACCCAGTGT+TGG - Chr6:85210541-85210560 MsG0680034294.01.T01:CDS 45.0%
ATGCTGCTGCTATACATTGC+AGG - Chr6:85210645-85210664 MsG0680034294.01.T01:CDS 45.0%
CTGCTATACATTGCAGGCTT+AGG - Chr6:85210651-85210670 MsG0680034294.01.T01:CDS 45.0%
GGATGAAATTGTGTCAAGCG+AGG - Chr6:85210673-85210692 MsG0680034294.01.T01:CDS 45.0%
TAAGAAGTCGTAGCCAACAC+TGG + Chr6:85210557-85210576 None:intergenic 45.0%
TGCATGTACTCCTCTGTTGT+TGG + Chr6:85210509-85210528 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr6 gene 85210470 85211099 85210470 ID=MsG0680034294.01;Name=MsG0680034294.01
Chr6 mRNA 85210470 85211099 85210470 ID=MsG0680034294.01.T01;Parent=MsG0680034294.01;Name=MsG0680034294.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|172
Chr6 exon 85210875 85211099 85210875 ID=MsG0680034294.01.T01:exon:17747;Parent=MsG0680034294.01.T01
Chr6 exon 85210470 85210763 85210470 ID=MsG0680034294.01.T01:exon:17746;Parent=MsG0680034294.01.T01
Chr6 CDS 85210875 85211099 85210875 ID=MsG0680034294.01.T01:cds;Parent=MsG0680034294.01.T01
Chr6 CDS 85210470 85210763 85210470 ID=MsG0680034294.01.T01:cds;Parent=MsG0680034294.01.T01
Gene Sequence

>MsG0680034294.01.T01

ATGAGTGAGGCCAGATGGCTGAATGCTAACTATATACCAACAACAGAGGAGTACATGCACATATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTCTTACATTGGAATGGGAAACATAGCGACAAAAGACATCTTTGACTGGGTAACTAACAATCCAAAAATTGTTAATGCTGCTGCTATACATTGCAGGCTTAGGGATGAAATTGTGTCAAGCGAGTTTGAGCAGAAAATAGGACATGTTTCTTCGTACTTGGAATGTTATATGAGGGAATATGATTTGAATAGGGAAGCTGCCATTCAAGAATGCCAAAAGAAAATTGACAACGCTTGGAAAGATATAAATCAGGAATGTCTTAAGCCAACTGAAGTTCCGTTACCTATTCTTACGTGTGTTCTCAATCTCTCACGTTTTATTGAGGTTTTTTACAAAGATACAGATAACTACACACACTCTGACGGACTAATGAAGACATATATTAATGATTTGCTTGTCAATCCCGTAGAAATTTGA

Protein sequence

>MsG0680034294.01.T01

MSEARWLNANYIPTTEEYMHISTISSGYPVLATTSYIGMGNIATKDIFDWVTNNPKIVNAAAIHCRLRDEIVSSEFEQKIGHVSSYLECYMREYDLNREAAIQECQKKIDNAWKDINQECLKPTEVPLPILTCVLNLSRFIEVFYKDTDNYTHSDGLMKTYINDLLVNPVEI*