Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034385.01.T01 | XP_003619740.2 | 96.084 | 332 | 13 | 0 | 1 | 332 | 25 | 356 | 0 | 666 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034385.01.T01 | Q9LT10 | 53.012 | 332 | 143 | 5 | 6 | 333 | 10 | 332 | 4.06E-127 | 370 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034385.01.T01 | A0A396HHZ3 | 96.084 | 332 | 13 | 0 | 1 | 332 | 25 | 356 | 0.0 | 666 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680034385.01 | MsG0880046135.01 | -0.802387 | 5.747117e-49 | 2.811722e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034385.01.T01 | MTR_6g065430 | 96.084 | 332 | 13 | 0 | 1 | 332 | 1 | 332 | 0.0 | 664 |
| MsG0680034385.01.T01 | MTR_6g065120 | 60.182 | 329 | 121 | 2 | 9 | 329 | 11 | 337 | 1.36e-148 | 422 |
| MsG0680034385.01.T01 | MTR_6g065370 | 58.084 | 334 | 131 | 2 | 3 | 329 | 4 | 335 | 2.77e-143 | 408 |
| MsG0680034385.01.T01 | MTR_6g065400 | 59.146 | 328 | 125 | 2 | 9 | 329 | 10 | 335 | 9.63e-143 | 407 |
| MsG0680034385.01.T01 | MTR_6g065270 | 56.509 | 338 | 134 | 2 | 3 | 329 | 2 | 337 | 2.89e-142 | 406 |
| MsG0680034385.01.T01 | MTR_6g065110 | 54.386 | 342 | 145 | 3 | 1 | 333 | 1 | 340 | 1.35e-137 | 394 |
| MsG0680034385.01.T01 | MTR_6g065340 | 54.079 | 331 | 127 | 3 | 6 | 329 | 18 | 330 | 4.67e-124 | 359 |
| MsG0680034385.01.T01 | MTR_1g089310 | 36.499 | 337 | 196 | 5 | 3 | 327 | 26 | 356 | 1.27e-67 | 216 |
| MsG0680034385.01.T01 | MTR_1g089310 | 36.499 | 337 | 196 | 5 | 3 | 327 | 10 | 340 | 1.65e-67 | 215 |
| MsG0680034385.01.T01 | MTR_1g082210 | 36.012 | 336 | 199 | 4 | 3 | 327 | 10 | 340 | 4.61e-67 | 214 |
| MsG0680034385.01.T01 | MTR_7g093950 | 35.000 | 340 | 203 | 5 | 3 | 327 | 10 | 346 | 4.66e-66 | 212 |
| MsG0680034385.01.T01 | MTR_6g464280 | 38.434 | 281 | 160 | 6 | 58 | 327 | 46 | 324 | 1.89e-60 | 196 |
| MsG0680034385.01.T01 | MTR_6g464510 | 35.691 | 311 | 186 | 8 | 25 | 327 | 22 | 326 | 4.42e-51 | 172 |
| MsG0680034385.01.T01 | MTR_6g464320 | 38.153 | 249 | 142 | 5 | 58 | 295 | 47 | 294 | 6.71e-51 | 172 |
| MsG0680034385.01.T01 | MTR_8g035540 | 32.915 | 319 | 194 | 6 | 26 | 331 | 9 | 320 | 1.98e-49 | 168 |
| MsG0680034385.01.T01 | MTR_8g035520 | 34.948 | 289 | 173 | 5 | 50 | 327 | 40 | 324 | 5.58e-49 | 167 |
| MsG0680034385.01.T01 | MTR_0007s0520 | 35.714 | 294 | 163 | 9 | 21 | 300 | 16 | 297 | 3.23e-48 | 165 |
| MsG0680034385.01.T01 | MTR_7g406880 | 34.304 | 309 | 177 | 8 | 45 | 327 | 41 | 349 | 8.61e-48 | 164 |
| MsG0680034385.01.T01 | MTR_1g102160 | 36.071 | 280 | 159 | 8 | 26 | 292 | 19 | 291 | 2.67e-47 | 162 |
| MsG0680034385.01.T01 | MTR_1g102170 | 35.517 | 290 | 163 | 11 | 31 | 308 | 43 | 320 | 1.67e-45 | 158 |
| MsG0680034385.01.T01 | MTR_6g464120 | 35.769 | 260 | 146 | 8 | 31 | 279 | 29 | 278 | 2.80e-45 | 162 |
| MsG0680034385.01.T01 | MTR_1g111280 | 35.338 | 266 | 150 | 8 | 57 | 307 | 60 | 318 | 2.06e-42 | 150 |
| MsG0680034385.01.T01 | MTR_1g012430 | 31.803 | 305 | 193 | 7 | 30 | 329 | 15 | 309 | 2.39e-42 | 149 |
| MsG0680034385.01.T01 | MTR_4g050140 | 32.130 | 277 | 177 | 4 | 62 | 329 | 43 | 317 | 4.49e-42 | 149 |
| MsG0680034385.01.T01 | MTR_7g107040 | 31.338 | 284 | 181 | 5 | 56 | 329 | 39 | 318 | 5.05e-42 | 149 |
| MsG0680034385.01.T01 | MTR_3g012440 | 33.948 | 271 | 164 | 5 | 74 | 330 | 74 | 343 | 2.15e-41 | 147 |
| MsG0680034385.01.T01 | MTR_8g035500 | 32.979 | 282 | 172 | 7 | 26 | 296 | 12 | 287 | 6.34e-41 | 145 |
| MsG0680034385.01.T01 | MTR_4g086480 | 33.673 | 294 | 171 | 8 | 46 | 327 | 37 | 318 | 1.52e-37 | 137 |
| MsG0680034385.01.T01 | MTR_4g086510 | 31.715 | 309 | 187 | 9 | 31 | 327 | 21 | 317 | 1.59e-36 | 134 |
| MsG0680034385.01.T01 | MTR_7g056207 | 31.250 | 288 | 183 | 6 | 50 | 327 | 67 | 349 | 3.02e-34 | 129 |
| MsG0680034385.01.T01 | MTR_4g086470 | 32.872 | 289 | 178 | 6 | 50 | 329 | 38 | 319 | 2.06e-32 | 123 |
| MsG0680034385.01.T01 | MTR_4g086540 | 29.930 | 284 | 171 | 8 | 51 | 329 | 46 | 306 | 3.43e-32 | 122 |
| MsG0680034385.01.T01 | MTR_3g101420 | 27.797 | 295 | 182 | 7 | 50 | 327 | 34 | 314 | 1.94e-28 | 112 |
| MsG0680034385.01.T01 | MTR_5g005850 | 28.931 | 318 | 192 | 12 | 31 | 328 | 26 | 329 | 5.36e-28 | 111 |
| MsG0680034385.01.T01 | MTR_1g105020 | 27.746 | 346 | 209 | 11 | 1 | 328 | 1 | 323 | 4.10e-27 | 108 |
| MsG0680034385.01.T01 | MTR_3g450680 | 26.480 | 321 | 201 | 10 | 30 | 330 | 28 | 333 | 5.15e-27 | 108 |
| MsG0680034385.01.T01 | MTR_6g022710 | 30.350 | 257 | 139 | 5 | 89 | 307 | 160 | 414 | 1.61e-26 | 109 |
| MsG0680034385.01.T01 | MTR_8g040860 | 28.169 | 284 | 172 | 7 | 30 | 297 | 28 | 295 | 1.04e-25 | 105 |
| MsG0680034385.01.T01 | MTR_6g065470 | 40.449 | 178 | 65 | 5 | 1 | 153 | 1 | 162 | 4.45e-25 | 99.8 |
| MsG0680034385.01.T01 | MTR_5g005920 | 26.582 | 316 | 204 | 11 | 30 | 327 | 25 | 330 | 6.13e-25 | 103 |
| MsG0680034385.01.T01 | MTR_1g104930 | 31.541 | 279 | 165 | 8 | 26 | 292 | 20 | 284 | 8.02e-25 | 102 |
| MsG0680034385.01.T01 | MTR_7g051020 | 29.560 | 318 | 196 | 10 | 26 | 327 | 20 | 325 | 1.18e-23 | 99.8 |
| MsG0680034385.01.T01 | MTR_5g005900 | 27.778 | 288 | 186 | 11 | 18 | 291 | 8 | 287 | 2.00e-21 | 93.6 |
| MsG0680034385.01.T01 | MTR_0007s0480 | 37.162 | 148 | 76 | 5 | 165 | 300 | 1 | 143 | 9.41e-21 | 88.2 |
| MsG0680034385.01.T01 | MTR_6g065480 | 65.517 | 58 | 19 | 1 | 227 | 284 | 1 | 57 | 6.69e-20 | 82.4 |
| MsG0680034385.01.T01 | MTR_1g104933 | 30.738 | 244 | 147 | 6 | 60 | 292 | 46 | 278 | 3.47e-19 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034385.01.T01 | AT5G23530 | 53.012 | 332 | 143 | 5 | 6 | 333 | 10 | 332 | 4.14e-128 | 370 |
| MsG0680034385.01.T01 | AT5G27320 | 37.618 | 319 | 184 | 3 | 4 | 312 | 11 | 324 | 4.72e-70 | 222 |
| MsG0680034385.01.T01 | AT3G05120 | 34.821 | 336 | 202 | 3 | 4 | 327 | 11 | 341 | 7.07e-62 | 201 |
| MsG0680034385.01.T01 | AT3G63010 | 34.615 | 338 | 202 | 4 | 3 | 327 | 10 | 341 | 8.66e-62 | 201 |
| MsG0680034385.01.T01 | AT1G68620 | 44.053 | 227 | 118 | 5 | 58 | 279 | 58 | 280 | 3.05e-56 | 186 |
| MsG0680034385.01.T01 | AT5G62180 | 37.809 | 283 | 165 | 7 | 22 | 295 | 12 | 292 | 3.98e-53 | 177 |
| MsG0680034385.01.T01 | AT2G45600 | 35.647 | 317 | 174 | 10 | 25 | 327 | 15 | 315 | 3.63e-48 | 165 |
| MsG0680034385.01.T01 | AT5G06570 | 36.656 | 311 | 177 | 10 | 30 | 327 | 22 | 325 | 5.80e-45 | 156 |
| MsG0680034385.01.T01 | AT5G06570 | 36.656 | 311 | 177 | 10 | 30 | 327 | 22 | 325 | 5.80e-45 | 156 |
| MsG0680034385.01.T01 | AT5G06570 | 36.656 | 311 | 177 | 10 | 30 | 327 | 37 | 340 | 6.36e-45 | 157 |
| MsG0680034385.01.T01 | AT5G06570 | 36.656 | 311 | 177 | 10 | 30 | 327 | 37 | 340 | 6.36e-45 | 157 |
| MsG0680034385.01.T01 | AT5G16080 | 33.948 | 271 | 161 | 8 | 70 | 329 | 73 | 336 | 1.67e-43 | 153 |
| MsG0680034385.01.T01 | AT2G45610 | 31.095 | 283 | 179 | 5 | 25 | 296 | 16 | 293 | 2.21e-36 | 134 |
| MsG0680034385.01.T01 | AT1G47480 | 31.273 | 275 | 178 | 6 | 50 | 320 | 35 | 302 | 1.93e-35 | 131 |
| MsG0680034385.01.T01 | AT3G48700 | 30.097 | 309 | 176 | 10 | 46 | 327 | 30 | 325 | 2.82e-33 | 125 |
| MsG0680034385.01.T01 | AT1G19190 | 30.032 | 313 | 192 | 10 | 31 | 330 | 18 | 316 | 1.52e-32 | 123 |
| MsG0680034385.01.T01 | AT2G03550 | 31.615 | 291 | 178 | 8 | 50 | 329 | 32 | 312 | 7.12e-32 | 121 |
| MsG0680034385.01.T01 | AT1G49650 | 35.223 | 247 | 144 | 8 | 56 | 292 | 94 | 334 | 1.25e-29 | 117 |
| MsG0680034385.01.T01 | AT3G48690 | 30.769 | 260 | 154 | 6 | 50 | 292 | 32 | 282 | 1.88e-29 | 115 |
| MsG0680034385.01.T01 | AT5G14310 | 30.169 | 295 | 161 | 5 | 76 | 328 | 140 | 431 | 5.84e-29 | 116 |
| MsG0680034385.01.T01 | AT1G49660 | 34.156 | 243 | 147 | 6 | 57 | 290 | 39 | 277 | 8.38e-29 | 113 |
| MsG0680034385.01.T01 | AT3G27320 | 30.033 | 303 | 157 | 8 | 73 | 328 | 119 | 413 | 5.49e-28 | 113 |
| MsG0680034385.01.T01 | AT3G27320 | 30.033 | 303 | 157 | 8 | 73 | 328 | 151 | 445 | 6.83e-28 | 113 |
| MsG0680034385.01.T01 | AT1G49640 | 33.186 | 226 | 139 | 8 | 56 | 275 | 39 | 258 | 1.38e-27 | 110 |
Find 94 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGTTGATGGATTTGGTATT+AGG | 0.243759 | 6:-86718051 | None:intergenic |
| CCTGTTATTATATTCTTTCA+TGG | 0.259099 | 6:+86718171 | MsG0680034385.01.T01:CDS |
| AATCTTTGACTTTAGAAATA+AGG | 0.277895 | 6:-86718857 | None:intergenic |
| GGCTGCTCGGGTTTCCTTAG+AGG | 0.291006 | 6:+86718452 | MsG0680034385.01.T01:CDS |
| TATGAGTGGTTGAGAAAATC+TGG | 0.304072 | 6:+86718756 | MsG0680034385.01.T01:CDS |
| ATTGTTGTCTCTGTTAATTA+TGG | 0.310023 | 6:+86718273 | MsG0680034385.01.T01:CDS |
| CGTTGATGGATTTGGTATTA+GGG | 0.321201 | 6:-86718050 | None:intergenic |
| TTTCAAACCATCCTCGTATT+GGG | 0.330000 | 6:-86718322 | None:intergenic |
| AGAGACGTCGTTGATGGATT+TGG | 0.342080 | 6:-86718058 | None:intergenic |
| ATGGTCTCGGTTTGATCCAT+TGG | 0.345507 | 6:-86718619 | None:intergenic |
| TTTGAAATATCCATTGCATT+AGG | 0.348684 | 6:-86718663 | None:intergenic |
| TGTTGTGGTGGCAGTGGTGG+TGG | 0.349827 | 6:-86718142 | None:intergenic |
| TCACAAGTCTCGGGCAAATC+AGG | 0.355571 | 6:-86718834 | None:intergenic |
| TGGATCCCACATGCAACCTT+TGG | 0.371659 | 6:+86718097 | MsG0680034385.01.T01:CDS |
| ATGGATTTGGTATTAGGGTT+GGG | 0.372159 | 6:-86718045 | None:intergenic |
| GGCCCATCATGTGGCTGCTC+GGG | 0.376483 | 6:+86718440 | MsG0680034385.01.T01:CDS |
| ATTATATTCTTTCATGGTGG+TGG | 0.385738 | 6:+86718177 | MsG0680034385.01.T01:CDS |
| TGGGATGGAAAACGATGTTC+GGG | 0.389712 | 6:-86718303 | None:intergenic |
| CACAAACATTAGACGCTTCA+TGG | 0.393644 | 6:-86718638 | None:intergenic |
| TAAAACCCGAAAATCCTCTA+AGG | 0.397817 | 6:-86718466 | None:intergenic |
| AGAGGAAGAGACGTCGTTGA+TGG | 0.398956 | 6:-86718064 | None:intergenic |
| TTGGGATGGAAAACGATGTT+CGG | 0.400620 | 6:-86718304 | None:intergenic |
| TGGATAAAACCGATTGGTAT+TGG | 0.404311 | 6:+86718583 | MsG0680034385.01.T01:CDS |
| CCAACACACACCAGGGTATT+CGG | 0.413266 | 6:-86718696 | None:intergenic |
| TGGCCCATCATGTGGCTGCT+CGG | 0.418887 | 6:+86718439 | MsG0680034385.01.T01:CDS |
| AGAAATAAGGTCACAAGTCT+CGG | 0.419991 | 6:-86718844 | None:intergenic |
| TTTCCATGGTAGGCGTCGTT+TGG | 0.420934 | 6:-86717917 | None:intergenic |
| ACGACCGGTGATATCGCTTA+TGG | 0.421888 | 6:-86717968 | None:intergenic |
| TGGTCTCGGTTTGATCCATT+GGG | 0.433211 | 6:-86718618 | None:intergenic |
| CCATGAAAGAATATAATAAC+AGG | 0.440173 | 6:-86718171 | None:intergenic |
| GCTGAGGAGGCAGCGGTTGA+TGG | 0.441670 | 6:-86718001 | None:intergenic |
| GATGGATTTGGTATTAGGGT+TGG | 0.442855 | 6:-86718046 | None:intergenic |
| GTGGCAGTGGTGGTGGAGGA+AGG | 0.443472 | 6:-86718135 | None:intergenic |
| CATTAGACGCTTCATGGTCT+CGG | 0.451073 | 6:-86718632 | None:intergenic |
| TGTGGTGGCAGTGGTGGTGG+AGG | 0.461859 | 6:-86718139 | None:intergenic |
| TGATAGTGCGGGTGGAAACT+TGG | 0.463277 | 6:+86718419 | MsG0680034385.01.T01:CDS |
| GCCTCCTCAGCTTCATTGAC+TGG | 0.475494 | 6:+86718013 | MsG0680034385.01.T01:CDS |
| GGTGTGACCCGTTAGTAGAT+TGG | 0.487804 | 6:+86718721 | MsG0680034385.01.T01:CDS |
| AGATGTTGTGGTGGCAGTGG+TGG | 0.491942 | 6:-86718145 | None:intergenic |
| AAACCCGAGCAGCCACATGA+TGG | 0.493875 | 6:-86718443 | None:intergenic |
| ATCGCTTATGGCGGAGAGGA+TGG | 0.494027 | 6:-86717956 | None:intergenic |
| GCAGCGGTTGATGGTGCCAT+TGG | 0.496036 | 6:-86717992 | None:intergenic |
| CTCTTCCTCTGACGTCGTTG+TGG | 0.501380 | 6:+86718077 | MsG0680034385.01.T01:CDS |
| TCGTTTGGTTTCTGAGGCCA+TGG | 0.505951 | 6:-86717902 | None:intergenic |
| GAAATAAGGTCACAAGTCTC+GGG | 0.507371 | 6:-86718843 | None:intergenic |
| AACCCGAGCAGCCACATGAT+GGG | 0.509075 | 6:-86718442 | None:intergenic |
| ACACGTACCCATTTGTTCGA+TGG | 0.513184 | 6:+86718563 | MsG0680034385.01.T01:CDS |
| CATGAAGCGTCTAATGTTTG+TGG | 0.519918 | 6:+86718639 | MsG0680034385.01.T01:CDS |
| AATACCCTGGTGTGTGTTGG+TGG | 0.520657 | 6:+86718699 | MsG0680034385.01.T01:CDS |
| CGTTAGTAGATTGGCAAAAG+AGG | 0.521206 | 6:+86718730 | MsG0680034385.01.T01:CDS |
| AAACATCTTCCAATACCAAT+CGG | 0.522761 | 6:-86718592 | None:intergenic |
| CACACCCACCAACACACACC+AGG | 0.523330 | 6:-86718704 | None:intergenic |
| AAACGACAAAGCATGTGGTA+TGG | 0.528057 | 6:-86718228 | None:intergenic |
| ATGAAGCGTCTAATGTTTGT+GGG | 0.531000 | 6:+86718640 | MsG0680034385.01.T01:CDS |
| ATGAGTGGTTGAGAAAATCT+GGG | 0.547195 | 6:+86718757 | MsG0680034385.01.T01:CDS |
| AAACCATCCTCGTATTGGGA+TGG | 0.547219 | 6:-86718318 | None:intergenic |
| ATGTGGTATGGAATAGAAGA+TGG | 0.558323 | 6:-86718216 | None:intergenic |
| GATATCACCGGTCGTTCCAA+TGG | 0.560623 | 6:+86717976 | MsG0680034385.01.T01:CDS |
| CAATGAAGCTGAGGAGGCAG+CGG | 0.560885 | 6:-86718008 | None:intergenic |
| GTAGAGAAGCGCACTTTCCA+TGG | 0.562398 | 6:-86717931 | None:intergenic |
| AAGAGATGTTGTGGTGGCAG+TGG | 0.562790 | 6:-86718148 | None:intergenic |
| TTTCCATCCCAATACGAGGA+TGG | 0.569261 | 6:+86718315 | MsG0680034385.01.T01:CDS |
| TCCAGTCAATGAAGCTGAGG+AGG | 0.571956 | 6:-86718014 | None:intergenic |
| GTTCGATGGATAAAACCGAT+TGG | 0.574702 | 6:+86718577 | MsG0680034385.01.T01:CDS |
| TAGGCGTCGTTTGGTTTCTG+AGG | 0.577198 | 6:-86717908 | None:intergenic |
| AAACCAAACGACGCCTACCA+TGG | 0.586056 | 6:+86717914 | MsG0680034385.01.T01:CDS |
| TTAACAGAGACAACAATGGC+AGG | 0.589799 | 6:-86718267 | None:intergenic |
| GTTATTATATTCTTTCATGG+TGG | 0.594482 | 6:+86718174 | MsG0680034385.01.T01:CDS |
| AGAAGCGCACTTTCCATGGT+AGG | 0.601167 | 6:-86717927 | None:intergenic |
| GGCAAAAGAGGTATTATGAG+TGG | 0.601718 | 6:+86718742 | MsG0680034385.01.T01:CDS |
| AATAACAGGAAGAGATGTTG+TGG | 0.603963 | 6:-86718157 | None:intergenic |
| ACGAAACCAAAGGTTGCATG+TGG | 0.607119 | 6:-86718103 | None:intergenic |
| GATTTGGTATTAGGGTTGGG+AGG | 0.609436 | 6:-86718042 | None:intergenic |
| CCGAATACCCTGGTGTGTGT+TGG | 0.610909 | 6:+86718696 | MsG0680034385.01.T01:CDS |
| GATGGTGCCATTGGAACGAC+CGG | 0.616054 | 6:-86717983 | None:intergenic |
| ATACCCTGGTGTGTGTTGGT+GGG | 0.617422 | 6:+86718700 | MsG0680034385.01.T01:CDS |
| TTGGCGGGTGATAGTGCGGG+TGG | 0.621623 | 6:+86718411 | MsG0680034385.01.T01:CDS |
| TGTTTGTGGGCCTAATGCAA+TGG | 0.628106 | 6:+86718653 | MsG0680034385.01.T01:CDS |
| GGGATCCACAACGACGTCAG+AGG | 0.640186 | 6:-86718082 | None:intergenic |
| AAAATAAACGACAAAGCATG+TGG | 0.641574 | 6:-86718233 | None:intergenic |
| TGGAAACTTGGCCCATCATG+TGG | 0.642649 | 6:+86718431 | MsG0680034385.01.T01:CDS |
| GGATGGAAAACGATGTTCGG+GGG | 0.645622 | 6:-86718301 | None:intergenic |
| TTTCAATCGAATCTCAGACT+CGG | 0.646134 | 6:-86718541 | None:intergenic |
| GAATGAAGAGACGAAACCAA+AGG | 0.650225 | 6:-86718113 | None:intergenic |
| AACAGGAAGAGATGTTGTGG+TGG | 0.654794 | 6:-86718154 | None:intergenic |
| GCTAATAGAATATCCAAACA+TGG | 0.673291 | 6:+86718791 | MsG0680034385.01.T01:CDS |
| ACCGGTGATATCGCTTATGG+CGG | 0.673748 | 6:-86717965 | None:intergenic |
| TCCGCCATAAGCGATATCAC+CGG | 0.679978 | 6:+86717964 | MsG0680034385.01.T01:CDS |
| CGAAACCAAAGGTTGCATGT+GGG | 0.684263 | 6:-86718102 | None:intergenic |
| TGTCGATTATCCGAATACCC+TGG | 0.688446 | 6:+86718686 | MsG0680034385.01.T01:CDS |
| ATAATTAACAGAGACAACAA+TGG | 0.692725 | 6:-86718271 | None:intergenic |
| TGATATCGCTTATGGCGGAG+AGG | 0.700015 | 6:-86717960 | None:intergenic |
| ACACCCACCAACACACACCA+GGG | 0.703275 | 6:-86718703 | None:intergenic |
| GGGATGGAAAACGATGTTCG+GGG | 0.713000 | 6:-86718302 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCTTTGACTTTAGAAATA+AGG | - | Chr6:86718860-86718879 | None:intergenic | 20.0% |
| ! | ATAATTAACAGAGACAACAA+TGG | - | Chr6:86718274-86718293 | None:intergenic | 25.0% |
| ! | ATTGTTGTCTCTGTTAATTA+TGG | + | Chr6:86718273-86718292 | MsG0680034385.01.T01:CDS | 25.0% |
| ! | CCATGAAAGAATATAATAAC+AGG | - | Chr6:86718174-86718193 | None:intergenic | 25.0% |
| ! | CCTGTTATTATATTCTTTCA+TGG | + | Chr6:86718171-86718190 | MsG0680034385.01.T01:CDS | 25.0% |
| ! | GTTATTATATTCTTTCATGG+TGG | + | Chr6:86718174-86718193 | MsG0680034385.01.T01:CDS | 25.0% |
| !! | AGATTTTATGATCAAGCAAA+TGG | + | Chr6:86718875-86718894 | MsG0680034385.01.T01:CDS | 25.0% |
| !! | GATCAAAATAGTGATGTTTT+AGG | + | Chr6:86718360-86718379 | MsG0680034385.01.T01:CDS | 25.0% |
| !! | TTTGAAATATCCATTGCATT+AGG | - | Chr6:86718666-86718685 | None:intergenic | 25.0% |
| !!! | AAACATCACTATTTTGATCT+AGG | - | Chr6:86718359-86718378 | None:intergenic | 25.0% |
| !!! | TATTTTGATCTAGGAACTTA+AGG | - | Chr6:86718350-86718369 | None:intergenic | 25.0% |
| AAAATAAACGACAAAGCATG+TGG | - | Chr6:86718236-86718255 | None:intergenic | 30.0% | |
| AAACATCTTCCAATACCAAT+CGG | - | Chr6:86718595-86718614 | None:intergenic | 30.0% | |
| ATTATATTCTTTCATGGTGG+TGG | + | Chr6:86718177-86718196 | MsG0680034385.01.T01:CDS | 30.0% | |
| GCTAATAGAATATCCAAACA+TGG | + | Chr6:86718791-86718810 | MsG0680034385.01.T01:CDS | 30.0% | |
| ! | AAAAAAGCATGAACCATGTT+TGG | - | Chr6:86718807-86718826 | None:intergenic | 30.0% |
| ! | GTTTTATCCATCGAACAAAT+GGG | - | Chr6:86718573-86718592 | None:intergenic | 30.0% |
| !!! | GGTTTTAAAGGTTATTGGAT+TGG | + | Chr6:86718482-86718501 | MsG0680034385.01.T01:CDS | 30.0% |
| !!! | TGACATCAGCAAATGTTTTT+TGG | + | Chr6:86718392-86718411 | MsG0680034385.01.T01:CDS | 30.0% |
| !!! | TTTCGGGTTTTAAAGGTTAT+TGG | + | Chr6:86718477-86718496 | MsG0680034385.01.T01:CDS | 30.0% |
| AATAACAGGAAGAGATGTTG+TGG | - | Chr6:86718160-86718179 | None:intergenic | 35.0% | |
| AGAAATAAGGTCACAAGTCT+CGG | - | Chr6:86718847-86718866 | None:intergenic | 35.0% | |
| ATGAGTGGTTGAGAAAATCT+GGG | + | Chr6:86718757-86718776 | MsG0680034385.01.T01:CDS | 35.0% | |
| ATGTGGTATGGAATAGAAGA+TGG | - | Chr6:86718219-86718238 | None:intergenic | 35.0% | |
| TAAAACCCGAAAATCCTCTA+AGG | - | Chr6:86718469-86718488 | None:intergenic | 35.0% | |
| TATGAGTGGTTGAGAAAATC+TGG | + | Chr6:86718756-86718775 | MsG0680034385.01.T01:CDS | 35.0% | |
| TGGATAAAACCGATTGGTAT+TGG | + | Chr6:86718583-86718602 | MsG0680034385.01.T01:CDS | 35.0% | |
| TTTCAAACCATCCTCGTATT+GGG | - | Chr6:86718325-86718344 | None:intergenic | 35.0% | |
| TTTCAATCGAATCTCAGACT+CGG | - | Chr6:86718544-86718563 | None:intergenic | 35.0% | |
| ! | ATGAAGCGTCTAATGTTTGT+GGG | + | Chr6:86718640-86718659 | MsG0680034385.01.T01:CDS | 35.0% |
| ! | ATGGATTTGGTATTAGGGTT+GGG | - | Chr6:86718048-86718067 | None:intergenic | 35.0% |
| ! | CTCTTTTGCCAATCTACTAA+CGG | - | Chr6:86718732-86718751 | None:intergenic | 35.0% |
| ! | TCTTTTGCCAATCTACTAAC+GGG | - | Chr6:86718731-86718750 | None:intergenic | 35.0% |
| ! | TTTTCAAACCATCCTCGTAT+TGG | - | Chr6:86718326-86718345 | None:intergenic | 35.0% |
| !! | CGTTGATGGATTTGGTATTA+GGG | - | Chr6:86718053-86718072 | None:intergenic | 35.0% |
| !! | GGTTTTATCCATCGAACAAA+TGG | - | Chr6:86718574-86718593 | None:intergenic | 35.0% |
| !! | TCGTTGATGGATTTGGTATT+AGG | - | Chr6:86718054-86718073 | None:intergenic | 35.0% |
| !!! | AGAGGATTTTCGGGTTTTAA+AGG | + | Chr6:86718470-86718489 | MsG0680034385.01.T01:CDS | 35.0% |
| !!! | ATCAGCAAATGTTTTTTGGC+GGG | + | Chr6:86718396-86718415 | MsG0680034385.01.T01:CDS | 35.0% |
| !!! | CATCAGCAAATGTTTTTTGG+CGG | + | Chr6:86718395-86718414 | MsG0680034385.01.T01:CDS | 35.0% |
| !!! | GTTTCGATCCAACCTTTTTT+TGG | + | Chr6:86718504-86718523 | MsG0680034385.01.T01:CDS | 35.0% |
| AAACGACAAAGCATGTGGTA+TGG | - | Chr6:86718231-86718250 | None:intergenic | 40.0% | |
| CACAAACATTAGACGCTTCA+TGG | - | Chr6:86718641-86718660 | None:intergenic | 40.0% | |
| CGTTAGTAGATTGGCAAAAG+AGG | + | Chr6:86718730-86718749 | MsG0680034385.01.T01:CDS | 40.0% | |
| GAAATAAGGTCACAAGTCTC+GGG | - | Chr6:86718846-86718865 | None:intergenic | 40.0% | |
| GAATGAAGAGACGAAACCAA+AGG | - | Chr6:86718116-86718135 | None:intergenic | 40.0% | |
| GTTCGATGGATAAAACCGAT+TGG | + | Chr6:86718577-86718596 | MsG0680034385.01.T01:CDS | 40.0% | |
| TTAACAGAGACAACAATGGC+AGG | - | Chr6:86718270-86718289 | None:intergenic | 40.0% | |
| TTGGGATGGAAAACGATGTT+CGG | - | Chr6:86718307-86718326 | None:intergenic | 40.0% | |
| ! | CATGAAGCGTCTAATGTTTG+TGG | + | Chr6:86718639-86718658 | MsG0680034385.01.T01:CDS | 40.0% |
| ! | GATGGATTTGGTATTAGGGT+TGG | - | Chr6:86718049-86718068 | None:intergenic | 40.0% |
| ! | GGGTTTCCTTAGAGGATTTT+CGG | + | Chr6:86718460-86718479 | MsG0680034385.01.T01:CDS | 40.0% |
| ! | GGTTTCCTTAGAGGATTTTC+GGG | + | Chr6:86718461-86718480 | MsG0680034385.01.T01:CDS | 40.0% |
| ! | TTTTCCAGTCAATGAAGCTG+AGG | - | Chr6:86718020-86718039 | None:intergenic | 40.0% |
| !! | GGCAAAAGAGGTATTATGAG+TGG | + | Chr6:86718742-86718761 | MsG0680034385.01.T01:CDS | 40.0% |
| !! | TGGAAGATGTTTTTGCCCAA+TGG | + | Chr6:86718603-86718622 | MsG0680034385.01.T01:CDS | 40.0% |
| !!! | TCGATCCAACCTTTTTTTGG+AGG | + | Chr6:86718507-86718526 | MsG0680034385.01.T01:CDS | 40.0% |
| AAACCATCCTCGTATTGGGA+TGG | - | Chr6:86718321-86718340 | None:intergenic | 45.0% | |
| AACAGGAAGAGATGTTGTGG+TGG | - | Chr6:86718157-86718176 | None:intergenic | 45.0% | |
| ACACGTACCCATTTGTTCGA+TGG | + | Chr6:86718563-86718582 | MsG0680034385.01.T01:CDS | 45.0% | |
| ACGAAACCAAAGGTTGCATG+TGG | - | Chr6:86718106-86718125 | None:intergenic | 45.0% | |
| CATTAGACGCTTCATGGTCT+CGG | - | Chr6:86718635-86718654 | None:intergenic | 45.0% | |
| CCTCACCTCCAAAAAAAGGT+TGG | - | Chr6:86718515-86718534 | None:intergenic | 45.0% | |
| CGAAACCAAAGGTTGCATGT+GGG | - | Chr6:86718105-86718124 | None:intergenic | 45.0% | |
| CGTTCCTCACCTCCAAAAAA+AGG | - | Chr6:86718519-86718538 | None:intergenic | 45.0% | |
| TGGGATGGAAAACGATGTTC+GGG | - | Chr6:86718306-86718325 | None:intergenic | 45.0% | |
| TGTTTGTGGGCCTAATGCAA+TGG | + | Chr6:86718653-86718672 | MsG0680034385.01.T01:CDS | 45.0% | |
| TTTCCATCCCAATACGAGGA+TGG | + | Chr6:86718315-86718334 | MsG0680034385.01.T01:CDS | 45.0% | |
| ! | ATGGTCTCGGTTTGATCCAT+TGG | - | Chr6:86718622-86718641 | None:intergenic | 45.0% |
| ! | GATTTGGTATTAGGGTTGGG+AGG | - | Chr6:86718045-86718064 | None:intergenic | 45.0% |
| ! | TCGTTTTCCATCCCAATACG+AGG | + | Chr6:86718311-86718330 | MsG0680034385.01.T01:CDS | 45.0% |
| ! | TGGTCTCGGTTTGATCCATT+GGG | - | Chr6:86718621-86718640 | None:intergenic | 45.0% |
| ! | TGTCGATTATCCGAATACCC+TGG | + | Chr6:86718686-86718705 | MsG0680034385.01.T01:CDS | 45.0% |
| !! | AGAGACGTCGTTGATGGATT+TGG | - | Chr6:86718061-86718080 | None:intergenic | 45.0% |
| !!! | CCAACCTTTTTTTGGAGGTG+AGG | + | Chr6:86718512-86718531 | MsG0680034385.01.T01:CDS | 45.0% |
| !!! | TTTTTTGGCGGGTGATAGTG+CGG | + | Chr6:86718407-86718426 | MsG0680034385.01.T01:CDS | 45.0% |
| AAACCAAACGACGCCTACCA+TGG | + | Chr6:86717914-86717933 | MsG0680034385.01.T01:CDS | 50.0% | |
| AAGAGATGTTGTGGTGGCAG+TGG | - | Chr6:86718151-86718170 | None:intergenic | 50.0% | |
| AATACCCTGGTGTGTGTTGG+TGG | + | Chr6:86718699-86718718 | MsG0680034385.01.T01:CDS | 50.0% | |
| ACCGGTGATATCGCTTATGG+CGG | - | Chr6:86717968-86717987 | None:intergenic | 50.0% | |
| ACGACCGGTGATATCGCTTA+TGG | - | Chr6:86717971-86717990 | None:intergenic | 50.0% | |
| AGAGGAAGAGACGTCGTTGA+TGG | - | Chr6:86718067-86718086 | None:intergenic | 50.0% | |
| GATATCACCGGTCGTTCCAA+TGG | + | Chr6:86717976-86717995 | MsG0680034385.01.T01:CDS | 50.0% | |
| GGATGGAAAACGATGTTCGG+GGG | - | Chr6:86718304-86718323 | None:intergenic | 50.0% | |
| GGGATGGAAAACGATGTTCG+GGG | - | Chr6:86718305-86718324 | None:intergenic | 50.0% | |
| GGTGTGACCCGTTAGTAGAT+TGG | + | Chr6:86718721-86718740 | MsG0680034385.01.T01:CDS | 50.0% | |
| TCACAAGTCTCGGGCAAATC+AGG | - | Chr6:86718837-86718856 | None:intergenic | 50.0% | |
| TCCAGTCAATGAAGCTGAGG+AGG | - | Chr6:86718017-86718036 | None:intergenic | 50.0% | |
| TCCGCCATAAGCGATATCAC+CGG | + | Chr6:86717964-86717983 | MsG0680034385.01.T01:CDS | 50.0% | |
| TGGAAACTTGGCCCATCATG+TGG | + | Chr6:86718431-86718450 | MsG0680034385.01.T01:CDS | 50.0% | |
| TGGATCCCACATGCAACCTT+TGG | + | Chr6:86718097-86718116 | MsG0680034385.01.T01:CDS | 50.0% | |
| ! | AGAAGCGCACTTTCCATGGT+AGG | - | Chr6:86717930-86717949 | None:intergenic | 50.0% |
| ! | ATACCCTGGTGTGTGTTGGT+GGG | + | Chr6:86718700-86718719 | MsG0680034385.01.T01:CDS | 50.0% |
| ! | CCAACACACACCAGGGTATT+CGG | - | Chr6:86718699-86718718 | None:intergenic | 50.0% |
| ! | GTAGAGAAGCGCACTTTCCA+TGG | - | Chr6:86717934-86717953 | None:intergenic | 50.0% |
| ! | TAGGCGTCGTTTGGTTTCTG+AGG | - | Chr6:86717911-86717930 | None:intergenic | 50.0% |
| ! | TGATAGTGCGGGTGGAAACT+TGG | + | Chr6:86718419-86718438 | MsG0680034385.01.T01:CDS | 50.0% |
| ! | TGATATCGCTTATGGCGGAG+AGG | - | Chr6:86717963-86717982 | None:intergenic | 50.0% |
| ! | TTTCCATGGTAGGCGTCGTT+TGG | - | Chr6:86717920-86717939 | None:intergenic | 50.0% |
| !! | TTTTTGGCGGGTGATAGTGC+GGG | + | Chr6:86718408-86718427 | MsG0680034385.01.T01:CDS | 50.0% |
| AAACCCGAGCAGCCACATGA+TGG | - | Chr6:86718446-86718465 | None:intergenic | 55.0% | |
| AACCCGAGCAGCCACATGAT+GGG | - | Chr6:86718445-86718464 | None:intergenic | 55.0% | |
| ACACCCACCAACACACACCA+GGG | - | Chr6:86718706-86718725 | None:intergenic | 55.0% | |
| AGATGTTGTGGTGGCAGTGG+TGG | - | Chr6:86718148-86718167 | None:intergenic | 55.0% | |
| CAATGAAGCTGAGGAGGCAG+CGG | - | Chr6:86718011-86718030 | None:intergenic | 55.0% | |
| CCGAATACCCTGGTGTGTGT+TGG | + | Chr6:86718696-86718715 | MsG0680034385.01.T01:CDS | 55.0% | |
| CTCTTCCTCTGACGTCGTTG+TGG | + | Chr6:86718077-86718096 | MsG0680034385.01.T01:CDS | 55.0% | |
| GCCTCCTCAGCTTCATTGAC+TGG | + | Chr6:86718013-86718032 | MsG0680034385.01.T01:CDS | 55.0% | |
| ! | ATCGCTTATGGCGGAGAGGA+TGG | - | Chr6:86717959-86717978 | None:intergenic | 55.0% |
| !! | GATGGTGCCATTGGAACGAC+CGG | - | Chr6:86717986-86718005 | None:intergenic | 55.0% |
| CACACCCACCAACACACACC+AGG | - | Chr6:86718707-86718726 | None:intergenic | 60.0% | |
| GGCTGCTCGGGTTTCCTTAG+AGG | + | Chr6:86718452-86718471 | MsG0680034385.01.T01:CDS | 60.0% | |
| GGGATCCACAACGACGTCAG+AGG | - | Chr6:86718085-86718104 | None:intergenic | 60.0% | |
| TGGCCCATCATGTGGCTGCT+CGG | + | Chr6:86718439-86718458 | MsG0680034385.01.T01:CDS | 60.0% | |
| TGTTGTGGTGGCAGTGGTGG+TGG | - | Chr6:86718145-86718164 | None:intergenic | 60.0% | |
| !! | GCAGCGGTTGATGGTGCCAT+TGG | - | Chr6:86717995-86718014 | None:intergenic | 60.0% |
| GCTGAGGAGGCAGCGGTTGA+TGG | - | Chr6:86718004-86718023 | None:intergenic | 65.0% | |
| GGCCCATCATGTGGCTGCTC+GGG | + | Chr6:86718440-86718459 | MsG0680034385.01.T01:CDS | 65.0% | |
| ! | TTGGCGGGTGATAGTGCGGG+TGG | + | Chr6:86718411-86718430 | MsG0680034385.01.T01:CDS | 65.0% |
| !! | GTGGCAGTGGTGGTGGAGGA+AGG | - | Chr6:86718138-86718157 | None:intergenic | 65.0% |
| !! | TGTGGTGGCAGTGGTGGTGG+AGG | - | Chr6:86718142-86718161 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 86717904 | 86718908 | 86717904 | ID=MsG0680034385.01;Name=MsG0680034385.01 |
| Chr6 | mRNA | 86717904 | 86718908 | 86717904 | ID=MsG0680034385.01.T01;Parent=MsG0680034385.01;Name=MsG0680034385.01.T01;_AED=0.12;_eAED=0.12;_QI=0|-1|0|1|-1|1|1|0|334 |
| Chr6 | exon | 86717904 | 86718908 | 86717904 | ID=MsG0680034385.01.T01:exon:18215;Parent=MsG0680034385.01.T01 |
| Chr6 | CDS | 86717904 | 86718908 | 86717904 | ID=MsG0680034385.01.T01:cds;Parent=MsG0680034385.01.T01 |
| Gene Sequence |
| Protein sequence |
