AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034834.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034834.01.T01 MTR_6g477780 96.552 145 5 0 1 145 240 384 6.13e-102 297
MsG0680034834.01.T01 MTR_5g013620 56.250 112 38 1 1 112 410 510 2.32e-36 131
MsG0680034834.01.T01 MTR_4g099110 44.218 147 74 3 1 144 399 540 1.73e-34 125
MsG0680034834.01.T01 MTR_5g013640 53.571 112 41 1 1 112 408 508 3.75e-34 125
MsG0680034834.01.T01 MTR_2g089030 41.007 139 71 4 1 136 405 535 2.22e-25 100
MsG0680034834.01.T01 MTR_4g045667 45.536 112 57 2 1 109 332 442 9.86e-25 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034834.01.T01 AT3G23920 78.378 148 29 2 1 145 428 575 1.22e-75 235
MsG0680034834.01.T01 AT4G17090 52.679 112 48 2 1 112 407 513 4.15e-32 119
MsG0680034834.01.T01 AT2G32290 36.765 136 79 2 1 136 398 526 7.82e-23 93.6
MsG0680034834.01.T01 AT5G55700 36.697 109 64 2 1 109 424 527 3.70e-20 85.9
MsG0680034834.01.T01 AT5G55700 36.364 110 65 2 1 110 261 365 8.94e-20 84.3
MsG0680034834.01.T01 AT4G15210 38.393 112 65 2 1 109 281 391 3.16e-19 83.2
MsG0680034834.01.T01 AT4G15210 38.393 112 65 2 1 109 281 391 3.16e-19 83.2
MsG0680034834.01.T01 AT4G15210 38.393 112 65 2 1 109 334 444 3.54e-19 83.2
MsG0680034834.01.T01 AT4G00490 39.823 113 61 3 1 109 413 522 9.02e-18 79.0
MsG0680034834.01.T01 AT4G15210 43.077 65 37 0 1 65 334 398 9.53e-12 61.6

Find 33 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTGCTCCCAACATTTAT+TGG 0.108972 6:-95162136 None:intergenic
CTAGATGAACCTGTGCTTTC+TGG 0.113345 6:-95161912 None:intergenic
ATTTGTTTCAACCTGATAAT+TGG 0.236175 6:+95162067 MsG0680034834.01.T01:CDS
ACAAACTTTCTCCAATTATC+AGG 0.268602 6:-95162078 None:intergenic
TGCACATGAACAGATATTAA+AGG 0.334381 6:+95161969 MsG0680034834.01.T01:CDS
TATCAGGTTGAAACAAATCT+TGG 0.371891 6:-95162062 None:intergenic
TAGATGAACCTGTGCTTTCT+GGG 0.440379 6:-95161911 None:intergenic
TTTGTGAAGAAGATGAAAGA+AGG 0.444526 6:+95162105 MsG0680034834.01.T01:CDS
ACTTGATTCACGAGCTTCTC+CGG 0.454036 6:-95161877 None:intergenic
AAGCACAGGTTCATCTAGCC+GGG 0.475765 6:+95161917 MsG0680034834.01.T01:CDS
GAAAAGTGCCAATAAATGTT+GGG 0.487450 6:+95162128 MsG0680034834.01.T01:CDS
GCGTGATCACGAGCAGCCTC+AGG 0.490422 6:+95161840 MsG0680034834.01.T01:CDS
ACAAATCTTGGTTCATCCTA+AGG 0.501427 6:-95162050 None:intergenic
ATGTTGGGAGCAAGTGGAGA+GGG 0.507668 6:+95162143 MsG0680034834.01.T01:CDS
TGTGTGCATTCACATACCTT+AGG 0.510560 6:+95162034 MsG0680034834.01.T01:CDS
GGAAAAGTGCCAATAAATGT+TGG 0.513383 6:+95162127 MsG0680034834.01.T01:CDS
TTGTGAAGAAGATGAAAGAA+GGG 0.520951 6:+95162106 MsG0680034834.01.T01:CDS
AAAGCACAGGTTCATCTAGC+CGG 0.521686 6:+95161916 MsG0680034834.01.T01:CDS
GCAACAGCTTCTTGCACAAG+AGG 0.543294 6:-95162195 None:intergenic
CAATAAATGTTGGGAGCAAG+TGG 0.588636 6:+95162137 MsG0680034834.01.T01:CDS
GCTCAAATGTTGGCGAGACA+CGG 0.591977 6:+95161787 None:intergenic
TTTAGCTACCCAGAAAGCAC+AGG 0.593582 6:+95161903 MsG0680034834.01.T01:CDS
GTTGAATGTCGAGGGTAACT+CGG 0.597129 6:+95161999 MsG0680034834.01.T01:CDS
TCAGGATGCACAATGTGCAC+CGG 0.605743 6:+95161858 MsG0680034834.01.T01:CDS
GGCGTCCGCGTTGAATGTCG+AGG 0.608236 6:+95161990 MsG0680034834.01.T01:CDS
CCGGGGAAAATGCTTTGCCA+CGG 0.638800 6:+95161935 MsG0680034834.01.T01:CDS
AATGTTGGGAGCAAGTGGAG+AGG 0.645936 6:+95162142 MsG0680034834.01.T01:CDS
GGTGCACATTGTGCATCCTG+AGG 0.646679 6:-95161856 None:intergenic
GGAGAAGCTCGTGAATCAAG+TGG 0.659808 6:+95161879 MsG0680034834.01.T01:CDS
GCGTCCGCGTTGAATGTCGA+GGG 0.669013 6:+95161991 MsG0680034834.01.T01:CDS
AGCACAGGTTCATCTAGCCG+GGG 0.672042 6:+95161918 MsG0680034834.01.T01:CDS
TGTGCATGTTCATCATACCG+TGG 0.724780 6:-95161952 None:intergenic
GTTACCCTCGACATTCAACG+CGG 0.791435 6:-95161995 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTTGTTTCAACCTGATAAT+TGG + Chr6:95162067-95162086 MsG0680034834.01.T01:CDS 25.0%
ACAAACTTTCTCCAATTATC+AGG - Chr6:95162081-95162100 None:intergenic 30.0%
TGCACATGAACAGATATTAA+AGG + Chr6:95161969-95161988 MsG0680034834.01.T01:CDS 30.0%
TTGTGAAGAAGATGAAAGAA+GGG + Chr6:95162106-95162125 MsG0680034834.01.T01:CDS 30.0%
TTTGTGAAGAAGATGAAAGA+AGG + Chr6:95162105-95162124 MsG0680034834.01.T01:CDS 30.0%
!! GAAAAGTGCCAATAAATGTT+GGG + Chr6:95162128-95162147 MsG0680034834.01.T01:CDS 30.0%
!! TATCAGGTTGAAACAAATCT+TGG - Chr6:95162065-95162084 None:intergenic 30.0%
ACAAATCTTGGTTCATCCTA+AGG - Chr6:95162053-95162072 None:intergenic 35.0%
!! GGAAAAGTGCCAATAAATGT+TGG + Chr6:95162127-95162146 MsG0680034834.01.T01:CDS 35.0%
CAATAAATGTTGGGAGCAAG+TGG + Chr6:95162137-95162156 MsG0680034834.01.T01:CDS 40.0%
CACTTGCTCCCAACATTTAT+TGG - Chr6:95162139-95162158 None:intergenic 40.0%
TAGATGAACCTGTGCTTTCT+GGG - Chr6:95161914-95161933 None:intergenic 40.0%
TGTGTGCATTCACATACCTT+AGG + Chr6:95162034-95162053 MsG0680034834.01.T01:CDS 40.0%
AAAGCACAGGTTCATCTAGC+CGG + Chr6:95161916-95161935 MsG0680034834.01.T01:CDS 45.0%
ACTTGATTCACGAGCTTCTC+CGG - Chr6:95161880-95161899 None:intergenic 45.0%
CTAGATGAACCTGTGCTTTC+TGG - Chr6:95161915-95161934 None:intergenic 45.0%
GTTGAATGTCGAGGGTAACT+CGG + Chr6:95161999-95162018 MsG0680034834.01.T01:CDS 45.0%
TGTGCATGTTCATCATACCG+TGG - Chr6:95161955-95161974 None:intergenic 45.0%
! TTTAGCTACCCAGAAAGCAC+AGG + Chr6:95161903-95161922 MsG0680034834.01.T01:CDS 45.0%
AAGCACAGGTTCATCTAGCC+GGG + Chr6:95161917-95161936 MsG0680034834.01.T01:CDS 50.0%
AATGTTGGGAGCAAGTGGAG+AGG + Chr6:95162142-95162161 MsG0680034834.01.T01:CDS 50.0%
ATGTTGGGAGCAAGTGGAGA+GGG + Chr6:95162143-95162162 MsG0680034834.01.T01:CDS 50.0%
GCAACAGCTTCTTGCACAAG+AGG - Chr6:95162198-95162217 None:intergenic 50.0%
GGAGAAGCTCGTGAATCAAG+TGG + Chr6:95161879-95161898 MsG0680034834.01.T01:CDS 50.0%
GTTACCCTCGACATTCAACG+CGG - Chr6:95161998-95162017 None:intergenic 50.0%
TCAGGATGCACAATGTGCAC+CGG + Chr6:95161858-95161877 MsG0680034834.01.T01:CDS 50.0%
AGCACAGGTTCATCTAGCCG+GGG + Chr6:95161918-95161937 MsG0680034834.01.T01:CDS 55.0%
CCGGGGAAAATGCTTTGCCA+CGG + Chr6:95161935-95161954 MsG0680034834.01.T01:CDS 55.0%
GGTGCACATTGTGCATCCTG+AGG - Chr6:95161859-95161878 None:intergenic 55.0%
!! CCGTGGCAAAGCATTTTCCC+CGG - Chr6:95161938-95161957 None:intergenic 55.0%
GCGTCCGCGTTGAATGTCGA+GGG + Chr6:95161991-95162010 MsG0680034834.01.T01:CDS 60.0%
GCGTGATCACGAGCAGCCTC+AGG + Chr6:95161840-95161859 MsG0680034834.01.T01:CDS 65.0%
GGCGTCCGCGTTGAATGTCG+AGG + Chr6:95161990-95162009 MsG0680034834.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 95161793 95162230 95161793 ID=MsG0680034834.01;Name=MsG0680034834.01
Chr6 mRNA 95161793 95162230 95161793 ID=MsG0680034834.01.T01;Parent=MsG0680034834.01;Name=MsG0680034834.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|145
Chr6 exon 95161793 95162230 95161793 ID=MsG0680034834.01.T01:exon:6276;Parent=MsG0680034834.01.T01
Chr6 CDS 95161793 95162230 95161793 ID=MsG0680034834.01.T01:cds;Parent=MsG0680034834.01.T01
Gene Sequence

>MsG0680034834.01.T01

ATGTTGGCGAGACACGGTGCTATTTTCAACTTCACATGTATTGAGATGCGTGATCACGAGCAGCCTCAGGATGCACAATGTGCACCGGAGAAGCTCGTGAATCAAGTGGCTTTAGCTACCCAGAAAGCACAGGTTCATCTAGCCGGGGAAAATGCTTTGCCACGGTATGATGAACATGCACATGAACAGATATTAAAGGCGTCCGCGTTGAATGTCGAGGGTAACTCGGATGAGACAGAGATGTGTGCATTCACATACCTTAGGATGAACCAAGATTTGTTTCAACCTGATAATTGGAGAAAGTTTGTAGCATTTGTGAAGAAGATGAAAGAAGGGAAAAGTGCCAATAAATGTTGGGAGCAAGTGGAGAGGGAAGCTGAGCATTTTGTGCATGTTACACAGCCTCTTGTGCAAGAAGCTGTTGCTATGATGCACTAA
Protein sequence

>MsG0680034834.01.T01

MLARHGAIFNFTCIEMRDHEQPQDAQCAPEKLVNQVALATQKAQVHLAGENALPRYDEHAHEQILKASALNVEGNSDETEMCAFTYLRMNQDLFQPDNWRKFVAFVKKMKEGKSANKCWEQVEREAEHFVHVTQPLVQEAVAMMH*