AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035054.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035054.01.T01 MTR_3g099580 79.817 109 22 0 1 109 13 121 1.32e-60 184
MsG0680035054.01.T01 MTR_3g099570 74.312 109 28 0 1 109 13 121 2.97e-56 172
MsG0680035054.01.T01 MTR_3g099680 72.072 111 29 2 1 109 13 123 1.12e-51 162
MsG0680035054.01.T01 MTR_3g099980 72.072 111 29 2 1 109 13 123 5.77e-51 160
MsG0680035054.01.T01 MTR_3g099540 58.182 110 46 0 1 110 14 123 2.40e-41 133
MsG0680035054.01.T01 MTR_8g007035 52.830 106 47 2 6 108 15 120 5.66e-33 114
MsG0680035054.01.T01 MTR_8g007020 54.455 101 44 1 10 108 20 120 7.04e-33 113
MsG0680035054.01.T01 MTR_4g114870 48.000 100 47 2 14 108 191 290 3.47e-25 97.4
MsG0680035054.01.T01 MTR_4g114870 49.020 102 47 2 12 108 27 128 1.26e-24 95.9
MsG0680035054.01.T01 MTR_1g090190 41.414 99 58 0 11 109 20 118 4.20e-25 93.6
MsG0680035054.01.T01 MTR_2g088990 43.878 98 54 1 10 106 21 118 9.68e-24 91.3
MsG0680035054.01.T01 MTR_6g080660 41.237 97 57 0 14 110 28 124 5.38e-23 89.0
MsG0680035054.01.T01 MTR_5g006040 44.554 101 53 3 11 108 23 123 4.52e-22 85.9
MsG0680035054.01.T01 MTR_0334s0010 41.509 106 57 3 9 110 23 127 4.96e-22 86.3
MsG0680035054.01.T01 MTR_1g104800 41.748 103 57 3 9 108 20 122 1.28e-21 84.7
MsG0680035054.01.T01 MTR_6g023760 41.000 100 58 1 10 108 22 121 1.80e-21 84.3
MsG0680035054.01.T01 MTR_8g099220 40.777 103 57 2 11 109 26 128 3.84e-21 82.0
MsG0680035054.01.T01 MTR_1g112700 38.235 102 61 2 11 110 30 131 1.14e-19 79.7
MsG0680035054.01.T01 MTR_7g086140 42.574 101 56 2 10 108 22 122 7.47e-19 77.0
MsG0680035054.01.T01 MTR_7g086190 42.574 101 56 2 10 108 22 122 7.47e-19 77.0
MsG0680035054.01.T01 MTR_1g105130 40.206 97 56 2 14 108 25 121 8.36e-19 76.6
MsG0680035054.01.T01 MTR_1g105120 40.206 97 56 2 14 108 25 121 8.36e-19 76.6
MsG0680035054.01.T01 MTR_6g083240 43.810 105 52 3 11 108 24 128 1.59e-18 76.6
MsG0680035054.01.T01 MTR_2g043730 38.889 108 61 4 1 107 20 123 3.22e-18 74.3
MsG0680035054.01.T01 MTR_7g086160 38.182 110 66 2 1 108 14 123 8.57e-18 74.3
MsG0680035054.01.T01 MTR_3g089035 43.299 97 51 3 11 106 30 123 9.94e-17 70.5
MsG0680035054.01.T01 MTR_4g067200 35.577 104 60 3 14 110 28 131 2.94e-16 70.5
MsG0680035054.01.T01 MTR_4g066110 35.577 104 60 3 14 110 28 131 2.94e-16 70.5
MsG0680035054.01.T01 MTR_2g025580 34.545 110 70 2 2 109 15 124 3.57e-16 70.1
MsG0680035054.01.T01 MTR_7g086220 38.144 97 58 2 14 108 25 121 4.82e-16 69.7
MsG0680035054.01.T01 MTR_4g081100 38.235 102 58 2 16 112 34 135 1.76e-15 69.3
MsG0680035054.01.T01 MTR_2g083250 37.500 104 58 2 16 112 28 131 3.16e-15 69.3
MsG0680035054.01.T01 MTR_6g022170 35.922 103 60 2 11 108 31 132 5.30e-15 67.0
MsG0680035054.01.T01 MTR_7g086100 39.362 94 56 1 16 108 28 121 1.28e-14 67.4
MsG0680035054.01.T01 MTR_7g086090 38.947 95 57 1 16 109 28 122 2.41e-14 66.6
MsG0680035054.01.T01 MTR_8g088830 37.500 104 60 4 5 106 19 119 3.64e-14 63.9
MsG0680035054.01.T01 MTR_2g043790 40.000 100 56 3 9 107 2 98 3.96e-14 63.2
MsG0680035054.01.T01 MTR_8g463180 38.000 100 59 2 16 112 26 125 5.43e-14 66.2
MsG0680035054.01.T01 MTR_4g007250 35.922 103 64 2 8 109 23 124 1.04e-13 63.9
MsG0680035054.01.T01 MTR_8g089110 38.776 98 56 3 11 107 25 119 1.71e-13 62.0
MsG0680035054.01.T01 MTR_7g086230 37.895 95 58 1 16 109 28 122 2.05e-13 63.2
MsG0680035054.01.T01 MTR_7g086280 37.895 95 58 1 16 109 28 122 2.18e-13 63.2
MsG0680035054.01.T01 MTR_4g077787 32.432 111 68 2 4 108 22 131 2.34e-13 63.2
MsG0680035054.01.T01 MTR_3g089005 39.216 102 58 3 6 106 25 123 3.04e-13 61.6
MsG0680035054.01.T01 MTR_4g111640 33.010 103 62 2 12 108 30 131 3.82e-13 62.4
MsG0680035054.01.T01 MTR_2g101300 34.286 105 63 2 11 110 24 127 6.50e-13 62.0
MsG0680035054.01.T01 MTR_2g090575 37.500 96 57 2 16 108 27 122 6.79e-13 63.5
MsG0680035054.01.T01 MTR_1g090420 32.479 117 70 3 1 108 10 126 8.46e-13 62.4
MsG0680035054.01.T01 MTR_1g014120 33.663 101 62 2 17 112 29 129 2.61e-12 60.5
MsG0680035054.01.T01 MTR_4g112310 35.802 81 51 1 28 108 2 81 2.76e-12 59.3
MsG0680035054.01.T01 MTR_4g124280 34.906 106 64 2 10 110 21 126 3.47e-12 60.8
MsG0680035054.01.T01 MTR_4g130780 35.714 84 53 1 28 110 48 131 3.97e-12 60.1
MsG0680035054.01.T01 MTR_4g078410 35.000 100 62 2 16 112 27 126 4.60e-12 59.7
MsG0680035054.01.T01 MTR_8g086360 35.000 100 62 2 16 112 26 125 9.42e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035054.01.T01 AT1G72230 53.465 101 47 0 10 110 19 119 1.78e-37 125
MsG0680035054.01.T01 AT1G22480 57.609 92 39 0 19 110 24 115 4.37e-33 114
MsG0680035054.01.T01 AT3G60270 46.465 99 53 0 10 108 22 120 3.02e-27 99.4
MsG0680035054.01.T01 AT2G32300 42.574 101 57 1 9 108 21 121 3.07e-25 95.9
MsG0680035054.01.T01 AT2G32300 42.574 101 57 1 9 108 51 151 6.05e-25 95.5
MsG0680035054.01.T01 AT2G26720 46.000 100 52 2 11 108 26 125 1.35e-24 92.8
MsG0680035054.01.T01 AT5G07475 42.424 99 57 0 11 109 27 125 7.77e-24 90.5
MsG0680035054.01.T01 AT5G26330 46.535 101 51 2 11 108 21 121 2.66e-23 89.0
MsG0680035054.01.T01 AT3G27200 38.393 112 65 2 1 108 10 121 1.37e-22 87.0
MsG0680035054.01.T01 AT2G44790 47.826 92 46 1 19 108 33 124 1.78e-22 87.4
MsG0680035054.01.T01 AT2G31050 45.000 100 53 2 11 108 26 125 4.42e-22 86.3
MsG0680035054.01.T01 AT3G60280 40.404 99 59 0 10 108 20 118 6.97e-20 81.3
MsG0680035054.01.T01 AT3G17675 37.037 108 67 1 2 108 20 127 1.76e-18 75.1
MsG0680035054.01.T01 AT3G18590 35.577 104 60 2 11 108 24 126 1.89e-14 66.2
MsG0680035054.01.T01 AT1G64640 36.190 105 63 2 11 111 30 134 2.03e-14 66.2
MsG0680035054.01.T01 AT5G20230 40.206 97 54 2 16 108 27 123 9.43e-14 64.3
MsG0680035054.01.T01 AT2G25060 33.333 117 73 2 1 112 19 135 2.05e-13 63.5
MsG0680035054.01.T01 AT5G53870 31.624 117 69 2 7 112 15 131 3.28e-13 64.7
MsG0680035054.01.T01 AT5G53870 31.624 117 69 2 7 112 15 131 3.51e-13 64.3
MsG0680035054.01.T01 AT4G28365 34.694 98 61 2 16 110 31 128 3.78e-13 62.8
MsG0680035054.01.T01 AT5G57920 38.554 83 50 1 29 110 43 125 4.24e-13 62.4
MsG0680035054.01.T01 AT5G57920 38.554 83 50 1 29 110 42 124 5.49e-13 62.4
MsG0680035054.01.T01 AT5G57920 38.554 83 50 1 29 110 75 157 6.54e-13 62.4
MsG0680035054.01.T01 AT1G45063 36.000 100 60 3 13 108 148 247 8.40e-13 62.8
MsG0680035054.01.T01 AT3G20570 36.449 107 64 2 10 112 26 132 9.23e-13 62.0
MsG0680035054.01.T01 AT4G30590 34.118 85 55 1 29 112 48 132 1.00e-12 61.6
MsG0680035054.01.T01 AT1G45063 36.000 100 60 3 13 108 148 247 1.19e-12 63.2
MsG0680035054.01.T01 AT4G12880 34.314 102 63 3 11 109 26 126 4.12e-12 58.9
MsG0680035054.01.T01 AT2G02850 38.384 99 53 4 11 106 33 126 8.82e-12 58.2
MsG0680035054.01.T01 AT1G48940 34.694 98 57 2 17 108 29 125 2.60e-11 57.8
MsG0680035054.01.T01 AT4G32490 31.633 98 64 2 16 110 33 130 3.18e-11 58.2
MsG0680035054.01.T01 AT4G31840 32.692 104 64 3 15 112 28 131 6.96e-11 56.6

Find 22 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACCGTTGGGGATACTTT+AGG 0.298621 6:-98245051 MsG0680035054.01.T01:CDS
TGCCATGAAACTCTCTATTA+AGG 0.330487 6:-98244647 MsG0680035054.01.T01:CDS
TACTTCATATGTGCTGTTCC+TGG 0.337108 6:-98244682 MsG0680035054.01.T01:CDS
TTGCAACTGTCCACACCGTT+GGG 0.359738 6:-98245062 MsG0680035054.01.T01:CDS
AACCTTAATAGAGAGTTTCA+TGG 0.393486 6:+98244645 None:intergenic
ACTATGACAGTGTTCAACTA+TGG 0.394246 6:-98244826 MsG0680035054.01.T01:intron
AGTGATAAAACCTTTGCAAT+TGG 0.406507 6:-98244994 MsG0680035054.01.T01:CDS
TTTGCAACTGTCCACACCGT+TGG 0.482096 6:-98245063 MsG0680035054.01.T01:CDS
GATACTTTAGGTTGGACAAT+CGG 0.484942 6:-98245039 MsG0680035054.01.T01:CDS
CAGTGTTCAACTATGGAGTC+GGG 0.538761 6:-98244819 MsG0680035054.01.T01:CDS
TCGGTTCTGATTATAACGCA+TGG 0.542470 6:-98245020 MsG0680035054.01.T01:CDS
ATGGCAGCTATGCAATGTCC+AGG 0.547973 6:+98244664 None:intergenic
GTGTGGACAGTTGCAAAAGT+TGG 0.551081 6:+98245069 None:intergenic
ACAGTGTTCAACTATGGAGT+CGG 0.577018 6:-98244820 MsG0680035054.01.T01:intron
CCGTTGGGGATACTTTAGGT+TGG 0.582085 6:-98245047 MsG0680035054.01.T01:CDS
CGGTTCTGATTATAACGCAT+GGG 0.582902 6:-98245019 MsG0680035054.01.T01:CDS
TGCAACTGTCCACACCGTTG+GGG 0.596271 6:-98245061 MsG0680035054.01.T01:CDS
GAGACTGTCACCAATTGCAA+AGG 0.621682 6:+98244984 None:intergenic
CTAAAGTATCCCCAACGGTG+TGG 0.668084 6:+98245052 None:intergenic
CCAACCTAAAGTATCCCCAA+CGG 0.709587 6:+98245047 None:intergenic
TCAATTAGTACAGACAGTAG+CGG 0.713968 6:-98244742 MsG0680035054.01.T01:CDS
CTATGGAGTCGGGCACACAG+TGG 0.746435 6:-98244809 MsG0680035054.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTTATAGTTCATACTTA+CGG + Chr6:98244761-98244780 None:intergenic 20.0%
!!! TGCATTAGTTTTGTAAATTA+TGG + Chr6:98244865-98244884 None:intergenic 20.0%
!! CTATTAAGGTTAAGAGTTTT+AGG - Chr6:98245087-98245106 MsG0680035054.01.T01:CDS 25.0%
AACCTTAATAGAGAGTTTCA+TGG + Chr6:98245078-98245097 None:intergenic 30.0%
AGTGATAAAACCTTTGCAAT+TGG - Chr6:98244726-98244745 MsG0680035054.01.T01:CDS 30.0%
!!! AATATGTTCCTGCTTTTTTG+AGG + Chr6:98245019-98245038 None:intergenic 30.0%
!!! ATATGTTCCTGCTTTTTTGA+GGG + Chr6:98245018-98245037 None:intergenic 30.0%
ACTATGACAGTGTTCAACTA+TGG - Chr6:98244894-98244913 MsG0680035054.01.T01:intron 35.0%
TCAATTAGTACAGACAGTAG+CGG - Chr6:98244978-98244997 MsG0680035054.01.T01:intron 35.0%
TGCCATGAAACTCTCTATTA+AGG - Chr6:98245073-98245092 MsG0680035054.01.T01:CDS 35.0%
!! GATACTTTAGGTTGGACAAT+CGG - Chr6:98244681-98244700 MsG0680035054.01.T01:CDS 35.0%
!!! TATGTTCCTGCTTTTTTGAG+GGG + Chr6:98245017-98245036 None:intergenic 35.0%
ACAGTGTTCAACTATGGAGT+CGG - Chr6:98244900-98244919 MsG0680035054.01.T01:intron 40.0%
! CGGTTCTGATTATAACGCAT+GGG - Chr6:98244701-98244720 MsG0680035054.01.T01:CDS 40.0%
! TACTTCATATGTGCTGTTCC+TGG - Chr6:98245038-98245057 MsG0680035054.01.T01:CDS 40.0%
! TCGGTTCTGATTATAACGCA+TGG - Chr6:98244700-98244719 MsG0680035054.01.T01:CDS 40.0%
!!! TTTTTTGAGGGGAATGGTTG+TGG + Chr6:98245006-98245025 None:intergenic 40.0%
ACCATTCCCCTCAAAAAAGC+AGG - Chr6:98245008-98245027 MsG0680035054.01.T01:CDS 45.0%
CAGTGTTCAACTATGGAGTC+GGG - Chr6:98244901-98244920 MsG0680035054.01.T01:intron 45.0%
GAGACTGTCACCAATTGCAA+AGG + Chr6:98244739-98244758 None:intergenic 45.0%
GTGTGGACAGTTGCAAAAGT+TGG + Chr6:98244654-98244673 None:intergenic 45.0%
! CCAACCTAAAGTATCCCCAA+CGG + Chr6:98244676-98244695 None:intergenic 45.0%
!!! TCCTGCTTTTTTGAGGGGAA+TGG + Chr6:98245012-98245031 None:intergenic 45.0%
ATGGCAGCTATGCAATGTCC+AGG + Chr6:98245059-98245078 None:intergenic 50.0%
CACACCGTTGGGGATACTTT+AGG - Chr6:98244669-98244688 MsG0680035054.01.T01:CDS 50.0%
CCGTTGGGGATACTTTAGGT+TGG - Chr6:98244673-98244692 MsG0680035054.01.T01:CDS 50.0%
TTGCAACTGTCCACACCGTT+GGG - Chr6:98244658-98244677 MsG0680035054.01.T01:CDS 50.0%
TTTGCAACTGTCCACACCGT+TGG - Chr6:98244657-98244676 MsG0680035054.01.T01:CDS 50.0%
! CTAAAGTATCCCCAACGGTG+TGG + Chr6:98244671-98244690 None:intergenic 50.0%
TGCAACTGTCCACACCGTTG+GGG - Chr6:98244659-98244678 MsG0680035054.01.T01:CDS 55.0%
CTATGGAGTCGGGCACACAG+TGG - Chr6:98244911-98244930 MsG0680035054.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 98244630 98245112 98244630 ID=MsG0680035054.01;Name=MsG0680035054.01
Chr6 mRNA 98244630 98245112 98244630 ID=MsG0680035054.01.T01;Parent=MsG0680035054.01;Name=MsG0680035054.01.T01;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|0|0|2|0|112
Chr6 exon 98244630 98244838 98244630 ID=MsG0680035054.01.T01:exon:7507;Parent=MsG0680035054.01.T01
Chr6 exon 98244983 98245112 98244983 ID=MsG0680035054.01.T01:exon:7506;Parent=MsG0680035054.01.T01
Chr6 CDS 98244983 98245112 98244983 ID=MsG0680035054.01.T01:cds;Parent=MsG0680035054.01.T01
Chr6 CDS 98244630 98244838 98244630 ID=MsG0680035054.01.T01:cds;Parent=MsG0680035054.01.T01
Gene Sequence

>MsG0680035054.01.T01

CTTCTTTTTAGCAATGCACTGCCAACTTTTGCAACTGTCCACACCGTTGGGGATACTTTAGGTTGGACAATCGGTTCTGATTATAACGCATGGGCTAGTGATAAAACCTTTGCAATTGGTGACAGTCTCCTGTTCAACTATGGAGTCGGGCACACAGTGGATGAAGTTAAAGAAAGAGACTATAAATCATGCATAACTAGAAATTCAATTAGTACAGACAGTAGCGGTGCCACAACCATTCCCCTCAAAAAAGCAGGAACATATTACTTCATATGTGCTGTTCCTGGACATTGCATAGCTGCCATGAAACTCTCTATTAAGGTTAAGAGTTTTAGGTGA

Protein sequence

>MsG0680035054.01.T01

LLFSNALPTFATVHTVGDTLGWTIGSDYNAWASDKTFAIGDSLLFNYGVGHTVDEVKERDYKSCITRNSISTDSSGATTIPLKKAGTYYFICAVPGHCIAAMKLSIKVKSFR*